24 results on '"Ashley A Hoffman"'
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2. The Impact of the Interim Leadership Experience on Library Middle Managers
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Ashley T. Hoffman and Amy E. G. Barker
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Public Administration ,Library and Information Sciences - Published
- 2022
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3. Student-Centered Design: Creating LibGuides Students Can Actually Use.
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Amy Barker and Ashley T. Hoffman
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- 2021
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4. Author response for 'Rocky Mountain forests are poised to recover following bark beetle outbreaks, but with altered composition'
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null Kyle C. Rodman, null Robert A. Andrus, null Amanda R. Carlson, null Trevor A. Carter, null Teresa B. Chapman, null Jonathan D. Coop, null Paula J. Fornwalt, null Nathan S. Gill, null Brian J. Harvey, null Ashley E. Hoffman, null Katharine C. Kelsey, null Dominik Kulakowski, null Daniel C. Laughlin, null Jenna E. Morris, null José F. Negrón, null Katherine M. Nigro, null Gregory S. Pappas, null Miranda D. Redmond, null Charles C. Rhoades, null Monique E. Rocca, null Zoe H. Schapira, null Jason S. Sibold, null Camille S. Stevens‐Rumann, null Thomas T. Veblen, null Jianmin Wang, null Xiaoyang Zhang, and null Sarah J. Hart
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- 2022
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5. Metaphylactic antimicrobial effects on occurrences of antimicrobial resistance in Salmonella enterica, Escherichia coli and Enterococcus spp. measured longitudinally from feedlot arrival to harvest in high-risk beef cattle
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Nathan S. Long, James E. Wells, Elaine D. Berry, Jerrad F. Legako, Dale R. Woerner, Guy H. Loneragan, Paul R. Broadway, Jeff A. Carroll, Nicole C. Burdick Sanchez, Samodha C. Fernando, Carley M. Bacon, Cory L. Helmuth, Taylor M. Smock, Jeff L. Manahan, Ashley A. Hoffman, and Kristin E. Hales
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Cattle Diseases ,Salmonella enterica ,General Medicine ,Applied Microbiology and Biotechnology ,Anti-Bacterial Agents ,Feces ,Anti-Infective Agents ,Salmonella ,Drug Resistance, Bacterial ,Escherichia coli ,Animals ,Cattle ,Enterococcus ,Escherichia coli Infections ,Biotechnology - Abstract
Aims Our objective was to determine how injectable antimicrobials affected populations of Salmonella enterica, Escherichia coli and Enterococcus spp. in feedlot cattle. Methods and Results Two arrival date blocks of high-risk crossbred beef cattle (n = 249; mean BW = 244 kg) were randomly assigned one of four antimicrobial treatments administered on day 0: sterile saline control (CON), tulathromycin (TUL), ceftiofur (CEF) or florfenicol (FLR). Faecal samples were collected on days 0, 28, 56, 112, 182 and study end (day 252 for block 1 and day 242 for block 2). Hide swabs and subiliac lymph nodes were collected the day before and the day of harvest. Samples were cultured for antimicrobial-resistant Salmonella, Escherichia coli and Enterococcus spp. The effect of treatment varied by day across all targeted bacterial populations (p ≤ 0.01) except total E. coli. Total E. coli counts were greatest on days 112, 182 and study end (p ≤ 0.01). Tulathromycin resulted in greater counts and prevalence of Salmonella from faeces than CON at study end (p ≤ 0.01). Tulathromycin and CEF yielded greater Salmonella hide prevalence and greater counts of 128ERYR E. coli at study end than CON (p ≤ 0.01). No faecal Salmonella resistant to tetracyclines or third-generation cephalosporins were detected. Ceftiofur was associated with greater counts of 8ERYR Enterococcus spp. at study end (p ≤ 0.03). By the day before harvest, antimicrobial use did not increase prevalence or counts for all other bacterial populations compared with CON (p ≥ 0.13). Conclusions Antimicrobial resistance (AMR) in feedlot cattle is not caused solely by using a metaphylactic antimicrobial on arrival, but more likely a multitude of environmental and management factors.
