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1. Automated identification of leukocyte subsets improves standardization of database-guided expert-supervised diagnostic orientation in acute leukemia: a EuroFlow study

Author

Lhermitte, Ludovic, Barreau, Sylvain, Morf, Daniela, Fernandez, Paula, Grigore, Georgiana, Barrena, Susana, de Bie, Maaike, Flores-Montero, Juan, Brüggemann, Monika, Mejstrikova, Ester, Nierkens, Stefan, Burgos, Leire, Caetano, Joana, Gaipa, Giuseppe, Buracchi, Chiara, da Costa, Elaine Sobral, Sedek, Lukasz, Szczepański, Tomasz, Aanei, Carmen-Mariana, van der Sluijs-Gelling, Alita, Delgado, Alejandro Hernández, Fluxa, Rafael, Lecrevisse, Quentin, Pedreira, Carlos E., van Dongen, Jacques J.M., Orfao, Alberto, van der Velden, Vincent H.J., van Dongen, J. J.M., Bitter, W.M., Lubbers, B.R., Teodosio, C.I., Zlei, M., van der Sluijs-Gelling, A.J., de Bie, F., de Bruin-Versteeg, S., van der Burg, M., Schilham, M.W., van der Velden, V. H.J., Langerak, A.W., te Marvelde, J., Bras, A.E., Schilperoord-Vermeulen, J., Jugooa, R., Heezen, K.C., Orfao, A., Almeida, J., Vidriales, M.B., Flores-Montero, J., Pérez-Andrés, M., Matarraz, S., Martín, L., Lecrevisse, Q., Pérez-Morán, J.J., Puig, N., Almeida, A. Medina, Gomes da Silva, M., Faria, T., Brüggemann, M., Ritgen, M., Szczepanowski, M., Kohlscheen, S., Laqua, A., Harbst, E., Finke, J., Asnafi, V., Lhermitte, L., Duroyon, E., Trka, J., Hrusak, O., Kalina, T., Mejstrikova, E., Novakova, M., Thurner, D., Kanderova, V., Szczepanski, T., Sędek, L., Bulsa, J., Slota, L., Kulis, J., Pedreira, C.E., da Costa, E. Sobral, Nierkens, S., de Jong, A., de Koning, A., Lima, M., Santos, A.H., Böttcher, S., Lange, S., Engelmann, R., Paape, D., Machka, C., Gaipa, G., Burracchi, C., Bugarin, C., Lopez-Granados, E., del Pino Molina, L., Campos-Guyotat, L., Aanei, C., Miguel, J. F. San, Paiva, B., Burgos, L., Villamor-Casas, N., Magnano, L., Philippé, J., Bonroy, C., Denys, B., Willems, A., Breughe, P., de Wolf, J., Sousa, A.E., Silva, S.L., Fernandez, P., and Morf, D.

Published
2021
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2. Prognostic value of PET / CT and CT in T‐cell lymphoblastic lymphoma/leukaemia patients: A retrospective cohort study of 145 patients

Author

Rouzaud, C., primary, Vercellino, L., additional, De Kerviler, E., additional, Raffoux, E., additional, Balsat, M., additional, Marcais, A., additional, Dourthe, M.‐E., additional, Meignin, V., additional, Asnafi, V., additional, MacIntyre, E., additional, Boissel, N., additional, and Lengliné, E., additional

Published
2023
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6. Automated identification of leukocyte subsets improves standardization of database-guided expert-supervised diagnostic orientation in acute leukemia: a EuroFlow study

