1. Photodynamic activity and photoantimicrobial chemotherapy studies of ferrocene-substituted 2-thiobarbituric acid.
- Author
-
Babu B, Ochappan T, Asraf Ali T, Mack J, Nyokong T, and Gopalakrishnan Sethuraman M
- Subjects
- Anti-Bacterial Agents chemistry, Anti-Bacterial Agents radiation effects, Escherichia coli drug effects, Ferrous Compounds chemistry, Ferrous Compounds radiation effects, History, Medieval, Humans, Hydroxyl Radical metabolism, Light, MCF-7 Cells, Metallocenes chemistry, Metallocenes radiation effects, Microbial Sensitivity Tests, Oxidation-Reduction radiation effects, Photosensitizing Agents chemistry, Photosensitizing Agents radiation effects, Staphylococcus aureus drug effects, Thiobarbiturates chemistry, Thiobarbiturates radiation effects, Anti-Bacterial Agents pharmacology, Ferrous Compounds pharmacology, Metallocenes pharmacology, Photosensitizing Agents pharmacology, Thiobarbiturates pharmacology
- Abstract
A ferrocene-substituted thiobarbituric acid (FT) has been synthesized to explore its photophysical properties and photodynamic and photoantimicrobial chemotherapy activities. FT has an intense metal-to-ligand charge transfer (MLCT) band at ca. 575 nm. The ferrocene moiety of FT undergoes photooxidation to form a ferrocenium species which in turn produces hydroxyl radical in an aqueous environment, which was confirmed via the bleaching reaction of p-nitrosodimethylaniline (RNO). FT exhibits efficient PDT activity against MCF-7 cancer cells with an IC
50 value of 5.6 μM upon irradiation with 595 nm for 30 min with a Thorlabs M595L3 LED (240 mW cm-2 ). Photodynamic inactivation of Staphylococcus aureus and Escherichia coli by FT shows significant activity with log reduction values of 6.62 and 6.16 respectively, under illumination for 60 min at 595 nm. These results demonstrate that ferrocene-substituted thiobarbituric acids merit further study for developing novel bioorganometallic PDT agents., (Copyright © 2021 Elsevier Ltd. All rights reserved.)- Published
- 2021
- Full Text
- View/download PDF