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In this investigation, single nucleotide variants (SNVs) within the chicken interferon-inducible transmembrane protein (chIFITM) genes were explored in Aseel and Kadaknath breeds. Comparative analysis with the GRCg6a reference genome revealed 9 and 16 SNVs in the chIFITM locus for Aseel and Kadaknath breeds, respectively. When referencing the Genome Reference Consortium GRCg7b, Kadaknath exhibited 10 variants, contrasting with none in Aseel. Notably, 17, 8, 2, and 5 SNVs were identified in chIFITM1, chIFITM2, chIFITM3, and chIFITM5 genes, with chIFITM1 showing the highest polymorphism in Kadaknath, featuring 10 intronic variants, including three SNVs (rs16457112, rs16457111, and rs313341707) common to both breeds. Two synonymous exonic variants (g.1817767C > A and g.1819102C > T) were also noted in chIFITM1. Although chIFITM protein sequences were generally conserved, genetic variations clustered predominantly in UTR and intronic regions. Examination of immune response dynamics in live embryos uncovered notable variations in chIFITM gene expression across diverse organs and chicken breeds. Specifically, chIFITM1 mRNA was abundant in cecal tonsils for both breeds and bursa of Aseel (7.61 folds), but it was absent in the heart and lung tissues of both breeds. Conversely, chIFITM3 consistently exhibited heightened expression, particularly in bursa of Aseel (10.23 folds). Whereas mRNA of the chIFITM2 gene was found to be abundant in the heart of Kadaknath (11.03 folds) and lung of both breeds. Furthermore, the expression pattern of chIFITM5 diverged between the two breeds, the heart of Kadaknath chickens showed highest (10.45 folds). The study discovered that breed-specific genetic variants within these genes present a potential pathway for selection and breeding to improve disease resistance in chicken. The observed genetic variation among chicken populations highlights the critical importance of these variants in reinforcing virus resistance, exhibiting applicability across a wide range of breeds.

This study evaluated the effects of various bedding materials on hemato-biochemical parameters in Aseel and Kadaknath chickens. Sixty day-old birds of each breed were raised on four different bedding materials for 16 weeks, with blood samples collected at the the end of trial for haemto-biochemical analysis. Sand bedding notably increased hemoglobin and packed cell volume in Aseel chickens, while sawdust and wheat straw influenced serum glucose, total serum protein, and serum albumin levels. For Kadaknath chickens, sand and sawdust bedding impacted serum albumin levels, while wheat straw affected serum glucose levels. Despite breed-specific differences, serum total protein, albumin, alanine transaminase, aspartate transaminase, and glucose levels remained consistent across bedding material groups. However, Aseel chickens consistently exhibited lower serum creatinine levels compared to Kadaknath chickens. These findings underscore the adaptability of poultry to varying bedding environments. Further research is needed to understand the underlying mechanisms and optimize bedding material selection for enhanced poultry health and productivity. [ABSTRACT FROM AUTHOR]

The present study was conducted to compare the microscopic and macroscopic parameters of semen of three Indian indigenous chicken breeds viz. Aseel, Kadaknath and native chicken of Kerala. The semen volume in native chicken of Kerala was significantly higher compared to that of Aseel and Kadaknath roosters. Significantly (p

The study compares the nutritional and sensory properties of Aseel and Broiler chicken meat, highlighting their high protein content and essential bioactive compounds. This study provides a detailed comparison of the quality and sensory properties of Aseel and Broiler chicken meat, contributing valuable insights into poultry meat production and consumption. Results indicated that Aseel chicken exhibited higher nutritional value, with elevated levels of minerals and protein (17.95%) and lower fat content (1.53%) than Broiler chicken, which had 15.46% protein and 1.95% fat. Additionally, Aseel chicken has lower Ash, moisture, water-holding capacity, and pH, indicating better oxidative stability. Its meat is darker, yellower, and redder with lower L* and higher a* and b* values. Aseel chicken muscles contain fatty acids like capric acid, pentacyclic, heptadecenoic acid, stearic acid, and arachidic acid, while broiler chicken has tricyclic acid, myristic acid, and palmitoleic acid. Aseel chicken scores higher for aroma, flavor, taste, and appearance, while broiler chicken excels in tenderness. This study underscores the superior nutritional and sensory attributes of Aseel chicken, promoting its potential in poultry meat production. [ABSTRACT FROM AUTHOR]

