143 results on '"Arias, JM"'
Search Results
2. When a tooth extraction unmasks the malignancy within: an unusual case of squamous cell carcinoma of the mandible
- Author
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Rivera, A Gonzalez, primary, Arias, JM Bretón, additional, Puig, JM Hernandez, additional, Roman, L Ortiz, additional, and Rivera, J Nieves, additional
- Published
- 2024
- Full Text
- View/download PDF
3. 292 - When a tooth extraction unmasks the malignancy within: an unusual case of squamous cell carcinoma of the mandible
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Rivera, A Gonzalez, Arias, JM Bretón, Puig, JM Hernandez, Roman, L Ortiz, and Rivera, J Nieves
- Published
- 2024
- Full Text
- View/download PDF
4. 186 - The other side of hypoactivity: recognizing myxedema coma in a geriatric patient
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Arias, JM Bretón, Mejias, C Plaza, Rivera, A González, and Colon, J
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- 2024
- Full Text
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5. An extended Agassi model: algebraic structure, phase diagram, and large size limit
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García-Ramos, J E, primary, Dukelsky, J, additional, Pérez-Fernández, P, additional, and Arias, JM, additional
- Published
- 2019
- Full Text
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6. Shape phase transition in odd nuclei in a multi-j model: The U-B(6)circle times U-F(12) case
- Author
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Alonso, Ce, Arias, Jm, and Vitturi, Andrea
- Published
- 2007
7. U(5)-O(6) transition in the interacting boson model and the E(5) critical point symmetry
- Author
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Arias, Jm, Alonso, Ce, and Vitturi, Andrea
- Published
- 2003
8. Four-body continuum-discretized coupled-channels calculations: Application to 6He+64Zn at 13.6 MeV
- Author
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Rodríguez-Gallardo, M, Arias, JM, Gómez-Camacho, J, Moro, AM, Thompson, J, Tostevin, JA, Rodríguez-Gallardo, M, Arias, JM, Gómez-Camacho, J, Moro, AM, Thompson, J, and Tostevin, JA
- Abstract
The recently developed four-body continuum-discretized coupled-channels (CDCC) method, making use of the binning procedure [1], is applied to the reaction 6He+64Zn at 13.6 MeV (around the Coulomb barrier). Excellent agreement with available elastic data [2] is found.
- Published
- 2010
9. Four-body continuum-discretized coupled-channels calculations
- Author
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Rodriguez-Gallardo, M, Arias, JM, Gomez-Camacho, J, Moro, AM, Thompson, IJ, Tostevin, JA, Rodriguez-Gallardo, M, Arias, JM, Gomez-Camacho, J, Moro, AM, Thompson, IJ, and Tostevin, JA
- Published
- 2009
10. Four-body continuum-discretized coupled-channels calculations using a transformed harmonic oscillator basis
- Author
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Rodriguez-Gallardo, M, Arias, JM, Gomez-Camacho, J, Johnson, RC, Moro, AM, Thompson, IJ, Tostevin, JA, Rodriguez-Gallardo, M, Arias, JM, Gomez-Camacho, J, Johnson, RC, Moro, AM, Thompson, IJ, and Tostevin, JA
- Published
- 2008
11. Continuum effects: Structure and reactions of He-6
- Author
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Rodriguez-Gallardo, M, Arias, JM, Gomez-Camacho, J, Johnson, RC, Moro, AM, Thompson, IJ, Tostevin, JA, Rodriguez-Gallardo, M, Arias, JM, Gomez-Camacho, J, Johnson, RC, Moro, AM, Thompson, IJ, and Tostevin, JA
- Published
- 2007
12. A general algebraic model for vibrational molecular spectroscopy
- Author
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Frank, A., Lemus, R., Perez-Bernal, F., Roelof Bijker, and Arias, Jm
- Subjects
Chemical Physics (physics.chem-ph) ,Physics - Chemical Physics ,FOS: Physical sciences - Abstract
We present a symmetry-adapted version of the vibron model and discuss an application to D_{3h} triatomic molecules: H_3^+, Be_3 and Na_3^+., Comment: 3 pages, invited talk at `21st International Colloquium on Group Theoretical Methods in Physics', Goslar, Germany, July 15-20, 1996
- Published
- 1996
13. A local anharmonic treatment of vibrations of methane
- Author
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Lemus, R., Frank, A., Roelof Bijker, Perez-Bernal, F., Arias, Jm, and Universidad de Sevilla. Departamento de Física Atómica, Molecular y Nuclear
- Subjects
Chemical Physics (physics.chem-ph) ,Physics - Chemical Physics ,FOS: Physical sciences ,Astrophysics::Earth and Planetary Astrophysics ,Physics::Chemical Physics - Abstract
The stretching and bending vibrations of methane are studied in a local anharmonic model of molecular vibrations. The use of symmetry-adapted operators reduces the eigenvalue problem to block diagonal form. For the 44 observed energies we obtain a fit with a standard deviation of 0.81 cm$^{-1}$ (and a r.m.s. deviation of 1.16 cm$^{-1}$)., revised and updated version: 13 pages, 3 tables, Revista Mexicana de Fisica, in press
- Published
- 1996
14. Three-body continuum discretization in a basis of transformed harmonic oscillator states
- Author
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Rodriguez-Gallardo, M, Arias, JM, Gomez-Camacho, J, Moro, AM, Thompson, IJ, Tostevin, JA, Rodriguez-Gallardo, M, Arias, JM, Gomez-Camacho, J, Moro, AM, Thompson, IJ, and Tostevin, JA
- Published
- 2005
15. Low-rate wireless personal area networks applied to street lighting
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Domingo-Perez, F, primary, Gil-de-Castro, A, additional, Flores-Arias, JM, additional, Bellido-Outeirino, FJ, additional, and Moreno-Munoz, A, additional
- Published
- 2011
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16. Low-rate wireless personal area networks applied to street lighting.
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Domingo-Perez, F, Gil-de-Castro, A, Flores-Arias, JM, Bellido-Outeirino, FJ, and Moreno-Munoz, A
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WIRELESS personal area networks ,STREET lighting ,ENERGY consumption ,REMOTE access networks ,WIRELESS communications ,BALLASTS (Electricity) ,ELECTRIC lighting - Abstract
This paper focuses on the development of an energy-efficient street lighting remote management system making use of low-rate wireless personal area networks and the digital addressable lighting interface (DALI) protocol to get the bidirectional communication necessary for checking lamp parameters like lamp status, current level, etc. Because of the fact that two-thirds of the installed street lighting systems use old and inefficient technologies, there exists a huge potential to renew street lighting and save energy consumption. The proposed system uses the DALI protocol in street lighting, increasing the maximum number of ballasts that can be controlled with DALI – originally it can only drive up to 64 ballasts. Some aspects of the wireless communication system and experimental measurements are presented and discussed. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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17. Transforming growth factor-ß1 gene polymorphisms are associated with disease progression in idiopathic pulmonary fibrosis.
