15 results on '"Arroyo Jiménez, M. M."'
Search Results
2. Distribution of peptidergic populations in the human dentate gyrus (Somatostatin [SOM-28, SOM-12] and Neuropeptide Y [NPY]) during postnatal development
- Author
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Cebada-Sánchez, S., Insausti, R., González-Fuentes, J., Arroyo-Jiménez, M. M., Rivas-Infante, E., Lagartos, M. J., Martínez-Ruiz, J., Lozano, G., and Marcos, P.
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- 2014
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Catalog
3. Vitamin transporters in mice brain with aging
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Marcos, P., primary, González-Fuentes, J., additional, Castro-Vázquez, L., additional, Lozano, M. V., additional, Santander-Ortega, M. J., additional, Rodríguez-Robledo, V., additional, Villaseca-González, N., additional, and Arroyo-Jiménez, M. M., additional more...
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- 2018
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4. PEGylated Nanoemulsions for Oral Delivery: Role of the Inner Core on the Final Fate of the Formulation
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Santander-Ortega, M. J., primary, Plaza-Oliver, M., additional, Rodríguez-Robledo, V., additional, Castro-Vázquez, L., additional, Villaseca-González, N., additional, González-Fuentes, J., additional, Cano, E. L., additional, Marcos, P., additional, Lozano, M. V., additional, and Arroyo-Jiménez, M. M., additional more...
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- 2017
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5. VITAMIN E (TOCOCHROMANOLS) FROM BARLEY CAN BE A SUCCESSFUL OPPORTUNITY IN FOOD AND PHARMACEUTICAL APPROACHES.
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Castro Vázquez, L,, Rodríguez Robledo, V., Lozano, M. V., Santander Ortega, M. J., Villaseca, N., and Arroyo Jiménez, M. M.
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VITAMIN E content of food ,TOCOTRIENOL ,BARLEY ,NEUROPROTECTIVE agents ,PHARMACEUTICAL services - Published
- 2018
6. RECOVERING OF NON-CONVENTIONAL GRAINS: EMMER TOWARDS IMPROVING TOCOPHEROL INTAKE.
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Castro Vázquez, L., Rodríguez Robledo, V., Santander Ortega, M. J., Marcos, P., González, J., and Arroyo Jiménez, M. M.
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- 2018
7. 'Cinema and therapies'. Employing movies to improve basic skills in pharmacy students
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Juan tolosa, Alonso-Moreno, C., Barbero, A. J., and Arroyo-Jiménez, M. M.
8. Interneurons in the CA1 stratum oriens expressing αTTP may play a role in the delayed-ageing Pol μ mouse model.
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Selva-Clemente J, Marcos P, González-Fuentes J, Villaseca-González N, Lagartos-Donate MJ, Insausti R, and Arroyo-Jiménez MM
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- Animals, Male, Mice, Calbindins metabolism, Calbindins genetics, Mice, Inbred C57BL, Mice, Knockout, Aging metabolism, CA1 Region, Hippocampal metabolism, Carrier Proteins metabolism, Carrier Proteins genetics, Interneurons metabolism, Parvalbumins metabolism
- Abstract
Neurodegeneration associated with ageing is closely linked to oxidative stress (OS) and disrupted calcium homeostasis. Some areas of the brain, like the hippocampus - particularly the CA1 region - have shown a high susceptibility to age-related changes, displaying early signs of pathology and neuronal loss. Antioxidants such as α-tocopherol (αT) have been effective in mitigating the impact of OS during ageing. αT homeostasis is primarily regulated by the α-tocopherol transfer protein (αTTP), which is widely distributed throughout the brain - where it plays a crucial role in maintaining αT levels within neuronal cells. This study investigates the distribution of αTTP in the hippocampus of 4- and 24-month-old Pol μ knockout mice (Pol μ
