Search

Your search keyword '"Arnold, Markus F.F."' showing total 2 results
Start Over Author "Arnold, Markus F.F."
2 results on '"Arnold, Markus F.F."'

1. Internalization of a thiazole-modified peptide in Sinorhizobium meliloti occurs by BacA-dependent and -independent mechanisms

Author

Wehmeier, Silvia, Arnold, Markus F.F., Marlow, Victoria L., Aouida, Mustapha, Myka, Kamila K., Fletcher, Vivien, Benincasa, Monica, Scocchi, Marco, Ramotar, Dindial, and Ferguson, Gail P.

Subjects
Bacterial infections -- Research, Bacterial proteins -- Physiological aspects, Bacterial proteins -- Research, Drug resistance in microorganisms -- Research, Biological sciences
Abstract

BacA proteins play key roles in the chronic intracellular infections of Sinorhizobium meliloti, Brucella abortus and Mycobacterium tuberculosis within their respective hosts. S. meliloti, B. abortus and M. tuberculosis BacA-deficient mutants have increased resistance to the thiazole-modified peptide bleomycin. BacA has been previously hypothesized, but not experimentally verified, to be involved in bleomycin uptake. In this paper, we show that a BacA-dependent mechanism is the major route of bleomycin internalization in S. meliloti. We also determined that the B. abortus and S. meliloti BacA proteins are functional homologues and that the B. abortus BacA protein is involved in the uptake of both bleomycin and proline-rich peptides. Our findings also provide evidence that there is a second, BacA-independent minor mechanism for bleomycin internalization in S. meliloti. We determined that the BacA-dependent and -independent mechanisms of bleomycin uptake are energy-dependent, consistent with both mechanisms of bleomycin uptake involving transport systems. DOI 10.1099/mic.0.039909-0

Published
2010

2. Importance of lipopolysaccharide and cyclicβ-1,2-Glucans in Brucella-mammalian infections

Author

Haag, Andreas F., Myka, Kamila K., Arnold, Markus F.F., Caro-Hernández, Paola, and Ferguson, Gail P.

Abstract

Brucella species are the causative agents of one of the most prevalent zoonotic diseases: brucellosis. Infections by Brucella species cause major economic losses in agriculture, leading to abortions in infected animals and resulting in a severe, although rarely lethal, debilitating disease in humans. Brucella species persist as intracellular pathogens that manage to effectively evade recognition by the host's immune system. Sugar-modified components in the Brucella cell envelope play an important role in their host interaction. Brucella lipopolysaccharide (LPS), unlike Escherichia coli LPS, does not trigger the host's innate immune system. Brucella produces cyclic -1,2-glucans, which are important for targeting them to their replicative niche in the endoplasmic reticulum within the host cell. This paper will focus on the role of LPS and cyclic -1,2-glucans in Brucella-mammalian infections and discuss the use of mutants, within the biosynthesis pathway of these cell envelope structures, in vaccine development.

Published
2010

Catalog

Books, media, physical & digital resources
See catalog results