- Author
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Xaubet A, Marin-Arguedas A, Lario S, Ancochea J, Morell F, Ruiz-Manzano J, Rodriguez-Becerra E, Rodriguez-Arias JM, Iñigo P, Sanz S, Campistol JM, Mullol J, and Picado C
- Abstract
Transforming growth factor-beta1 (TGF-beta1) is a cytokine that plays a key role in the development of idiopathic pulmonary fibrosis. There have been reports on the presence of two genetic polymorphisms in the DNA sequence encoding the leader sequence of the TGF-beta1 protein, located in codons 10 and 25. The objective of this study was to investigate the association between TGF-beta1 gene polymorphisms in codons 10 and 25 and the susceptibility to idiopathic pulmonary fibrosis and the progression of the disease. Compared with healthy control subjects (n = 140), patients with idiopathic pulmonary fibrosis (n = 128) showed no significant deviations in genotype or allele frequencies. One hundred and ten patients with idiopathic pulmonary fibrosis were followed up for 30.3 +/- 25 months. The presence of a proline allele at codon 10 was independently associated with a significant increase in alveolar arterial oxygen tension difference during follow-up, after controlling for the effect of treatment (coefficient = 0.59; 95% confidence intervals, 0.23 to 0.96; p = 0.002). These findings suggest that (1) TGF-beta1 gene polymorphisms in codons 10 and 25 do not predispose to the development of idiopathic pulmonary fibrosis; and (2) TGF-beta1 gene polymorphisms may affect disease progression in patients with idiopathic pulmonary fibrosis. [ABSTRACT FROM AUTHOR]
- Published
- 2003
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18. Determination of glomerular filtration rate using technetium-99m-DTPA with differing degrees of renal function.
- Author
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Morton KA, Pisani DE, Whiting JH Jr., Cheung AK, Arias JM, Valdivia S, Morton, K A, Pisani, D E, Whiting, J H Jr, Cheung, A K, Arias, J M, and Valdivia, S
- Published
- 1997
19. A symmetry adapted approach to molecular spectroscopy: The anharmonic oscillator symmetry model
- Author
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Frank, A., Lemus, R., Roelof Bijker, Perezbernal, F., Arias, Jm, and Universidad de Sevilla. Departamento de Física Atómica, Molecular y Nuclear
- Subjects
Chemical Physics (physics.chem-ph) ,Physics - Chemical Physics ,FOS: Physical sciences ,Physics::Chemical Physics - Abstract
We apply the Anharmonic Oscillator Symmetry Model to the description of vibrational excitations in ${\cal D}_{3h}$ and ${\cal T}_d$ molecules. A systematic procedure can be used to establish the relation between the algebraic and configuration space formulations, by means of which new interactions are found in the algebraic model, leading to reliable spectroscopic predictions. We illustrate the method for the case of ${\cal D}_{3h}$-triatomic molecules and the ${\cal T}_d$ Be-cluster., Comment: 12 pages, invited talk at XIX Oaxtepec Symposium on Nuclear Physics, January 1996
20. A microscopic theory of magnetic order in strongly correlated quantum spin lattices
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Bishop, RF, Farnell, DJJ, Arias, JM, Gallardo, MI, and Lozano, M
- Abstract
Quantum spins arranged on regular geometrical lattices, and interacting through a wide diversity of specified model Hamiltonians, provide a rich variety of magnetically ordered phases with many subtle properties. The zero-temperature quantum phase transitions between states of different order can be explored by the variation of internal control parameters within the interaction Hamiltonian. We illustrate how the systematic inclusion of multi-spin correlations for such lattice systems may be efficiently and effectively implemented via the coupled cluster method (CCM), which has been widely demonstrated on many previous occasions to be one of the most versatile methods of microscopic quantum many-body theory. Its power is illustrated by application to the spin-½ anisotropic Heisenberg (or XXZ) model on an infinite square lattice. The results obtained for such properties as the ground-state energy and sublattice magnetization are found to be as accurate as those obtained by any available alternative method. We show also how the CCM can now provide accurate ab initio estimates for the critical values of the parameters which mark the positions of phase boundaries between states of different quantum order, as well as for the critical indices associated with the singular behaviour near these transition points of various physical quantities.
- Published
- 1998
21. A microscopic theory of magnetic order in strongly correlated quantum spin lattices
- Author
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Bishop, RF and Farnell, DJJ, Arias, JM, and Gallardo, MI and Lozano, M
- Published
- 1998
22. Molecular cloning, functional characterization and differential expression of two novel GABA A R-like subunits from red swamp crayfish Procambarus clarkii.
- Author
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Valladares-Hernández IU, Hernández-Martínez JM, Cuaxospa JM, Jiménez-Vázquez EN, Sánchez-Jaramillo E, Arias JM, and García U
- Abstract
In this work, we cloned and functionally expressed two novel GABA
A receptor subunits from Procambarus clarkii crayfish. These two new subunits, PcGABAA -α and PcGABAA -β2, revealed significant sequence homology with the PcGABAA -β subunit, previously identified in our laboratory. In addition, PcGABAA -α subunit also shared a significant degree of identity with the Drosophila melanogaster genes DmGRD (GABA and glycine-like receptor subunits of Drosophila) as well as PcGABAA -β2 subunit with DmLCCH3 (ligand-gated chloride channel homolog 3). Electrophysiological recordings showed that the expression in HEK cells of the novel subunits, either alone or in combination, failed to form functional homo- or heteromeric receptors. However, the co-expression of PcGABAA -α with PcGABAA -β evoked sodium- or chloride-dependent currents that accurately reproduced the time course of the GABA-evoked currents in the X-organ neurons from crayfish, suggesting that these GABA subunits combine to form two types of GABA receptors, one with cationic selectivity filter and the other preferentially permeates anions. On the other hand, PcGABAA -β2 and PcGABAA -β co-expression generated a chloride current that does not show desensitization. Muscimol reproduced the time course of GABA-evoked currents in all functional receptors, and picrotoxin blocked these currents; bicuculline did not block any of the recorded currents. Reverse transcription polymerae chain reaction (RT-PCR) amplifications and FISH revealed that PcGABAA -α and PcGABAA -β2 are predominantly expressed in the crayfish nervous system. Altogether, these findings provide the first evidence of a neural GABA-gated cationic channel in the crayfish, increasing our understanding of the role of these new GABAA receptor subunits in native heteromeric receptors., (© 2024 Federation of European Neuroscience Societies and John Wiley & Sons Ltd.)- Published
- 2024
- Full Text
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23. Identifying functional cortical plasticity after spinal tumour resection using navigated transcranial magnetic stimulation.
- Author
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Onyiriuka L, Aliaga-Arias JM, Patel S, Khan A, Ashkan K, Gullan R, Bhangoo R, Ahmed A, Grahovac G, Vergani F, Kailaya-Vasan A, and Lavrador JP
- Abstract
Our aim was to investigate the effectiveness of navigated transcranial magnetic stimulation (nTMS) brain mapping to characterise preoperative motor impairment caused by an intradural extramedullary (IDEM) tumour and postoperative cortical functional reorganisation. Preoperative and 1-year follow-up clinical, radiological and nTMS data from a case of thoracic spinal meningioma that underwent surgical resection of the lesion were collected and compared. A 67-year-old patient presented with severe progressive thoracic myelopathy (hypertonic paraparesis, clonus, insensate urinary retention) secondary to an IDEM tumour. Initial nTMS assessment showed bilateral upper limb representation with no positive responses for both lower limbs. He underwent successful surgical resection for his IDEM (meningioma WHO grade 1). At 1-year follow-up, the patient's gait was improved and his bladder function normalised. nTMS documented positive responses for both upper and lower limbs and a decrease in the area (right side: 1.01 vs 0.39cm
2 ; left side: 1.92 vs 0.81cm2 ) and volume (right side: 344.2 vs 42.4uVcm2 ; left side: 467.1 vs 119uVcm2 ) of cortical activation for both upper limbs, suggesting a functional reorganisation of the motor areas after tumour resection. nTMS motor mapping and derived metrics can characterise preoperative motor deficit and cortical plasticity during follow-up after IDEM resection.- Published
- 2024
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24. Application of Microfracture Analysis to Fatigue Fractures in Materials through Non-Destructive Tests.
