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John C Araujo,1 Geralyn C Trudel,2 Prashni Paliwal3 1Department of Genitourinary Medical Oncology, Division of Cancer Medicine, The University of Texas MD Anderson Cancer Center, Houston, TX, USA; 2Bristol-Myers Squibb, Montreal, Quebec, Canada; 3Bristol-Myers Squibb, Wallingford, CT, USA Abstract: Dasatinib is a potent oral tyrosine kinase inhibitor which targets several kinases, including the SRC family kinases. SRC family kinases have been implicated in androgen therapy resistance that often develops in metastatic castration-resistant prostate cancer (mCRPC), which drives the need for non-androgen targeting therapies. This article describes the preclinical rationale for the use of combination dasatinib and docetaxel therapy in mCRPC, and highlights the results of a phase I–II trial in which 46 patients with mCRPC, treated with a regimen of dasatinib and docetaxel, demonstrated improvements in bone scans, high rates of soft tissue responses, and modulation of markers of bone turnover. This brief report discusses in detail follow-up data on two patients who remain alive after >2.5 years on dasatinib single-agent therapy after discontinuing docetaxel treatment. Keywords: case study, dasatinib, docetaxel, prostate cancer, targeted therapy

This work aims to develop a bio-based fibrous material that is able to adsorb and degrade chemical and biological hazardous agents. Thus, cellulosic fabrics (flax) were functionalized with chitosan (CS) and poly(ethylene oxide) (PEO) electrospun nanofibers doped with titanium dioxide (TiO 2 ) and cerium dioxide (CeO 2 ) nanoparticles (NPs). The electrospray deposition of these NPs was also tested. The TiO 2 NPs were synthesized using a very straightforward precipitation method. The successful synthesis was confirmed by Scanning Transmission Electron Microscopy (STEM) and Attenuated Total Reflectance-Fourier-Transform Infrared Spectroscopy (ATR-FTIR). The functionalization of the fabrics with the NPs and the nanofibers doped with NPs was proved by Field Emission Scanning Electron Microscopy (FESEM), ATR-FTIR, and Ground-State Diffuse Reflectance (GSDR). The developed samples presented antibacterial activity against Staphylococcus aureus (ATCC 6538) and Escherichia coli (ATCC 434), reaching values of 99.97 and 100.0 %, respectively. The degradation of methylene blue (MB) and dimethyl methylphosphonate (DMMP) was evaluated. The best samples were able to decompose 97.82 % of DMMP. The wash durability of the nanocoating was also tested. The developed fibrous structures show great potential for personal protection applications., Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Diana P. Ferreira reports financial support was provided by Foundation for Science and Technology. Joana C. Araujo reports financial support was provided by Foundation for Science and Technology. Pilar Teixeira reports financial support was provided by Foundation for Science and Technology. Catia L. Graca reports financial support was provided by Foundation for Science and Technology. Olivia Salome G. P. Soares reports was provided by Foundation for Science and Technology. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)

Anhydrous ammonia has seen a rapid increase in demand due to recent developments in clean energy technologies. As it is a potential carrier of hydrogen, the transportation industry is currently facing significant logistic challenges as well as safety risks. Based on the causes and effects, this study categorizes incident patterns based on the historical incidents from 1971 to 2021 in the United States during transportation. Analysis of temporal patterns revealed that government regulations and improvement of safety infrastructures have made the biggest impact on lowering incident rates. Spatial analysis methods are also applied to understand the relationships between these incidents and spatial factors, such as land area, number of ammonia production facilities, total average production capacity, and total length of freight railway and highway in each US state. The spatial and temporal patterns and interpretations provide safety references to manage the growing hazardous transport in clean energy., (© 2024 The Author(s). Risk Analysis published by Wiley Periodicals LLC on behalf of Society for Risk Analysis.)

Pineapple leaves can provide competitive and high-quality fibers for textile purposes. Despite pineapple being cultivated in the Portugues islands there is still a technology gap for the extraction and treatment of Pineapple Leaf Fibers (PALF) in Europe. Since Azorean Pineapple differs significantly from other plants in the bromeliad family, the properties and characterization of its leaf fibers were explored for the first time. Long fibers have been extracted by hand scraping and compared to biological retting at 25 °C for different time periods. It was explored the properties of PALF from plants of different ages (11- and 18-months) and from different zones of the leaves (beginning, middle, and tip). Physical-mechanical properties of Azores PALF were determined, including diameter, linear density, strength, Young's modulus, and elongation at break and characterized by ATR-FTIR, XRD, TGA/DTG, and FESEM to understand their chemical and morphological characteristics. While slight differences were observed between different ages, variations in physical-mechanical properties were notable among fibers extracted from different leaf positions. Extraction of Azores PALF through 25 °C biological retting for 14 days effectively eliminated non-fibrous matter and produced the thinnest and strongest fibers. These fibers ranged between 34.9 and 168.3 μm in diameter, 1.39 and 7.07 tex in linear mass density, 37-993 MPa in tensile strength, 1.0-3.9 % in elongation at break, and 2.4-21.8 GPa in Young's modulus., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2024 The Authors. Published by Elsevier Ltd.)

Genetic group, age at entry into confinement and at slaughter, are characteristics that have an important influence on lamb performance and carcass. The aim of this study was to evaluate the performance, carcass characteristics and non-carcass components from different genetic groups (Santa Inês and ½ Dorper x ½ Santa Inês) sheep, submitted to different feedlot entry and exit strategies. Were used 72 lambs males and castrated; 36 Santa Inês (SI) and 36 crossbred (Dorper x Santa Inês-DSI), with 6 months of average initial age. The groups were established in a completely randomized experimental design, in a 2x3x4 factorial arrangement, from the combination of genetic groups (GG), body weight at the beginning of confinement (WBC) and length of stay in confinement (LSC). The body weight classes at the beginning of confinement were: light (25 kg), intermediate (28 kg) and heavy (31 kg), for Santa Inês and crossbreeds, respectively. Slaughters were carried out every 28 days of confinement, in four LSC: 0, 28, 56 and 84 days. The GG did not influence performance, carcass and non-carcass component traits of lambs (p > 0.05). There was an effect of the WBC on the weights: final (FW), metabolic (MW), body at slaughter (BWS), empty body (EBW), hot carcass (HCY) and cold (CCW), loin, shoulder, leg musculature; loin eye area (LEA) and loin fat (p < 0.05). There was also an effect on LSC, for FW, average daily weight gain (ADG), MW, weight and yield of body components, weight of cuts and tissue ratio components of cuts (p < 0.05). In non-carcass components, effect on full and empty weight of: omasum, rumen-reticulum, small intestine; empty large intestine, liver and kidneys, paws and skin, and perirenal, pelvic and inguinal fat (p < 0.05). Interaction double effect on the tissue muscle/fat:bone ratio (MF:B) and for the full omasal component (p < 0.05). And triple interaction effect for ADG, full omasum and perirenal fat (p < 0.05). Weight at the beginning of confinement and confinement time are the characteristics that most influence performance, quantitative characteristics of carcass and non-carcass components. Regardless of the genetic group and age class, the animals reach the same weight after 84 days of confinement. Thus, the confinement of heavier lambs (31 kg) can be a profitable alternative, as they presented the highest weights for the most commercially valued cuts (shank and loin). The confinement strategy must adapt to market situations., Competing Interests: The authors have declared that no competing interests exist., (Copyright: © 2023 Pereira et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)

Cancer remains one of the most challenging health problems worldwide, and localized therapeutic approaches based on micro/nanofibers have shown potential for its treatment. Micro/nanofibers offer several advantages as a drug delivery system, such as high surface area, tunable pore size, and sustained release properties, which can improve drug efficacy and reduce side effects. In addition, functionalization of these fibers with nanoparticles can enhance their targeting and therapeutic capabilities. Localized delivery of drugs and/or other therapeutic agents via micro/nanofibers can also help to overcome the limitations of systemic administration, such as poor bioavailability and off-target effects. Several studies have shown promising results in preclinical models of cancer, including inhibition of tumor growth and improved survival rates. However, more research is needed to overcome technical and regulatory challenges to bring these approaches to clinical use. Localized therapeutic approaches based on micro/nanofibers hold great promise for the future of cancer treatment, providing a targeted, effective, and minimally invasive alternative to traditional treatments. The main focus of this review is to explore the current treatments utilizing micro/nanofibers, as well as localized drug delivery systems that rely on fibrous structures to deliver and release drugs for the treatment of cancer in a specific area.

