43 results on '"Atencio L"'
Search Results
2. (326) Prevalence of Erectile Dysfunction in Men who Suffered from Covid-19 treated at the Centro Clinic of Barranquilla
- Author
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Martinez Preciado, J M, primary, Atencio, L, additional, Corredor, H, additional, and Sandoval, C, additional
- Published
- 2023
- Full Text
- View/download PDF
3. Circadian Modulation of Sleep-Wake Dynamics Evaluated by Transition Probabilities
- Author
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Perez-Atencio, L. F., primary, Garcia-Aracil, Nicolas, additional, Fernandez, Eduardo, additional, Barrio, Luis C., additional, and Barios, Juan A., additional
- Published
- 2017
- Full Text
- View/download PDF
4. Comparación de la educación por pares y por profesionales de la salud para mejorar el conocimiento, percepción y la conducta sexual de riesgo en adolescentes
- Author
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Sixto Sánchez C, Guillermo Atencio L, Naguye Duy, Mirtha Grande B, Maria Flores O, Marina Chiappe G, Raúl Nalvarte T, Jorge Sánchez F, and King K Holmes
- Subjects
Adolescentes ,Educación ,Grupos de Pares ,Enfermedades Sexualmente Transmisibles ,Servicios de Planificación Familiar ,Medicine ,Medicine (General) ,R5-920 - Abstract
Objetivo: Comparar la educación por pares frente a la educación por profesionales de a consultorios de planificación familiar de dos hospitales de Lima, Perú. Material y Métodos: Estudio experimental simple ciego realizado en 1998 en los consultorios de planificación familiar del Hospital Dos de Mayo y el Instituto Materno Perinatal de Lima, Perú. Después del consentimiento, se asignó aleatoriamente a 206 adolescentes consideradas de riesgo a una de las dos intervenciones educativas y luego fueron invitadas a regresar al final del tercer mes para reevaluar sus conocimientos, actitudes y conducta sexual de riesgo. Se usó la prueba de los signos, Mann Whitney y análisis de Covarianza para comparar los puntajes de los cuestionarios de conocimiento, percepción de riesgo y conducta sexual de riesgo después de la intervención educativa. Resultados: 89 (84%) de 106 adolescentes asignadas al grupo de educación por pares y 70 (70%) de las 100 asignadas al grupo de educación por profesionales de la salud (p=0,02) regresaron a la evaluación postintervención. El mejoramiento del nivel de conocimiento fue significativamente mayor (p=0,047) en el grupo de educación por pares que en el grupo capacitado por profesionales de la salud; sin embargo, esta significancia disminuyó (p=0,07) cuando se usó análisis de covarianza para controlar el puntaje obtenido antes de la intervención. El mejoramiento de la percepción y conductas de riesgo fue mayor en el grupo de pares pero esta diferencia no fue significativa. Conclusiones: La educación por pares demostró ser más efectiva en mejorar los conocimientos en las adolescentes y el seguimiento fue mejor en este grupo por lo que debe ser promovida como una estrategia para disminuir la prevalencia de infecciones de transmisión sexual en esta población.
- Published
- 2003
5. Design and preliminary results from a high temperature superconducting SQUID milliscope used for non-destructive evaluation
- Author
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Espy, M.A., Atencio, L., Flynn, E.R., Kraus, R.H., and Matlashov, A.
- Subjects
High temperature superconductors -- Design and construction ,Non-destructive testing -- Equipment and supplies ,Superconducting quantum interference devices -- Design and construction ,Business ,Electronics ,Electronics and electrical industries - Abstract
Research was conducted to examine the design and preliminary results from a superconducting quantum interference device (SQUID) milliscope. The milliscope was designed to be a nondestructive evaluation tool for stockpile stewardship as part of the Enhanced Surveillance Program at Los Alamos National Laboratory. Its goal is to identify defects before having to take the component apart. The design of the SQUID milliscope and some data that illustrate its capabilities are presented.
- Published
- 1999
6. Dose-dependent antioxidant responses and pathological changes in tenca (Tinca tinca) after acute oral exposure to Microcystis under laboratory conditions
- Author
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Atencio, L., Moreno, I., Jos, A., Pichardo, S., Moyano, R., Blanco, A., and Cameán, A. M.
- Published
- 2008
- Full Text
- View/download PDF
7. Transient Flow Assurance Analysis to Optimize Freeze Prevention Procedure in Alaska Nikaitchuq Oil Producers
- Author
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Cresta, D., additional, Hester, K. C., additional, Lullo, A. G. Di, additional, and Atencio, L., additional
- Published
- 2018
- Full Text
- View/download PDF
8. Development of SQUID microscope for localization and imaging of material defects (NDE)
- Author
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Kraus, R.H. Jr., primary, Espy, M., additional, and Atencio, L., additional
- Published
- 1997
- Full Text
- View/download PDF
9. Connexin-36 protects against suddent infant death syndrome
- Author
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Pérez Atencio, L,., Ardaiz, M., Barios Heredero, Juan Antonio, González Nieto, Daniel, and Barrio, L.C.
- Subjects
Telecomunicaciones ,Medicina - Abstract
The sudden infant death syndrome (SIDS) is the main cause of postneonatal infant death; however the etiology of SIDS remains ill defined. The hypothesis that SIDS, or a subset of SIDS, is due to an abnormal brainstem mechanism is one of the current leading hypotheses on SIDS etiology. The brainstem is essential for cardiac and respiratory function, controlling autonomic and homeostatic responses including breathing, central chemosensitivity, heartbeat and blood pressure, all mechanisms that are thought to be involved in SIDS. Connexin-36 (Cx36), the principal component of neuron-to-neuron channels that form the electrical synapses, is present in the specific neural populations of brainstem region involved in the CO2 chemoreception and respiratory control, and its expression is unregulated during this critical period of life. Accordingly, we postulate that Cx36 would be a key element in the pathogenesis of SIDS. To address this issue we have studied how the genetic suppression of Cx36 expression affects to the respiratory pacemaker, central chemoreflexes, cardiorespiratory coupling, and risk for SIDS. Mice lacking Cx36 at postnatal day 14 showed in comparison with control wild-type greater variability in the respiratory rhythmicity, abnormally enhanced ventilatory responses to hypoxia and hypercapnia, more cardiorespiratory phase synchronization and less respiratory sinus arrhythmia. A combination of low O2 and high CO2 content in inspired air was used as exogenous stressor of SIDS; under these conditions all wild-type mice survived while the 40% of Cx36-knockout animals succumbed by respiratory failure.
- Published
- 2015
10. Comparación de la educación por pares y por profesionales de la salud para mejorar el conocimiento, percepción y la conducta sexual de riesgo en adolescentes
- Author
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Sánchez C., Sixto, Atencio L., Guillermo, Duy, Naguye, Grande B., Mirtha, Flores O., María, Chiappe G., Marina, Nalvarte T., Raúl, and Holmes, King K.
