218 results on '"Atsushi Hattori"'
Search Results
2. Long-read next-generation sequencing for molecular diagnosis of pediatric endocrine disorders
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Yoko Kuroki, Atsushi Hattori, Keiko Matsubara, and Maki Fukami
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gene ,molecular analysis ,mutation ,nanopore ,pacbio ,sequencer ,Pediatrics ,RJ1-570 - Abstract
Recent advances in long-read next-generation sequencing (NGS) have enabled researchers to identify several pathogenic variants overlooked by short-read NGS, array-based comparative genomic hybridization, and other conventional methods. Long-read NGS is particularly useful in the detection of structural variants and repeat expansions. Furthermore, it can be used for mutation screening in difficult-to-sequence regions, as well as for DNA-methylation analyses and haplotype phasing. This mini-review introduces the usefulness of long-read NGS in the molecular diagnosis of pediatric endocrine disorders.
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- 2024
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3. Expression levels and DNA methylation profiles of the growth gene SHOX in cartilage tissues and chondrocytes
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Atsushi Hattori, Atsuhito Seki, Naoto Inaba, Kazuhiko Nakabayashi, Kazue Takeda, Kuniko Tatsusmi, Yasuhiro Naiki, Akie Nakamura, Keisuke Ishiwata, Kenji Matsumoto, Michiyo Nasu, Kohji Okamura, Toshimi Michigami, Yuko Katoh-Fukui, Akihiro Umezawa, Tsutomu Ogata, Masayo Kagami, and Maki Fukami
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Medicine ,Science - Abstract
Abstract All attempts to identify male-specific growth genes in humans have failed. This study aimed to clarify why men are taller than women. Microarray-based transcriptome analysis of the cartilage tissues of four adults and chondrocytes of 12 children showed that the median expression levels of SHOX, a growth gene in the pseudoautosomal region (PAR), were higher in male samples than in female samples. Male-dominant SHOX expression was confirmed by quantitative RT-PCR for 36 cartilage samples. Reduced representation bisulfite sequencing of four cartilage samples revealed sex-biased DNA methylation in the SHOX-flanking regions, and pyrosequencing of 22 cartilage samples confirmed male-dominant DNA methylation at the CpG sites in the SHOX upstream region and exon 6a. DNA methylation indexes of these regions were positively correlated with SHOX expression levels. These results, together with prior findings that PAR genes often exhibit male-dominant expression, imply that the relatively low SHOX expression in female cartilage tissues reflects the partial spread of X chromosome inactivation into PAR. Altogether, this study provides the first indication that sex differences in height are ascribed, at least in part, to the sex-dependent epigenetic regulation of SHOX. Our findings deserve further validation.
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- 2024
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4. Maternal uniparental disomy of chromosome 7 underlying argininosuccinic aciduria and Silver-Russell syndrome
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Atsushi Hattori, Torayuki Okuyama, Tetsumin So, Motomichi Kosuga, Keiko Ichimoto, Kei Murayama, Masayo Kagami, Maki Fukami, and Yasuyuki Fukuhara
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Genetics ,QH426-470 ,Life ,QH501-531 - Abstract
Abstract We describe a patient presenting with argininosuccinic aciduria and Silver-Russell syndrome (SRS). SRS was caused by maternal uniparental disomy of chromosome 7 (UPD(7)mat). UPD(7)mat also unmasked a maternally inherited splicing variant in ASL on chromosome 7, leading to the onset of argininosuccinic aciduria. The phenotype of the present case was more severe than that of a previous case, demonstrating a phenotypic variation in the combination of argininosuccinic aciduria and SRS.
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- 2022
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5. Load carrying behavior of concrete reinforced with bundled BFRP bars by using DIC
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Bruno Ribeiro, Hisafumi Asaue, Atsushi Hattori, Tomoki Shiotani, Takumi Kobayashi, Junya Sato, Masakazu Uchida, and Masahiro Ishida
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Basalt fiber-reinforced polymer ,Bundled bars ,Load carrying behavior ,Load-deflection relationship ,Digital image correlation ,Materials of engineering and construction. Mechanics of materials ,TA401-492 - Abstract
Recently, basalt fiber-reinforced polymer (BFRP) has gained attention in the construction industry because of its worldwide availability and eco-friendly nature. Different from conventional steel bars, BFRP bar do not corrode being an ideal reinforcing material in structures surrounding magnetic fields and marine environments, given its chemical stability. This study investigated the load carrying behavior of concrete beams reinforced with conventional deformed steel bars and seven-bundled BFRP bars through four-point flexural test. For each case, two beams were prepared; one beam was designed with a low reinforcement ratio and another with a high reinforcement ratio in order to obtain a load carrying capacity of around 330–368 kN and 483–519 kN, respectively. Deflection, crack width evolution, and strain behavior of each beam were comparatively analyzed using a digital image correlation (DIC) based technique. The DIC analysis results showed that the neutral axis of bundled BFRP reinforced cases move to the upper end in an early loading stage, with larger cracks width, and the deflection is larger in BFRP cases than in those of steel-reinforced beams with the respective reinforcement ratio. However, the four-point flexural test results revealed that bundled BFRP bars can be used as an alternative to conventional steel bars. The load carrying capacities were obtained similarly to designed values, around 364 and 551 kN for low and high reinforcement ratio cases, respectively.
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- 2022
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6. Nuclear Receptor Gene Variants Underlying Disorders/Differences of Sex Development through Abnormal Testicular Development
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Atsushi Hattori and Maki Fukami
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digenic inheritance ,disorders of sex development ,genomic structural variation ,gonadal development ,nuclear receptor ,oligogenicity ,Microbiology ,QR1-502 - Abstract
Gonadal development is the first step in human reproduction. Aberrant gonadal development during the fetal period is a major cause of disorders/differences of sex development (DSD). To date, pathogenic variants of three nuclear receptor genes (NR5A1, NR0B1, and NR2F2) have been reported to cause DSD via atypical testicular development. In this review article, we describe the clinical significance of the NR5A1 variants as the cause of DSD and introduce novel findings from recent studies. NR5A1 variants are associated with 46,XY DSD and 46,XX testicular/ovotesticular DSD. Notably, both 46,XX DSD and 46,XY DSD caused by the NR5A1 variants show remarkable phenotypic variability, to which digenic/oligogenic inheritances potentially contribute. Additionally, we discuss the roles of NR0B1 and NR2F2 in the etiology of DSD. NR0B1 acts as an anti-testicular gene. Duplications containing NR0B1 result in 46,XY DSD, whereas deletions encompassing NR0B1 can underlie 46,XX testicular/ovotesticular DSD. NR2F2 has recently been reported as a causative gene for 46,XX testicular/ovotesticular DSD and possibly for 46,XY DSD, although the role of NR2F2 in gonadal development is unclear. The knowledge about these three nuclear receptors provides novel insights into the molecular networks involved in the gonadal development in human fetuses.
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- 2023
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7. Lupus anticoagulant hypoprothrombinemia syndrome associated with bilateral adrenal haemorrhage in a child: early diagnosis and intervention
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Atsushi Sakamoto, Masao Ogura, Atsushi Hattori, Kinji Tada, Reiko Horikawa, Hisaya Nakadate, Kimikazu Matsumoto, Keiji Nogami, Masahiro Ieko, and Akira Ishiguro
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Lupus anticoagulant ,Hypoprothrombinemia ,Adrenal haemorrhage ,Adrenal insufficiency ,Lupus anticoagulant hypoprothrombinemia syndrome ,Diseases of the blood and blood-forming organs ,RC633-647.5 - Abstract
Abstract Background Lupus anticoagulant-hypoprothrombinemia syndrome (LAHPS) is characterized by bleeding and thrombosis in patients with autoimmune diseases or infections. Paediatric LAHPS exhibits various degrees of bleeding, ranging from mild to severe; however, adrenal haemorrhage due to LAHPS and its long-term clinical course have not been sufficiently described. Case presentation A 9-year-old boy presented with prolonged abdominal pain and abnormal coagulation screening tests. The laboratory tests showed prolonged activated partial thromboplastin time and subsequently revealed the presence of lupus anticoagulant, anti-nuclear antibodies, and hypoprothrombinemia, leading to diagnosis of LAHPS. An enhanced computed tomogram demonstrated nodular lesions in the adrenal glands bilaterally, suggestive of adrenal haemorrhage. Laboratory and clinical manifestations exhibited life-threatening adrenal insufficiency that required hydrocortisone administration. The patient developed systemic lupus erythematosus, diagnosed 12 months later. Conclusions This patient with LAHPS developed rare adrenal failure due to adrenal haemorrhage, a life-threatening event that should be recognized and treated early. In our case, renal dysfunction was also observed when systemic lupus erythematosus was diagnosed 1 year after LAHPS. Our case emphasizes that early recognition of adrenal failure and careful long-term observation is required in patients with autoantibodies.
