57 results on '"Araújo, ME"'
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2. Rationale, study design, and analysis plan of the Alveolar Recruitment for ARDS Trial (ART): study protocol for a randomized controlled trial
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Cavalcanti, AB, Berwanger, O, Suzumura, ÉA, Amato, MB, Tallo, FS, Rezende, AC, Telles, MM, Romano, E, Guimarães, HP, Regenga, MM, Takahashi, LN, Oliveira, RP, Carvalho, VO, Díaz Quijano, FA, Carvalho, CR, Kodama, AA, Ribeiro, GF, Abreu, MO, Oliveira, IM, Guyatt, G, Ferguson, N, Walter, S, Vasconcelos, MO, Segundo, VJ, Ferraz, ÍL, Silva, RS, de Oliveira Filho, W, Silva, NB, Heirel, C, Takatani, RR, Neto, JA, Neto, JC, Almeida, SD, Chamy, G, Neto, GJ, Dias, AP, Silva, RR, Tavares, RC, Souza, ML, Decio, JC, Lima, CM, Neto, FF, Oliveira, KR, Dias, PP, Brandão, AL, Ramos, JE Jr, Vasconcelos, PT, Flôres, DG, Filho, GR, Andrade, IG, Martinez, A, França, GG, Monteiro, LL, Correia, EI, Ribeiro, W, Pereira, AJ, Andrade, W, Leite, PA, Feto, JE, Holanda, MA, Amorim, FF, Margalho, SB, Domingues, SM Jr, Ferreira, CS, Ferreira, CM, Rabelo, LA, Duarte, JN, Lima, FB, Kawaguchi, IA, Maia, MO, Correa, FG, Ribeiro, RA, Caser, E, Moreira, CL, Marcilino, A, Falcão, JG, Jesus, KR, Tcherniakovisk, L, Dutra, VG, Thompson, MM, Piras, C, Giuberti, J. Jr, Silva, AS, Santos, JR, Potratz, JL, Paula, LN, Bozi, GG, Gomes, BC, Vassallo, PF, Rocha, E, Lima, MH, Ferreira, A. F, Gonçalves, F, Pereira, SA, Nobrega, MS, Caixeta, CR, Moraes, AP, Carvalho, AG, Alves, JD, Carvalho, FB, Moreira, FB, Starling, CM, Couto, WA, Bitencourt, WS, Silva, SG, Felizardo, LR, Nascimento, FJ, Santos, D, Zanta, CC, Martins, MF, Naves, SA, Silva, FD, Laube, G. Jr, Galvão, EL, Sousa, MF, Souza, MM, Carvalho, FL, Bergo, RR, Rezende, CM, Tamazato, EY, Sarat, SC Jr, Almeida, PS, Gorski, AG, Matsui, M, Neto, EE, Nomoto, SH, Lima, ZB, Inagaki, AS, Gil, FS, Araújo, MF, Oliveira, AE, Correa, TA, Mendonça, A, Reis, H, Carneiro, SR, Rego, LR, Cunha, AF, Barra, WF, Carneiro, M, Batista, RA, Zoghbi, KK, Machado, NJ, Ferreira, R, Apoena, P, Leão, RM, Martins, ER, Oliveira, ME, Odir, I, Kleber, W, Tavares, D, Araújo, ME, Brilhante, YN, Tavares, DC, Carvalho, WL, Winveler, GF, Filho, AC, Cavalcanti, RA, Grion, CM, Reis, AT, Festti, J, Gimenez, FM, Larangeira, AS, Cardoso, LT, Mezzaroba, TS, Kauss, IA, Duarte, PA, Tozo, TC, Peliser, P, Germano, A, Gurgel, SJ, Silva, SR, Kuroda, CM, Herek, A, Yamada, SS, Schiavetto, PM, Wysocki, N, Matsubara, RR, Sales, JA Jr, Laprovita, MP, Pena, FM, Sá, A, Vianna, A, Verdeal, JC, Martins, GA, Salgado, DR, Coelho, AM, Coelho, M, Morong, AS, Poquiriqui, RM, Ferreira, AP, Lucena, DN, Marino, NF, Moreira, MA, Uratani, CC, Severino, MA, Silva, PN, Medeiros, LG, Filho, FG, Guimarães, DM, Rezende, VM, Carbonell, RC, Trindade, RS, Pellegrini, JA, Boniatti, MM, Santos, MC, Boldo, R, Oliveira, VM, Corrêa, VM, Nedel, W, Teixeira, C, Schaich, F, Tagliari, L, Savi, A, Schulz, LF, Maccari, JG, Seeger, GM, Foernges, RB, Rieder, MM, Becker, DA, Broilo, FP, Schwarz, P, Alencastro, A, Berto, P, Backes, F, Dias, FS, Blattner, C, Martins, ET, Scaglia, NC, Vieira, SR, Prado, KF, Fialkow, L, Franke, C, Vieira, DF, Moraes, RB, Marques, LS, Hopf, JL, Wawrzeniak, IC, Rech, TH, Albuquerque, RB, Guerreiro, MO, Teixeira, LO, Macedo, PL, Bainy, MP, Ferreira, EV, Martins, MA, Andrade, LA, Machado, FO, Burigo, AC, Pincelli, M, Kretzer, L, Maia, IS, Cordeiro, RB, Westphal, G, Cramer, AS, Dadam, MM, Barbosa, PO, Caldeira, M, Brilenger, CO, Horner, MB, Oliveira, GL, Germiniani, BC, Duarte, R, Assef, MG, Rosso, D, Bigolin, R, Vanzuita, R, Prado, LF, Oliveira, V, Reis, DL, Morais, MO, Bastos, RS, Santana, HS, Silva, AO, Cacau, LA, Almeida, MS, Canavessi, HS, Nogueira, EE, Pavia, CL, Araujo, JF, Lira, JA, Nienstedt, EC, Smith, TC, Romano, M, Barros D, Costa, AF, Takahashi, L, Werneck, V, Farran, J, Henriques, LA, Miura, C, Lopes, RD, Vendrame, LS, Sandri, P, Galassi, MS, Amato, P, Toufen, C. Jr, Santiago, RR, Hirota, AS, Park, M, Azevedo, LC, Malbouison, LM, Costa, MC, Taniguchi, L, Pompílio, CE, Baruzzi, C, Andrade, AH, Taira, EE, Taino, B, Oliveira, CS, Silva, AC, Ísola, A, Rezende, E, Rodrigues, RG, Rangel, VP, Luzzi, S, Giacomassi, IW, Nassar, AP Jr, Souza, AR, Rahal, L, Nunes, AL, Giannini, F, Menescal, B, Morais, JE, Toledo, D, Morsch, RD, Merluzzi, T, Amorim, DS, Bastos, AC, Santos, PL, Silva, SF, Gallego, RC, Santos, GD, Tucci, M, Costa, RT, Santos, LS, Demarzo, SE, Schettino, GP, Suzuki, VC, Patrocinio, AC, Martins, ML, Passos, DB, Cappi, SB, Gonçalves, I. Jr, Borges, MC, Lovato, W, Tavares, MV, Morales, D, Machado, LA, Torres, FC, Gomes, TM, Cerantola, RB, Góis, A, Marraccini, T, Margarida, K, Cavalcante, E, Machado, FR, Mazza, BF, Santana, HB, Mendez, VM, Xavier, PA, Rabelo, MV, Schievano, FR, Pinto, WA, Francisco, RS, Ferreira, EM, Silva, DC, Arduini, RG, Aldrighi, JR, Amaro, AF, Conde, KA, Pereira, CA, Tarkieltaub, E, Oliver, WR, Guadalupe, EG, Acerbi, PS, Tomizuka, CI, Oliveira, TA, Geha, NN, Mecatti, GC, Piovesan, MZ, Salomão, MC, Moreno, MS, Orsatti, VN, Miranda, W, Ray, A, Guerra, A, Filho, ML, Ferreira, FH Jr, Filho, EV, Canzi, RA, Giuberti, AF, Garcez, MC, Sala, AD, Suguitani, EO, Kazue, P, Oliveira, LR, Infantini, RM, Carvalho, FR, Andrade, LC, Santos, TM, Carmona, CV, Figueiredo, LC, Falcão, A, Dragosavak, D, Filho, WN, Lunardi, MC, Lago, R, Gatti, C, Chiasso, TM, Santos, GO, Araujo, AC, Ornellas, IB, Vieira, VM, Hajjar, LA, Figueiredo, AC, Damasceno, B, Hinestrosa, A, Diaz Quijano, FA, CORTEGIANI, Andrea, RAINERI, Santi Maurizio, Cavalcanti, AB, Berwanger, O, Suzumura, ÉA, Amato, MB, Tallo, FS, Rezende, AC, Telles, MM, Romano, E, Guimarães, HP, Regenga, MM, Takahashi, LN, Oliveira, RP, Carvalho, VO, Díaz-Quijano, FA, Carvalho, CR, Kodama, AA, Ribeiro, GF, Abreu, MO, Oliveira, IM, Guyatt, G, Ferguson, N, Walter, S, Vasconcelos, MO, Segundo, VJ, Ferraz, ÍL, Silva, RS, de Oliveira Filho, W, Silva, NB, Heirel, C, Takatani, RR, Neto, JA, Neto, JC, Almeida, SD, Chamy, G, Neto, GJ, Dias, AP, Silva, RR, Tavares, RC, Souza, ML, Decio, JC, Lima, CM, Neto, FF, Oliveira, KR, Dias, PP, Brandão, AL, Ramos, JE Jr, Vasconcelos, PT, Flôres, DG, Filho, GR, Andrade, IG, Martinez, A, França, GG, Monteiro, LL, Correia, EI, Ribeiro, W, Pereira, AJ, Andrade, W, Leite, PA, Feto, JE, Holanda, MA, Amorim, FF, Margalho, SB, Domingues, SM Jr, Ferreira, CS, Ferreira, CM, Rabelo, LA, Duarte, JN, Lima, FB, Kawaguchi, IA, Maia, MO, Correa, FG, Ribeiro, RA, Caser, E, Moreira, CL, Marcilino, A, Falcão, JG, Jesus, KR, Tcherniakovisk, L, Dutra, VG, Thompson, MM, Piras, C, Giuberti, J