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This project is critically situated within the Melbourne urban environment. Encounters within the proximate of my everyday environment form the basis from which sculptural works are generated. Research has been undertaken in many ways include walking, studio and installation based experimentation, and investigation of other artists who are critically situated within the urban environment. This paper establishes the key considerations of this project, including mapping, fragmentation, transformation, and the ordinary. These discussions lead to an expanded understanding of site as an experienced situation. Claire Doherty’s term ‘situation-specific’ is explored, and has heavily informed this position. Section One explores the Situationist International and their projects that re-constructed the city through processes of mapping. This section considers mapping as a means to explore cities and the act of walking as an embodied methodology for this process. The Situationists provide an historical context for approaching urban environments as a shifting situation composed of multiple relations, introducing notions of fragmentation to the project. Discussion of the Situationists within this section provides an engagement with site, offering an opportunity to introduce my own approach to site developed throughout this project. Section Two engages in a discussion around my own processes employed throughout the project to investigate and experience site. Walking is established as a key approach, assisting an understanding of site that is multilayered and temporal rather than fixed. Part of my encounter with site involved the formation of collections; these collections of photographs and sculptural moulds form the basis of the studio component. The art practices of Francis Alÿs and Lucy Pedlar inform this section for the particular ways in which they generate work in response to their immediate environments. Engaging in a discussion of their work, the paper explores how their p

In previous studies we evaluated the incidence and specificity of autologous antibody reactivity against squamous cell carcinoma of the head and neck (SCCHN). We were able to demonstrate that autologous antibody reactivity is present in native sera but was usually of too low a titer to allow further analysis. Dissociation of immune complexes by acidification and ultrafiltration of serum augmented autologous antibody reactivity in nine out of nine autologous systems tested. Native antibody and antibody derived from immune complexes produced by the host and reactive with autologous tumor cells may be directed against physiologically relevant antigens. Therefore, correlations of antibody titers with clinical course may provide insight into the nature of the host response to cancer. In the present analysis, serological studies of six patients with SCCHN were performed with serum samples obtained over many months. Results of serial serological assays were correlated to tumor progression and clinical course. Fluctuations in autologous antibody reactivity were noted over time. In four cases, rises in autologous antibody titers preceded the clinical diagnosis of recurrence by several months. Drops in autologous antibody reactivity were noted in two cases following surgery or radiation therapy. In two cases of long-term survivors, no correlation between antibody reactivity and clinical course was noted. Specificity analysis of the six autologous systems demonstrated reactivity against autologous and allogeneic SCCHN as well as melanoma cell lines. These sera did not react with glioma, neuroblastoma, renal cell, breast, bladder and colon carcinoma cell lines nor with fetal calf serum, pooled lymphocytes, red blood cells and platelets. Autologous serial serological studies may provide a means by which to evaluate the host/tumor relationship in patients with SCCHN.

We have previously shown that detection of autologous antibody activity to squamous cell carninoma of the head and neck many be augmented by dissociation in immune complexes. Western blot analysis with autologous antibody has identified a 60 kDa squamous cell carcinoma of the head and neck-associated antigen in spent media and immune complex-dissociated serum ultrafiltrate not recognized by normal human area. Antigen-containing fractions of spent media were eluted from anion exchange columns immediately after serum albumin indicating that the antigen has an acidic PI Triticum vulgaris column. A single 60 kDa band was detected by silver stain and Western blot in antigen-containing fractions eluted following lectin affinity chromotography and SDS-PAGE. Final concentration of the antigen was determined to be 1 [mu]m/ml of protein with relative activity increased 1600 x over unfractionated spent media. We conclude that a squamous cell carcinoma of the head and neck-associated antigen, detected by autologous antibody, is an acidic kDa glycoprotein.