19 results on '"Astakhova NM"'
Search Results
2. Analysis of the Basic Characteristics of Osteogenic and Chondrogenic Cell Lines Important for Tissue Engineering Implants.
- Author
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Astakhova NM, Korel' AV, Shchelkunova EI, Orishchenko KE, Nikolaev SV, Zubairova US, and Kirilova IA
- Subjects
- Alkaline Phosphatase genetics, Alkaline Phosphatase metabolism, Animals, Biomarkers metabolism, Bone and Bones metabolism, Calcium metabolism, Cartilage metabolism, Cell Differentiation, Cell Line, Cell Movement, Cell Size, Chondrocytes metabolism, Collagen Type II genetics, Collagen Type II metabolism, Gene Expression, Mitotic Index, Osteoblasts metabolism, Swine, Swine, Miniature, Bone and Bones cytology, Cartilage cytology, Chondrocytes cytology, Chondrogenesis genetics, Osteoblasts cytology, Osteogenesis genetics
- Abstract
We isolated and characterized cultures of bone and cartilage tissue cells of laboratory minipigs. The size and morphological features of adherent osteogenic and chondrogenic cells were specified. During long-term culturing under standard conditions, the studied cultures expressed specific markers that were detected by immunohistochemical staining: alkaline phosphatase and calcium deposits in osteoblasts and type II collagen and cartilage extracellular matrix in chondrogenic cells. Proliferative potential (mitotic index) of both cell types was 4.64% of the total cell number. Cell motility, i.e. the mean velocity of cell motion was 49 pixels/h for osteoblasts and 47 pixels/h for chondroblasts; the mean migration distance was 2045 and 2118 pixels for chondroblasts and osteoblasts, respectively. The obtained cell lines are now used as the control for evaluation of optimal biocompatibility of scaffold materials in various models. Characteristics of the motility of the bone and cartilage tissue cells can be used for modeling and estimation of the rate of cells population of 3D scaffolds made of synthetic and biological polymers with different internal structure and physicochemical properties during designing in vitro tissue implants.
- Published
- 2018
- Full Text
- View/download PDF
3. Host genetic risk factors for West Nile virus infection and disease progression.
- Author
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Bigham AW, Buckingham KJ, Husain S, Emond MJ, Bofferding KM, Gildersleeve H, Rutherford A, Astakhova NM, Perelygin AA, Busch MP, Murray KO, Sejvar JJ, Green S, Kriesel J, Brinton MA, and Bamshad M
- Subjects
- 2',5'-Oligoadenylate Synthetase genetics, Female, GTP-Binding Proteins genetics, Haplotypes genetics, Humans, Interferon Regulatory Factor-3 genetics, Male, Middle Aged, Myxovirus Resistance Proteins, Polymorphism, Single Nucleotide genetics, West Nile Fever pathology, Genetic Predisposition to Disease genetics, West Nile Fever epidemiology, West Nile Fever genetics
- Abstract
West Nile virus (WNV), a category B pathogen endemic in parts of Africa, Asia and Europe, emerged in North America in 1999, and spread rapidly across the continental U.S. Outcomes of infection with WNV range from asymptomatic to severe neuroinvasive disease manifested as encephalitis, paralysis, and/or death. Neuroinvasive WNV disease occurs in less than one percent of cases, and although host genetic factors are thought to influence risk for symptomatic disease, the identity of these factors remains largely unknown. We tested 360 common haplotype tagging and/or functional SNPs in 86 genes that encode key regulators of immune function in 753 individuals infected with WNV including: 422 symptomatic WNV cases and 331 cases with asymptomatic infections. After applying a Bonferroni correction for multiple tests and controlling for population stratification, SNPs in IRF3 (OR 0.54, p = 0.035) and MX1, (OR 0.19, p = 0.014) were associated with symptomatic WNV infection and a single SNP in OAS1 (OR 9.79, p = 0.003) was associated with increased risk for West Nile encephalitis and paralysis (WNE/P). Together, these results suggest that genetic variation in the interferon response pathway is associated with both risk for symptomatic WNV infection and WNV disease progression.