The COVID-19 pandemic has caused a global health crisis, and wastewater-based epidemiology (WBE) has emerged as an important tool to assist public health decision-making. Recent studies have shown that the SARS-CoV-2 RNA concentration in wastewater samples is a reliable indicator of the severity of the pandemic for large populations. However, few studies have established a strong correlation between the number of infected people and the viral concentration in wastewater due to variations in viral shedding over time, viral decay, infiltration, and inflow. Herein we present the relationship between the number of COVID-19-positive patients and the viral concentration in wastewater samples from three different hospitals (A, B, and C) in the city of Belo Horizonte, Minas Gerais, Brazil. A positive and strong correlation between wastewater SARS-CoV-2 concentration and the number of confirmed cases was observed for Hospital B for both regions of the N gene (R = 0.89 and 0.77 for N1 and N2, respectively), while samples from Hospitals A and C showed low and moderate correlations, respectively. Even though the effects of viral decay and infiltration were minimized in our study, the variability of viral shedding throughout the infection period and feces dilution due to water usage for different activities in the hospitals could have affected the viral concentrations. These effects were prominent in Hospital A, which had the smallest sewershed population size, and where no correlation between the number of defecations from COVID-19 patients and viral concentration in wastewater was observed. Although we could not determine trends in the number of infected patients through SARS-CoV-2 concentrations in hospitals' wastewater samples, our results suggest that wastewater monitoring can be efficient for the detection of infected individuals at a local level, complementing clinical data., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier B.V. All rights reserved.)

Wastewater treatment plants (WWTPs) are recognized as important sources of Antibiotic Resistant Bacteria (ARBs) and Antibiotic Resistant Genes (ARGs), and might play a role in the removal and dissemination of antimicrobial resistance (AMR) in the environment. Detailed information about AMR removal by the different treatment technologies commonly applied in urban WWTPs is needed. This study investigated the occurrence, removal and characterization of ARBs in WWTPs employing different technologies: WWTP-A (conventional activated sludge-CAS), WWTP-B (UASB reactor followed by biological trickling filter) and WWTP-C (modified activated sludge followed by UV disinfection-MAS/UV). Samples of raw sewage (RI) and treated effluent (TE) were collected and, through the cultivation-based method using 11 antibiotics, the antibiotic resistance profiles were characterized in a one-year period. MAS was effective in reducing ARB counts (2 to 3 log units), compared to CAS (1 log unit) and UASB/BTF (0.5 log unit). The composition of cultivable ARB differed between RI and TE samples. Escherichia was predominant in RI (56/118); whilst in TE Escherichia (31/118) was followed by Bacillus (22/118), Shigella (14/118) and Enterococcus (14/118). Most of the isolates identified (370/394) harboured at least two ARGs and in over 80 % of the isolates, 4 or more ARG (int1, blaTEM, TetA, sul1 and qnrB) were detected. A reduction in the resistance prevalence was observed in effluents after CAS and MAS processes; whilst a slight increase was observed in treated effluents from UASB/BTF and after UV disinfection stage. The multi-drug resistance (MDR) phenotype was attributed to 84.3 % of the isolates from RI (27/32) and 63.6 % from TE (21/33) samples and 52.3 % of the isolates (34/65) were resistant to carbapenems (imipenem, meropenem, ertapenem). The results indicate that treated effluents are still a source for MDR bacteria and ARGs dissemination to aquatic environments. The importance of biological sewage treatment was reinforced by the significant reductions in ARB counts observed. However, implementation of additional treatments is needed to mitigate MDR bacteria release into the environment., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier B.V. All rights reserved.)

This study assessed the bacterial community composition of a drinking water system (DWS) serving a mid-sized city (120,000 inhabitants) in Brazil. Water samples, including raw and treated water, were collected at seven points throughout the DWS. DNA was extracted and analysed using high-throughput sequencing (Ion Torrent). Free chlorine and turbidity were measured in situ. Results showed that the highest relative abundance of 16S rRNA genes was from phyla Proteobacteria, followed by Bacteroidetes and Actinobacteria. The next most abundant phylum was Cyanobacteria, represented by Arthronema, Calothrix, and Synechococcus. An interesting observation was that the DNA-based analysis suggested a bacterial community change in the distribution network, with treated reservoir water being very different from the network samples. This suggests active microbiology within the distribution network and a tendency for bacterial diversity to decrease after chlorine disinfection but increase after pipeline distribution. In raw water, a predominance of Proteobacteria was observed with reduced Cyanobacteria, showing a negative correlation. In treated water, Proteobacteria were negatively correlated with Bacteroidetes. Finally, 16S rRNA genes from Firmicutes (especially Staphylococcus) had a high abundance in the chlorinated water, which may indicate the phylum's resistance to chlorine residuals. Opportunistic pathogens, e.g., Mycobacteria, Legionella, and Staphylococcus, were also observed.

Tendon and ligament traumatic injuries are among the most common diagnosed musculoskeletal problems. Such injuries limit joint mobility, reduce musculoskeletal performance, and most importantly, lower people's comfort. Currently, there are various treatments that are used to treat this type of injury, from surgical to conservative treatments. However, they're not entirely effective, as reinjures are frequent and, in some cases, fail to re-establish the lost functionality. Tissue engineering (TE) approaches aim to overcome these disadvantages by stimulating the regeneration and formation of artificial structures that resemble the original tissue. Fabrication and design of artificial fibrous scaffolds with tailored mechanical properties are crucial for restoring the mechanical function of the tissues. Recently, polymeric nanofibers produced by wetspinning have been largely investigated to mimic, repair, and replace the damaged tissue. Wetspun fibrous structures are extensively used due to their exceptional properties, such as the ability to mimic the native tissue, their biodegradability and biocompatibility, and good mechanical properties. In this review, the tendon and ligament structure and biomechanics are presented. Then, promising wetspun multifunctional fibrous structures based on biopolymers, more specifically polyhydroxyalkanoates (PHA), polycaprolactone (PCL), and polyethylenes, will be discussed, as well as reinforcing agents such as cellulose nanocrystals (CNC), nanoparticles, and growth factors.

Wastewater treatment plants (WWTPs) have been widely investigated in Europe, Asia and North America regarding the occurrence and fate of antibiotic resistance (AR) elements, such as antibiotic resistance genes (ARGs), mobile genetic elements (MGEs) and antibiotic resistant bacteria and pathogens. However, monitoring data about AR elements in municipal WWTPs in Brazil are scarce. This study investigated the abundance of intI1, five ARGs (sul1, tetA, blaTEM, ermB and qnrB) and 16S rRNA in raw and treated wastewater of three WWTPs, using different sewage treatments named CAS (Conventional activated sludge), UASB/BTF (UASB followed by biological trickling filter) and MAS/UV (modified activated sludge with UV disinfection stage). Bacterial diversity and the presence of potentially pathogenic groups were also evaluated, and associations between genetic markers and the bacterial populations were presented. All WWTPs decreased the loads of genetic markers finally discharged to receiving water bodies and showed no evidence of being hotspots for antimicrobial resistance amplification in wastewater, since the abundances of intI1 and ARGs within the bacterial population were not increased in the treated effluents. UASB/BTF showed a similar performance to that of the CAS and MAS/UV, reinforcing the sanitary and environmental advantages of this biological treatment, widely applied for wastewater treatment in warm climate regions. Bacterial diversity and richness increased after treatments, and bacterial communities in wastewater samples differed due to catchment areas and treatment typologies. Potential pathogenic population underwent considerable decrease after the treatments; however, strong significant correlations with intI1 and ARGs revealed potential multidrug-resistant pathogenic bacteria (Aeromonas, Arcobacter, Enterobacter, Escherichia-Shigella, Stenotrophomonas and Streptococcus) in the treated effluents, although in reduced relative abundances. These are contributive results for understanding the fate of ARGs, MGEs and potential pathogenic bacteria after wastewater treatments, which might support actions to mitigate their release into Brazilian aquatic environments in the near future., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier B.V. All rights reserved.)

