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19 results on '"Arun L. Jayaraman"'

1. Cannulation strategies in adult veno-arterial and veno-venous extracorporeal membrane oxygenation: Techniques, limitations, and special considerations

Author

Arun L Jayaraman, Daniel Cormican, Pranav Shah, and Harish Ramakrishna

Subjects
Acute respiratory failure, cardiogenic shock, extracorporeal membrane oxygenation, extracorporeal membrane oxygenation cannulation strategies, triple cannulation, Anesthesiology, RD78.3-87.3, Diseases of the circulatory (Cardiovascular) system, RC666-701
Abstract

Extracorporeal membrane oxygenation (ECMO) refers to specific mechanical devices used to temporarily support the failing heart and/or lung. Technological advances as well as growing collective knowledge and experience have resulted in increased ECMO use and improved outcomes. Veno-arterial (VA) ECMO is used in selected patients with various etiologies of cardiogenic shock and entails either central or peripheral cannulation. Central cannulation is frequently used in postcardiotomy cardiogenic shock and is associated with improved venous drainage and reduced concern for upper body hypoxemia as compared to peripheral cannulation. These concerns inherent to peripheral VA ECMO may be addressed through so-called triple cannulation approaches. Veno-venous (VV) ECMO is increasingly employed in selected patients with respiratory failure refractory to more conventional measures. Newer dual lumen VV ECMO cannulas may facilitate extubation and mobilization. In summary, the pathology being addressed impacts the ECMO approach that is deployed, and each ECMO implementation has distinct virtues and drawbacks. Understanding these considerations is crucial to safe and effective ECMO use.

Published
2017
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4. The Year in Cardiothoracic and Vascular Anesthesia: Selected Highlights from 2020

Author

Menachem M. Weiner, Elizabeth Zhou, Anita Malhotra, Jared W. Feinman, Matthew M. Townsley, Lourdes Al Ghofaily, Kristin Stawiarski, Sudhakar Subramani, Himani V. Bhatt, Dylan R. Addis, Lovkesh Arora, Arun L. Jayaraman, Miguel T. Teixeira, Saumil Patel, Archer Kilbourne Martin, Harish Ramakrishna, Ashley V. Fritz, Archit Sharma, and Christopher R. Cowart

Subjects
medicine.medical_specialty, Coronavirus disease 2019 (COVID-19), Heart Valve Diseases, Specialty, Transcatheter valve replacement, Left ventricular assist device, Heart transplantation, 030204 cardiovascular system & hematology, Coronary artery disease, Article, Transcatheter Aortic Valve Replacement, 03 medical and health sciences, 0302 clinical medicine, Anesthesiology, 030202 anesthesiology, Anesthesia, Cardiac Procedures, medicine, Humans, Cardiac Surgical Procedures, Cardiothoracic transplantation, Transcatheter valve repair, Heart Valve Prosthesis Implantation, SARS-CoV-2, Vascular disease, business.industry, COVID-19, Perioperative, medicine.disease, Anesthesiology and Pain Medicine, Anesthesia, Heart-Assist Devices, Cardiology and Cardiovascular Medicine, business, Vascular Surgical Procedures, Psychosocial, Theme (narrative)
Abstract

THIS SPECIAL article is the 13th in an annual series for the Journal of Cardiothoracic and Vascular Anesthesia. The authors thank the editor-in-chief, Dr Kaplan, and the editorial board for the opportunity to continue this series; namely, the research highlights of the past year in the specialty of cardiothoracic and vascular anesthesiology.1 The major themes selected for 2020 are outlined in this introduction, and each highlight is reviewed in detail in the main body of the article. The literature highlights in the specialty for 2020 begin with an update on valvular disease, with a focus on updates in management of aortic and mitral valve disorders. The second major theme is an update on coronary artery disease, with discussion of both medical and surgical management. The third major theme is focused on the perioperative management of patients with coronavirus disease 2019 (COVID-19), with the authors highlighting literature discussing medical, surgical, and anesthetic considerations for their cardiac care. The fourth major theme is an update in heart failure, with discussion of medical, psychosocial, and procedural aspects of this complicated disease process. The fifth and final theme focuses on the latest analyses regarding survival in heart transplantation. The themes selected for this 13th special article are only a few of the diverse advances in the specialty during 2020. These highlights will inform the reader of key updates on a variety of topics, leading to improvement of perioperative outcomes for patients with cardiothoracic and vascular disease.

