108 results on '"Assefa F"'
Search Results
2. Review on enhancing the efficiency of fertilizer utilization: Strategies for optimal nutrient management
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Nakachew Kelemu, Yigermal Habtamu, Assefa Fenta, Gelaye Yohannes, and Ali Solomon
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fertilizer use efficiency ,nutrient management ,nutrient stewardship ,precision agriculture ,sustainable agriculture ,Agriculture ,Agriculture (General) ,S1-972 - Abstract
The increasing global population and growing demand for food and mineral fertilizers underscore the urgent need to enhance fertilizer efficiency. This imperative emphasizes the importance of sustainable fertilizer utilization while mitigating environmental impacts, particularly in addressing agricultural water pollution. Excessive fertilizer use contributes significantly to water contamination and food shortages worldwide. In 2018, food shortages were reported in many nations, including the United States (2.3%), Canada (4.6%), the United Kingdom (8.2%), Germany (2.6%), Japan (2.9%), Ethiopia (23.4%), Ivory Coast (22.4%), Bangladesh (12.7%), Pakistan (17.2%), Haiti (45.6%), and India (14.3%). Moreover, agricultural activities, particularly the use of mineral fertilizers, are major contributors to greenhouse gas emissions. Inefficient fertilizer practices lead to economic losses, environmental degradation, and food insecurity. Studies reveal that in sub-Saharan Africa, farmers receive only about $0.50 in increased productivity for every dollar spent on fertilizer due to inefficiencies. The economic cost of nutrient pollution in the European Union is estimated to range between €7 billion and €10 billion annually. Effective strategies like precision nutrient management, best practices, and innovative technologies optimize fertilizer efficiency and support agricultural sustainability. Besides, promising methods include the combined use of organic and inorganic fertilizers, the application of remote sensing and geographical information system technologies, and the implementation of biological approaches to enhance nutrient management. Moreover, monitoring and evaluation are essential for assessing strategy effectiveness, guiding decision-making, and taking corrective actions. Hence, this review aims to address strategies for improving fertilizer efficiency, sustainable agriculture practices, and addressing food security and environmental concerns related to fertilizer use comprehensively.
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- 2024
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3. The Effects of Plant Growth-Promoting Bacteria (PGPB) Inoculation on Growth, Yield, and Grain Nutrient Uptake of Two Teff Varieties Under Greenhouse Condition
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Feleke A, Alemu T, Assefa F, Bekele D, Chaniyalew S, and Tsegaye Z
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Plant growth ,Nutrient ,Agronomy ,Inoculation ,Yield (chemistry) ,Greenhouse ,food and beverages ,anatomy_morphology ,Biology ,biology.organism_classification ,Bacteria - Abstract
Application of native PGPR as bio inoculant is an alternative sustainable agricultural practice to enhance crop productivity, grain quality, and soil fertility. In this view, a study was to examine the effect of either individual or consortium PGPR inoculation on growth, yield, and grain nutrient uptake of two teff varieties. The pot experiment was carried out in (CRD) three replication and 10 treatments. The PGPR inoculants used in this study were Pseudomonas fluorescens biotype G, Enterobacter cloacae ss disolvens, and Serratia marcescens ss marcescen and their consortium. Dukem and Magna varieties were used in this study. The results of the analysis of variance showed significant differences (P ≤ 0.001) among the treatment and most of the agronomic traits except number of fertile tillers and also significant different (P ≤ 0.01) for grain P and N uptake. The variety was significantly affected grain Mg, Zn and Fe uptake at 5 % probability level and did not significantly influence all agronomic traits of the two varieties. Furthermore, interaction effects of two factors (TM*VT) were significant differences (P ≤ 0.05) for plant height and panicle length. Individual treatments mean comparison results showed that inoculation of native PGPR consortium significantly affected most of the PGP traits at (P ≤ 0.05). The maximum traits like plant height (189cm), panicle length (66.7cm), shoot dry biomass (9.98g), root dry biomass (2.90g) and grain yield per plant (4.55g) were observed from Dz-01-196. It could be concluded that the consortium of native PGPR inoculants for plant growth, yield and grain nutrient uptake improvement performed better than their individual strain.
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- 2021
4. Developing small-scale bamboo enterprises for livelihoods and environmental restoration in Benishangul-Gumuz Regional State, Ethiopia
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Boissière, Manuel, Atmadja, Stibniati Soeria, Benmakhlouf, S., Beyessa, M., Kassa, Habtemariam, Hunde, T., Assefa, F., Boissière, Manuel, Atmadja, Stibniati Soeria, Benmakhlouf, S., Beyessa, M., Kassa, Habtemariam, Hunde, T., and Assefa, F.
- Abstract
We document the perceptions, practices and policy options in managing lowland bamboo [Oxytenanthera abyssinica] in Benishangul Gumuz Regional State (BGRS) in Ethiopia, particularly to enable small-scale enterprises (SSE) to become more active in this field. This region hosts the largest extent of natural bamboo forests in Ethiopia. There is a recent push to realize bamboo's economic and environmental potential in Ethiopia, which puts SSEs as crucial actors. There is little or scattered published information on local perceptions and practices surrounding natural bamboo forest management and options for realizing bamboo's potential from a subnational/local perspective in Africa, including in Ethiopia. In 2018, we conducted a literature review, spatial analysis, participatory mapping, and interviewed experts working in governmental and non-governmental organizations and local stakeholders in BGRS. We find some consensus of the environmental importance of bamboo. In contrast, there is some debate at the subnational level about the economic importance of bamboo, leading to decisions favouring other land uses. Bamboo forests in this region suffer neglect, as they are perceived to be 'owned by no one and used by everyone' and will continue to be there without management. Lack of market-driven opportunities, bamboo-specific training among foresters, data on economic contributions of bamboo, and regulations or guidelines to support existing laws have prevented effective management of the bamboo resource. There are multiple bamboo management approaches that open economic opportunities for SSEs in the region. There needs to be more clarity on how to secure land use rights over bamboo forests, accessible financing, market linkages, business training, and low-tech/low-cost technologies to encourage the development of bamboo SSEs.
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- 2020
5. Biological nitrogen and organic matter removal from tannery wastewater in pilot plant operations in Ethiopia
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Leta, S., Assefa, F., Gumaelius, L., and Dalhammar, G.
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- 2004
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6. Identification of Efficient Denitrifying Bacteria from Tannery Wastewaters in Ethiopia and a Study of the Effects of Chromium III and Sulphide on Their Denitrification Rate
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Leta, S., Gumaelius, L., Assefa, F., and Dalhammar, G.
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- 2004
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7. Developing small-scale bamboo enterprises for livelihoods and environmental restoration in Benishangul-Gumuz Regional State, Ethiopia
- Author
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Boissière, M., primary, Atmadja, S., additional, Benmakhlouf, S., additional, Beyessa, M., additional, Kassa, H., additional, Hunde, T., additional, and Assefa, F., additional
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- 2020
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8. Nodulation pattern and acetylene reduction (nitrogen fixation) activity of some highland and lowland Acacia species of Ethiopia
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Assefa, F. and Kleiner, D.
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- 1998
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9. Exploratory regression modeling for flood susceptibility mapping in the GIS environment
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Wang Fenglin, Imran Ahmad, Martina Zelenakova, Assefa Fenta, Mithas Ahmad Dar, Afera Halefom Teka, Amanuel Zewdu Belew, Minwagaw Damtie, Marshet Berhan, and Sebahadin Nasir Shafi
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Medicine ,Science - Abstract
Abstract Understanding the temporal and spatial patterns of flood in the Awash River basin, which is located in Ethiopia’s Afar region, is crucial. The Awash basin was picked because it is continuously in danger both spatially and temporally. The likelihood of flooding was assessed using eight independent variables: elevation, slope, rainfall, drainage density, land use, soil type, wetness index, and lineament density. Each constituent was assigned a weight based on its susceptibility to the danger, which was classified into four classifications. Exploratory regression analysis showed that the existing land use is the main factor influencing flood susceptibility. For the GIS domain, a total of 31 models were built using exploratory regression. Model number 31 was found to be the best fit model, having the highest Adjusted R2 value of 0.8 and the lowest Akaike’s Information criterion value of 1536.8. The spatial autocorrelation tool’s Z score and p-value for the standard residuals are, respectively, 0.7 and 0.4, indicating that they were neither clustered nor scattered. The geographic breadth of flood susceptibility and risk is thoroughly examined in this paper, as is the significance of spatial planning in the Awash basin.