Author

Lhermitte, L, Barreau, S, Morf, D, Fernandez, P, Grigore, G, Barrena, S, de Bie, M, Flores-Montero, J, Bruggemann, M, Mejstrikova, E, Nierkens, S, Burgos, L, Caetano, J, Gaipa, G, Buracchi, C, da Costa, E, Sedek, L, Szczepanski, T, Aanei, C, van der Sluijs-Gelling, A, Delgado, A, Fluxa, R, Lecrevisse, Q, Pedreira, C, van Dongen, J, Orfao, A, van der Velden, V, Bitter, W, Lubbers, B, Teodosio, C, Zlei, M, de Bie, F, de Bruin-Versteeg, S, van der Burg, M, Schilham, M, Langerak, A, te Marvelde, J, Bras, A, Schilperoord-Vermeulen, J, Jugooa, R, Heezen, K, Almeida, J, Vidriales, M, Perez-Andres, M, Matarraz, S, Martin, L, Perez-Moran, J, Puig, N, Almeida, A, Gomes da Silva, M, Faria, T, Ritgen, M, Szczepanowski, M, Kohlscheen, S, Laqua, A, Harbst, E, Finke, J, Asnafi, V, Duroyon, E, Trka, J, Hrusak, O, Kalina, T, Novakova, M, Thurner, D, Kanderova, V, Bulsa, J, Slota, L, Kulis, J, de Jong, A, de Koning, A, Lima, M, Santos, A, Bottcher, S, Lange, S, Engelmann, R, Paape, D, Machka, C, Burracchi, C, Bugarin, C, Lopez-Granados, E, del Pino Molina, L, Campos-Guyotat, L, Miguel, J, Paiva, B, Villamor-Casas, N, Magnano, L, Philippe, J, Bonroy, C, Denys, B, Willems, A, Breughe, P, de Wolf, J, Sousa, A, Silva, S, Lhermitte L., Barreau S., Morf D., Fernandez P., Grigore G., Barrena S., de Bie M., Flores-Montero J., Bruggemann M., Mejstrikova E., Nierkens S., Burgos L., Caetano J., Gaipa G., Buracchi C., da Costa E. S., Sedek L., Szczepanski T., Aanei C. -M., van der Sluijs-Gelling A., Delgado A. H., Fluxa R., Lecrevisse Q., Pedreira C. E., van Dongen J. J. M., Orfao A., van der Velden V. H. J., Bitter W. M., Lubbers B. R., Teodosio C. I., Zlei M., van der Sluijs-Gelling A. J., de Bie F., de Bruin-Versteeg S., van der Burg M., Schilham M. W., Langerak A. W., te Marvelde J., Bras A. E., Schilperoord-Vermeulen J., Jugooa R., Heezen K. C., Almeida J., Vidriales M. B., Perez-Andres M., Matarraz S., Martin L., Perez-Moran J. J., Puig N., Almeida A. M., Gomes da Silva M., Faria T., Ritgen M., Szczepanowski M., Kohlscheen S., Laqua A., Harbst E., Finke J., Asnafi V., Duroyon E., Trka J., Hrusak O., Kalina T., Novakova M., Thurner D., Kanderova V., Bulsa J., Slota L., Kulis J., de Jong A., de Koning A., Lima M., Santos A. H., Bottcher S., Lange S., Engelmann R., Paape D., Machka C., Burracchi C., Bugarin C., Lopez-Granados E., del Pino Molina L., Campos-Guyotat L., Aanei C., Miguel J. F. S., Paiva B., Villamor-Casas N., Magnano L., Philippe J., Bonroy C., Denys B., Willems A., Breughe P., de Wolf J., Sousa A. E., Silva S. L., Lhermitte, L, Barreau, S, Morf, D, Fernandez, P, Grigore, G, Barrena, S, de Bie, M, Flores-Montero, J, Bruggemann, M, Mejstrikova, E, Nierkens, S, Burgos, L, Caetano, J, Gaipa, G, Buracchi, C, da Costa, E, Sedek, L, Szczepanski, T, Aanei, C, van der Sluijs-Gelling, A, Delgado, A, Fluxa, R, Lecrevisse, Q, Pedreira, C, van Dongen, J, Orfao, A, van der Velden, V, Bitter, W, Lubbers, B, Teodosio, C, Zlei, M, de Bie, F, de Bruin-Versteeg, S, van der Burg, M, Schilham, M, Langerak, A, te Marvelde, J, Bras, A, Schilperoord-Vermeulen, J, Jugooa, R, Heezen, K, Almeida, J, Vidriales, M, Perez-Andres, M, Matarraz, S, Martin, L, Perez-Moran, J, Puig, N, Almeida, A, Gomes da Silva, M, Faria, T, Ritgen, M, Szczepanowski, M, Kohlscheen, S, Laqua, A, Harbst, E, Finke, J, Asnafi, V, Duroyon, E, Trka, J, Hrusak, O, Kalina, T, Novakova, M, Thurner, D, Kanderova, V, Bulsa, J, Slota, L, Kulis, J, de Jong, A, de Koning, A, Lima, M, Santos, A, Bottcher, S, Lange, S, Engelmann, R, Paape, D, Machka, C, Burracchi, C, Bugarin, C, Lopez-Granados, E, del Pino Molina, L, Campos-Guyotat, L, Miguel, J, Paiva, B, Villamor-Casas, N, Magnano, L, Philippe, J, Bonroy, C, Denys, B, Willems, A, Breughe, P, de Wolf, J, Sousa, A, Silva, S, Lhermitte L., Barreau S., Morf D., Fernandez P., Grigore G., Barrena S., de Bie M., Flores-Montero J., Bruggemann M., Mejstrikova E., Nierkens S., Burgos L., Caetano J., Gaipa G., Buracchi C., da Costa E. S., Sedek L., Szczepanski T., Aanei C. -M., van der Sluijs-Gelling A., Delgado A. H., Fluxa R., Lecrevisse Q., Pedreira C. E., van Dongen J. J. M., Orfao A., van der Velden V. H. J., Bitter W. M., Lubbers B. R., Teodosio C. I., Zlei M., van der Sluijs-Gelling A. J., de Bie F., de Bruin-Versteeg S., van der Burg M., Schilham M. W., Langerak A. W., te Marvelde J., Bras A. E., Schilperoord-Vermeulen J., Jugooa R., Heezen K. C., Almeida J., Vidriales M. B., Perez-Andres M., Matarraz S., Martin L., Perez-Moran J. J., Puig N., Almeida A. M., Gomes da Silva M., Faria T., Ritgen M., Szczepanowski M., Kohlscheen S., Laqua A., Harbst E., Finke J., Asnafi V., Duroyon E., Trka J., Hrusak O., Kalina T., Novakova M., Thurner D., Kanderova V., Bulsa J., Slota L., Kulis J., de Jong A., de Koning A., Lima M., Santos A. H., Bottcher S., Lange S., Engelmann R., Paape D., Machka C., Burracchi C., Bugarin C., Lopez-Granados E., del Pino Molina L., Campos-Guyotat L., Aanei C., Miguel J. F. S., Paiva B., Villamor-Casas N., Magnano L., Philippe J., Bonroy C., Denys B., Willems A., Breughe P., de Wolf J., Sousa A. E., and Silva S. L.

Abstract

Precise classification of acute leukemia (AL) is crucial for adequate treatment. EuroFlow has previously designed an AL orientation tube (ALOT) to guide toward the relevant classification panel and final diagnosis. In this study, we designed and validated an algorithm for automated (database-supported) gating and identification (AGI tool) of cell subsets within samples stained with ALOT. A reference database of normal peripheral blood (PB, n = 41) and bone marrow (BM; n = 45) samples analyzed with the ALOT was constructed, and served as a reference for the AGI tool to automatically identify normal cells. Populations not unequivocally identified as normal cells were labeled as checks and were classified by an expert. Additional normal BM (n = 25) and PB (n = 43) and leukemic samples (n = 109), analyzed in parallel by experts and the AGI tool, were used to evaluate the AGI tool. Analysis of normal PB and BM samples showed low percentages of checks (<3% in PB, <10% in BM), with variations between different laboratories. Manual analysis and AGI analysis of normal and leukemic samples showed high levels of correlation between cell numbers (r2 > 0.95 for all cell types in PB and r2 > 0.75 in BM) and resulted in highly concordant classification of leukemic cells by our previously published automated database-guided expert-supervised orientation tool for immunophenotypic diagnosis and classification of acute leukemia (Compass tool). Similar data were obtained using alternative, commercially available tubes, confirming the robustness of the developed tools. The AGI tool represents an innovative step in minimizing human intervention and requirements in expertise, toward a “sample-in and result-out” approach which may result in more objective and reproducible data analysis and diagnostics. The AGI tool may improve quality of immunophenotyping in individual laboratories, since high percentages of checks in normal samples are an alert on the quality of the internal p

