19 results on '"Armstrong, JP"'
Search Results
2. A mixed methods investigation of the relationship between blood donor policy, interest in donation, and willingness to donate among gay, bisexual, and other men who have sex with men in Ontario, Canada
- Author
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Armstrong, JP, primary, Brennan, David J., primary, Collict, David, primary, Kesler, Maya, primary, Bekele, Tsegaye, primary, Souleymanov, Rusty, primary, Grace, Daniel, primary, Lachowsky, Nathan J., primary, Hart, Trevor, primary, and Adam, Barry D., primary
- Published
- 2024
- Full Text
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3. Effects of dichlorvos, maldison and pirimiphos-methyl on food consumption, egg production, egg and tissue residues, and plasma acetylcholinesterase inhibition in layer strain hens
- Author
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Pym, RAE, Singh, G, Gilbert, WS, Armstrong, JP, and McCleary, BV
- Abstract
In three experiments laying performance was studied in hens given graded levels of maldison, dichlorvos and pirimiphos-methyl either separately or combined in the feed over a four-week test period. Results conclusively demonstrated interaction between dichlorvos and maldison as measured by depressed food consumption and egg production. Combining the three insecticides at levels which when given separately had no effect, severely depressed food consumption and egg production. After four weeks on treatment, birds receiving pirimiphos-methyl at 50 g/g of diet had residues of 0.08-0.17 g/g in fat and 0-0.06 g/g in muscle, and residues of 0-0.07 g/g maldison were recovered in the fat of birds receiving it at 100 g/g of diet. No residues of any insecticide were detected in eggs and no dichlorvos residues were detected in any tissues. Plasma acetylcholinesterase (AChE) levels were reduced by 70% with dichlorvos at 30 g/g, by 30% with maldison at 100 g/g and by 90% with pirimiphos-methyl at 50 g/g. There was no indication of potentiation between insecticides as measured by plasma AChE inhibition, and effects upon food consumption and egg production appeared unrelated to plasma AChE activity. The relationship between food consumption and egg production was similar in groups receiving dichlorvos-maldison mixtures and in those receiving graded levels of untreated food, indicating that the insecticides' effect upon egg production was mediated via a reduced food intake. Maximum residue limits for pesticides in feeds should be based on a total index which takes account of interaction between the different pesticides present.
- Published
- 1984
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4. Using intentional emergence to dethrone the sage on the stage.
- Author
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Armstrong JP and Vickers AM
- Subjects
- Humans, Students, Curriculum, Group Processes, Leadership, Learning
- Abstract
Intentional emergence (IE) as a pedagogy centers students in learning and calls for the educator or facilitator to take a different role. It is important for educators to mindfully regulate their presence in the classroom to allow students to notice the role of authority in leadership practice. This article provides recommendations for productive learning when authority is de-centered and learners are encouraged to take up their authority. Facilitators who reflect and consider their identities and the identities of their participants will be more prepared for what emerges in the classroom. Finally, educators center student learning by intentionally creating a safe container before giving back the work to students in meaningful ways, allowing themselves to take a backseat and observe students exercising leadership., (© 2024 Wiley Periodicals, LLC.)
- Published
- 2024
- Full Text
- View/download PDF
5. Willingness and eligibility to donate blood under 12-month and 3-month deferral policies among gay, bisexual, and other men who have sex with men in Ontario, Canada.