Jr, Silva, AS, Santos, JR, Potratz, JL, Paula, LN, Bozi, GG, Gomes, BC, Vassallo, PF, Rocha, E, Lima, MH, Ferreira, A F, Gonçalves, F, Pereira, SA, Nobrega, MS, Caixeta, CR, Moraes, AP, Carvalho, AG, Alves, JD, Carvalho, FB, Moreira, FB, Starling, CM, Couto, WA, Bitencourt, WS, Silva, SG, Felizardo, LR, Nascimento, FJ, Santos, D, Zanta, CC, Martins, MF, Naves, SA, Silva, FD, Laube, G Jr, Galvão, EL, Sousa, MF, Souza, MM, Carvalho, FL, Bergo, RR, Rezende, CM, Tamazato, EY, Sarat, SC Jr, Almeida, PS, Gorski, AG, Matsui, M, Neto, EE, Nomoto, SH, Lima, ZB, Inagaki, AS, Gil, FS, Araújo, MF, Oliveira, AE, Correa, TA, Mendonça, A, Reis, H, Carneiro, SR, Rego, LR, Cunha, AF, Barra, WF, Carneiro, M, Batista, RA, Zoghbi, KK, Machado, NJ, Ferreira, R, Apoena, P, Leão, RM, Martins, ER, Oliveira, ME, Odir, I, Kleber, W, Tavares, D, Araújo, ME, Brilhante, YN, Tavares, DC, Carvalho, WL, Winveler, GF, Filho, AC, Cavalcanti, RA, Grion, CM, Reis, AT, Festti, J, Gimenez, FM, Larangeira, AS, Cardoso, LT, Mezzaroba, TS, Kauss, IA, Duarte, PA, Tozo, TC, Peliser, P, Germano, A, Gurgel, SJ, Silva, SR, Kuroda, CM, Herek, A, Yamada, SS, Schiavetto, PM, Wysocki, N, Matsubara, RR, Sales, JA Jr, Laprovita, MP, Pena, FM, Sá, A, Vianna, A, Verdeal, JC, Martins, GA, Salgado, DR, Coelho, AM, Coelho, M, Morong, AS, Poquiriqui, RM, Ferreira, AP, Lucena, DN, Marino, NF, Moreira, MA, Uratani, CC, Severino, MA, Silva, PN, Medeiros, LG, Filho, FG, Guimarães, DM, Rezende, VM, Carbonell, RC, Trindade, RS, Pellegrini, JA, Boniatti, MM, Santos, MC, Boldo, R, Oliveira, VM, Corrêa, VM, Nedel, W, Teixeira, C, Schaich, F, Tagliari, L, Savi, A, Schulz, LF, Maccari, JG, Seeger, GM, Foernges, RB, Rieder, MM, Becker, DA, Broilo, FP, Schwarz, P, Alencastro, A, Berto, P, Backes, F, Dias, FS, Blattner, C, Martins, ET, Scaglia, NC, Vieira, SR, Prado, KF, Fialkow, L, Franke, C, Vieira, DF, Moraes, RB, Marques, LS, Hopf, JL, Wawrzeniak, IC, Rech, TH, Albuquerque, RB, Guerreiro, MO, Teixeira, LO, Macedo, PL, Bainy, MP, Ferreira, EV, Martins, MA, Andrade, LA, Machado, FO, Burigo, AC, Pincelli, M, Kretzer, L, Maia, IS, Cordeiro, RB, Westphal, G, Cramer, AS, Dadam, MM, Barbosa, PO, Caldeira, M, Brilenger, CO, Horner, MB, Oliveira, GL, Germiniani, BC, Duarte, R, Assef, MG, Rosso, D, Bigolin, R, Vanzuita, R, Prado, LF, Oliveira, V, Reis, DL, Morais, MO, Bastos, RS, Santana, HS, Silva, AO, Cacau, LA, Almeida, MS, Canavessi, HS, Nogueira, EE, Pavia, CL, Araujo, JF, Lira, JA, Nienstedt, EC, Smith, TC, Romano, M, Barros D, Costa, AF, Takahashi, L, Werneck, V, Farran, J, Henriques, LA, Miura, C, Lopes, RD, Vendrame, LS, Sandri, P, Galassi, MS, Amato, P, Toufen, C Jr, Santiago, RR, Hirota, AS, Park, M, Azevedo, LC, Malbouison, LM, Costa, MC, Taniguchi, L, Pompílio, CE, Baruzzi, C, Andrade, AH, Taira, EE, Taino, B, Oliveira, CS, Silva, AC, Ísola, A, Rezende, E, Rodrigues, RG, Rangel, VP, Luzzi, S, Giacomassi, IW, Nassar, AP Jr, Souza, AR, Rahal, L, Nunes, AL, Giannini, F, Menescal, B, Morais, JE, Toledo, D, Morsch, RD, Merluzzi, T, Amorim, DS, Bastos, AC, Santos, PL, Silva, SF, Gallego, RC, Santos, GD, Tucci, M, Costa, RT, Santos, LS, Demarzo, SE, Schettino, GP, Suzuki, VC, Patrocinio, AC, Martins, ML, Passos, DB, Cappi, SB, Gonçalves, I