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- 2021
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8. NDNF variants are rare in patients with congenital hypogonadotropic hypogonadism
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Satoshi Tamaoka, Erina Suzuki, Atsushi Hattori, Tsutomu Ogata, Maki Fukami, and Yuko Katoh-Fukui
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Genetics ,QH426-470 ,Life ,QH501-531 - Abstract
Abstract Although NDNF was recently reported as a novel causative gene for congenital hypogonadotropic hypogonadism (CHH), this conclusion has yet to be validated. In this study, we sequenced NDNF in 61 Japanese CHH patients. No variants, except for nine synonymous substitutions that appear to have no effect on splice-site recognition, were identified in NDNF coding exons or flanking intronic sequences. These results indicate the rarity of NDNF variants in CHH patients and highlight the genetic heterogeneity of CHH.
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- 2021
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9. Transient multifocal genomic crisis creating chromothriptic and non-chromothriptic rearrangements in prezygotic testicular germ cells
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Atsushi Hattori, Kohji Okamura, Yumiko Terada, Rika Tanaka, Yuko Katoh-Fukui, Yoichi Matsubara, Keiko Matsubara, Masayo Kagami, Reiko Horikawa, and Maki Fukami
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Chromoanagenesis ,Chromosomal instability ,Chromothripsis ,Comparative genomic hybridization ,Complex genomic rearrangement ,Copy number variation ,Internal medicine ,RC31-1245 ,Genetics ,QH426-470 - Abstract
Abstract Background The co-occurrence of multiple de novo copy number variations (CNVs) is a rare phenomenon in the human genome. Recently, an “organismal CNV mutator phenotype” has been reported to result in transient genomic instability introducing multiple de novo CNVs in primary oocytes and early-stage zygotes. These findings opened a new area of human genome research. Methods We performed genome-wide copy number analysis for ~ 2100 individuals with various congenital defects. Furthermore, extensive molecular analyses, including synthetic long-read whole-genome sequencing and haplotype-phasing, were carried out for an individual with multiple de novo CNVs. Results A boy was found to have de novo rearrangements on five chromosomes. The rearrangements comprised simple duplication and inversion as well as chaotic changes, all of which affected paternally derived chromosomes. Postzygotic genomic instability was ruled out. The duplicated regions on 6q and 13q contained both diallelic and triallelic loci, indicating that the genomic rearrangements were initially created during premeiotic mitosis and subsequently modified by physiological cross-over during meiosis I. Breakpoints of the rearrangements were indicative of non-homologous end joining, replication-based errors, and/or chromothripsis. The mutagenic event was independent of specific local DNA motifs or de novo point mutations, but may be driven by spermatogenesis-specific factors. Conclusions These results indicate that during spermatogenesis, a transient multifocal genomic crisis can introduce several chromothriptic and non-chromothriptic changes into the genome. These findings broaden the concept of the “organismal CNV mutator phenotype”. This study provides insights into mechanisms for altering the global chromosomal architecture of human embryos.
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- 2019
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10. Detection of elemental magnetization reversal events in a micro-patterned Nd-Fe-B hot-deformed magnet
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Takahiro Yomogita, Nobuaki Kikuchi, Satoshi Okamoto, Osamu Kitakami, Hossein Sepehri-Amin, Tadakatsu Ohkubo, Kazuhiro Hono, Keiko Hioki, and Atsushi Hattori
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Physics ,QC1-999 - Abstract
Magnetization reversal in a permanent magnet takes place through multiple and simultaneous events of nucleation and domain wall depinning. Thus, detection and analysis of elemental magnetization reversal events are essentially important to understand the coercivity mechanism of a permanent magnet. In this study, we have fabricated a micro-patterned Nd-Fe-B hot-deformed magnet using mechanical polishing and focused ion beam, and anomalous Hall effect (AHE) detection has been adopted to measure the magnetization reversal of the sample. During the micro-patterning process, the degradation of magnetic property is carefully evaluated. Consequently, the micro-patterned Nd-Fe-B hot-deformed magnet with the thick of 5 μm and the width of 13 μm is fabricated, and subsequently, the discrete steps on the AHE curve are successfully detected. The magnetization reversal unit size estimated from the step height is ∼1 μm2, which is almost the same as observed in the magneto-optical Kerr microscopy. We have clearly demonstrated that this technique has significant potential to study the physical nature of elemental magnetization reversal events in permanent magnets.
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- 2019
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11. An Automatic Image Collection System for Multicenter Clinical Studies.
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Toshihiro Takeda, Shirou Manabe, Atsushi Hattori, Junji Yamaguchi, Shozo Konishi, Yuichiro Yamamoto, Daiyo Takahashi, and Yasushi Matsumura
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- 2020
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12. RE-EVALUATION OF REGIONAL PLANS IN OKINAWA DESIGNED BY ATELIER ZO (PART 1): MUTUAL COMPARISON OF PLANS IN ONNA, NAGO AND NAKIJIN, AND VERIFICATION OF THE PROCESS OF ESTABLISHING THOSE PLANS
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Atsushi HATTORI, Kiichi MIYAJI, and Wataru KOSAKA
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General Medicine - Published
- 2023
13. Case Report Form Reporter: A Key Component for the Integration of Electronic Medical Records and the Electronic Data Capture System.
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Yasushi Matsumura, Atsushi Hattori, Shiro Manabe, Daiyo Takahashi, Yuichiro Yamamoto, Taizo Murata, Akito Nakagawa, Naoki Mihara, and Toshihiro Takeda
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- 2017
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14. A Strategy for Reusing the Data of Electronic Medical Record Systems for Clinical Research.
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Yasushi Matsumura, Atsushi Hattori, Shiro Manabe, Tsutomu Tsuda, Toshihiro Takeda, Katsuki Okada, Taizo Murata, and Naoki Mihara
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- 2016
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15. 小径倣いプローブと画像測定機のハイブリット化が拓く新しい微細形状測定
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Atsushi HATTORI, Takaharu IMURA, Hideyuki ARAI, and So ITO
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Mechanical Engineering - Published
- 2022
16. Chromosomal microdeletion leading to pituitary gigantism through hormone-gene overexpression
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Yuko Katoh-Fukui, Atsushi Hattori, Ruogu Zhang, Miho Terao, Shuji Takada, Kazuhiko Nakabayashi, Kenichiro Hata, Yutaka Yamada, Nobuo Matsuura, and Maki Fukami
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Genetics ,General Medicine ,Molecular Biology ,Genetics (clinical) - Abstract
Pituitary gigantism is a rare endocrinopathy characterized by tall stature due to growth hormone (GH) hypersecretion. This condition is generally linked to a genetic predisposition to tumors that produce GH or GH-releasing hormone (GHRH). Here, we report a Japanese woman who exhibited prominent body growth from infancy to reach an adult height of 197.4 cm (+7.4 standard deviation). Her blood GH levels were markedly elevated. She carried no pathogenic variants in known growth-controlling genes but had a hitherto unreported 752 kb heterozygous deletion at 20q11.23. The microdeletion was located 8.9 kb upstream of GHRH and encompassed exons 2–9 of a ubiquitously expressed gene TTI1 together with 12 other genes, pseudogenes and non-coding RNAs. Transcript analyses of the patient’s leukocytes showed that the microdeletion produced chimeric mRNAs consisting of exon 1 of TTI1 and all coding exons of GHRH. In silico analysis detected promoter-associated genomic features around TTI1 exon 1. Genome-edited mice carrying the same microdeletion recapitulated accelerated body growth from a few weeks after birth. The mutant mice developed pituitary hyperplasia and exhibited ectopic Ghrh expression in all tissues examined. Thus, the extreme phenotype of pituitary gigantism in the patient likely reflects GHRH overexpression driven by an acquired promoter. The results of this study indicate that germline submicroscopic deletions have the potential to cause conspicuous developmental abnormalities due to gene overexpression. Furthermore, this study provides evidence that constitutive expression of a hormone-encoding gene can result in congenital disease.
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- 2023
17. Practical use of a multicenter clinical research support system connected to electronic medical records.
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Shirou Manabe, Toshihiro Takeda, Atsushi Hattori, Masashi Yamamoto, Yoshie Shimai, Yoshiki Namiuchi, Junji Yamaguchi, Tomomi Yamada, Shozo Konishi, and Yasushi Matsumura
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- 2021
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18. Interconnection of Electronic Medical Record with Clinical Data Management System by CDISC ODM.
