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6 results on '"Arbatsky, N.P."'

4. Mucin-type glycoprotein from Drosophila melanogasterembryonic cells: characterization of carbohydrate component

Author

Kramerov, A.A., Arbatsky, N.P., Rozovsky, Y.M., Mikhaleva, E.A., Polesskaya, O.O., Gvozdev, V.A., and Shibaev, V.N.

Abstract

A secreted glycoprotein (GP) with apparent molecular mass of 90 kDa produced by cultured embryonic cells of Drosophila melanogasterwas isolated and partially characterized. GP is enriched by Ser + Thr and Pro residues that constitute up to 30% of the total number of amino acids. An abundant carbohydrate moiety (40% of molecular mass) is mainly represented by vertebrate mucin-type O-linked disaccharide units Gal(β1–3)-GalNAc, occupying about a half of the total number of Ser + Thr residues and rendering the GP molecule high resistance to protease action. A few of N-glycans are also present in GP. These characteristics allow to consider the DrosophilaGP (termed ‘mucin-D’) as a first representative of invertebrate mucin-type glycoproteins.

Published
1996
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5. Characterization of the primary structure and the microheterogeneity of the carbohydrate chains of porcine blood-group H substance by 500-MHz 1H-NMR spectroscopy

Author

Vliegenthart, J.F.G., Halbeek, H. van, Dorland, L., Kochetkov, N.K., Arbatsky, N.P., and Derevitskaya, V.A.

Subjects
Scheikunde
Abstract

In blood-group H substance from pig stomach cell linings, carbohydrate structures are known to occur which are O-glycosidically linked via GalNAc to Ser or Thr of the polypeptide backbone. They have in common the Gal(beta1->3)GalNAc core unit, which bears one or more N-acetyllactosamine branches. The latter are terminated either by Fuc in (alpha1->2) linkage or by GlcNAc in (alpha1->44) linkage to Gal. Both terminal sequences are considered to be porcine blood-group H antigenic determinants. Employing 500-MHz 1H-NMR spectroscopy, the spectral features of the oligosaccharide-alditols corresponding to these chains were established. Insight could be gained into the microheterogeneity displayed by some of the alditol fractions as to the distribution of the terminating Fuc and GlcNAc residues over the various branches. Such information can hardly be obtained using other approaches.

Published
1982

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