Jr, Borges, MC, Lovato, W, Tavares, MV, Morales, D, Machado, LA, Torres, FC, Gomes, TM, Cerantola, RB, Góis, A, Marraccini, T, Margarida, K, Cavalcante, E, Machado, FR, Mazza, BF, Santana, HB, Mendez, VM, Xavier, PA, Rabelo, MV, Schievano, FR, Pinto, WA, Francisco, RS, Ferreira, EM, Silva, DC, Arduini, RG, Aldrighi, JR, Amaro, AF, Conde, KA, Pereira, CA, Tarkieltaub, E, Oliver, WR, Guadalupe, EG, Acerbi, PS, Tomizuka, CI, Oliveira, TA, Geha, NN, Mecatti, GC, Piovesan, MZ, Salomão, MC, Moreno, MS, Orsatti, VN, Miranda, W, Ray, A, Guerra, A, Filho, ML, Ferreira, FH Jr, Filho, EV, Canzi, RA, Giuberti, AF, Garcez, MC, Sala, AD, Suguitani, EO, Kazue, P, Oliveira, LR, Infantini, RM, Carvalho, FR, Andrade, LC, Santos, TM, Carmona, CV, Figueiredo, LC, Falcão, A, Dragosavak, D, Filho, WN, Lunardi, MC, Lago, R, Gatti, C, Chiasso, TM, Santos, GO, Araujo, AC, Ornellas, IB, Vieira, VM, Hajjar, LA, Figueiredo, AC, Damasceno, B, Hinestrosa, A, Diaz-Quijano, FA, Raineri, SM, and Cortegiani, A
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Research design ,ARDS ,medicine.medical_specialty ,Time Factors ,Ventilator-Induced Lung Injury ,Alveolar recruitment ,Treatment outcome ,Randomized ,Medicine (miscellaneous) ,Settore MED/41 - Anestesiologia ,Hospital mortality ,law.invention ,Positive-Pressure Respiration ,Study Protocol ,Mechanical ventilation ,Clinical trials ,Randomized controlled trial ,Clinical Protocols ,law ,Medicine ,Humans ,Pharmacology (medical) ,Hospital Mortality ,PEEP ,Protocol (science) ,Respiratory Distress Syndrome ,Acute respiratory distress syndrome ,business.industry ,respiratory system ,Length of Stay ,medicine.disease ,Clinical trial ,Pulmonary Alveoli ,Intensive Care Units ,Treatment Outcome ,Multicenter study ,Barotrauma ,Research Design ,Physical therapy ,business ,Brazil - Abstract
Background Acute respiratory distress syndrome (ARDS) is associated with high in-hospital mortality. Alveolar recruitment followed by ventilation at optimal titrated PEEP may reduce ventilator-induced lung injury and improve oxygenation in patients with ARDS, but the effects on mortality and other clinical outcomes remain unknown. This article reports the rationale, study design, and analysis plan of the Alveolar Recruitment for ARDS Trial (ART). Methods/Design ART is a pragmatic, multicenter, randomized (concealed), controlled trial, which aims to determine if maximum stepwise alveolar recruitment associated with PEEP titration is able to increase 28-day survival in patients with ARDS compared to conventional treatment (ARDSNet strategy). We will enroll adult patients with ARDS of less than 72 h duration. The intervention group will receive an alveolar recruitment maneuver, with stepwise increases of PEEP achieving 45 cmH2O and peak pressure of 60 cmH2O, followed by ventilation with optimal PEEP titrated according to the static compliance of the respiratory system. In the control group, mechanical ventilation will follow a conventional protocol (ARDSNet). In both groups, we will use controlled volume mode with low tidal volumes (4 to 6 mL/kg of predicted body weight) and targeting plateau pressure ≤30 cmH2O. The primary outcome is 28-day survival, and the secondary outcomes are: length of ICU stay; length of hospital stay; pneumothorax requiring chest tube during first 7 days; barotrauma during first 7 days; mechanical ventilation-free days from days 1 to 