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Sánchez-Santana U, Presbítero-Espinosa G, and Quiroga-Arias JM
- Abstract
Fatigue fractures in materials are the main cause of approximately 80% of all material failures, and it is believed that such failures can be predicted and mathematically calculated in a reliable manner. It is possible to establish prediction modalities in cases of fatigue fractures according to three fundamental variables in fatigue, such as volume, number of fracture cycles, as well as applied stress, with the integration of Weibull constants (length characteristic). In this investigation, mechanical fatigue tests were carried out on specimens smaller than 4 mm
2 , made of different industrial materials. Their subsequent analysis was performed through precision computed tomography, in search for microfractures. The measurement of these microfractures, along with their metrics and classifications, was recorded. A convolutional neural network trained with deep learning was used to achieve the detection of microfractures in image processing. The detection of microfractures in images with resolutions of 480 × 854 or 960 × 960 pixels is the primary objective of this network, and its accuracy is above 95%. Images that have microfractures and those without are classified using the network. Subsequently, by means of image processing, the microfracture is isolated. Finally, the images containing this feature are interpreted using image processing to obtain their area, perimeter, characteristic length, circularity, orientation, and microfracture-type metrics. All values are obtained in pixels and converted to metric units (μm) through a conversion factor based on image resolution. The growth of microfractures will be used to define trends in the development of fatigue fractures through the studies presented.- Published
- 2024
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25. Decrease in the intensive care unit-acquired weakness with a multicomponent protocol implementation: A quasi-experimental clinical trial
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Giraldo ND, Carvajal C, Muñoz F, Restrepo MP, García MA, Arias JM, Mojica JL, Torres JC, García Á, Muñoz D, Rodríguez FC, Arias J, Mejía LM, and De La Rosa G
- Subjects
- Humans, Hospitals, Pain
- Abstract
Introduction: Intensive care unit-acquired weakness is a frequent complication that affects the prognosis of critical illness during hospital stay and after hospital discharge., Objectives: To determine if a multicomponent protocol of early active mobility involving adequate pain control, non-sedation, non-pharmacologic delirium prevention, cognitive stimulation, and family support, reduces intensive care unit-acquired weakness at the moment of discharge., Materials and Methods: We carried out a non-randomized clinical trial in two mixed intensive care units in a high-complexity hospital, including patients over 14 years old with invasive mechanical ventilation for more than 48 hours. We compared the intervention –the multicomponent protocol– during intensive care hospitalization versus the standard care., Results: We analyzed 82 patients in the intervention group and 106 in the control group. Muscle weakness acquired in the intensive care unit at the moment of discharge was less frequent in the intervention group (41.3% versus 78.9%, p<0.00001). The mobility score at intensive unit care discharge was better in the intervention group (median = 3.5 versus 2, p < 0.0138). There were no statistically significant differences in the invasive mechanical ventilation-free days at day 28 (18 versus 15 days, p<0.49), and neither in the mortality (18.2 versus 27.3%, p<0.167)., Conclusion: A multi-component protocol of early active mobility significantly reduces intensive care unit-acquired muscle weakness at the moment of discharge.
- Published
- 2023
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26. Lack of apoptosis leads to cellular senescence and tumorigenesis in Drosophila epithelial cells.
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Garcia-Arias JM, Pinal N, Cristobal-Vargas S, Estella C, and Morata G
- Abstract
Programmed cell death (apoptosis) is a homeostasis program of animal tissues designed to remove cells that are unwanted or are damaged by physiological insults. To assess the functional role of apoptosis, we have studied the consequences of subjecting Drosophila epithelial cells defective in apoptosis to stress or genetic perturbations that normally cause massive cell death. We find that many of those cells acquire persistent activity of the JNK pathway, which drives them into senescent status, characterized by arrest of cell division, cell hypertrophy, Senescent Associated ß-gal activity (SA-ß-gal), reactive oxygen species (ROS) production, Senescent Associated Secretory Phenotype (SASP) and migratory behaviour. We have identified two classes of senescent cells in the wing disc: 1) those that localize to the appendage part of the disc, express the upd, wg and dpp signalling genes and generate tumour overgrowths, and 2) those located in the thoracic region do not express wg and dpp nor they induce tumour overgrowths. Whether to become tumorigenic or non-tumorigenic depends on the original identity of the cell prior to the transformation. We also find that the p53 gene contributes to senescence by enhancing the activity of JNK., (© 2023. The Author(s).)
- Published
- 2023
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27. Excited-state quantum phase transitions in the anharmonic Lipkin-Meshkov-Glick model: Dynamical aspects.
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Khalouf-Rivera J, Gamito J, Pérez-Bernal F, Arias JM, and Pérez-Fernández P
- Abstract
The standard Lipkin-Meshkov-Glick (LMG) model undergoes a second-order ground-state quantum phase transition (QPT) and an excited-state quantum phase transition (ESQPT). The inclusion of an anharmonic term in the LMG Hamiltonian gives rise to a second ESQPT that alters the static properties of the model [Gamito et al., Phys. Rev. E 106, 044125 (2022)2470-004510.1103/PhysRevE.106.044125]. In the present work, the dynamical implications associated to this new ESQPT are analyzed. For that purpose, a quantum quench protocol is defined on the system Hamiltonian that takes an initial state, usually the ground state, into a complex excited state that evolves on time. The impact of the new ESQPT on the time evolution of the survival probability and the local density of states after the quantum quench, as well as on the Loschmidt echoes and the microcanonical out-of-time-order correlator (OTOC) are discussed. The anharmonity-induced ESQPT, despite having a different physical origin, has dynamical consequences similar to those observed in the ESQPT already present in the standard LMG model.
- Published
- 2023
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28. Heterogeneity of the endoplasmic reticulum Ca 2+ store determines colocalization with mitochondria.