Published
2021
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5. Extracorporeal Membrane Oxygenation in Lung Transplantation: Analysis of Techniques and Outcomes

Author

Arun L. Jayaraman, Barbara J. Wilkey, Archer Kilbourne Martin, Lavinia Kolarczyk, Harish Ramakrishna, Ashley V. Fritz, and Christoph G. Nabzdyk

Subjects
medicine.medical_specialty, business.industry, medicine.medical_treatment, Extracorporeal Membrane Oxygenation, Anesthesiology and Pain Medicine, Internal medicine, Extracorporeal membrane oxygenation, Cardiology, Humans, Medicine, Lung transplantation, Cardiology and Cardiovascular Medicine, business, Lung Transplantation, Retrospective Studies
Published
2021
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6. The Impact of Anesthetic Management on Perioperative Outcomes in Lung Transplantation

Author

Lavinia Kolarczyk, Barbara J. Wilkey, Ashley V. Fritz, Suraj M. Yalamuri, Arun L. Jayaraman, Archer Kilbourne Martin, and Harish Ramakrishna

Subjects
Lung, business.industry, medicine.medical_treatment, MEDLINE, Anesthetic management, Perioperative, Perioperative Care, Anesthesiology and Pain Medicine, medicine.anatomical_structure, Anesthesia, medicine, Humans, Lung transplantation, Cardiology and Cardiovascular Medicine, business, Anesthetics, Lung Transplantation
Published
2020
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7. The Year in Cardiothoracic and Vascular Anesthesia: Selected Highlights from 2021

Author

Jared W. Feinman, Lourdes Al Ghofaily, Saumil Patel, Selina Read, Matthew M. Townsley, Archit Sharma, Lovkesh Arora, Sudhakar Subramani, Himani V. Bhatt, Arun L. Jayaraman, Robert M. Owen, Archer Kilbourne Martin, Menachem M. Weiner, Anita Malhotra, Harish Ramakrishna, Miguel Teixeira, Ashley V. Fritz, and Elizabeth Zhou

Subjects
medicine.medical_specialty, Heart disease, Vascular disease, business.industry, SARS-CoV-2, Specialty, Aortic valve disorder, COVID-19, Disease, Perioperative, medicine.disease, Anesthesiology and Pain Medicine, Anesthesiology, Anesthesia, medicine, Humans, Cardiology and Cardiovascular Medicine, business, Theme (narrative)
Abstract

This special article is the fourteenth in an annual series for the Journal of Cardiothoracic and Vascular Anesthesia. The authors thank the Editor-in-Chief, Dr. Kaplan, and the editorial board for the opportunity to continue this series; namely, the research highlights of the past year in the specialty of cardiothoracic and vascular anesthesiology. The major themes selected for 2021 are outlined in this introduction, and each highlight is reviewed in detail in the main body of the article. The literature highlights in the specialty for 2021 begin with an update on structural heart disease, with a focus on updates in arrhythmia and aortic valve disorders. The second major theme is an update on coronary artery disease, with discussion of both medical and procedural management. The third major theme is focused on the perioperative management of patients with COVID-19, with the authors highlighting literature discussing the impact of the disease on the right ventricle and thromboembolic events. The fourth and final theme is an update in heart failure, with discussion of diverse aspects of this area. The themes selected for this fourteenth special article are only a few of the diverse advances in the specialty during 2021. These highlights will inform the reader of key updates on a variety of topics, leading to improvement of perioperative outcomes for patients with cardiothoracic and vascular disease.

Published
2021

8. TAVR Procedural Volumes and Patient Outcomes: Analysis of Recent Data

Author

Pedro Villablanca, Arun L. Jayaraman, Daniel Cormican, and Harish Ramakrishna

Subjects
medicine.medical_specialty, Transcatheter aortic, medicine.medical_treatment, Outcome analysis, 030204 cardiovascular system & hematology, Transcatheter Aortic Valve Replacement, 03 medical and health sciences, 0302 clinical medicine, Valve replacement, Risk Factors, 030202 anesthesiology, Humans, Medicine, In patient, Risk factor, business.industry, General surgery, Aortic Valve Stenosis, Perioperative, medicine.disease, Treatment Outcome, Anesthesiology and Pain Medicine, Aortic Valve, Aortic valve stenosis, Cardiology and Cardiovascular Medicine, business
Abstract

In less than 15 years, transcatheter aortic valve replacement (TAVR) has progressed from a procedure of last resort in patients at prohibitively high perioperative risk for major morbidity and mortality from surgical valve replacement to a viable alternative option to surgery in most patients with native (non-bicuspid) aortic valve stenosis. The number of medical centers offering TAVR has rapidly proliferated. There is mounting evidence that there are variations in patient outcomes associated with the yearly number of TAVR cases performed at each respective center. This review outlines the evolution of TAVR indications, common complications, the current literature addressing the association between procedural volumes and patient outcomes in TAVR, and offers a synopsis of risk factor assessment for patients considered for TAVR.

