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26 results on '"Arunorat, Jirapat"'

10. Comparative Efficacy of Chimeric Porcine Circovirus (PCV) Vaccines against Experimental Heterologous PCV2d Challenges

Author

Wongchanapai, Pichanun, primary, Yamsakul, Panuwat, additional, Arunorat, Jirapat, additional, Guntawang, Thunyamas, additional, Sittisak, Tidaratt, additional, Srivorakul, Saralee, additional, Photichai, Kornravee, additional, Thanawongnuwech, Roongroje, additional, Sukmak, Manakorn, additional, and Pringproa, Kidsadagon, additional

Published
2023
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12. Brazilin from Caesalpinia sappan inhibits viral infection against PRRSV via CD163ΔSRCR5 MARC-145 cells: an in silico and in vitro studies.

Author

Arjin, Chaiwat, Tateing, Suriya, Potapohn, Nuttha, Arunorat, Jirapat, Pringproa, Kidsadagon, Lumsangkul, Chompunut, Seel-audom, Mintra, Ruksiriwanich, Warintorn, and Sringarm, Korawan

Subjects
CAESALPINIA, PORCINE reproductive & respiratory syndrome, VIRUS diseases, IN vitro studies, BINDING energy, PLANT viruses
Abstract

This research aimed to identify bioactive compounds from Caesalpinia sappan extract that function as novel porcine reproductive and respiratory syndrome virus (PRRSV) infection inhibitors by computational molecular screening. We obtained a set of small-molecule compounds predicted to target the scavenger receptor cysteine-rich domain 5 (SRCR5) of CD163. In addition, the functions of positive hits were assessed and verified utilizing an in vitro antiviral activity assay with PRRSV-infected MARC-145 cells. Combining molecular docking with the results of binding affinity and ligand conformation, it was found that brazilin had the highest binding energy with the SRCR5 receptor compared to catechin and epicatechin (− 5.8, − 5.5, and − 5.1 kcal/mol, respectively). In terms of molecular mechanics, the binding free energy between the SRCR5 receptor was − 15.71 kcal/mol based on the Poisson-Boltzmann surface area of brazilin. In addition, PRRSV infection in MARC-145 cells was significantly inhibited by brazilin compared to the control (virus titer, 4.10 vs. 9.25 TCID50/mL, respectively). Moreover, brazilin successfully limited the number of PRRSV RNA copies in MARC-145 cells as determined by RT-qPCR. By inhibiting the PRRSV-CD163 interaction with brazilin from Caesalpinia sappan, it may be possible to prevent PRRSV infection in pigs, as suggested by this research. [ABSTRACT FROM AUTHOR]

Published
2022
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16. Porcine circovirus type 3 (PCV3) shedding in sow colostrum

Author

Kedkovid, Roongtham, primary, Woonwong, Yonlayong, additional, Arunorat, Jirapat, additional, Sirisereewan, Chaitawat, additional, Sangpratum, Nattaphong, additional, Kesdangsakonwut, Sawang, additional, Tummaruk, Padet, additional, Teankum, Komkrich, additional, Assavacheep, Pornchalit, additional, Jittimanee, Suphattra, additional, and Thanawongnuwech, Roongroje, additional

Published
2018
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17. Detection of PRRSV circulation using oral fluid samples for nursery management in endemically PRRSV-infected farms

Author

Woonwong, Yonlayong, primary, Kedkovid, Roongtham, additional, Arunorat, Jirapat, additional, Sirisereewan, Chaitawat, additional, Nedumpun, Teerawut, additional, Poonsuk, Korakrit, additional, Panyasing, Yaowalak, additional, Poolperm, Pariwat, additional, Boonsoongnern, Alongkot, additional, and Thanawongnuwech, Roongroje, additional

Published
2018
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18. Porcine circovirus type 3 (PCV3) infection in grower pigs from a Thai farm suffering from porcine respiratory disease complex (PRDC)

Author

Kedkovid, Roongtham, primary, Woonwong, Yonlayong, additional, Arunorat, Jirapat, additional, Sirisereewan, Chaitawat, additional, Sangpratum, Nattaphong, additional, Lumyai, Mongkol, additional, Kesdangsakonwut, Sawang, additional, Teankum, Komkrich, additional, Jittimanee, Suphattra, additional, and Thanawongnuwech, Roongroje, additional

Published
2018
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19. Oral fluid samples used for PRRSV acclimatization program and sow performance monitoring in endemic PRRS-positive farms

Author

Woonwong, Yonlayong, primary, Kedkovid, Roongtham, additional, Arunorat, Jirapat, additional, Sirisereewan, Chaitawat, additional, Nedumpun, Teerawut, additional, Poonsuk, Korakrit, additional, Panyasing, Yaowalak, additional, Poolperm, Pariwat, additional, Boonsoongnern, Alongkot, additional, and Thanawongnuwech, Roongroje, additional

Published
2017
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26. Field Efficacy of Potassium Peroxymonosulfate (PMS) Oxidizing Disinfectant (VirusnipTM) against Porcine Circovirus Type 2 (PCV2) in Gilt Acclimatization Unit.

Author

Poonsuk, Korakrit, Arunorat, Jirapat, Talummuk, Sasiwimon, Kunalintip, Rapeepat, Anuvongnukroh, Woravit, and Thanawongnuwech, Roongroje

Subjects
*DISINFECTION & disinfectants, *ACCLIMATIZATION (Plants), *VIRAL contamination, *FARM produce, *FARM management
Abstract

Virusnip™ is one of the commercial potassium peroxymonosulfate disinfectants widely used in swine farms. This disinfectant is well studied and proves to be excellent in antimicrobial activity previously. However, there is no information about its efficacy under field condition. Thus, the objective of this study was to determine the virucidal activity of Virusnip® against PCV2 in farm condition. Consequently, 1:200 concentration of VirusnipTM was sprayed for 15 minutes once or twice daily depending on the acclimatization period in gilts isolation units compared to the untreated group in the same building. Environmental swabs and oral fluid samples were collected and pooled to determine virucidal effectiveness of PCV2 in the environment as well as virus shedding in the oral secretion of the studied animals. Viral load in each group was quantified by a modified real-time PCR specific for ORF1 of PCV2. Result showed significant reduction in the viral load from the environment contamination and also from the oral fluid samples of the treatment group compared to the control group. This indicates that potassium peroxymonosulfate disinfectant (Virusnip™) can be a disinfectant of choice used for reducing the viral contamination and viral transmission among animals under field situation. [ABSTRACT FROM AUTHOR]

Published
2013

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