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14 results on '"Ashimori, Atsushige"'

3. HIF-1α-dependent upregulation of angiogenic factors by mechanical stimulation in retinal pigment epithelial cells

Author

Ashimori, Atsushige, primary, Higashijima, Fumiaki, additional, Ogata, Tadahiko, additional, Sakuma, Ayano, additional, Hamada, Waka, additional, Sunada, Junki, additional, Aoki, Ren, additional, Mikuni, Masanori, additional, Hayashi, Ken'ichiro, additional, Wakuta, Makiko, additional, Yoshimoto, Takuya, additional, Minamoto, Akira, additional, Ko, Ji-Ae, additional, and Kimura, Kazuhiro, additional

Published
2024
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4. Multi‐Electrode Electroretinography with Transparent Microelectrodes Printed on a Soft and Wet Contact Lens.

Author

Hu, Lunjie, Azhari, Saman, Li, Qianyu, Zhang, Hanzhe, Ashimori, Atsushige, Kimura, Kazuhiro, and Miyake, Takeo

Subjects
SOFT contact lenses, ELECTRORETINOGRAPHY, MICROELECTRODES, POLYMER electrodes, CELL survival
Abstract

Visual electrophysiology measurements are crucial in ophthalmology as they are frequently used for diagnosing and treating numerous ocular diseases. Therefore, optically transparent and flexible electrodes with high sensitivity at localized regions are in high demand. Here, it is aimed to develop a soft, transparent, multi‐electrode system assembled on commercially available soft contact lenses and assess its application in measuring Electroretinography (ERG) responses in rabbits. A biocompatible multi‐electrode system on commercial disposable soft contact lenses is successfully constructed, showing 95% cell viability, 82% optical transparency over the visible range, and good flexibility at 10% strain. Direct current (DC) voltage is used to produce a highly resistive overoxidized poly(3,4‐ethylenedioxythiophene) (PEDOT) layer that covers all parts of the electrodes except the sensing region, which, owing to its high conductivity, can accurately measure electrical signals from local regions of the cornea. Spatially resolved ERG recordings of rabbits are obtained with superior signal quality using this multi‐electrode system. Thus, this device is expected to be used in ophthalmic diagnosis in the future. [ABSTRACT FROM AUTHOR]

Published
2024
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8. Attenuated SIRT1 Activity Leads to PER2 Cytoplasmic Localization and Dampens the Amplitude of Bmal1 Promoter-Driven Circadian Oscillation

Author

Ashimori, Atsushige, Nakahata, Yasukazu, Sato, Toshiya, Fukamizu, Yuichiro, Matsui, Takaaki, Yoshitane, Hikari, Fukada, Yoshitaka, Shinohara, Kazuyuki, Bessho, Yasumasa, Ashimori, Atsushige, Nakahata, Yasukazu, Sato, Toshiya, Fukamizu, Yuichiro, Matsui, Takaaki, Yoshitane, Hikari, Fukada, Yoshitaka, Shinohara, Kazuyuki, and Bessho, Yasumasa

Abstract

The circadian clock possesses robust systems to maintain the rhythm approximately 24 h, from cellular to organismal levels, whereas aging is known to be one of the risk factors linked to the alternation of circadian physiology and behavior. The amount of many metabolites in the cells/body is altered with the aging process, and the most prominent metabolite among them is the oxidized form of nicotinamide adenine dinucleotide (NAD+), which is associated with posttranslational modifications of acetylation and poly-ADP-ribosylation status of circadian clock proteins and decreases with aging. However, how low NAD+ condition in cells, which mimics aged or pathophysiological conditions, affects the circadian clock is largely unknown. Here, we show that low NAD+ in cultured cells promotes PER2 to be retained in the cytoplasm through the NAD+/SIRT1 axis, which leads to the attenuated amplitude of Bmal1 promoter-driven luciferase oscillation. We found that, among the core clock proteins, PER2 is mainly affected in its subcellular localization by NAD+ amount, and a higher cytoplasmic PER2 localization was observed under low NAD+ condition. We further found that NAD+-dependent deacetylase SIRT1 is the regulator of PER2 subcellular localization. Thus, we anticipate that the altered PER2 subcellular localization by low NAD+ is one of the complex changes that occurs in the aged circadian clock., Frontiers in Neuroscience, 15, art. no. 647589; 2021

Published
2021

14. Mechanisms of cadmium-induced chronotoxicity in mice

Author

Miura, Nobuhiko, primary, Ashimori, Atsushige, additional, Takeuchi, Asuka, additional, Ohtani, Katsumi, additional, Takada, Naoko, additional, Yanagiba, Yukie, additional, Mita, Masaharu, additional, Togawa, Masako, additional, and Hasegawa, Tatsuya, additional

Published
2013
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