Published
2020
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9. Analysis of Outcomes in Heart Transplantation

Author

Juan G. Ripoll, Archer Kilbourne Martin, Ashley V. Fritz, Harish Ramakrishna, Robert A. Ratzlaff, Barbara J. Wilkey, and Arun L. Jayaraman

Subjects
Heart Failure, Heart transplantation, medicine.medical_specialty, business.industry, medicine.medical_treatment, medicine.disease, Cardiac Anesthesia, Anesthesiology and Pain Medicine, Heart failure, Internal medicine, Cardiology, medicine, Heart Transplantation, Humans, Cardiology and Cardiovascular Medicine, business
Published
2020
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10. Coronary Artery Bypass Grafting Versus Percutaneous Transcatheter Coronary Interventions: Analysis of Outcomes in Myocardial Revascularization

Author

Richard Sheu, Carly Peterson, Shahzad Shaefi, Harish Ramakrishna, Daniel Cormican, Arun L. Jayaraman, Seshasayee Narasimhan, and Iván J. Núñez-Gil

Subjects
Cardiac Catheterization, medicine.medical_specialty, Myocardial revascularization, Percutaneous, Bypass grafting, business.industry, Psychological intervention, Coronary Artery Disease, Percutaneous Coronary Intervention, Treatment Outcome, Anesthesiology and Pain Medicine, medicine.anatomical_structure, Internal medicine, Conventional PCI, Myocardial Revascularization, medicine, Cardiology, Humans, Coronary Artery Bypass, Cardiology and Cardiovascular Medicine, business, Artery
Published
2019
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11. Multiple myeloma and malignant lesions: a potential risk factor for local anesthetic systemic toxicity

Author

Taylor Barney, Arun L. Jayaraman, and Victoria M. Lim

Subjects
Lidocaine, Pleural effusion, medicine.drug_class, Pain, Risk Factors, Medicine, Humans, Anesthetics, Local, Bone pain, Multiple myeloma, Bupivacaine, Respiratory distress, business.industry, Local anesthetic, General Medicine, Middle Aged, medicine.disease, Anesthesiology and Pain Medicine, Anesthesia, Female, medicine.symptom, business, Cancer pain, Multiple Myeloma, medicine.drug
Abstract

BackgroundMultiple myeloma is a cancer of plasma cells that often leads to complications including osteolytic bone lesions, nephropathy and neuropathy. Multiple myeloma is only one etiology of many cancer pain conditions that may necessitate interventional pain treatment when refractory to multimodal medications. Notably, local anesthetic systemic toxicity is a rare but life-threatening complication of local anesthetic administered for these interventions.Case presentationA 50–60-year-old woman presented with multiple myeloma complicated by chronic bone pain and in an acute pain crisis. A fluoroscopic-guided L4–5 epidural catheter was placed with clinical doses of bupivacaine for comfort to undergo MRI of the spine. Soon after, she became tachycardic, tachypneic and hypoxic requiring non-invasive positive pressure airway support. As this respiratory distress was attributed to a large pleural effusion, a pigtail catheter was inserted in the intensive care unit with submaximally dosed lidocaine infiltration. She then developed a left bundle branch block followed by cardiovascular collapse minimally responsive to high-dose inotrope and vasopressor support. Lipid emulsion was started with dramatic therapeutic response and recovery to baseline. A CT of the thoracolumbar spine showed worsening extensive lytic lesions throughout all vertebral bodies and ribs from diffuse myeloma.ConclusionsPatients with oncologic lesions focal to the thoracolumbar spine may be at higher risk for local anesthetic systemic toxicity from palliative epidurals due to increased cancer-related angiogenesis. Likewise, local anesthetic infiltration for procedures near any malignant sites could have a similar risk and may require lower initial fractionated dosages with increased vigilance.

Published
2021

12. The Year in Cardiothoracic and Vascular Anesthesia: Selected Highlights from 2018

Author

Harry Garcia, Arun L. Jayaraman, Adam S. Evans, Jacob T. Gutsche, Shahzad Shaefi, Himani V. Bhatt, Bao Ha, Menachem M. Weiner, Archer Kilbourne Martin, Ron Leong, Caroline Eden, J. Ross Renew, Harish Ramakrishna, Stuart J. Weiss, Ankit Jain, Regina E. Linganna, George Silvay, Prakash A. Patel, Jared W. Feinman, John G.T. Augoustides, Mathew M. Townsley, Eric Feduska, and Ronak Shah

Subjects
Cardiac allograft, business.industry, Cardiogenic shock, Atrial fibrillation, medicine.disease, Anesthesiology and Pain Medicine, Anesthesia, Sirolimus, Practice Guidelines as Topic, medicine, Humans, Cardiac Surgical Procedures, Cardiology and Cardiovascular Medicine, business, Vascular Surgical Procedures, medicine.drug
Published
2019
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13. The Year in Cardiothoracic and Vascular Anesthesia: Selected Highlights From 2017