Coronavirus pandemic started in March 2020 and since then has caused millions of deaths worldwide. Wastewater-based epidemiology (WBE) can be used as an epidemiological surveillance tool to track SARS-CoV-2 dissemination and provide warning of COVID-19 outbreaks. Considering that there are public places that could be potential hotspots of infected people that may reflect the local epidemiological situation, the presence of SARS-CoV-2 RNA was analyzed by RT-qPCR for approximately 16 months in sewage samples from five public places located in the metropolitan area of Belo Horizonte, MG, Brazil: the sewage treatment plant of Confins International Airport (AIR), the main interstate bus terminal (BUS), an upscale shopping centre (SHC1), a popular shopping centre (SHC2) and a university institute (UNI). The results were compared to those of the influent sewage of the two main sewage treatment plants of Belo Horizonte (STP1 and STP2). Viral monitoring in the STPs proved to be an useful regional surveillance tool, reflecting the trends of COVID-19 cases. However, the viral concentrations in the samples from the selected public places were generally much lower than those of the municipal STPs, which may be due to the behaviour of the non-infected or asymptomatic people, who are likely to visit these places relatively more than the symptomatic infected ones. Among these places, the AIR samples presented the highest viral concentrations and concentration peaks were observed previously to local outbreaks. Therefore, airport sewage monitoring can provide an indication of the regional epidemiological situation. For the other places, particularly the UNI, the results suggested a greater potential to detect the infection and trace cases especially among employees and regular attendees. Taken together, the results indicate that for a regular and permanent sentinel sewage surveillance the sewage from STPs, AIR and UNI could be monitored., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier B.V. All rights reserved.)

The partial nitritation/anammox process (PN/A) could be a promising alternative for nitrogen removal from high-strength wastewater. There is, however, a lack of information about suitable aeration and temperature for PN/A in single-stage reactors for high-strength wastewater, such as food waste (FW) digestate treatment. To this end, a laboratory-scale (10 L) partial nitritation/anammox sequencing batch reactor was operated for more than 230 days under four different intermittent aeration strategies and temperature variations (35°C and ambient temperature - 26-29°C) to investigate the feasibility of nitrogen removal from real FW digestate. High ammonium ( NH 4 + -N) and total nitrogen (TN) removal median efficiencies of 81 and 63%, respectively (corresponding to median NH 4 + -N and TN loads removed of 76 and 67 g.m -3 .d -1 ), were achieved when the aeration strategy comprised by 7 min/14 min off and an airflow rate of 0.050 L.min -1 .L reactor -1 was applied. Nitrogen removal efficiencies were not affected by temperature variations in southeastern Brazil. COD, chloride and organic nitrogen (520, 239 and 102.8 mg.L -1 , respectively) did not prevent PN/A. Changes of the bacterial community in response to aeration strategies were observed. Candidatus Brocadia dominated most of the time being more resistant to aeration and temperature changes than Candidatus Jettenia . This study demonstrated that optimizations of anoxic periods and airflow rate support PN/A with high nitrogen removal from FW digestate.

Gullies are a major threat to ecosystems, potentially leading to land degradation, groundwater depletion, crop loss, debris flow, and desertification. Gullies are also characterized by having a fast development and turning into primary sediment sources. Despite their impact, we have but scarce understanding of how gully erosion evolves and how to model it. In this paper, we propose a new gully erosion model that is based on the classical premise of net shear stress, i.e., hydraulic shear stress minus critical (resistant) shear stress, to calculate detachment rates. In order to calculate hydraulic shear stress, we developed a new equation derived from the principle of minimum cross-entropy; it was validated with laboratory measures from the literature with a Nash-Sutcliffe Efficiency of 0.95. Soil samples were analysed in the laboratory to assess critical shear stress and other soil properties. The novel gully erosion model was implemented in three gully impacted locations with catchment areas ranging from 10 -2 to 10 +1 ha. To assess channel geometry and eroded volumes, we used Unmanned Aerial Vehicle and Structure-from-Motion technique. The model successfully estimated long-term erosion rates, its efficiency was 0.77, and it is recommended for catchments up to 8 ha. Therefore, the new model provides planners and stakeholders with a tool to assess gully erosion, sediment yield and geometry in most areas., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier B.V. All rights reserved.)

Wastewater tertiary treatment has been pointed out as an effective alternative for reducing the concentration of antibiotic resistant bacteria and genes (ARB and ARGs) in wastewaters. The present work aimed to build on the current knowledge about the effects of activated sludge and UV irradiation on antibiotic resistance determinants in biologically treated wastewaters. For that, the microbial community and ARGs' composition of samples collected after preliminary (APT), secondary (AST), and tertiary (ATT) treatments in a full-scale wastewater treatment plant using a modified activated sludge (MAS) system followed by an UV stage (16 mJ/cm 2 ) were investigated through culture-dependent and independent approaches (including metagenomics). A total of 24 phyla and 460 genera were identified, with predominance of Gammaproteobacteria in all samples. Pathogenic genera corresponded to 8.6% of all sequences on average, mainly Acinetobacter and Streptococcus. Significant differences (p < 0.05) in the proportion of pathogens were observed between APT and the other samples, suggesting that the secondary treatment reduced its abundance. The MAS achieved 64.0-99.7% average removal efficiency for total (THB) and resistant heterotrophic bacteria, although the proportions of ARB/THB have increased for sulfamethoxazole, cephalexin, ciprofloxacin, and tetracycline. A total of 10 7 copies/mL of intI1 gene remained in the final effluent, suggesting that the treatment did not significantly remove this gene and possibly other ARGs. In accordance, metagenomic results suggested that number of reads recruited to plasmid-associated ARGs became more abundant in the pool throughout the treatment, suggesting that it affected more the bacteria without these ARGs than those with it. In conclusion, disinfected effluents are still a potential source for ARB and ARGs, which highlights the importance to investigate ways to mitigate their release into the environment., (© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

Medical overuse-defined as the provision of health services for which potential harms exceed potential benefits-constitutes a paradigm of low-value care and is seen as a threat to the quality of care. Value in healthcare implies a precise definition of disease. However, defining a disease may not be straightforward since clinical data do not show discrete boundaries, calling for some clinical judgment. And, if in time a redefinition of disease is needed, it is important to recognize that it can induce overdiagnosis, the identification of medical conditions that would, otherwise, never cause any significant symptoms or lead to clinical harm. A classic example is the impact of recommendations from professional societies in the late 1990s, lowering the threshold for abnormal total cholesterol from 240 mg/dl to 200 mg/dl. Due to these changes in risk factor definition, literally overnight there were 42 million new cases eligible for treatment in the United States. The same happened with hypertension-using either the 2019 NICE guidelines or the 2018 ESC/ECC guidelines criteria for arterial hypertension, the proportion of people overdiagnosed with hypertension was calculated to be between 14% and 33%. In this review, we will start by discussing resource overuse. We then present the basis for disease definition and its conceptual problems. Finally, we will discuss the impact of changing risk factor/disease definitions in the prevalence of disease and its consequences in overdiagnosis and overtreatment (a problem particularly relevant when definitions are widened to include earlier or milder disease)., (© 2022 The Authors. Journal of Internal Medicine published by John Wiley & Sons Ltd on behalf of Association for Publication of The Journal of Internal Medicine.)