- Subjects
Adolescentes ,Educación ,Grupos de Pares ,Enfermedades Sexualmente Transmisibles ,Servicios de Planificación Familiar - Abstract
Objetivo: Comparar la educación por pares frente a la educación por profesionales de a consultorios de planificación familiar de dos hospitales de Lima, Perú. Material y Métodos: Estudio experimental simple ciego realizado en 1998 en los consultorios de planificación familiar del Hospital Dos de Mayo y el Instituto Materno Perinatal de Lima, Perú. Después del consentimiento, se asignó aleatoriamente a 206 adolescentes consideradas de riesgo a una de las dos intervenciones educativas y luego fueron invitadas a regresar al final del tercer mes para reevaluar sus conocimientos, actitudes y conducta sexual de riesgo. Se usó la prueba de los signos, Mann Whitney y análisis de Covarianza para comparar los puntajes de los cuestionarios de conocimiento, percepción de riesgo y conducta sexual de riesgo después de la intervención educativa. Resultados: 89 (84%) de 106 adolescentes asignadas al grupo de educación por pares y 70 (70%) de las 100 asignadas al grupo de educación por profesionales de la salud (p=0,02) regresaron a la evaluación postintervención. El mejoramiento del nivel de conocimiento fue significativamente mayor (p=0,047) en el grupo de educación por pares que en el grupo capacitado por profesionales de la salud; sin embargo, esta significancia disminuyó (p=0,07) cuando se usó análisis de covarianza para controlar el puntaje obtenido antes de la intervención. El mejoramiento de la percepción y conductas de riesgo fue mayor en el grupo de pares pero esta diferencia no fue significativa. Conclusiones: La educación por pares demostró ser más efectiva en mejorar los conocimientos en las adolescentes y el seguimiento fue mejor en este grupo por lo que debe ser promovida como una estrategia para disminuir la prevalencia de infecciones de transmisión sexual en esta población.
- Published
- 2003
11. Pregerminative treatments on acacia San Francisco (Peltophorum pterocarpum) Fabaceae
- Author
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Atencio, L, Colmenares, R, Ramírez-Villalobos, M, and Marcano, D
- Subjects
planta ornamental ,escarification ,Germinación ,escarificación ,ornamental plant ,Germination ,Peltophorum pterocarpum - Abstract
La acacia San Francisco, es una especie de crecimiento rápido, bien adaptada a condiciones xerofíticas, y comúnmente utilizada como ornamental en la ciudad de Maracaibo (Venezuela). Se evaluó el efecto de tratamientos pregerminativos sobre el porcentaje de germinación (PG), tasa de germinación (TG) y altura de la plántula (AP). Las semillas fueron sumergidas por 24 h en ácido giberélico a 200 mg L-1 (T1), 10 min en ácido sulfúrico al 5% (T2), 10 min en agua a 80°C (T3), 24 h en agua (T4), 30 s en licuadora (T5), 20 min en lija N° 80 (T6) y un testigo sin tratamiento. El diseño experimental utilizado fue completamente aleatorizado con diez repeticiones y cinco semillas como unidad experimental. Los resultados obtenidos indican que hubo diferencias significativas (P
- Published
- 2003
12. Sequential read-out architecture for multi-channel SQUID systems
- Author
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Matlashov, A., primary, Kraus, R.H., additional, Espy, M., additional, Ruminer, P., additional, Atencio, L., additional, and Garachtchenko, A., additional
- Published
- 1999
- Full Text
- View/download PDF
13. Integrating image detector for high-energy neutrons.
- Author
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Morris, Christopher L., Armijo, V., Atencio, L. G., Bridge, A., Gavron, Avigdor, Hart, G., Morley, Kevin B., Mottershead, T., Yates, George J., and Zumbro, John
- Published
- 1997
- Full Text
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14. Elastic scattering ofπ+andπ−fromHe4between 90 and 240 MeV
- Author
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Brinkmöller, B., primary, Blilie, C. L., additional, Dehnhard, D., additional, Jones, M. K., additional, Martinez, G. M., additional, Nanda, S. K., additional, Sterbenz, S. M., additional, Yen, Yi-Fen, additional, Atencio, L. G., additional, Faucett, J. A., additional, Greene, S. J., additional, Morris, C. L., additional, Seestrom, S. J., additional, Burleson, G. R., additional, Dhuga, K. S., additional, Garnett, R. W., additional, Maeda, K., additional, Moore, C. Fred, additional, Mordechai, S., additional, Williams, A., additional, Yoo, S. H., additional, and Bland, L. C., additional
- Published
- 1991
- Full Text
- View/download PDF
15. High-count-rate proportional counter for soft x-ray position detection.
- Author
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Källne, E., Källne, J., Atencio, L. G., Chmielewski, J., Idzorek, G., and Morris, C. L.
- Subjects
PROPORTIONAL counters ,GRENZ rays - Abstract
The design of a multiwire gas proportional counter for soft x-ray detection is described. Position information is obtained over a length of 110 mm from the signal induced in the cathode plane consisting of a delay line. A position resolution of better than 80 μm is demonstrated in tests performed with fluorescent x rays of well-defined energies in the range 1.6–6 keV. Count-rate capabilities reaching 1 MHz are demonstrated in crystal spectrometer measurements of the x-ray emission from tokamak plasmas. The use of this type of detector in other applications is also discussed, especially in view of the potential of measuring the xy position over areas up to 110×160 mm2. [ABSTRACT FROM AUTHOR]
- Published
- 1987
16. Electric discharge etching of thin metalized plastic films
- Author
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Morris, C. L., Atencio, L. G., Sondheim, W. E., Seestrom, S. J., Rawool-Sullivan, M. W., McGaughey, P. L., Lee, D. M., Kinnison, W. W., Brooks, M. L., and Armijo, V.
- Published
- 1996
- Full Text
- View/download PDF
17. A successful story of reservoir management
- Author
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Vimercati, S., Raniolo, S., Paulo Gentil, Vignati, E., Giraud, H., and Atencio, L.
18. Toxic effects produced by microcystins in fish,Efectos tóxicos producidos por las microcistinas en peces
- Author
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Prieto, A. I., Atencio, L., Puerto, M., Silvia Pichardo, Jos, A., Moreno, I., and Cameán, A. M.
19. Improving separation of concerns in the development of scientific applications
- Author
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Sadjadi, S. M., Martinez, J., Soldo, T., Atencio, L., Badia, R. M., and Jorge Ejarque
20. El Grito.
- Author
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Witten, Morris, Donahue, Francis M., Pyle, David, Martínez, Dawn, Jaramillo, Cordelia, Masías, Helen, Sweeters, Susana, Philp, Thomas, García, Daniel M., Rodríguez, Juanita, C' de Baca, George, Caballero, Sharon, Cruz, Patricio, Vogel, Norma Flores, Rivera, Yolanda, Delgado, Angelina, Candelaria, Nash, Arany, Alicia, Macías, Sister Josephine, and Atencio, L. I.
