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The propagation of Givotia rottlerformis Griff is troublesome as consequence of it is long seed dormancy associated with poor seed germination. The breaking of seed dormancy is administered by environmental factors, including temperature, light, nitrate, and smoke constituents. Givotia seeds were treated with Scarification, Chilling, Hot water and Growth regulators in order to break its dormancy and enhance the germination percentage. The maximum seed germination percentage (50%) was obtained when seeds treated by Mechanical Scarification.

L-Asparaginase appears to be highly effective especially in children with newly diagnosed Acute lymphoblastic leukemia. Prolonged L-Asparaginase intensification improved the outcome significantly. Nowadays L-Asparaginase is an essential drug that is used to treat children with Acute lymphoblastic leukemia all over the world. Its potential is now well established, as it has remarkably induced remission in most of the patients suffering with this disease. This therapy has brought a major breakthrough in modern oncology and with the development of its new functions, a great demand for L-Asparaginase is expected in the coming years. Keeping the potential of this particular enzyme in cancer treatment, the present work is planned with an intention of optimizing the production of L-Asparaginase both by conventional as well as by genetic engineering techniques. Pectobacterium carotovorum is selected for the present work based on its potentiality in cancer treatment and due to less glutaminase activity compared to the enzyme sourced from E. coli. Microbial culture was procured from MTCC, Chandigarh and screened for L-Asparaginase production in plate method and identified the activity based on the formation of pink color zone after overnight incubation.

The Aspergillus niger was tested for its ability to produce invertase on natural agricultural residues. Orange peel was selected as substrate due to its high peak enzyme activity, the maximum invertase activity was obtained in preliminary studies by incubating flasks containing orange peel as substrate supplemented with sucrose (1.0% w/v), yeast extract (1% w/v), and 4 ml of A. niger conidial suspension for 96 hours at 30°C.