- Author
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Brennan DJ, Armstrong J, Kesler M, Bekele T, Lachowsky NJ, Grace D, Hart TA, Souleymanov R, and Adam BD
- Abstract
In Canada, gay, bisexual and other men who have sex with men (GBMSM) are a population that are willing to donate blood, if eligible, but have a history of ineligibility and deferrals due to concerns that their blood poses an increased risk of HIV entering the blood supply. Our objective was to examine the proportion of GBMSM who are willing and eligible to donate under the 12-month deferral policy (implemented in 2016) and the 3-month deferral policy (implemented in 2019). Data for this study comes from the #iCruise study, a mixed cohort study designed to examine sexual health outreach experiences through online services and mobile apps among GBMSM in Ontario. A total of 910 participants were recruited between July 2017 and January 2018. Eligibility criteria include identify as male (cisgender or transgender); at least 14 years old; having had sex with a man in the previous year or identifying as sexually/romantically attracted to other men or identifying as gay, bisexual, queer or two-spirit; and living or working in Ontario or having visited Ontario four or more times in the past year. Participants completed a baseline and a follow-up questionnaire. A subset of #iCruise participants (n = 447) further completed this questionnaire. Willingness and eligibility to donate blood were assessed under 12-month and 3-month deferral policies. Of the 447 GBMSM surveyed, 309 (69.1%) reported a general interest in donating blood. 109 (24.4%) GBMSM were willing, 75 (16.7%) were eligible, and 24 (5.4%) were both willing and eligible to donate blood under the 12-month deferral policy. Under the 3-month deferral policy, willingness and eligibility to donate blood increased significantly to 42.3% and 29.3%, respectively. The percent of GBMSM who were both willing and eligible to donate blood also increased significantly to 12.3% under the 3-month deferral policy. The increase in willingness to donate blood varied by age, ethnicity, and geographic residence of participants whereas the increase in eligibility to donate blood varied by education level of participants. Under the 3-month deferral policy, GBMSM who were 50 years or older, identified as bisexual or other, had a lower education level, and who were not 'out' to others were more likely to be eligible to donate. GBMSM who reported a general interest in donating blood were more likely to be willing to donate blood under both deferral policies. The most common reason for not being interested in donating blood was the MSM deferral policy itself; many participants interpreted the policy as discriminatory for 'singling out' GBMSM or self-assed themselves as ineligible. Among study participants, both willingness and eligibility to donate blood was significantly higher under the 3-month deferral policy. The results suggest that a time-based reduction to a 3-month deferral policy is impactful but limited. Future research should measure GBMSM's willingness and eligibility under the individual risk-based assessment (to be implemented in 2022)., Competing Interests: The authors have declared that no competing interests exist., (Copyright: © 2023 Brennan et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
- Published
- 2023
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6. Plume-driven recratonization of deep continental lithospheric mantle.
- Author
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Liu J, Pearson DG, Wang LH, Mather KA, Kjarsgaard BA, Schaeffer AJ, Irvine GJ, Kopylova MG, and Armstrong JP
- Abstract
Cratons are Earth's ancient continental land masses that remain stable for billions of years. The mantle roots of cratons are renowned as being long-lived, stable features of Earth's continents, but there is also evidence of their disruption in the recent
1-6 and more distant7-9 past. Despite periods of lithospheric thinning during the Proterozoic and Phanerozoic eons, the lithosphere beneath many cratons seems to always 'heal', returning to a thickness of 150 to 200 kilometres10-12 ; similar lithospheric thicknesses are thought to have existed since Archaean times3,13-15 . Although numerous studies have focused on the mechanism for lithospheric destruction2,5,13,16-19 , the mechanisms that recratonize the lithosphere beneath cratons and thus sustain them are not well understood. Here we study kimberlite-borne mantle xenoliths and seismology across a transect of the cratonic lithosphere of Arctic Canada, which includes a region affected by the Mackenzie plume event 1.27 billion years ago20 . We demonstrate the important role of plume upwelling in the destruction and recratonization of roughly 200-kilometre-thick cratonic lithospheric mantle in the northern portion of the Slave craton. Using numerical modelling, we show how new, buoyant melt residues produced by the Mackenzie plume event are captured in a region of thinned lithosphere between two thick cratonic blocks. Our results identify a process by which cratons heal and return to their original lithospheric thickness after substantial disruption of their roots. This process may be widespread in the history of cratons and may contribute to how cratonic mantle becomes a patchwork of mantle peridotites of different age and origin.- Published
- 2021
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7. Glycosylated superparamagnetic nanoparticle gradients for osteochondral tissue engineering.