Published
2021

9. Dynamics of broad H3K4me3 domains uncover an epigenetic switch between cell identity and cancer-related genes

Author

Belhocine, M., Simonin, Mathieu, Flores, Jose David Abad, Cieslak, Agata, Manosalva, Iris, Pradel, Lydie, Martens, Joost H.A., Stunnenberg, Hendrik G., Asnafi, V., Spicuglia, Salvatore, Belhocine, M., Simonin, Mathieu, Flores, Jose David Abad, Cieslak, Agata, Manosalva, Iris, Pradel, Lydie, Martens, Joost H.A., Stunnenberg, Hendrik G., Asnafi, V., and Spicuglia, Salvatore

Abstract

Contains fulltext : 252814.pdf (Publisher’s version ) (Closed access)

Published
2022

11. Prognostic value of PET/CT and CT in T‐cell lymphoblastic lymphoma/leukaemia patients: A retrospective cohort study of 145 patients.

Author

Rouzaud, C., Vercellino, L., De Kerviler, E., Raffoux, E., Balsat, M., Marcais, A., Dourthe, M.‐E., Meignin, V., Asnafi, V., MacIntyre, E., Boissel, N., and Lengliné, E.

Subjects
T-cell lymphoma, PROGNOSIS, LEUKEMIA, COHORT analysis, POSITRON emission tomography
Abstract

Details for diagnostic work-up, patient treatments, CT and PET/CT procedures, outcome criteria and statistical methods are outlined in the Supplementary Material. Since PET/CT has been demonstrated to be more sensitive and specific than CT for response evaluation,[7] we chose to analyse CT and PET/CT at diagnosis but only PET/CT for interim evaluation. Prognostic value of PET/CT and CT in T-cell lymphoblastic lymphoma/leukaemia patients: A retrospective cohort study of 145 patients Keywords: CT; PET/CT; T-cell acute lymphoblastic leukaemia; T-cell lymphoblastic lymphoma EN CT PET/CT T-cell acute lymphoblastic leukaemia T-cell lymphoblastic lymphoma e21 e24 4 04/20/23 20230501 NES 230501 T-cell acute lymphoblastic leukaemia (T-ALL) and lymphoma (T-LL) are malignancies arising from thymic T-cell precursors with heterogeneous tumor dissemination among bone marrow, blood and other organs. [Extracted from the article]

Published
2023
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12. Flow cytometry and IG/TCR quantitative PCR for minimal residual disease quantitation in acute lymphoblastic leukemia: a French multicenter prospective study on behalf of the FRALLE, EORTC and GRAALL

Author

Garand, R, Beldjord, K, Cavé, H, Fossat, C, Arnoux, I, Asnafi, V, Bertrand, Y, Boulland, M-L, Brouzes, C, Clappier, E, Delabesse, E, Fest, T, Garnache-Ottou, F, Huguet, F, Jacob, M-C, Kuhlein, E, Marty-Grès, S, Plesa, A, Robillard, N, Roussel, M, Tkaczuk, J, Dombret, H, Macintyre, E, Ifrah, N, Béné, M C, and Baruchel, A

Published
2013
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14. HIGH‐RISK MANTLE CELL LYMPHOMA IN THE LYMA TRIAL: A LYSA STUDY

Author

Cheminant, M., primary, Burroni, B., additional, Bris, Y., additional, Chartier, L., additional, Oberic, L., additional, Ribrag, V., additional, Delfau, M.‐H., additional, Thieblemont, C., additional, Gressin, R., additional, Canioni, D., additional, Peyre, M., additional, Laurent, C., additional, Steimle, T., additional, Kaltenbach, S., additional, Asnafi, V., additional, Macintyre, E., additional, Callanan, M., additional, Gouill, S., additional, and Hermine, O., additional

Published
2021
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15. A DL-4-and TNF alpha-based culture system to generate high numbers of nonmodified or genetically modified immunotherapeutic human T-lymphoid progenitors

Author

Moirangthem, R.D., Ma, K.Y., Lizot, S., Cordesse, A., Olivre, J., Chappedelaine, C. de, Joshi, A., Cieslak, A., Tchen, J., Cagnard, N., Asnafi, V., Rausell, A., Simons, L., Zuber, J., Taghon, T., Staal, F.J.T., Pflumio, F., Six, E., Cavazzana, M., Lagresle-Peyrou, C., Soheili, T., and Andre, I.

Subjects
EXPRESSION, RISK, Human T-lymphoid progenitor, Mobilized peripheral, ligand 4, Delta-like ligand 4, BLOOD, CELL TRANSPLANTATION, Tumor necrosis factor alpha, Hematopoietic stem and progenitor cells, HUMAN HEMATOPOIETIC STEM, PERIPHERAL-BLOOD, FAS, DELTA-LIKE 4, IMMUNE RECONSTITUTION, Mobilized peripheral blood, CORD, Medicine and Health Sciences, Delta-like, TUMOR-NECROSIS-FACTOR
Abstract

Several obstacles to the production, expansion and genetic modification of immunotherapeutic T cells in vitro have restricted the widespread use of T-cell immunotherapy. In the context of HSCT, delayed naive T-cell recovery contributes to poor outcomes. A novel approach to overcome the major limitations of both T-cell immunotherapy and HSCT would be to transplant human T-lymphoid progenitors (HTLPs), allowing reconstitution of a fully functional naive T-cell pool in the patient thymus. However, it is challenging to produce HTLPs in the high numbers required to meet clinical needs. Here, we found that adding tumor necrosis factor alpha (TNF alpha) to a DL-4-based culture system led to the generation of a large number of nonmodified or genetically modified HTLPs possessing highly efficient in vitro and in vivo T-cell potential from either CB HSPCs or mPB HSPCs through accelerated T-cell differentiation and enhanced HTLP cell cycling and survival. This study provides a clinically suitable cell culture platform to generate high numbers of clinically potent nonmodified or genetically modified HTLPs for accelerating immune recovery after HSCT and for T-cell-based immunotherapy (including CAR T-cell therapy).