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Yasushi Matsumura, Atsushi Hattori, Shiro Manabe, Toshihiro Takeda, Daiyo Takahashi, Yuichiro Yamamoto, Taizo Murata, and Naoki Mihara
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- 2014
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19. Quantification of Maternal Microchimeric Cells in the Liver of Children With Biliary Atresia
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Satoshi Tamaoka, Akinari Fukuda, Yuko Katoh-Fukui, Atsushi Hattori, Hajime Uchida, Seiichi Shimizu, Yusuke Yanagi, Sami B. Kanaan, Seisuke Sakamoto, Mureo Kasahara, Takako Yoshioka, and Maki Fukami
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Liver ,Biliary Atresia ,Pediatrics, Perinatology and Child Health ,Gastroenterology ,Humans ,Child ,Chimerism ,Liver Transplantation - Abstract
Biliary atresia (BA) is a rare disorder of unknown etiology. There is a debate as to whether maternal microchimerism plays a significant role in the development of BA or in graft tolerance after liver transplantation. Here, we performed quantitative-PCR-based assays for liver tissues of children with BA and other diseases. Maternal cells were detected in 4/13 and 1/3 of the BA and control groups, respectively. The estimated number of maternal cells ranged between 0 and 34.7 per 106 total cells. The frequency and severity of maternal microchimerism were similar between the BA and control groups, and between patients with and without acute rejection of maternal grafts. These results highlight the high frequency of maternal microchimerism in the liver. This study provides no evidence for roles of microchimerism in the etiology of BA or in graft tolerance. Thus, the biological consequences of maternal microchimerism need to be clarified in future studies.
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- 2022
20. Agents from Reality.
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Kazuhiro Asai, Atsushi Hattori, Katsuya Yamashita, Takashi Nishimoto, Yoshifumi Kitamura, and Fumio Kishino
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- 2005
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21. EFFECT OF CABLE RUPTURE NEAR ANCHORAGE ZONE ON LOAD BEARING PERFORMANCE OF PC BOX GIRDER BRIDGE
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Eiji YOSHIDA, Yoshinobu OSHIMA, Masahiro ISHIDA, Takashi YAMAMOTO, Atsushi HATTORI, and Yoshikazu TAKAHASHI
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- 2021
22. Incremental Learning and Forgetting in RBF Networks and SVMs with Applications to Financial Problems.
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Hirotaka Nakayama and Atsushi Hattori
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- 2003
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23. Efficiency of Impressed Current Cathodic Protection in Repaired Reinforced Concrete
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Atsushi Hattori, Kawano Hirotaka, Nadia Kamaruddin, and Rohaya Abdul Malek
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Materials science ,Mechanics of Materials ,Mechanical Engineering ,Forensic engineering ,General Materials Science ,Condensed Matter Physics ,Reinforced concrete ,Cathodic protection - Abstract
—Corrosion attack in reinforced concrete and thus contribute to degradation of concrete influenced by aggressive environment, represent a great concern in relation to the durability of reinforced concrete structures. In this respect, an interesting need is required that is patch repair with additional protection known as ICCP method to prolong the serviceability of the concrete structures. However, details inspection is crucial to understand the effect of different repairing materials and chloride content towards application of ICCP. To clarify this issue, non-protected and protected test specimens were prepared, and analyzed up to 175 days of protection. The result reveals that, reference specimen was found as most corroded specimen unable to obtain 100 mV depolarization at 56 days installation compared to repaired ones. However, all specimens show only greater depolarization values beyond 129 days of installation. To clarity further effectiveness of ICCP system to the studied specimens, time-dependence within 24 hours disconnecting CP current has been carried out and depolarization values was recorded every 4 hours interval. In the final analysis, all specimens are satisfying the CP criteria by obtaining depolarization potential ranging 100 to 250 mV at 4 and 24 hours monitoring period. Index Terms— corrosion attacks, reinforced concrete, repaired concrete, repairing materials, impressed current
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- 2020
24. Rare variant of the epigenetic regulator SMCHD1 in a patient with pituitary hormone deficiency
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Maki Fukami, Keisuke Nagasaki, Kazuhiko Nakabayashi, Tsutomu Ogata, Mami Miyado, Atsushi Hattori, Erina Suzuki, Koji Nagao, Keisuke Ishiwata, Kenichi Kinjo, Chikashi Obuse, Ryu-Suke Nozawa, Kenji Miyado, and Koji Muroya
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Male ,Isolated hypogonadotropic hypogonadism ,medicine.medical_specialty ,Adolescent ,Chromosomal Proteins, Non-Histone ,lcsh:Medicine ,Diseases ,Locus (genetics) ,Bioinformatics ,Microphthalmia ,Hypopituitarism ,Article ,Epigenesis, Genetic ,Young Adult ,Endocrinology ,Medical research ,Exome Sequencing ,Genetics ,Humans ,Medicine ,Computer Simulation ,Genetic Testing ,Epigenetics ,lcsh:Science ,Genetic Association Studies ,Exome sequencing ,Genetic testing ,Optic nerve hypoplasia ,Multidisciplinary ,Molecular medicine ,medicine.diagnostic_test ,business.industry ,Hypogonadism ,lcsh:R ,DNA Methylation ,Middle Aged ,medicine.disease ,Pedigree ,Amino Acid Substitution ,Medical genetics ,Female ,lcsh:Q ,business - Abstract
Isolated hypogonadotropic hypogonadism (IHH), combined pituitary hormone deficiency (CPHD), and septo-optic dysplasia (SOD) constitute a disease spectrum whose etiology remains largely unknown. This study aimed to clarify whether mutations in SMCHD1, an epigenetic regulator gene, might underlie this disease spectrum. SMCHD1 is a causative gene for Bosma arhinia microphthalmia syndrome characterized by arhinia, microphthalmia and IHH. We performed mutation screening of SMCHD1 in patients with etiology-unknown IHH (n = 31) or CPHD (n = 43, 19 of whom also satisfied the SOD diagnostic criteria). Rare variants were subjected to in silico analyses and classified according to the American College of Medical Genetics and Genomics guidelines. Consequently, a rare likely pathogenic variant, p.Asp398Asn, was identified in one patient. The patient with p.Asp398Asn exhibited CPHD, optic nerve hypoplasia, and a thin retinal nerve fiber layer, and therefore satisfied the criteria of SOD. This patient showed a relatively low DNA methylation level of the 52 SMCHD1-target CpG sites at the D4Z4 locus. Exome sequencing for the patient excluded additional variants in other IHH/CPHD-causative genes. In vitro assays suggested functional impairment of the p.Asp398Asn variant. These results provide the first indication that SMCHD1 mutations represent a rare genetic cause of the HH-related disease spectrum.
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- 2020
25. 45,X/46,X,psu idic(Y)(q11.2) in a phenotypically normal male with short stature: a case report
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Maki Fukami, Atsushi Hattori, Mihoko Yamaguchi, Satoshi Miyagaki, Yasuhiro Kawabe, Takeshi Ota, Jun Mori, and Hidechika Morimoto
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medicine.medical_specialty ,Endocrinology, Diabetes and Metabolism ,Urethroplasty ,medicine.medical_treatment ,Genital anomalies ,Case Report ,030209 endocrinology & metabolism ,Y chromosome ,Short stature ,Y chromosome deletion ,03 medical and health sciences ,0302 clinical medicine ,Endocrinology ,Chromosome analysis ,Medicine ,030212 general & internal medicine ,Gynecology ,business.industry ,46,X,psu idic(Y)(q11.2)/45,X,idic(Y) ,Bone age ,Radiological examination ,short stature ,Pediatrics, Perinatology and Child Health ,growth-control gene ,medicine.symptom ,business ,Comparative genomic hybridization - Abstract
We report a case of 15-yr-old phenotypically normal male with short stature associated with the chromosomal abnormalities of 46,X,psu idic(Y)(q11.2)/45,X. At 3 yr of age, he underwent urethroplasty for scrotal hypospadias. At 15 yr of age, he was referred to our hospital due to short stature (–3.71 SD). The results of blood examination were mostly normal. A radiological examination revealed his bone age was 15.7 yr (based on the TW2-RUS method). Chromosome analysis of peripheral lymphocytes revealed 46,X,psu idic(Y)(q11.2)[16]/45,X[14], and array comparative genomic hybridization (aCGH) showed a large deletion of Yq which was located distal to the Y chromosome growth-control gene (GCY) region. It is likely that these structural abnormalities in the Y chromosome were responsible for the short stature. This case might provide new insights regarding GCY and emphasizes the importance of chromosome analysis in not only females but also males with short stature, especially when associated with genital anomalies.