In this study, comparative analysis of skeletal muscle transcriptome was carried out for four biological replicates of Aseel, a fighter type breed and Punjab Brown, a meat type breed of India. The profusely expressed genes in both breeds were related to muscle contraction and motor activity. Differential expression analysis identified 961 up-regulated and 979 down-regulated genes in Aseel at a threshold of log2 fold change ≥ ±2.0 (padj<0.05). Significantly enriched KEGG pathways in Aseel included metabolic pathways and oxidative phosphorylation, with higher expression of genes associated with fatty acid beta-oxidation, formation of ATP by chemiosmotic coupling, response to oxidative stress, and muscle contraction. The highly connected hub genes identified through gene network analysis in the Aseel gamecocks were HNF4A, APOA2, APOB, APOC3, AMBP, and ACOT13, which are primarily associated with energy generating metabolic pathways. The up-regulated genes in Punjab Brown chicken were found to be related to muscle growth and differentiation. There was enrichment of pathways such as focal adhesion, insulin signaling pathway and ECM receptor interaction in these birds. The results presented in this study help to improve our understanding of the molecular mechanisms associated with fighting ability and muscle growth in Aseel and Punjab Brown chicken, respectively. [ABSTRACT FROM AUTHOR]

Simple Summary: Aseel and Kadaknath are indigenous chicken breeds in India. Due to their distinctiveness, these two breeds are becoming more important. Aseel has a reputation for cockfighting and having high-quality meat. The black flesh of Kadaknath is famous, and it is mostly raised for its meat and eggs. Both breeds have heat and disease tolerances. The chicken interferon-inducible transmembrane protein (chIFITM) genes function by preventing the entry of viruses into host cells, inhibiting viral replication, and controlling the viral load. This research was conducted to ascertain the level of chIFITM gene expression against the Newcastle disease virus (NDV) in chicken embryo fibroblast cells of both breeds. There are five members of the chIFITM gene family: chIFITM1, chIFITM2, chIFITM3, chIFITM5, and chIFITM10. chIFITM1, 2, and 3 have immune-related activity; as a result, these three are referred to as immune-related IFITM (IR-IFITM). All the chIFITM genes were shown to be highly expressed in the CEF cells of both breeds during our investigation. The Kadaknath CEF cells had a significantly low viral load and a high quantity of mRNAs for the chIFITM genes when the breeds were compared. Aseel cells demonstrated an earlier onset of NDV-induced cytopathic changes compared to Kadaknath. Newcastle disease (ND) is highly contagious and usually causes severe illness that affects Aves all over the world, including domestic poultry. Depending on the virus's virulence, it can impact the nervous, respiratory, and digestive systems and cause up to 100% mortality. The chIFITM genes are activated in response to viral infection. The current study was conducted to quantify the mRNA of chIFITM genes in vitro in response to ND viral infection. It also examined its ability to inhibit ND virus replication in chicken embryo fibroblast (CEF) cells of the Aseel and Kadaknath breeds. Results from the study showed that the expression of all chIFITM genes was significantly upregulated throughout the period in the infected CEF cells of both breeds compared to uninfected CEF cells. In CEF cells of the Kadaknath breed, elevated levels of expression of the chIFITM3 gene dramatically reduced ND viral growth, and the viral load was 60% lower than in CEF cells of the Aseel breed. The expression level of the chIFITMs in Kadaknath ranged from 2.39 to 11.68 log2 folds higher than that of control CEFs and was consistently (p < 0.01) higher than Aseel CEFs. Similar to this, theIFN-γ gene expresses strongly quickly and peaks at 13.9 log2 fold at 48 hpi. Based on these cellular experiments, the Kadaknath breed exhibits the potential for greater disease tolerance than Aseel. However, to gain a comprehensive understanding of disease resistance mechanisms in chickens, further research involving in vivo investigations is crucial. [ABSTRACT FROM AUTHOR]

The present study was undertaken to characterize the ultrastructural morphology of the blood cells of commonly reared chickens in the state of Mizoram, India under backyard poultry farming. For this study, 2 ml of whole blood was aseptically collected from the wings veins of 12 chickens of three different breeds namely the Zoar, Aseel and Rhode Island Red and processed for ultrastructural imaging under standard protocols. Under scanning electron microscopy (SEM) the matured erythrocytes of Zoar, Aseel and Rhode Island Red appeared elliptical in shape while the leukocytes and thrombocytes appeared round in shape with variable surface modifications. Under transmission electron microscopy (TEM) the granules of the heterophils of Zoar, Aseel and Rhode Island Red appeared predominantly fusiform in shape, the granules of the eosinophils appeared round in shape and that of the basophils appeared pleomorphic in shape. The cytoplasm of the monocytes, medium‐to‐large lymphocytes and thrombocytes of Zoar, Aseel and Rhode Island Red under TEM appeared to be vacuolated and granular while that of the small lymphocytes appeared to be granular but non‐vacuolated. The study concluded that the ultrastructural characteristics of the blood cells of the three breeds of chicken studied were almost similar to the blood cells of other birds reported earlier. [ABSTRACT FROM AUTHOR]