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- 2023
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10. Rhizospheric bacterial isolates of grass pea (Lathyrus sativusL.) endowed with multiple plant growth promoting traits
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Mussa, A., primary, Million, T., additional, and Assefa, F., additional
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- 2018
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11. Nodulation of African yam bean (Sphenostylis stenocarpa) by Bradyrhizobium sp. isolated from Erythrina brucei
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Assefa, F. and Kleiner, D.
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- 1997
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12. Rhizospheric bacterial isolates of grass pea (Lathyrus sativus L.) endowed with multiple plant growth promoting traits.
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Assefa, F., Mussa, A., and Million, T.
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RHIZOBACTERIA , *LATHYRUS sativus , *PLANT growth , *RIBOSOMAL RNA , *EFFECT of stress on bacteria , *PLANT diseases - Abstract
Aim: The main aim of this work was to examine the plant growth promoting, biocontrol activities and stress competency of grass pea rhizobacterial strains from Ethiopia. Methods and Results: Serial dilutions were carried out to isolate rhizobacterial strains from the rhizosphere soil samples. The isolates were characterized for their plant growth promoting, biocontrolling and stress tolerance potential. The isolates that showed better performance in the tested parameters were identified by 16S rRNA gene sequencing. Among the isolates tested on Pikovskaya agar medium, 22 isolates that showed solubilization index of >2·41 cm were selected for further screening tests. Isolate AAUGPR‐53 identified as Enterococcus species, Enterococcus casseliflavus strain showed the highest phosphate solubilization index and indole‐3‐acetic acid production efficiency of 4·81 ± 0·02 (μg ml−1) and 56·55 ± 0·45 (μg ml−1), respectively. Sixteen (72·7%) of the isolates showed in vitro antifungal inhibition against Fusarium oxysporum f. sp. lentis with isolates AAUGPR‐92 and AAUGPR‐91 identified as Enterococcus species, E. casseliflavus strain and Enterococcus gallinarum strain exhibiting the highest inhibition of 83 and 78%, respectively. Likewise, 68·2%, 91·30%, 45·5%, 77·3% and 100% of the isolates produced chitinase, protease, cellulase, HCN and NH3, respectively. Most of the isolates showed good tolerance to the tested stress factors. The 16S rRNA partial sequencing of the rhizobacterial isolates proved their taxonomic position in the existing bacterial isolates. Conclusion: The results indicated that three strains, AAUGPR‐53, 91 and 92, that showed maximum sequence identity (99%) to Enterococcus species, E. casseliflavus and E. gallinarum were recommended as microbial inoculants for trials under greenhouse and field conditions. Significance and Impact of the Study: This study illustrates an effective alternative to conventional fertilizers that may contribute to crop disease reduction. Our results provide a foundation for future research that will lead to identifying potentially useful biocontrol strains found in the rhizosphere of grass pea. [ABSTRACT FROM AUTHOR]
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- 2018
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13. Characterization of Phosphate Solubilizing Faba Bean (Vicia faba L.) Nodulating Rhizobia Isolated from Acidic Soils of Wollega, Ethiopia
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Kenasa, G, Jida, M, and Assefa, F
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Faba bean Rhizobial isolates Phosphate solubilization Symbiotic effectiveness - Abstract
Some species of rhizobial bacteria nodulating faba bean are characterized by phosphate solubilization. In order to study their in vitro and symbiotic characteristics, twelve rhizobial isolates nodulating faba bean were collected from acidic soil of Wollega, Ethiopia. Solubilization index of the isolates ranges from 1.25 to 2.10. Mean Generation Time of the isolates were less than 2.34; and growth of isolates on Yeast Extract Mannitol agar with bromothymoleblue media were accompanied by change of color from blue to yellow. AUAVR-51 and AUAVR-52 were the highest in TCP-Solubilizion Index. The two isolates were also recognized as stress tolerant when tested in vitro for extreme temperature, osmotic, acidic pH, intrinsic antibiotics, and acidic-aluminum as compared to the other isolates. However, there was no unique metabolic diversity and specialization of AUAVR-51 and AUAVR-52 isolates with respect to carbon and nitrogen source utilization. On the other hand, isolates AUAVR-51 and AUAVR-52 were characterized by effective and highly effective symbiosis on sterile potted sand growth, respectively. In general, phosphate solubilizing rhizobia nodulating faba bean from acidic soil are fast grower; and their solubilization potential varies. However, the contribution of these isolates as double fertilizer should be tested in the real acidic soil characterized by immobilized phosphorous.Keywords: Faba bean Rhizobial isolates Phosphate solubilization Symbiotic effectiveness
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- 2014
14. Effects of Acidity on Growth and Symbiotic Performance of Rhizobium leguminosarum bv. viciae Strains Isolated from Faba Bean Producing Areas of Ethiopia
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Jida, M and Assefa, F
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Acidity tolerance ,Nodulation ,Nitrogen fixation ,Vicia faba ,Rhizobium ,Faba bean - Abstract
Faba bean is one of the legume crops commonly grown in Ethiopia. It is important source of dietary protein to the majority of population in the country. Soil acidity and related stresses are among the major yield limiting constraints for this crop. This study was conducted with the aim of evaluating acidity tolerance of Rhizobium leguminosarum bv. viciae strains isolated from faba bean growing regions of the country and their symbiotic performance under different acidic conditions. Four strains isolated from root nodules of faba bean were tested for tolerance to acidity in a defined liquid media. The results indicated that none of the tested strains was tolerant to pH 4.0 while two of them (AUFR46 and AUFR100) were found to be tolerant of pH 4.5. When tested at pH 5.0 only one isolate (AUFR58) was sensitive. The results of the present study also showed that all acid tolerant strains were recovered from highly acidic soil (4.8- 5.2) and the acid sensitive strain was isolated from neutral soil. A positive correlation (r= 0.92) was observed between minimum pH tolerated in culture media and pH of origin soil of the strains. Nodulation and nitrogen fixation abilities of these strains were evaluated on sand culture. The results indicated that at pH 4.5 and 5.0 nodulation was totally inhibited and only one isolate (AUFR58) could not be able to induce nodule formation on host plant roots at pH 5.5. The results also showed that acidity (pH 5.5) reduced shoot dry weight, nodules number, total nitrogen at a highly significant level (P
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- 2014
15. Genetic Gain for Adzuki Bean Beetle (Callosobruchus chinensis L.) Resistance in Ethiopian Chickpea (Cicer arietinum L.) Genotypes
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Keneni, G, Bekele, E, Getu, E, Imtiaz, M, Dagne, K, and Assefa, F
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Chickpea (Cicer arietinum L.) is attacked by a number of insect pests, including the adzuki bean beetle (Callosobruchus chinensis L.). Genetic gains from selection to infestation by adzuki bean beetle were studied using 130 chickpea genotypes in 2009/2010. The test was conducted under ambient condition in the Entomology Laboratory of Holetta, Ambo and Debre Zeit, using RCBD in 3 replications. Data on infestation and seed damage levels were recorded and subjected to statistical analysis. Differences among the genotypes were mostly significant. Seed related traits generally exhibited larger heritable variations than insect related traits. The t-test revealed effective selection for almost all traits. Broad sense heritability varied from 43-76% and 0.20-11.00% for seed and insect related traits, respectively. The corresponding expected genetic gain ranged from 28-42% and 0.01-6.00% in the same order. Significant positive correlations were found among seed weight loss and three component characters, i.e. number of eggs, adults emerged and seed size. Number of uninfested seeds (%) and percent seed coat weight showed strong negative correlations with seed weight loss. Selection of genotypes could improve resistance to the beetle in this gene pool, but with a compromise for seed quality. A search for more suitable sources of variability and selection criteria would be advisable with the adoption of molecular plant breeding tools for better selection efficiency and effectiveness.Keywords: Broad Sense Heritability; Chickpea; Correlation Coefficient; Genetic Gain
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- 2014
16. Federalism and Security
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Assefa Fiseha
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Special police Ethiopia ,ethno federation ,peace and security ,regional police ,Law ,Political science - Abstract
Ensuring security is one of the defining characteristics and central claims of a state. In federal countries there is a possibility for two or more actors at different levels to have some mandate over security. In the case of federal Ethiopia the security sector remains complicated. The constituent units have established special police in addition to the regular police, and its role and status remains contested. What is the constitutional basis of the special police? Do regional states have a mandate to establish such a police? Is it a force for stability or instability? How should it be regulated? These are the main questions that this article aims to investigate based on qualitative method, relevant comparative study, and empirical investigation into this rather complex sector. The findings show that the special police resemble more an army than a police force. It has also taken over the mandate of the army, which is a federal mandate. Lately, its size has increased significantly and retired army generals have begun to join it, hinting that the army is being drained. The fact that the impact of the army is depleting owing to the ongoing civil war in the country and the constituent unit special police is slowly taking over mandate implies the center is losing control and the country is slowly falling apart along ethno- national fault lines. The article further explains the race behind the special police and proposes options on how to regulate it.