Author

Prakash A. Patel, Jared W. Feinman, John G.T. Augoustides, Derek Lauter, Jillian Dashell, Elvera L. Baron, Archer Kilbourne Martin, Stuart J. Weiss, Harish Ramakrishna, Menachem M. Weiner, Kamrouz Ghadimi, Emily K. Gordon, George Silvay, Saumil Patel, Ashley V. Fritz, Ray Munroe, Arun L. Jayaraman, Adam S. Evans, Jacob T. Gutsche, Hynek Riha, Eric Guelaff, and J. Ross Renew

Subjects
Saphenous vein graft, Heart Valve Diseases, Left atrium, Coronary Artery Disease, 030204 cardiovascular system & hematology, Prosthesis Design, 03 medical and health sciences, 0302 clinical medicine, Anesthesia, Cardiac Procedures, Humans, Medicine, 030212 general & internal medicine, Paravalvular leak, Cardiac Surgical Procedures, Heart Valve Prosthesis Implantation, Heartmate ii, business.industry, Surgical risk, Anesthesiology and Pain Medicine, medicine.anatomical_structure, Anesthesia, Operative risk, Cardiology and Cardiovascular Medicine, business, Vascular Surgical Procedures, Noncardiac surgery, Surgical ablation
Published
2018
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14. Analysis of Outcomes in Lung Transplantation

Author

Andrew W. Murray, Archer Kilbourne Martin, Harish Ramakrishna, Ashley V. Fritz, Arun L. Jayaraman, and J. Ross Renew

Subjects
Lung Diseases, medicine.medical_specialty, business.industry, medicine.medical_treatment, Hypertension, Pulmonary, medicine.disease, Pulmonary hypertension, Anesthesiology and Pain Medicine, Postoperative Complications, Treatment Outcome, Internal medicine, Monitoring, Intraoperative, medicine, Lung transplantation, Humans, Cardiology and Cardiovascular Medicine, business, Respiratory Insufficiency, Lung Transplantation
Published
2018

15. The Hemodynamic Hole

Author

Theresa A. Gelzinis and Arun L. Jayaraman

Subjects
Vasopressin, Sympathetic nervous system, Aldosterone, biology, business.industry, Hemodynamics, Angiotensin-converting enzyme, Pharmacology, chemistry.chemical_compound, medicine.anatomical_structure, chemistry, Pharmacodynamics, Renin–angiotensin system, biology.protein, Medicine, Angiotensin Receptor Blockers, business
Abstract

This case discusses multiple cumulative pharmacodynamic interactions between general and neuraxial anesthetics, angiotensin converting enzyme inhibitors and angiotensin receptor blockers, resulting in significant intraoperative hypotension.

Published
2015
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17. Contributors