Aseel, a popular breed of native chicken, characterized by its pugnacity, fighting strength and royal gait, is being used to create crosses for domestic chicken production. However, information on its growth models is scanty. An experiment was conducted to evaluate different non-linear models and to find out best fitting model in Aseel, being maintained at Central Avian Research Institute, Izatnagar, Bareilly. Data on body weights from 12-weeks of age to 20-weeks of age at biweekly intervals were recorded on a random bred single-hatched flock. Owing to the non-linear characteristic of growth, three non-linear models namely, Gompertz, Bertalanffy and Logistic models were evaluated. Goodness of fit for all the models were checked using coefficient of determination (R²), adjusted coefficient of determination (Adj-R²), mean square error (MSE), mean absolute error (MAE) and Akaike information criterion (AIC). The Bertalanffy model most accurately characterized the growth trend in males, females and pooled sex data. The study revealed that this model may be used to ascertain the average body weights in Aseel chicken under random mating. The investigation has generated baseline data on growth modelling of random bred groups and may be used in similar investigations on other native chicken breeds. [ABSTRACT FROM AUTHOR]

In this investigation, single nucleotide variants (SNVs) within the chicken interferon-inducible transmembrane protein ( chIFITM ) genes were explored in Aseel and Kadaknath breeds. Comparative analysis with the GRCg6a reference genome revealed 9 and 16 SNVs in the chIFITM locus for Aseel and Kadaknath breeds, respectively. When referencing the Genome Reference Consortium GRCg7b, Kadaknath exhibited 10 variants, contrasting with none in Aseel. Notably, 17, 8, 2, and 5 SNVs were identified in chIFITM1 , chIFITM2 , chIFITM3 , and chIFITM5 genes, with chIFITM1 showing the highest polymorphism in Kadaknath, featuring 10 intronic variants, including three SNVs (rs16457112, rs16457111, and rs313341707) common to both breeds. Two synonymous exonic variants (g.1817767C > A and g.1819102C > T) were also noted in chIFITM1. Although chIFITM protein sequences were generally conserved, genetic variations clustered predominantly in UTR and intronic regions. Examination of immune response dynamics in live embryos uncovered notable variations in chIFITM gene expression across diverse organs and chicken breeds. Specifically, chIFITM1 mRNA was abundant in cecal tonsils for both breeds and bursa of Aseel (7.61 folds), but it was absent in the heart and lung tissues of both breeds. Conversely, chIFITM3 consistently exhibited heightened expression, particularly in bursa of Aseel (10.23 folds). Whereas mRNA of the chIFITM2 gene was found to be abundant in the heart of Kadaknath (11.03 folds) and lung of both breeds. Furthermore, the expression pattern of chIFITM5 diverged between the two breeds, the heart of Kadaknath chickens showed highest (10.45 folds). The study discovered that breed-specific genetic variants within these genes present a potential pathway for selection and breeding to improve disease resistance in chicken. The observed genetic variation among chicken populations highlights the critical importance of these variants in reinforcing virus resistance, exhibiting applicability across a wide range of breeds., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2024 The Authors.)

Disease investigation was made in a native chicken farm with 150 numbers of 12 weeks old Aseel chickens kept in Iruvelpattu village, Villupuram district of Tamil Nadu. Clinical signs like dullness, inappetance, brownish diarrhoea in few birds and mortality of 5 birds within a span of 3 days were reported (3.33%). Necropsy examination of dead birds revealed that emaciated carcass, soiling of vent with reddish droppings, swelling of small intestine with haemorrhages and also duodenum with cylindrical worms. Examination of small intestinal contents and mucosal scrapings confirmed numerous unsporulated oocysts of Eimeria sp. and eggs of Ascaridia galli. The adult roundworms were identified as Ascaridia galli. Examination of droppings from live birds also confirmed of Eimeria (23.33%) and Ascaridia galli eggs (26.66%). Based on the lesions produced in mid-small intestine, oocysts morphometry and sporulation time involved, it was identified as Eimeria necatrix. The present study reports for concurrent parasitic infections of Eimeria necatrix and Ascaridia galli and importance of specific treatment and deworming for endoparasites in native chickens.

Newcastle disease (ND) is highly contagious and usually causes severe illness that affects Aves all over the world, including domestic poultry. Depending on the virus’s virulence, it can impact the nervous, respiratory, and digestive systems and cause up to 100% mortality. The chIFITM genes are activated in response to viral infection. The current study was conducted to quantify the mRNA of chIFITM genes in vitro in response to ND viral infection. It also examined its ability to inhibit ND virus replication in chicken embryo fibroblast (CEF) cells of the Aseel and Kadaknath breeds. Results from the study showed that the expression of all chIFITM genes was significantly upregulated throughout the period in the infected CEF cells of both breeds compared to uninfected CEF cells. In CEF cells of the Kadaknath breed, elevated levels of expression of the chIFITM3 gene dramatically reduced ND viral growth, and the viral load was 60% lower than in CEF cells of the Aseel breed. The expression level of the chIFITMs in Kadaknath ranged from 2.39 to 11.68 log2 folds higher than that of control CEFs and was consistently (p < 0.01) higher than Aseel CEFs. Similar to this, theIFN-γ gene expresses strongly quickly and peaks at 13.9 log2 fold at 48 hpi. Based on these cellular experiments, the Kadaknath breed exhibits the potential for greater disease tolerance than Aseel. However, to gain a comprehensive understanding of disease resistance mechanisms in chickens, further research involving in vivo investigations is crucial.