-/- ), a delayed-ageing model, and the wild type (Pol μ+/+ ). We also examine the colocalisation in the stratum oriens (st.or) of CA1 region with the primary interneuron populations expressing calcium-binding proteins (CBPs) (calbindin (CB), parvalbumin (PV), and calretinin (CR)). Our findings reveal that αTTP immunoreactivity (-IR) in the st.or of Pol μ mice is significantly reduced. The density of PV-expressing interneurons (INs) increased in aged mice in both Pol μ genotypes (Pol μ-/- and Pol μ+/+ ), although the density of PV-positive INs was lower in the aged Pol μ-/- mice compared to wild-type mice. By contrast, CR- and CB-positive INs in Pol μ mice remained unchanged during ageing. Furthermore, double immunohistochemistry reveals the colocalisation of αTTP with CBPs in INs of the CA1 st.or. Our study also shows that the PV/αTTP-positive IN population remains unchanged in all groups. A significant decrease of CB/αTTP-positive INs in young Pol μ-/- mice has been detected, as well as a significant increase in CR/αTTP-IR in older Pol μ-/- animals. These results suggest that the differential expression of αTTP and CBPs could have a crucial effect in aiding the survival and maintenance of the different IN populations in the CA1 st.or, and their coexpression could contribute to the enhancement of their resistance to OS-related damage and neurodegeneration associated with ageing., Competing Interests: Declaration of competing interest The authors have no conflicts of interest to declare., (Copyright © 2024. Published by Elsevier Inc.) more...- Published
- 2024
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9. Ascorbyl-dipalmitate-stabilised nanoemulsions as a potential localised treatment of inflammatory bowel diseases.
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Plaza-Oliver M, Beloqui A, Santander-Ortega MJ, Castro-Vázquez L, Rodríguez-Robledo V, Arroyo-Jiménez MM, Préat V, and Lozano MV
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- Antioxidants administration & dosage, Antioxidants pharmacology, Biological Transport, Caco-2 Cells, Curcumin pharmacology, Drug Carriers chemistry, Drug Delivery Systems, Emulsions, Humans, Lecithins chemistry, Nanoparticles, Particle Size, Reactive Oxygen Species metabolism, Solubility, alpha-Tocopherol pharmacology, Ascorbic Acid chemistry, Curcumin administration & dosage, Inflammatory Bowel Diseases drug therapy, Palmitates chemistry, alpha-Tocopherol administration & dosage
- Abstract
Current efforts on inflammatory bowel diseases (IBD) treatment are focused on strategies for localised drug delivery at the intestinal mucosa. Despite the potential of curcumin (CC) for IBD treatment, its low solubility and stability limit its application. Thus, the design of nanocarriers that focus CC delivery at the intestinal epithelium is an area of interest. This work proposes α-tocopherol nanoemulsions (NE) stabilised by ascorbyl-2,6-dipalmitate (ADP) as intestinal CC-carriers. The antioxidant capacity of α-tocopherol and ADP could have a synergistic effect on IBD-affected tissues, characterised by an oxidative environment. We obtained nanoemulsions (NE-ADP) with size below 200 nm, negative surface charge, stable in gastrointestinal media and no toxic in the Caco-2 cell model. Intracellular retention of NE-ADP in Caco-2 cells was observed by confocal microscopy. The extremely low P
app values obtained for CC and α-tocopherol indicated the lack of transport across the Caco-2 monolayer. Control nanoemulsion stabilised by lecithin (NE-L) was greatly transported across the Caco-2 cells monolayer, confirming the relevance of ADP on the cellular retention of NE-ADP. The therapeutic potential of NE-ADP was shown by the significant decrease of intracellular ROS levels. Altogether, these results indicate the potential of NE-ADP as a novel approach for the treatment of IBD., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier B.V. All rights reserved.) more...- Published
- 2020
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10. The role of the intestinal-protein corona on the mucodiffusion behaviour of new nanoemulsions stabilised by ascorbyl derivatives.