Published
2021

16. Standardized MRD quantification in European ALL trials: Proceedings of the Second International Symposium on MRD assessment in Kiel, Germany, 18–20 September 2008

Author

Brüggemann, M, Schrauder, A, Raff, T, Pfeifer, H, Dworzak, M, Ottmann, O G, Asnafi, V, Baruchel, A, Bassan, R, Benoit, Y, Biondi, A, Cavé, H, Dombret, H, Fielding, A K, Foà, R, Gökbuget, N, Goldstone, A H, Goulden, N, Henze, G, Hoelzer, D, Janka-Schaub, G E, Macintyre, E A, Pieters, R, Rambaldi, A, Ribera, J-M, Schmiegelow, K, Spinelli, O, Stary, J, von Stackelberg, A, Kneba, M, Schrappe, M, and van Dongen, J J M

Published
2010
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19. CBFβ-SMMHC Affects Genome-wide Polycomb Repressive Complex 1 Activity in Acute Myeloid Leukemia

Author

Cordonnier, G., Mandoli, A., Cagnard, N., Hypolite, G., Lhermitte, L., Verhoeyen, E., Asnafi, V., Dillon, N., Macintyre, E., Martens, J.H.A., Bond, J., Cordonnier, G., Mandoli, A., Cagnard, N., Hypolite, G., Lhermitte, L., Verhoeyen, E., Asnafi, V., Dillon, N., Macintyre, E., Martens, J.H.A., and Bond, J.

Abstract

Contains fulltext : 227158.pdf (publisher's version ) (Open Access), Mutations and deletions of polycomb repressive complex (PRC) components are increasingly recognized to affect tumor biology in a range of cancers. However, little is known about how genetic alterations of PRC-interacting molecules such as the core binding …

Published
2020

20. Blueprint of human thymopoiesis reveals molecular mechanisms of stage-specific TCR enhancer activation

Author

Cieslak, Agata, Charbonnier, Guillaume, Tesio, Melania, Mathieu, Eve-Lyne, Belhocine, M., Touzart, Aurore, Martens, Joost H.A., Janssen-Megens, E.M., Stunnenberg, Hendrik G., Spicuglia, Salvatore, Asnafi, V., Cieslak, Agata, Charbonnier, Guillaume, Tesio, Melania, Mathieu, Eve-Lyne, Belhocine, M., Touzart, Aurore, Martens, Joost H.A., Janssen-Megens, E.M., Stunnenberg, Hendrik G., Spicuglia, Salvatore, and Asnafi, V.

Abstract

Contains fulltext : 225041.pdf (publisher's version ) (Open Access)

Published
2020

24. t(5;14)/HOX11L2-positive T-cell acute lymphoblastic leukemia. A collaborative study of the Groupe Français de Cytogénétique Hématologique (GFCH)

Author

Berger, R, Dastugue, N, Busson, M, van den Akker, J, Pérot, C, Ballerini, P, Hagemeijer, A, Michaux, L, Charrin, C, Pages, M P, Mugneret, F, Andrieux, J, Talmant, P, Hélias, C, Mauvieux, L, Lafage-Pochitaloff, M, Mozziconacci, M-J, Cornillet-Lefebvre, P, Radford, I, Asnafi, V, Bilhou-Nabera, C, Nguyen Khac, F, Léonard, C, Speleman, F, Poppe, B, Bastard, C, Taviaux, S, Quilichini, B, Herens, C, Grégoire, M-J, Cavé, H, and Bernard, O A

Published
2003
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25. Refractory coeliac sprue is a diffuse gastrointestinal disease. (Coeliac Disease)

Author

Verkarre, V., Asnafi, V., Lecomte, T., Mariaud-de Serre, N. Patey, Leborgne, M., Grosdidier, E., Bihan, C. Le, Macintyre, E., Cellier, C., Cerf-Bensussan, N., and Brousse, N.

Subjects
Celiac disease -- Research, T cell lymphoma -- Research, Health
Abstract

Background: Refractory coeliac sprue (RCS) with an immunophenotypically aberrant clonal intra epithelial lymphocyte (IEL) population is considered a cryptic form of intestinal T cell lymphoma. Aims: To investigate the distribution [...]

Published
2003

26. EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes

Author

van Dongen, J JM, Lhermitte, L, Böttcher, S, Almeida, J, van der Velden, V HJ, Flores-Montero, J, Rawstron, A, Asnafi, V, Lécrevisse, Q, Lucio, P, Mejstrikova, E, Szczepański, T, Kalina, T, de Tute, R, Brüggemann, M, Sedek, L, Cullen, M, Langerak, A W, Mendonça, A, Macintyre, E, Martin-Ayuso, M, Hrusak, O, Vidriales, M B, and Orfao, A

Published
2012
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28. Minimal residual disease (MRD) is a predictor of clinical outcome in elderly patients with MCL and identifies patients with long lasting remissions after R-CHOP or R-FC induction followed by maintenance with rituximab or IFN: first results of the randomized EU-MCL elderly trial: V115

Author

Pott, C., Delfau-Larue, M.-H., Beldjord, K., Böttcher, S., Macintyre, E., Asnafi, V., Siebert, R., Klapper, W., Unterhalt, M., Kneba, M., Hiddemann, W., Hermine, O., Kluin-Nelemans, H., Dreyling, M., and Hoster, E.

