1. Effects of anion channel blockers on xylem nitrate transport in barley seedlings
- Author
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Hiroshi Uchida, Noriko Shigeta-Ishioka, Atsunori Tsuji, Tamikazu Kume, Chizuru Nishijo, Chizuko Mizuniwa, Shouji Hashimoto, Akihiko Osa, Tahei Kawachi, Takuji Ohyama, Hiroyuki Fujikake, T. Ito, Norikuni Ohtake, Shinpei Matsuhashi, Kuni Sueyoshi, Salwa Abdel-Latif, Toshiaki Sekine, and Satoshi Watanabe
- Subjects
biology ,Chemistry ,fungi ,food and beverages ,Soil Science ,Xylem ,Plant Science ,biology.organism_classification ,chemistry.chemical_compound ,Nitrate ,Nitrate transport ,Seedling ,DIDS ,Shoot ,Botany ,Channel blocker ,Hordeum vulgare - Abstract
The effects of anion channel blockers, 4,4′-diisothiocyanatostilbene-2,2′-disulfonic acid (DIDS) and anthracene-9-carboxylic acid (A-9-C), on the uptake and xylem transport of nitrate were investigated in intact barley (Hordeum vulgare L.) seedlings using 13NO3 − and 15NO3 − as tracers. The seedling roots were pretreated with either blocker for 1 h and then 13NO3 − or 15NO3 − was supplied to the medium. Real-time images of 13N accumulation in shoots were monitored during the first 30 min using Positron Emitting Tracer Imaging Systems (PETIS). The radioactivity in the shoots of the plants treated with either blocker was about half of that in the shoots of the control plants. Analysis of the distribution of 13N in whole seedlings using a Bioimaging Analyzer System (BAS) showed a 13N-accumulation in both shoots and roots of the control plants but reduced 13N-levels in the shoots of the plants treated with either blocker. Nitrate concentrations in the xylem sap were significantly reduced by the appli...
- Published
- 2002