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- 2022
6. The effects of administering different metaphylactic antimicrobials on growth performance and health outcomes of high-risk, newly received feedlot steers
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Carley M Coppin, Taylor M Smock, Cory L Helmuth, Jeff L Manahan, Nathan S Long, Ashley A Hoffman, Jeffrey A Carroll, Paul R Broadway, Nicole C Burdick Sanchez, James E Wells, Samodha C Fernando, and Kristin E Hales
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General Veterinary ,Animal Science and Zoology - Abstract
Bovine respiratory disease (BRD) is the primary animal health concern facing feedlot producers. Many antimicrobial mitigation strategies are available, but few studies have compared feedlot performance during both the receiving and finishing periods following application of different antimicrobials used as metaphylaxis at arrival. The objective of this study was to compare antimicrobial metaphylaxis methods on clinical health and growth performance across both the receiving and finishing periods. A total of 238 multiple-sourced steers in two source blocks were used in a generalized complete block design. The four treatments included: 1) a negative control, 5 mL of sterile saline injected subcutaneously (CON); 2) subcutaneous administration of florfenicol at 40 mg/kg of BW (NUF); 3) subcutaneous administration of ceftiofur in the posterior aspect of the ear at 6.6 mg/kg of BW (EXC); and 4) subcutaneous administration of tulathromycin at 2.5 mg/kg of BW (DRA). The morbidity rate for the first treatment of BRD was decreased for the DRA and EXC treatments compared to CON and NUF (P
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- 2022
7. Sustaining and Enhancing the Scholarly Communications Department: A Comprehensive Guide. By Kris S. Helge, Ahmet Meti Tmava, and Amanda R. Zerangue. Santa Barbara, CA: Libraries Unlimited, 2020. Pp. vii+164. $70.00 (paper). ISBN: 978-1-4408-6699-9
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Ashley T. Hoffman
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Library and Information Sciences - Published
- 2021
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8. Effects of physical activity and feed and water restriction at reimplanting time on feed intake patterns, growth performance, and carcass characteristics of finishing beef steers
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Cory L, Helmuth, Dale R, Woerner, Michael A, Ballou, Jeff L, Manahan, Carley M, Coppin, Nathan S, Long, Ashley A, Hoffman, James Daniel, Young, Taylor M, Smock, and Kristin E, Hales
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General Veterinary ,Animal Science and Zoology - Abstract
In the feedlot, there can be a decrease in dry matter intake (DMI) associated with reimplanting cattle that negatively affects growth performance. This study was conducted to determine the mechanisms causing a decrease in DMI after reimplanting and identify a strategy to mitigate the decrease. Crossbred steers (n = 200; 10 pens/treatment; initial bodyweight [BW] = 386 ± 4.9 kg) were used in a randomized complete block design experiment. Cattle were implanted with Revalor-IS on day 0. Treatments included a Revalor-200 implant on day 90 before feeding with the following management practices imposed: 1) steers were returned to their home pen immediately after reimplant (PCON); 2) steers were placed in pens and restricted from feed and water for 4 h (RES); 3) steers were walked an additional 805 m after reimplant and then returned home (LOC); 4) steers were restricted from feed and water for 4 h and walked an additional 805 m (RES + LOC); 5) steers were given an oral bolus of Megasphaera elsdenii (Lactipro; MS Biotec, Wamego, KS) and were restricted from feed and water for 4 h, and then walked an additional 805 m (LACT). One hundred steers were given an ear tag to record minutes of activity (ESense Flex Tags, Allflex Livestock Intelligence, Madison, WI). As a percentage of BW, DMI was 5% greater (P = 0.01) from reimplant to end for PCON vs. RES, LOC, and RES + LOC treatments. Likewise, as a percentage of BW, DMI was 6.6% greater (P = 0.03) from reimplant to end and 4.0% greater (P = 0.05) overall for the PCON treatment vs. the LOC treatment. Overall, DMI as a percentage of BW was 3.3% greater (P = 0.02) for PCON vs. RES, LOC, and RES + LOC treatments. There was an increase in G:F from reimplant to end (P = 0.05) for RES + LOC vs. the LACT treatment. From these data, we conclude that restricting cattle from feed and water for 4 h after reimplanting did not alter subsequent DMI. Increasing locomotion had the greatest negative effect on DMI and growth performance. Management strategies to decrease locomotion associated with reimplanting would be beneficial to DMI and overall growth performance of finishing beef steers.