- Published
- 2011
- Full Text
- View/download PDF
4. Characterization of equine and other vertebrate TLR3, TLR7, and TLR8 genes.
- Author
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Astakhova NM, Perelygin AA, Zharkikh AA, Lear TL, Coleman SJ, MacLeod JN, and Brinton MA
- Subjects
- Alleles, Animals, Base Sequence, Cats, Cattle, Conserved Sequence, DNA, Complementary genetics, Dogs, Evolution, Molecular, Exons, Gene Frequency, Horses genetics, Immunogenetic Phenomena, Introns, Molecular Sequence Data, Phylogeny, Polymorphism, Single Nucleotide, Protein Structure, Tertiary, RNA, Messenger genetics, Rabbits, Species Specificity, Toll-Like Receptor 3 chemistry, Toll-Like Receptor 7 chemistry, Toll-Like Receptor 8 chemistry, Horses immunology, Toll-Like Receptor 3 genetics, Toll-Like Receptor 7 genetics, Toll-Like Receptor 8 genetics, Vertebrates genetics, Vertebrates immunology
- Abstract
Toll-like receptors 3, 7, and 8 (TLR3, TLR7, and TLR8) were studied in the genomes of the domestic horse and several other mammals. The messenger RNA sequences and exon/intron structures of these TLR genes were determined. An equine bacterial artificial chromosome clone containing the TLR3 gene was assigned by fluorescent in situ hybridization to the horse chromosomal location ECA27q16-q17 and this map location was confirmed using an equine radiation hybrid panel. Direct sequencing revealed 13 single-nucleotide polymorphisms in the coding regions of the equine TLR 3, 7, and 8 genes. Of these polymorphisms, 12 were not previously reported. The allelic frequency was estimated for each single-nucleotide polymorphism from genotyping data obtained for 154 animals from five horse breeds. Some of these frequencies varied significantly among different horse breeds. Domain architecture predictions for the three equine TLR protein sequences revealed several conserved regions within the variable leucine-rich repeats between the corresponding horse and cattle TLR proteins. A phylogenetic analysis did not indicate that any significant exchanges had occurred between paralogous TLR7 and TLR8 genes in 20 vertebrate species analyzed.
- Published
- 2009
- Full Text
- View/download PDF
5. Concerted evolution of vertebrate CCR2 and CCR5 genes and the origin of a recombinant equine CCR5/2 gene.
- Author
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Perelygin AA, Zharkikh AA, Astakhova NM, Lear TL, and Brinton MA
- Subjects
- Animals, Base Sequence, Cats, Cattle, Chickens, Chromosome Mapping, DNA, Elephants, Equidae classification, Exons, Genotype, Humans, In Situ Hybridization, Fluorescence, Introns, Molecular Sequence Data, Phylogeny, Polymorphism, Single Nucleotide, Rabbits, Swine, Synteny, Equidae genetics, Evolution, Molecular, Receptors, CCR2 genetics, Receptors, CCR5 genetics, Recombination, Genetic, Vertebrates genetics
- Abstract
Chemokine receptors (CCRs) play an essential role in the initiation of an innate immune host response. Several of these receptors have been shown to modulate the outcome of viral infections. The recent availability of complete genome sequences from a number of species provides a unique opportunity to analyze the evolution of the CCR genes. A phylogenetic analysis revealed that the CCR2 gene evolved in concert with the paralogous CCR5 gene, but not with another paralogous gene, CCR3, in the opossum, platypus, rabbit, guinea pig, cat, and rodent lineages. In addition, evidence of concerted evolution of the CCR2 and CCR5 genes was observed in chicken and lizard genomes. A unique CCR5/2 gene that originated by unequal crossing over between the CCR2 and CCR5 genes was detected in the domestic horse. The CCR2, CCR5, and CCR5/2 genes were mapped to ECA16q21 using fluorescent in situ hybridization (FISH). Single-nucleotide polymorphisms identified in the equine CCR5 gene and characterized within 5 horse breeds provide haplotype markers for future case/control studies investigating the genetic bases of horse susceptibility to infectious diseases.