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- 2022
17. Symbiotic and phenotypic diversity of Rhizobium leguminosarum bv. viciae from Northern Gondar, Ethiopia
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Belay, Z and Assefa, F
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Faba bean, Northern Gondar, Rhizobium, phenotypic diversity, symbiotic effectiveness - Abstract
Rhizobia that nodulate cool season legumes are widely spread in the Mediterranean and highland agro ecosystems. Faba bean is one of these important crops that represent the major protein source of human nutrition, cash crop for farmers and component of the farming systems. This study is aimed at identifying rhizobia isolates capable of guarantying efficient nitrogen derived from biological nitrogen fixation. Due to this, a total of twenty-one isolates of Rhizobium were isolated from as many sampling sites of northern Gondar using plant infection method. The isolates were characterized morphologically and physiologically and tested on sand and soils to evaluate their symbiotic effectiveness. Studies on symbiotic effectiveness on sand culture indicated that, the strains showed shoot dry matter ranging from 0.4 (AUFR127) to 2.3 g/plant (AUFR124), with negative control (0.2 g/plant) and positive control of 2.4 g/plant. Eighty percent of the isolates were found to be effective and have very effective nitrogen fixers based on their shoot dry matter accumulation (50 to 100%) in relation to the nitrogen fertilized control plants. Culturally, almost all of them displayed large colonies with diameters of 2 to 4.5 mm, generation time of 1.9 to 4.3 h and showed characteristics of fast growing rhizobia. With a few exceptions, isolates grew at temperatures of 15 and 35°C and were found to be sensitive to salt, except AUFR118 that grew up to 5% NaCl. The isolates also grew on a wide range of moderate acidity and alkalinity (pH5.5-9).With the exception of gluconate, citrate and tartarate, almost all isolates grew on all carbohydrates. The pattern of intrinsic antibiotic resistance showed that, almost all of them were tolerant to chloramphenicol, nalidixic acid and erythromycin. Isolates AUFR118, AUFR128 and AUFR132 were found to be resistant to almost all tested antibiotics. These isolates were also included in the effective and very effective symbiotic groups. The trial of the 5 selected effective isolates on two soil types showed that, they performed well without any significant difference on their respective soils. Interestingly, the isolates were found to nodulate the host on the highly acidic soil (pH 4.8) that failed to be nodulated by the indigenous rhizobia. Generally, the present work shows the physiological and symbiotic diversity of the isolates in the traditional agricultural areas of the region and the potential of these rhizobia to be used as effective commercial inoculants in areas where the indigenous rhizobia fail to do so. This will help to reclaim acidic soils for faba bean production.Key words: Faba bean, Northern Gondar, Rhizobium, phenotypic diversity, symbiotic effectiveness
- Published
- 2013
18. Symbiotic and phenotypic characterization of Rhizobium isolates of field pea (Pisum sativum L.) Fabaceae, from central and southern Ethiopia
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Amsalu, A, Assefa, F, and Hailemariam, A
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Antibiotic resistance, Eco-physiological tolerance, Heavy metal resistance, Symbiotic effectiveness - Abstract
A total of 25 rhizobial isolates from field pea (Pisum sativum L.) were collected from Shewa, Gojam, Gondar, Wollo and Tigray, and authenticated as root nodule using the trap host. They showed significant variations in their symbiotic effectiveness in nitrogen fixation and enhancing growth of the pea cultivar Markose plants compared to the uninoculated and non-fertilized control plants on sand culture under greenhouse conditions. Although there was inter-site difference in harbouring effective and very effective isolates ranging from 86% (Tigray) and 67% (Gondar) in terms of shoot dry matter accumulation of 50-100%, on the average 76% of the pea rhizobial isolates in the soils of major growing areas in the country were effective and very effective. The inoculated plants also showed differences in total nitrogen contents in the range of 1.72% and 2.93%. These isolates were then characterized based on their cultural, physiological and ecological features in relation to their symbiotic characteristics. The isolates showed diversity in their eco-physiological tolerance (NaCl, pH and temperature) inherent resistance to antibiotics and heavy metals, and heterotrophic competence of utilization of 17 carbon sources. Consequently, isolates NSRlFP1, NSRlFP3, NSRlFP13, NSRlFP17 and NSRlFP18 were the elite rhizobia that can be selected and further tested for their genetic and symbiotic performance in field trials for future bio-inoculant formulation.Key words/phrases: Antibiotic resistance, Eco-physiological tolerance, Heavy metal resistance, Symbiotic effectiveness.
- Published
- 2013
19. Socioeconomic benefits of shade trees in coffee production systems in Bonga and Yayuhurumu districts, southwestern Ethiopia: Farmers’ perceptions
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Muleta, D, Assefa, F, Nemomissa, S, and Granhall, U
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Coffea Arabica is extensively cultivated by households under a variety of shade trees in southwestern Ethiopia. The main purpose of this study was to assess the overall farmers' perception on the benefits of shade trees in coffee production systems in southwestern part of Ethiopia. Semistructured questionnaires were administered to 100 smallscale coffee farmers. In-depth interviews were also made with 10 selected farmers from Bonga and Yayu-Hurumu districts study sites. Farmers’ perspectives were mostly comparable to the documented scientific facts with some noticeable differences. Among shade tree species legumes such as Albizia gummifera, Acacia abyssinica, Millettia ferruginea were highly favoured in that order. A significant number of the study subjects expressed an interest in the further propagation of the seedlings of the most favoured shade trees such as Albizia gummifera (95%), Acacia abyssinica (65%), Millettia ferruginea (55%) and Cordia africana (50%). The respondents strongly stated the serious problems associated with growing coffee without shade tree plants that included stunted growth which ultimately resulted in coffee yield reduction (97.3%) and quick wilting of coffee plants (93.6%). The majority of the respondents hassled other benefits of coffee shade trees such as firewood (96.4%) and honey production (92.7%) followed by improvement of soil fertility (79.1%) and reduction of soil erosion (78.2%). A significant number of farmers (39.1%) expressed their long experience of retaining legumes like Desmodium species in their coffee plots during weeding or clearing. Higher return values and better coffee attributes were generally assigned to shaded coffee systems particularly those dominated by tree legumes. The respondents had excellent knowledge on socioeconomic benefits of shade tree species. However, organic training is believed to minimize knowledge gaps on certain complex and/or unobservable ecosystem processes in the shaded coffee systems to boost the confidence of the farmers in supplying green commodities of premium prices on sustainable basis.Keywords: intercropping; leguminous plants; organic farming; shaded coffee systems
- Published
- 2012
20. Diversity and Plant Growth Promoting Proerties of Rhizobacteria Isolated from tef (Eragrostis tef)
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Woyessa, D and Assefa, F
- Abstract
The purpose of this study was to evaluate and assess the plant growth promoting characteristics and diversity of major tef rhizosphere isolates from central Ethiopia. A total of 162 bacteria were isolated from rhizosphere of tef [Eragrostis tef (Zucc.) Trotter] and characterized. While screening using some plant growth promoting characteristics, only about sixty six of the isolates were found to show some characteristics of plant growth promoting rhizobacteria (PGPR) and hence selected for further study. The sixty six isolates were further characterized by several morphological, physiological and biochemical tests and identified to species level using API kit method. Numerical analysis of phenotypic data by a computer cluster analysis using UPGMA (NTSYSpc software version 2.1) was done based on carbohydrate fermentation profile. The isolates displayed diverse morphological, physiological, biochemical and PGPR characteristics [(Phosphate solubilization (52%), antmicrobial property (41%) and HCN production (17%)] in addition to tolerance to a wide range of pH by most of the isolates. The 66 isolates were found to belong to 7 genera and 15 species; two species with each of two sub-species. Analysis of phenotypic data revealed that at boundary levels of 50% average similarity, two major clusters were formed by both Gram-negative and positive isolates while the respective groups form six and seven clusters at about 75% and 78.4% average similarity. The fact that the tef rhizosphere bacteria to be positive for various PGPR characteristics suggests that the isolates have better potential for greenhouse and field testing and application in improving yield of tef.Key words/phrases: API kit, Bacteria, Ethiopia, P solubilization, Screening of PGPR
- Published
- 2012
21. Response of chickpea to varying moisture stress conditions in Ethiopia
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Lijalem Korbu, Asnake Fikre, Kassahun Tesfaye, Assefa Funga, Dagnachew Bekele, and Chris O. Ojiewo
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Agriculture ,Environmental sciences ,GE1-350 - Abstract
Abstract Chickpea (Cicer arietinum L.) is an economically important crop grown by nearly one million Ethiopian smallholder farmers. The crop is often considered as “stress‐loving,” but moisture stress at flowering and grain filling stages could be detrimental. Yield of chickpea is commonly affected by terminal drought stress in the rainfed production system in Ethiopia. The lack of proper field‐screening methods has hindered the development of drought‐tolerant varieties. This study demonstrates a simple and practical field‐level screening method for drought tolerance traits in the conventional breeding programs. A field experiment was conducted using 28 elite chickpea cultivars during the 2018–2019 main cropping season to study their response to moisture regimes of varying drought intensities. We used yield and its components as proxy parameters of screening to select tolerant cultivars. The study revealed significant variation among the cultivars in their response to different moisture regimes. The kabuli cultivars were found more sensitive compared with the desi types. Yield penalty exceeded 70% under severe drought. Conversely, cultivars tested under mild and severe stress drought showed average yield gain of 22 and 48%, respectively, relative to the irrigated treatment. Overall, over 50% yield gain can be obtained in drought‐affected rainfed production areas in Ethiopia using supplemental irrigation during pod setting to grain filling stages. For post‐rainy‐season crops relying on residual soil moisture, such as chickpea, breeding for shorter duration and resilient cultivars are reliable management approaches to minimize drought‐caused yield losses.