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- 2022
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9. Partnering Against Poverty: Fighting POSH Bias Through Increased Interdisciplinary Research and Practice
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Morrie Mullins, Laura Sywulak, Shujaat F. Ahmed, and Ashley J. Hoffman
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Low income ,Carr ,020205 medical informatics ,Social Psychology ,Conceptualization ,Poverty ,Informal sector ,business.industry ,05 social sciences ,02 engineering and technology ,Public relations ,0502 economics and business ,0202 electrical engineering, electronic engineering, information engineering ,Industrial and organizational psychology ,business ,050203 business & management ,Applied Psychology - Abstract
Industrial-organizational (I-O) psychology is indeed moving forward in its involvement in humanitarian concerns (Berry et al., 2011), but as Gloss, Carr, Reichman, Abdul-Nasiru, and Oestereich (2017) point out, I-O psychologists tend to focus less on those of low income and the informal economy and more on working professionals in the formal economy (POSH). We propose (a) additional reasons for why the POSH bias may undermine science, (b) more solutions to benefit the impoverished, and (c) a broader conceptualization of humanitarian work psychology (HWP).
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- 2017
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10. Student-Centered Design: Creating LibGuides Students Can Actually Use
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Ashley T. Hoffman and Amy E. G. Barker
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Structure (mathematical logic) ,Research design ,Computer science ,business.industry ,Subject (documents) ,Usability ,Library and Information Sciences ,Library instruction ,Card sorting ,Blueprint ,ComputingMilieux_COMPUTERSANDEDUCATION ,Mathematics education ,Library classification ,business - Abstract
In this mixed-methods study, librarians at Kennesaw State University Library System conducted a year-long design research project to create a flexible subject guide “blueprint” for undergraduate students using LibGuides. Methods included a card sorting study with 18 undergraduate students and usability testing with 40 undergraduate students. The study’s goals were to identify what content, aesthetic design, organization, and structure students preferred on a subject guide. This paper addresses the current literature on research guides usability, overviews the design and implementation of the study, and highlights practical results that will easily be transferrable to other libraries.
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- 2021
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11. Humanitarian Work Psychology and the Global Development Agenda: Case Studies and Interventions Ishbel McWha-Hermann , Douglas C. Maynard and Mary O'Neill Berry (eds) *
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Drew B. Mallory and Ashley J. Hoffman
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Psychoanalysis ,Sociology and Political Science ,Law ,Psychological intervention ,Sociology ,Industrial and organizational psychology ,International development - Published
- 2016
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12. The 'How' Matters as Much as the 'Who'
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Ashley J. Hoffman
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Carr ,Social Psychology ,business.industry ,Humanitarian aid ,media_common.quotation_subject ,05 social sciences ,Subject (philosophy) ,050109 social psychology ,Public relations ,Humanism ,Work (electrical) ,0502 economics and business ,0501 psychology and cognitive sciences ,Bureaucracy ,business ,050203 business & management ,Applied Psychology ,Front (military) ,media_common - Abstract
It appears that although Gloss, Carr, Reichman, Abdul-Nasiru, and Oestereich (2017) have considered many of the arenas where industrial and organizational (I-O) psychology intersects with impoverished or atypical populations, an oft-overlooked domain is that of I-O psychologists working in military settings. Arguably the largest humanitarian aid and development organization in the world, national militaries offer a rich opportunity for I-O psychologists to study not only the more “POSH” aspects of work, but also the difficulties surrounding employees who must address impoverished citizens, lack of resources, bureaucracy far removed from the front lines, discrimination, threats to safety, and all kinds of cross-cultural interactions. As such, this humanistic, humanitarian movement could do well from studying the ways military units have successfully and not-so-successfully approached a timely and difficult subject.