Based on the history and clinical signs, a 1-year-old Aseel breed domestic fowl reared in backyard farming was diagnosed with crop impaction. An ingluviotomy was performed, and the impacted grass and plastic materials were removed. This case was followed up for 2 months and no complications were reported. The surgery resulted in a favorable prognosis and the case is discussed here. [ABSTRACT FROM AUTHOR]

The present study was conducted to assess and compare the performance of Aseel and Kadaknath chicken in addition to the estimation of genetic and phenotypic parameters of growth, production and reproduction traits in both indigenous breeds. Single-generation data (2018) were collected from the records maintained at Poultry Breeding Farm, Department of Animal Genetics and Breeding, LUVAS, Hisar. The least-squares means for different growth, reproduction and production traits, viz., body weights at 8 (BW8), 12 (BW12), 16 (BW16), 20 (BW20) and 40 (BW40) weeks of age, age at first egg (AFE), egg production up to 40 weeks of age (EP40) and egg weight at 40 weeks of age (EW40), were 461.41 ± 10.09 g, 683.64 ± 15.10 g, 886.01 ± 20.24 g, 1117.30 ± 22.68 g, 1772.02 ± 18.86 g, 182.66 ± 1.98 days, 64.89 ± 2.10 and 47.23 ± 0.42 g in Aseel breed. The corresponding figures in Kadaknath breed were 343.63 ± 5.67 g, 552.35 ± 6.97 g, 719.04 ± 9.02 g, 920.47 ± 11.20 g, 1581.13 ± 13.37 g, 169.83 ± 0.95 days, 55.48 ± 1.22 and 41.99 ± 0.29 g, respectively. Higher body weights were observed for Aseel breed compared to Kadaknath breed at different ages. The effect of the hatch was significant for all the growth traits and AFE in Kadaknath chicken, while it was significant only for BW12 in Aseel chicken. Genetic correlations among body weights at different ages and AFE in both the breeds were found to be negative, indicating that lower body weights of birds within a breed lead to delay in AFE. Heritability estimates for different performance traits ranged from moderate to high (0.26–0.70) in Aseel and low to moderate (0.12–0.37) in Kadaknath chicken, suggesting the scope of improvement through individual and family selection in both the indigenous breeds. [ABSTRACT FROM AUTHOR]

The data on 837 birds representing five crosses (Aseel x PD-1, Aseel x PD-2, Aseel x PD-6, Aseel x PB-1 and Aseel x PB-2) survived up to 12 weeks of age were evaluated for various growth, meat quality, meat composition, sensory attributes and carcass traits as an alternative to native chicken farming. Body weights and shank lengths significantly (P≤0.05) differed at all ages. Aseel crosses with coloured broiler lines (PB-1 and PB-2) had significantly (P≤0.05) higher body weights while Aseel x PD-1 cross recorded higher shank length at all ages. The fat and ash proportion varied significantly (P≤0.05) among the crosses. The pH values of the breast meat ranged 5.89 to 6.29. The Shear force values (SFV) and Hydroxyproline (HP) concentration differed significantly (P≤0.05) among the crosses. Meat colour differed significantly (P≤0.05) with respect to yellowness (b*) and red (a*) colouration. The proportion of legs, wings, back, neck, meat and bone significantly (P≤0.05) differed among the crosses. The cluster analysis of means of all traits revealed that least distance exists between Aseel x PD-1 and Aseel x PD-6. The study concludes that, Aseel x PD-1 and Aseel x PD-6 crosses may be a viable and suitable alternative for native chicken farming.

ABSTRACT: Egg quality parameters are very crucial for the egg industry as egg configuration affects grading, price, hatchability, chick weight, and consumer preference. Current study was undertaken to assess and compare the egg quality parameters in 2 important Indian breeds Aseel and Kadaknath reared under backyard system. Different parameters of external and internal egg quality were measured. Biochemical parameters of egg yolk estimated were egg yolk cholesterol, high density lipoprotein (HDL), low density lipoprotein (LDL), and triacylglycerol. Means for external characters of Aseel eggs viz., egg weight, egg length, egg width, shape index, shell weight, shell thickness, and shell ratio were 41.7 g, 5.16 cm, 3.85 cm, 74.75%, 4.65 g, 0.35 mm, and 11.23%, and corresponding values for Kadaknath eggs were 40.59 g, 5.13 cm, 3.79 cm, 74.02%, 4.28 g, 0.34 mm, and 10.61%, respectively. Average albumin length, width, height, and albumin index were 83.73 mm, 65.4 mm, 6.02 mm, and 9.26% for Aseel eggs and 82.27 mm, 64.80 mm, 5.52 mm and 8.52% for Kadaknath eggs, respectively. Mean values for yolk length, width, height and yolk index were 43.32 mm, 40.10 mm, 15.30 mm, and 38.30% in Aseel and 42.15 mm, 38.97 mm, 14.26 mm and 36.66% in Kadaknath breed, respectively. Mean values for egg yolk cholesterol, HDL, LDL, and triacylglycerol were estimated as 9.38, 1.74, 0.17, and 1.94 mg/g in Aseel eggs and 8.38, 1.84, 0.11, and 1.76 mg/g in Kadaknath eggs, respectively. Biochemical analysis revealed that egg yolk quality of Kadaknath eggs was comparatively better than Aseel eggs.