- Author
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Li C, Armstrong JP, Pence IJ, Kit-Anan W, Puetzer JL, Correia Carreira S, Moore AC, and Stevens MM
- Subjects
- Bone Morphogenetic Protein 2 metabolism, Cell Differentiation, Cell Survival, Cells, Cultured, Drug Carriers, Drug Liberation, Electromagnetic Fields, Glycosylation, Humans, Osteogenesis, Sepharose chemistry, Tissue Engineering methods, Biocompatible Materials chemistry, Cartilage cytology, Hydrogels chemistry, Magnetite Nanoparticles chemistry, Mesenchymal Stem Cells cytology, Tissue Scaffolds chemistry
- Abstract
In developmental biology, gradients of bioactive signals direct the formation of structural transitions in tissue that are key to physiological function. Failure to reproduce these native features in an in vitro setting can severely limit the success of bioengineered tissue constructs. In this report, we introduce a facile and rapid platform that uses magnetic field alignment of glycosylated superparamagnetic iron oxide nanoparticles, pre-loaded with growth factors, to pattern biochemical gradients into a range of biomaterial systems. Gradients of bone morphogenetic protein 2 in agarose hydrogels were used to spatially direct the osteogenesis of human mesenchymal stem cells and generate robust osteochondral tissue constructs exhibiting a clear mineral transition from bone to cartilage. Interestingly, the smooth gradients in growth factor concentration gave rise to biologically-relevant, emergent structural features, including a tidemark transition demarcating mineralized and non-mineralized tissue and an osteochondral interface rich in hypertrophic chondrocytes. This platform technology offers great versatility and provides an exciting new opportunity for overcoming a range of interfacial tissue engineering challenges., (Copyright © 2018 The Authors. Published by Elsevier Ltd.. All rights reserved.)
- Published
- 2018
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8. Re-Engineering Extracellular Vesicles as Smart Nanoscale Therapeutics.
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Armstrong JP, Holme MN, and Stevens MM
- Subjects
- Animals, Extracellular Vesicles metabolism, Humans, Hydrophobic and Hydrophilic Interactions, Nanomedicine, Peptides administration & dosage, Peptides chemistry, Pharmaceutical Preparations administration & dosage, Pharmaceutical Preparations chemistry, RNA administration & dosage, RNA chemistry, Drug Delivery Systems methods, Extracellular Vesicles chemistry, Nanoparticles chemistry
- Abstract
In the past decade, extracellular vesicles (EVs) have emerged as a key cell-free strategy for the treatment of a range of pathologies, including cancer, myocardial infarction, and inflammatory diseases. Indeed, the field is rapidly transitioning from promising in vitro reports toward in vivo animal models and early clinical studies. These investigations exploit the high physicochemical stability and biocompatibility of EVs as well as their innate capacity to communicate with cells via signal transduction and membrane fusion. This review focuses on methods in which EVs can be chemically or biologically modified to broaden, alter, or enhance their therapeutic capability. We examine two broad strategies, which have been used to introduce a wide range of nanoparticles, reporter systems, targeting peptides, pharmaceutics, and functional RNA molecules. First, we explore how EVs can be modified by manipulating their parent cells, either through genetic or metabolic engineering or by introducing exogenous material that is subsequently incorporated into secreted EVs. Second, we consider how EVs can be directly functionalized using strategies such as hydrophobic insertion, covalent surface chemistry, and membrane permeabilization. We discuss the historical context of each specific technology, present prominent examples, and evaluate the complexities, potential pitfalls, and opportunities presented by different re-engineering strategies.
- Published
- 2017
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9. Synthesis of Cationized Magnetoferritin for Ultra-fast Magnetization of Cells.