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- 2017
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13. Revitalizing your library faculty governance: Five tips to increase involvement
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Ashley T. Hoffman
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Service (business) ,business.industry ,media_common.quotation_subject ,Corporate governance ,Academic freedom ,Library and Information Sciences ,Public relations ,Education ,Scholarship ,State (polity) ,Political science ,Institution ,business ,media_common - Abstract
If you’re an academic librarian with faculty status, you know that this status comes with an equal portion of benefit and burden. Some of the benefits are academic freedom, support for scholarship, and elevated status on campus (though not necessarily higher pay). Some library faculty are even eligible for tenure (though at my institution, Kennesaw State University, we are not). On the flipside of these benefits are a few things I would consider burdens, such as tedious annual reviews and extensive service requirements. Library faculty governance, I would argue, falls somewhere in between a benefit and a burden.
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- 2020
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14. List of Contributors
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Redzuan Abdullah, Joanna M. Anderson, Kylie Bailin, Ariana Baker, Leah C. Banks, Anne C. Behler, Veronica Bielat, Bill Blair, Eleni Borompoka, David Boudinot, Lindsay Bush, Dawn (Nikki) Cannon-Rech, Ashley J. Cole, Cynthia H. Comer, Lorna M. Dawes, Wendy C. Doucette, Ashley England, Nicole Eva, Kayla Flegal, Janice Galloway, Megan Gilpin, Gail Golderman, Crystal Goldman, Jamie L. Goodfellow, Stephanie J. Graves, Jessica Hagman, Justin Harrison, Tiffany Hebb, Sophie Herbert, Megan Hodge, Ashley T. Hoffman, Christina Holm, Meggan Houlihan, Daniel Ireton, Benjamin Jahre, Genevieve A. Jones-Edman, Matthew LaBrake, Sarah LeMire, Jessica C. Lewis, Beth Daniel Lindsay, Darchelle Martin, Jemima McDonald, Zackary Chance Medlin, Kathryn C. Millis, Stephanie Miranda, Sarah Morris, Rajen Munoo, Trenia Napier, Vicki M. Palmer, Julie Piacentine, Amanda Piekart-Primiano, Charissa Powell, Robyn Reed, Matthew T. Regan, Steven Remenapp, Quemar Rhoden, Emily Rimland, Caron Rollins, Ian Rossiter, Amanda Roth, Ingrid Ruffin, Fiona Salisbury, Courtney Seymour, Catherine Silvers, Kaitlin Springmier, Ashley Stark, Rebecca Starkey, Zita Szabo, Sarah Thorngate, Rebecca L. Tolley, Leah Townsend, Dominique Turnbow, Eng Ung, Ellen R. Urton, Jennifer L.A. Whelan, Laura L. Wilson, and George Zedan
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- 2018
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15. Coming to a Screen Near You
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Ashley T. Hoffman and Christina Holm
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- 2018
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16. Two castes sizes of leafcutter ants in task partitioning in foraging activity
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Carlos Arturo Navas Iannini, Pedro Ribeiro, Ashley N. Hoffman, Marcelo Toledo, João Vitor Tomotani, André Frazão Helene, Halee Rachel Watel, Priscilla Shiota Fedichina Carrossoni, and Daniella Klebaner
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0106 biological sciences ,0301 basic medicine ,Atta ,General Veterinary ,lcsh:S ,Biology ,biology.organism_classification ,01 natural sciences ,lcsh:S1-972 ,lcsh:Agriculture ,010602 entomology ,03 medical and health sciences ,ecological adaptation ,030104 developmental biology ,leaf cutting ,EVOLUÇÃO ,Correlation analysis ,Ant behavior ,Animal Science and Zoology ,lcsh:Agriculture (General) ,Agronomy and Crop Science ,Humanities ,ant behavior - Abstract
Particionamento de tarefas em animais eusociais e provavelmente uma adaptacao evolutiva, que otimiza a eficiencia de forrageio da colonia, gerando maior capacidade de crescimento e reproducao. Nos investigamos o particionamento indireto de tarefas, com o envolvimento de castas de dois tamanhos distintos. Assim, o material coletado por uma formiga nao era transportado diretamente ao ninho, nem transferido diretamente de um individuo para outro, mas sim descartado por uma formiga e coletado por outra. Em duas abordagens distintas, nos confirmamos em Atta sexdens rubropilosa resultados anteriores relativos a ocorrencia deste particionamento indireto de tarefas, observado em Atta colombica . Mostramos tambem que, assim como em Atta colombica , ha uma correlacao entre os tamanhos da formiga e do fragmento de folha transportado quando esta corta e transporta este fragmento ao ninho (CaT). Mais ainda, confirmamos em Atta sexdens que, quando o particionamento de tarefas ocorre e as formigas apenas transportam o fragmento de folhas (T) cortadas por outras formigas, a correlacao desaparece. Nos tambem descrevemos que as formigas CaT sao menores do que as T.
