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Muhammad Atif,1 Saima Asghar,1 Irem Mushtaq,2 Iram Malik,1 Anum Amin,1 Zaheer-Ud-Din Babar,3 Shane Scahill4 1Department of Pharmacy, The Islamia University of Bahawalpur, Bahawalpur, Pakistan; 2Department of Education, The Islamia University of Bahawalpur, Bahawalpur, Pakistan; 3Department of Pharmacy, University of Huddersfield, Huddersfield, UK; 4School of Management, Massey Business School, Massey University, Auckland, New Zealand Purpose: This study investigates the knowledge, attitudes and practices of the general public regarding the use of antibiotics in community pharmacy, in Pakistan.Methods: This is a mixed method study where data were collected through a validated questionnaire and semi-structured interviews. Convenience sampling techniques were used to recruit participants from the general public of Bahawalpur, Pakistan who visited pharmacies to purchase antibiotics between 1 June 2018 and 31 July 2018. Descriptive statistics and regression analyses were used to tabulate the results of quantitative data while inductive thematic analysis was used to identify themes and draw conclusions from the qualitative data.Results: Over 60% of the 400 survey participants (n=246; 61.5%) had a moderate level of antibiotic knowledge; however, attitudes regarding antibiotics use were poor in half the sample (n=201; 50.3%). More than half (n=226; 56.6%) of the respondents stated that antibiotics could cure all types of infections. Just under one third (n=129; 32.3%) of respondents obtained the appropriate dosage regimen while the majority did not complete the course (n=369; 92.3%); stopping when they felt better. Inductive thematic analysis yielded four themes, 10 subthemes and 27 categories. Two subthemes were related to knowledge, one to attitude, three to practices and four subthemes were related to suggestions to improve the healthcare system. Inappropriate antibiotic practices included: lack of consultation with healthcare professionals, purchase of antibiotics witho