Author

Sanjib Adhikary, Jorge Aguilar, Charles Ahere, Moustafa Ahmed, Jane C. Ahn, Shamsuddin Akhtar, David B. Albert, Nasrin N. Aldawoodi, John T. Algren, Gracie Almeida-Chen, David Amar, Zirka H. Anastasian, Stephen Aniskevich, Solomon Aronson, Harendra Arora, Amit Asopa, Joshua H. Atkins, John G. Augoustides, Mohammad Fareed Azam, Catherine R. Bachman, Douglas R. Bacon, Andrew D. Badley, Emily Baird, Alethia Baldwin, Ryan Ball, Amir Baluch, David Bandola, Shawn Banks, Paul G. Barash, Kathleen E. Barrett, Shawn T. Beaman, Jonathan C. Beathe, Christopher D. Beatie, W. Scott Beattie, Perry S. Bechtle, G. Richard Benzinger, Lauren Berkow, Jeffrey M. Berman, Wendy K. Bernstein, Arnold J. Berry, Frederic Berry, Ulrike Berth, Walter Bethune, Sumita Bhambhani, Shobana Bharadwaj, Neil Bhatt, Frederic T. Billings, Wendy B. Binstock, David J. Birnbach, Michael Bishop, Stephanie Black, Mary A. Blanchette, James M. Blum, Krishna Boddu, Lara Bonasera, Richard L. Boortz-Marx, Cecil O. Borel, Gregory H. Botz, Charles D. Boucek, William Bradford, Jason C. Brainard, Michelle Braunfeld, Ferne R. Braveman, Caridad Bravo-Fernandez, Peter H. Breen, Marjorie Brennan, Tricia Brentjens, Megan A. Brockel, Jay B. Brodsky, Todd A. Bromberg, Adam J. Broussard, Chris Broussard, Carmen Labrie-Brown, Robert H. Brown, Charles S. Brudney, Sorin J. Brull, Claude Brunson, Trent Bryson, Jacob M. Buchowski, Stefan Budac, Zachary D. Bush, John Butterworth, Lisbeysi Calo, Christopher Canlas, Ayana Cannon, Shawn M. Cantie, Lisa Caplan, Marco Caruso, Davide Cattano, Charles B. Cauldwell, Laura Cavallone, Maurizio Cereda, Thomas M. Chalifoux, Susan Chan, Theodore G. Cheek, Alexander Chen, Samuel A. Cherry, Albert T. Cheung, Grace L. Chien, Peter T. Choi, Christopher Ciarallo, Franklyn Cladis, Anthony J. Clapcich, Richard B. Clark, Mindy Cohen, Neal H. Cohen, Robert I. Cohen, Stephan J. Cohn, Aisling Conran, Richard I. Cook, Randall F. Coombs, David M. Corda, Daniel Cormican, Darren Cousin, Vincent S. Cowell, Lyndsey Cox, Paula A. Craigo, Richard C. Cross, Roy F. Cucchiara, William H. Daily, Gaurang Dalal, Priti Dalal, Michael Danekas, Ahmed M. Darwish, Ribal Darwish, Suanne M. Daves, Kathleen Davis, Peter J. Davis, Bracken J. De Witt, Ellise Delphin, Seema Deshpande, Dawn P. Desiderio, Tricia Desvarieux, Laura K. Diaz, Christian Diez, Sanjay Dixit, Meenakshi Dogra, Karen B. Domino, Kathryn Dorhauer, Todd Dorman, Don D. Doussan, James Duke, Ann C. Duncan, Frank W. Dupont, Andrew Dziewit, L. Jane Easdown, R. Blaine Easley, Thomas J. Ebert, David M. Eckmann, Talmage D. Egan, Seth Eisdorfer, Nabil M. Elkassabany, Ryan P. Ellender, Logan S. Emory, Monique Espinosa, Lucinda L. Everett, Nauder Faraday, James J. Fehr, James M. Feld, Lynn A. Fenton, Laura H. Ferguson, Matthew Fiegel, Aaron M. Fields, Gordon N. Finlayson, Alan Finley, Gregory W. Fischer, Gary Fiskum, Molly Fitzpatrick, Russell Flatto, Lee A. Fleisher, Ronda Flower, Annette G. Folgueras, Patrick J. Forte, Joseph F. Foss, Charles J. Fox, William R. Furman, Robert Gaiser, David R. Gambling, Scott Gardiner, Matthew L. Garvey, Abraham C. Gaupp, Steven Gayer, Jeremy M. Geiduschek, Frank Gencorelli, Eric Gewirtz, Ghaleb A. Ghani, Charles P. Gibbs, Jeremy L. Gibson, Lori Gilbert, Kevin J. Gingrich, Gregory Ginsburg, Christopher Giordano, Christine E. Goepfert, Hernando Gomez, Santiago Gomez, Alanna E. Goodman, Stephanie R. Goodman, Alexandru Gottlieb, Ori Gottlieb, Allan Gottschalk, Basavana Gouda Goudra, Harry J. Gould, Nikolaus Gravenstein, Megan Graybill, William J. Greeley, Patrick Guffey, Ala Sami Haddadin, John G. Hagen, Karim Abdel