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1. Pooling saliva samples as an excellent option to increase the surveillance for SARS-CoV-2 when re-opening community settings.

Author

Joaquín Moreno-Contreras, Marco A Espinoza, Carlos Sandoval-Jaime, Marco A Cantú-Cuevas, Daniel A Madrid-González, Héctor Barón-Olivares, Oscar D Ortiz-Orozco, Asunción V Muñoz-Rangel, Cecilia Guzmán-Rodríguez, Manuel Hernández-de la Cruz, César M Eroza-Osorio, Carlos F Arias, and Susana López

Subjects
Medicine, Science
Abstract

In many countries a second wave of infections caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has occurred, triggering a shortage of reagents needed for diagnosis and compromising the capacity of laboratory testing. There is an urgent need to develop methods to accelerate the diagnostic procedures. Pooling samples represents a strategy to overcome the shortage of reagents, since several samples can be tested using one reaction, significantly increasing the number and speed with which tests can be carried out. We have reported the feasibility to use a direct lysis procedure of saliva as source for RNA to SARS-CoV-2 genome detection by reverse transcription quantitative-PCR (RT-qPCR). Here, we show that the direct lysis of saliva pools, of either five or ten samples, does not compromise the detection of viral RNA. In addition, it is a sensitive, fast, and inexpensive method that can be used for massive screening, especially considering the proximity of the reincorporation of activities in universities, offices, and schools.

Published
2022
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2. Saliva Sampling and Its Direct Lysis, an Excellent Option To Increase the Number of SARS-CoV-2 Diagnostic Tests in Settings with Supply Shortages

Author

Marco A Espinoza, Manuel Hernández-de la Cruz, César M Eroza-Osorio, Asunción V. Muñoz-Rangel, Carlos F. Arias, Oscar Ortiz-Orozco, Marco A Cantú-Cuevas, Carlos Sandoval-Jaime, Susana López, Héctor Barón-Olivares, and Joaquín Moreno-Contreras

Subjects
0301 basic medicine, Microbiology (medical), Saliva, Lysis, viruses, Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), 030106 microbiology, Oropharynx, Economic shortage, Genome, Viral, Real-Time Polymerase Chain Reaction, Virus, Specimen Handling, 03 medical and health sciences, Betacoronavirus, 0302 clinical medicine, COVID-19 Testing, Nasopharynx, Medicine, Humans, Viral rna, 030212 general & internal medicine, business.industry, Clinical Laboratory Techniques, Diagnostic Tests, Routine, SARS-CoV-2, Diagnostic test, Viral Load, Virology, RNA, Viral, business, Coronavirus Infections, Viral load
Abstract

As part of any plan to lift or ease the confinement restrictions that are in place in many different countries, there is an urgent need to increase the capacity of laboratory testing for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Detection of the viral genome through reverse transcription-quantitative PCR (RT-qPCR) is the gold standard for this virus; however, the high demand of the materials and reagents needed to sample individuals, purify the viral RNA, and perform the RT-qPCR has resulted in a worldwide shortage of several of these supplies. Here, we show that directly lysed saliva samples can serve as a suitable source for viral RNA detection that is less expensive and can be as efficient as the classical protocol, which involves column purification of the viral RNA. In addition, it bypasses the need for swab sampling, decreases the risk of the health care personnel involved in the testing process, and accelerates the diagnostic procedure.

Published
2020
Full Text
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3. Saliva sampling is an excellent option to increase the number of SARS CoV2 diagnostic tests in settings with supply shortages

Author

Asunción V. Muñoz-Rangel, Oscar Ortiz-Orozco, Carlos Sandoval-Jaime, Marco A Cantú-Cuevas, Carlos F. Arias, Héctor Barón-Olivares, Marco A Espinoza, Joaquín Moreno-Contreras, Susana López, Manuel Hernández-de la Cuz, and César M Eroza-Osorio

Subjects
Saliva, Risk analysis (engineering), Computer science, Diagnostic test, Economic shortage, Viral rna, Laboratory testing
Abstract

As part of any plan to lift or ease the confinement restrictions that are in place in many different countries, there is an urgent need to increase the capacity of laboratory testing for SARS CoV-2. Detection of the viral genome through RT-qPCR is the golden standard for this test, however, the high demand of the materials and reagents needed to sample individuals, purify the viral RNA, and perform the RT-qPCR test has resulted in a worldwide shortage of several of these supplies. Here, we show that directly lysed saliva samples can serve as a suitable source for viral RNA detection that is cheaper and can be as efficient as the classical protocol that involves column purification of the viral RNA. In addition, it surpasses the need for swab sampling, decreases the risk of the healthcare personnel involved in this process, and accelerates the diagnostic procedure.

Published
2020

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