The use of the anammox process for nitrogen removal has gained popularity across the world due to its low energy consumption and waste generation. Anammox reactors have been used to treat ammonium-rich effluents such as chemical, pharmaceutical, semiconductor, livestock, and coke oven wastewater. Recently, full-scale installations have been implemented for municipal wastewater treatment. The efficiency of biological processes is susceptible to inhibitory effects of pollutants present in wastewater. Considering the increasing number of emerging contaminants detected in wastewater, the impacts of the different types of pollutants on anammox bacteria must be understood. This review presents a compilation of the studies assessing the inhibitory effects of different wastewater pollutants towards anammox activity. The pollutants were classified as antibiotics, aromatics, azoles, surfactants, microplastics, organic solvents, humic substances, biodegradable organic matter, or metals and metallic nanoparticles. The interactions between the pollutants and anammox bacteria have been described, as well as the interactions between different pollutants leading to synergistic effects. We also reviewed the effects of pollutants on distinct species of anammox bacteria, and the main toxicity mechanisms leading to irreversible loss of anammox activity have been identified. Finally, we provided an analysis of strategies to overcome the inhibitory effects of wastewater pollutants on the nitrogen removal performance. We believe this review will contribute with essential information to assist the operation and design of anammox reactors treating different types of wastewaters., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2021 Elsevier B.V. All rights reserved.)

Many regions around the globe are subjected to precipitation-data scarcity that often hinders the capacity of hydrological modeling. The entropy theory and the principle of maximum entropy can help hydrologists to extract useful information from the scarce data available. In this work, we propose a new method to assess sub-daily precipitation features such as duration and intensity based on daily precipitation using the principle of maximum entropy. Particularly in arid and semiarid regions, such sub-daily features are of central importance for modeling sediment transport and deposition. The obtained features were used as input to the SYPoME model (sediment yield using the principle of maximum entropy). The combined method was implemented in seven catchments in Northeast Brazil with drainage areas ranging from 10 -3 to 10 +2 km 2 in assessing sediment yield and delivery ratio. The results show significant improvement when compared with conventional deterministic modeling, with Nash-Sutcliffe efficiency (NSE) of 0.96 and absolute error of 21% for our method against NSE of -4.49 and absolute error of 105% for the deterministic approach.

Evaporation is a major factor controlling the hydrological dynamics of surface water reservoirs in dry environments, therefore quantification with minimal uncertainties is desired. The aim of this paper is to assess the spatial variability and impact of riparian vegetation on reservoir evaporation by remote sensing. Eight reservoirs located in subhumid and semi-arid climates in the Brazilian Drylands were studied. Scenes from Landsat 5 and Landsat 8 satellites (1985 and 2018) supplied the data for four evaporation models. For reference evaporation, the Class A Pan and Piché Evaporimeter closest to the reservoirs were considered. The occurrence/density of riparian vegetation was associated with the Normalized Difference Vegetation Index (NDVI) and its influence on evaporation was assessed. The Surface Energy Balance System for Water (AquaSEBS) model presented the best average performance (Nash-Sutcliffe Efficiency coefficient 0.40 ± 0.19). Evaporation was observed to be higher at the reservoirs' margins and near the dams, due to the contact of exposed soil and rock/concrete, respectively, which transfer heat to the water. Marginal areas near the riparian forest presented low evaporation rates with decreases between 18% and 31% in relation to the average. This interdependence was evidenced by the high negative correlation (R 2 0.87-0.96) between NDVI and evaporation; vegetation reduces radiation because of the shading of the reservoir margin and changes local aerodynamics, reducing evaporation. Depending on the spatial variability of evaporation, it was found that the volumes transferred to the atmosphere may have variations of up to 30%. On average, the evaporated volume in all the studied reservoirs is 450,000 m 3 /day, a quantity enough to supply more than two million people. Overall, the results of this study contribute not only to a better understanding of the spatial variability of evaporation in surface reservoirs, but also of the interdependence between riparian vegetation and evaporation rates., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2021 Elsevier B.V. All rights reserved.)

The multiple-year drought that started in 2011 and reached climax in 2015 was the most severe and prolonged one in the semiarid northeastern (NE) Brazil in recent decades. This study aimed to investigate the reservoir surface water volume (SWV) variation in NE Brazil from 2009 to 2017 in four representative regions covering a total area of approximately 10,000 km 2 there and encompassing 2,140 reservoirs (areas range from 0.003 to 21 km 2 ). High-resolution (10 m) digital elevation models (DEMs) were generated from the TanDEM-X data acquired during October-December 2015 to represent the reservoirs' bathymetric maps. The water extents in the reservoirs were delineated from high-resolution (6.5 m) RapidEye images acquired during 2009-2017. The combination of the aforementioned two variables yielded reservoir SWV with an accuracy of 0.64 × 10 6 -1.06 × 10 6 m 3 , corresponding to 3.1%-5.6% of the maximum SWV in the reservoirs. The results showed that: 1) 81%-99% of the reservoirs in the four regions were from the groups with maximum water extent <50 ha and contributed 2%-59% of the regional reservoir SWV. In contrast, 0.6%-20% of the reservoirs were from the group of >50 ha and contributed 40%-98% to the regional SWV; 2) From 2009 to 2017, reservoir SWV in the four regions decreased at the rates of 2.3 × 10 6 -17.8 × 10 6 m 3 /year; and 3) The SWV in the reservoirs responded differently to the regional terrestrial water budget, i.e. the differences between precipitation and evapotranspiration (P-ET). This study filled the data gap of bathymetric maps for the 2140 reservoirs, regardless of their sizes and macrophyte coverage. The SWV variations derived in those reservoirs over a period covering the recent drought can support better preparedness for drought in NE Brazil and better understanding of the regional hydrology in semi-arid regions., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2021 Elsevier B.V. All rights reserved.)

Nitrification is a biochemical process that allows oxidation of ammonium ion to nitrite, and nitrite to nitrate in a system. Aerobic processes, such as use of submerged biological aerated filter (SBAF), enable nitrification. However, some variables that are entirely unavailable or not available at the required concentration range may hamper the process. In this study, nitratation under high dissolved oxygen (DO) concentrations was evaluated in laboratory-scale bioreactors containing 10% inoculum (0.5 kg kg -1 ) fed with affluent from a SBAF that receive the sewage generated from washing the bays of a dog kennel. The following variables were monitored over time: ammoniacal nitrogen (12.44-29.62 mg L -1 ), nitrite (0.28-0.54 mg L -1 ), nitrate (1.75-3.55 mg L -1 ), pH (8.11 ± 0.62), temperature (21.61 ± 1.24°C) and DO (9.69 ± 0.36 mg L -1 ). Quantification of nitrifying bacteria by the multiple tube technique showed the value of 1.4 × 10 12 MPN mL -1 for ammonia-oxidizing bacteria (AOB) and 9.2 × 10 14 MPN mL -1 for nitrite-oxidizing bacteria. These values were higher than those found in a synthetic medium, which can be explained by the greater availability of ammonium and nitrite in the effluent. By the extraction of genomic DNA, and PCR, with specific primers, the presence of the AmoA (Ammonia monooxygenase) gene for AOB and of the Nitrobacter was detected in the bioreactor samples. By PCR-DGGE, the sequenced bands showed high similarity with denitrifying bacteria, such as Pseudomonas , Limnobacter , Thauera , Rhodococcus, and Thiobacillus . Thus, the saturation of dissolved oxygen in the system resulted in improvement in the nitratation step and allowed detection of bacterial genera involved in the process.