Hakim, Michael Hall, N. James Halliday, Raafat S. Hannallah, Jeremy Hansen, C. William Hanson, Charles B. Hantler, Andrew P. Harris, Jonathan Hastie, Henry A. Hawney, Stephen O. Heard, James E. Heavner, James G. Hecker, Elizabeth A. Hein, Eugenie Heitmiller, Mark Helfaer, Lori B. Heller, Andrew Hemphill, Adrian Hendrickse, Frederick A. Hensley, Ian A. Herrick, Douglas Hester, Eric J. Heyer, Michael S. Higgins, Roberta Hines, Charles W. Hogue, Kenneth J. Holroyd, Natalie F. Holt, Simon J. Howell, Faisal Huda, Keith E. Hude, Hayden R. Hughes, James M. Hunter, Brad J. Hymel, James W. Ibinson, Karen E. Iles, Robert M. Insoft, Shiroh Isono, Yulia Ivashkov, Bozena R. Jachna, Anna Jankowska, Norah Janosy, Arun L. Jayaraman, Nathalia Jimenez, Judy G. Johnson, Lyndia Jones, Edmund H. Jooste, Zeev N. Kain, Maudy Kalangie, Philip L. Kalarickal, Ihab Kamel, Mia Kang, Ivan Kangrga, Ravish Kapoor, Helen W. Karl, Christopher Karsanac, Swaminathan Karthik, Jeffrey A. Katz, Alan Kaye, Adam M. Kaye, A. Murat Kaynar, Nancy B. Kenepp, Miklos D. Kertai, Mary A. Keyes, Sarah Khan, Swapnil Khoche, David Y. Kim, Jerry H. Kim, Kimberly M. King, Jeffrey Kirsch, Matthew A. Klopman, Paul R. Knight, Donald D. Koblin, W. Andrew Kofke, Vincent J. Kopp, Joseph R. Koveleskie, Courtney Kowalczyk, Valeriy V. Kozmenko, Kaylyn Krummen, Sapna R. Kudchadkar, Nathan Kudrick, Adrienne Kung, C. Dean Kurth, Robert Kyle, J. Lance LaFleur, Jason G. Lai, Kirk Lalwani, William L. Lanier, Dawn M. Larson, Richard M. Layman, Chris C. Lee, Mark J. Lema, W. Casey Lenox, Jacqueline M. Leung, Roy C. Levitt, Jerrold H. Levy, J. Lance Lichtor, Charles Lin, Sharon L. Lin, Karen S. Lindeman, Lesley Lirette, Ronald S. Litman, Qianjin Liu, Renyu Liu, Wen-Shin Liu, Justin Lockman, Stanley L. Loftness, Martin J. London, Philip D. Lumb, M. Concetta Lupa, Anne Marie Lynn, Devi Mahendran, Jeffrey Mako, Anuj Malhotra, Vinod Malhotra, Andrew M. Malinow, Mark G. Mandabach, Dennis T. Mangano, Sobia Mansoor, Inna Maranets, Jonathan B. Mark, Sinisa Markovic, H. Michael Marsh, Choendal Martin, Nicole D. Martin, Douglas Martz, Veronica A. Matei, Letha Mathews, Lynne G. Maxwell, Philip McArdle, John P. McCarren, Brenda C. McClain, Brian McClure, William A. McDade, Kathryn E. McGoldrick, Brian J. McGrath, Gregory L. McHugh, David McIlroy, Jason McKeown, Thomas M. McLoughlin, R. Yan McRae, William L. Meadow, Sameer Menda, William T. Merritt, David G. Metro, Berend Mets, Hosni Mikhaeil, David W. Miller, Jessica Miller, Mohammed Minhaj, Marek A. Mirski, Nanhi Mitter, Alexander J.C. Mittnacht, Raj K. Modak, Pierre Moine, Constance L. Monitto, Richard C. Month, Richard E. Moon, Laurel E. Moore, Roger A. Moore, Thomas A. Moore, Debra E. Morrison, Jonathan Moss, John R. Moyers, Jesse J. Muir, Adam J. Munson-Young, Stanley Muravchick, John M. Murkin, Peter Nagele, Peter A. Nagi, Daniel A. Nahrwold, Michael L. Nahrwold, Madhavi Naik, Manchula Navaratnam, Stephan P. Nebbia, Priscilla Nelson, Thai T. Nguyen, Viet Nguyen, Stavroula Nikolaidis, Zoulfira Nisnevitch, Dolores B. Njoku, Mary J. Njoku, Edward J. Norris, Omonele O. Nwokolo, Daniel Nyhan, William T. O'Byrne, Edward A. Ochroch, Andrew Oken, Nathan Orgain, Nancy E. Oriol, Pedro Orozco, Andreas M. Ostermeier, Andranik Ovassapian, Mehmet S. Ozcan, Ira Padnos, Sheela S. Pai, Nirvik Pal, Dhamodaran Palaniappan, Susan K. Palmer, Howard D. Palte, Wei Pan, Oliver Panzer, Sibi Pappachan, Anthony Passannante, Dennis A. Patel, Dilipkumar K. Patel, Kirit M. Patel, Samir Patel, Shalin Patel, Sanup Pathak, Minda L. Patt, Ronald W. Pauldine, Olga Pawelek, Tim Pawelek, Kiarash Paydar, Ronald