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Plaza-Oliver M, Santander-Ortega MJ, Castro-Vázquez L, Rodríguez-Robledo V, González-Fuentes J, Marcos P, Lozano MV, and Arroyo-Jiménez MM
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- Ascorbic Acid chemistry, Diffusion, Emulsions chemistry, Particle Size, Surface Properties, alpha-Tocopherol chemistry, Ascorbic Acid analogs & derivatives, Intestines chemistry, Nanoparticles chemistry, Protein Corona chemistry
- Abstract
Nanoemulsions are vesicular systems with great potential for the delivery of drugs, which significantly depends on the appropriate selection of the components that constitute them. In this sense, the use of materials with adequate toxicity profiles for the oral route provides additional advantages in terms of safety concerns avoidance. This work describes the formulation of novel two-component nanoemulsions constituted by α-tocopherol and ascorbyl-palmitate derivatives. Among them, ascorbyl-dipalmitate allowed the formation of nanoemulsions with size values around 170 nm and negative charge; additionally, they showed strong antioxidant capacity. These nanoemulsions are proposed to the oral route, so their behaviour in intestinal conditions was evaluated by incubating the nanoemulsion in simulated intestinal fluid. This process led to the formation of an intestinal-protein corona (I-PC) at the colloidal surface that determined the interaction with the mucus barrier. The I-PC displaced the immobile-hindered particles towards a subdiffusive-diffusive population. These studies report for the first time the effect of the I-PC on the mucodiffusion behaviour of vesicular systems, a finding that may help to comprehend the performance of nanocarriers under intestinal conditions., (Copyright © 2019 Elsevier B.V. All rights reserved.) more...
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- 2020
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11. Improving green enrichment of virgin olive oil by oregano. Effects on antioxidants.
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Peñalvo GC, Robledo VR, Callado CS, Santander-Ortega MJ, Castro-Vázquez L, Lozano MV, and Arroyo-Jiménez MM
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- Cinnamates analysis, Depsides analysis, Hydroxybenzoates analysis, Plant Leaves chemistry, Rosmarinic Acid, Antioxidants analysis, Food Additives analysis, Olive Oil chemistry, Origanum chemistry, Plant Extracts analysis
- Abstract
This work is about improvement of a maceration method in order to achieve a green process for the enrichment of virgin olive oil (VOO) with natural antioxidants, specifically from oregano leaves. This goal was accomplished after evaluating different mechanical methods, i.e. magnetic stirring, sonication, vertical stirring and sonication in combination with vertical stirring, for promoting the extraction of the antioxidants from oregano. The results obtained indicated that the best extraction procedure was vertical stirring at 1000 r.p.m. for 3 h. Therefore, these conditions were selected to enrich VOO with phenolic acids (mainly rosmarinic acid) and endogenous antioxidants (o-coumaric and vanillic acids), and further determine their stability at room temperature or under temperature stress (50°C) during 45 days. Quantitative analysis of rosmarinic, o-coumaric and vanillic acids was carried out by an off-line, solid phase extraction, capillary zone, electrophoresis method combined with diode-array detector (SPE-CE-DAD)., (Copyright © 2015 Elsevier Ltd. All rights reserved.) more...
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- 2016
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12. Developmental study of vitamin C distribution in children's brainstems by immunohistochemistry.
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Coveñas R, González-Fuentes J, Rivas-Infante E, Lagartos-Donate MJ, Mangas A, Geffard M, Arroyo-Jiménez MM, Cebada-Sánchez S, Insausti R, and Marcos P
- Subjects
- Antioxidants metabolism, Child, Child, Preschool, Female, Humans, Immunohistochemistry, Infant, Infant, Newborn, Locus Coeruleus metabolism, Male, Nerve Fibers metabolism, Neurons metabolism, Ascorbic Acid metabolism, Brain Stem growth & development, Brain Stem metabolism
- Abstract
Vitamin C (Vit C) is an important antioxidant, exerts powerful neuroprotective brain effects and plays a role in neuronal development and maturation. Vit C is present in brain tissue at higher concentrations than in other organs, but its detailed distribution in brain is unknown. Immunohistochemical detection of this vitamin has been performed by using a highly specific antibody against Vit C. The aim of the present work was to analyze the distribution of Vit C in children's brainstems during postnatal development, comparing two groups of ages: younger and older than one year of life. In general, the same areas showing neurons with Vit C in young cases are also immunostained at older ages. The distribution of neurons containing Vit C was broader in the brainstems of older children, suggesting that brainstem neurons maintain or even increase their ability to retain Vit C along the life span. Immunohistochemical labeling revealed only cell bodies containing this vitamin, and no immunoreactive fibers were observed. The distribution pattern of Vit C in children's brainstems suggests a possible role of Vit C in brain homeostatic regulation. In addition, the constant presence of Vit C in neurons of locus coeruleus supports the important role of Vit C in noradrenaline synthesis, which seemed to be maintained along postnatal development., (Copyright © 2015 Elsevier GmbH. All rights reserved.) more...