- Abstract
The article presents short messages from various readers of the periodical "La Herencia" for the Summer 2004 issue. A contributor expresses his reason for subscribing the publication because it makes him feel good when he get homesick. A writer was amazed to know the Le Doux family in New Mexico because the similarity of her father's surname who also came from that place. Meanwhile, another contributor needs information regarding the genealogy of her family line in New Mexico.
- Published
- 2004
21. Large, light, high-acceptance CSC chambers for the PHENIX muon detector using a honeycomb panel design.
- Author
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Fields, D.E., Armijo, V., Atencio, L., Bassalleck, B., Behrendt, J., Brooks, M.L., Clark, D., Dinesh, B.V., Klinksiek, S., Lee, D.M., Lowe, J., Sondheim, W., Stuart, J., and Wang, X.F.
- Published
- 1997
- Full Text
- View/download PDF
22. Large CSC chamber for the PHENIX muon detector with ultra thin cathode foils.
- Author
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Lee, D.M., Armijo, V., Atencio, L., Brooks, M.L., Clark, D., Chen, Z., Dinesh, B.V., Fields, D.E., Kinnison, W.W., Hart, G., Matousek, H., Morris, C., Murray, M.M., Sondheim, W., Wang, Y., and Wang, Z.
- Published
- 1997
- Full Text
- View/download PDF
23. POETRY: The Eye of the Sky.
- Author
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Atencio, L. C.
- Subjects
- EYE of the Sky, The (Poem), ATENCIO, L. C.
- Abstract
The poem "The Eye of the Sky" by L.C. Atencio is presented. First Line: Once upon a rather odd night, a kid told me that the sky; Last Line: 'a solar eclipse.'
- Published
- 2012
24. Reduced multiple scattering using thin windows
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Atencio, L. G., Morris, C. L., and Sadler, C. P.
- Published
- 1993
- Full Text
- View/download PDF
25. Gated strip proportional detector
- Author
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Atencio, L
- Published
- 1987
26. Coronary artery calcium and cardiovascular outcomes in patients with lymphoma undergoing autologous hematopoietic cell transplantation.
- Author
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Wu S, Rhee JW, Iukuridze A, Bosworth A, Chen S, Atencio L, Manubolu V, Bhandari R, Jamal F, Mei M, Herrera A, Rodriguez F, Forman S, Nakamura R, Wong FL, Budoff M, and Armenian SH
- Subjects
- Humans, Male, Female, Middle Aged, Adult, Retrospective Studies, Aged, Young Adult, Adolescent, Coronary Vessels diagnostic imaging, Coronary Vessels pathology, Coronary Vessels metabolism, Risk Factors, Calcium metabolism, Coronary Artery Disease epidemiology, Incidence, Hematopoietic Stem Cell Transplantation adverse effects, Lymphoma therapy, Transplantation, Autologous, Cardiovascular Diseases etiology, Cardiovascular Diseases epidemiology
- Abstract
Background: Patients undergoing autologous hematopoietic cell transplantation (HCT) have a >2-fold risk of developing cardiovascular disease (CVD; heart failure, myocardial infarction, and stroke), compared to the general population. Coronary artery calcium (CAC) is predictive of CVD in nononcology patients but is not as well studied in patients who underwent HCT and survivors of HCT.The objective of this study was to examine the association between CAC and CVD risk and outcomes after HCT in patients with lymphoma., Methods: This was a retrospective cohort study of 243 consecutive patients who underwent a first autologous HCT for lymphoma between 2009 and 2014. CAC (Agatston score) was determined from chest computed tomography obtained <60 days from HCT. Multivariable Cox regression analysis was used to calculate hazard ratio (HR) estimates and 95% confidence intervals (CIs), adjusted for covariates (age, conventional risk factors [e.g., hypertension and dyslipidemia], and cancer treatment)., Results: The median age at HCT was 55.7 years (range, 18.5-75.1 years), 59% were male, and 60% were non-Hispanic White. The prevalence of CAC was 37%. The 5-year CVD incidence for the cohort was 12%, and there was an incremental increase in the incidence according to CAC score: 0 (6%), 1-100 (20%), and >100 (32%) (p = .001). CAC was significantly associated with CVD risk (HR, 3.0; 95% CI, 1.2-7.5) and worse 5-year survival (77% vs. 50%; p < .001; HR, 2.0; 95% CI, 1.1-3.4), compared to those without CAC., Conclusions: CAC is independently associated with CVD and survival after HCT. This highlights the importance of integrating readily available imaging information in risk stratification and decision-making in patients undergoing HCT, which sets the stage for strategies to optimize outcomes after HCT., (© 2024 The Authors. Cancer published by Wiley Periodicals LLC on behalf of American Cancer Society.)
- Published
- 2024
- Full Text
- View/download PDF
27. Clonal Hematopoiesis and Cardiovascular Disease in Patients With Multiple Myeloma Undergoing Hematopoietic Cell Transplant.
- Author
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Rhee JW, Pillai R, He T, Bosworth A, Chen S, Atencio L, Oganesyan A, Peng K, Guzman T, Lukas K, Sigala B, Iukuridze A, Lindenfeld L, Jamal F, Natarajan P, Goldsmith S, Krishnan A, Rosenzweig M, Wong FL, Forman SJ, and Armenian S
- Subjects
- Humans, Male, Middle Aged, Clonal Hematopoiesis, Retrospective Studies, Cardiovascular Diseases epidemiology, Cardiovascular Diseases etiology, Multiple Myeloma complications, Multiple Myeloma therapy, Hematopoietic Stem Cell Transplantation adverse effects, Coronary Artery Disease complications, Heart Failure etiology, Stroke etiology, Dyslipidemias complications
- Abstract
Importance: There is a paucity of information on the association between clonal hematopoiesis of indeterminate potential (CHIP) and cardiovascular disease (CVD) in patients with cancer, including those with multiple myeloma (MM) undergoing hematopoietic cell transplant (HCT), a population at high risk of developing CVD after HCT., Objective: To examine the association between CHIP and CVD in patients with MM and to describe modifiers of CVD risk among those with CHIP., Design, Setting, and Participants: This was a retrospective cohort study of patients with MM who underwent HCT between 2010 and 2016 at City of Hope Comprehensive Cancer Center in Duarte, California, and had pre-HCT mobilized peripheral blood stem cell (PBSC) products cryopreserved and accessible for CHIP analyses. The study team performed targeted panel DNA sequencing to detect the presence of CHIP (variant allele frequency 2% or more)., Main Outcomes and Measures: The primary end point was the 5-year cumulative incidence and risk for developing de novo CVD (heart failure, coronary artery disease, or stroke) after HCT., Results: Of 1036 consecutive patients with MM (580 male [56%]; median age, 60.0 years) who underwent a first autologous HCT, 201 patients had at least 1 CHIP variant (19.4%) and 35 patients had 2 or more variants (3.4%). The 5-year incidence of CVD was significantly higher in patients with CHIP (21.1% vs 8.4%; P < .001) compared with those without CHIP; the 5-year incidence among those with 2 or more variants was 25.6%. In the multivariable model, CHIP was associated with increased risk of CVD (hazard ratio [HR], 2.72; 95% CI, 1.70-4.39), as well as of individual outcomes of interest, including heart failure (HR, 4.02; 95% CI, 2.32-6.98), coronary artery disease (HR, 2.22; 95% CI, 1.06-4.63), and stroke (HR, 3.02; 95% CI, 1.07-8.52). Patients who had both CHIP and preexisting hypertension or dyslipidemia were at nearly 7-fold and 4-fold increased risk of CVD, respectively (reference: no CHIP, no hypertension, or dyslipidemia)., Conclusion and Relevance: CHIP was significantly and independently associated with risk of CVD in patients with MM undergoing HCT and may serve as a novel biologically plausible biomarker for CVD in this cohort. Patients with MM and both CHIP and cardiovascular risk factors had an exceptionally high risk of CVD. Additional studies are warranted to determine if cardiovascular preventive measures can reduce CHIP-associated CVD risk.