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- 2022
- Full Text
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22. Probiotic property of lactic acid bacteria from traditional fermented condiments: Datta and Awaze.
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Tigu, F., Assefa, F., Mehari, T., and Ashenafi, M.
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PROBIOTICS ,LACTIC acid bacteria ,CONDIMENTS ,FOOD fermentation ,ANTIBACTERIAL agents - Abstract
In this study, probiotic properties consisting of acid and bile tolerance, hydrophobicity, antibacterial activity, and antimicrobial susceptibility of lactic acid bacteria (LAB) isolated from two traditional fermented condiments (Datta and Awaze) were investigated. Of 100 isolates, 24 showed 54-97% survival rates at pH 2-3 and bile salt concentration of 0.3%-1%. Of the acid and bile tolerant isolates, only five showed marked hydrophobicity indicating tissue adherence potential . These five isolates inhibited test pathogenic bacteria to varying extents. These LAB isolates were susceptible to chloramphenicol, ampicillin, clindamycin, erythromycin, gentamycin, tetracycline and penicillin but resistant to vancomycin. All the five isolates were able to survive at levels of log 6 cfu/ml for >96 days in Datta stored at 4oC. These isolates belonged to LAB genera comprising Lactococcus (2) Leuconostoc (2) and Lactobacillus (1). [ABSTRACT FROM AUTHOR]
- Published
- 2016
23. Enhancing biological nitrogen removal from tannery effluent by using the efficient Brachymonas denitrificans in pilot plant operations
- Author
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Leta, Seyoum, Assefa, F., Dalhammar, Gunnel, Leta, Seyoum, Assefa, F., and Dalhammar, Gunnel
- Abstract
Laboratory scale and pilot plant reactors were inoculated with an efficient denitrifier, Brachymonas denitrificans (CCUG 45880), in order to evaluate whether a bio-augmentation approach can be used to enhance biological nitrogen removal from tannery effluents. To determine the effectiveness of the introduced strain, denitrifying activity in the activated sludge was monitored by nitrate uptake rate ( NUR) measurement of NO3-N. Fluorescent in situ hybridization ( FISH) technique was used to monitor the growth of the augmented species. The laboratory scale nitrate removal efficiency with the introduced B. denitrificans (3.7 +/- 0.6 mg NO3-N gVSS(-1) h(-1)) was higher than that of the activated sludge without the addition of the bacteria (3.5 +/- 0.7 mg NO3-N gVSS(-1) h(-1)); the NUR in the pilot plant after and before the introduction of the strain was also of the magnitude of 12.0 +/- 1.4 and 10.6 +/- 1.4 mg NO3- N gVSS(-1) day(-1), respectively. In situ hybridization results revealed that the introduced denitrifying bacteria significantly facilitated the development of a dense denitrifying bacterial population in the activated sludge, which enhanced in situ denitrification activity. FISH data indicated that once introduced, B. denitrificans remained abundant throughout the experimental period. The ability to seed a bioreactor with bacterial strain capable of removing target pollutants from tannery effluents in a mixed microbial community suggests that this approach could have commercial applications.
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- 2005
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24. Biological nitrogen and organic matter removal from tannery wastewater in pilot plant operations in Ethiopia
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Leta, Seyoum, Assefa, F., Gumaelius, Lena, Dalhammar, Gunnel, Leta, Seyoum, Assefa, F., Gumaelius, Lena, and Dalhammar, Gunnel
- Abstract
The objective of this study was to set-up a pilot plant and to evaluate its effectiveness for biological nitrogen and organic matter removal from tannery wastewater in Ethiopia. A pilot wastewater treatment plant consisting of a predenitrification-nitrification process was constructed and operated for 6 months. This was fed with a raw tannery wastewater obtained from the Modjo Tannery located 70 km south of the capital, Addis Ababa. Up to 98% total nitrogen and chemical oxygen demand, and 95% ammonium nitrogen removal efficiencies were achieved in the system. The average effluent ammonium nitrogen ranged from 8.4 mg l(-1) to 86.0 mg l(-1), whereas the average effluent for nitrate nitrogen ranged from 2.9 mg l(-1) to 4.4 mg l(-1). The average values of denitrification and nitrification rates determined by nitrate and ammonium uptake rates (NUR and AUR) were 8.0 mg NO3-N [g volatile suspended solids (VSS)](-1) h(-1) and 5.4 mg NH4-N (g VSS)(-1) h(-1), respectively, demonstrating that the treatment processes of the pilot plant were effective. Further studies of the effect of chromium III on AUR showed 50% inhibition at a concentration of 85 mg l(-1), indicating that this metal was not causing process inhibition during performance operations. Thus, the predenitrification-nitrification process was found to be efficient for simultaneous removal of nitrogen and organic substrates from tannery wastewaters.
- Published
- 2004
- Full Text
- View/download PDF
25. dentification of efficient denitrifying bacteria from tannery wastewaters in Ethiopia and a study of the effects of chromium III and sulphide on their denitrification rate
- Author
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Leta, Seyoum, Gumaelius, Lena, Assefa, F., Dalhammar, Gunnel, Leta, Seyoum, Gumaelius, Lena, Assefa, F., and Dalhammar, Gunnel
- Abstract
In order to identify potential microorganisms with high denitrifying capacity from tannery wastewaters, 1000 pure cultures of bacterial isolates from Modjo Tannery Pilot and Ethio-tannery wastewater treatment plants (WWTP), in Ethiopia, were investigated. Twenty-eight isolates were selected as efficient denitrifiers. These were Gram-negative rods, oxidase and catalase positive denitrifying organisms. The 28 denitrifying strains were further classified according to their biochemical fingerprints into three different phylogenetic groups (BPT1, BPT2 and BPT3) and seven singles. Isolates B79(T), B11, B12, B15, B28 and B38 belonging to the BPT3 cluster were found to be the most efficient denitrifying bacteria. All phenotypic studies, including cellular fatty acid profiles, showed that the 6 BPT3 isolates were closely related to each other. The 16S rRNA partial sequence analysis of type strain B79(T) (CCUG 45880) indicated a sequence similarity of 99% to Brachymonas denitrificans JCM9216 (D14320) in the beta-subdivision of proteobacteria. Further studies of the effects of chromium III and sulphide on the six Brachymonas denitrificans strains indicated that denitrification by the isolates were inhibited 50% at concentrations of 54 and 96 mg/l, respectively. The efficient isolates characterized in this study are of great value because of their excellent denitrifying properties and relatively high tolerance to the concentrations of toxic compounds (70 mg chromium/l and 160 mg sulphide/l) prevailing in tannery wastewaters., QC 20110826
- Published
- 2004
- Full Text
- View/download PDF
26. Assessment of Clients’ Satisfaction with Health Service Deliveries at Jimma University Specialized Hospital
- Author
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Assefa, F, primary, Mosse, A, additional, and H/Michael, Y, additional
- Published
- 2011
- Full Text
- View/download PDF
27. Characterization of tannery wastewater and assessment of downstream pollution profiles along Modjo River in Ethiopia
- Author
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Leta, Seyoum, Assefa, F., Dalhammar, Gunnel, Leta, Seyoum, Assefa, F., and Dalhammar, Gunnel
- Published
- 2003
28. Current and potential geographical distribution of the invasive plant Parthenium hysterophorus (Asteraceae) in eastern and southern Africa
- Author
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MCCONNACHIE, A J, primary, STRATHIE, L W, additional, MERSIE, W, additional, GEBREHIWOT, L, additional, ZEWDIE, K, additional, ABDUREHIM, A, additional, ABRHA, B, additional, ARAYA, T, additional, ASAREGEW, F, additional, ASSEFA, F, additional, GEBRE-TSADIK, R, additional, NIGATU, L, additional, TADESSE, B, additional, and TANA, T, additional
- Published
- 2010
- Full Text
- View/download PDF
29. Phenotypic characteristics of common bean (Phaseolus vulgaris)-nodulating rhizobia from some parts of southern Ethiopia
- Author
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Workalemahu, A, primary and Assefa, F, additional
- Published
- 2009
- Full Text
- View/download PDF
30. Environmental Impact of Coffee Processing Effluent on the Ecological Integrity of Rivers Found in Gomma Woreda of Jimma Zone, Ethiopia
- Author
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Kebede, Y. K., primary, Assefa, F., additional, and Amsalu, A., additional