Published
2011

30. Automated database-guided expert-supervised orientation for immunophenotypic diagnosis and classification of acute leukemia

Author

Lhermitte, L., Mejstrikova, E., Sluijs-Gelling, A.J. van der, Grigore, G.E., Sedek, L., Bras, A.E., Gaipa, G., Costa, E.S. da, Novakova, M., Sonneveld, E., Buracchi, C., Bacelar, T.D., Marvelde, J.G.T., Trinquand, A., Asnafi, V., Szczepanski, T., Matarraz, S., Lopez, A., Vidriales, B., Bulsa, J., Hrusak, O., Kalina, T., Lecrevisse, Q., Ayuso, M.M., Bruggemann, M., Verde, J., Fernandez, P., Burgos, L., Paiva, B., Pedreira, C.E., Dongen, J.J.M. van, Orfao, A., Velden, V.H.J. van der, EuroFlow Consortium, Instituto de Salud Carlos III, Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil), Ministerio de Sanidad y Consumo (España), Polish Academy of Sciences, Fundações de Amparo à Pesquisa (Brasil), Ministerio de Economía y Competitividad (España), Immunology, Lhermitte, L, Mejstrikova, E, Van Der Sluijs-Gelling, A, Grigore, G, Sedek, L, Bras, A, Gaipa, G, Sobral Da Costa, E, Novakova, M, Sonneveld, E, Buracchi, C, De Sa Bacelar, T, Te Marvelde, J, Trinquand, A, Asnafi, V, Szczepanski, T, Matarraz, S, Lopez, A, Vidriales, B, Bulsa, J, Hrusak, O, Kalina, T, Lecrevisse, Q, Martin Ayuso, M, Bruggemann, M, Verde, J, Fernandez, P, Burgos, L, Paiva, B, Pedreira, C, Van Dongen, J, Orfao, A, and Van Der Velden, V

Subjects
Adult, Male, 0301 basic medicine, Cancer Research, Myeloid, Adolescent, T-cell acute lymphoblastic leukemia (T-ALL), Immunophenotyping, Young Adult, 03 medical and health sciences, Text mining, Acute leukemia (AL), EuroFlow, medicine, Humans, AL orientation tube (ALOT), Child, Aged, Aged, 80 and over, Acute leukemia, Leukemia, business.industry, Orientation (computer vision), Infant, Newborn, Infant, Myeloid leukemia, Pattern recognition, Hematology, Middle Aged, Precursor Cell Lymphoblastic Leukemia-Lymphoma, medicine.disease, Leukemia, Myeloid, Acute, Statistical classification, 030104 developmental biology, medicine.anatomical_structure, Oncology, Child, Preschool, Acute Disease, B-cell precursor (BCP), Female, Original Article, Artificial intelligence, business
Abstract

Precise classification of acute leukemia (AL) is crucial for adequate treatment. EuroFlow has previously designed an AL orientation tube (ALOT) to guide towards the relevant classification panel (T-cell acute lymphoblastic leukemia (T-ALL), B-cell precursor (BCP)-ALL and/or acute myeloid leukemia (AML)) and final diagnosis. Now we built a reference database with 656 typical AL samples (145 T-ALL, 377 BCP-ALL, 134 AML), processed and analyzed via standardized protocols. Using principal component analysis (PCA)-based plots and automated classification algorithms for direct comparison of single-cells from individual patients against the database, another 783 cases were subsequently evaluated. Depending on the database-guided results, patients were categorized as: (i) typical T, B or Myeloid without or; (ii) with a transitional component to another lineage; (iii) atypical; or (iv) mixed-lineage. Using this automated algorithm, in 781/783 cases (99.7%) the right panel was selected, and data comparable to the final WHO-diagnosis was already provided in >93% of cases (85% T-ALL, 97% BCP-ALL, 95% AML and 87% mixed-phenotype AL patients), even without data on the full-characterization panels. Our results show that database-guided analysis facilitates standardized interpretation of ALOT results and allows accurate selection of the relevant classification panels, hence providing a solid basis for designing future WHO AL classifications., The research was performed within the EuroFlow Consortium, which started with an EU-FP6 grant (LSHB-CT-2006-018708) and obtained sustainability by protecting and licensing intellectual property, thereby obtaining royalties, which are exclusively being used for supporting the EuroFlow research program (chairmen: JJMvD and AO). LS, JB and TS were supported by STRATEGMED3/304586/5/NCBR/2017 PersonALL grant of the Polish National Center for Research and Development. ESC acknowledges FAPERJ, Rio de Janeiro, Brazil (E26/110.105/2014; E26/102.191/2013) and Conselho Nacional de Desenvolvimento Científico e Tecnológico—CNPQ of Brazil (400194/2014-7). AO, SM and AL acknowledge the Instituto de Salud Carlos III (MINECO, Madrid, Spain) for the DTS15/00119, and CIBERONC-FEDER-CB16/12/00400 grants. EM, OH, TK and MN were supported by Ministry of Health grant number 15-28525A and NPU LO1604. The research for this manuscript was in part performed within the framework of the Erasmus Postgraduate School Molecular Medicine.