- Author
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Correia Carreira S, Armstrong JP, Okuda M, Seddon AM, Perriman AW, and Schwarzacher W
- Subjects
- Animals, Apoferritins chemistry, Horses, Humans, Iron chemistry, Mesenchymal Stem Cells metabolism, Magnetite Nanoparticles chemistry, Mesenchymal Stem Cells cytology, Staining and Labeling methods
- Abstract
Many important biomedical applications, such as cell imaging and remote manipulation, can be achieved by labeling cells with superparamagnetic iron oxide nanoparticles (SPIONs). Achieving sufficient cellular uptake of SPIONs is a challenge that has traditionally been met by exposing cells to elevated concentrations of SPIONs or by prolonging exposure times (up to 72 hr). However, these strategies are likely to mediate toxicity. Here, we present the synthesis of the protein-based SPION magnetoferritin as well as a facile surface functionalization protocol that enables rapid cell magnetization using low exposure concentrations. The SPION core of magnetoferritin consists of cobalt-doped iron oxide with an average particle diameter of 8.2 nm mineralized inside the cavity of horse spleen apo-ferritin. Chemical cationization of magnetoferritin produced a novel, highly membrane-active SPION that magnetized human mesenchymal stem cells (hMSCs) using incubation times as short as one minute and iron concentrations as lows as 0.2 mM.
- Published
- 2016
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10. Effect of Bioconjugation on the Reduction Potential of Heme Proteins.
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Risbridger TA, Watkins DW, Armstrong JP, Perriman AW, Anderson JL, and Fermin DJ
- Subjects
- Anions chemistry, Arsenicals chemistry, Circular Dichroism, Oxidation-Reduction, Protein Conformation drug effects, Protein Denaturation, Static Electricity, Temperature, Water chemistry, Cytochromes c chemistry, Heme chemistry, Myoglobin chemistry
- Abstract
The modification of protein surfaces employing cationic and anionic species enables the assembly of these biomaterials into highly sophisticated hierarchical structures. Such modifications can allow bioconjugates to retain or amplify their functionalities under conditions in which their native structure would be severely compromised. In this work, we assess the effect of this type of bioconjugation on the redox properties of two model heme proteins, that is, cytochrome c (CytC) and myoglobin (Mb). In particular, the work focuses on the sequential modification by 3-dimethylamino propylamine (DMAPA) and 4-nonylphenyl 3-sulfopropyl ether (S1) anionic surfactant. Bioconjugation with DMAPA and S1 are the initial steps in the generation of pure liquid proteins, which remain active in the absence of water and up to temperatures above 150 °C. Thin-layer spectroelectrochemistry reveals that DMAPA cationization leads to a distribution of bioconjugate structures featuring reduction potentials shifted up to 380 mV more negative than the native proteins. Analysis based on circular dichroism, MALDI-TOF mass spectrometry, and zeta potential measurements suggest that the shift in the reduction potentials are not linked to protein denaturation, but to changes in the spin state of the heme. These alterations of the spin states originate from subtle structural changes induced by DMAPA attachment. Interestingly, electrostatic coupling of anionic surfactant S1 shifts the reduction potential closer to that of the native protein, demonstrating that the modifications of the heme electronic configuration are linked to surface charges.
- Published
- 2016
- Full Text
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11. 3D Bioprinting Using a Templated Porous Bioink.
- Author
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Armstrong JP, Burke M, Carter BM, Davis SA, and Perriman AW
- Subjects
- Adult Stem Cells cytology, Bone and Bones cytology, Cartilage cytology, Humans, Adult Stem Cells metabolism, Bone and Bones metabolism, Cartilage metabolism, Printing, Three-Dimensional, Tissue Engineering methods
- Abstract
3D tissue printing with adult stem cells is reported. A novel cell-containing multicomponent bioink is used in a two-step 3D printing process to engineer bone and cartilage architectures., (© 2016 The Authors. Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
- Published
- 2016
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12. Strategies for cell membrane functionalization.