- Published
- 2009
- Full Text
- View/download PDF
31. Characterization of Rhizobacteria Isolated from WildCoffea arabicaL.
- Author
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Muleta, D., primary, Assefa, F., additional, Hjort, K., additional, Roos, S., additional, and Granhall, U., additional
- Published
- 2009
- Full Text
- View/download PDF
32. Mycorrhizal Dependency and Response of Tomato (Lycopersicon esculentum) to Inoculation by Indigenous Arbuscular Mycorrhizal Fungi as Influenced by Available Soil Phosphorus Levels
- Author
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Chanie, T, primary and Assefa, F, additional
- Published
- 2008
- Full Text
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33. In vitro Antagonism of Rhizobacteria Isolated fromCoffea arabica L. against Emerging Fungal Coffee Pathogens
- Author
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Muleta, D., primary, Assefa, F., additional, and Granhall, U., additional
- Published
- 2007
- Full Text
- View/download PDF
34. Current and potential geographical distribution of the invasive plant Parthenium hysterophorus (Asteraceae) in eastern and southern Africa.
- Author
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MCCONNACHIE, A J, STRATHIE, L W, MERSIE, W, GEBREHIWOT, L, ZEWDIE, K, ABDUREHIM, A, ABRHA, B, ARAYA, T, ASAREGEW, F, ASSEFA, F, GEBRE-TSADIK, R, NIGATU, L, TADESSE, B, and TANA, T
- Subjects
PHYTOGEOGRAPHY ,PARTHENIUM hysterophorus ,INVASIVE plants ,CLIMATOLOGY - Published
- 2011
- Full Text
- View/download PDF
35. Characterization of Rhizobacteria Isolated from Wild Coffea arabica L.
- Author
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Muleta, D., Assefa, F., Hjort, K., Roos, S., and Granhall, U.
- Published
- 2009
- Full Text
- View/download PDF
36. In vitro Antagonism of Rhizobacteria Isolated from Coffea arabica L. against Emerging Fungal Coffee Pathogens.
- Author
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Muleta, D., Assefa, F., and Granhall, U.
- Published
- 2007
- Full Text
- View/download PDF
37. Biological nitrogen and organic matter removal from tannery wastewater in pilot plant operations in Ethiopia.
- Author
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Leta, S., Assefa, F., Gumaelius, L., and Dalhammar, G.
- Subjects
- *
PILOT plants , *TANNERIES , *ORGANIC compounds , *ORGANIC compounds removal (Sewage purification) , *DENITRIFICATION - Abstract
The objective of this study was to set-up a pilot plant and to evaluate its effectiveness for biological nitrogen and organic matter removal from tannery wastewater in Ethiopia. A pilot wastewater treatment plant consisting of a predenitrification-nitrification process was constructed and operated for 6 months. This was fed with a raw tannery wastewater obtained from the Modjo Tannery located 70 km south of the capital, Addis Ababa. Up to 98% total nitrogen and chemical oxygen demand, and 95% ammonium nitrogen removal efficiencies were achieved in the system. The average effluent ammonium nitrogen ranged from 8.4 mg l-1 to 86.0 mg l-1, whereas the average effluent for nitrate nitrogen ranged from 2.9 mg l-1 to 4.4 mg l-1. The average values of denitrification and nitrification rates determined by nitrate and ammonium uptake rates (NUR and AUR) were 8.0 mg NO3-N [g volatile suspended solids (VSS)]-1 h-1 and 5.4 mg NH4-N (g VSS)-1 h-1, respectively, demonstrating that the treatment processes of the pilot plant were effective. Further studies of the effect of chromium III on AUR showed 50% inhibition at a concentration of 85 mg l-1, indicating that this metal was not causing process inhibition during performance operations. Thus, the predenitrification-nitrification process was found to be efficient for simultaneous removal of nitrogen and organic substrates from tannery wastewaters. [ABSTRACT FROM AUTHOR]
- Published
- 2005
- Full Text
- View/download PDF
38. Intra-Unit Minorities in the Context of Ethno-National Federation in Ethiopia
- Author
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Assefa Fiseha
- Subjects
ethno-national federations ,rights of minorities ,territorial autonomy ,non-territorial autonomy ,Ethiopia ,Law in general. Comparative and uniform law. Jurisprudence ,K1-7720 - Abstract
After years of centralized rule emphasizing unity and territorial integrity, Ethiopia in 1991 adopted a federal system that aimed at accommodating its diversity. The system is designed to empower hitherto marginalized ethno-national groups by ensuring self-government in nine constituent units and redrawing boundaries to match with ethno-national boundaries. By designing constituent units, and, in some cases, local governments that ensure self-rule to major ethno-national groups, the constitution transforms these groups into majorities within the territories they control at constituent and local level. This article argues that while conferring territorial autonomy and self-rule to mobilized, territorially grouped ethno-national groups may be a step in the right direction to address the age-old ‘nationality question’, the design establishes a titular ethno-national group that claims exclusive control over territory, dominates public institutions, perpetuates majority rule and replicates the problems of the ‘nation-state’ at constituent-unit level. The combination of majority rule by titular ethno-national group and exclusive control over territory at constituent-unit level in a context of heterogeneous constituent units and increased inter-regional state mobility has therefore had grave consequences for intra-unit minorities. What the design provides is autonomy for a particular titular ethno-national group, not autonomy for all inhabitants in the constituent unit. Hence, the question arises: What institutional and policy options do we have to address the rights of intra-unit minorities in the states? It is argued that the process of empowering ethno-nationalist group at regional-state level was conducted without putting relevant institutional and policy mechanisms in place to minimize the marginalization of intra-unit minorities. The article therefore examines the institutional, political, legal and policy safeguards that exist for intra-unit minorities. It proposes four mechanisms that aim to address the concerns of intra-unit minorities: power-sharing as well as non-territorial autonomy; external checks by the federal government to monitor constituent units’ compliance with intra-unit minorities; and strict enforcement of human rights throughout the country. Enforcement of these packages of supplementary measures would mitigate the situation of intra-unit minorities and recast the conception of political power and territory in such a way that they are understood not as the exclusive property of a particular ethno-national group but a shared common good for all inhabitants of the constituent units.
- Published
- 2017
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39. Malnutrition, measles, mortality, and the humanitarian response during a famine in Ehiopia.