- Published
- 2024
- Full Text
- View/download PDF
28. Association Between Pretreatment Skeletal Muscle and Outcomes After CAR T-Cell Therapy.
- Author
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Lee K, Iukuridze A, He T, Bosworth A, Lindenfeld L, Teh JB, Echevarria M, Albanese S, Atencio L, Bhandari R, Wong FL, Artz AS, Siddiqi T, Nikolaenko L, Zain J, Mei M, Shouse G, Popplewell LL, Herrera AF, Budde LE, Forman SJ, and Armenian SH
- Subjects
- Humans, Male, Adolescent, Young Adult, Adult, Middle Aged, Aged, Aged, 80 and over, Female, Disease Progression, Muscle, Skeletal, Immunotherapy, Adoptive methods, Neurotoxicity Syndromes etiology
- Abstract
Background: The purpose of this study was to examine the association between baseline skeletal muscle measurements, acute toxicity (immune effector cell-associated neurotoxicity syndrome [ICANS], cytokine release syndrome), and treatment efficacy in patients undergoing CAR T-cell therapy for B-lineage lymphoma., Patients and Methods: Skeletal muscle measurements were obtained from automated CT measurements in 226 consecutive patients who received CAR T-cell therapy between 2015 and 2021. The Kaplan-Meier method was used to examine progression-free survival (PFS) and overall survival (OS) at 1-year. Multivariable regression was used to calculate the hazard ratio (HR) with 95% confidence intervals, adjusted for covariates., Results: The median age of the cohort was 63.1 years (range, 18.5-82.4 years), and most patients were male (66%) and had primary refractory disease (58%). Patients with abnormally low skeletal muscle at baseline were at greater risk of ICANS (HR, 1.74; 95% CI, 1.05-2.87) and had longer length of hospitalization (mean 27.7 vs 22.9 days; P<.05) compared with those with normal muscle mass. Abnormal skeletal muscle was independently associated with risk of disease progression (HR, 1.70; 95% CI, 1.11-2.57) and worse survival (HR, 2.44; 95% CI, 1.49-4.00) at 1 year compared with normal skeletal muscle. Individuals who had abnormal skeletal muscle and high lactate dehydrogenase (LDH) levels at baseline had poor 1-year PFS (17%) and OS (12%) compared with those with normal skeletal muscle and LDH levels (72% and 82%, respectively; P<.001). Patients who had abnormal skeletal muscle and LDH levels had a 5-fold risk (HR, 5.34; 95% CI, 2.97-9.62) of disease progression and a 10-fold risk (HR, 9.73; 95% CI, 4.81-19.70) of death (reference: normal skeletal muscle, normal LDH), independent of prior lines of therapy, extent of residual disease at time of CAR T-cell therapy, functional status, or product., Conclusions: This information can be used for risk stratification prior to CAR T-cell therapy or to implement prehabilitation and nutritional optimization before lymphodepletion as well as thereafter. These efforts will be complementary to ongoing efforts toward sustained efficacy after CAR T-cell therapy.
- Published
- 2023
- Full Text
- View/download PDF
29. Assessment of Vibrio populations in a transect of Rhizophora mangle in Punta Galeta, Panamá: culture-dependent analyses reveal biotechnological applications.
- Author
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Sánchez-Gallego J, Atencio L, Pérez J, Dupuy O, Díaz-Ferguson E, and Godoy-Vitorino F
- Abstract
Introduction: Rhizophora mangle is considered an ecological niche for microorganisms with potentially novel and complex degrading enzymes., Objective: To characterize Vibrio populations using culture-dependent methods, using samples collected from sediments and water along a red mangrove transect composed of three sites., Methods: Strains were characterized according to their distribution, capacity to degrade of organic matter and other environmental parameters. Additionally the sequence diversity was assessed using 16S rRNA sequencing., Results: Bacterial densities were strongly associated with temperature and salinity. A total of 87 good-quality sequences representing the isolates from the three sites, were binned into eight OTUs ( Operational taxonomic unit s). Taxonomic assignment indicated that the dominant members were Vibrionaceae. Beta diversity analyses showed that bacterial communities clustered by sample source rather than spatial distribution, and that alpha diversity was found to be higher in water than in sediment. Three percent of the strains from water samples could degrade carboxyl-methyl cellulose with the smallest enzymatic indexes compared to 4 % of the strains from sediment samples that showed the highest enzymatic indexes. Two strains identified as Vibrio agarivorans degraded cellulose and agarose, producing the highest enzymatic indexes., Conclusions: We found higher bacterial densities and diversity in the bacterial communities of the water samples compared to the sediment, with different OTUs including those similar to Ferrimonas, Providencia , or Shewanella which were not isolated in the sediment. Vibrio OTUs were shown to degrade cellulose in both sample types. The results of this study highlight the importance of red mangroves as Vibrio habitats and as reservoirs of potential enzyme sources with biotechnological applications.
- Published
- 2023
- Full Text
- View/download PDF
30. Association Between Body Composition and Development of Glucose Intolerance after Allogeneic Hematopoietic Cell Transplantation.