The study was conducted to evaluate the effect of thermal conditioning on serum levels of Triiodothyronine (T3), thyroxine (T4) and corticosterone (CORT) in four different chicken strains namely Aseel, Naked Neck, Assel × Nandanam chicken-4 and Naked Neck × Nandanam Broiler-3. Chicks were divided into control (C; ambient temperature 28±1°C) and heat exposed groups (H; 39±1°C for 2 hours; 0-2 and 5-6 weeks of age). At 12th week, control group were divided into two groups, i.e. unexposed control (C) and exposed control (CE). Birds from H group (HE) and CE group were thermally challenged at 39±1°C for 4 hours daily on 12th week. On 42nd and 84th day, blood was withdrawn from each group, serum separated and processed for T3, T4 and CORT. The results indicated that the thermal conditioning had significantly decreased T3, T4 and increased CORT levels, irrespective of strains. At thermal challenge, a drastic drop in CORT level and improvement in thyroid hormone levels were noticed in the preconditioned birds. From present study, it was concluded that, the birds are able to withstand the heat stress effect which was indicated by drop in CORT level.

ABSTRACT The present study aimed at evaluating the effects of dietary selenium (Se) supplementation on egg morphometric and quality traits in Aseel chicken. In total, 84 adult molted Aseel hens, comprising 4 varieties (Lakha, Mushki, Peshawari, and Mianwali), were divided into 3 dietary treatments (organic at 0.30 ppm, inorganic at 0.30 ppm, and without selenium) with 7 replicates (1 bird in each) under randomized complete block design. Data were analyzed using factorial ANOVA and means were compared using Duncan’s Multiple Range Test. The results indicated greater egg length in Mushki and Mianwali, lower egg breadth in Lakha, the highest (p≤0.05) egg volume and surface area in Mianwali, and higher shape index in Lakha and Peshawari. Lakha had the highest Haugh unit and lower eggshell thickness with and without membrane than the other three varieties. Interaction of Se sources and Aseel varieties exhibited the highest (p≤0.05) egg volume and surface area in Mianwali variety when fed on organic Se supplemented diet. Similarly, the interaction showed the highest eggshell thickness with membrane in Peshawari and without membrane in Mianwali when fed on inorganic Se supplemented diet. From the findings, it can be concluded that egg physical characteristics of Mianwali variety hens can be improved by the supplementation of Se-enriched yeast, as an organic Se source, in the diet.

Disease investigation was made in a native chicken farm with 150 numbers of 12 weeks old Aseel chickens kept in Iruvelpattu village, Villupuram district of Tamil Nadu. Clinical signs like dullness, inappetance, brownish diarrhoea in few birds and mortality of 5 birds within a span of 3 days were reported (3.33%). Necropsy examination of dead birds revealed that emaciated carcass, soiling of vent with reddish droppings, swelling of small intestine with haemorrhages and also duodenum with cylindrical worms. Examination of small intestinal contents and mucosal scrapings confirmed numerous unsporulated oocysts of Eimeria sp. and eggs of Ascaridia galli. The adult roundworms were identified as Ascaridia galli. Examination of droppings from live birds also confirmed of Eimeria (23.33%) and Ascaridia galli eggs (26.66%). Based on the lesions produced in mid-small intestine, oocysts morphometry and sporulation time involved, it was identified as Eimeria necatrix. The present study reports for concurrent parasitic infections of Eimeria necatrix and Ascaridia galli and importance of specific treatment and deworming for endoparasites in native chickens. [ABSTRACT FROM AUTHOR]