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Sánchez-Vázquez VH, Martínez-Martínez E, Gallegos-Gómez ML, Arias JM, Pallafacchina G, Rizzuto R, and Guerrero-Hernández A
- Subjects
- Humans, HeLa Cells, HEK293 Cells, Thapsigargin pharmacology, Calcium metabolism, Calcium Signaling, Endoplasmic Reticulum metabolism, Mitochondria metabolism
- Abstract
Contact sites between the endoplasmic reticulum (ER) and mitochondria play a pivotal role in cell signaling, and the interaction between these organelles is dynamic and finely regulated. We have studied the role of ER Ca
2+ concentration ([Ca2+ ]ER ) in modulating this association in HeLa and HEK293 cells and human fibroblasts. According to Manders' coefficient, ER-mitochondria colocalization varied depending on the ER marker; it was the highest with ER-Tracker and the lowest with ER Ca2+ indicators (Mag-Fluo-4, erGAP3, and G-CEPIA1er) in both HeLa cells and human fibroblasts. Only GEM-CEPIA1er displayed a high colocalization with elongated mitochondria in HeLa cells, this ER Ca2+ indicator reveals low Ca2+ regions because this ion quenches its fluorescence. On the contrary, the typical rounded and fragmented mitochondria of HEK293 cells colocalized with Mag-Fluo-4 and, to a lesser extent, with GEM-CEPIA1er. The ablation of the three IP3 R isoforms in HEK293 cells increased mitochondria-GEM-CEPIA1er colocalization. This pattern of colocalization was inversely correlated with the rate of ER Ca2+ leak evoked by thapsigargin (Tg). Moreover, Tg and Histamine in the absence of external Ca2+ increased mitochondria-ER colocalization. On the contrary, in the presence of external Ca2+ , both Bafilomycin A1 and Tg reduced the mitochondria-ER interaction. Notably, knocking down MCU decreased mitochondria-ER colocalization. Overall, our data suggest that the [Ca2+ ] is not homogenous within the ER lumen and that mitochondria-ER interaction is modulated by the ER Ca2+ leak and the [Ca2+ ]i ., Competing Interests: Declaration of Competing Interest The authors declare no competing financial interests., (Copyright © 2022. Published by Elsevier Ltd.)- Published
- 2023
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29. Complete Genome Sequences of Actinobacteriophages Anaysia and Caviar.
- Author
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Abidin ZU, Aucapina JE, Beauzil S, Berotte CM, Bonsu AO, Burgos GY, Chak STC, Collymore A, Daley ER, Defarias R, Ghobrial V, Gill SS, Huertas-Arias JM, Joseph H, Kaur N, Khan U, Klein CJ, Lazo H, Li Y, Miller OB, Muñoz JJ, Nieto-Fernandez FE, Nisbett LM, Owens D, Patel SM, Paulino EJ, Pender S, Perkins SM, Persaud A, Pierrot T, Raja I, Riley KL, Romero S, Sarmiento PG, Shorter K, Smith S, Tahir W, and Ukekwe CA
- Abstract
Anaysia and Caviar are temperate siphoviruses isolated from soil using Gordonia terrae 3612 and Mycobacterium smegmatis mc
2 155, respectively. Anaysia's 52,861-bp genome carries 102 genes, while Caviar's 47,074-bp genome carries 79 genes. Based on gene content similarity, Anaysia and Caviar are assigned to phage clusters A15 and A3, respectively.- Published
- 2022
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30. Sutimlimab improves quality of life in patients with cold agglutinin disease: results of patient-reported outcomes from the CARDINAL study.
- Author
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Röth A, Barcellini W, Tvedt THA, Miyakawa Y, Kuter DJ, Su J, Jiang X, Hobbs W, Arias JM, Shafer F, and Weitz IC
- Subjects
- Antibodies, Monoclonal, Humanized, Fatigue etiology, Humans, Patient Reported Outcome Measures, Treatment Outcome, Anemia, Hemolytic, Autoimmune drug therapy, Quality of Life
- Abstract
Patients with cold agglutinin disease (CAD) experience fatigue and poor quality of life. However, previous CAD-related studies have not explored patient-reported outcomes such as the Functional Assessment of Chronic Illness Therapy (FACIT)-Fatigue. Sutimlimab, a C1s complement inhibitor, has been shown to halt haemolysis in CAD. Here, we present 26-weeks' patient-reported data from CARDINAL Part A (ClinicalTrials.gov, NCT03347396), which assessed efficacy and safety of sutimlimab in patients with CAD and recent history of transfusion. Aside from measuring changes in haemolytic markers, FACIT-Fatigue was measured at the treatment assessment timepoint (TAT; average of weeks 23, 25, and 26). Exploratory endpoints included the change in EuroQol 5-dimension 5-level questionnaire (EQ-5D-5L) and the 12-Item Short Form Health Survey (SF-12) at TAT, and Patient Global Impression of Change (PGIC), and Patient Global Impression of (fatigue) Severity (PGIS) at week 26. Mean (range) FACIT-Fatigue scores increased from 32.5 (14.0-47.0) at baseline (a score indicative of severe fatigue) to 44.3 (28.0-51.0) at TAT. Considerable improvements were reported for EQ-5D-5L at TAT, SF-12 scores at TAT, and PGIC and PGIS scores at week 26. Sutimlimab treatment resulted in sustained improvements in symptoms of fatigue and overall quality of life in patients with CAD. NCT03347396. Registered 20 November, 2017., (© 2022. The Author(s).)
- Published
- 2022
- Full Text
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31. Excited-state quantum phase transitions in the anharmonic Lipkin-Meshkov-Glick model: Static aspects.
- Author
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Gamito J, Khalouf-Rivera J, Arias JM, Pérez-Fernández P, and Pérez-Bernal F
- Abstract
The basic Lipkin-Meshkov-Glick model displays a second-order ground-state quantum phase transition and an excited-state quantum phase transition (ESQPT). The inclusion of an anharmonic term in the Hamiltonian implies a second ESQPT of a different nature. We characterize this ESQPT using the mean field limit of the model. The alternative ESQPT, associated with the changes in the boundary of the finite Hilbert space of the system, can be properly described using the order parameter of the ground-state quantum phase transition, the energy gap between adjacent states, the participation ratio, and the quantum fidelity susceptibility.
- Published
- 2022
- Full Text
- View/download PDF
32. Healthcare and social needs of international migrants during the COVID-19 pandemic in Latin America: analysis of the Chilean case.
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Blukacz A, Cabieses B, Mezones-Holguín E, and Cardona Arias JM
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- Humans, Chile epidemiology, Pandemics, Latin America epidemiology, Delivery of Health Care, Transients and Migrants, COVID-19 epidemiology
- Abstract
International migrants are a particularly vulnerable group in the context of the coronavirus disease 2019 (COVID-19) pandemic. Immigrants in Chile tend to experience multidimensional poverty and layers of social vulnerability. Our analysis aims to describe the perceived social and health-related needs of international migrants during the COVID-19 pandemic in Chile in terms of migration as a social determinant of health and layered social vulnerability. We carried out a qualitative analysis of responses to an open-ended question focused on the social and health-related needs linked to the pandemic included in an online questionnaire disseminated during April 2020 aimed at international migrants residing in Chile. The information gathered was thematically analysed. We included 1690 participants. They expressed needs related to health and others linked to the overall socio-economic and political response, employment, material conditions and psychosocial aspects. They also reported needs related to 'being a migrant'. Additionally, some participants described situations of vulnerability. We analysed their needs and situations of vulnerability identified around the following emerging frames: (a) work and living conditions, (b) regularisation traps and perceived lack of support and (c) and physical and mental health needs. International migrants in Chile report experiencing interrelated layers of social vulnerability during the COVID-19 pandemic, where 'being a migrant' exacerbates physical and mental health risks. The issues revealed are immediate and direct public health challenges, as well as different aspects of social vulnerability linked to migratory status, employment and barriers to accessing healthcare that should be addressed through comprehensive policies and measures.
- Published
- 2022
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33. PKC-Mediated Orai1 Channel Phosphorylation Modulates Ca 2+ Signaling in HeLa Cells.