G. Pearl, Christine Peeters-Asdourian, Padmavathi R. Perela, Charise T. Petrovitch, Patricia H. Petrozza, Dennis Phillips, Mark C. Phillips, Christine Piefer, Edgar J. Pierre, S. William Pinson, Evan G. Pivalizza, Raymond M. Planinsic, Don Poldermans, Joel M. Pomerantz, Jason E. Pope, Wanda M. Popescu, Vivian H. Porche, Jahan Porhomayon, Dmitry Portnoy, Corinne K. Postle, Paul J. Primeaux, Donald S. Prough, Ferenc Puskas, Carlos A. Puyo, Forrest Quiggle, Mary Rabb, Bronwyn R. Rae, Muhammad B. Rafique, Jesse M. Raiten, Arvind Rajagopal, Srinivasan Rajagopal, Gaurav Rajpal, Chandra Ramamoorthy, Ira J. Rampil, James G. Ramsay, James A. Ramsey, Vidya N. Rao, Joana Ratsiu, Selina Read, Ronjeet Reddy, Leila L. Reduque, David L. Reich, Karene Ricketts, Cameron Ricks, Bernhard Riedel, Jyotsna Rimal, Joseph Rinehart, James M. Riopelle, Stacey A. Rizza, Amy C. Robertson, Stephen Robinson, Peter Rock, Yillam F. Rodriguez-Blanco, Michael F. Roizen, Daniel M. Roke, Ryan Romeo, Joseph Rosa, David A. Rosen, Kathleen Rosen, Stanley H. Rosenbaum, Andrew D. Rosenberg, Andrew L. Rosenberg, Henry Rosenberg, Meg A. Rosenblatt, Steven Roth, Brian Rothman, Justin L. Rountree, Matthew J. Rowan, Marc Rozner, Ryan Rubin, Stephen M. Rupp, W. John Russell, Thomas A. Russo, Alecia L. Sabartinelli, Tetsuro Sakai, Orlando J. Salinas, Paul L. Samm, Jibin Samuel, Tor Sandven, Ted J. Sanford, Joshua W. Sappenfield, Ponnusamy Saravanan, Subramanian Sathishkumar, R. Alexander Schlichter, Eric Schnell, David L. Schreibman, Armin Schubert, Peter Schulman, Todd A. Schultz, Alan Jay Schwartz, Jamie McElrath Schwartz, Jeffrey J. Schwartz, Benjamin K. Scott, Joseph L. Seltzer, Tamas Seres, Daniel I. Sessler, Navil F. Sethna, Amar Setty, Paul W. Shabaz, Pranav Shah, Saroj Mukesh Shah, Milad Sharifpour, Joanne Shay, Jay Shepherd, Jeffrey S. Shiffrin, Marina Shindell, Daniel Siker, Richard Silverman, Brett A. Simon, Nina Singh, Ashish C. Sinha, Robert N. Sladen, Kieran A. Slevin, Tod B. Sloan, Kathleen Smith, Timothy E. Smith, Victoria Smoot, Denis Snegovskikh, Betsy Ellen Soifer, Molly Solorzano, James M. Sonner, Aris Sophocles, James A. Sparrow, Joan Spiegel, Bruce D. Spiess, Ramprasad Sripada, Stanley W. Stead, Joshua D. Stearns, Kelly Stees, Clinton Steffey, Christopher Stemland, John Stene, Christopher T. Stephens, Tracey L. Stierer, O. Jameson Stokes, Bryant W. Stolp, David F. Stowe, Ted Strickland, Suzanne Strom, Erin A. Sullivan, Michele Sumler, Dajin Sun, Lena Sun, Esther Sung, Veronica C. Swanson, Judit Szolnoki, Joe Talarico, Gee Mei Tan, Darryl T. Tang, Paul Tarasi, René Tempelhoff, John E. Tetzlaff, Alisa C. Thorne, Arlyne Thung, Vasanti Tilak, Kate Tobin, Joseph R. Tobin, Michael J. Tobin, R. David Todd, Matthew Tomlinson, Thomas J. Toung, Lien B. Tran, Minh Chau Joe Tran, Kevin K. Tremper, Sanyo Tsai, George S. Tseng, Kenneth J. Tuman, Avery Tung, Cynthia Tung, Rebecca Twersky, Mark Twite, John A. Ulatowski, Michael Urban, Manuel C. Vallejo, Andrea Vannucci, Albert J. Varon, Anasuya Vasudevan, Susheela Viswanathan, Alexander A. Vitin, Wolfgang Voelckel, Ann Walia, Russell T. Wall, Terrence Wallace, Shu-Ming Wang, David C. Warltier, Lucy Waskell, Scott Watkins, Denise Wedel, Stuart J. Weiss, Charles Weissman, Nathaen Weitzel, Gregory Weller, Gina Whitney, Robert A. Whittington, Danny Wilkerson, Nancy C. Wilkes, Michael Williams, Jimmy Windsor, Bernard Wittels, Gregory A. Wolff, Andrew K. Wong, Stacie N. Woods, A.J. Wright, Zheng Xie, Christopher C. Young, Ian Yuan, Francine S. Yudkowitz, James R. Zaidan, Paul Zanaboni, Warren M. Zapol, Angela Zimmerman, and Maurice S. Zwass