Published
2018

33. Blood monitoring of circulating tumor plasma cells by next generation flow in multiple myeloma after therapy

Author

Sanoja-Flores, L., Flores-Montero, J., Puig, N., Contreras-Sanfeliciano, T., Pontes, R., Corral-Mateos, A., Garcia-Sanchez, O., Diez-Campelo, M., Magalhaes, R.J.P. de, Garcia-Martin, L., Alonso-Alonso, J.M., Garcia-Mateo, A., Aguilar-Franco, C., Labrador, J., Barez-Garcia, A., Maiolino, A., Paiva, B., San Miguel, J., Costa, E.S. da, Gonzalez, M., Mateos, M.V., Durie, B., Dongen, J.J.M. van, Orfao, A., Bitter, W.M., Lubbers, B.R., Meij, A.S.M. van der, Teodosio, C.I., Zlei, M., Sluijs-Gelling, A.J. van der, Burg, M. van der, Velden, V.H.J. van der, Langerak, A.W., Marvelde, J. te, Schilperoord-Vermeulen, J., Blijkerk, A., Heezen, K.C., Almeida, J., Vidriales, M.B., Perez-Andres, J.F.M.M., Matarraz, S., Blanco, E., Martin, L., Lecrevisse, Q., Perez-Moran, J.J., Almeida, A.M., Silva, M.G. da, Faria, T., Bruggemann, M., Ritgen, M., Szczepanowski, M., Kohlscheen, S., Steinert, A., Harbst, E., Finke, J., Asnafi, V., Lhermitte, L., Duroyon, E., Trka, J., Hrusak, O., Kalina, T., Mejstrikova, E., Novakova, M., Thurner, D., Kanderova, V., Szczepanski, T., Sedek, L., Bulsa, J., Slota, L., Kulis, J., Pedreira, C.E., Nierkens, S., Jong, A. de, Koning, A. de, Lima, M., Santos, A.H., Bottcher, S., Lange, S., Engelmann, R., Paape, D., Machka, C., Gaipa, G., Burracchi, C., Bugarin, C., Lopez-Granados, E., Molina, L.D., Vlkova, M., Nechvatalova, J., Roussel, M., Campos-Guyotat, L., Aanei, C., Burgos, L., Villamor-Casas, N., Magnano, L., Philippe, J., Bonroy, C., Denys, B., Willems, A., Breughe, P., Wolf, J. de, Sousa, A.E., Silva, S.L., Fernandez, P., Morf, D., EuroFlow Consortium, and Immunology

Subjects
Pathology, medicine.medical_specialty, medicine.diagnostic_test, business.industry, Immunology, Plasma Cells, Cell Biology, Hematology, medicine.disease, Flow Cytometry, Neoplastic Cells, Circulating, Prognosis, Biochemistry, Flow cytometry, Immunophenotyping, Text mining, Flow (mathematics), medicine, Humans, business, Multiple Myeloma, Letter to Blood, Multiple myeloma
Published
2019

37. KIR3DL2 IS EXPRESSED IN PERIPHERAL T-CELL LYMPHOMAS AND MAY BE A THERAPEUTIC TARGET

Author

Cheminant, M., primary, Decroos, A., additional, Bruneau, J., additional, Carras, S., additional, Pelletier, L., additional, Martin, N., additional, Peri, V., additional, Guillot, F., additional, Paturel, C., additional, Sicard, H., additional, Bonnafous, C., additional, Lhermitte, L., additional, Molina, T., additional, Asnafi, V., additional, Genestier, L., additional, Gaulard, P., additional, Ortonne, N., additional, and Hermine, O., additional

Published
2019
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38. S131 IL7R EXPRESSION PREDICTS T-ALL SENSITIVITY TO JAK INHIBITORS REGARDLESS OF IL7R/JAK/STAT MUTATIONAL STATUS

Author

Kim, R., primary, Tueur, G., additional, Delecourt, M., additional, Boissel, N., additional, Feroul, M., additional, Latiri, M., additional, Vilarese, P., additional, Hippolyte, G., additional, Tesio, M., additional, Andrieu, G., additional, Ifrah, N., additional, Petit, A., additional, Dombret, H., additional, Macintyre, E., additional, Asnafi, V., additional, and Lhermitte, L., additional

Published
2019
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39. MEMBRANE EXPRESSION OF NK RECEPTOR KIR3DL2 CONTRIBUTES TO DELINEATE THE ACUTE-TYPE AND IS A THERAPEUTIC TARGET IN ADULT T-CELL LEUKEMIA/LYMPHOMA

Author

Cheminant, M., primary, Lhermitte, L., additional, Bruneau, J., additional, Sicard, H., additional, Bonnafous, C., additional, Ortonne, N., additional, Genestier, L., additional, Gaulard, P., additional, Palmic, P., additional, Dussiot, M., additional, Waast, L., additional, Avettand-Fenoel, V., additional, Brouzes, C., additional, Lepelletier, Y., additional, Asnafi, V., additional, Marçais, A., additional, and Hermine, O., additional

Published
2019
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40. Clinical aspects and outcome of lymphoblastic leukemia/lymphoma with cutaneous involvement

Author

Bontoux, C., primary, De Masson, A., additional, Boccara, O., additional, Bodemer, C., additional, Fraitag, S., additional, Balme, B., additional, Franck, N., additional, Carlotti, A., additional, Comoz, F., additional, Verneuil, L., additional, Brasme, J-F., additional, Duplan, M., additional, Croué, A., additional, Templier, I., additional, Beltraminelli, H., additional, Dereure, O., additional, Szablewski, V., additional, Thevenin, C., additional, Boulinguez, S., additional, Viraben, R., additional, Tournier, E., additional, Lamant, L., additional, Ortonne, N., additional, Ingen-Housz-Oro, S., additional, Beckerich, F., additional, Grange, F., additional, Durlach, A., additional, Amatore, F., additional, Frouin, E., additional, McIntyre, E., additional, Asnafi, V., additional, Kim, R., additional, Clappier, E., additional, Soulier, J., additional, Boissel, N., additional, Dombret, H., additional, Bagot, M., additional, and Battistella, M., additional

Published
2019
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42. Automated database-guided expert-supervised orientation for immunophenotypic diagnosis and classification of acute leukemia