The data on 837 birds representing five crosses (Aseel x PD-1, Aseel x PD-2, Aseel x PD-6, Aseel x PB-1 and Aseel x PB-2) survived up to 12 weeks of age were evaluated for various growth, meat quality, meat composition, sensory attributes and carcass traits as an alternative to native chicken farming. Body weights and shank lengths significantly (P≤0.05) differed at all ages. Aseel crosses with coloured broiler lines (PB-1 and PB-2) had significantly (P≤0.05) higher body weights while Aseel x PD-1 cross recorded higher shank length at all ages. The fat and ash proportion varied significantly (P≤0.05) among the crosses. The pH values of the breast meat ranged 5.89 to 6.29. The Shear force values (SFV) and Hydroxyproline (HP) concentration differed significantly (P≤0.05) among the crosses. Meat colour differed significantly (P≤0.05) with respect to yellowness (b*) and red (a*) colouration. The proportion of legs, wings, back, neck, meat and bone significantly (P≤0.05) differed among the crosses. The cluster analysis of means of all traits revealed that least distance exists between Aseel x PD-1 and Aseel x PD-6. The study concludes that, Aseel x PD-1 and Aseel x PD-6 crosses may be a viable and suitable alternative for native chicken farming. [ABSTRACT FROM AUTHOR]

Background and Aim: Newcastle disease (ND) is considered one of the most important poultry diseases with chicken morbidity and mortality rates up to 100%. Current vaccination programs allow the use of live attenuated vaccines in the field to protect against the disease, which alone is inefficient and requires repeat booster doses. Toll-like receptor agonists (e.g., lipopolysaccharide [LPS]) as adjuvants are the ones, most extensively studied and have shown to be very promising in delivering a robust balanced immune response. In the present study, we have evaluated the potential of LPS to elicit a strong immune response with respect to the elicitation of both Th1 (cell-mediated) and Th2 (humoral) immune arms. Materials and Methods: A total of 72 apparently healthy 1-day-old indigenous unvaccinated chicks were randomly divided into six experimental Groups A to F (n=12). At 8-week of age chicks in Group A, C, and E were vaccinated with live attenuated La Sota strain ND vaccine along with LPS, bovine serum albumin, and normal saline solution, respectively, and those in Group B, D, and E were kept separately without vaccination. Sampling was done on days 0, 1, 3, 7, 14, 21, 35, and 60 after vaccination. After vaccination and respective adjuvant application, Th1 and Th2 cytokine expression were measured in mRNA of both blood and tissue samples. Results: The results were validated by, hemagglutination inhibition and enzyme-linked immunosorbent assay tests, to check for the humoral as well as cell-mediated immune response in blood serum levels. The results showed an increase in mRNA expression of the Th1 biased cytokines in Group A (LPS+NDV) as compared to the control groups. Similar mRNA expression pattern was seen in blood as well as tissue samples. Validation of results also indicates an increase in Cell-mediated Immunity as well as a humoral immune response in Group A (LPS+NDV). Conclusion: The results of the study provided enough evidence to consider LPS as a potential vaccine adjuvants candidate against ND in chicken.

Aseel is amongst the most vital date variety of Pakistan. Beside its nutritional value, it also got remedial uses therefore for the first time different in-vitro bioassays were performed to assess its medicinal value. Aqueous (DFAE) and ethanol (DFEE) extracts of fresh Aseel dates were used for the purpose. Microplate alamar blue assay was done for antibacterial activity, Brine shrimp lethality test for cytotoxicity and MTT assays with different cancer cell lines were used for anti-cancer activity. Antioxidant and anti-inflammatory activity were also evaluated by free radical scavenging bioassay and chemiluminescence technique. Alamar blue assay of both extracts exhibited weak antibacterial activity against E.coli, S, flexenari and S. aureus. Brine shrimp lethality revealed absence of cytotoxicity at 1000µg/mL concentration. DFEE 50 µg/mL was effective against MCF-7,MDA-MB-231, PC3, 3T3 and Hela cancer cell lines showing 17.59%, 20.90%, 37.60%, 22.35% and 36.70% inhibition whereas DFAE exhibits 20.46%, 30.86%, 15.21%, 29.70% and 16.40% inhibition respectively. Similarly both extracts also showed varying degree of anti-oxidant and antiinflammatory activity against standard drug. The results are suggestive of weak bioactivity of Aseel date extracts might because of reduced potency however further studies are required for better understanding of observed results and separation of active ingredients from Aseel dates. [ABSTRACT FROM AUTHOR]

ABSTRACT Present study evaluated carcass and meat quality attributes of broilers, broiler breeders and Aseel chickens. Sixty birds from each chicken genotype (broiler, broiler breeder and Aseel chickens; five-wk-old broilers and 60-wk-old Aseel chickens and broiler breeders) were evaluated. Birds were maintained under similar environment for 21 days, and then slaughtered to analyze their carcass qualitative and organoleptic characteristics. No carcass yield differences were detected among genotypes. Aseel chickens had heavier neck, followed by broiler breeder and broiler (p