28; ICU, in-hospital, and 6-month survival. ART is an event-guided trial planned to last until 520 events (deaths within 28 days) are observed. These events allow detection of a hazard ratio of 0.75, with 90% power and two-tailed type I error of 5%. All analysis will follow the intention-to-treat principle. Discussion If the ART strategy with maximum recruitment and PEEP titration improves 28-day survival, this will represent a notable advance to the care of ARDS patients. Conversely, if the ART strategy is similar or inferior to the current evidence-based strategy (ARDSNet), this should also change current practice as many institutions routinely employ recruitment maneuvers and set PEEP levels according to some titration method. Trial registration ClinicalTrials.gov Identifier: NCT01374022
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- 2012
3. Royleanones Derivatives. Evaluation of the Antibacterial Activity
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Rijo, P, Marques, CG, Simões, MF, Araújo, ME, Duarte, A, and Repositório da Universidade de Lisboa
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Made available in DSpace on 2015-12-30T10:19:18Z (GMT). No. of bitstreams: 0 Previous issue date: 2003
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- 2003
4. Jugular foramen schwannoma: case report
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Tavares, Joana R. P, Sampaio, Andre Luiz Lopes, Ferreira, Denise B. L, Cavalheiro, Jalusa B, Araújo, Mercêdes F. S, and Oliveira, A. C. P
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Fugular foramen ,Tumors ,Schwannoma ,Medicine ,Otorhinolaryngology ,RF1-547 - Abstract
Introduction: Lesions of the jugular foramen are uncommon, and the paragangliomas are the most common tumors in this region, followed by schwannomas. Schwannomas are benign tumors, of slow growth and origin mainly in the sensitive cranial nerves. When located in the jugular foramen, the patients present with clinically auditory hearing loss, ataxia and headache, and cranial nerves IX, X and XI alterations are also common. Surgical excision is the therapeutic choice. Case Report: We reported the case of a young adult patient who sought the Otorhinolaryngology service at the School Hospital of Brasília for investigation of otalgia and chronic infection in the left ear, associated with tinnitus, hypacusis and tumor in the external ear canal. Initial diagnostic investigation by computed tomography and biopsy was inconclusive. New biopsy and magnetic resonance imaging were performed and definitive diagnosis of jugular foramen schwannoma was given. The lesion was then defined as type D by the classification of Kaye-Pellet, for it involved the jugular foramen, presented format of dumbbell and intra and extracranial components. The patient underwent a craniotomy and partial excision of the tumor. He did not have sequels of the procedure, although the otologic symptoms persisted. Final Comments: This case is an example of a rare disease. No more than two hundred cases have been reported in the literature and the clinical presentation is uncommon. The diagnostic and therapeutic challenge is faced by Otorhinolaryngology and Neurosurgery teams who are scheduling a new combined surgical procedure for a complete excision of the tumor.