Author

Lhermitte, L, Mejstrikova, E, Van Der Sluijs-Gelling, A, Grigore, G, Sedek, L, Bras, A, Gaipa, G, Sobral Da Costa, E, Novakova, M, Sonneveld, E, Buracchi, C, De Sa Bacelar, T, Te Marvelde, J, Trinquand, A, Asnafi, V, Szczepanski, T, Matarraz, S, Lopez, A, Vidriales, B, Bulsa, J, Hrusak, O, Kalina, T, Lecrevisse, Q, Martin Ayuso, M, Bruggemann, M, Verde, J, Fernandez, P, Burgos, L, Paiva, B, Pedreira, C, Van Dongen, J, Orfao, A, Van Der Velden, V, Lhermitte, L, Mejstrikova, E, Van Der Sluijs-Gelling, A, Grigore, G, Sedek, L, Bras, A, Gaipa, G, Sobral Da Costa, E, Novakova, M, Sonneveld, E, Buracchi, C, De Sa Bacelar, T, Te Marvelde, J, Trinquand, A, Asnafi, V, Szczepanski, T, Matarraz, S, Lopez, A, Vidriales, B, Bulsa, J, Hrusak, O, Kalina, T, Lecrevisse, Q, Martin Ayuso, M, Bruggemann, M, Verde, J, Fernandez, P, Burgos, L, Paiva, B, Pedreira, C, Van Dongen, J, Orfao, A, and Van Der Velden, V

Abstract

Precise classification of acute leukemia (AL) is crucial for adequate treatment. EuroFlow has previously designed an AL orientation tube (ALOT) to guide towards the relevant classification panel (T-cell acute lymphoblastic leukemia (T-ALL), B-cell precursor (BCP)-ALL and/or acute myeloid leukemia (AML)) and final diagnosis. Now we built a reference database with 656 typical AL samples (145 T-ALL, 377 BCP-ALL, 134 AML), processed and analyzed via standardized protocols. Using principal component analysis (PCA)-based plots and automated classification algorithms for direct comparison of single-cells from individual patients against the database, another 783 cases were subsequently evaluated. Depending on the database-guided results, patients were categorized as: (i) typical T, B or Myeloid without or; (ii) with a transitional component to another lineage; (iii) atypical; or (iv) mixed-lineage. Using this automated algorithm, in 781/783 cases (99.7%) the right panel was selected, and data comparable to the final WHO-diagnosis was already provided in >93% of cases (85% T-ALL, 97% BCP-ALL, 95% AML and 87% mixed-phenotype AL patients), even without data on the full-characterization panels. Our results show that database-guided analysis facilitates standardized interpretation of ALOT results and allows accurate selection of the relevant classification panels, hence providing a solid basis for designing future WHO AL classifications.

Published
2018

43. Automated database-guided expert-supervised orientation for immunophenotypic diagnosis and classification of acute leukemia

Author

Instituto de Salud Carlos III, Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil), Ministerio de Sanidad y Consumo (España), Polish Academy of Sciences, Fundações de Amparo à Pesquisa (Brasil), Ministerio de Economía y Competitividad (España), Lhermitte, Ludovic, Mejstrikova, Ester, Sluijs-Gelling, Alita J. van der, Grigore, Georgiana Emilia, Sedek, Lukasz, Bras, A. E., Gaipa, Giuseppe, Sobral da Costa, E., Novákova, Michaela, Sonneveld, Edwin, Buracchi, Chiara, Bacelar, Thiago S., Marvelde, Jeroen G. te, Trinquand, A., Asnafi, V., Szczepanski, Tomasz, Matarraz, Sergio, Lopez, A., Vidriales, Maria Belén, Bulsa, J., Hrusak, Ondrej, Kalina, Tomas, Lécrevisse, Quentin, Martín-Ayuso, Marta, Brüggemann, Monika, Verde, Javier, Fernandez, Paula, Burgos, Leire, Paiva, Bruno, Pedreira, C. E., Dongen, J. J. M. van, Orfao, Alberto, Velden, Vincent H. J. van der, Instituto de Salud Carlos III, Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil), Ministerio de Sanidad y Consumo (España), Polish Academy of Sciences, Fundações de Amparo à Pesquisa (Brasil), Ministerio de Economía y Competitividad (España), Lhermitte, Ludovic, Mejstrikova, Ester, Sluijs-Gelling, Alita J. van der, Grigore, Georgiana Emilia, Sedek, Lukasz, Bras, A. E., Gaipa, Giuseppe, Sobral da Costa, E., Novákova, Michaela, Sonneveld, Edwin, Buracchi, Chiara, Bacelar, Thiago S., Marvelde, Jeroen G. te, Trinquand, A., Asnafi, V., Szczepanski, Tomasz, Matarraz, Sergio, Lopez, A., Vidriales, Maria Belén, Bulsa, J., Hrusak, Ondrej, Kalina, Tomas, Lécrevisse, Quentin, Martín-Ayuso, Marta, Brüggemann, Monika, Verde, Javier, Fernandez, Paula, Burgos, Leire, Paiva, Bruno, Pedreira, C. E., Dongen, J. J. M. van, Orfao, Alberto, and Velden, Vincent H. J. van der

Abstract

Precise classification of acute leukemia (AL) is crucial for adequate treatment. EuroFlow has previously designed an AL orientation tube (ALOT) to guide towards the relevant classification panel (T-cell acute lymphoblastic leukemia (T-ALL), B-cell precursor (BCP)-ALL and/or acute myeloid leukemia (AML)) and final diagnosis. Now we built a reference database with 656 typical AL samples (145 T-ALL, 377 BCP-ALL, 134 AML), processed and analyzed via standardized protocols. Using principal component analysis (PCA)-based plots and automated classification algorithms for direct comparison of single-cells from individual patients against the database, another 783 cases were subsequently evaluated. Depending on the database-guided results, patients were categorized as: (i) typical T, B or Myeloid without or; (ii) with a transitional component to another lineage; (iii) atypical; or (iv) mixed-lineage. Using this automated algorithm, in 781/783 cases (99.7%) the right panel was selected, and data comparable to the final WHO-diagnosis was already provided in >93% of cases (85% T-ALL, 97% BCP-ALL, 95% AML and 87% mixed-phenotype AL patients), even without data on the full-characterization panels. Our results show that database-guided analysis facilitates standardized interpretation of ALOT results and allows accurate selection of the relevant classification panels, hence providing a solid basis for designing future WHO AL classifications.

