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14 results on '"Arden-Jacob, Jutta"'

3. Multiplex dye DNA sequencing in capillary gel electrophoresis by diode laser-based time-resolved fluorescence detection

Author

Liberwirth, Ulrike, Arden-Jacob, Jutta, Drexhage, Karl H., Herten, Dirk P., Muller, Ralph, Neumann, Michael, Schulz, Andreas, Siebert, Stefan, Sagner, Gregor, Klingel, Sven, Sauer, Markus, and Wolfrum, Jurgen

Subjects
DNA -- Analysis, Gel electrophoresis -- Innovations, Nucleotide sequence -- Methods, Chemistry
Abstract

A new one-lane, four-dye DNA sequencing method was developed which is based on time-resolved detection and identification of fluorescently labeled primers. For fluorescent labels, we used two newly synthesized rhodamine derivatives (MR200-1, JA169), a new oxazine derivative (JA242), and a commercially available cyanine dye (CY5). The dye fluorescence was excited by a pulsed diode laser emitting at 630 nm. The fluorescence decay was detected by an avalanche photodiode using a single-filter system. The dyes used here, so-called multiplex dyes, can be distinguished and identified via their fluorescence decay patterns. The DNA fragments were labeled at the primer using linkers of various lengths and positions. For separation of the enzymatically generated DNA fragments, capillary gel electrophoresis (CGE) with a 5% linear polyacrylamide gel was employed. On covalent attachment to oligonucleotides, the dyes exhibit fluorescence decay times of 3.7 (MR200-1), 2.9 (JA169), 2.4 (JA242), and 1.6 ns (CY5) measured during CGE. The CGE mobility of the labeled DNA fragments could be controlled and nearly equalized by the coupling position and the linker length. First, time-resolved, one-lane, four-dye DNA sequencing runs in CGE are presented. The sequence information of 660 bp was determined with a probability of correct classification of >90%. This result was obtained directly from the raw data without any of the mobility corrections that are necessary with other methods.

Published
1998

4. Cancer Diagnostics by Multiparameter Fluorescence Image Spectroscopy : A Bioinformatic Classifier Trained on Cultured Immunostained Cells

Author

Zentis, Peter D., Frohnapfel, Manuel, Rantanen, Ville, Weidtkamp-Peters, Stefanie, Felekyan, Suren, Kuehnemuth, Ralf, Sisamakis, Evangelos, Xu, Lei, Perols, Anna, Arden-Jacob, Jutta, Zilles, Alexander, Oberlaender, Martina, Fritzsche, Britta, Nygren, Per-Åke, Eriksson Karlström, Amelie, Hautaniemi, Sampsa, Drexhage, Karl-Heinz, Auer, Gert, Habermann, Jens K., Widengren, Jerker, Seidel, Claus A. M., Zentis, Peter D., Frohnapfel, Manuel, Rantanen, Ville, Weidtkamp-Peters, Stefanie, Felekyan, Suren, Kuehnemuth, Ralf, Sisamakis, Evangelos, Xu, Lei, Perols, Anna, Arden-Jacob, Jutta, Zilles, Alexander, Oberlaender, Martina, Fritzsche, Britta, Nygren, Per-Åke, Eriksson Karlström, Amelie, Hautaniemi, Sampsa, Drexhage, Karl-Heinz, Auer, Gert, Habermann, Jens K., Widengren, Jerker, and Seidel, Claus A. M.

Abstract

QC 20130506

Published
2013
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5. Cancer Diagnostics by Multiparameter Fluorescence Image Spectroscopy: A Bioinformatic Classifier Trained on Cultured Immunostained Cells

Author

Zentis, Peter D., primary, Frohnapfel, Manuel, additional, Rantanen, Ville, additional, Weidtkamp-Peters, Stefanie, additional, Felekyan, Suren, additional, Kühnemuth, Ralf, additional, Sisamakis, Evangelos, additional, Xu, Lei, additional, Perols, Anna, additional, Arden-Jacob, Jutta, additional, Zilles, Alexander, additional, Oberländer, Martina, additional, Fritzsche, Britta, additional, Nygren, Per-Åke, additional, Eriksson Karlström, Amelie, additional, Hautaniemi, Sampsa, additional, Drexhage, Karl-Heinz, additional, Auer, Gert, additional, Habermann, Jens K., additional, Widengren, Jerker, additional, and Seidel, Claus A.M., additional

Published
2013
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9. Multiplex Dye DNA Sequencing in Capillary Gel Electrophoresis by Diode Laser-Based Time-Resolved Fluorescence Detection

Author

Lieberwirth, Ulrike, primary, Arden-Jacob, Jutta, additional, Drexhage, Karl H., additional, Herten, Dirk P., additional, Müller, Ralph, additional, Neumann, Michael, additional, Schulz, Andreas, additional, Siebert, Stefan, additional, Sagner, Gregor, additional, Klingel, Sven, additional, Sauer, Markus, additional, and Wolfrum, Jürgen, additional

Published
1998
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11. Design of multiplex dyes for the detection of different biomolecules

Author

Sauer, Markus, primary, Han, Kyung-Tae, additional, Ebert, Volker, additional, Mueller, Ralph, additional, Schulz, Andreas, additional, Seeger, Stefan, additional, Wolfrum, Juergen M., additional, Arden-Jacob, Jutta, additional, Deltau, Gerhard, additional, Marx, Nicolaas J., additional, and Drexhage, Karl-Heinz, additional

Published
1994
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12. Biodiagnostics with multiplex dyes

Author

Seeger, Stefan, primary, Arden-Jacob, Jutta, additional, Deltau, Gerhard, additional, Drexhage, Karl-Heinz, additional, Han, Kyung-Tae, additional, Koellner, Malte, additional, Mueller, Ralph, additional, Sauer, Markus, additional, and Wolfrum, Juergen M., additional

Published
1994
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13. Design of Multiplex Dyes

Author

Sauer, Markus, primary, Schulz, Andreas, additional, Seeger, Stefan, additional, Wolfrum, Jürgen, additional, Arden‐Jacob, Jutta, additional, Deltau, Gerhard, additional, and Drexhage, Karl H., additional

Published
1993
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