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- 2009
5. Do the methods for cleaning the base of brackets used in indirect bonding interfere with adhesion to tooth enamel?
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Simão CEPCN, Silva ALFD, Araújo ME, and Caldas SGFR
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- Animals, Cattle, Aluminum Oxide chemistry, Dental Stress Analysis, Materials Testing, Resin Cements chemistry, Ethanol, Water chemistry, Random Allocation, Orthodontic Brackets, Dental Enamel drug effects, Shear Strength, Dental Bonding methods, Surface Properties
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Objective: The objective of this study was to evaluate the shear bond strength of metal brackets bonded with indirect bonding, under different surface treatment protocols., Material and Methods: 40 bovine teeth were randomly divided into four groups (n = 10), according to the type of surface treatment: G1 = 70% alcohol, G2 = air/water spray, G3 = 100-µm aluminum oxide blasting, G4 = direct boning. After drying, the standard Edgewise central incisor brackets were bonded with light-cured resin. The brackets were moved from the plaster models by means of a transfer tray made with condensation silicone, and bonded to the surface of the enamel with self-curing adhesive. The samples were submitted to shear tests by a universal test machine. Data were analyzed with SPSS 20.0 by the one-way ANOVA test and the Tukey post-test., Results: No statistically significant difference (p=0.174) was observed between the mean forces measured between the group for shear strength values of the groups during the test: G1 (5.33 MPa), G2 (3.52 MPa) and G3 (4.58 MPa)., Conclusion: The bracket surface treatment protocols presented similarities in shear bond strength test. However, alcohol 70% and oxide blasting presented higher absolute values of resistance than the water group.
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- 2024
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6. Traces of oil in sea turtle feces.
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de Souza Dias da Silva MF, Zanardi-Lamardo E, Valcarcel Rojas LA, de Oliveira Alves MD, Chimendes da Silva Neves V, and de Araújo ME
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- Animals, Environmental Monitoring, Ecosystem, Turtles, Petroleum analysis, Petroleum Pollution, Polycyclic Aromatic Hydrocarbons analysis, Water Pollutants, Chemical analysis
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In 2019, an oil spill hit the Brazilian Northeast coast causing impact to several ecosystems, including sea turtles' breeding and feeding areas. This study aimed to investigate whether sea turtles were impacted by this oil disaster, correlating the oil found inside feces with a sandy-oiled sample collected on the beach some days after the accident. The fecal samples were collected in the upper mid-littoral reef areas during three consecutive days in February 2020. The results suggested that sea turtles consumed algae contaminated by petroleum. Hydrocarbons composition of oil inside feces was similar to the sandy-oiled sample, suggesting they were the same. Lighter aliphatic and polycyclic aromatic compounds were missing, indicating both sandy-oiled and oil inside the feces had experienced significant evaporation prior to collection. Although the long-term damage is still unknown, the data are novel and relevant to support future research and alert authorities about the risks to sea turtles., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)
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- 2024
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7. Orthodontics in the oral health care network of the Unified Health System (SUS).