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- 2020
26. Established and Novel Mechanisms Leading to de novo Genomic Rearrangements in the Human Germline
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Atsushi Hattori and Maki Fukami
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Recombination, Genetic ,Genetics ,Chromothripsis ,0303 health sciences ,030305 genetics & heredity ,Specific time ,Non-allelic homologous recombination ,Embryonic Development ,Retrotransposon ,Chromoplexy ,Biology ,Germline ,03 medical and health sciences ,Oogenesis ,Humans ,Human genome ,Copy-number variation ,Spermatogenesis ,Molecular Biology ,Germ-Line Mutation ,Genetics (clinical) ,030304 developmental biology - Abstract
During gametogenesis, the human genome can acquire various de novo rearrangements. Most constitutional genomic rearrangements are created through 1 of the 4 well-known mechanisms, i.e., nonallelic homologous recombination, erroneous repair after double-strand DNA breaks, replication errors, and retrotransposition. However, recent studies have identified 2 types of extremely complex rearrangements that cannot be simply explained by these mechanisms. The first type consists of chaotic structural changes in 1 or a few chromosomes that result from “chromoanagenesis (an umbrella term that covers chromothripsis, chromoanasynthesis, and chromoplexy).” The other type is large independent rearrangements in multiple chromosomes indicative of “transient multifocal genomic crisis.” Germline chromoanagenesis (chromothripsis) likely occurs predominantly during spermatogenesis or postzygotic embryogenesis, while multifocal genomic crisis appears to be limited to a specific time window during oogenesis and early embryogenesis or during spermatogenesis. This review article introduces the current understanding of the molecular basis of de novo rearrangements in the germline.
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- 2020
27. Chloride ion Penetration Properties and Repairing Strategy of Concrete with Partially Damaged Surface Coating
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Hirotaka Kawano, Atsushi Hattori, Risa Matsumoto, and Keiichiro Nakamura
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Surface coating ,Materials science ,Mechanics of Materials ,Mechanical Engineering ,General Materials Science ,Composite material ,Condensed Matter Physics ,Chloride ion penetration - Published
- 2019
28. Pseudoautosomal gene SHOX exhibits sex-biased random monoallelic expression and contributes to sex difference in height
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Akihiro Umezawa, Michiyo Nasu, Tsutomu Ogata, Maki Fukami, Kazuhiko Nakabayashi, Toshimi Michigami, Atsushi Hattori, Kenji Matsumoto, Kazue Takeda, Yasuhiro Naiki, Kohji Okamura, Akie Nakamura, Yuko Katoh-Fukui, Kuniko Tatsusmi, Keisuke Ishiwata, Masayo Kagami, Naoto Inaba, and Atsuhito Seki
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Regulation of gene expression ,Genetics ,Autosome ,DNA methylation ,Pseudoautosomal region ,Chromosome ,Epigenetics ,Biology ,Gene ,X-inactivation - Abstract
Adult men are, on average, ∼13 cm taller than adult women. Although previous studies have suggested a significant contribution of sex chromosomal genes to sexual dimorphism in height, all attempts to identify a male-specific growth gene have failed. In the present study, we analyzed transcripts from cartilage tissues, and found that the expression of SHOX, a growth-promoting gene in the pseudoautosomal region on the X and Y chromosomes, was lower in females than in males. DNA methylation analyses showed that SHOX has some characteristics of genes subjected to X chromosome inactivation (XCI). These findings indicate that sex difference in human height is mainly ascribed to incomplete spreading of XCI on a pseudoautosomal gene. More importantly, RT-PCR of fibroblast clones revealed XCI-independent random clonal monoallelic expression of SHOX. We presume that during eutherian evolution, SHOX translocated from an autosome to the proto-sex chromosome without losing the epigenetic memory of random clonal monoallelic expression and subsequently underwent partial XCI. This study provides a novel model of epigenetic gene regulation leading to phenotypic diversity in humans.
- Published
- 2021
29. Transient multifocal genomic crisis creating chromothriptic and non-chromothriptic rearrangements in prezygotic testicular germ cells
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Yoichi Matsubara, Keiko Matsubara, Yumiko Terada, Yuko Katoh-Fukui, Rika Tanaka, Atsushi Hattori, Masayo Kagami, Maki Fukami, Kohji Okamura, and Reiko Horikawa
- Subjects
Adult ,Male ,0301 basic medicine ,Genome instability ,Genotyping ,lcsh:Internal medicine ,Genomic imprinting ,DNA Copy Number Variations ,lcsh:QH426-470 ,Zygote ,Copy number analysis ,Biology ,Chromoanagenesis ,Genome ,03 medical and health sciences ,0302 clinical medicine ,Chromosome instability ,Testis ,Gene duplication ,Haplotype ,Genetics ,Humans ,Copy-number variation ,lcsh:RC31-1245 ,Genetics (clinical) ,Gene Rearrangement ,Chromothripsis ,Comparative genomic hybridization ,Copy number variation ,Genomics ,Middle Aged ,Spermatozoa ,Chromosomal instability ,lcsh:Genetics ,030104 developmental biology ,Haplotypes ,Whole genome sequencing ,030220 oncology & carcinogenesis ,Female ,Complex genomic rearrangement ,Research Article - Abstract
Background The co-occurrence of multiple de novo copy number variations (CNVs) is a rare phenomenon in the human genome. Recently, an “organismal CNV mutator phenotype” has been reported to result in transient genomic instability introducing multiple de novo CNVs in primary oocytes and early-stage zygotes. These findings opened a new area of human genome research. Methods We performed genome-wide copy number analysis for ~ 2100 individuals with various congenital defects. Furthermore, extensive molecular analyses, including synthetic long-read whole-genome sequencing and haplotype-phasing, were carried out for an individual with multiple de novo CNVs. Results A boy was found to have de novo rearrangements on five chromosomes. The rearrangements comprised simple duplication and inversion as well as chaotic changes, all of which affected paternally derived chromosomes. Postzygotic genomic instability was ruled out. The duplicated regions on 6q and 13q contained both diallelic and triallelic loci, indicating that the genomic rearrangements were initially created during premeiotic mitosis and subsequently modified by physiological cross-over during meiosis I. Breakpoints of the rearrangements were indicative of non-homologous end joining, replication-based errors, and/or chromothripsis. The mutagenic event was independent of specific local DNA motifs or de novo point mutations, but may be driven by spermatogenesis-specific factors. Conclusions These results indicate that during spermatogenesis, a transient multifocal genomic crisis can introduce several chromothriptic and non-chromothriptic changes into the genome. These findings broaden the concept of the “organismal CNV mutator phenotype”. This study provides insights into mechanisms for altering the global chromosomal architecture of human embryos. Electronic supplementary material The online version of this article (10.1186/s12920-019-0526-3) contains supplementary material, which is available to authorized users.
- Published
- 2019
30. DNA Methylation Status of SHOX-Flanking CpG Islands in Healthy Individuals and Short Stature Patients with Pseudoautosomal Copy Number Variations
- Author
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Atsushi Hattori, Masako Izawa, Hitoshi Yamamoto, Hirohito Shima, Kenichiro Ogushi, Shun Soneda, Akihiro Umezawa, Reiko Horikawa, Hideaki Yagasaki, Erina Suzuki, Tomohiro Ishii, Noriyuki Namba, Mami Miyado, Toshiaki Tanaka, Koji Muroya, Yasuhiro Hirano, Keiko Matsubara, Masayo Kagami, Kimiaki Uetake, and Maki Fukami
- Subjects
Genetics ,0303 health sciences ,030305 genetics & heredity ,Pseudoautosomal region ,Bisulfite sequencing ,Biology ,medicine.disease ,X-inactivation ,03 medical and health sciences ,CpG site ,DNA methylation ,medicine ,Copy-number variation ,Haploinsufficiency ,Molecular Biology ,Léri–Weill dyschondrosteosis ,Genetics (clinical) ,030304 developmental biology - Abstract
SHOX resides in the short arm pseudoautosomal region (PAR1) of the sex chromosomes and escapes X inactivation. SHOX haploinsufficiency underlies idiopathic short stature (ISS) and Leri-Weill dyschondrosteosis (LWD). A substantial percentage of cases with SHOX haploinsufficiency arise from pseudoautosomal copy number variations (CNVs) involving putative enhancer regions of SHOX. Our previous study using peripheral blood samples showed that some CpG dinucleotides adjacent to SHOX exon 1 were hypomethylated in a healthy woman and methylated in a woman with gross X chromosomal rearrangements. However, it remains unknown whether submicroscopic pseudoautosomal CNVs cause aberrant DNA methylation of SHOX-flanking CpG islands. In this study, we examined the DNA methylation status of SHOX-flanking CpG islands in 50 healthy individuals and 10 ISS/LWD patients with pseudoautosomal CNVs. In silico analysis detected 3 CpG islands within the 20-kb region from the translation start site of SHOX. Pyrosequencing and bisulfite sequencing of genomic DNA samples revealed that these CpG islands were barely methylated in peripheral blood cells and cultured chondrocytes of healthy individuals, as well as in peripheral blood cells of ISS/LWD patients with pseudoautosomal CNVs. These results, in conjunction with our previous findings, indicate that the DNA methylation status of SHOX-flanking CpG islands can be affected by gross X-chromosomal abnormalities, but not by submicroscopic CNVs in PAR1. Such CNVs likely disturb SHOX expression through DNA methylation-independent mechanisms, which need to be determined in future studies.