- Published
- 2008
- Full Text
- View/download PDF
6. Comparative mapping of mink chromosome 8p: in situ hybridization of seven cattle BAC clones.
- Author
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Larkin DM, Prokhorovich MA, Astakhova NM, and Zhdanova NS
- Subjects
- Animals, Cattle, Chromosome Mapping, Chromosomes, Artificial, Bacterial, Humans, Mice, Synteny, In Situ Hybridization, Fluorescence, Mink genetics
- Published
- 2006
- Full Text
- View/download PDF
7. Comparative mapping of cattle chromosome 19: cytogenetic localization of 19 BAC clones.
- Author
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Larkin DM, Astakhova NM, Prokhorovich MA, Lewin HA, and Zhdanova NS
- Subjects
- Animals, Chromosomes, Artificial, Bacterial, Cloning, Molecular, Computational Biology, Evolution, Molecular, Humans, In Situ Hybridization, Fluorescence, Mice, Sequence Homology, Nucleic Acid, Cattle genetics, Chromosome Mapping
- Abstract
Here we present the results of fluorescent in situ hybridization (FISH) mapping of a set of cattle BAC clones preselected for assignment on cattle chromosome 19 (BTA19). The BAC clones were anchored to human chromosome 17 (HSA17) sequences by BLASTn similarity search of cattle BAC-ends against the human genome sequence (NCBI build 33). Five blocks of homologous synteny were defined in the comparative map of BTA19 and HSA17 built with FISH data and the human genome coordinates. The positions for four evolutionary breakpoints in the bovine and human chromosomes were identified. Comparison of the FISH comparative map with previously published comparative RH, physical, and cytogenetic maps of BTA19 did not reveal major conflicts and allowed for the extension of the boundaries of homology between BTA19 and HSA17. Comparative analysis of HSA17, BTA19, and mouse chromosome 11 (MMU11) demonstrates that most likely mice retain the ancestral organization of the synteny group, and both cattle and human chromosomes underwent several major internal rearrangements after the divergence of Primates, Rodentia, and Cetartiodactyla., (2006 S. Karger AG, Basel.)
- Published
- 2006
- Full Text
- View/download PDF
8. Unusual distribution pattern of telomeric repeats in the shrews Sorex araneus and Sorex granarius.
- Author
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Zhdanova NS, Karamisheva TV, Minina J, Astakhova NM, Lansdorp P, Kammori M, Rubtsov NB, and Searle JB
- Subjects
- Animals, Cells, Cultured, Chromosomes, In Situ Hybridization, Fluorescence, Shrews genetics, Telomere genetics
- Abstract
Sorex araneus and Sorex granarius are sibling species within the Sorex araneus group with karyotypes composed of almost identical chromosome arms. S. granarius has a largely acrocentric karyotype, while, in S. araneus, various of these acrocentrics have combined together by Robertsonian (Rb) fusions to form metacentrics, with the numbers and types of metacentrics differing between chromosomal races. Our studies on telomeric sequences in S. araneus and S. granarius revealed differences between chromosomes and between species. In S. araneus (the Novosibirsk race), hybridization signals were present on the telomeres of all the chromosomes after FISH with a PCR-generated telomeric probe. In addition, hybridization signals were observed at high frequencies in the pericentric regions of some but not all metacentrics formed by Rb fusion. There were fewer signals on those metacentrics formed earlier in the evolution of S. araneus. This suggests that S. araneus chromosomes retain at least some telomeric repeats during Rb fusion, but that these repeats are lost or modified over time. These results are critical for the interpretation of the well-studied hybrid zones between chromosomal races of S. araneus, given that Rb fission has been postulated in such hybrid zones and that the likelihood of Rb fission will relate to presence/absence of telomeric sequences at the centromeres of metacentrics. In S. granarius, there were strong signals at the proximal (centromeric) telomeres of the acrocentrics after FISH with a DNA telomeric probe. FISH with a PNA telomeric probe on S. granarius acrocentrics showed that the proximal telomeres were 213 kb on average, while the length of the distal telomeres was 3.8 kb on average. Two-colour FISH, using a telomeric DNA probe and a microdissected probe generated from the pericentric regions of the S. granarius chromosomes a and b, revealed regions on distinct chromatin fibres where telomeric and microdissected probes were colocalized or localized sequentially. The proximal telomeres of S. granarius are highly unusual both in their large size and their heterogeneous structure relative to the telomeres of other mammals.