Brazil has become one of the epicentres of the COVID-19 pandemic, with cases heavily concentrated in large cities. Testing data is extremely limited and unreliable, which restricts health authorities' ability to deal with the pandemic. Given the stark demographic, social and economic heterogeneities within Brazilian cities, it is important to identify hotspots so that the limited resources available can have the greatest impact. This study shows that decentralised monitoring of SARS-CoV-2 RNA in sewage can be used to assess the distribution of COVID-19 prevalence in the city. The methodology developed in this study allowed the identification of hotspots by comprehensively monitoring sewers distributed through Belo Horizonte, Brazil's third largest city. Our results show that the most vulnerable neighbourhoods in the city were the hardest hit by the pandemic, indicating that, for many Brazilians, the situation is much worse than reported by official figures., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

In recent years, an unprecedented increase in the development of products and technologies to protect the human being has been observed. Now, more than ever, the world population is exposed to several threats, harmful to their well-being and health. Chemical and biological hazardous agents stand out as one of the biggest threats, not only for the military forces, but also for the civilians. Consequently, it's essential to develop personal protective systems that are able to protect their user, not only passively, but actively, being able to detect, adsorb, degrade and decontaminate pesticides, pollutants, microorganisms and most importantly: chemical/biological warfare agents. One recent strategy for the development of active fibrous structures with improved functions and new properties is their functionalization with nanoparticles (NPs), especially metal oxides. Although their known effectiveness in the decomposition of harmful agents, the NPs could also include other functionalities in the same structure using low quantities of material, without adding extra weight, which is of huge importance for a soldier in the battlefield. The use of natural fibers as the substrate is also very interesting, since this material is a much sustainable alternative when compared to synthetic ones, also providing excellent properties.

To ensure microbial activity and a reaction equilibrium with efficiency and energy saving, it is important to know the factors that influence microbiological nitrogen removal in wastewater. Thus, it was investigated the microorganisms and their products involved in the treatment of kennel effluents operated with different aeration times, phase 1 (7 h of continuous daily aeration), phase 2 (5 h of continuous daily aeration), and phase 3 (intermittent aeration every 2 h), monitoring chemical and physical parameters weekly, monthly microbiological, and qualitative and quantitative microbiological analyzes at the end of each applied aeration phase. The results showed a higher mean growth of nitrifying bacteria (NB) (10 6 ) and denitrifying bacteria (DB) (10 22 ) in phase with intermittent aeration, in which better total nitrogen (TN) removal performance, with 33%, was achieved, against 21% in phase 1 and 17% in phase 2, due to the longer aeration time and lower carbon/nitrogen ratio (15.7), compared with the other phases. The presence of ammonia-oxidizing bacteria (AOB), the genus Nitrobacter nitrite-oxidizing bacteria (NOB), and DB were detected by PCR with specific primers at all phases. The analysis performed by 16S-rRNA DGGE revealed the genres Thauera at all phases; Betaproteobacteria and Acidovorax in phase 3; Azoarcus in phases 2 and 3; Clostridium, Bacillus, Lactobacillus, Turicibacter, Rhodopseudomonas, and Saccharibacteria in phase 1, which are related to the nitrogen removal, most of them by denitrifying. It is concluded that, with the characterization of the microbial community and the analysis of nitrogen compounds, it was determined, consistently, that the studied treatment system has microbiological capacity to remove TN, with the phase 3 aeration strategy, by simultaneous nitrification and denitrification (SND). Due to the high density of DB, most of the nitrification occurred by heterotrophic nitrification-aerobic. And denitrification occurred by heterotrophic and autotrophic forms, since the higher rate of oxygen application did not harm the DB. Therefore, the aeration and carbon conditions in phase 3 favored the activity of the microorganisms involved in these different routes. It is considered that, in order to increase autotrophic nitrification-aerobic, it is necessary to exhaust the volume of sludge in the secondary settlers (SD), further reducing the carbon/nitrogen ratio, through more frequent cleaning, whose periodicity should be the object of further studies. Graphical abstract.

Although the widespread use of sewage sludge in developing countries is common, little is known about how sludge disposal can affect the microbial composition and diversity of tropical soils. We evaluated the effects of the sewage sludges of two types of anaerobic digestors differing, by the biological treatment they have undergone (uplow anaerobic sludge blanket and activated sludge digester), and two different disposal methods (surface and incorporated) on tropical soils. Samples were taken from topsoil (0-10 cm) and analyzed by amplifying the 16S rRNA genes to study the microbial community, and physicochemical analysis was performed concomitantly. The results indicated that, in general, sewage sludge amendment (SSA) significantly changed the tropical soil bacterial community by the sludge type and by application method. Moreover, the redundancy analysis diagram indicates that changes in soil chemical parameters over time due to SSA resulted in changes in the bacterial community's composition, increasing the population responsible for recycling nutrients in the soil.

In this research, the trade-off between the number of restrictions and the robustness of the primary formulation of entropy models was evaluated. The performance of six hydrodynamic models in open channels was assessed based on 1730 Laser-Doppler anemometry data. It was investigated whether it is better to use an entropy-based model with more restrictions and a weak primary formulation or a model with fewer restrictions, but with a strong formulation. In addition, it was also investigated whether the model performance improves with the insertion of restrictions. Three of the investigated models have a weak formulation (open-channel velocity field represented by Cartesian coordinates); while the other three models have a strong formulation, according to which isovels are represented by curvilinear coordinates. The results indicated that models with two restrictions performed better than those with one restriction, since the additional restriction includes information relevant to the system. Models with three restrictions perform worse than those with two restrictions, because the information lost due to the use of a numerical solution was more substantial than the information gained by the third restriction. In conclusion, a strong primary formulation brought more information to the system than the inclusion of a third constraint.

Graphene and its derivatives have shown outstanding potential in many fields and textile/composites industry are not an exception. Giving their extraordinary properties, Graphene Nanoplatelets (GNPs) are excellent candidates for providing new functionalities to fibers and composites. In this work, natural fabrics (flax) were functionalized with chitosan (CS) based polymeric formulations of GNPs to develop fibrous systems with electrical properties as well as other functionalities. One of the greatest disadvantages of using carbon-based materials for fabrics' impregnation is their difficult dispersion. Therefore, several polymers were used as matrices, binding and dispersive agents including chitosan, polyethylene glycol (PEG), and glycerol. All the systems were characterized using several techniques that demonstrated the presence and incorporation of the GNPs onto the composites. Besides their characterization, considering their use as smart materials for monitoring and sensing applications, electrical properties were also evaluated. The highest value obtained for electrical conductivity was 0.04 S m -1 using 2% of GNPs. Furthermore, piezoresistive behavior was observed with Gauge Factor (GF) of 1.89 using 0.5% GNPs. Additionally, UV (ultraviolet) protection ability and hydrophobicity were analyzed, confirming the multifunctional behavior of the developed systems extending their potential of application in several areas.

Two bioreactors were investigated as an alternative for the post-treatment of effluent from an upflow anaerobic sludge blanket (UASB) reactor treating domestic sewage, aiming at dissolved sulfide and methane removal. The bioreactors (R-control and R-air) were operated at different hydraulic retention times (HRT; 6 and 3 h) with or without aeration. Large sulfide and methane removal efficiencies were achieved by the microaerated reactor at HRT of 6 h. At this HRT, sulfide removal efficiencies were equal to 61% and 79%, and methane removal efficiencies were 31% and 55% for R-control and R-air, respectively. At an HRT of 3 h, sulfide removal efficiencies were 22% (R-control) and 33% (R-air) and methane removal did not occur. The complete oxidation of sulfide, with sulfate formation, prevailed in both phases and bioreactors. However, elemental sulfur formation was more predominant at an HRT of 6 h than at an HRT of 3 h. Taken together, the results show that post-treatment improved the anaerobic effluent quality in terms of chemical oxygen demand and solids removal. However, ammoniacal nitrogen was not removed due to either the low concentration of air provided or the absence of microorganisms involved in the nitrogen cycle.

