1. Fine specificity analysis indicates that the primary and secondary fluorescein-specific cytotoxic T cell receptor repertoires are indistinguishable
- Author
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Wickerham C, Hughes Ea, Owen Ja, Kinnel G, and Atkeson B
- Subjects
0301 basic medicine ,Immunology ,Receptors, Antigen, T-Cell ,Biology ,Cross Reactions ,03 medical and health sciences ,chemistry.chemical_compound ,Epitopes ,Mice ,0302 clinical medicine ,Tumor Cells, Cultured ,Immunology and Allergy ,Cytotoxic T cell ,Animals ,Fluorescein ,Receptor ,Cells, Cultured ,Mice, Inbred BALB C ,Primary (chemistry) ,Cell Biology ,Fluoresceins ,Molecular biology ,Rats ,030104 developmental biology ,chemistry ,Female ,Haptens ,Immunologic Memory ,030215 immunology ,T-Lymphocytes, Cytotoxic - Abstract
Previous data from this laboratory have shown that fluorescein-specific cytotoxic T cells derived from naive mice are capable of distinguishing between different isomeric forms of the fluorescein hapten, as well as between the iodoacetamido-, isothiocyanate- and dichlorotriazinyl amino-fluorescein derivatives. In this report, we show that T cells derived from previously immunized animals, while demonstrating a stronger and more cross-reactive response overall, display identical fine specificity patterns to those of primary T cells for a panel of fluorescein homologues. We interpret this finding to indicate that the antigen receptor repertoires utilized by primary and secondary cytotoxic T cells are indistinguishable and that the enhanced response of memory as compared with naive CTL results from factors such as a higher density of cell surface receptors or receptor-associated molecules and/or a higher frequency of antigen-responsive T cells.
- Published
- 1995