Backyard poultry production plays a vital role in providing animal protein at affordable and sustainable manner. Growth and production potential of indigenous chicken need to be improved through selective breeding to enhance the productivity of backyard farming. PD-4 birds (Aseel, indigenous breed) were subjected to selection for higher body weight over eight generations. Study investigated effect of selection on the performance of PD-4 birds with respect to economic traits. There was significant improvement in body weight and shank length at 8 weeks of age. Egg production up to 40 weeks had improved by 19.1 eggs. Egg weight at 28, 32, 36 and 40 weeks of age improved by 0.8, 1.3, 1.7 and 1.3 g respectively. Age at sexual maturity had declined by 14.2 days. There was no significant change in fertility and hatchability traits. Survivors’ egg production up to 72 weeks of age was 156.5±2.79 eggs while hen day and hen housed egg production were 144.9 and 137.5 eggs respectively. Egg weight consistently improved from 35.13 g at age at first egg to 48.07 g at 40 weeks and to 49.63 g at 72 weeks of age. The study demonstrated that indigenous chickens could be improved for body weight and egg production without affecting other economic traits and improved PD-4 variety has the potential to be used as improved dual purpose indigenous chicken variety for low input backyard poultry farming.

Gut microbiota plays an important role in the health and performance of the host. Characterizations of gut microbiota, core microbiomes, and microbial networks in different chicken breeds are expected to provide clues for pathogen exclusion, improving performance or feed efficiency. Here, we characterized the gut microbiota of “finishing” chickens (at the end of production life) of indigenous Indian Nicobari, Ghagus, and Aseel breeds, originating from the Nicobari island, coastal India, and the Indian mainland, respectively, as well as a global commercial broiler line, VenCobb 400, using 16S rDNA amplicon sequencing. We found that diversity, as well as richness of microbiota, was higher in indigenous breeds than in the broiler line. Beta diversity analysis indicated the highest overlap between Ghagus and Nicobari breeds and a very low overlap between the broiler line and all indigenous breeds. Linear discriminant analysis effect size (LEfSe) revealed 82 breed- or line-specific phylotype operational taxonomic unit (OTU) level biomarkers. We confirm the presence of breed specific and across-breed core microbiomes. Additionally, we show the existence of breed specific complex microbial networks in all groups. This study provides the first (and comprehensive) insight into the gut microbiota of three indigenous breeds and one commercial broiler line of chickens reared without antimicrobials, and underscores the need to study microbial diversity in other indigenous breeds.

The present study was planned to explore the effects of different selenium-supplemented diets on production performance in 4 varieties of Aseel chicken, including Lakha (LK), Mushki (MS), Peshawari (PW), and Mianwali (MW). The selenium (Se) from Se-enriched yeast (SY) and sodium selenite (SS) was supplemented into a corn-soybean meal basal diet at 0.3 mg/kg, whereas control diet had no supplemental Se. In total, 84 adult molted Aseel hens (50 wk old), 21 from each variety, were randomly assigned to 12 treatment groups in a 3 × 4 factorial arrangement of 3 Se diets (organic, inorganic, and control) and 4 Aseel varieties (LK, MS, PW, and MW), under a randomized complete block design (RCBD). Each treatment had 7 replicates with individual hens per replicate. Feed intake (FI), body weight (BW), egg production (EP), egg weight (EW), egg mass (EM), FCR/dozen eggs, and FCR/kg egg mass parameters were evaluated. The results indicated that the hens fed organic Se-supplemented diet showed greater (P < 0.05) EP and EM, and improved (P < 0.05) FCR/dozen eggs and FCR/kg egg mass compared with those fed inorganic and without Se. Among varieties, MW hens showed greater (P < 0.05) BW and produced heavier (P < 0.05) eggs, whereas PW hens showed improved (P < 0.05) FCR/dozen eggs compared with those of other Aseel varieties. In conclusion, dietary supplementation of Se-enriched yeast, as an organic Se source, could be used to ameliorate the poor production performance of Aseel chicken. [ABSTRACT FROM AUTHOR]

The biological experiment was designed to study the effect of feeding ghee residue in native chicken (Aseel) for a period of 12 weeks on various parameters, viz. bi-weekly body weight, gain in body weight, feed consumption, feed conversion ratio, livability and the cost effectiveness of feeding ghee residue. A total of 300 day-old, unsexed, Aseel native chicken belonging to single hatch were utilized for this study. The treatment groups were fed with diets containing ghee residue at different inclusion levels, i.e. 0% (T1-control), 5% (T2), 10% (T3) and 15% (T4). Biological experiment revealed that there was significant difference in body weight and body weight gain and feed conversion ratio and no significant difference was observed in feed consumption and livability. The cost effectiveness of the native chicken fed with various levels of ghee residue showed increased net profit per kg live weight in the group fed 5% ghee residue. [ABSTRACT FROM AUTHOR]