- Author
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Salama, Peter, Assefa, Fitsum, Talley, Liesel, Spiegel, Paul, Van der Veen, Albertien, Gotway, Carol A., Salama, P, Assefa, F, Talley, L, Spiegel, P, van Der Veen, A, and Gotway, C A
- Subjects
MORTALITY ,MALNUTRITION ,CAUSES of death ,STARVATION - Abstract
Context: The World Food Programme estimated that 10 million people were at risk of starvation in Ethiopia in 2000 but later reported that a famine had been averted. However, no population-based data on mortality or nutrition existed for Gode district, at the epicenter of the famine in the Somali region of Ethiopia.Objectives: To estimate mortality rates, determine the major causes of death, and estimate the prevalence of malnutrition among children and adults for the population of Gode district.Design and Setting: Two-stage cluster survey conducted from July 27 through August 1, 2000, which included anthropometric measures and 8-month retrospective mortality data collection.Participants: A total of 595 households comprising 4032 people living in Gode district of Ethiopia.Main Outcome Measures: Crude mortality rates and mortality rates for children younger than 5 years, causes of death, weight for height of less than -2 z scores among children aged 6 months to 5 years, and body mass index of less than 18.5 kg/m(2) among adults and older persons.Results: Of the 595 households, 346 (58.2%) were displaced from their usual places of residence. From December 1999 through July 2000, a total of 293 deaths occurred in the sample population; 159 (54.3%) deaths were among children younger than 5 years and 72 (24.6%) were among children aged 5 to 14 years. The crude mortality rate was 3.2/10 000 per day (95% confidence interval [CI], 2.4-3.8/10 000 per day), which is 3 times the cutoff used to define an emergency. The mortality rate for children younger than 5 years was 6.8/10 000 per day (95% CI, 5.4-8.2/10 000 per day). Approximately 77% of deaths occurred before major relief interventions began in April/May 2000. Wasting contributed to 72.3% of all deaths among children younger than 5 years. Measles alone or in combination with wasting accounted for 35 (22.0%) of 159 deaths among children younger than 5 years and for 12 (16.7%) of 72 deaths among children aged 5 to 14 years. The prevalence rate for wasting (weight for height of <-2 z score) among children aged 6 months to 5 years was 29.1% (95% CI, 24.7%-33.4%). Using a method to adjust body mass index for body shape, the prevalence of undernutrition (body mass index <18.5 kg/m(2)) among adults aged 18 to 59 years was 22.7% (95% CI, 17.9%-27.5%).Conclusions: To prevent unnecessary deaths, the humanitarian response to famine needs to be rapid, well coordinated, and based on sound epidemiological evidence. Public health interventions, such as mass measles vaccination campaigns with coverage extended to children aged 12 to 15 years should be implemented as the first priority. The prevalence of wasting and undernutrition among children and adults, respectively, should be assessed in all prolonged, severe famines. [ABSTRACT FROM AUTHOR]- Published
- 2001
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- View/download PDF
40. In vitro Antagonism of Rhizobacteria Isolated from Coffea arabicaL. against Emerging Fungal Coffee Pathogens
- Author
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Muleta, D., Assefa, F., and Granhall, U.
- Abstract
In vitro antagonistic effects of rhizobacteria associated with Coffea arabica L. against some fungal coffee pathogens were studied. The aims were to screen indigenous coffee‐associated isolates for their inherent antagonistic potential against major coffee wilt diseases induced by Fusariumspp. Antagonistic effects, siderophore, HCN and lytic enzyme production were determined on standard solid media. Chemical methods were employed to categorize the major types of siderophores. From a total of 212 rhizobacterial isolates tested, over 10 % (all Pseudomonasand Bacillusspp.) exhibited remarkable inhibition against Fusariumspp. One isolate AUPB24 (P. chlororaphis) showed maximum inhibition of mycelial growth against all fungal pathogens tested, whereas other isolates were mostly inhibitory to F. stilboidesand F. oxysporum. The isolate AUBB20 (B. subtilis) was most antagonistic to F. xylarioides. Of the rhizobacterial isolates tested, 67 % produced siderophores and 35 % produced HCN. Many strains (all Pseudomonasspp.) produced siderophores of the hydroxamate type and only a small proportion produced those of the catecholate type. Few antagonists showed chitinase activity. The production of siderophores and HCN by Pseudomonasspp., lipase and protease by all antagonists and β‐1,3‐glucanase by several Bacillusspp. could be considered the major mechanisms involved in the inhibition of fungal growth. The in vitro results provide the first evidence of an antagonistic effect of coffee‐associated rhizobacteria against the emerging fungal coffee pathogens F. stilboidesand F. xylarioidesand indicate the potential of both bacterial groups for biological control of coffee wilt diseases.
- Published
- 2007
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41. Exploring stress-tolerant plant growth-promoting rhizobacteria from groundnut rhizosphere soil in semi-arid regions of Ethiopia.
- Author
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Beshah A, Muleta D, Legese G, and Assefa F
- Subjects
- Ethiopia, Stress, Physiological, Indoleacetic Acids metabolism, Droughts, Soil chemistry, Arachis microbiology, Arachis growth & development, Arachis metabolism, Bacteria metabolism, Rhizosphere, Soil Microbiology
- Abstract
The potential of rhizobacteria with plant growth promoting (PGP) traits in alleviating abiotic stresses, especially drought, is significant. However, their exploitation in the semi-arid regions of Ethiopian soils remains largely unexplored. This research aimed to isolate and evaluate the PGP potential of bacterial isolates collected from groundnut cultivation areas in Ethiopia. Multiple traits were assessed, including phosphate solubilization, indole-3-acetic acid (IAA) production, ammonia production, salt and heavy metal tolerance, drought tolerance, enzyme activities, hydrogen cyanide production, antibiotic resistance, and antagonistic activity against fungal pathogens. The identification of potent isolates was carried out using MALDI-TOF MS. Out of the 82 isolates, 63 were gram-negative and 19 were gram-positive. Among them, 19 isolates exhibited phosphate solubilization, with AAURB 34 demonstrating the highest efficiency, followed by AURB 12. Fifty-six isolates produce IAA in varying amounts and all isolates produce ammonia with AAURB12, AAURB19, and AAURB34 displaying strong production. Most isolates demonstrated tolerance to temperatures up to 40°C and salt concentrations up to 3%. Notably, AAURB12 and AAURB34 exhibited remarkable drought tolerance at an osmotic potential of -2.70 Mpa. When subjected to levels above 40%, the tested isolates moderately produced lytic enzymes and hydrogen cyanide. The isolates displayed resistance to antibiotics, except gentamicin, and all isolates demonstrated resistance to zinc, with 81-91% showing resistance to other heavy metals. AAURB34 and AAURB12 exhibited suppression against fungal pathogens, with percent inhibition of 38% and 46%, respectively. Using MALDI-TOF MS, the promising PGP isolates were identified as Bacillus megaterium, Bacillus pumilus, and Enterobacter asburiae. This study provides valuable insights into the potential of rhizobacteria as PGP agents for mitigating abiotic stresses and contribute to the understanding of sustainable agricultural practices in Ethiopia and similar regions facing comparable challenges.
- Published
- 2024
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42. Impact of brewery sludge application on heavy metal build-up, translocation, growth and yield of bread wheat ( Triticum aestivum L.) crop in Northern Ethiopia.
- Author
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Jebesa WT, Astatkie T, Zerfu A, Kenea HD, Abamecha N, Shumuye M, Abera G, Kidane A, Hirko M, and Assefa F
- Abstract
In a field study, the impact of different levels of brewery sludge (BS) enrichment on Triticum aestivum L. (wheat plants) was examined in terms of growth, yield, heavy metal absorption, and potential health risks linked to plant consumption. Using a randomized complete block design with seven treatments and three blocks, the study showed that applying up to 12 t ha
-1 brewery sludge significantly improved all agronomic parameters (except harvest index) compared to control and mineral-fertilized soil. Heavy metal translocation was generally low, except for Cu and Pb. The sequence of heavy metal translocation was Cu > Pb > Cd > Ni > Zn > Mn > Cr from soil to spikes and Cu > Zn > Mn > Pb > Ni > Cd > Cr from soil to grain. Heavy metal loads were mostly higher in roots than in the above-ground crop parts. The target hazard quotient (THQ), hazard index (HI), and target cancer risk (TCR) within wheat grain remained within safe limits for all BS treatments. Consequently, consuming this wheat grain is considered safe regarding heavy metals. Thus, utilizing brewery sludge at 12 t ha-1 as a fertilizer for wheat production and as an alternative method for sludge disposal is plausible., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2024 The Authors. Published by Elsevier Ltd.)- Published
- 2024
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43. The effects of seedling transplanting on growth and yield performance of maize ( Zea mays L.) for climate change resilience in Burie District, Northwestern Ethiopia: Dataset Article.