- Published
- 2005
- Full Text
- View/download PDF
9. Simple and efficient solid support scavenging of excess acyl donors after enzymatic acylations in organic solvents.
- Author
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Usyatinsky AY, Astakhova NM, and Khmelnitsky YL
- Subjects
- Acylation, Enzyme Activation, Enzymes, Immobilized chemistry, Fungal Proteins, Organic Chemicals chemistry, Polystyrenes chemistry, Silica Gel, Silicon Dioxide chemistry, Substrate Specificity, Free Radical Scavengers chemistry, Lipase chemistry, Solvents chemistry, Vinyl Compounds chemistry
- Abstract
A simple and efficient method for removing excess acyl donors following enzymatic acylations in organic solvents was developed. This method is based on selective chemical scavenging of acyl donors using an amino-functionalized solid support, and does not affect the desired acylated product. A wide variety of different acyl donors, including vinyl and trifluoroethyl esters and vinyl carbonates, can be quantitatively removed by this method, thus providing a simple and highly efficient tool for purification of reaction products after enzymatic acylation., (Copyright 2003 Wiley Periodicals, Inc. Biotechnol Bioeng 82: 379-385, 2003.)
- Published
- 2003
- Full Text
- View/download PDF
10. [Use of PCR markers for mapping swine chromosome 12].
- Author
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Larkin DM, Kuznetsov SB, Astakhova NM, and Zhdanova NS
- Subjects
- Animals, Base Sequence, Cricetinae, DNA Primers, Hybrid Cells, Mink genetics, Polymerase Chain Reaction, Chromosome Mapping veterinary, Genetic Markers, Swine genetics
- Abstract
Using PCR analysis of pig-mink and pig-Chinese hamster hybrid cell lines and heterologous and homologous primers of various types, chromosomal and subchromosomal mapping of genes TOP2A, THRA, BRCA1, GAS, HLR1, MYL4, LIS1, MCP1, ENO3, CRYB1, P4HB, STAT5B, and H3F3B to pig chromosome 12 was carried out. The efficiency of using different types of heterologous primers for pig chromosome mapping was compared.
- Published
- 2001
11. Zoo-FISH with region-specific paints for mink chromosome 5q: delineation of inter- and intrachromosomal rearrangements in human, pig, and fox.
- Author
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Rubtsov NB, Karamisheva TV, Astakhova NM, Liehr T, Claussen U, and Zhdanova NS
- Subjects
- Animals, Chromosomes, Human, Pair 17 genetics, Conserved Sequence genetics, DNA Probes genetics, Evolution, Molecular, Humans, Sequence Homology, Nucleic Acid, Shrews genetics, Chromosome Painting methods, Chromosomes genetics, Foxes genetics, Mink genetics, Recombination, Genetic genetics, Swine genetics
- Abstract
Comparison of evolutionarily conserved mammalian chromosomes homologous to human chromosome 17, performed with microdissected painting probes, revealed rearrangements inside these chromosomes in mink and pig and a disruption of this conserved region in the fox. Detection of a homologous region on an Iberian shrew chromosome showed the efficiency of microdissected painting probes for delineation of homologous chromosome regions in species belonging to orders that diverged at least 100 million years ago., (Copyright 2000 S. Karger AG, Basel.)