- Author
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Armstrong JP and Perriman AW
- Subjects
- Animals, Humans, Biocompatible Materials metabolism, Cell Membrane chemistry, Cell Membrane physiology, Cell- and Tissue-Based Therapy methods
- Abstract
The ability to rationally manipulate and augment the cytoplasmic membrane can be used to overcome many of the challenges faced by conventional cellular therapies and provide innovative opportunities when combined with new biotechnologies. The focus of this review is on emerging strategies used in cell functionalization, highlighting both pioneering approaches and recent developments. These will be discussed within the context of future directions in this rapidly evolving field., (© 2016 by the Society for Experimental Biology and Medicine.)
- Published
- 2016
- Full Text
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13. Ultra-fast stem cell labelling using cationised magnetoferritin.
- Author
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Correia Carreira S, Armstrong JP, Seddon AM, Perriman AW, Hartley-Davies R, and Schwarzacher W
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- Female, Humans, Male, Mesenchymal Stem Cells metabolism, Apoferritins chemistry, Iron chemistry, Magnetite Nanoparticles chemistry, Materials Testing, Mesenchymal Stem Cells cytology, Oxides chemistry, Staining and Labeling methods
- Abstract
Magnetic cell labelling with superparamagnetic iron oxide nanoparticles (SPIONs) facilitates many important biotechnological applications, such as cell imaging and remote manipulation. However, to achieve adequate cellular loading of SPIONs, long incubation times (24 hours and more) or laborious surface functionalisation are often employed, which can adversely affect cell function. Here, we demonstrate that chemical cationisation of magnetoferritin produces a highly membrane-active nanoparticle that can magnetise human mesenchymal stem cells (hMSCs) using incubation times as short as one minute. Magnetisation persisted for several weeks in culture and provided significant T2* contrast enhancement during magnetic resonance imaging. Exposure to cationised magnetoferritin did not adversely affect the membrane integrity, proliferation and multi-lineage differentiation capacity of hMSCs, which provides the first detailed evidence for the biocompatibility of magnetoferritin. The combination of synthetic ease and flexibility, the rapidity of labelling and absence of cytotoxicity make this novel nanoparticle system an easily accessible and versatile platform for a range of cell-based therapies in regenerative medicine.
- Published
- 2016
- Full Text
- View/download PDF
14. Vaporisation and thermal decomposition of dialkylimidazolium halide ion ionic liquids.
- Author
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Lovelock KR, Armstrong JP, Licence P, and Jones RG
- Abstract
Vaporisation and liquid phase thermal decomposition, TD, of two halide ion ionic liquids, 1-octyl-3-methylimidazolium chloride, [C8C1Im]Cl, and 1-octyl-3-methylimidazolium iodide, [C8C1Im]I, are investigated using temperature programmed desorption (TPD) line of sight mass spectrometry (LOSMS) at ultra-high vacuum (UHV). The ability to use MS to distinguish between vaporisation and TD allows the thermodynamics/kinetics of both vaporisation and TD to be investigated within the same experiments. Vaporisation of both halide ion ionic liquids is demonstrated. For both [C8C1Im]Cl and [C8C1Im]I the vapour is shown to be composed of neutral ion pairs (NIPs). The enthalpy of vaporisation at temperature T, ΔvapHT, was experimentally determined as ΔvapH455 = 151 ± 10 kJ mol(-1) for [C8C1Im]Cl and ΔvapH480 = 149 ± 8 kJ mol(-1) for [C8C1Im]I. Extrapolation of ΔvapHT to the reference temperature, 298 K, gave ΔvapH298 = 166 ± 10 kJ mol(-1) for [C8C1Im]Cl and ΔvapH298 = 167 ± 8 kJ mol(-1) for [C8C1Im]I, higher than most ΔvapH298 values measured to date for other [C8C1Im](+)-containing ionic liquids. In addition, predictions of ΔvapH298 for other halide ion ionic liquids are made. Liquid phase TD is shown to proceed via nucleophilic substitution to give two sets of products: 1-octylimidazole and methylhalide, and 1-methylimidazole and 1-octylhalide. The activation energy of TD at a temperature T, Ea,TD,T, is measured for the nucleophilic substitution of [C8C1Im]I to give methyliodide; Ea,TD,480 = 136 ± 15 kJ mol(-1). Ea,TD,T is measured for the nucleophilic substitution of [C8C1Im]Cl to give methylchloride; Ea,TD,455 = 132 ± 10 kJ mol(-1). The fact that ΔvapHT and Ea,TD,T are the same (within error) for both ionic liquids is commented upon, and conclusions are drawn as to the thermal stability of these ionic liquids.