Alkaline treatment is widely used to reduce pathogens in sewage sludge in developing countries and guarantee that it is safe for use in agriculture. The aim of this study was to investigate the effect of alkaline treatment applied to waste-activated (WAS) and Upflow Anaerobic Sludge Blanket (UASB)-sludge on the bacterial community, pathogens (viable helminths eggs and Salmonella spp), and antibiotic resistance genes (ARG). The bacterial community structure was examined through denaturing gel gradient electrophoresis (DGGE), targeting 16S rRNA genes. Polymerase chain reaction (PCR) was applied to evaluate the presence of several ARGs. The conducted alkaline experiment consisted of adding hydrated lime (Ca(OH) 2 ) to sewage sludges. Samples were taken before and after 2, 24, 48, and 72 hours of treatment. Alkaline treatment changed considerably the bacterial community structure and after 24 hours, shifts in bacterial profiles were more pronounced in the UASB sludge sample than in WAS . Some bacteria remained under extreme pH conditions (pH > 12), such as Azospira oryzae and Dechloromonas denitrificans in the WAS samples, and Geothrix and Geobacter in the UASB sludge samples. The values of pathogens and indicators in the sludge after 24 hours of alkaline treatment meet sanitary law regulations and thus the sludges could have the potential to agricultural distribution. It is important to highlight that ARG, which are not currently present in sanitary regulations, were detected in the sludge samples after the alkaline treatment, which could be a concern for human health.

The feasibility of employing anammox and partial nitritation-anammox (PN/A) processes for nitrogen removal from food waste (FW) digestate was investigated in this study. The effects of different aeration strategies on the microbial community were also investigated. To achieve this, after anammox enrichment (Phase 1), the reactor was fed with digestate supplemented with nitrite (Phase 2), and subsequently different aeration strategies were evaluated to establish PN/A. Aeration strategies with high anoxic periods (30 and 45 min) in relation to aerobic periods (15 min) coupled with low air flow rates (0.026 L  min -1 . L reator -1 ) were found to be better for establishing PN/A, as coefficients of produced nitrate/removed ammonium were closer to those reported previously (0.17 and 0.21). Aeration conditions considerably altered the microbial community. Candidatus Brocadia was replaced by Candidatus Jettenia, after the first aeration strategies. These results support the feasibility of FW digestate treatment using anammox and PN/A processes and provide a better understanding of the effect of aeration on microbial dynamics in PN/A reactors., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

This study aimed to assess the tissue composition and allometric growth of carcasses of Santa Inês and crossbred Dorper x Santa Inês lambs confined for different periods at three body weight classes. Sixty-four lambs from Santa Inês (SI) and crossbred Dorper x Santa Inês (DSI) were slaughtered. The carcasses were refrigerated at 4 °C for 24 h, and half of the right side carcass was divided into five primary cuts, which were then dissected into bone, muscle, and fat. The corrected cold carcass weight (CCWc) was calculated based on the sum of the cuts. There was no effect of the interactions evaluated (P > 0.05) among the factors tested. CCWc, muscle (kg), fat (kg), bone (%), M:F, M:B, F:B, and M+F:B were influenced by the lambs' genetic group (P < 0.05). An effect of body weight at the beginning of confinement was found only for CCWc, muscle (kg), fat (kg), bone (kg), bone (%), and M:F. The crossbreeding of Santa Inês with breeds Dorper promotes improvements in the qualitative characteristics of the carcass. To obtain better relationships of the quantitative characteristics of the carcass, of sheep introduced in confinements with larger weights, the slaughter weight must be adjusted to that initial weight.

In the present study, a multistage route is proposed for the treatment of biodiesel industry wastewater (BWW) containing around 1000 mg L -1 of total organic carbon (TOC), 3500 mg L -1 of chemical oxygen demand (COD), and 1325 mg L -1 of oil and grease. Initially, BWW aerobic biodegradability was assessed via Zhan-Wellens biodegradability test to confirm the appropriate treatment route. Then, a hybrid moving bed bioreactor (MBBR) system was chosen as the first treatment stage. The hybrid MBBR achieved 69 and 68% removal of COD and TOC removals, respectively, and provided great conditions for biomass growth. The bacterial community present in the hybrid MBBR was investigated by PCR-DGGE and potential biodegraders were identified such as: members of Desulfuromonadales, Nocardioidaceae and Pseudomonadaceae. Since biodegradation in the hybrid MBBR alone was unable to meet quality requirements, advanced oxidation processes, such as Fenton and photo-Fenton, were optimized for application as additional treatment stages. Physicochemical properties and acute toxicity of BWW were analyzed after the multistage routes: hybrid MBBR + Fenton, hybrid MBBR + photo-Fenton and hybrid MBBR + UV-C 254nm /H 2 O 2 . Hybrid MBBR + Fenton or photo-Fenton showed overall COD removal efficiencies greater than 95% and removed acute toxicity, thus being appropriate integrated routes for the treatment of real BWW. Graphical abstract ᅟ.

Two bioreactors were investigated as an alternative to post-treatment of effluent from an upflow anaerobic sludge blanket (UASB) reactor treating domestic sewage, with an aim of oxidizing sulfide into elemental sulfur, and removal of solid and organic material. The bioreactors were operated at different hydraulic retention times (HRTs) (6, 4, and 2 h) and in the presence or absence (control) of packing material (polypropylene rings). Greater sulfide removal efficiencies - 75% (control reactor) and 92% (packed reactor) - were achieved in both reactors for an HRT of 6 h. Higher organic matter (COD) and solid (TSS) removal levels were observed in the packed reactor, which produced effluent with low COD (100 mg CODL -1 ) and TSS concentrations (30 mg TSSL -1 ). Denaturing gradient gel electrophoresis results revealed that a metabolically diverse bacterial community was present in both bioreactors, with sequences related to heterotrophic bacteria, sulfur bacteria (Thiocapsa, Sulfurimonas sp., Chlorobaculum sp., Chromatiales and Sulfuricellales), phototrophic purple non-sulfur bacteria (Rhodopseudomonas, Rhodocyclus sp.) and cyanobacteria. The packed reactor presented higher extracellular sulfur formation and potential for elemental sulfur recovery was seen. Higher efficiencies related to the packed reactor were attributed to the presence of packing material and higher cell retention time. The studied bioreactors seemed to be a simple and low-cost alternative for the post-treatment of anaerobic effluent.

The effects of temperature reduction (from 35 °C to 20 °C) on nitrogen removal performance and microbial diversity of an anammox sequencing batch reactor were evaluated. The reactor was fed for 148 days with anaerobically pretreated municipal wastewater amended with nitrite. On average, removal efficiencies of ammonium and nitrite were high (96%) during the enrichment period and phases 1 (at 35 °C) and 2 (at 25 °C), and slightly decreased (to 90%) when the reactor was operated at 20 °C. Deep sequencing analysis revealed that microbial community structure changed with temperature decrease. Anammox bacteria (Ca. Brocadia and Ca. Anammoximicrobium) and denitrifiers (Burkholderiales, Myxococcales, Rhodocyclales, Xanthomonadales, and Pseudomonadales) were favoured when the temperature was lowered from 35 °C to 25 °C, while Anaerolineales and Clostridiales were negatively affected. The results support the feasibility of using the anammox process for mainstream nitrogen removal from anaerobically pretreated municipal wastewater at typical tropical temperatures., (Copyright © 2018 Elsevier Ltd. All rights reserved.)