- Author
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Yigermal H, Nakachew K, and Assefa F
- Abstract
Maize is produced throughout the world and it is also a primary staple food crop in many developing countries. The field experiment was conducted during the main rainy season of 2018 in Burie district to study the effects of types and growth stages of seedlings on the growth and yield of transplanted maize (Zea mays L.). Factorial combinations of two types of seedlings (bare rooted and poly bagged); five levels of seedling's growth stages (seedlings of 1, 2, 3, 4, and 5 true leaf/ves) and one control (direct seeded) were laid down in randomized complete block design with three replications. Data on phenological, vegetative growth and yield-related parameters were collected following standard methods and procedures. All data were subjected to analysis of variance using SAS software, and mean separation for significant treatments was done by LSD. Both main effects affected the number of grains cob
-1 , grain, and stover yield highly significantly and days to 50% silking very highly significantly. Types of seedlings affected days to 50% tasselling significantly; and days to 90% physiological maturity and plant height highly significantly. In addition, types of seedlings affected the number of cobs plant-1 , cob length, number of grains row-1 and biomass yield, very highly significantly. Seedlings' growth stages had a very highly significant effect on days to 50% tasseling and days to 90% physiological maturity. The number of cobs plant-1 , cob length, number of grains row-1 and biomass yield were also highly significantly affected by seedlings' growth stages. The interaction effect was highly significant on the number of cobs plant-1 , grain, and stover yield and very highly significant on the harvest index. The highest (10.7t ha-1 ) grain yield of maize was found from the transplantation of polybagged seedlings at four true leaf stages. Although it is difficult to conclude based on one season and one location research trial, transplanting of poly-bagged seedlings at four true leaf stages gave superior grain yield. This treatment combination also gave the highest net benefit with an acceptable range of marginal rate of return. Therefore, transplanting polybagged seedlings at four true leaf stages is economically feasible and can be recommended tentatively for Burie District. However; it's also advised to repeat the study in areas having terminal moisture stress for maize production., (© 2024 The Author(s).)- Published
- 2024
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44. The effect of seed and nitrogen-phosphorous fertilizer rates on growth and yield components of bread wheat ( Triticum aestivum L.) in Burie District, Northwestern Ethiopia: Dataset article.
- Author
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Nakachew K, Assefa F, and Yigermal H
- Abstract
In the absence of a recommended optimal seed rate and nitrogen-phosphorous (NP) fertilizers application for a specific area, achieving maximum bread wheat productivity becomes challenging. To address this issue, this field experiment was conducted to evaluate the effect of seed and NP fertilizer rates on growth, yield, and yield components of bread wheat ( Triticum aestivum L.). Factorial combinations of four levels of seed rates of bread wheat (100, 120, 150, and 200 kg ha
-1 ) and four levels of NP fertilizers rates (64-46, 87-46, 96-69, and 119-69 kg ha-1 of N and P2 O5 ) were laid down in randomized complete block design with three replications. The remaining necessary agronomic practices and crop management activities were undertaken uniformly. The data presented in this dataset article includes phenological, growth, yield, and yield component parameters that were collected timely following their standard methods and procedures. All the collected data were subjected to analysis of variance (ANOVA) which was carried out using the SAS version 9.0 software computer program's General Linear Model (GLM) procedure [1]. the residuals were evaluated, as described in Montgomery [2], to confirm the normal distribution and homogeneous variance model assumptions on the error terms for each response variable. The independence assumption is upheld due to the randomization of the sixteen (16) treatment combinations within each block. In cases where a treatment effect was found to be significant, a multiple means comparison was conducted at a 5% significance level using Fisher's LSD method to create letter groupings. Additionally, correlation analysis was carried out using the Pearson correlation procedure in SAS. This dataset article provides insights into how seed rate and NP fertilizer rates impact bread wheat productivity, as well as the economic viability of optimal seed rate and NP fertilizer rates on bread wheat productivity. The information presented can serve as a valuable resource for researchers looking to examine the supplementary data and methods in detail, potentially leading to new research avenues. Furthermore, it has the potential to foster collaborations and enhance the credibility of the current research data within the scientific community, making it accessible for wider use., (© 2024 The Author(s).)- Published
- 2024
- Full Text
- View/download PDF
45. Data on the effects of brewery sludge and blended NPS fertilizer rates on yield and yield components of maize ( Zea Mays L.) in North Mecha District, Northwestern Ethiopia.
- Author
-
Assefa F, Gebremedhin Z, and Alem T
- Abstract
Maize is one of the most important cereal crops in Ethiopia. However, its yield is lower than its potential mainly due to low soil fertility problems. Therefore, this field experiment was conducted during 2021 main rainy season with the objective of improving maize production through application of brewery sludge and blended NPS fertilizers in North Mecha district, Northwestern Ethiopia. Factorial combination of three levels of brewery sludge (0, 10 and 20 t ha
-1 ) and four levels of blended NPS fertilizer rates (0, 50, 100 and 150 kg ha-1 ) were laid out in RCBD with three replications. The remaining necessary agronomic practices and crop management activities were undertaken uniformly. The data presented under this dataset article includes phenological Parameters (i.e. Days to 50 % emergence, Days to 50 % tasseling, Days to 50 % silking and Days to 90 % physiological maturity), growth parameters (i.e. Plant height, leaf area and leaf area index) and yield and yield component parameters (i.e. Number of ears plant-1 , Above ground dry biomass yield, Ear length, Number of rows ear-1 , Number of kernels row-1 , Number of kernels ear-1 , Grain yield, Thousand kernels weight, Stover yield and Harvest index). All the collected data were subjected to analysis of variance (ANOVA) and the analysis was carried out using the SAS version 9.0 software computer program's General Linear Model (GLM) procedure [1]. As described in Montgomery [2], the residuals were examined to verify the normal distribution and homogeneous variance model assumptions on the error terms for each response variable. Because the twelve treatment combinations were randomized within each block, the independence assumption is valid. When a treatment effect was significant, multiple means comparison was performed at a 5 % level of significance using the least significant difference (Fisher's LSD) method to generate letter groupings and correlation analysis was performed using the Pearson correlation procedure found in SAS. This dataset article, therefore gives information about the effects of brewery sludge and blended NPS fertilizer rates on maize productivity. Additionally, it provides the economic feasibility of brewery sludge integrated application with blended NPS fertilizer than sole application of blended chemical fertilizers. Hence, this information can allow other researchers to review the supplement data, methods, and make detailed analysis, which possibly giving rise to new lines of inquiry. This can also give rise to new collaborations and boost the reputation of the present research data within the scientific community and to make it available to everyone around the subject matter to use as they wish., (© 2023 The Author(s).)- Published
- 2023
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46. The role of sensory and sympathetic nerves in craniofacial bone regeneration.
- Author
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Assefa F
- Subjects
- Vasoactive Intestinal Peptide metabolism, Neurons metabolism, Bone and Bones metabolism, Substance P metabolism, Calcitonin Gene-Related Peptide metabolism, Neuropeptide Y metabolism
- Abstract
Multiple factors regulate the regeneration of craniofacial bone defects. The nervous system is recognized as one of the critical regulators of bone mass, thereby suggesting a role for neuronal pathways in bone regeneration. However, in the context of craniofacial bone regeneration, little is known about the interplay between the nervous system and craniofacial bone. Sensory and sympathetic nerves interact with the bone through their neuropeptides, neurotransmitters, proteins, peptides, and amino acid derivates. The neuron-derived factors, such as semaphorin 3A (SEMA3A), substance P (SP), calcitonin gene-related peptide (CGRP), neuropeptide Y (NPY), and vasoactive intestinal peptide (VIP), possess a remarkable role in craniofacial regeneration. This review summarizes the roles of these factors and recently published factors such as secretoneurin (SN) and spexin (SPX) in the osteoblast and osteoclast differentiation, bone metabolism, growth, remodeling and discusses the novel application of nerve-based craniofacial bone regeneration. Moreover, the review will facilitate understanding the mechanism of action and provide potential treatment direction for the craniofacial bone defect., Competing Interests: Declaration of Competing Interest The author has no conflict of financial conflict of interest., (Copyright © 2023 Elsevier Ltd. All rights reserved.)