- Published
- 2016
17. Analysis of polyethylene glycol (PEG) fusion in cultured neuroblastoma cells via flow cytometry: Techniquesoptimization
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Ashley N. Hoffman, Wesley P. Thayer, Alonda C. Pollins, and Ravinder Bamba
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0301 basic medicine ,macromolecular substances ,Polyethylene glycol ,Flow cytometry ,Polyethylene Glycols ,Neuroblastoma cell ,Cell Fusion ,03 medical and health sciences ,chemistry.chemical_compound ,Neuroblastoma ,0302 clinical medicine ,Physiology (medical) ,Cell Line, Tumor ,PEG ratio ,medicine ,Animals ,Rat Neuroblastoma ,Neurons ,Fusion ,Cell fusion ,medicine.diagnostic_test ,business.industry ,technology, industry, and agriculture ,General Medicine ,Anatomy ,Flow Cytometry ,Rats ,030104 developmental biology ,Membrane ,Neurology ,chemistry ,Biophysics ,Surgery ,Neurology (clinical) ,business ,030217 neurology & neurosurgery - Abstract
Polyethylene glycol (PEG) has long been used as a membrane fusogen, but recently it has been adopted as a technique for peripheral nerve repair. Vertebrate models using PEG fusion have shown improved outcomes when PEG is applied during repair of severed peripheral nerves. The cellular mechanism of PEG fusion in the peripheral nerve repair model has not previously been assessed via flow cytometry. PEG fusion was assessed in this experiment by dying B35 rat neuroblastoma cells with different color fluorescent labels. The different color cells were combined and PEG was applied in concentrations of 50%, 75% and 100%. The amount of cell fusion was assessed via flow cytometry as the percentage of double positive cells. Results showed increasing fusion and decreasing viability with increasing concentrations of PEG.
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- 2016
18. Flap Reconstruction for Pressure Ulcers
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Lillian B. Nanney, James J. Madden, Ravinder Bamba, Marcia Spear, Wesley P. Thayer, Justine S. Kim, and Ashley N. Hoffman
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2. Zero hunger ,medicine.medical_specialty ,business.industry ,MEDLINE ,Outcome analysis ,030230 surgery ,Multiple risk factors ,3. Good health ,Surgery ,03 medical and health sciences ,0302 clinical medicine ,Text mining ,030220 oncology & carcinogenesis ,Medicine ,Original Article ,business ,Complication - Abstract
Background: Historically, complication rates after pressure ulcer reconstruction utilizing flap coverage have been high. Patients undergoing operations for pressure ulcer coverage typically have multiple risk factors for postoperative complications. The purpose of this study was to examine a large patient series in the pressure ulcer population to uncover objective evidence of the linkage between risk factors and outcomes after flap coverage. Methods: This study was a retrospective chart review of patients who underwent flap reconstruction for a pressure ulcer between 1997 and 2015. The characteristics of patients were analyzed to determine those who had complications such as pressure ulcer recurrence, wound dehiscence, and wound infection. Results: All patients (N = 276) underwent flap coverage of their pressure ulcers. The overall complication rate was 58.7% (162 patients). Wound dehiscence was the most common complication (31.2%), and the pressure ulcer recurrence rate was 28.6%. Multivariate regression for pressure ulcer recurrence revealed that body mass index
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- 2017
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19. Two castes sizes of leafcutter ants in task partitioning in foraging activity
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Marcelo Arruda de Toledo, Pedro Leite Ribeiro, Priscilla Shiota Fedichina Carrossoni, João Vitor Tomotani, Ashley Nicole Hoffman, Daniella Klebaner, Halee Rachel Watel, Carlos Arturo Navas Iannini, and André Frazão Helene
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Atta ,ant behavior ,leaf cutting ,ecological adaptation ,Agriculture ,Agriculture (General) ,S1-972 - Abstract
ABSTRACT: Task partitioning in eusocial animals is most likely an evolutionary adaptation that optimizes the efficiency of the colony to grow and reproduce. It was investigated indirect task partitioning in two castes sizes; this involves task partitioning in which the material transported is not transferred directly from one individual to another, but where it is dropped by one ant to be picked up by another. In two separate approaches, it was confirmed previous results pertaining to leaf caching activities among Atta colombica with task partitioning activities involving leaf dropping among Atta sexdens rubropilosa , in which there is a correlation between the size of an individual ant and the leaf fragment it transports. It was also suggested that this correlation exists only in individual ants that cut and transport (CaT) the same fragment to the nest. When task partitioning occurs and individual ants transporting (T) leaf fragments cut by other ants, the correlation becomes looser or disappears. We also observed that CaT ants are smaller than T ants.