Background and Aim: Newcastle disease (ND) is considered one of the most important poultry diseases with chicken morbidity and mortality rates up to 100%. Current vaccination programs allow the use of live attenuated vaccines in the field to protect against the disease, which alone is inefficient and requires repeat booster doses. Toll-like receptor agonists (e.g., lipopolysaccharide [LPS]) as adjuvants are the ones, most extensively studied and have shown to be very promising in delivering a robust balanced immune response. In the present study, we have evaluated the potential of LPS to elicit a strong immune response with respect to the elicitation of both Th1 (cell-mediated) and Th2 (humoral) immune arms. Materials and Methods: A total of 72 apparently healthy 1-day-old indigenous unvaccinated chicks were randomly divided into six experimental Groups A to F (n=12). At 8-week of age chicks in Group A, C, and E were vaccinated with live attenuated La Sota strain ND vaccine along with LPS, bovine serum albumin, and normal saline solution, respectively, and those in Group B, D, and E were kept separately without vaccination. Sampling was done on days 0, 1, 3, 7, 14, 21, 35, and 60 after vaccination. After vaccination and respective adjuvant application, Th1 and Th2 cytokine expression were measured in mRNA of both blood and tissue samples. Results: The results were validated by, hemagglutination inhibition and enzyme-linked immunosorbent assay tests, to check for the humoral as well as cell-mediated immune response in blood serum levels. The results showed an increase in mRNA expression of the Th1 biased cytokines in Group A (LPS+NDV) as compared to the control groups. Similar mRNA expression pattern was seen in blood as well as tissue samples. Validation of results also indicates an increase in Cell-mediated Immunity as well as a humoral immune response in Group A (LPS+NDV). Conclusion: The results of the study provided enough evidence to consider LPS as a potential vaccine adjuvants candidate against ND in chicken. [ABSTRACT FROM AUTHOR]

Aseel is an important native chicken breed of India, known for its martial qualities (aggressive fighting abilities), pugnacity, and majestic gait. The aim of the study is to conserve and characterize the Aseel germplasm, which is considered to be endangered. The birds were maintained on deep litter under a simulated backyard type of housing having night shelter and a free-range area. A total of 313 chicks produced in the second generation from the flock collected from native tract in Andhra Pradesh was characterized for morphological, growth, production, and meat quality parameters. Aseel birds were characterized by multicolored plumage (predominantly dark brown, black, golden, etc.) with solid feather patterns and normal distribution. Ear lobes were red (92%) and small in size, while 98% of the birds had red colored pea combs with variations in intensity of color. The shank color was yellow in the majority (65%) of the birds. The skin color was white (98%) with pinkish red coloration on exposed body parts, especially on the breast. The fertility and hatchability on total eggs were 67.2 and 41.4%, respectively. Cocks were heavier (P ≤ 0.05) with distinct sexual dimorphism in Aseel. The body weight of hens and cocks was 1,704.4 ± 23.2 and 2,702.5 ± 28.1 g at 40 wk and 2,333.7 ± 26.1 and 3,793.7 ± 20.8 g at 72 wk of age, respectively. The age at sexual maturity was 214.0 ± 6.0 days. The egg production up to 40, 52, and 64 wk of age was 18.0 ± 1, 30.0 ± 2.0, and 47 ± 3 eggs, respectively. The annual egg production (72 wk) was 64 ± 6 eggs. The proximate composition of breast muscle was; protein 21.5 ± 0.5%, fat 3.4 ± 0.1%, ash 2.0 ± 0.1%, and moisture 73.3 ± 0.5%. The pH of breast muscle was 6.0 ± 0.03 and the cholesterol content was 72.5 ± 6.7 mg/100 g. Efforts are on for improving the productivity in the flock without compromising the original breed characteristics. [ABSTRACT FROM AUTHOR]

A study was conducted to evaluate the effect of dietary selenium (Se) sources (organic and inorganic Se at 0.30 ppm and basal diet at 0 ppm level of supplemented Se) on hatching traits in four varieties of Aseel chicken, Lakha, Mushki, Peshawari, and Mianwali. In total, 84 adult molted hens (50 wk old), 21 from each variety, were randomly assigned to 12 treatment groups in a 3 (Se diets) x 4 (Aseel varieties) factorial arrangement under a randomized complete block design. Each treatment was replicated 7 times with individual hens in each. Settable egg, fertility, hatch of fertile eggs, hatchability, A-grade chick, and embryonic mortality parameters were evaluated. The results indicated that the birds fed an organic Se supplemented diet had greater (P < 0.05) settable eggs, fertility, hatch of fertile eggs, hatchability, and A-grade chicks and reduced embryonic mortality than those fed inorganic or no Se. Among varieties, Mushki had lower (P < 0.05) fertility, hatch of fertile eggs, hatchability, and A-grade chicks than rest of three varieties. Interaction of Se sources and varieties indicated that dietary organic Se supplementation improved (P < 0.05) hatch of fertile eggs in Peshawari and Mianwali, whereas hatchability only in Peshawari variety and reduced embryonic mortality in Mianwali. It was concluded that dietary supplementation of organic Se could be used to improve hatching traits as well as reduce embryonic mortality in native Aseel chicken. [ABSTRACT FROM AUTHOR]