- Published
- 2000
- Full Text
- View/download PDF
12. [Chromosome localization and analysis of synteny analysis of some genes in swine, cattle, and sheep (Artiodactyla)].
- Author
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Kuznetsov SB, Larkin DM, Kaftanovskaia EM, Ivanova EV, Astakhova NM, Cheriakene OV, and Zhdanova NS
- Subjects
- Animals, Genetic Markers, Humans, Hybrid Cells, Mink genetics, Biological Evolution, Cattle genetics, Chromosome Mapping, Sheep genetics, Swine genetics
- Abstract
Using the hybrid cell lines pig-American mink, cow-American mink, and sheep-American mink, the localization of some genes included in a large conservative block localized on human chromosome (chr) 17 was performed by means of electrophoresis of proteins and Southern blot hybridization. Genes NF1, RARA, PRKCA, and ERBB2 were assigned to chr 12 in swine; TK1 and UMPH2, to chr 19 in cattle; and TK1, UMPH2, and PEPA, to chr 11 in sheep. The conserved synteny of these genes in three representatives of the order Artiodactyla was shown.
- Published
- 1998
13. [A new approach to the analysis of complex chromosomal rearrangements in cell hybrids].
- Author
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Rubtsov NB, Pliusnina EV, Serdiukova NA, Astakhova NM, Biltueva LS, Kuznetsov SB, Grafodatskiĭ AS, and Zhdanova NS
- Subjects
- Animals, Clone Cells, Gene Library, In Situ Hybridization, Fluorescence, Karyotyping, Metaphase genetics, Mink, Swine, Chromosomes, Gene Rearrangement, Hybrid Cells physiology
- Abstract
The chromosomal complements of somatic cell pig-mink hybrids was determined by a new approach. This approach includes microdissection of metaphase chromosomes, generation of chromosome and region-specific DNA libraries, and fluorescence in situ hybridization of these libraries with pig lymphocyte chromosomes. The studied hybrid cells were shown to contain two small acrocentric chromosomes and a microchromosome of porcine origin. Identification of these chromosomes by differential GTG-staining was impossible. Chromosome isolation by a micromanipulation technique followed by DNA amplification in TOPO-DOP polymerase chain reaction provided chromosome-specific DNA libraries of the rearranged chromosomes. Based on these libraries, the labeled DNA probes were prepared and hybridized to pig chromosomes. This allowed us to determine the origin of the material contributing to the hybrid cell chromosomes. One of these chromosomes contained five pig chromosomal regions: 15cen-q2; 6q21-q23; 13q21; 13q22; 7q25-qter, while the other contained the following pig chromosomal regions: 4p12-p13; 16q12-q14; 12pter-p15. The microchromosome contained the Xp11-Xq11 region. The minimal size of the revealed chromosomal regions was about 3 to 4 x 10(6) bp. Segregation analysis of the thymidine kinase gene 1 (TK1), which was earlier localized to the pig 12p region, and the hybrid cell pig chromosomes in the hybrid subclones suggested that TK1 gene can be assigned to 12p15-pter. The results obtained demonstrate the efficiency of the applied approach in its detailed and reliable description of complex chromosomal rearrangements in hybrid clones, when differential chromosome staining failed to identify these chromosomes.
