1. In vitro schistosomicidal and antiviral activities of Arctium lappa L. (Asteraceae) against Schistosoma mansoni and Herpes simplex virus-1.
- Author
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Dias MM, Zuza O, Riani LR, de Faria Pinto P, Pinto PLS, Silva MP, de Moraes J, Ataíde ACZ, de Oliveira Silva F, Cecílio AB, and Da Silva Filho AA
- Subjects
- Animals, Cell Death drug effects, Cell Survival drug effects, Chlorocebus aethiops, Chromatography, High Pressure Liquid, Male, Plant Extracts chemistry, Reproduction drug effects, Spectrometry, Mass, Electrospray Ionization, Vero Cells, Antiviral Agents pharmacology, Arctium chemistry, Herpesvirus 1, Human drug effects, Plant Extracts pharmacology, Schistosoma mansoni drug effects, Schistosomicides pharmacology
- Abstract
Schistosomiasis and herpes diseases represent serious issues to the healthcare systems, infecting a large number of people worldwide, mainly in developing countries. Arctium lappa L. (Asteraceae), known as "bardana" and "burdock", is a medicinal plant popularly used for several purposes, including as antiseptic. In this study, we evaluated the in vitro schistosomicidal and antiherpes activities of the crude extract of A. lappa, which have not yet been described. Fruits of A. lappa L. were extracted by maceration with ethanol: H
2 O (96:4 v/v) in order to obtain the hydroalcoholic extract of A. lappa (AL). In vitro schistosomicidal assays were assessed against adult worms of Schistosoma mansoni, while the in vitro antiviral activity of AL was evaluated on replication of Herpes simplex virus type-1 (HSV-1). Cell viability was measured by MTT assay, using Vero cells and chemical composition of AL was determined by qualitative UPLC-ESI-QTOF-MS analysis. UPLC-ESI-QTOF-MS analysis of AL revealed the presence of dibenzylbutyrolactone lignans, such as arctiin and arctigenin. Results showed that AL was not cytotoxic to Vero cells even when tested at 400μg/mL. qPCR results indicated a significant viral load decreased for all tested concentrations of AL (400, 50, and 3.125μg/mL), which showed similar antiviral effect to acyclovir (50μg/mL) when tested at 400μg/mL. Also, AL (400, 200, and 100μg/mL) caused 100% mortality and significantly reduction on motor activity of all adult worms of S. mansoni. Confocal laser scanning microscopy showed tegumental morphological alterations and changes on the numbers of tubercles of S. mansoni worms in a dose-dependent manner after treatment with AL. This report provides the first evidence for the in vitro schistosomicidal and antiherpes activities of AL, opening the route to further schistosomicidal and antiviral studies with AL and their compounds, especially lignans., (Copyright © 2017 Elsevier Masson SAS. All rights reserved.)- Published
- 2017
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