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Martins FC, Santos BRMD, Crosato EM, Teixeira MCL, Gabriel M, Araújo ME, Goes PSA, and Carrer FCA
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- Humans, Brazil, Dioctyl Sulfosuccinic Acid, Delivery of Health Care, Oral Health, Dental Care
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This observational study aimed to describe and analyze data from two external evaluations of the National Program for Improving Access to and Quality of Dental Specialty Centers (PMAQ CEO), held in 2014 and 2018 in Brazil, which evaluated Dental Specialty Centers (CEO) using a national and census approach. We selected questions through a search in the microdata of the first and second evaluations. The groups were analyzed independently. To compare the groups, nonparametric tests were performed (Mann Whitney U). The formulated hypotheses were: there would be no differences between the data of these groups (h0) and there would be differences between the data of these groups (h1). For qualitative nominal variables, frequency distribution was verified and association tests were performed (chi-square test). The significance level for this study was set at 5%. We observed that orthodontic treatments were found in about 13% of the CEO. Regarding human resources, most professionals were specialists or had MSc or PhD degrees; were civil servants; had been hired by direct administration; or had been hired via public tender. Regarding the work process and inclusion of the CEO in the health care network, we observed a greater number of services that use single and electronic medical records, greater presence of services monitoring and analyzing goals, greater knowledge about monthly average of absenteeism (for 2018); and larger number of services with referrals from primary health care centers (for 2014). Expanding the view on orthodontics and including preventive, interceptive, and corrective treatments at different points in health care networks are essential strategies for achieving comprehensive care in universal health systems.
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- 2024
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8. Correlation between Placido-disc and rotating Scheimpflug keratometric findings before and after corneal crosslinking in children with keratoconus.
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Polido J, Xavier Dos Santos Araújo ME, H Wakamatsu T, T Lopes B, Alexander JG, Cabral T, Ambrósio R Jr, and Freitas D
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- Brazil, Child, Cornea, Corneal Topography methods, Cross-Linking Reagents therapeutic use, Humans, Photosensitizing Agents therapeutic use, Prospective Studies, Riboflavin therapeutic use, Astigmatism drug therapy, Keratoconus diagnosis, Keratoconus drug therapy, Photochemotherapy methods
- Abstract
Purpose: To evaluate correlation between Placido-disc and rotating Scheimpflug keratometric findings in children with progressive keratoconus (KC) before and after corneal crosslinking (CXL) and investigate whether these limits of agreement varied according to disease severity., Setting: Department of Ophthalmology of São Paulo Hospital, São Paulo, Brazil., Design: Prospective nonrandomized open study., Methods: Data obtained using rotating Scheimpflug-based tomography and Placido-disc-based topography devices were collected from preoperative and last follow-up postoperative children with KC operated on using standard CXL protocol. Correlation and agreement analyses were performed between the 2 devices before and after CXL to obtain keratometric (K) findings., Results: 44 eyes from 44 patients aged 8 to 16 years were analyzed at all timepoints. All parameters were found to be strongly correlated before ( r = 0.84 to 0.99, P < .001) and after ( r = 0.93 to 0.99, P < .001) CXL. The mean Scheimpflug measurements of flat K, steep K, Kmax, mean K, and corneal astigmatism were higher than Placido-disc measurements in a preoperative period. This mean difference decreased in postoperative, but, with exception of Kmax and corneal astigmatism, Scheimpflug measurements remained higher. The mean parameter measurements from both devices decreased after CXL; 95% limits of agreement between instruments were wide for all parameters and decreased in postoperative and in mild KC., Conclusions: Keratometry measurements obtained using rotating Scheimpflug and Placido-disc technology were found to be closely correlated but not interchangeable before and after CXL in pediatric patients. Agreement between devices was better after CXL and in mild KC than in advanced KC., Competing Interests: Disclosures: R. Ambrósio is a consultant for Oculus Optikgeräte GmbH, Carl Zeiss Meditec and Mediphacos Ltda. The other authors have no conflict of interest., (Copyright © 2022 Published by Wolters Kluwer on behalf of ASCRS and ESCRS.)
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- 2022
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9. Pediatric Crosslinking: Current Protocols and Approach.