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Martínez-Martínez E, Sánchez-Vázquez VH, León-Aparicio D, Sanchez-Collado J, Gallegos-Gómez ML, Rosado JA, Arias JM, and Guerrero-Hernández A
- Subjects
- Adenosine Triphosphate metabolism, Adenosine Triphosphate pharmacology, HeLa Cells, Humans, ORAI1 Protein metabolism, Phosphorylation, Protein Kinase C metabolism, Thapsigargin pharmacology, Calcium metabolism, Calcium Channels metabolism
- Abstract
The overexpression of the Orai1 channel inhibits SOCE when using the Ca
2+ readdition protocol. However, we found that HeLa cells overexpressing the Orai1 channel displayed enhanced Ca2+ entry and a limited ER depletion in response to the combination of ATP and thapsigargin (TG) in the presence of external Ca2+ . As these effects require the combination of an agonist and TG, we decided to study whether the phosphorylation of Orai1 S27/S30 residues had any role using two different mutants: Orai1-S27/30A (O1-AA, phosphorylation-resistant) and Orai1-S27/30D (O1-DD, phosphomimetic). Both O1-wt and O1-AA supported enhanced Ca2+ entry, but this was not the case with O1-E106A (dead-pore mutant), O1-DD, and O1-AA-E106A, while O1-wt, O1-E106A, and O1-DD inhibited the ATP and TG-induced reduction of ER [Ca2+ ], suggesting that the phosphorylation of O1 S27/30 interferes with the IP3 R activity. O1-wt and O1-DD displayed an increased interaction with IP3 R in response to ATP and TG; however, the O1-AA channel decreased this interaction. The expression of mCherry-O1-AA increased the frequency of ATP-induced sinusoidal [Ca2+ ]i oscillations, while mCherry-O1-wt and mCherry-O1-DD decreased this frequency. These data suggest that the combination of ATP and TG stimulates Ca2+ entry, and the phosphorylation of Orai1 S27/30 residues by PKC reduces IP3 R-mediated Ca2+ release.- Published
- 2022
- Full Text
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34. Executive function deficit in bipolar offspring: A neurocognitive endophenotype?
- Author
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Valencia-Echeverry J, Cuartas-Arias JM, Vélez JI, Arcos-Burgos M, López-Jaramillo C, and Palacio-Ortiz JD
- Subjects
- Adolescent, Adult, Child, Cohort Studies, Endophenotypes, Humans, Neuropsychological Tests, Young Adult, Bipolar Disorder genetics, Executive Function
- Abstract
Background: Recent studies in bipolar offspring (BO) showed that a low cognitive performance, especially executive function deficit, could be an early marker of bipolar disorder (BD). Nevertheless, these findings have not been replicated (specifically attentional control, flexibility, and working memory). In addition, most studies have focused on children and adolescents, but few studies analyze the executive function performance in BO adults., Objective: Our goal was to compare the neurocognitive performance of BO with control parent-offspring (CO) in a sample that included various age groups., Method: We conducted a cohort study, including subjects between six to 30 years old. We evaluated 129 BO and 113 CO subjects using validated psychiatric diagnostic interviews and an extensive neuropsychological battery., Results: Compared to the CO group, the BO group presented a lower performance in several executive functioning domains, mainly in tasks of attentional control, flexibility, and working memory. All age groups exhibited these findings., Conclusions: BO group presents executive function deficits, regardless of the age group: children, adolescents, and adults. This neurocognitive deficit should be accountable as a neurocognitive endophenotype candidate in BD., (Copyright © 2021. Published by Elsevier B.V.)
- Published
- 2022
- Full Text
- View/download PDF
35. Entropies and IPR as Markers for a Phase Transition in a Two-Level Model for Atom-Diatomic Molecule Coexistence.
- Author
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Baena I, Pérez-Fernández P, Rodríguez-Gallardo M, and Arias JM
- Abstract
A quantum phase transition (QPT) in a simple model that describes the coexistence of atoms and diatomic molecules is studied. The model, which is briefly discussed, presents a second-order ground state phase transition in the thermodynamic (or large particle number) limit, changing from a molecular condensate in one phase to an equilibrium of diatomic molecules-atoms in coexistence in the other one. The usual markers for this phase transition are the ground state energy and the expected value of the number of atoms (alternatively, the number of molecules) in the ground state. In this work, other markers for the QPT, such as the inverse participation ratio (IPR), and particularly, the Rényi entropy, are analyzed and proposed as QPT markers. Both magnitudes present abrupt changes at the critical point of the QPT.
- Published
- 2022
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- View/download PDF
36. The Antioxidant Effect of Colombian Berry ( Vaccinium meridionale Sw.) Extracts to Prevent Lipid Oxidation during Pork Patties Shelf-Life.
- Author
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Vargas-Ramella M, Lorenzo JM, Zamuz S, Valdés ME, Moreno D, Balcázar MCG, Fernández-Arias JM, Reyes JF, and Franco D
- Abstract
A scarce amount of knowledge about the use of Colombian berry (CB) in meat products is available in the literature. This work studies the impact of the addition of CB extracts (CBE) on pork patties at three different concentrations in the range 250-750 mg/kg. CBE were characterized in terms of their polyphenolic profile and antioxidant activity [1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging capacity, half maximal inhibitory antioxidant concentration (IC
50 ), 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), ferric reducing antioxidant power assay (FRAP) and oxygen radical absorbance capacity (ORAC) tests)]. After pork patties elaboration, instrumental and sensorial colour, as well as lipid oxidation measured as thiobarbituric acid reactive substances assay (TBARS) values, were evaluated for 10 days of refrigerated storage in a modified atmosphere (80% O2 -20% CO2 ). The total anthocyanin composition represented 35% of the polyphenolic substances of the CBE, highlighting high contents in cyanidin derivatives. Additionally, other flavonoids (quercetin and kaempferol compounds) and phenolics acids, substances positively related to antioxidant activity, were identified and quantified. In addition, the incorporation of CBE resulted in improvements in colour and lipid stability of pork patties, especially for the highest concentration used. Our findings demonstrated that CBE could be added to pork patties without impairing their sensorial profile. Overall, our results indicate that the use of CBE as a source of natural antioxidant, natural colourant, or even as a functional ingredient could be promising, but more studies are necessary to confirm it.- Published