- Published
- 2019
31. High Performance Bulk and POU Filtration of EUV Lithography Materials
- Author
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Toru Umeda, Lucia D'Urzo, Takehito Mizuno, Amarnauth Singh, Rajan Beera, Philippe Foubert, Atsushi Hattori, and Waut Drent
- Subjects
Materials science ,POU domain ,business.industry ,Extreme ultraviolet lithography ,Blanket ,law.invention ,law ,Filter (video) ,Optoelectronics ,Wafer ,business ,Lithography ,Particle counter ,Filtration - Abstract
To comply with defect specification, Extreme Ultra-Violet (EUV) lithography necessitates performance improvement for both bulk and point-of-use (POU) filters. In this study, the data represent novel polyethylene and nylon filters for a variety of bulk and POU applications, specifically designed to: i) dramatically reduce differential pressure while achieving excellent retention, and ii) provide outstanding cleanliness for EUV grade application. Each filter was assessed by differential pressure (dP), liquid particle counter (LPC), GC-MS, and ICP-MS measurements. Finally, defect data were obtained from blanket and pattern wafers, prepared at the imec EUV cluster comprised of TEL Cleantrack LITHIUS Pro-Z and ASML NXE:3400B with a 16nm L/S test vehicle and characterized by a KLA-2935 and an eDR-7380 tools.
- Published
- 2021
32. Methylation status of genes escaping from X-chromosome inactivation in patients with X-chromosome rearrangements
- Author
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Sayaka Kawashima, Atsushi Hattori, Maki Fukami, Yukihiro Hasegawa, Kazuhiko Nakabayashi, Machiko Toki, Erina Suzuki, Masayo Kagami, Keiko Matsubara, and Rika Kosaki
- Subjects
Genetics ,Adult ,Male ,Research ,X-chromosome inactivation ,Promoter ,Methylation ,Biology ,DNA Methylation ,Escape gene ,Gene dosage ,X-inactivation ,X-chromosome rearrangement ,Genes, X-Linked ,X Chromosome Inactivation ,Reduced representation bisulfite sequencing ,Gene duplication ,Humans ,Female ,Molecular Biology ,Gene ,Genetics (clinical) ,X chromosome ,Developmental Biology - Abstract
Background X-chromosome inactivation (XCI) is a mechanism in which one of two X chromosomes in females is randomly inactivated in order to compensate for imbalance of gene dosage between sexes. However, about 15% of genes on the inactivated X chromosome (Xi) escape from XCI. The methylation level of the promoter region of the escape gene is lower than that of the inactivated genes. Dxz4 and/or Firre have critical roles for forming the three-dimensional (3D) structure of Xi. In mice, disrupting the 3D structure of Xi by deleting both Dxz4 and Firre genes led to changing of the escape genes list. To estimate the impact for escape genes by X-chromosome rearrangements, including DXZ4 and FIRRE, we examined the methylation status of escape gene promoters in patients with various X-chromosome rearrangements. Results To detect the breakpoints, we first performed array-based comparative genomic hybridization and whole-genome sequencing in four patients with X-chromosome rearrangements. Subsequently, we conducted array-based methylation analysis and reduced representation bisulfite sequencing in the four patients with X-chromosome rearrangements and controls. Of genes reported as escape genes by gene expression analysis using human hybrid cells in a previous study, 32 genes showed hypomethylation of the promoter region in both male controls and female controls. Three patients with X-chromosome rearrangements had no escape genes with abnormal methylation of the promoter region. One of four patients with the most complicated rearrangements exhibited abnormal methylation in three escape genes. Furthermore, in the patient with the deletion of the FIRRE gene and the duplication of DXZ4, most escape genes remained hypomethylated. Conclusion X-chromosome rearrangements are unlikely to affect the methylation status of the promoter regions of escape genes, except for a specific case with highly complex rearrangements, including the deletion of the FIRRE gene and the duplication of DXZ4.
- Published
- 2021
33. [Prescribing Patterns for Anti-Parkinsonian Drugs in Japan: Prescription-Based Database Study]
- Author
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Tetsuya, Maeda, Atsushi, Hattori, Junki, Yamamoto, Ken, Tsunekawa, Shigehisa, Ibe, and Futoshi, Kinoshita
- Subjects
Antiparkinson Agents ,Levodopa ,Prescriptions ,Japan ,Pharmaceutical Preparations ,Humans - Abstract
The current therapeutic approach for Parkinson's disease (PD) is mainly dopamine replacement with levodopa and other anti-parkinsonian drugs. As PD progresses, the number of these drugs used steadily increases. Using prescription-based database for 10 or more years up to October 2019, we investigated actual prescribing patterns for anti-parkinsonian drugs in Japan. The main analyses included data from patients continuously prescribed levodopa for 1 or more years (n=16,270), and of these, those continuously prescribed adjuvants to levodopa for 1 or more years (n=3,675). The results showed that the number of anti-parkinsonian drugs, their daily dose frequencies, and the number of tablets increased over time. These trends were observed not only for levodopa but also for adjuvants to levodopa; the number of adjuvants, their daily dose frequencies and number of tablets also increased. As the daily number of tablets increased, the proportion of dopamine agonists increased. Moreover, as the daily dosage of levodopa increased, the daily number of tablets increased for both overall anti-parkinsonian drugs and adjuvants to levodopa. This study revealed the process of polypharmacy in PD treatment objectively. Our results are valuable for maintaining and improving therapeutic adherence in PD. (Received 25 August, 2020; Accepted 23 October, 2020; Published 1 March, 2021).
- Published
- 2021
34. Novel functionalized membrane approaches to metal contamination control in advanced lithography filtration
- Author
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Yiren Zhang, Hassan Ait-Haddou, Atsushi Hattori, Takehito Mizuno, Rajan Beera, Frank Okezie Onyemauwa, Timothy McKeon, Belmont James A, and Toru Umeda
- Subjects
Membrane ,Materials science ,Resist ,law ,Surface modification ,Wafer ,Nanotechnology ,Photoresist ,Lithography ,Filtration ,Die (integrated circuit) ,law.invention - Abstract
As the semiconductor industry continues to advance and on-wafer defect reduction by liquid filtration has become “universal” as a process enabler for advanced technology nodes, the need for innovative filtration solutions that reduces target contaminants has become critical. Filtration technologies for metal cleanliness in photochemicals, including lithography materials and solvents, continue to grow and contribute to die yield improvement at wafer level. Both point-of-use (POU) and bulk filtration play important roles in achieving high-purity chemicals and processes by eliminating metal contaminants in critical fluids. However, the increasing complexity of photochemicals, such as multicomponent resist formulations (organic or inorganic), necessitates advanced filtration membrane technology that is compatible with their various components, including the metal oxide nanoparticles. In this regard, the ideal filtration technology should rely on functionalization with tailored chemistries to selectively remove target metals, without interactions with the key components in photoresist formulations. Also, due to the variation in forms and species of metals in different fluids, the next generation filtration technology should be able to act as a versatile platform with customization capability to optimize removal efficiency in each fluid. The objective of this study is to demonstrate the metal removal performance of different functionalization chemistries on PTFE and HDPE membrane and investigate the impact of various surface modification designs on removal selectivity and efficiency.