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20. Policy, Systems, and Environmental Changes in Child Nutrition Programs: A Systematic Literature Review.
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Palmer S, Burton-Obanla A, Goon S, Allison T, Mitchell A, Bogdonas K, Fombelle M, Hoffman A, Smith J, McCaffrey J, and Prescott MP
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- Child, Humans, United States, Fruit, Vegetables, Lunch, Water, Nutrition Policy, Food Preferences, Child Nutritional Physiological Phenomena, Food Services
- Abstract
The National School Lunch Program (NSLP) provides healthy food to millions of children annually. To promote increased lunch consumption, policy, systems, and environmental (PSE) change strategies are being implemented in child nutrition programs. An evaluation of the current evidence supporting PSE interventions in school nutrition programs is needed to facilitate evidence-based practices across the nation for programs. This systematic review aims to determine the quality and breadth of available evidence of the effectiveness of PSE strategies on the consumption and waste of fruits, vegetables, milk, and water in the NSLP. The inclusion criteria required studies to occur in a United States K-12 school setting, data collection after 2012, report consumption and waste findings for fruit, vegetable, milk, or water, and be an original research article. Articles included in the review are restricted to positive or neutral quality. Thirty studies are included, policy level (n = 4), systems level (n = 8), environmental level (n = 10), and multi-category (n = 8). Results from positively rated policy-level studies suggest that recess before lunch may increase milk consumption, whereas removing flavored milk may decrease consumption. System-level studies of offering vegetables first in isolation of other meal components and offering spiced vegetables compared with traditional preparations may increase vegetable consumption, and locally procuring produce may increase fruit and vegetable consumption. Environmental-level studies such as water promotion strategies such as placing cups near drinking fountains may increase water consumption. Improving the convenience, attractiveness, and palatability of fruits and vegetables may increase consumption. Future PSE research in child nutrition programs should incorporate implementation aides and metrics into their study designs to allow a better understanding of how to sustain interventions from the perspective of school nutrition professionals., (Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.)
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- 2024
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21. Inflammatory Activity of Epithelial Stem Cell Variants from Cystic Fibrosis Lungs Is Not Resolved by CFTR Modulators.