- 2021
- Full Text
- View/download PDF
37. Anxiety in neurosurgical patients undergoing nonurgent surgery during the COVID-19 pandemic.
- Author
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Doglietto F, Vezzoli M, Biroli A, Saraceno G, Zanin L, Pertichetti M, Calza S, Agosti E, Aliaga Arias JM, Assietti R, Bellocchi S, Bernucci C, Bistazzoni S, Bongetta D, Fanti A, Fioravanti A, Fiorindi A, Franzin A, Locatelli D, Pugliese R, Roca E, Sicuri GM, Stefini R, Venturini M, Vivaldi O, Zattra C, Zoia C, and Fontanella MM
- Subjects
- Adult, Aged, Female, Humans, Italy epidemiology, Male, Middle Aged, Prospective Studies, Anxiety epidemiology, Anxiety psychology, COVID-19 epidemiology, COVID-19 psychology, Neurosurgical Procedures psychology, Surveys and Questionnaires
- Abstract
Objective: The COVID-19 pandemic has forced many countries into lockdown and has led to the postponement of nonurgent neurosurgical procedures. Although stress has been investigated during this pandemic, there are no reports on anxiety in neurosurgical patients undergoing nonurgent surgical procedures., Methods: Neurosurgical patients admitted to hospitals in eastern Lombardy for nonurgent surgery after the lockdown prospectively completed a pre- and postoperative structured questionnaire. Recorded data included demographics, pathology, time on surgical waiting list, anxiety related to COVID-19, primary pathology and surgery, safety perception during hospital admission before and after surgery, and surgical outcomes. Anxiety was measured with the State-Trait Anxiety Inventory. Descriptive statistics were computed on the different variables and data were stratified according to pathology (oncological vs nononcological). Three different models were used to investigate which variables had the greatest impact on anxiety, oncological patients, and safety perception, respectively. Because the variables (Xs) were of a different nature (qualitative and quantitative), mostly asymmetrical, and related to outcome (Y) by nonlinear relationships, a machine learning approach composed of three steps (1, random forest growing; 2, relative variable importance measure; and 3, partial dependence plots) was chosen., Results: One hundred twenty-three patients from 10 different hospitals were included in the study. None of the patients developed COVID-19 after surgery. State and trait anxiety were reported by 30.3% and 18.9% of patients, respectively. Higher values of state anxiety were documented in oncological compared to nononcological patients (46.7% vs 25%; p = 0.055). Anxiety was strongly associated with worry about primary pathology, surgery, disease worsening, and with stress during waiting time, as expected. Worry about positivity to SARS-CoV-2, however, was the strongest factor associated with anxiety, even though none of the patients were infected. Neuro-oncological disease was associated with state anxiety and with worry about surgery and COVID-19. Increased bed distance and availability of hand sanitizer were associated with a feeling of safety., Conclusions: These data underline the importance of psychological support, especially for neuro-oncological patients, during a pandemic.
- Published
- 2020
- Full Text
- View/download PDF
38. On the importance of trip destination for modelling individual human mobility patterns.
- Author
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Lenormand M, Arias JM, San Miguel M, and Ramasco JJ
- Subjects
- Humans, Public Health, Travel
- Abstract
Obtaining insights into human mobility patterns and being able to reproduce them accurately is of the utmost importance in a wide range of applications from public health, to transport and urban planning. Still the relationship between the effort individuals will invest in a trip and the importance of its purpose is not taken into account in individual mobility models that can be found in the recent literature. Here, we address this issue by introducing a model hypothesizing a relation between the importance of a trip and the distance travelled. In most practical cases, quantifying such importance is undoable. We overcome this difficulty by focusing on shopping trips (for which we have empirical data) and by taking the price of items as a proxy. Our model is able to reproduce the long-tailed distribution in travel distances empirically observed and to explain the scaling relationship between distance travelled and item value found in the data.
- Published
- 2020
- Full Text
- View/download PDF
39. Interaction of cysteine and its derivatives with monolayers of dipalmitoylphosphatidylcholine.
- Author
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Arias JM, Díaz SB, Ben Altabef A, and Dupuy FG
- Subjects
- Adsorption, Cysteine analogs & derivatives, Molecular Structure, Particle Size, Surface Properties, 1,2-Dipalmitoylphosphatidylcholine chemistry, Cysteine chemistry
- Abstract
Molecular interactions between l-cysteine (Cys) and its ester derivatives (Cys
x ); l-cysteine ethyl ester (CE), l-cysteine methyl ester (CM) and N-acetyl l-cysteine (NAC) with 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) monolayers were investigated using Langmuir film balance technique. The effect of charge on monolayers made of cysteine and three ester derivatives with DPPC was investigated by working with un-buffered and buffered subphases. Also, the effects of cysteine derivatives interaction with DPPC monolayers were studied measuring the change in the surface tension upon aminoacid injection in the subphase whilst keeping lipid molecular density and lateral packing controlled. Cysteine and its ester derivatives showed interfacial activity reducing the air/water surface tension (πi ) by 4 mN m-1 . However, ester derivatives were able to insert into preformed DPPC monolayers at much higher surface pressures (Δπ), indicating a preferential interaction of Cysx with DPPC. The results indicate that, although the different derivatives of cysteine presented low surface activity, they were able to favourably interact with DPPC monolayers. Also, compression isotherms experiments in binary mixtures indicate that the more surface active compounds stabilized the gel phase of DPPC. The charge on cysteine and its derivatives did not increase the observed effects., (Copyright © 2019 Elsevier B.V. All rights reserved.)- Published
- 2019
- Full Text
- View/download PDF
40. Occurrence, thromboembolic risk, and mortality in Danish patients with cold agglutinin disease.
- Author
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Bylsma LC, Gulbech Ording A, Rosenthal A, Öztürk B, Fryzek JP, Arias JM, Röth A, and Berentsen S
- Subjects
- Adult, Aged, Aged, 80 and over, Anemia, Hemolytic, Autoimmune mortality, Denmark epidemiology, Disease Susceptibility, Female, Humans, Incidence, Male, Middle Aged, Mortality, Population Surveillance, Prevalence, Registries, Risk Assessment, Risk Factors, Thromboembolism mortality, Young Adult, Anemia, Hemolytic, Autoimmune complications, Anemia, Hemolytic, Autoimmune epidemiology, Thromboembolism epidemiology, Thromboembolism etiology
- Abstract
Cold agglutinin disease (CAD) is a rare form of autoimmune hemolytic anemia with limited epidemiological and clinical data. We used the Danish National Patient Registries to examine CAD occurrence and risk of thromboembolic events (TEs) and mortality in CAD patients compared with a matched cohort from the general population in Denmark. We identified 72 patients diagnosed with CAD and 720 matched controls between 1999 and 2013. For 2013, the most recent year of study, crude incidence of CAD was 0.18 per 100 000 inhabitants per year and prevalence was 1.26 per 100 000 inhabitants. Risk of TEs was higher in the CAD patient cohort than in the comparison cohort at 1 year (7.2% of CAD patients had TEs vs 1.9% of comparisons), 3 years (9.0% vs 5.3%), and 5 years (11.5% vs 7.8%) after the index date. The median survival was 8.5 years. CAD patients had increased mortality compared with the general population cohort (adjusted hazard ratio [aHR], 1.84; 95% confidence interval [CI], 1.10-3.06; P = .020), with the highest mortality observed during the first 5 years after diagnosis (aHR, 2.27; 95% CI, 1.32-3.89; P = .003). Mortality rates 1 and 5 years after diagnosis were 17% and 39% in the CAD group vs 3% and 18% in the comparison cohort, respectively. CAD is a rare illness characterized by increased risk of TEs and mortality., (© 2019 by The American Society of Hematology.)
- Published
- 2019
- Full Text
- View/download PDF
41. Homo digitalis and Contemporary Psychology.
- Author
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Cuartas-Arias JM
- Published
- 2019
- Full Text
- View/download PDF
42. Lactic acid production from sugarcane bagasse hydrolysates by Lactobacillus pentosus: Integrating xylose and glucose fermentation.
