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This study examines the theoretical and experimental impact of ignition delay for electronically auto mixed diesel enginerunning on a B100 biodiesel blend. Diesel and combination of diesel and Jatropha oil methyl ester (JME) are the test fuels employed in this experiment (B100). In this operation due to help on electronic fuel mixer. To operate a CI engine with typical inoculation timing (SIT) and retarded injection timing, JME and diesel were mixed neat and in various amounts (RIT). When equated to diesel fuel, the ignition delay of biodiesel is longer. The engine's ability to complete combustion is impacted by a prolonged ignition delay. The fuel's cetane number has an inverse relationship with the fuel's ignition delay for CI engines. JME gasoline showed improvements in engine efficiency and emission characteristics. JME has greatly reduced smoke, CO, and HC emissions from diesel fuel, however it has marginally increased NOx emissions when combined with SIT. There is also a decrease in NOx emissions, which was accomplished with RIT with only a minimal impact on fuel consumption rates. We examined thermal efficiency and exhaust gas temperature after using RIT to minimize the max cylinder gas pressure and ignition delay. [ABSTRACT FROM AUTHOR]