- Published
- 2015
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13. Developmental study of the distribution of hypoxia-induced factor-1 alpha and microtubule-associated protein 2 in children's brainstem: comparison between controls and cases with signs of perinatal hypoxia.
- Author
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Coveñas R, González-Fuentes J, Rivas-Infante E, Lagartos-Donate MJ, Cebada-Sánchez S, Arroyo-Jiménez MM, Insausti R, and Marcos P
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- Brain Stem pathology, Child, Child, Preschool, Female, Humans, Hypoxia pathology, Hypoxia, Brain pathology, Hypoxia-Ischemia, Brain metabolism, Hypoxia-Ischemia, Brain pathology, Immunohistochemistry, Infant, Infant, Newborn, Male, Medulla Oblongata growth & development, Medulla Oblongata metabolism, Medulla Oblongata pathology, Neurons metabolism, Neurons pathology, Photomicrography, Pons growth & development, Pons metabolism, Pons pathology, Solitary Nucleus growth & development, Solitary Nucleus metabolism, Solitary Nucleus pathology, Brain Stem growth & development, Brain Stem metabolism, Hypoxia metabolism, Hypoxia, Brain metabolism, Hypoxia-Inducible Factor 1, alpha Subunit metabolism, Microtubule-Associated Proteins metabolism
- Abstract
Perinatal asphyxia and hypoxia are common causes of morbidity in neonates. Prenatal birth associated with hypoxemia often results in several disorders because of the lack of oxygen in the brain. Survival rates from perinatal hypoxia have improved, but appropriate treatments for recovery are still limited, with great impact on patients, their families, society in general and health systems. The aim of this work is to contribute to a better understanding of the cellular mechanisms underlying the brainstem responses to hypoxia. For this purpose, distributions of two proteins, hypoxia-inducible factor-1 alpha (HIF-1α) and microtubule-associated protein 2 (MAP-2) were analyzed in brainstems of 11 children, four of them showing neuropathological evidence of brain hypoxia. They were included in control or hypoxic groups, and then in several subgroups according to their age. Immunohistochemical labeling for these proteins revealed only cell bodies containing HIF-1α, and both cell bodies and fibers positive for MAP-2 in the children's brainstems. The distribution of HIF-1α was more restricted than that of MAP-2, and it can be suggested that the expression of HIF-1α increased with age. The distribution pattern of MAP-2 in the medulla oblongata could be more due to age-related changes than to a response to hypoxic damage, whereas in the pons several regions, such as the nucleus ambiguus or the solitary nucleus, showed different immunolabeling patterns in controls and hypoxic cases. The distribution patterns of these two proteins suggest that some brainstem regions, such as the reticular formation or the central gray, could be less affected by conditions of hypoxia., (Copyright © 2014 IBRO. Published by Elsevier Ltd. All rights reserved.) more...
- Published
- 2014
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14. Mapping of tyrosine hydroxylase in the diencephalon of alpaca (Lama pacos) and co-distribution with somatostatin-28 (1-12).