- Author
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Bhandari R, Teh JB, He T, Peng K, Iukuridze A, Atencio L, Nakamura R, Mostoufi-Moab S, McCormack S, Lee K, Wong FL, and Armenian SH
- Subjects
- Male, Female, Humans, Middle Aged, Transplantation, Homologous, Overweight, Body Composition, Obesity etiology, Retrospective Studies, Glucose Intolerance etiology, Hematopoietic Stem Cell Transplantation adverse effects
- Abstract
Background: Allogeneic hematopoietic cell transplantation (HCT) recipients have increased risk of developing glucose intolerance and diabetes mellitus (DM). The strongest risk factor for glucose intolerance is being overweight/obese, as determined by body mass index (BMI), which does not account for differences in body composition. We examined the association between body composition measures from pre-HCT CT and early-onset (≤30 days) de novo glucose intolerance after HCT, and determined its impact on nonrelapse mortality (NRM)., Methods: This study included 749 patients without pre-HCT DM. Skeletal muscle loss [abnormal skeletal muscle gauge (SMG)] and abnormal visceral adiposity (VA) were defined by sex-specific tertiles. Fine-Gray proportional subdistribution HR estimates and 95% confidence intervals (CI) were obtained to determine the association between muscle loss and VA and development of glucose intolerance. 1 year NRM was calculated for patients alive at day 30., Results: Median age at HCT was 50.2 years. By day 30, 8.1% of patients developed glucose intolerance and 731 remained alive. In multivariable analysis, abnormal SMG was associated with increased risk of glucose intolerance in nonoverweight (BMI < 25 kg/m2) patients (HR = 3.00; 95% CI, 1.15-7.81; P = 0.024); abnormal VA was associated with increased risk of glucose intolerance in overweight/obese patients (HR = 2.26; 95% CI, 1.24-4.12; P = 0.008). Glucose intolerance was independently associated with NRM (HR = 1.88; 95% CI, 1.05-3.39; P = 0.035)., Conclusions: Abnormal SMG and VA were associated with glucose intolerance in nonoverweight and overweight/obese patients, respectively, which contributed to increased risk of 1 year NRM., Impact: This information may guide personalized interventions to decrease the risk of adverse outcomes after HCT. See related commentary by Giri and Williams, p. 2002., (©2022 American Association for Cancer Research.)
- Published
- 2022
- Full Text
- View/download PDF
31. Incidence and Risk Factors for De Novo Cutaneous Squamous Cell Carcinoma in a Contemporary Cohort of Long-Term Hematopoietic Cell Transplantation Survivors.
- Author
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Berano Teh J, Abdulla F, Peng K, Atencio L, Echevarria M, Iukuridze A, Geller AC, Wong FL, Forman SJ, Nakamura R, Modi B, and Armenian SH
- Subjects
- Adult, Carcinoma, Squamous Cell immunology, Female, Follow-Up Studies, Graft vs Host Disease immunology, Graft vs Host Disease prevention & control, Humans, Immunosuppressive Agents adverse effects, Incidence, Male, Middle Aged, Retrospective Studies, Risk Factors, Skin Neoplasms immunology, Cancer Survivors statistics & numerical data, Carcinoma, Squamous Cell epidemiology, Hematopoietic Stem Cell Transplantation adverse effects, Neoplasms, Second Primary epidemiology, Skin Neoplasms epidemiology
- Published
- 2021
- Full Text
- View/download PDF
32. Conditional Survival, Cause-Specific Mortality, and Risk Factors of Late Mortality After Allogeneic Hematopoietic Cell Transplantation.
- Author
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Wong FL, Teh JB, Atencio L, Stiller T, Kim H, Chanson D, Forman SJ, Nakamura R, and Armenian SH
- Subjects
- Adolescent, Adult, Aged, California epidemiology, Child, Child, Preschool, Female, Hematopoietic Stem Cell Transplantation methods, Humans, Infant, Kaplan-Meier Estimate, Male, Middle Aged, Risk Factors, Transplantation, Homologous, Young Adult, Hematologic Diseases mortality, Hematologic Diseases therapy, Hematopoietic Stem Cell Transplantation mortality
- Abstract
Background: Long-term mortality after hematopoietic cell transplantation (HCT) is conventionally calculated from the time of HCT, ignoring temporal changes in survivors' mortality risks. Conditional survival rates, accounting for time already survived, are relevant for optimal delivery of survivorship care but have not been widely quantified. We estimated conditional survival by elapsed survival time in allogeneic HCT patients and examined cause-specific mortality., Methods: We calculated conditional survival rates and standardized mortality ratio for overall and cause-specific mortality in 4485 patients who underwent HCT for malignant hematologic diseases at a large transplant center during 1976-2014. Statistical tests were two-sided., Results: The 5-year survival rate from HCT was 48.6%. After surviving 1, 2, 5, 10, and 15 years, the subsequent 5-year survival rates were 71.2%, 78.7%, 87.4%, 93.5%, and 86.2%, respectively. The standardized mortality ratio was 30.3 (95% confidence interval [CI] = 29.2 to 35.5). Although the standardized mortality ratio declined in longer surviving patients, it was still elevated by 3.6-fold in survivors of 15 years or more (95% CI = 3.0 to 4.1). Primary disease accounted for 50% of deaths in the overall cohort and only 10% in 15-year survivors; the leading causes of nondisease-related mortality were subsequent malignancy (26.1%) and cardiopulmonary diseases (20.2%). We also identified the risk factors for nondisease-related mortality in 1- and 5-year survivors., Conclusion: Survival probability improves the longer patients survive after HCT. However, HCT recipients surviving 15 years or more remain at elevated mortality risk, largely because of health conditions other than their primary disease. Our study findings help inform preventive and interventional strategies to improve long-term outcomes after allogeneic HCT., (© The Author(s) 2020. Published by Oxford University Press. All rights reserved. For permissions, please email: journals.permissions@oup.com.)
- Published
- 2020
- Full Text
- View/download PDF
33. SGK1.1 Reduces Kainic Acid-Induced Seizure Severity and Leads to Rapid Termination of Seizures.
- Author
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Armas-Capote N, Maglio LE, Pérez-Atencio L, Martin-Batista E, Reboreda A, Barios JA, Hernandez G, Alvarez de la Rosa D, Lamas JA, Barrio LC, and Giraldez T
- Subjects
- Alternative Splicing, Animals, Electroencephalography, Excitatory Amino Acid Agonists toxicity, Hippocampus drug effects, Hippocampus physiopathology, Immediate-Early Proteins metabolism, KCNQ2 Potassium Channel metabolism, KCNQ3 Potassium Channel metabolism, Kainic Acid toxicity, Mice, Mice, Transgenic, Protein Isoforms, Protein Serine-Threonine Kinases metabolism, Seizures chemically induced, Seizures metabolism, Seizures physiopathology, Status Epilepticus chemically induced, Status Epilepticus metabolism, Status Epilepticus physiopathology, Hippocampus metabolism, Immediate-Early Proteins genetics, Neurons metabolism, Protein Serine-Threonine Kinases genetics, Seizures genetics, Status Epilepticus genetics
- Abstract
Approaches to control epilepsy, one of the most important idiopathic brain disorders, are of great importance for public health. We have previously shown that in sympathetic neurons the neuronal isoform of the serum and glucocorticoid-regulated kinase (SGK1.1) increases the M-current, a well-known target for seizure control. The effect of SGK1.1 activation on kainate-induced seizures and neuronal excitability was studied in transgenic mice that express a permanently active form of the kinase, using electroencephalogram recordings and electrophysiological measurements in hippocampal brain slices. Our results demonstrate that SGK1.1 activation leads to reduced seizure severity and lower mortality rates following status epilepticus, in an M-current-dependent manner. EEG is characterized by reduced number, shorter duration, and early termination of kainate-induced seizures in the hippocampus and cortex. Hippocampal neurons show decreased excitability associated to increased M-current, without altering basal synaptic transmission or other neuronal properties. Altogether, our results reveal a novel and selective anticonvulsant pathway that promptly terminates seizures, suggesting that SGK1.1 activation can be a potent factor to secure the brain against permanent neuronal damage associated to epilepsy., (© The Author(s) 2019. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permission@oup.com.)
