1. Enzymatic detergent in rat peritoneum. A comparative study with physiological solution.
- Author
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Arteaga-Torres R, Escartín-Chávez M, Gutiérrez-Samperio C, Ruiz-Acosta JM, Alonso-Gallegos M, and Lerma-Alvarado R
- Subjects
- Animals, Detergents administration & dosage, Detergents pharmacology, Detergents toxicity, Drug Evaluation, Preclinical, Epithelium drug effects, Epithelium pathology, Female, Instillation, Drug, Male, Microbial Sensitivity Tests, Neovascularization, Pathologic drug therapy, Neovascularization, Pathologic etiology, Neovascularization, Pathologic prevention & control, Peritoneal Fibrosis drug therapy, Peritoneal Fibrosis etiology, Peritoneal Fibrosis prevention & control, Peritoneum blood supply, Peritoneum pathology, Peritonitis complications, Peritonitis microbiology, Quaternary Ammonium Compounds administration & dosage, Quaternary Ammonium Compounds pharmacology, Quaternary Ammonium Compounds toxicity, Random Allocation, Rats, Wistar, Sodium Chloride pharmacology, Therapeutic Irrigation, Detergents therapeutic use, Enterococcus faecalis drug effects, Escherichia coli drug effects, Escherichia coli Infections drug therapy, Gram-Positive Bacterial Infections drug therapy, Peritoneum drug effects, Peritonitis drug therapy, Quaternary Ammonium Compounds therapeutic use
- Abstract
Background: Peritoneal washing out with physiological solution with different substances added is useful in peritoneal infections, but the effect of enzymatic detergents, such as quaternary didecyl-dimethyl ammonium compounds (DDAC), used in the sterilization of surgical material is unknown. We undertook this study to determine histological changes (inflammation, fibrosis and new vessel formation) in the peritoneum of Wistar rats after the application of physiological solution or DDAC., Methods: The minimum inhibitory concentration (MIC) of DDAC for E. coli (512 μg/ml) and E. faecalis (128 μg/ml) was determined. Sixty-three Wistar rats weighing 200 ± 20 g were studied. They were divided into three groups: control: 7 rats were instilled with 3 ml of physiological solution in peritoneal cavity; groups 1 and 2 were instilled with 3 ml of MIC for E. coli and E. faecalis, respectively. These groups were divided into four subgroups of seven animals. In every rat, 1 cm(2) of peritoneum was obtained at 2, 7, 14, and 21 days for histological study with hematoxylin-eosin. Ten fields were evaluated. The data obtained were analyzed with the Mann-Whitney test., Results: There were no significant differences in inflammation, fibrosis and new vessel formation with the physiological solution vs. DDAC at 2, 7, 14, and 21 days (p >0.05), except for inflammation at 2 days in group 2 (p = 0.026), which remitted., Conclusions: There was no significant difference in changes in rat peritoneum after physiological solution or DDAC application.
- Published
- 2011