- Published
- 1998
14. Production of pig-mink cell hybrids with single pig chromosomes 2, 5, 12, or t(1,13).
- Author
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Zhdanova NS, Thomsen PD, Astakhova NM, Kuznetsov SB, Jörgensen CB, Plyusnina EV, and Serov OL
- Subjects
- Animals, Chromosome Banding, Clone Cells, Genetic Markers, Hybrid Cells, Karyotyping, L-Lactate Dehydrogenase genetics, Mink, Polymerase Chain Reaction, Thymidine Kinase genetics, Chromosome Mapping, Swine genetics
- Published
- 1996
- Full Text
- View/download PDF
15. Characterization of pig-mink cell hybrids: assignment of the TK1 and UMPH2 genes to pig chromosome 12.
- Author
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Zhdanova NS, Astakhova NM, Kuznetsov SB, Schuler L, and Serov OL
- Subjects
- 5'-Nucleotidase metabolism, Animals, Cell Line, Cloning, Molecular, Fibroblasts cytology, Fibroblasts enzymology, Hybrid Cells, Isoenzymes metabolism, Lymphocytes cytology, Lymphocytes enzymology, Male, Mink, Mutagenesis, Insertional, Phosphates metabolism, Swine, Thymidine Kinase metabolism, 5'-Nucleotidase genetics, Chromosome Mapping methods, Isoenzymes genetics, Thymidine Kinase genetics
- Abstract
By fusion of thymidine kinase-deficient mink cells with pig leukocytes, a new type of cell hybrid was produced. It was demonstrated that pig chromosomes segregate in pig-mink hybrids and that hybrid cells contain no cytologically visible rearrangements between the chromosomes of parental species, or chromosome fragmentation. With a set of subclones of two primary hybrid clones, the genes for thymidine kinase-1 (TK1) and uridine 5'-monophosphate hydrolase-2 (UMPH2) were assigned to pig Chromosome (Chr) 12. A cell line with a single pig Chr 8 on the background of mink chromosomes was established. This clone could serve as a source of DNA for building a chromosome-specific library of pig Chr 8. The data obtained suggest that pig-mink cell hybrids can be used for mapping of pig chromosomes.
- Published
- 1994
- Full Text
- View/download PDF
16. [Preliminary localization of TK1 and UMPH2 genes on swine chromosome 12].
- Author
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Astakhova NM, Zhdanova NS, Kaftanovsiaia EM, Kuznetsov SB, and Serov OL
- Subjects
- Animals, Hybrid Cells, Karyotyping, Male, Mink, Swine, Chromosome Mapping
- Abstract
A new type of pig-mink cell hybrids was produced. It was demonstrated that segregation of pig chromosomes occurs in these hybrids and that no rearrangements between the chromosomes of different species occurs; fragmentation of pig chromosomes is also absent. Using these hybrids, genes TK1 and UMPH2 were preliminarily located on pig chromosome 12. The obtained data demonstrate that these hybrids can be used for chromosome mapping of pig genes.
- Published
- 1994
17. [A line of Chinese hamster cells containing human chromosome 18].
- Author
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Zhdanova NS, Pak SD, Astakhova NM, Matiakhina LD, and Serov OL
- Subjects
- Animals, Cricetinae, Humans, Hybrid Cells physiology, In Situ Hybridization, CHO Cells physiology, Chromosomes, Human, Pair 18
- Published
- 1992
18. [Mapping of the locus for the H blood-group system on the 15th chromosome of domestic swine].
- Author
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Tikhonov VN, Gorelov IG, Nikitin SV, Bobovich VE, and Astakhova NM
- Subjects
- Animals, Crosses, Genetic, Female, Genotype, Heterozygote, Male, Recombination, Genetic, Blood Group Antigens genetics, Chromosome Mapping, Genetic Markers, Swine blood
- Published
- 1983
19. [Detailed analysis of a new translocation in a domestic pig].
- Author
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Astakhova NM, Vysotskaia LV, and Grafodatskiĭ AS
- Subjects
- Animals, Chromosome Banding, Male, Swine genetics, Translocation, Genetic
- Abstract
A sterile minisibs (mini Siberian swine) boar with normal sexual behaviour was studied by the routine, C- and high resolution G-banding chromosome techniques. A new reciprocal translocation was identified involving chromosomes 12 and 13. The numbers of diakinesis and sperms were very low. The synaptonemal complex was typical of reciprocal translocations.
- Published
- 1988
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