1. Transformation of Cardamom with the RNA Dependent RNA Polymerase Gene of Cardamom mosaic virus
- Author
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R. Usha, C. Manohari, S. Backiyarani, T Jebasingh, and Archana Somanath
- Subjects
Expression vector ,food.ingredient ,biology ,business.industry ,fungi ,Elettaria cardamomum ,food and beverages ,RNA-dependent RNA polymerase ,RNA ,Bialaphos ,Molecular biology ,Biotechnology ,Transformation (genetics) ,chemistry.chemical_compound ,food ,chemistry ,biology.protein ,business ,Gene ,Polymerase - Abstract
Cardamom (Elettaria cardamomum Maton) is an important spice crop. It is affected by Cardamom mosaic virus (CdMV). In order to make cardamom plants resistant to CdMV by the pathogen-derived resistance approach, the RNA dependent RNA polymerase gene (NIb) of CdMV in the plant expression vector pAHC17, was introduced into cardamom embryogenic calli along with GFP-BAR by particle bombardment. Transformants were selected on a medium containing bialaphos and the presence of NIb and gfp genes in cardamom plants were confirmed by PCR, Southern hybridization and GFP expression.
- Published
- 2017
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