- Published
- 2020
- Full Text
- View/download PDF
34. A four-state Markov model of sleep-wakefulness dynamics along light/dark cycle in mice.
- Author
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Perez-Atencio L, Garcia-Aracil N, Fernandez E, Barrio LC, and Barios JA
- Subjects
- Animals, Electroencephalography, Male, Markov Chains, Mice, Mice, Inbred C57BL, Darkness, Light, Sleep physiology, Wakefulness physiology
- Abstract
Behavioral states alternate between wakefulness (wk), rapid eye movement (rem) and non-rem (nrem) sleep at time scale of hours i.e., light and dark cycle rhythms and from several tens of minutes to seconds (i.e., brief awakenings during sleep). Using statistical analysis of bout duration, Markov chains of sleep-wk dynamics and quantitative EEG analysis, we evaluated the influence of light/dark (ld) changes on brain function along the sleep-wk cycle. Bout duration (bd) histograms and Kaplan-Meier (km) survival curves of wk showed a bimodal statistical distribution, suggesting that two types of wk do exist: brief-wk (wkb) and long-wk (wkl). Light changes modulated specifically wkl bouts, increasing its duration during active/dark period. In contrast, wkb, nrem and rem bd histograms and km curves did not change significantly along ld cycle. Hippocampal eeg of both types of wk were different: in comparison wkb showed a lower spectral power in fast gamma and fast theta bands and less emg tone. After fitting a four-states Markov chain to mice hypnograms, moreover in states transition probabilities matrix was found that: in dark/active period, state-maintenance probability of wkl increased, and probability of wkl to nrem transition decreased; the opposite was found in light period, favoring the hypothesis of the participation of brief wk into nrem-rem intrinsic sleep cycle, and the role of wkl in SWS homeostasis. In conclusion, we propose an extended Markov model of sleep using four stages (wkl, nrem, rem, wkb) as a fully adequate model accounting for both modulation of sleep-wake dynamics based on the differential regulation of long-wk (high gamma/theta) epochs during dark and light phases.
- Published
- 2018
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- View/download PDF
35. The change in antioxidant properties of dextran-coated redox active nanoparticles due to synergetic photoreduction-oxidation.
- Author
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Barkam S, Das S, Saraf S, McCormack R, Richardson D, Atencio L, Moosavifazel V, and Seal S
- Subjects
- Light, Nanotechnology, Oxidation-Reduction, Particle Size, Antioxidants chemistry, Cerium chemistry, Dextrans chemistry, Nanoparticles chemistry
- Abstract
Nanoparticles have proven to be novel material with resourceful applications in the field of nanomedicine. Cerium oxide nanoparticles (CNPs) coated with dextran (Dex-CNPs) have been shown to exhibit anticancer properties which is attributed to the change in oxidation states mediated at the oxygen vacancies on the surface of CNPs. In this study, the extreme sensitivity of Dex-CNPs to visible light is demonstrated using room light with a clear indication of synergetic phenomenon of photoreduction of CNPs in the presence of dextran which undergoes simultaneous oxidation. The phenomenon was further confirmed through a systematic time-based expedited study using a high intensity visible light source. The physiochemical changes of Dex-CNPs such as dispersion stability, pH, surface chemistry, antioxidant property, cytotoxicity and the surrounding microenvironment of Dex-CNPs were significantly altered on exposure to visible light, thereby affecting the biological response. Given the significance of nanoparticles which are widely researched nanomaterials, in different fields of nanotechnology and biomedicine, this study demonstrates the significant changes in physiochemical properties of Dex-CNPs with light. The photoreduction of Dex-CNPs affects its bifunctional applications in cancer therapy and thereby this study puts forward the necessity to preserve and sustain their properties through proper storage., (© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
- Published
- 2015
- Full Text
- View/download PDF
36. Microbiota of healthy corals are active against fungi in a light-dependent manner.
- Author
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Moree WJ, McConnell OJ, Nguyen DD, Sanchez LM, Yang YL, Zhao X, Liu WT, Boudreau PD, Srinivasan J, Atencio L, Ballesteros J, Gavilán RG, Torres-Mendoza D, Guzmán HM, Gerwick WH, Gutiérrez M, and Dorrestein PC
- Subjects
- Animals, Antifungal Agents isolation & purification, Molecular Sequence Data, Pseudoalteromonas growth & development, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Anthozoa microbiology, Antifungal Agents pharmacology, Fungi drug effects, Light, Microbiota, Pseudoalteromonas isolation & purification, Symbiosis physiology
- Abstract
Coral reefs are intricate ecosystems that harbor diverse organisms, including 25% of all marine fish. Healthy corals exhibit a complex symbiosis between coral polyps, endosymbiotic alga, and an array of microorganisms, called the coral holobiont. Secretion of specialized metabolites by coral microbiota is thought to contribute to the defense of this sessile organism against harmful biotic and abiotic factors. While few causative agents of coral diseases have been unequivocally identified, fungi have been implicated in the massive destruction of some soft corals worldwide. Because corals are nocturnal feeders, they may be more vulnerable to fungal infection at night, and we hypothesized that the coral microbiota would have the capability to enhance their defenses against fungi in the dark. A Pseudoalteromonas sp. isolated from a healthy octocoral displayed light-dependent antifungal properties when grown adjacent to Penicillium citrinum (P. citrinum) isolated from a diseased Gorgonian octocoral. Microbial MALDI-imaging mass spectrometry (IMS) coupled with molecular network analyses revealed that Pseudoalteromonas produced higher levels of antifungal polyketide alteramides in the dark than in the light. The alteramides were inactivated by light through a photoinduced intramolecular cyclization. Further NMR studies led to a revision of the stereochemical structure of the alteramides. Alteramide A exhibited antifungal properties and elicited changes in fungal metabolite distributions of mycotoxin citrinin and citrinadins. These data support the hypothesis that coral microbiota use abiotic factors such as light to regulate the production of metabolites with specialized functions to combat opportunistic pathogens at night.