- Published
- 2023
- Full Text
- View/download PDF
47. Kerosene Biodegradation by Highly Efficient Indigenous Bacteria Isolated From Hydrocarbon-Contaminated Sites.
- Author
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Bekele GK, Gebrie SA, Abda EM, Sinshaw G, Haregu S, Negie ZW, Tafesse M, and Assefa F
- Abstract
Kerosene is widely used in Ethiopia as a household fuel (for lighting and heating), as a solvent in paint and grease, and as a lubricant in glass cutting. It causes environmental pollution and escorts to loss of ecological functioning and health problems. Therefore, this research was designed to isolate, identify, and characterize indigenous kerosene-degrading bacteria that are effective in cleaning ecological units that have been contaminated by kerosene. Soil samples were collected from hydrocarbon-contaminated sites (flower farms, garages, and old-aged asphalt roads) and spread-plated on mineral salt medium (Bushnell Hass Mineral Salts Agar Medium: BHMS), which consists of kerosene as the only carbon source. Seven kerosene-degrading bacterial species were isolated, 2 from flower farms, 3 from garage areas, and 2 from asphalt areas. Three genera from hydrocarbon-contaminated sites were identified, including Pseudomonas , Bacillus , and Acinetobacter using biochemical characterization and the Biolog database. Growth studies in the presence of various concentrations of kerosene (1% and 3% v/v) showed that the bacterial isolates could metabolize kerosene as energy and biomass. Thereby, a gravimetric study was performed on bacterial strains that proliferated well on a BHMS medium with kerosene. Remarkably, bacterial isolates were able to degrade 5% kerosene from 57.2% to 91% in 15 days. Moreover, 2 of the most potent isolates, AUG2 and AUG1, resulted in 85% and 91% kerosene degradation, respectively, when allowed to grow on a medium containing kerosene. In addition, 16S rRNA gene analysis indicated that strain AAUG1 belonged to Bacillus tequilensis , whereas isolate AAUG showed the highest similarity to Bacillus subtilis . Therefore, these indigenous bacterial isolates have the potential to be applied for kerosene removal from hydrocarbon-contaminated sites and the development of remediation approaches., Competing Interests: The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article., (© The Author(s) 2023.)
- Published
- 2023
- Full Text
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48. Evaluation of inorganic phosphate solubilizing efficiency and multiple plant growth promoting properties of endophytic bacteria isolated from root nodules Erythrina brucei.
- Author
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Berza B, Sekar J, Vaiyapuri P, Pagano MC, and Assefa F
- Subjects
- Endophytes, Phosphates metabolism, Bacteria, Triticum microbiology, Soil, Iron metabolism, Erythrina metabolism
- Abstract
Background: In soils, phosphorous (P) mostly exists in fixed/insoluble form and unavailable for plants use in soil solution, hence it is in scarcity. P is fixed in the form of aluminium, iron and manganese phosphates in acidic soils and calcium phosphate in alkaline soils. Phosphate solubilizing bacteria, the ecological engineers play a pivotal role in the mobilization of fixed forms of P by using different mechanisms. The objectives of this study were to evaluate inorganic phosphate solubilizing efficiency and other multiple plant growth promoting traits of Erythrina brucei root nodule endophytic bacteria and to investigate effects of the selected endophytic bacteria on the growth of wheat plant under phosphorous deficient sand culture at greenhouse conditions., Results: Among a total of 304 passenger endophytic bacteria, 119 (39%) exhibited tricalcium phosphate (TCP) solubilization; however, none of them were formed clear halos on solid medium supplemented with aluminum phosphate (Al-P) or iron phosphate (Fe-P). Among 119 isolates, 40% exhibited IAA production. The selected nine potential isolates also exhibited potentials of IAA, HCN, NH
3 and/or hydrolytic enzymes production. All the selected isolates were potential solubilizers of the three inorganic phosphates (Al-P, Fe-P and TCP) included in liquid medium. The highest values of solubilized TCP were recorded by isolates AU4 and RG6 (A. soli), 108.96 mg L-1 and 107.48 mg L-1 , respectively at sampling day3 and 120.36 mg L-1 and 112.82 mg L-1 , respectively at day 6. The highest values of solubilized Al-P and Fe-P were recorded by isolate RG6, 102.14 mg L-1 and 96.07 mg L-1 , respectively at sampling days 3 and 6, respectively. The highest IAA, 313.61 µg mL-1 was recorded by isolate DM17 (Bacillus thuringiensis). Inoculation of wheat with AU4, RG6 and RG5 (Acinetobacter soli) increased shoot length by 11, 17.4 and 14.6%, respectively compared to the negative control. Similarly, 76.9, 69.2 and 53.8% increment in shoot dry weight is recorded by inoculation with RG6, AU4 and RG5, respectively. These nine potential endophytic isolates are identified to Gluconobacter cerinus (4), Acinetobacter soli (3), Achromobacter xylosoxidans (1) and Bacillus thuringiensis (1)., Conclusion: AU4, RG6 and RG5 can be potential bio-inoculants candidates as low cost agricultural inputs in acidic and/or alkaline soils for sustainable crop production., (© 2022. The Author(s).)- Published
- 2022
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49. Plant growth-promoting rhizobacterial inoculation to improve growth, yield, and grain nutrient uptake of teff varieties.
- Author
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Tsegaye Z, Alemu T, Desta FA, and Assefa F
- Abstract
Inoculation of plant growth-promoting rhizobacteria (PGPR) improves the growth, yield, and plant nutrient uptake, as well as rhizosphere fertility, without harming the environment and human health. This study aimed to examine the effect of either individual or consortium of PGP bacterial inoculation on the growth, yield, and grain nutrient uptake of teff varieties. Three potential PGPR strains (i.e., Pseudomonas fluorescens biotype G , Enterobacter cloacae ss disolvens , and Serratia marcescens ss marcescens ) were used for this study. Field evaluation was carried out in RCBD with 5 treatments. Highly significant ( P < 0.001) differences were observed among treatments for plant height (PH), panicle length (PL), number of the total spike (NTS), shoot dry weight (SDW), grain yield (GY), and straw yield (SY). There was also teff variety that significantly ( P < 0.01) affects PL, SDW, and SY. However, the interaction effect of the two factors (treatment*variety) did not significantly influence teff agronomic traits and grain nutrient uptake. The highest PH (133.5 cm), PL (53.2), NTS (30.9), SDW (18.1 t/ha), SY (10.7 t/ha), and GY (2.7 t/ha) were observed on Dukem variety (Dz-01-974) inoculated with PGPR consortium. Wherein 2.2 fold increase was observed in grain yield per hectare over the control. Inoculation of PGPR consortium showed better performance in promoting plant growth, yield, and grain nutrient uptake of teff varieties compared with the individual PGP bacterial application, and PGPR consortium could be used as inoculants to enhance teff production and productivity., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Tsegaye, Alemu, Desta and Assefa.)
- Published
- 2022
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50. Management Outcome and Factors Associated with Pediatric Surgical Patient Admitted to Arbaminch General Hospital, Southern Ethiopia, 2021: Retrospective Cross-Sectional Study.
- Author
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Moga F, Assefa F, Wondwossen K, and Berhan Y
- Abstract
Background: Pediatric surgical situations are often disregarded around the world, particularly in developing countries. The number of children hospitalized for surgical reasons has climbed dramatically. There is extensive research on the management outcome of pediatric surgical admissions in industrialized countries, but developing countries have paid little attention to it. Furthermore, to the best of the authors' knowledge, there has been no research in the study area on the management outcome of pediatric surgical patient admission., Objective: To assess management outcome and factors associated with pediatric surgical patients admitted to Arbaminch General Hospital, Southern Ethiopia, 2021., Method: An institution-based retrospective cross-sectional study design was employed among 265 children with surgical problems. Data were collected from patients' medical records using pretested data collection checklist. Epi Data 4.2 was used to enter data, and data were exported to SPSS version 25 for analysis. Those variables with P -value ≤0.25 in bivariable analysis were entered into multivariable logistic regression analysis, and statistical significance was declared at P < 0.05., Result: A total of 265 surgically admitted children were included in this study. About 26% of study subjects were discharged with unfavorable management outcome. Children admitted due to trauma cause (AOR: 5.753, 95% CI: 2.366-13.987), children with a preexisting medical condition (AOR: 3.240, 95% CI: 1.436-7.310), children with an early complication (AOR: 2.515, 95% CI: 1.130-5.599), presenting to hospital ≥24 hr after the onset (AOR:8.351, 95% CI: 2.089-33.381), hospital stay >7 days (AOR: 10.671, 95% CI: 1.363-83.546), and children treated with surgery (AOR: 2.742, 95% CI: 1.137-6.611) were associated with unfavorable management outcome. Conclusion and recommendations : Twenty-six percent of patients were discharged with unfavorable outcome. Reasons for admission, preexisting medical condition, early complications, duration of hospital presentation, length of hospitalization, and type of management were all linked to the outcome of pediatric surgical admission. To have a good outcome, early identification and treatment of the cause are required, as well as well-equipped surgical care centers., Competing Interests: The authors declare that they have no conflicts of interest., (Copyright © 2022 Fikre Moga et al.)
- Published
- 2022
- Full Text
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