Understanding latitudinal variation in avian life-history traits has been a focus of many demographic studies around the world. However, we still know little about annual or intra-annual demographic variation within tropical regions or about how factors such as breeding season and precipitation influence demographic rates. In this study, we estimated intra-annual apparent survival of the White-lined Tanager (Tachyphonus rufus) using capture-mark-recapture data from northeastern Brazil. We tested whether survival varied seasonally (breeding vs. non-breeding), with rainfall, by age and residence status in our study area. Intra-annual apparent survival was correlated with the reproductive cycle, being lower during the breeding (0.65 ± 0.16 SE) vs. the non-breeding season (0.97 ± 0.05 SE). The annual apparent survival (~0.6) was relatively low for a tropical species. In both years, we observed highest abundance in spring (November, 3.1-3.7 birds/ha) and lowest abundance in autumn-winter periods (May-August, 1.1-1.4 bird/ha). The low survival during the breeding season probably reflects the trade-off between survival and reproduction and the cost of reproduction. Our findings represent an advance in the understanding of the demography of tropical birds because we did not find a predicted high annual apparent survival, and we elucidated some aspects of intra-annual variation in survival. Further exploration of latitudinal variation in demographic traits, especially in diverse, but poorly known habitats is needed to fully vet and develop life history theories.

Zero-discharge constructed wetland environments are more prone to the accumulation of pollutants. The relationship between filter media and microbial communities in this type of environment is still poorly known. We conducted bench-scale studies of different filter media (polyurethane foam, blast-furnace slag, and loofah) in these systems by simulating the batch operation with light greywater for 433 days. Physicochemical and microbiological analyses (scanning electron microscopy and polymerase chain reaction electrophoresis denaturing gradient gel) were used. In all systems, anoxic environments prevailed. These environments were crucial for methanogenesis and sulfidogenesis processes, which are primarily responsible for organic material conversion. The chemical oxygen demand/sulfate (COD/SO 4 2- ) ratio was the limiting factor in the competition of microorganisms involved in these processes. This condition, combined with the neutral-alkaline pH, also allowed Chloroflexi phylum bacteria to oxidize sulfide to sulfate and elemental sulfur in all studied media. The results showed strong evidence supporting that the microbial community formed in the present study is more related to operational/environmental conditions than to the different tested filter media. Thus, this demonstrates that the control of interactive effects between pH, redox potential, and the COD/SO 4 2- ratio can prevent the accumulation and/or release of sulfide in anoxic environments.

Accumulated sludge in polishing (maturation) ponds reduces the hydraulic retention time (smaller useful volume), and this could potentially lead to a decrease in performance. However, settled biomass, present in the sediments, can contribute to nitrogen removal by different mechanisms such as nitrification and denitrification. This study investigated the influence of the bottom sludge present in a shallow maturation pond treating the effluent from an anaerobic reactor on the nitrification and denitrification processes. Nitrification and denitrification rates were determined in sediment cores by applying ammonia pulses. Environmental conditions in the medium were measured and bacteria detected and quantified by real-time polymerase chain reaction (real-time PCR). The pond showed daily cycles of mixing and stratification and most of the bacteria involved in nitrogen removal decreased in concentration from the upper to the lower part of the sludge layer. The results indicate that denitrifiers, nitrifiers and anammox bacteria coexisted in the sludge, and thus different metabolic pathways were involved in ammonium removal in the system. Therefore, the sediment contributed to nitrogen removal, even with a decrease in the hydraulic retention time in the pond due to the volume occupied by the sludge.

Methane is produced in anaerobic environments, such as reactors used to treat wastewaters, and can be consumed by methanotrophs. The composition and structure of a microbial community enriched from anaerobic sewage sludge under methane-oxidation condition coupled to denitrification were investigated. Denaturing gradient gel electrophoresis (DGGE) analysis retrieved sequences of Methylocaldum and Chloroflexi. Deep sequencing analysis revealed a complex community that changed over time and was affected by methane concentration. Methylocaldum (8.2%), Methylosinus (2.3%), Methylomonas (0.02%), Methylacidiphilales (0.45%), Nitrospirales (0.18%), and Methanosarcinales (0.3%) were detected. Despite denitrifying conditions provided, Nitrospirales and Methanosarcinales, known to perform anaerobic methane oxidation coupled to denitrification (DAMO) process, were in very low abundance. Results demonstrated that aerobic and anaerobic methanotrophs coexisted in the reactor together with heterotrophic microorganisms, suggesting that a diverse microbial community was important to sustain methanotrophic activity. The methanogenic sludge was a good inoculum to enrich methanotrophs, and cultivation conditions play a selective role in determining community composition.

Water scarcity in the dry season is a vital problem in dryland regions such as northeastern Brazil. Water supplies in these areas often come from numerous reservoirs of various sizes. However, inventory data for these reservoirs is often limited due to the expense and time required for their acquisition via field surveys, particularly in remote areas. Remote sensing techniques provide a valuable alternative to conventional reservoir bathymetric surveys for water resource management. In this study single pass TanDEM-X data acquired in bistatic mode were used to generate digital elevation models (DEMs) in the Madalena catchment, northeastern Brazil. Validation with differential global positioning system (DGPS) data from field measurements indicated an absolute elevation accuracy of approximately 1m for the TanDEM-X derived DEMs (TDX DEMs). The DEMs derived from TanDEM-X data acquired at low water levels show significant advantages over bathymetric maps derived from field survey, particularly with regard to coverage, evenly distributed measurements and replication of reservoir shape. Furthermore, by mapping the dry reservoir bottoms with TanDEM-X data, TDX DEMs are free of emergent and submerged macrophytes, independent of water depth (e.g. >10m), water quality and even weather conditions. Thus, the method is superior to other existing bathymetric mapping approaches, particularly for inland water bodies. The proposed approach relies on (nearly) dry reservoir conditions at times of image acquisition and is thus restricted to areas that show considerable water levels variations. However, comparisons between TDX DEM and the bathymetric map derived from field surveys show that the amount of water retained during the dry phase has only marginal impact on the total water volume derivation from TDX DEM. Overall, DEMs generated from bistatic TanDEM-X data acquired in low water periods constitute a useful and efficient data source for deriving reservoir bathymetry and show great potential in large scale application., (Copyright © 2016 Elsevier B.V. All rights reserved.)

The use of biofilters (working bed volume of 7.85 L) for the oxidation of CH4 at low concentrations (from 0.17%v/v to 3.63%v/v, typically in waste gas from anaerobic sewage treatment) was investigated and four empty bed residence times were tested (in min): 42.8, 29.5, 19.6, and 7.4. Mixtures of organic (composted leaves) and three non-organic materials (sponge-based material - SBM, blast furnace slag - BFS, and expanded vermiculite - ExpV) were used as packing media. Along 188 operational days after the steady state was reached (95 days for start-up), the CH4 mineralization decreased while the inlet loads gradually increased from 3.0 ± 0.8 gCH4 m(-3) h(-1) to 148.8 ± 4.4 gCH4 m(-3) h(-1). The biofilter packed with ExpV showed the best results, since the CH4 conversions decreased from 95.0 ± 5.0% to 12.7 ± 3.7% as a function of inlet concentration, compared to the other two biofilters (SBM and BFS) which showed CH4 conversions decreasing from 56.0 ± 5.4% to 3.5 ± 1.2% as a function of inlet concentration. The methanotrophic activity of biomass taken from ExpV biofilter was three times higher than the activity of biomass from the other two biofilters. Taken together, these results suggested that ExpV provides an attractive environment for microbial growth, besides the mechanical resistance provided to the whole packing media, showing the potential to its use in biofiltration of diffuse CH4 emissions., (Copyright © 2016 Elsevier Ltd. All rights reserved.)