- Published
- 2021
35. High performance filtration for bulk materials: a novel HDPE membrane filter designed for EUV Lithography
- Author
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Takehito Mizuno, Philippe Foubert, Rajan Beera, Lucia D'Urzo, Toru Umeda, Amarnauth Singh, Waut Drent, and Atsushi Hattori
- Subjects
Materials science ,business.industry ,Extreme ultraviolet lithography ,law.invention ,Filter design ,Membrane ,Filter (video) ,law ,Optoelectronics ,Wafer ,business ,Lithography ,Particle counter ,Filtration - Abstract
Extreme Ultra-Violet (EUV) lithography is pushing material suppliers to provide the cleanest possible products for tight quality standards. The emphasis on minimizing residual particles, metals, and organics coming from materials and equipment continues to increase. Filter design and its key sub-components such as membrane continue to play a significant role to enhance performance in EUV lithography by reducing defectivity. This necessitates an improvement in retention and cleanliness for both bulk and point-of-use (POU) filters. While POU filtration targets high retention, typically achieved by membrane’s reduced pore size, the main requirement of bulk filtration is maximizing the amount of material recirculated through the filter per unit of time and is achieved with improved tortuosity and well-defined pore structure. In this study, we present a novel HDPE filter specifically designed to provide a high flow (lower differential pressure) without sacrificing retention characteristics. The new membrane was assembled in a POU filter format and compared head-to-head with a state-of-the-art HDPE membrane filter for POU application. The flow performance was assessed by differential pressure (dP) measurement, which showed an enhanced performance benefit of dP reduction by 50% compared to the reference filter, while all other test parameters are improved or at least comparable. The filter cleanliness was quantified by liquid particle counter (LPC), GC-MS, and ICP/MS measurements. Finally, comparative defect data was obtained from the blanket and pattern wafers, prepared on imec EUV cluster comprised of TEL Clean Track LITHIUS Pro-Z and ASML NXE:3400B with a 16nm L/S test vehicle.
- Published
- 2021
36. NDNF variants are rare in patients with congenital hypogonadotropic hypogonadism
- Author
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Erina Suzuki, Tsutomu Ogata, Atsushi Hattori, Satoshi Tamaoka, Maki Fukami, and Yuko Katoh-Fukui
- Subjects
Endocrine reproductive disorders ,Genetics ,Neuroendocrine diseases ,0303 health sciences ,lcsh:QH426-470 ,Genetic heterogeneity ,030305 genetics & heredity ,lcsh:Life ,Causative gene ,Rare variants ,Biology ,Biochemistry ,lcsh:Genetics ,lcsh:QH501-531 ,03 medical and health sciences ,Exon ,Data Report ,In patient ,Congenital Hypogonadotropic Hypogonadism ,Molecular Biology ,030304 developmental biology - Abstract
Although NDNF was recently reported as a novel causative gene for congenital hypogonadotropic hypogonadism (CHH), this conclusion has yet to be validated. In this study, we sequenced NDNF in 61 Japanese CHH patients. No variants, except for nine synonymous substitutions that appear to have no effect on splice-site recognition, were identified in NDNF coding exons or flanking intronic sequences. These results indicate the rarity of NDNF variants in CHH patients and highlight the genetic heterogeneity of CHH.
- Published
- 2021
37. An Automatic Image Collection System for Multicenter Clinical Studies
- Author
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Toshihiro, Takeda, Shirou, Manabe, Atsushi, Hattori, Junji, Yamaguchi, Shozo, Konishi, Yuichiro, Yamamoto, Daiyo, Takahashi, and Yasushi, Matsumura
- Subjects
Automation ,Radiology Information Systems ,Image Processing, Computer-Assisted ,Humans - Abstract
The acquisition of medical images from multiple medial institutions has become important for high-quality clinical studies. In recent years, electronic data submission has enabled the transmission of image data to independent institutions more quickly and easily than before. However, the selection, anonymization, and transmission of medical images still require human resources in the form of clinical research collaborators. In this study, we developed an image collection system that works with the electronic data capture (EDC) system. In this image collection system, medical images are selected based on EDC input information, patient ID is anonymized to a subject ID issued by the EDC, and the selected anonymized images are transferred to the research institute without human intervention. In the research institute, clinical information registered by the EDC and clinical images collected by the image collection system are managed by the same subject ID and can be used for clinical studies. In October 2019, our image collection system was introduced to 13 medical institutions and has now begun collecting medical images from the in-hospital picture archiving and communication system (PACS) of those institutions.
- Published
- 2020
38. Defectivity modulation in EUV resists through advanced filtration technologies
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Philippe Foubert, Toru Umeda, Rao Varanasi, Atsushi Hattori, Waut Drent, Jelle Vandereyken, Lucia D'Urzo, Takehito Mizuno, Rajan Beera, and Amarnauth Singh
- Subjects
Materials science ,business.industry ,Extreme ultraviolet lithography ,Surface finish ,law.invention ,Resist ,Modulation ,law ,Optoelectronics ,business ,Lithography ,Critical dimension ,Immersion lithography ,Filtration - Abstract
The availability of EUV lithography is the mainstream for resolving critical dimension of the advanced technology nodes, currently in the range of 18nm and below [1]. The first insertion of EUVL into manufacturing utilizes chemically amplified resist (CAR) [2]. The filtration of CAR, both at bulk and point-of-use (POU), has already demonstrated in ArF and ArF immersion lithography to play a significant role for microbridges reduction essentially by removing hard particle and gels [3-6]. With respect to ArFi, EUV is bringing new challenges not only for the achievement of the required line roughness, sensitivity and resolution, but also for the need of a substantial reduction of defects such as line collapse, microbridges and broken lines. In this study, it demonstrated the ability of utilizing novel POU filtration to modulate microbridges and achieving superior start-up behavior, both crucial for enabling EUVL at high volume manufacturing. Different POU filters were tested at the imec EUV cluster comprised of TEL CleanTrack LITHIUS Pro-Z and ASML NXE:3400B. The start-up performance, assessed by measuring defects down to 19nm size as a function of the flushing solvent volume, has shown the fast achievement of attaining a stable baseline. Lithography experiments targeting reduction of on-wafer defectivity, carried out with commercially available photoresists, have consistently shown a substantial reduction of after resist development (ADI) and after resist etch (AEI) microbridges on a 16nm L/S test vehicles. The effect of membrane physical intrinsic designs and novel cleaning of POU devices are discussed.
- Published
- 2020
39. The second report on spondyloepimetaphyseal dysplasia, aggrecan type: a milder phenotype than originally reported
- Author
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Osamu Miyazaki, Motomichi Kosuga, Gen Nishimura, Ryuichi Mashima, Atsushi Hattori, Dong-Kyu Jin, Sung Y. Cho, Joo-Hyun Seo, Yasuyuki Fukuhara, Torayuki Okuyama, and Maki Fukami
- Subjects
Genetics ,Male ,0303 health sciences ,Bone Diseases, Developmental ,Short Case Reports ,business.industry ,030302 biochemistry & molecular biology ,General Medicine ,Middle Aged ,Osteochondrodysplasias ,Spondyloepimetaphyseal dysplasia aggrecan type ,Phenotype ,Pathology and Forensic Medicine ,Craniofacial Abnormalities ,03 medical and health sciences ,Developmental genetics ,Pediatrics, Perinatology and Child Health ,Medicine ,Humans ,Aggrecans ,Anatomy ,business ,Genetics (clinical) ,030304 developmental biology - Published
- 2018
40. Prediction of Bending Fatigue Life of Cracked Out-of-Plane Gusset Joint Repaired by CFRP Plates
- Author
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Takafumi Komoto, Toshiyuki Ishikawa, Atsushi Hattori, Risa Matsumoto, and Hirotaka Kawano
- Subjects
Carbon fiber reinforced polymer ,Materials science ,business.industry ,Numerical analysis ,Bending fatigue ,020101 civil engineering ,02 engineering and technology ,Structural engineering ,Aspect ratio (image) ,0201 civil engineering ,020303 mechanical engineering & transports ,0203 mechanical engineering ,Solid mechanics ,Empirical formula ,business ,Joint (geology) ,Stress intensity factor ,Civil and Structural Engineering - Abstract
Carbon fiber reinforced polymer (CFRP), plates bonding repair method is one of the simple repair methods for cracked steel structures. In this study, the influence of width of CFRP plates on bending fatigue life of out-of-plane gusset joint strengthened with CFRP plates was investigated from the experimental and numerical point of view. In the bending fatigue test of cracked out-of-plane gusset joint strengthened with CFRP plates, the effect of width of CFRP plates on crack growth life was clarified experimentally. Namely, it was revealed that the crack growth life becomes larger with increasing the width of CFRP plates. In the numerical approach, the stress intensity factor (SIF) at the surface point of a semi-elliptical surface crack was estimated based on the linear fracture mechanics. Furthermore, the extended fatigue life of cracked out-of-plane gusset joint strengthened with CFRP plates was evaluated by using the estimated SIF at the surface point and the empirical formula of the aspect ratio of semi-elliptical crack. As the results of numerical analysis, the estimated fatigue life of the specimen strengthened with CFRP plates showed the good agreement with the test results.