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Wang S, Niroula S, Hoffman A, Khorrami M, Khorrami M, Yuan F, Gasser GN, Choi S, Liu B, Li J, Metersky ML, Vincent M, Crum CP, Boucher RC, Karmouty-Quintana H, Huang HJ, Sheshadri A, Dickey BF, Parekh KR, Engelhardt JF, McKeon FD, and Xian W
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- Humans, Child, Preschool, Animals, Mice, Cystic Fibrosis Transmembrane Conductance Regulator metabolism, Lung pathology, Inflammation metabolism, Cystic Fibrosis drug therapy, Cystic Fibrosis genetics, Cystic Fibrosis metabolism, Pulmonary Disease, Chronic Obstructive pathology
- Abstract
Rationale: CFTR (cystic fibrosis transmembrane conductance regulator) modulator drugs restore function to mutant channels in patients with cystic fibrosis (CF) and lead to improvements in body mass index and lung function. Although it is anticipated that early childhood treatment with CFTR modulators will significantly delay or even prevent the onset of advanced lung disease, lung neutrophils and inflammatory cytokines remain high in patients with CF with established lung disease despite modulator therapy, underscoring the need to identify and ultimately target the sources of this inflammation in CF lungs. Objectives: To determine whether CF lungs, like chronic obstructive pulmonary disease (COPD) lungs, harbor potentially pathogenic stem cell "variants" distinct from the normal p63/Krt5 lung stem cells devoted to alveolar fates, to identify specific variants that might contribute to the inflammatory state of CF lungs, and to assess the impact of CFTR genetic complementation or CFTR modulators on the inflammatory variants identified herein. Methods: Stem cell cloning technology developed to resolve pathogenic stem cell heterogeneity in COPD and idiopathic pulmonary fibrosis lungs was applied to end-stage lungs of patients with CF (three homozygous CFTR:F508D, one CFTR F508D/L1254X; FEV
1 , 14-30%) undergoing therapeutic lung transplantation. Single-cell-derived clones corresponding to the six stem cell clusters resolved by single-cell RNA sequencing of these libraries were assessed by RNA sequencing and xenografting to monitor inflammation, fibrosis, and mucin secretion. The impact of CFTR activity on these variants after CFTR gene complementation or exposure to CFTR modulators was assessed by molecular and functional studies. Measurements and Main Results: End-stage CF lungs display a stem cell heterogeneity marked by five predominant variants in addition to the normal lung stem cell, of which three are proinflammatory both at the level of gene expression and their ability to drive neutrophilic inflammation in xenografts in immunodeficient mice. The proinflammatory functions of these three variants were unallayed by genetic or pharmacological restoration of CFTR activity. Conclusions: The emergence of three proinflammatory stem cell variants in CF lungs may contribute to the persistence of lung inflammation in patients with CF with advanced disease undergoing CFTR modulator therapy.- Published
- 2023
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22. Cloning a profibrotic stem cell variant in idiopathic pulmonary fibrosis.
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Wang S, Rao W, Hoffman A, Lin J, Li J, Lin T, Liew AA, Vincent M, Mertens TCJ, Karmouty-Quintana H, Crum CP, Metersky ML, Schwartz DA, Davies PJA, Stephan C, Jyothula SSK, Sheshadri A, Suarez EE, Huang HJ, Engelhardt JF, Dickey BF, Parekh KR, McKeon FD, and Xian W
- Subjects
- Humans, Lung pathology, Myofibroblasts pathology, Fibroblasts pathology, Stem Cells metabolism, Cloning, Molecular, Idiopathic Pulmonary Fibrosis genetics, Idiopathic Pulmonary Fibrosis pathology
- Abstract
Idiopathic pulmonary fibrosis (IPF) is a progressive, irreversible, and rapidly fatal interstitial lung disease marked by the replacement of lung alveoli with dense fibrotic matrices. Although the mechanisms initiating IPF remain unclear, rare and common alleles of genes expressed in lung epithelia, combined with aging, contribute to the risk for this condition. Consistently, single-cell RNA sequencing (scRNA-seq) studies have identified lung basal cell heterogeneity in IPF that might be pathogenic. We used single-cell cloning technologies to generate "libraries" of basal stem cells from the distal lungs of 16 patients with IPF and 10 controls. We identified a major stem cell variant that was distinguished from normal stem cells by its ability to transform normal lung fibroblasts into pathogenic myofibroblasts in vitro and to activate and recruit myofibroblasts in clonal xenografts. This profibrotic stem cell variant, which was shown to preexist in low quantities in normal and even fetal lungs, expressed a broad network of genes implicated in organ fibrosis and showed overlap in gene expression with abnormal epithelial signatures identified in previously published scRNA-seq studies of IPF. Drug screens highlighted specific vulnerabilities of this profibrotic variant to inhibitors of epidermal growth factor and mammalian target of rapamycin signaling as prospective therapeutic targets. This profibrotic stem cell variant in IPF was distinct from recently identified profibrotic stem cell variants in chronic obstructive pulmonary disease and may extend the notion that inappropriate accrual of minor and preexisting stem cell variants contributes to chronic lung conditions.
- Published
- 2023
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23. Reduced fire severity offers near-term buffer to climate-driven declines in conifer resilience across the western United States.