Published
2018

44. KILT: A RANDOMIZED NON‐COMPARATIVE PHASE II LYSA STUDY OF LACUTAMAB WITH GEMOX VERSUS GEMOX IN RELAPSED/REFRACTORY PERIPHERAL T‐CELL LYMPHOMA.

Author

Cheminant, M., Carras, S., Bruneau, J., Lemonnier, F., Bachy, E., Herbaux, C., Feugier, P., Daguindau, N., Gastinne, T., Guillermin, Y., Lamarque, M., Slama, B., Wolfromm, A., Morschhauser, F., Tournilhac, O., Gaulard, P., Asnafi, V., Ortonne, N., Damaj, G., and Hermine, O.

Subjects
T-cell lymphoma, KILLER cells
Abstract

KILT: A RANDOMIZED NON-COMPARATIVE PHASE II LYSA STUDY OF LACUTAMAB WITH GEMOX VERSUS GEMOX IN RELAPSED/REFRACTORY PERIPHERAL T-CELL LYMPHOMA B Background: b Peripheral T-cell Lymphoma (PTCL) is a heterogeneous group of mature T-cell lymphomas (TCL) with adverse outcomes. KIR3DL2 is a killer immunoglobulin-like receptor that is expressed across different subtypes of TCL, including approximately 40% of PTCL. [Extracted from the article]

Published
2023
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45. CBF beta-SMMHC regulates ribosomal gene transcription and alters ribosome biogenesis

Author

Cordonnier, G., Mandoli, A., Radhouane, A., Hypolite, G., Lhermitte, L., Belhocine, M., Asnafi, V., Macintyre, E., Martens, J.H.A., Fumagalli, S., Bond, J., Cordonnier, G., Mandoli, A., Radhouane, A., Hypolite, G., Lhermitte, L., Belhocine, M., Asnafi, V., Macintyre, E., Martens, J.H.A., Fumagalli, S., and Bond, J.

Abstract

Contains fulltext : 175489.pdf (Publisher’s version ) (Closed access)

Published
2017

46. Direct interaction of Ikaros and Foxp1 modulates expression of the G protein-coupled receptor G2A in B-lymphocytes and acute lymphoblastic leukemia

Author

Bond J, Domaschenz R, Roman-Trufero M, Sabbattini P, Ferreiros-Vidal I, Gareth Gerrard, Asnafi V, Macintyre E, Merkenschlager M, and Dillon N

Subjects
Ikaros, acute leukemia, Foxp1, GPR132, B cell cycle
Abstract

Ikaros and Foxp1 are transcription factors that play key roles in normal lymphopoiesis and lymphoid malignancies. We describe a novel physical and functional interaction between the proteins, which requires the central zinc finger domain of Ikaros. The Ikaros-Foxp1 interaction is abolished by deletion of this region, which corresponds to the IK6 isoform that is commonly associated with high-risk acute lymphoblastic leukemia (ALL). We also identify the Gpr132 gene, which encodes the orphan G protein-coupled receptor G2A, as a novel target for Foxp1. Increased expression of Foxp1 enhanced Gpr132 transcription and caused cell cycle changes, including G2 arrest. Co-expression of wild-type Ikaros, but not IK6, displaced Foxp1 binding from the Gpr132 gene, reversed the increase in Gpr132 expression and inhibited G2 arrest. Analysis of primary ALL samples revealed a significant increase in GPR132 expression in IKZF1-deleted BCR-ABL negative patients, suggesting that levels of wild-type Ikaros may influence the regulation of G2A in B-ALL. Our results reveal a novel effect of Ikaros haploinsufficiency on Foxp1 functioning, and identify G2A as a potential modulator of the cell cycle in Ikaros-deleted B-ALL.

Published
2016

47. TCRα rearrangements identify a subgroup of NKL-deregulated adult T-ALLs associated with favorable outcome

Author

Villarese, P, primary, Lours, C, additional, Trinquand, A, additional, Le Noir, S, additional, Belhocine, M, additional, Lhermitte, L, additional, Cieslak, A, additional, Tesio, M, additional, Petit, A, additional, LeLorch, M, additional, Spicuglia, S, additional, Ifrah, N, additional, Dombret, H, additional, Langerak, A W, additional, Boissel, N, additional, Macintyre, E, additional, and Asnafi, V, additional

Published
2017
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48. NKP46 EXPRESSION IS A DIAGNOSTIC AND PROGNOSTIC BIOMARKER IN PRIMARY GASTROINTESTINAL T-CELL LYMPHOPROLIFERATIONS. A CELAC NETWORK STUDY

Author

Cheminant, M., primary, Bruneau, J., additional, Malamut, G., additional, Sibon, D., additional, Guegan, N., additional, Trinquand, A., additional, Lhermitte, L., additional, Brousse, N., additional, Suarez, F., additional, Frenzel, L., additional, Delarue, R., additional, Marçais, A., additional, Macintyre, E., additional, Asnafi, V., additional, Lhospice, F., additional, Bonnafous, C., additional, Molina, T., additional, Meresse, B., additional, Cellier, C., additional, Cerf-Bensussan, N., additional, and Hermine, O., additional

Published
2017
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50. Role of allogeneic stem cell transplantation in adult patients with Ph-negative acute lymphoblastic leukemia

Author

Dhedin, N, Huynh, A, Maury, S, Tabrizi, R, Beldjord, K, Asnafi, V, Thomas, X, Chevallier, P, Nguyen, S, Coiteux, V, Bourhis, J H, Hichri, Y, Escoffre-Barbe, M, Reman, O, Graux, C, Chalandon, Y, Blaise, D, Schanz, U, Lhéritier, V, Dombret, Hervé, Ifrah, N, University of Zurich, and Ifrah, N

Subjects
1307 Cell Biology, 2403 Immunology, 1303 Biochemistry, 10032 Clinic for Oncology and Hematology, 2720 Hematology, 610 Medicine & health
Published
2015

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