- Published
- 2014
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37. Longitudinal trajectory of sexual functioning after hematopoietic cell transplantation: impact of chronic graft-versus-host disease and total body irradiation.
- Author
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Wong FL, Francisco L, Togawa K, Kim H, Bosworth A, Atencio L, Hanby C, Grant M, Kandeel F, Forman SJ, and Bhatia S
- Subjects
- Adult, Chronic Disease, Female, Graft vs Host Disease complications, Hematopoietic Stem Cell Transplantation adverse effects, Humans, Male, Prospective Studies, Regression Analysis, Risk Assessment methods, Risk Assessment statistics & numerical data, Risk Factors, Sexual Behavior radiation effects, Sexual Dysfunction, Physiological etiology, Surveys and Questionnaires, Time Factors, Whole-Body Irradiation adverse effects, Graft vs Host Disease physiopathology, Hematopoietic Stem Cell Transplantation methods, Sexual Behavior physiology, Sexual Dysfunction, Physiological physiopathology, Whole-Body Irradiation methods
- Abstract
This prospective study described the trajectory of sexual well-being from before hematopoietic cell transplantation (HCT) to 3 years after in 131 allogeneic and 146 autologous HCT recipients using Derogatis Interview for Sexual Function and Derogatis Global Sexual Satisfaction Index. Sixty-one percent of men and 37% of women were sexually active pre-HCT; the prevalence declined to 51% (P = .01) in men and increased to 48% (P = .02) in women at 3 years post-HCT. After HCT, sexual satisfaction declined in both sexes (P < .001). All sexual function domains were worse in women compared with men (P ≤ .001). Orgasm (P = .002) and drive/relationship (P < .001) declined in men, but sexual cognition/fantasy (P = .01) and sexual behavior/experience (P = .01) improved in women. Older age negatively impacted sexual function post-HCT in both sexes (P < .01). Chronic graft-versus-host disease was associated with lower sexual cognition/fantasy (P = .003) and orgasm (P = .006) in men and sexual arousal (P = .05) and sexual satisfaction (P = .005) in women. All male sexual function domains declined after total body irradiation (P < .05). This study identifies vulnerable subpopulations that could benefit from interventional strategies to improve sexual well-being.
- Published
- 2013
- Full Text
- View/download PDF
38. MS/MS networking guided analysis of molecule and gene cluster families.
- Author
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Nguyen DD, Wu CH, Moree WJ, Lamsa A, Medema MH, Zhao X, Gavilan RG, Aparicio M, Atencio L, Jackson C, Ballesteros J, Sanchez J, Watrous JD, Phelan VV, van de Wiel C, Kersten RD, Mehnaz S, De Mot R, Shank EA, Charusanti P, Nagarajan H, Duggan BM, Moore BS, Bandeira N, Palsson BØ, Pogliano K, Gutiérrez M, and Dorrestein PC
- Subjects
- Amino Acid Sequence, Bacillus genetics, Bacterial Proteins chemistry, Bacterial Proteins genetics, Molecular Sequence Data, Peptides chemistry, Peptides genetics, Pseudomonas genetics, Multigene Family, Tandem Mass Spectrometry methods
- Abstract
The ability to correlate the production of specialized metabolites to the genetic capacity of the organism that produces such molecules has become an invaluable tool in aiding the discovery of biotechnologically applicable molecules. Here, we accomplish this task by matching molecular families with gene cluster families, making these correlations to 60 microbes at one time instead of connecting one molecule to one organism at a time, such as how it is traditionally done. We can correlate these families through the use of nanospray desorption electrospray ionization MS/MS, an ambient pressure MS technique, in conjunction with MS/MS networking and peptidogenomics. We matched the molecular families of peptide natural products produced by 42 bacilli and 18 pseudomonads through the generation of amino acid sequence tags from MS/MS data of specific clusters found in the MS/MS network. These sequence tags were then linked to biosynthetic gene clusters in publicly accessible genomes, providing us with the ability to link particular molecules with the genes that produced them. As an example of its use, this approach was applied to two unsequenced Pseudoalteromonas species, leading to the discovery of the gene cluster for a molecular family, the bromoalterochromides, in the previously sequenced strain P. piscicida JCM 20779(T). The approach itself is not limited to 60 related strains, because spectral networking can be readily adopted to look at molecular family-gene cluster families of hundreds or more diverse organisms in one single MS/MS network.
- Published
- 2013
- Full Text
- View/download PDF
39. Imaging mass spectrometry of a coral microbe interaction with fungi.
- Author
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Moree WJ, Yang JY, Zhao X, Liu WT, Aparicio M, Atencio L, Ballesteros J, Sánchez J, Gavilán RG, Gutiérrez M, and Dorrestein PC
- Subjects
- Animals, Bacillus chemistry, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Symbiosis, Anthozoa microbiology, Antifungal Agents analysis, Aspergillus fumigatus physiology, Aspergillus niger physiology, Bacillus physiology
- Abstract
Fungal infections are increasing worldwide, including in the aquatic environment. Microbiota that coexist with marine life can provide protection against fungal infections by secretion of metabolites with antifungal properties. Our laboratory has developed mass spectrometric methodologies with the goal of improving our functional understanding of microbial metabolites and guiding the discovery process of anti-infective agents from natural sources. GA40, a Bacillus amyloliquefaciens strain isolated from an octocoral in Panama, displayed antifungal activity against various terrestrial and marine fungal strains. Using matrix-assisted laser desorption/ionization-imaging mass spectrometry (MALDI-IMS), the molecular species produced by this microbe were visualized in a side-by-side interaction with two representative fungal strains, Aspergillus fumigatus and Aspergillus niger. The visualization was performed directly on the agar without the need for extraction. By evaluating the spatial distributions, relative intensities and m/z values of GA40 secreted metabolites in the fungal interactions and singly grown control colonies, we obtained insight into the antifungal activity of secreted metabolites. Annotation of GA40 metabolites observed in MALDI-IMS was facilitated by MS/MS networking analysis, a mass spectrometric technique that clusters metabolites with similar MS/MS fragmentation patterns. This analysis established that the predominant GA40 metabolites belong to the iturin family. In a fungal inhibition assay of A. fumigatus, the GA40 iturin metabolites were found to be responsible for the antifungal properties of this Bacillus strain.
- Published
- 2013
- Full Text
- View/download PDF
40. Differentiation between microcystin contaminated and uncontaminated fish by determination of unconjugated MCs using an ELISA anti-Adda test based on receiver-operating characteristic curves threshold values: application to Tinca tinca from natural ponds.