- Published
- 2014
- Full Text
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15. Mode-converters for rectangular-core fiber amplifiers to achieve diffraction-limited power scaling.
- Author
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Sridharan AK, Pax PH, Heebner JE, Drachenberg DR, Armstrong JP, and Dawson JW
- Subjects
- Computer-Aided Design, Equipment Design, Equipment Failure Analysis, Amplifiers, Electronic, Fiber Optic Technology instrumentation, Lasers, Refractometry instrumentation
- Abstract
A rectangular-core (ribbon) fiber that guides and amplifies a single higher-order-mode (HOM) can potentially scale to much higher average powers than what is possible in traditional circular-core large-mode-area fibers. Such an amplifier would require mode-conversion at the input to enable interfacing with seed sources that typically output TEM(00) mode radiation and at the output to generate diffraction-limited radiation for end-user applications. We present the first simulation and experimental results of a mode conversion technique that uses two diffractive-optic-elements in conjugate Fourier planes to convert a diffraction limited TEM(00) mode to the HOM of a ribbon fiber. Mode-conversion-efficiency is approximately 84% and can theoretically approach 100%. We also demonstrate a mode-converter system that converts a single HOM of a ribbon fiber back to a diffraction-limited TEM(00) mode. Conversion efficiency is a record 80.5%.
- Published
- 2012
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16. Magnetizing DNA and proteins using responsive surfactants.
- Author
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Brown P, Khan AM, Armstrong JP, Perriman AW, Butts CP, and Eastoe J
- Subjects
- Circular Dichroism, Hexanols chemistry, Magnetic Fields, Myoglobin chemistry, Proteins metabolism, Spectrophotometry, Ultraviolet, Trityl Compounds chemistry, DNA chemistry, Proteins chemistry, Surface-Active Agents chemistry
- Abstract
DNA chains and their movement in solvent may now be controlled simply by surfactant binding and the switching "on" and "off" of a magnetic field adding a new paradigm to the study and control, condensation and manipulation of DNA (and other biomolecules). Such control is essential for biotechnological applications such as transfection and the regulation of gene suppression, as well as in materials science concerning soft molecular self-assemblies., (Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
- Published
- 2012
- Full Text
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17. Vapourisation of ionic liquids.
- Author
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Armstrong JP, Hurst C, Jones RG, Licence P, Lovelock KR, Satterley CJ, and Villar-Garcia IJ
- Subjects
- Gases, Hot Temperature, Mass Spectrometry, Static Electricity, Volatilization, Imidazoles chemistry, Ionic Liquids chemistry
- Abstract
Eight common imidazolium based ionic liquids have been successfully evaporated in ultra-high vacuum, their vapours analysed by line of sight mass spectrometry and their heats (enthalpy) of vapourisation determined. They were found to evaporate as ion pairs, with heats of vapourisation which depend primarily on the coulombic interactions within the liquid phase and the gas phase ion pair. An electrostatic model is presented relating the heats of vapourisation to the molar volumes of the ionic liquids.
