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Several fruit fly species are pest of fruit production in subtropical and tropical countries such as Brazil. Data about the fruit fly communities are relevant for understanding ecological aspects such as population dynamics, abundance, richness, and diversity. In these surveys, Multilure traps were hung about 2 m height accessible to collectors. Practically there are no data on the dynamics of fruit fly species in higher positions. Thus, a comparative analysis was performed to assess the influence of the Multilure traps height, 2 m and 10 m, to collect Anastrephaspecies in the agricultural and forest environments. The abundance of Anastrephaspecies was compared using GLM multivariate analysis and species diversity using Hill numbers for each tested height. The heat map graph, based on the Euclidean distance, was used to assess the interaction of species at each height. A total of 1080 females of Anastrephawere captured, with 722 and 358 specimens collected in traps hung at 10 and 2 m height, respectively. Fourteen species were collected, with most identified as A. fraterculus(Wiedemann) (lato sensu) (n = 435) and A. obliqua(Macquart) (n = 388). Species diversity of Anastrephawas greater at 2 m than at 10 m, as were the values of diversity indices corresponding to richness (q = 0), Shannon (q = 1), and Simpson (q = 2). We conclude that evaluations of aspects related to the distribution of Anastrephaspecies at different heights should be adopted in fruit fly population monitoring programs as a management strategy for pest species.