- Author
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Moreno IM, Herrador MÁ, Atencio L, Puerto M, González AG, and Cameán AM
- Subjects
- Animals, Cyanobacteria classification, Cyanobacteria isolation & purification, Environmental Monitoring methods, Food Contamination analysis, Fresh Water chemistry, Fresh Water microbiology, Liver metabolism, Microcystins analysis, ROC Curve, Water Pollutants, Chemical analysis, Cyprinidae metabolism, Enzyme-Linked Immunosorbent Assay methods, Microcystins metabolism, Water Pollutants, Chemical metabolism
- Abstract
The aim of this study was to evaluate whether the enzyme-linked immunosorbent assay (ELISA) anti-Adda technique could be used to monitor free microcystins (MCs) in biological samples from fish naturally exposed to toxic cyanobacteria by using receiver operating characteristic (ROC) curve software to establish an optimal cut-off value for MCs. The cut-off value determined by ROC curve analysis in tench (Tinca tinca) exposed to MCs under laboratory conditions by ROC curve analysis was 5.90-μg MCs/kg tissue dry weight (d.w.) with a sensitivity of 93.3%. This value was applied in fish samples from natural ponds (Extremadura, Spain) in order to asses its potential MCs bioaccumulation by classifying samples as either true positive (TP), false positive (FP), true negative (TN), or false negative (FN). In this work, it has been demonstrated that toxic cyanobacteria, mainly Microcystis aeruginosa, Aphanizomenon issatchenkoi, and Anabaena spiroides, were present in two of these ponds, Barruecos de Abajo (BDown) and Barruecos de Arriba (BUp). The MCs levels were detected in waters from both ponds with an anti-MC-LR ELISA immunoassay and were of similar values (between 3.8-6.5-μg MC-LR equivalent/L in BDown pond and 4.8-6.0-μg MC-LR equivalent/L in BUp). The MCs cut-off values were applied in livers from fish collected from these two ponds using the ELISA anti-Adda technique. A total of 83% of samples from BDown pond and only 42% from BUp were TP with values of free MCs higher than 8.8-μg MCs/kg tissue (d.w.)., (Copyright © 2009 Wiley Periodicals, Inc.)
- Published
- 2011
- Full Text
- View/download PDF
41. Effects of dietary selenium on the oxidative stress and pathological changes in tilapia (Oreochromis niloticus) exposed to a microcystin-producing cyanobacterial water bloom.
- Author
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Atencio L, Moreno I, Jos A, Prieto AI, Moyano R, Blanco A, and Cameán AM
- Subjects
- Animals, Antioxidants metabolism, Cyanobacteria metabolism, Dietary Supplements, Fish Diseases chemically induced, Fish Diseases pathology, Gastrointestinal Tract drug effects, Gastrointestinal Tract pathology, Glutathione metabolism, Glutathione Disulfide metabolism, Glutathione Transferase metabolism, Kidney pathology, Lipid Peroxidation drug effects, Liver drug effects, Liver metabolism, Liver pathology, Microcystins metabolism, Myocardium pathology, Animal Feed analysis, Diet veterinary, Microcystins toxicity, Oxidative Stress drug effects, Selenium pharmacology, Tilapia
- Abstract
The present study investigates the role of selenium (Se) supplementation (as sodium selenite) on the oxidative stress and histopathological changes induced by cyanobacterial cells containing microcystins (MCs) in tilapia fish (Oreochromis niloticus). Variation in lipid peroxidation (LPO) levels and carbonyl groups content, reduced glutathione/oxidized glutathione (GSH/GSSG) ratio, and catalase (CAT), superoxide dismutase (SOD), glutathione reductase (GR), glutathione peroxidase (GPx) and glutathione S-transferase (GST) activities in liver and kidney of tilapia fish exposed to a single oral dose of 120 microg MC-LR/fish and sacrificed in 24 h, were investigated in the absence and presence of 1.5, 3.0 and 6.0 microg Se/g diet. Results showed a protective role of Se depending on the dose and the biomarker considered. Thus, the lower Se dose made CAT, liver GR and kidney SOD converged to basal values, whereas LPO and liver SOD and GST needed the higher dose. Kidney GR, however, was not protected at any Se dose. Moreover, Se has also shown to have a pro-oxidant effect with increased kidney LPO values and liver and kidney GPx activities in MC-free fish. The microscopic study revealed tissue alterations induced by cyanobacterial cells in the liver, kidney, heart and gastrointestinal tract that were ameliorated by the highest Se dose assayed. The level of Se supplementation must be therefore carefully selected to provide beneficial effects and to avoid potential negative consequences.
- Published
- 2009
- Full Text
- View/download PDF
42. Acute effects of microcystins MC-LR and MC-RR on acid and alkaline phosphatase activities and pathological changes in intraperitoneally exposed tilapia fish (Oreochromis sp.).
- Author
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Atencio L, Moreno I, Prieto AI, Moyano R, Molina AM, and Cameán AM
- Subjects
- Animals, Chemical and Drug Induced Liver Injury, Gills drug effects, Gills enzymology, Gills pathology, Heart drug effects, Injections, Intraperitoneal, Kidney drug effects, Kidney enzymology, Kidney pathology, Kidney Diseases chemically induced, Kidney Diseases enzymology, Kidney Diseases pathology, Liver drug effects, Liver enzymology, Liver pathology, Liver Diseases enzymology, Liver Diseases pathology, Myocardium pathology, Organ Size drug effects, Acid Phosphatase metabolism, Alkaline Phosphatase metabolism, Bacterial Toxins toxicity, Cichlids, Microcystins toxicity
- Abstract
Microcystins (MC) are frequently present in cyanobacterial blooms in rivers and lakes, increasing the risk of toxicity to both animals and humans. There more than eighty reported microcystins, and the present study was undertaken to determine whether MC-LR and MC-RR can induce different enzyme alterations and histopathological changes in tilapia fish (Oreochromis sp.) exposed to a single intraperitoneal (i.p.) injection of the pure standards (MC-LR and MC-RR) at a dose of 500 mug/kg; the tilapia fish were then observed for seven days. The two MC variants caused significant changes in the activities of acid and alkaline phosphatases (ACP and ALP) in vital organs, showing a different response pattern. The livers and kidneys of fish injected with MC-LR were particularly affected. MC-RR induced a very pronounced increase of ACP in the kidney and a significant increase of ALP in the liver. Both MC variants caused pathological lesions in hepatic tissues, such as megalocytosis, necrotic process, and microvesicular steatosis, particularly in fish treated with MC-LR, and degenerative renal changes, glomerulopathy, were more severe in tilapias exposed to MC-RR. In addition, both microcystins also caused significant myopathy in the heart. In contrast, the gills did not show any change in enzyme activity or histopathological injury.
- Published
- 2008
- Full Text
- View/download PDF
43. [Mechanism of vagal reflex arterial hypertension].
- Author
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CICARDO VH, MAGGIOLO ATENCIO L, and NAVA ACEVEDO H
- Subjects
- Hypertension, Reflex, Vagus Nerve physiology
- Published
- 1958
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