- Published
- 2007
- Full Text
- View/download PDF
18. A new method for identifying the depth of insertion of tracheal tubes.
- Author
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Goldman JM, Armstrong JP, Vaught LE, and Daniel LC
- Subjects
- Adult, Female, Humans, Intubation, Intratracheal instrumentation, Magnetics, Male, Palpation, Trachea anatomy & histology, Intubation, Intratracheal methods
- Abstract
When a patient's lungs require mechanical ventilation, a plastic breathing tube (tracheal tube, TT) must be inserted through the vocal cords and into the trachea. Although insertion of the TT is a routine procedure, determining the correct depth of insertion is difficult clinically, and may require a chest radiograph. We investigated the accuracy of a simple clinical method for inserting a TT to the correct depth. Typically, an inflatable cuff surrounds the distal circumference of the TT to occlude the gap between TT and tracheal wall. Our technique involves pressing gently with one finger on the front of the lower part of the neck to locate the center of the cuff. We hypothesized that a) a palpating finger can identify the cuff through the tracheal wall, and b) if placed using this technique, the TT will be correctly positioned in the trachea. We studied 79 patients in whom TTs were inserted for general anesthesia. TTs were inserted to a depth determined by the palpation technique. We developed a stylet incorporating a magnetic reed switch which could be inserted through the TT. A magnet with specific field characteristics was passed along the front of the neck to locate the reed switch and confirm the location of the cuff. The TT position in the trachea was then examined with a fiberoptic scope. The palpation technique resulted in optimal positioning of the TT in all subjects and could save approximately $14,268/year in chest radiograph costs in our institution.
- Published
- 1995
19. Rosette formation between human lymphocytes and sheep erythrocytes. Inhibition of rosette formation by specific glycopeptides.
- Author
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Boldt DH and Armstrong JP
- Subjects
- Animals, Binding, Competitive, Guinea Pigs, Humans, Lymphocytes metabolism, Oligosaccharides pharmacology, Sheep, Spleen cytology, Spleen immunology, Trypsin, alpha-Fetoproteins metabolism, Erythrocytes immunology, Glycopeptides pharmacology, Immune Adherence Reaction, Lymphocytes immunology
- Abstract
Rosette formation with unsensitized sheep erythrocytes is a characteristic of human thymus dependent lymphocytes. Release of glycopeptides from the sheep erythrocyte by trypsin reduces rosette formation. These tryptic glycopeptides inhibit rosette formation by untrypsinized sheep erythrocytes; this suggests that rosetting is mediated by erythrocyte surface glycopeptides. To investigate the molecular nature of this interaction, we examined the abilities of various model compounds to act as haptenic inhibitors of rosette formation. Inhibition is given by glycopeptides bearing oligosaccharide units rich in sialic acid, galactose, N-acetylglucosamine, and mannose linked to asparagine residues through glycosylamine bonds. Among compounds tested, fetuin glycopeptide is most effective, but human transferrin glycopeptide and human erythrocyte glycopeptide I also inhibit rosette formation. Other compounds including human erythrocyte glycopeptide II, human IgG glycopeptide, lacto-N-neotetraose, 3'- and 6'-sialyllactose show no significant inhibition. Neither sialic acid, galactose, manose, nor N-acetyl-glucosamine alone inhibits rosette formation. Stepwise degradation of fetuin glycopeptide established the galactose residues as important determinants of inhibitory activity. Fetuin glycopeptide blocks rosette formation when added to a suspension of human lymphocytes and sheep erythrocytes or when preincubated with human lymphocytes, but not when preincubated with sheep erythrocytes. Studies of the binding of [3H] fetuin glycopeptide to normal lymphocytes demonstrate 7.5 x 10(6) saturable binding sites per cell. No saturable binding of this compound to sheep erythrocyte membranes is observed. Compared to normals, lymphocytes from patients with chronic lymphatic leukemia demonstrate decreased fetuin glycopeptide binding with a mean of 0.9 x 10(6) sites per cell. This decreased binding correlates with the impaired ability of these cells to form rosettes. The data suggest that fetuin glycopeptide inhibits rosette formation by binding to the thymus-dependent cell where competition occurs with sheep erythrocytes for specific lymphocyte surface receptors.
- Published
- 1976
- Full Text
- View/download PDF
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