- Published
- 2018
41. EVALUATION OF BENDING FATIGUE LIFE EXTENSION OF CRACKED OUT-OF-PLANE GUSSET JOINT REPAIRED BY PLATE BONDING
- Author
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Toshiyuki Ishikawa, Risa Matsumoto, Atsushi Hattori, Hirotaka Kawano, and Takafumi Komoto
- Subjects
Out of plane ,Materials science ,business.industry ,Bending fatigue ,Structural engineering ,business ,Joint (geology) - Published
- 2018
42. Practical use of a multicenter clinical research support system connected to electronic medical records
- Author
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Junji Yamaguchi, Yasushi Matsumura, Yoshie Shimai, Atsushi Hattori, Tomomi Yamada, Shozo Konishi, Masashi Yamamoto, Yoshiki Namiuchi, Toshihiro Takeda, and Shirou Manabe
- Subjects
Electronic data capture ,Computers ,business.industry ,Computer science ,Data management ,Medical record ,Interface (computing) ,Health Informatics ,medicine.disease ,Data structure ,Hospitals ,Computer Science Applications ,InformationSystems_GENERAL ,medicine ,Electronic Health Records ,Humans ,Data system ,Data center ,The Internet ,Medical emergency ,business ,Software ,Data Management - Abstract
Background Electronic medical records (EMRs) are widely used, but in many cases, they are used within a network physically separated from the Internet. Multicenter clinical studies use Internet-connected electronic data capture (EDC) systems to collect data, where data entered into the EMR are manually transcribed into the EDC system. In addition, medical images for clinical research are also collected manually. Variations in EMRs and differing data structures among vendors hamper the use of data for clinical research. Methods We solved this problem by developing a network infrastructure for clinical research between Osaka University Hospital and affiliated hospitals in the Osaka area and introducing a clinical data collection system (CDCS). In each hospital's EMR network, we implemented a CRF reporter that accumulated data for clinical research using a template and then sent the data to a management server in the Osaka University Hospital Data Center. To organize the patient profile data and clinical laboratory data stored in each EMR for use in clinical research, the data are retrieved from the template by an interface module developed by each vendor, according to our common data output interface specification. The data entered into the CRF reporter template for clinical research are also recorded in the EMR progress notes and sent to the data management server. This network infrastructure can also be used as a medical image collection system that automatically collects images for research from PACS at each hospital. These systems are managed under common subject numbers issued by the CDCS. Results A network infrastructure was established among 19 hospitals, and a CRF reporter was incorporated into the EMR. A medical image transfer system was introduced in 13 hospitals. Since 2013, 28 clinical studies have been conducted using this system, and data for 9,987 cases have been collected as of December 31, 2020. Conclusion Incorporating a CRF reporter with medical image transfer system into the EMR has proven useful for collecting research data.
- Published
- 2021
43. A RESEARCH ON THE EFFECT OF EVENT FOR LOOKING AROUND OF TOWN BY GAMIFICATION
- Author
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Hajime Okamoto, Shoki Toyoda, and Atsushi Hattori
- Published
- 2017
44. Next generation sequencing-based mutation screening of 86 patients with idiopathic short stature
- Author
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Yuko Katoh-Fukui, Atsushi Hattori, Marie Mitani, Shinobu Yoshida, Toshiaki Tanaka, Masanori Adachi, Akie Nakamura, Sumito Dateki, Hiroyuki Tanaka, Keisuke Nagasaki, Tsutomu Ogata, Erina Suzuki, Koji Muroya, Tsutomu Kamimaki, Kazuhiko Nakabayashi, Yoichi Matsubara, Kenichiro Hata, Keiko Matsubara, Satoshi Narumi, Maki Fukami, and Shinobu Ida
- Subjects
Male ,Receptors, Neuropeptide ,0301 basic medicine ,Endocrinology, Diabetes and Metabolism ,Growth hormone receptor ,medicine.disease_cause ,Receptor, IGF Type 1 ,Cohort Studies ,0302 clinical medicine ,Endocrinology ,Japan ,Receptors, Pituitary Hormone-Regulating Hormone ,Databases, Genetic ,STAT5 Transcription Factor ,Aggrecans ,Child ,Growth Disorders ,Genetics ,Mutation ,High-Throughput Nucleotide Sequencing ,NPR2 ,Penetrance ,Idiopathic short stature ,Child, Preschool ,Female ,medicine.symptom ,hormones, hormone substitutes, and hormone antagonists ,Heterozygote ,medicine.medical_specialty ,Expert Systems ,030209 endocrinology & metabolism ,Biology ,Short stature ,03 medical and health sciences ,Internal medicine ,medicine ,Humans ,Receptor, Fibroblast Growth Factor, Type 3 ,Genetic Predisposition to Disease ,Genetic Testing ,Gene ,Genetic Association Studies ,Glycoproteins ,Insulin-like growth factor 1 receptor ,Computational Biology ,Receptors, Somatomedin ,medicine.disease ,030104 developmental biology ,Amino Acid Substitution ,Carrier Proteins - Abstract
Although mutations in ACAN, FGFR3, NPR2, and SHOX typically lead to skeletal dysplasia, and mutations in GHRHR, GH1, GHR, STAT5B, IGF1, IGFALS, and IGF1R usually underlie hormonal defects of the growth hormone (GH)-insulin-like growth factor 1 (IGF1) axis, such mutations have also been identified in patients with idiopathic short stature (ISS). Of these, SHOX abnormalities are known to account for a certain percentage of ISS cases, whereas the frequency of mutations in the other 10 genes in ISS cohorts remains unknown. Here, we performed next-generation sequencing-based mutation screening of the 10 genes in 86 unrelated Japanese ISS patients without SHOX abnormalities. We searched for rare protein-altering variants. The functional significance of the identified variants was assessed by in silico analyses. Consequently, we identified 18 heterozygous rare variants in 19 patients, including four probable damaging variants in ACAN, six pathogenicity-unknown variants in FGFR3, GHRHR, GHR, and IGFALS, and eight possible benign variants. Pathogenic variants in NPR2, GH1, and IGF1 were absent from our cohort. Unlike previously reported patients with ACAN mutations, our four patients with ACAN variants manifested non-specific short stature with age-appropriate or mildly delayed bone ages, and had parents of normal stature. These results indicate that ACAN mutations can underlie ISS without characteristic skeletal features, and that such mutations are possibly associated with de novo occurrence or low penetrance. In addition, our data imply that mutations in FGFR3, NPR2, and GH-IGF1 axis genes play only limited roles in the etiology of ISS.
- Published
- 2017
45. DNA Methylation Status of SHOX-Flanking CpG Islands in Healthy Individuals and Short Stature Patients with Pseudoautosomal Copy Number Variations
- Author
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Kenichiro, Ogushi, Atsushi, Hattori, Erina, Suzuki, Hirohito, Shima, Masako, Izawa, Hideaki, Yagasaki, Reiko, Horikawa, Kimiaki, Uetake, Akihiro, Umezawa, Tomohiro, Ishii, Koji, Muroya, Noriyuki, Namba, Toshiaki, Tanaka, Yasuhiro, Hirano, Hitoshi, Yamamoto, Shun, Soneda, Keiko, Matsubara, Masayo, Kagami, Mami, Miyado, and Maki, Fukami
- Subjects
Adult ,Adolescent ,DNA Copy Number Variations ,Genetic Diseases, X-Linked ,Sequence Analysis, DNA ,DNA Methylation ,Osteochondrodysplasias ,Chondrocytes ,Short Stature Homeobox Protein ,Case-Control Studies ,Child, Preschool ,Humans ,CpG Islands ,Female ,Child ,Cells, Cultured ,Growth Disorders - Abstract
SHOX resides in the short arm pseudoautosomal region (PAR1) of the sex chromosomes and escapes X inactivation. SHOX haploinsufficiency underlies idiopathic short stature (ISS) and Leri-Weill dyschondrosteosis (LWD). A substantial percentage of cases with SHOX haploinsufficiency arise from pseudoautosomal copy number variations (CNVs) involving putative enhancer regions of SHOX. Our previous study using peripheral blood samples showed that some CpG dinucleotides adjacent to SHOX exon 1 were hypomethylated in a healthy woman and methylated in a woman with gross X chromosomal rearrangements. However, it remains unknown whether submicroscopic pseudoautosomal CNVs cause aberrant DNA methylation of SHOX-flanking CpG islands. In this study, we examined the DNA methylation status of SHOX-flanking CpG islands in 50 healthy individuals and 10 ISS/LWD patients with pseudoautosomal CNVs. In silico analysis detected 3 CpG islands within the 20-kb region from the translation start site of SHOX. Pyrosequencing and bisulfite sequencing of genomic DNA samples revealed that these CpG islands were barely methylated in peripheral blood cells and cultured chondrocytes of healthy individuals, as well as in peripheral blood cells of ISS/LWD patients with pseudoautosomal CNVs. These results, in conjunction with our previous findings, indicate that the DNA methylation status of SHOX-flanking CpG islands can be affected by gross X-chromosomal abnormalities, but not by submicroscopic CNVs in PAR1. Such CNVs likely disturb SHOX expression through DNA methylation-independent mechanisms, which need to be determined in future studies.
