1. Determination of the Cryo-EM Structure of ATG9 from Arabidopsis thaliana.
- Author
-
Zhai L and Zhang W
- Subjects
- Autophagosomes metabolism, Autophagosomes ultrastructure, Autophagy, Membrane Proteins metabolism, Membrane Proteins chemistry, Membrane Proteins ultrastructure, Cryoelectron Microscopy methods, Arabidopsis metabolism, Arabidopsis ultrastructure, Arabidopsis Proteins metabolism, Arabidopsis Proteins chemistry, Arabidopsis Proteins ultrastructure, Autophagy-Related Proteins metabolism, Autophagy-Related Proteins chemistry
- Abstract
Macroautophagy/autophagy is a highly conserved process for the degradation of cellular components and plays an essential role in cellular homeostasis maintenance. During autophagy, specialized double-membrane vesicles known as autophagosomes are formed and sequester cytoplasmic cargoes and deliver them to lysosomes or vacuoles for breakdown. Central to this process are autophagy-related (ATG) proteins, with the ATG9-the only integral membrane protein in this core machinery-playing a central role in mediating autophagosome formation. Recent years have witnessed the maturation of cryo-electron microscopy (cryo-EM) and single-particle analysis into powerful tools for high-resolution structural determination of protein complexes. These advancements have significantly deepened our understanding of the intricate molecular mechanisms underlying autophagosome biogenesis. In this study, we present a protocol detailing the acquisition of the three-dimensional structure of ATG9 from Arabidopsis thaliana. The structural resolution achieved 7.8 Å determined by single-particle cryo-electron microscopy (cryo-EM)., (© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
- Published
- 2024
- Full Text
- View/download PDF