Background: Renal transplantation is the treatment of choice for patients with stage V chronic kidney disease, which does not have contraindications to the procedure and is more cost-effective than dialysis treatments and provides better survival and quality of life., Objective: The objective of this study was to evaluate the incidence of postoperative complications in kidney transplant recipients in a reference hospital., Methodology: This was a descriptive and retrospective study involving the analysis of patient records during hospitalization and outpatient treatment. We analyzed the demographics, clinical indicators, surgical techniques, and postoperative complications., Results: In the analysis of 147 transplantations, there was a higher incidence of transplantation in female recipients, average age of 37 years with a predominance of cadaveric transplantation. Of all pretransplantation comorbidities, hypertension was the most frequent. The overall incidence of surgical complications was 29.9%, with an incidence of vascular complications of 12.7%, 13.4% of surgical site complications, 8.2% of urologic complications, and 3% of hemorrhagic complications., Discussion: Vascular complications are serious complications and are associated with increased risk of graft loss (relative risk, 8.4), particularly arterial thrombosis. Patients with ureteral anastomosis using Lich-Gregoir technique showed lower urologic complications compared with patients with anastomosis by Leadbetter-Politano technique., Conclusion: Surgical complications have different clinical effects, depending on their category. The vascular complications are associated with graft lost., (Copyright © 2016 Elsevier Inc. All rights reserved.)

Long-term effects of COD/N ratios on the nitrogen removal performance and bacterial community of an anammox reactor were evaluated by adding a synthetic medium (with glucose) and real anaerobic effluent to a SBR. At a COD/N ratio of 2.8 (COD, 390mg·L(-1)) ammonium removal efficiency was 66%, while nitrite removal remained high (99%). However, at a COD/N ratio of 5.0 (COD, 300mg·L(-1)), ammonium and nitrite removal efficiencies were high (84% and 99%, respectively). High COD, nitrite, and ammonium removal efficiencies (80%, 90% and 95%, respectively) were obtained on adding anaerobically pre-treated municipal wastewater (with nitrite) to the reactor. DGGE revealed that the addition of anaerobic effluent changed the bacterial community structure and selected for DNA sequences related to Brocadia sinica and Chloroflexi. Adding glucose and anaerobic effluent increased denitrifiers concentration threefold. Thus, the possibility of using the anammox process to remove nitrogen from anaerobically pre-treated municipal wastewater was demonstrated., (Copyright © 2016 Elsevier Ltd. All rights reserved.)

We investigated the response of microbial community to changes in H2S loading rate in a microaerated desulphurisation system treating biogas from vinasse methanisation. H2S removal efficiency was high, and both COD and DO seemed to be important parameters to biomass activity. DGGE analysis retrieved sequences of sulphide-oxidising bacteria (SOB), such as Thioalkalimicrobium sp. Deep sequencing analysis revealed that the microbial community was complex and remained constant throughout the experiment. Most sequences belonged to Firmicutes and Proteobacteria, and, to a lesser extent, Bacteroidetes, Chloroflexi, and Synergistetes. Despite the high sulphide removal efficiency, the abundance of the taxa of SOB was low, and was negatively affected by the high sulphide loading rate., (Copyright © 2016 Elsevier Ltd. All rights reserved.)

The ethyl acetate extract from the fruit pulp of Caryocar coriaceum Wittm (Caryocaraceae), popularly known as pequi, has wide applications in popular medicine. Preclinical tests have demonstrated the therapeutic properties of the oil. We investigated the antinociceptive and anti-inflammatory effects of Pequi C. coriaceum Wittm ethyl acetate extract (PCCO) on zymosan-induced arthritis in rat knee joint. The animals were pretreated with PCCO for 7 consecutive days or with a single dose. Paw elevation time (PET), leukocyte infiltration, myeloperoxidase activity (MPO) and cytokine levels were assessed 4h after zymosan injection. Synovial tissue was harvested for immunohistochemical analysis, edema and vascular permeability. We observed a significant decrease in PET with PCCO pretreatment. PCCO showed a significant reduction of leukocyte migration and a decrease in MPO. Decreases were observed in cytokine release in the synovial fluid and TNF-α and cyclooxygenase-1 immunostaining in synovial tissue. Edema was inhibited by treatment with all doses of PCCO. The data suggest that PCCO exerts antinociceptive and anti-inflammatory effects on arthritis in rats., (Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.)

The endocrine cells (ECs) of the gastrointestinal mucosa form the largest endocrine system in the body, not only in terms of cell numbers but also in terms of the different produced substances. Data describing the association between the relative distributions of the peptide-specific ECs in relation to feeding habits can be useful tools that enable the creation of a general expected pattern of EC distribution. We aimed to investigate the distribution of ECs immunoreactive for the peptides gastrin (GAS), cholecystokinin (CCK-8), neuropeptide Y (NPY), and calcitonin gene-related peptide (CGRP) in different segments of the digestive tract of carnivorous fish dorado (Salminus brasiliensis) by using immunohistochemistry procedures. The distribution of endocrine cells immunoreactive for gastrin (GAS), cholecystokinin (CCK-8), neuropeptide Y (NPY), and calcitonin gene-related peptide (CGRP) in digestive tract of dorado S. brasiliensis was examined by immunohistochemistry. The results describe the association between the distribution of the peptide-specific endocrine cells and feeding habits in different carnivorous fish. The largest number of endocrine cells immunoreactive for GAS, CCK-8, and CGRP were found in the pyloric stomach region and the pyloric caeca. However, NPY-immunoreactive endocrine cells were markedly restricted to the midgut. The distribution pattern of endocrine cells identified in S. brasiliensis is similar to that found in other carnivorous fishes., (Copyright © 2015 Elsevier Ltd. All rights reserved.)

The bacterial community composition of a down-flow sponge-based trickling filter treating upflow anaerobic sludge blanket (UASB) effluent was investigated by pyrosequencing. Bacterial community composition considerably changed along the reactor and over the operational period. The dominant phyla detected were Proteobacteria, Verrucomicrobia, and Planctomycetes. The abundance of denitrifiers decreased from the top to the bottom and it was consistent with the organic matter concentration gradients. At lower loadings (organic and nitrogen loading rates), the abundance of anammox bacteria was higher than that of the ammonium-oxidizing bacteria in the upper portion of the reactor, suggesting that aerobic and anaerobic ammonium oxidation occurred. Nitrification occurred in all the compartments, while anammox bacteria prominently appeared even in the presence of high organic carbon to ammonia ratios (around 1.0-2.0 gCOD gN(-1)). The results suggest that denitrifiers, nitrifiers, and anammox bacteria coexisted in the reactor; thus, different metabolic pathways were involved in ammonium removal in the post-UASB reactor sponge-based.

The effects of phenol on the nitrogen removal performance of a sequencing batch reactor (SBR) with anammox activity and on the microbial community within the reactor were evaluated. A phenol concentration of 300 mg L(-1) reduced the ammonium-nitrogen removal efficiency of the SBR from 96.5% to 47%. The addition of phenol changed the microbial community structure and composition considerably, as shown by denaturing gradient gel electrophoresis and 454 pyrosequencing of 16S rRNA genes. Some phyla, such as Proteobacteria, Verrucomicrobia, and Firmicutes, increased in abundance, whereas others, such as Acidobacteria, Chloroflexi, Planctomycetes, GN04, WS3, and NKB19, decreased. The diversity of the anammox bacteria was also affected by phenol: sequences related to Candidatus Brocadia fulgida were no longer detected, whereas sequences related to Ca. Brocadia sp. 40 and Ca. Jettenia asiatica persisted. These results indicate that phenol adversely affects anammox metabolism and changes the bacterial community within the anammox reactor., (Copyright © 2014 Elsevier Ltd. All rights reserved.)