1. Transcriptome profiling of Ewing sarcomas – treatment resistance pathways and IGF‐dependency
- Author
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Yi Chen, Asle C. Hesla, Yingbo Lin, Mehran Ghaderi, Mingzhi Liu, Chen Yang, Yifan Zhang, Panagiotis Tsagkozis, Olle Larsson, and Felix Haglund
- Subjects
apoptosis ,Ewing sarcoma ,RNA‐seq ,IGF2 ,transcriptome profiling ,tumor progression ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Ewing sarcomas (ESs) are aggressive sarcomas driven by EWS fusion genes. We sought to investigate whether whole‐transcriptome sequencing (RNA‐seq) could be used to detect patterns associated with chemotherapy response or tumor progression after first‐line treatment. Transcriptome sequencing (RNA‐seq) of 13 ES cases was performed. Among the differentially expressed pathways, we identified IGF2 expression as a potential driver of chemotherapy response and progression. We investigated the effect of IGF2 on proliferation, radioresistance, apoptosis, and the transcriptome pattern in four ES cell lines and the effect of IGF2 expression in a validation series of 14 patients. Transcriptome analysis identified differentially expressed genes (adj. P
- Published
- 2020
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