- Author
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Wischral D, Arias JM, Modesto LF, de França Passos D, and Pereira N Jr
- Subjects
- Fermentation physiology, Glucose metabolism, Xylose metabolism, Lactic Acid metabolism, Lactobacillus pentosus metabolism, Saccharum metabolism
- Abstract
Lactic acid, traditionally obtained through fermentation process, presents numerous applications in different industrial segments, including production of biodegradable polylactic acid (PLA). Development of low cost substrate fermentations could improve economic viability of lactic acid production, through the use of agricultural residues as lignocellulosic biomass. Studies regarding the use of sugarcane bagasse hydrolysates for lactic acid production by Lactobacillus spp. are reported. First, five strains of Lactobacillus spp. were investigated for one that had the ability to consume xylose efficiently. Subsequently, biomass fractionation was performed by dilute acid and alkaline pretreatments, and the hemicellulose hydrolysate (HH) fermentability by the selected strain was carried out in bioreactor. Maximum lactic acid concentration and productivity achieved in HH batch were 42.5 g/L and 1.02 g/L h, respectively. Hydrolyses of partially delignified cellulignin (PDCL) by two different enzymatic cocktails were compared. Finally, fermentation of HH and PDCL hydrolysate together was carried out in bioreactor in a hybrid process: saccharification and co-fermentation with an initial enzymatic hydrolysis. The high fermentability of these process herein developed was demonstrated by the total consumption of xylose and glucose by Lactobacillus pentosus, reaching at 65.0 g/L of lactic acid, 0.93 g/g of yield, and 1.01 g/L h of productivity. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 35: e2718, 2019., (© 2018 American Institute of Chemical Engineers.)
- Published
- 2019
- Full Text
- View/download PDF
43. The Trans Golgi Region is a Labile Intracellular Ca 2+ Store Sensitive to Emetine.
- Author
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Gallegos-Gómez ML, Greotti E, López-Méndez MC, Sánchez-Vázquez VH, Arias JM, and Guerrero-Hernández A
- Subjects
- Cell Line, Epithelial Cells metabolism, Humans, Myocytes, Cardiac metabolism, trans-Golgi Network metabolism, Antinematodal Agents pharmacology, Calcium metabolism, Emetine pharmacology, Epithelial Cells drug effects, Myocytes, Cardiac drug effects, trans-Golgi Network drug effects
- Abstract
The Golgi apparatus (GA) is a bona fide Ca
2+ store; however, there is a lack of GA-specific Ca2+ mobilizing agents. Here, we report that emetine specifically releases Ca2+ from GA in HeLa and HL-1 atrial myocytes. Additionally, it has become evident that the trans-Golgi is a labile Ca2+ store that requires a continuous source of Ca2+ from either the external milieu or from the ER, to enable it to produce a detectable transient increase in cytosolic Ca2+ . Our data indicates that the emetine-sensitive Ca2+ mobilizing mechanism is different from the two classical Ca2+ release mechanisms, i.e. IP3 and ryanodine receptors. This newly discovered ability of emetine to release Ca2+ from the GA may explain why chronic consumption of ipecac syrup has muscle side effects.- Published
- 2018
- Full Text
- View/download PDF
44. Reorganization of Hydration Water of DPPC Multilamellar Vesicles Induced by l-Cysteine Interaction.
- Author
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Arias JM, Tuttolomondo ME, Díaz SB, and Ben Altabef A
- Abstract
The aim of this study is to analyze the consequences of water redistribution on the structure and stability of phospholipid bilayers induced by cysteine (Cys). This interaction is studied with 1,2-dipalmitoyl- sn-glycero-3-phosphatidylcholine (DPPC) multilamellar vesicles in gel (30 °C) and liquid crystalline (50 °C) state; experimental studies were performed by means of Fourier transform infrared (FTIR) spectroscopy, Raman spectroscopy, and differential scanning calorimetry (DSC). The polar head sites of the lipid molecules to which water can bind are identified by competition with compounds that form hydrogen bonds, such as Cys. FTIR spectroscopy results revealed that there is a Cys interaction with the phospholipid head groups in the gel and liquid crystalline phases. Raman spectra were measured in the gel state. They were dominated by vibrations of the fatty acyl chains, with superposition of a few bands from the head group, and clearly showed that the S-H stretching band of Cys shifted to lower frequencies with a decrease in its force constant. DSC disclosed an overview of the behavior of the multilamellar vesicles in the working temperature range (30-50 °C) and showed how the increase of the molar ratios modified the environment of the polar head and the hydrocarbon chains. A loss of the pretransition ( T
P ) and an increase in the temperature of main transition ( Tm ) with increasing Cys/DPPC molar ratio were observed.- Published
- 2018
- Full Text
- View/download PDF
45. Functional histamine H 3 and adenosine A 2A receptor heteromers in recombinant cells and rat striatum.
- Author
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Márquez-Gómez R, Robins MT, Gutiérrez-Rodelo C, Arias JM, Olivares-Reyes JA, van Rijn RM, and Arias-Montaño JA
- Subjects
- Animals, CHO Cells, Cricetulus, HEK293 Cells, Humans, Neurons metabolism, Rats, Recombination, Genetic, Corpus Striatum metabolism, Receptor, Adenosine A2A metabolism, Receptors, Histamine H3 metabolism
- Abstract
In the striatum, histamine H
3 receptors (H3 Rs) are co-expressed with adenosine A2A receptors (A2A Rs) in the cortico-striatal glutamatergic afferents and the GABAergic medium-sized spiny neurons that originate the indirect pathway of the basal ganglia. This location allows H3 Rs and A2A Rs to regulate the striatal GABAergic and glutamatergic transmission. However, whether these receptors can physically interact has not yet been assessed. To test this hypothesis, a heteromer-selective in vitro assay was used to detect functional complementation between a chimeric A2A R302 -Gαqi4 and wild-type H3 Rs in transfected HEK-293T cells. H3 R activation with the agonist RAMH resulted in Ca2+ mobilization (pEC50 7.31 ± 0.23; maximal stimulation, Emax 449 ± 25% of basal) indicative of receptor heterodimerization. Functional H3 R-A2A R heteromers were confirmed by co-immunoprecipitation and observations of differential cAMP signaling when both receptors were co-expressed in the same cells. In membranes from rat striatal synaptosomes, H3 R activation decreased A2A R affinity for the agonist CGS-21680 (pKi values 8.10 ± 0.04 and 7.70 ± 0.04). Moreover, H3 Rs and A2A Rs co-immunoprecipitated in protein extracts from striatal synaptosomes. These results support the existence of a H3 R-A2A R heteromer with possible physiological implications for the modulation of the intra-striatal transmission., (Copyright © 2017 Elsevier Ltd. All rights reserved.)- Published
- 2018
- Full Text
- View/download PDF
46. Prevalence and Clinical Characteristics of the Restless Legs Syndrome (RLS) in Patients Diagnosed with Attention-Deficit Hyperactivity Disorder (ADHD) in Antioquia.