Published
2011
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18. A dynamic equilibrium between CDKs and PP2A modulates phosphorylation of pRB, p107 and p130

Author

Elena Sotillo, Ana Limón, May Truongcao, Antonia Patsialou, Brian E. Wadzinski, Xavier Graña, Judit Garriga, Girish Jayadeva, and Arun L. Jayaraman

Subjects
Phosphatase, Retinoblastoma-Like Protein p107, macromolecular substances, environment and public health, Retinoblastoma Protein, Dephosphorylation, Cyclin-dependent kinase, Cell Line, Tumor, Phosphoprotein Phosphatases, Humans, Cyclin D1, Phosphorylation, Antigens, Viral, Tumor, Molecular Biology, Binding Sites, biology, Retinoblastoma-Like Protein p130, Cell Cycle, Retinoblastoma protein, Cell Biology, Protein phosphatase 2, Cell cycle, Cyclin-Dependent Kinases, Cell biology, enzymes and coenzymes (carbohydrates), Biochemistry, Protein Biosynthesis, embryonic structures, biology.protein, biological phenomena, cell phenomena, and immunity, Developmental Biology, Protein Binding
Abstract

It is thought that G(1) cyclin/CDK mediated phosphorylation of pocket proteins from mid G(1) to mitosis is reversed via dephosphorylation in mitosis. We examined the mechanisms involved in the unexpectedly rapid dephosphorylation of the pocket proteins induced via inhibition of cellular protein synthesis by cycloheximide (CHX) as well as direct inhibition of CDKs by flavopiridol. CHX and flavopiridol-induced dephosphorylation of pocket proteins is attributable to inactivation of D-type cyclin/CDKs and G(1)/S CDKs, respectively, which unmasks a phosphatase activity that targets the three pocket proteins apparently throughout the cell cycle. Treatment of cells with phosphatase inhibitors at concentrations selective for PP2A inhibition prevents CHX and flavopiridol-mediated dephosphorylation of pocket proteins in vivo. Also, ectopic expression of SV40 small t antigen, which inhibits PP2A via disruption of trimeric PP2A holoenzymes, delays CHX-induced pocket protein dephosphorylation. Moreover, dephosphorylation of p130 and p107 in cell extracts is inhibited by concentrations of okadaic acid known to inhibit PP2A, but not PP1. Finally, the PP2A catalytic subunit (PP2A/C) specifically interacts with both p130 and p107 in quiescent cells as well as cells progressing throughout the cell cycle. Together, these results demonstrate that the overall phosphorylation state of pocket proteins is determined, at least in part, by a dynamic equilibrium between CDKs and PP2A, or a closely related PP2A-like enzyme. These findings have important implications, as cell cycle or checkpoint-dependent inhibition of CDK activities counteracted by an active PP2A should have imminent effects on the phosphorylation state and activities of pocket proteins.

Published
2004

19. 4E-binding proteins, the suppressors of eukaryotic initiation factor 4E, are down-regulated in cells with acquired or intrinsic resistance to rapamycin

Author

Peter J. Houghton, Xiongwen Zhang, Michael B. Dilling, Glen S. Germain, Franklin C. Harwood, Lorina Dudkin, and Arun L. Jayaraman

Subjects
Time Factors, Eukaryotic Initiation Factor-4E, Blotting, Western, Down-Regulation, Cell Cycle Proteins, Biology, Transfection, Biochemistry, DNA-binding protein, law.invention, Proto-Oncogene Proteins c-myc, Inhibitory Concentration 50, law, Eukaryotic initiation factor, Carcinoma, medicine, Tumor Cells, Cultured, Humans, RNA, Messenger, Eukaryotic Initiation Factors, Molecular Biology, PI3K/AKT/mTOR pathway, Adaptor Proteins, Signal Transducing, Sirolimus, Antibiotics, Antineoplastic, Dose-Response Relationship, Drug, Ribosomal Protein S6 Kinases, EIF4E, Cell Biology, Ribosomal RNA, medicine.disease, Blotting, Northern, Phosphoproteins, Molecular biology, Agar, Phenotype, Drug Resistance, Neoplasm, Mutation, Suppressor, Carrier Proteins, Cell Division, Protein Binding, Signal Transduction
Abstract

To determine whether inhibition of either the ribosomal p70 S6 kinase or eukaryotic initiation factor (eIF) 4E pathways downstream of the mammalian target of rapamycin, mTOR, contributes to rapamycin-induced growth arrest, clones of Rh30 rhabdomyosarcoma cells were selected for rapamycin resistance. Expression of c-Myc and anchorage-independent growth were enhanced in resistant cells. Resistance was unstable in each of three clones characterized. In resistant cells, as compared with parental cells, approximately 10-fold less 4E-binding protein (4E-BP) was bound to eIF4E, and total cellular 4E-BP was markedly reduced. Levels of eIF4E were unchanged. Steady-state levels of 4E-BP transcript remained unaltered, but the rate of 4E-BP synthesis was reduced in resistant cells. In cells that reverted to rapamycin sensitivity, levels of total 4E-BP returned to those of parental cells. Compared with parental cells, resistant clones had either similar or lower levels and activity of ribosomal p70 S6 kinase, but c-Myc levels were elevated in both resistant and revertant clones. Several colon carcinoma cell lines with intrinsic rapamycin resistance were found to have low 4E-BP:eIF4E ratios. In stable clones of HCT8 carcinoma engineered to overexpress 4E-BP, rapamycin sensitivity increased markedly (1000-fold) as 4E-BP expression increased. These results suggest that the 4E-BP:eIF4E ratio is an important determinant of rapamycin resistance and controls certain aspects of the malignant phenotype.

Published
2002

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