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Davis KT, Robles MD, Kemp KB, Higuera PE, Chapman T, Metlen KL, Peeler JL, Rodman KC, Woolley T, Addington RN, Buma BJ, Cansler CA, Case MJ, Collins BM, Coop JD, Dobrowski SZ, Gill NS, Haffey C, Harris LB, Harvey BJ, Haugo RD, Hurteau MD, Kulakowski D, Littlefield CE, McCauley LA, Povak N, Shive KL, Smith E, Stevens JT, Stevens-Rumann CS, Taylor AH, Tepley AJ, Young DJN, Andrus RA, Battaglia MA, Berkey JK, Busby SU, Carlson AR, Chambers ME, Dodson EK, Donato DC, Downing WM, Fornwalt PJ, Halofsky JS, Hoffman A, Holz A, Iniguez JM, Krawchuk MA, Kreider MR, Larson AJ, Meigs GW, Roccaforte JP, Rother MT, Safford H, Schaedel M, Sibold JS, Singleton MP, Turner MG, Urza AK, Clark-Wolf KD, Yocom L, Fontaine JB, and Campbell JL
- Subjects
- Climate, Climate Change, Fires, Wildfires, Tracheophyta
- Abstract
Increasing fire severity and warmer, drier postfire conditions are making forests in the western United States (West) vulnerable to ecological transformation. Yet, the relative importance of and interactions between these drivers of forest change remain unresolved, particularly over upcoming decades. Here, we assess how the interactive impacts of changing climate and wildfire activity influenced conifer regeneration after 334 wildfires, using a dataset of postfire conifer regeneration from 10,230 field plots. Our findings highlight declining regeneration capacity across the West over the past four decades for the eight dominant conifer species studied. Postfire regeneration is sensitive to high-severity fire, which limits seed availability, and postfire climate, which influences seedling establishment. In the near-term, projected differences in recruitment probability between low- and high-severity fire scenarios were larger than projected climate change impacts for most species, suggesting that reductions in fire severity, and resultant impacts on seed availability, could partially offset expected climate-driven declines in postfire regeneration. Across 40 to 42% of the study area, we project postfire conifer regeneration to be likely following low-severity but not high-severity fire under future climate scenarios (2031 to 2050). However, increasingly warm, dry climate conditions are projected to eventually outweigh the influence of fire severity and seed availability. The percent of the study area considered unlikely to experience conifer regeneration, regardless of fire severity, increased from 5% in 1981 to 2000 to 26 to 31% by mid-century, highlighting a limited time window over which management actions that reduce fire severity may effectively support postfire conifer regeneration.
- Published
- 2023
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24. Implementation of a Multi-Component School Lunch Environmental Change Intervention to Improve Child Fruit and Vegetable Intake: A Mixed-Methods Study.
- Author
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Hamdi N, Ellison B, McCaffrey J, Metcalfe JJ, Hoffman A, Haywood P, and Prescott MP
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- Child, Female, Food Preferences, Fruit, Humans, Male, Schools, Vegetables, Food Services, Lunch
- Abstract
Nudge interventions are widely used to promote health in schools, yet implementation metrics are seldom used to understand intervention outcomes. A multi-component intervention consisting of cafeteria decorations, creative names, social norming taste tests, and flavor station components was implemented in three rural elementary school cafeterias by school nutrition services (SNS) and extension staff. Selection and consumption of fruits and vegetables at lunch were measured through monthly plate waste assessments over eight months ( n = 1255 trays). Interviews were conducted with SNS staff ( n = 3) upon completion of the intervention to assess implementation outcomes using validated acceptability and feasibility metrics. Consumption findings were generally inconsistent across schools and time points, yet fruit consumption increased at School 1 ( p < 0.05) during the taste test and flavor station intervention months and School 2 ( p < 0.001) during the creative names intervention months compared to baseline. Odds of selecting a vegetable at School 3 were three times higher than baseline during the taste test intervention months (odds ratio (OR), 3.0; 95% confidence interval (CI), 1.3-6.5). Cafeteria decorations and taste tests had higher reported implementation metrics for acceptability and feasibility than other interventions. Thematic analysis underscored the facilitating role of extension support, as well as systems factors, which served as facilitators and barriers across schools and interventions. These findings suggest that nudge interventions are a promising strategy to improve vegetable selection and fruit consumption in school meal programs.
- Published
- 2020
- Full Text
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