- Author
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Sierra Montoya AC, Mesa Restrepo SC, Cuartas Arias JM, and Cornejo Ochoa W
- Abstract
Introduction: Attention Deficit Hyperactivity Disorder (ADHD) is the most common behavioral issue for children. One of the sleeping disorders most frequently related to ADHD is the Restless Legs Syndrome (RLS), characterized by an irresistible urge to move the legs, something that is generally associated with paresthesias and motor restlessness. The prevalence rate of RLS in children diagnosed with ADHD is close to 18%, but in Colombia, these cases have been hardly studied., Objective: To determine the frequency of RLS, in children with ADHD., Methods: A cross-sectional study, filled out by parents of children diagnosed with ADHD, were analyzed. This questionnaire contained clinical criteria for classifying ADHD according to the DSM-IV, as well as diagnostic criteria for RLS by the National Institutes of Health (2003)., Results: A predominance rate of 65.6% in combined ADHD was observed in children with RLS criteria. Upon carrying out an exploratory data analysis, it was found that having a family history of RLS and belonging to the middle or low socioeconomic strata are conditions associated with the presence of RLS in children with ADHD, with a significant p (p < 0.000) and a PR of 4.47 (3.16-6.32)., Conclusions: The prevalence of RLS was similar to the findings of other clinical investigations. However, it highlights new prevalence values in relation to the comorbidity between ADHD and RLS, suggesting the need for new clinical and therapeutic alternatives amidst the presence of both syndromes.
- Published
- 2018
- Full Text
- View/download PDF
47. Differential homologous desensitization of the human histamine H 3 receptors of 445 and 365 amino acids expressed in CHO-K1 cells.
- Author
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García-Gálvez AM, Escamilla-Sánchez J, Flores-Maldonado C, Contreras RG, Arias JM, and Arias-Montaño JA
- Subjects
- Amino Acid Sequence, Animals, CHO Cells, Cell Membrane drug effects, Cell Membrane metabolism, Cricetinae, Cricetulus, Dose-Response Relationship, Drug, Gene Expression, Histamine Agonists metabolism, Histamine Agonists pharmacology, Humans, Protein Binding physiology, Protein Isoforms biosynthesis, Protein Isoforms genetics, Amino Acids biosynthesis, Amino Acids genetics, Receptors, Histamine H3 biosynthesis, Receptors, Histamine H3 genetics
- Abstract
Histamine H
3 receptors (H3 Rs) signal through Gαi/o proteins and are found in neuronal cells as auto- and hetero-receptors. Alternative splicing of the human H3 R (hH3 R) originates 20 isoforms, and the mRNAs of two receptors of 445 and 365 amino acids (hH3 R445 and hH3 R365 ) are widely expressed in the human brain. We previously showed that the hH3 R445 stably expressed in CHO-K1 cells experiences homologous desensitization. The hH3 R365 lacks 80 residues in the third intracellular loop, and in this work we therefore studied whether this isoform also experiences homologous desensitization and the possible differences with the hH3 R445 . In clones of CHO-K1 cells stably expressing similar receptor levels (211 ± 12 and 199 ± 16 fmol/mg protein for hH3 R445 and hH3 R365 , respectively), there were no differences in receptor affinity for selective H3 R ligands or for agonist-induced [35 S]-GTPγS binding to membranes and inhibition of forskolin-stimulated cAMP accumulation in intact cells. For both cell clones, pre-incubation with the H3 R agonist RAMH (1 μM) resulted in functional receptor desensitization, as indicated by cAMP accumulation assays, and loss of receptors from the cell surface and reduced affinity for the agonist immepip in cell membranes, evaluated by radioligand binding. However, functional desensitization differed in the maximal extent (96 ± 15% and 58 ± 8% for hH3 R445 and hH3 R365 , respectively) and the length of pre-exposure required to reach the maximum desensitization (60 and 30 min, respectively). Furthermore, the isoforms differed in their recovery from desensitization. These results indicate that the hH3 R365 experiences homologous desensitization, but that the process differs between the isoforms in time-course, magnitude and re-sensitization., (Copyright © 2017 Elsevier Ltd. All rights reserved.)- Published
- 2018
- Full Text
- View/download PDF
48. Genogram: tool for exploring and improving biomedical and psychological research.
- Author
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Cuartas Arias JM
- Published
- 2017
- Full Text
- View/download PDF
49. The naturally occurring mutation Y197C does not affect the expression or signaling of the human histamine H 3 receptor.
- Author
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Flores-Clemente C, Escamilla-Sánchez J, Arias JM, and Arias-Montaño JA
- Subjects
- Animals, CHO Cells, Cricetulus, Cyclic AMP biosynthesis, Drug Inverse Agonism, Histamine Agonists pharmacology, Histamine H3 Antagonists pharmacology, Humans, Mutation, Radioligand Assay, Receptors, Histamine H3 metabolism, Signal Transduction, Receptors, Histamine H3 genetics
- Abstract
There is evidence for genetic polymorphism within the human histamine H
3 receptor (hH3 R), and a Tyr to Cys exchange at position 197 (Y197C), located in the amino terminus of the fifth transmembrane domain, has been reported. In this work we compared the expression and the pharmacological and signaling properties of wild-type (hH3 RWT ) and mutant (hH3 RY197C ) receptors transiently expressed in CHO-K1 cells. The hH3 RY197C cDNA was created by overlap extension PCR amplification. Receptor expression and affinity were assessed by N-α-[methyl-3 H]-histamine binding to cell membranes and intact cells. Receptor function was evaluated by stimulation of [35 S]-GTPγS binding to cell membranes and by inhibition of forskolin-induced cAMP accumulation in intact cells. The hH3 RWT and hH3 RY197C were expressed at similar levels (761±68 and 663±66fmol/mg protein for membranes, and 13,434±1533 and 15,894±1884 receptors per cell, respectively). There were no significant differences in the affinities for H3 R agonists or antagonists/inverse agonists between the hH3 RWT and hH3 RY197C , and the H3 R agonist RAMH was similarly efficacious and potent to stimulate [35 S]-GTPγS binding and to inhibit forskolin-induced cAMP accumulation. These results indicate that the Y197C mutation does not affect the expression, ligand affinity or signaling of the human H3 receptor., (Copyright © 2017 Elsevier B.V. All rights reserved.)- Published
- 2017
- Full Text
- View/download PDF
50. Ionizing radiation from Chernobyl affects development of wild carrot plants.
- Author
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Boratyński Z, Arias JM, Garcia C, Mappes T, Mousseau TA, Møller AP, Pajares AJ, Piwczyński M, and Tukalenko E
- Subjects
- Chernobyl Nuclear Accident, Daucus carota growth & development, Daucus carota radiation effects, Germination radiation effects, Radiation, Ionizing, Seeds radiation effects
- Abstract
Radioactivity released from disasters like Chernobyl and Fukushima is a global hazard and a threat to exposed biota. To minimize the deleterious effects of stressors organisms adopt various strategies. Plants, for example, may delay germination or stay dormant during stressful periods. However, an intense stress may halt germination or heavily affect various developmental stages and select for life history changes. Here, we test for the consequence of exposure to ionizing radiation on plant development. We conducted a common garden experiment in an uncontaminated greenhouse using 660 seeds originating from 33 wild carrots (Daucus carota) collected near the Chernobyl nuclear power plant. These maternal plants had been exposed to radiation levels that varied by three orders of magnitude. We found strong negative effects of elevated radiation on the timing and rates of seed germination. In addition, later stages of development and the timing of emergence of consecutive leaves were delayed by exposure to radiation. We hypothesize that low quality of resources stored in seeds, damaged DNA, or both, delayed development and halted germination of seeds from plants exposed to elevated levels of ionizing radiation. We propose that high levels of spatial heterogeneity in background radiation may hamper adaptive life history responses.
- Published
- 2016
- Full Text
- View/download PDF
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