- Published
- 2018
46. Temperature-dependent magnetization reversal process and coercivity mechanism in Nd-Fe-B hot-deformed magnets.
- Author
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Satoshi Okamoto, Ryota Goto, Nobuaki Kikuchi, Osamu Kitakami, Takahiro Akiya, Hossein Sepehri-Amin, Tadakatsu Ohkubo, Kazuhiro Hono, Keiko Hioki, and Atsushi Hattori
- Subjects
TEMPERATURE ,MAGNETIZATION reversal ,COERCIVE fields (Electronics) ,MAGNETS ,NEODYMIUM ,IRON ,BORON - Abstract
Low coercivity and its large temperature dependence of a Nd
2 Fe14 B magnet with respect to its magnetic anisotropy field have been addressed as the coercivity problem. To elucidate the physical origin of this problem, we have investigated the temperature dependence of the magnetization reversal behavior in the Nd-Fe-B hot-deformed magnet. Based on the analysis of the energy barrier evaluated from magnetic viscosity measurements, the coercivity problem is discussed in terms of the following three aspects: magnetization reversal process, intrinsic coercivity without thermal demagnetization effect, and energy barrier height. The analyses lead us to conclude that domain wall pinning is dominant in the magnetization reversal in the Nd-Fe-B hot-deformed magnet. The temperature dependences of the intrinsic coercivity and the energy barrier height are explained by the grain boundary model with an intermediate layer. These analyses would be utilized to discuss the detailed structure and magnetic properties of the grain boundary, which gives a new insight to overcome the coercivity problem. [ABSTRACT FROM AUTHOR]- Published
- 2015
- Full Text
- View/download PDF
47. Encapsulation of Single Cells into Fixed Droplets Using Triangular Microwells
- Author
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Chia Wen Tsao, Tohru Yagi, and Atsushi Hattori
- Subjects
0301 basic medicine ,Materials science ,Computer Networks and Communications ,Applied Mathematics ,Microfluidics ,General Physics and Astronomy ,Nanotechnology ,02 engineering and technology ,Somewhat difficult ,021001 nanoscience & nanotechnology ,Encapsulation (networking) ,Volumetric flow rate ,03 medical and health sciences ,030104 developmental biology ,Oil phase ,Signal Processing ,Electrical and Electronic Engineering ,0210 nano-technology ,Droplet size - Abstract
Microfluidic devices using droplets make it possible to perform high-throughput screening of single-cell samples through encapsulating cells into droplets. In conventional encapsulation methods, devices have T-junction and flow-focusing geometry for droplet generation. Using those techniques, it is difficult to achieve uniform distribution of cells, or to place single cells in droplets. Moreover, in screening samples, it is necessary to have a mechanism to hold the droplets at one place for a long time. However, it is somewhat difficult to add such a function to conventional devices. Thus, we propose a new single-cell encapsulation method using triangular microwells. In this approach, each single cell is isolated, encapsulated, and held in one place by triangular microwells. This approach has the benefit that the droplet size can be regulated by the size of the microwells. In addition, optimization of encapsulation is easier than in conventional methods because the flow rate of the oil phase does not affect the encapsulation.
- Published
- 2016
48. Case Report Form Reporter: A Key Component for the Integration of Electronic Medical Records and the Electronic Data Capture System
- Author
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Yasushi, Matsumura, Atsushi, Hattori, Shiro, Manabe, Daiyo, Takahashi, Yuichiro, Yamamoto, Taizo, Murata, Akito, Nakagawa, Naoki, Mihara, and Toshihiro, Takeda
- Subjects
Clinical Studies as Topic ,Statistics as Topic ,Electronic Health Records ,Humans - Abstract
To improve the efficiency of clinical research, we developed a system to integrate electronic medical records (EMRs) and the electronic data capture system (EDC). EDC is divided into case report form (CRF) reporter and CDMS with CRF receiver with data communication using the operational data model (ODM). The CRF reporter is incorporated into the EMR to share data witth the EMR. In the data transcription type, doctors enter data using a progress note template, which are transmitted to the reporter template. It then generates the ODM. In the direct record type, reporter templates open from the progress note and generate narrative text to make record in the progress note. The configuration files for a study are delivered from the contents server to minimize the setup. This system has been used for 15 clinical studies including 3 clinical trials. This system can save labor and financial costs in clinical research.
- Published
- 2018
49. Reduction of Thermal Stress Induced in a Steel Plate Strengthened by Bonded Aluminum-CFRP Composite
- Author
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T. Nagao, Toshiyuki Ishikawa, Hirotaka Kawano, Masaru Shimizu, Atsushi Hattori, and A. Kobayashi
- Subjects
Materials science ,business.industry ,Composite number ,Alloy ,0211 other engineering and technologies ,chemistry.chemical_element ,020101 civil engineering ,02 engineering and technology ,Building and Construction ,Structural engineering ,engineering.material ,Thermal expansion ,0201 civil engineering ,Stress (mechanics) ,chemistry ,Aluminium ,021105 building & construction ,Thermal ,Bending moment ,engineering ,Adhesive ,Composite material ,business ,Civil and Structural Engineering - Abstract
In steel members strengthened with carbon-fiber reinforced polymer (CFRP) plate, thermal stresses are introduced in the steel members, the CFRP plates and the adhesive layers when temperature changes because the linear thermal expansion coefficients of steel and CFRP are mismatched. Therefore, the thermal stress caused by temperature change has to be considered when designing the repair or strengthening of a steel member with CFRP plates. With this in mind, the authors proposed a technique to reduce thermal stress in steel members strengthened by CFRP plate on both side, which involves bonding aluminum alloy plates with CFRP plates. In this proposed method, the thermal stresses in steel member can be reduced so that there are negligible levels of stress when the cross sectional areas of CFRP and aluminum plates are designed to correspond the coefficient of thermal expansion of steel, even though the thermal stresses are introduced in the CFRP and aluminum plates. So far, a thermal bending moment was not considered in the proposed method, because the steel members strengthened by CFRP-aluminum laminated plate on top and bottom sides were assumed. However, if the proposed method is applied on one side of steel member, the thermal stress in the steel member might not be reduced completely by generated thermal bending moment. Therefore, to confirm the effectiveness of the proposed method for one-sided bonding, heat tests were conducted on a steel plate with a laminated plate bonded on one side. Additionally, to verify test results and calculate the shear and peel stresses in the adhesive layers, a numerical model that used Eigenvalue analysis was proposed and applied. The tests revealed that using the proposed method to create a two-layered laminated plate consisting of CFRP and aluminum plates could not reduce the thermal stress completely in a thin steel plate. However, it was found that the thermal stress in steel plate can be completely reduced, even in thin steel plate, when the proposed method is used to create a three-layered laminated plate consisting of one CFRP plate between two aluminum plates, which when composited has a thermal bending moment equal to zero.
- Published
- 2014
50. 45,X/46,X,psu idic(Y)(q11.2) in a phenotypically normal male with short stature: a case report.
- Author
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Yasuhiro Kawabe, Mihoko Yamaguchi, Satoshi Miyagaki, Takeshi Ota, Hidechika Morimoto, Atsushi Hattori, Maki Fukami, and Jun Mori
- Subjects
SHORT stature ,HYPOSPADIAS ,Y chromosome ,CHROMOSOME analysis ,COMPARATIVE genomic hybridization ,CHROMOSOME abnormalities - Abstract
We report a case of 15-yr-old phenotypically normal male with short stature associated with the chromosomal abnormalities of 46,X,psu idic(Y)(q11.2)/45,X. At 3 yr of age, he underwent urethroplasty for scrotal hypospadias. At 15 yr of age, he was referred to our hospital due to short stature (-3.71 SD). The results of blood examination were mostly normal. A radiological examination revealed his bone age was 15.7 yr (based on the TW2-RUS method). Chromosome analysis of peripheral lymphocytes revealed 46,X,psu idic(Y)(q11.2)[16]/45,X[14], and array comparative genomic hybridization (aCGH) showed a large deletion of Yq which was located distal to the Y chromosome growth-control gene (GCY) region. It is likely that these structural abnormalities in the Y chromosome were responsible for the short stature. This case might provide new insights regarding GCY and emphasizes the importance of chromosome analysis in not only females but also males with short stature, especially when associated with genital anomalies. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
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