- Author
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Marcos P, Arroyo-Jiménez MM, Lozano G, González-Fuentes J, Lagartos-Donate MJ, Aguilar LA, and Coveñas R
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- Animals, Immunohistochemistry, Male, Peptide Fragments biosynthesis, Somatostatin-28 biosynthesis, Tyrosine 3-Monooxygenase biosynthesis, Camelids, New World metabolism, Diencephalon metabolism, Neurons metabolism, Peptide Fragments analysis, Somatostatin-28 analysis, Tyrosine 3-Monooxygenase analysis
- Abstract
Based on previous work describing the distribution of somatostatin-28 (1-12) in the male alpaca (Lama pacos) diencephalon, and owing to the well known interactions between this peptide and the catecholaminergic system, the aims of this work are (1) to describe the distribution of putative catecholaminergic cell groups in the alpaca diencephalon and (2) to study the possible morphological basis of the interactions between these substances in the diencephalon of the alpaca by using double immunohistochemistry methods. Thus, the distribution of catecholaminergic cell groups in the alpaca diencephalon agrees with that previously described in the diencephalon of other mammalian species of the same order: the A11, A12, A13, A14 and A15d cell groups have been identified; however, we have observed an additional hitherto undescribed cell group containing tyrosine hydroxylase in the medial habenula. In addition, double-labelling procedures did not reveal neurons containing tyrosine hydroxylase and somatostatin, suggesting that the hypothalamic interactions between catecholamines and somatostatin at intra-cellular level must be carried out by a somatostatin molecule other than fragment (1-12). Otherwise, the overlapping distribution patterns of these substances would suggest some interconnections between groups of chemospecific neurons. These results could be the starting point for future studies on hypothalamic functions in alpacas, for example those concerning reproductive control, since other physiological studies have suggested that this species could have different regulatory mechanisms from other mammalian species. Our results support the Manger hypothesis that the same nuclear complement of neural systems exists in the brain of species of the same order., (Copyright © 2013 Elsevier B.V. All rights reserved.) more...
- Published
- 2013
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15. Comparative aspects of the olfactory portion of the entorhinal cortex and its projection to the hippocampus in rodents, nonhuman primates, and the human brain.
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Insausti R, Marcos P, Arroyo-Jiménez MM, Blaizot X, and Martínez-Marcos A
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- Animals, Entorhinal Cortex anatomy & histology, Entorhinal Cortex cytology, Humans, Neural Pathways physiology, Olfactory Pathways anatomy & histology, Olfactory Pathways cytology, Entorhinal Cortex physiology, Hippocampus physiology, Macaca fascicularis physiology, Olfactory Pathways physiology, Rats physiology, Synaptic Transmission physiology
- Abstract
The entorhinal cortex is a component of the hippocampal formation characterized by its projection to the dentate gyrus. Cortical input is relayed to the hippocampus through the entorhinal cortex. The entorhinal cortex can be recognized best in mammals, and it reaches its maximal importance in both human and nonhuman primates. In primates, but not in rodents, the olfactory projection from the olfactory bulb is the only direct sensory projection reaching the entorhinal cortex, where it defines a rostromedial subfield (olfactory subfield of the entorhinal cortex, EO). In this communication, we consider some comparative aspects of this projection in rodents, nonhuman primates, and human brains, in relation to the forward projection to the hippocampus. We base our analysis on the following three considerations: (1) Topology: Topologically, the entorhinal cortex lies medial to the rhinal fissure, well developed in all mammals except in humans. EO maintains the same topological relationship to the rhinal fissure in the three mammalian orders. (2) Cytoarchitecture: The rostromedial portion shows poorer lamination compared with caudomedial portions of the entorhinal cortex in the three mammalian orders examined. (3) Connectivity: In rodents, the olfactory projection covers the whole extent of the entorhinal cortex, whereas it decreases substantially in macaques, where it roughly covers 15% of the entorhinal cortex. The human EO as defined by topological and cytoarchitectonic criteria may be even less than in macaques. Interestingly, good evidence exists that all of these mammalian orders maintain the same topographical scheme of projection to the rostral end of the hippocampus. more...
- Published
- 2002
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