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Polido J, Dos Xavier Santos Araújo ME, Alexander JG, Cabral T, Ambrósio R Jr, and Freitas D
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Keratoconus (KC) is likely to be more aggressive in the pediatric population, with a higher risk of progression and visual loss. Several techniques have been proposed for corneal crosslinking (CXL) so far. The standard CXL (SCXL) technique, or the Dresden Protocol, originally developed by Wollensak et al., has been shown to be safe and effective in the pediatric KC group. With similar efficacy to the conventional method, the accelerated CXL (ACXL) protocols proposed a reduced UVA exposure time by increasing the intensity of UVA irradiation. Transepithelial CXL (TCXL), considered an "epithelium-on" method, emerged as a strategy to improve safety and reduce postoperative complications and discomfort. For thinner corneas, we can highlight the use of hypoosmolar riboflavin and new studies, such as contact lens-assisted CXL (CACXL), the epithelial-island CXL (EI-CXL), and the Sub400 protocol. In addition to the different protocols used, another factor that changes CXL results is the type of carrier used: dextran-based or hydroxypropyl methylcellulose-based (HPMC) riboflavin solutions. There are several ways to perform a CXL surgery, and it is still unclear which method is the safest and most effective in the pediatric group. This review of the literature in English, available in PubMed, provides an update on corneal CXL in the pediatric KC group, exploring the data on the techniques currently used and under investigation, including their advantages, efficacy, safety profiles, risks, and cost analyses., (© 2022. The Author(s).)
- Published
- 2022
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10. Retinal damage related to high-intensity light-emitting diode exposure: An in vivo study.
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Araújo ME, Paies MB, Arrais AB, Lobo FL, de Lima RRM, and Caldas SGFR
- Subjects
- Animals, Disease Models, Animal, Humans, Jordan, Male, Rats, Rats, Wistar, Light, Retina pathology
- Abstract
Introduction: The objective of this investigation was to evaluate the effects of high-intensity light-emitting diode (LED) light from a curing device on the retinas of Wistar rats., Methods: Six male Wistar rats were used, and their ocular structures were the focus of this study. During the photostimulation of each animal, the right eye of the animal, considered the control sample, was covered with a removable polyvinyl chloride cap, and the contralateral eye, the experimental sample, was exposed to high-intensity LED light, 3200 mW/cm
2 (VALO Ortho; Ultradent Products, South Jordan, Utah) for 144 seconds from a distance of 30 cm. The animals were exposed to the LED light 3 times on the same day to investigate if any acute inflammatory changes in the retina occurred. Seven days after the photostimulation sessions, the animals were anesthetized and perfused with paraformaldehyde solution. After which, the eyes were resected and processed histologically. The histologic sections were analyzed stereologically and histomorphometrically to measure the parameters of the retina under investigation., Results: There was a statistically significant increase in total retinal volume in the experimental group because of the increased volume of the ganglion cell layers, inner plexiform layers, outer nuclear layers, and the cone and rod extensions. There was no statistically significant difference in terms of density. However, there was a statistically significant increase in the nuclear area of the cells in all the studied layers in the group exposed to high-intensity LED light. In addition, hyperchromatic cells that are suggestive of pyknosis were observed., Conclusions: An acute but short protocol of exposure of high-intensity LED light to the eye caused morphometric alterations in the retinal structures, specifically in the nuclear area of the photosensitive cells., (Copyright © 2021 American Association of Orthodontists. Published by Elsevier Inc. All rights reserved.)- Published
- 2022
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11. Simulation of Organic Liquid Product Deoxygenation through Multistage Countercurrent Absorber/Stripping Using CO 2 as Solvent with Aspen-HYSYS: Process Modeling and Simulation.
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Junior MRDS, Costa EC, Ferreira CC, Bernar LP, da Silva MP, de Andrade Mâncio A, Santos MC, da Mota SAP, de Castro DAR, Junior SD, Borges LEP, Araújo ME, and Machado NT
- Subjects
- Computer Simulation, Palm Oil, Solvents, Carbon Dioxide, Hydrocarbons
- Abstract
In this work, the deoxygenation of organic liquid products (OLP) obtained through the thermal catalytic cracking of palm oil at 450 °C, 1.0 atmosphere, with 10% (wt.) Na
2 CO3 as a catalyst, in multistage countercurrent absorber columns using supercritical carbon dioxide (SC-CO2 ) as a solvent, with an Aspen-HYSYS process simulator, was systematically investigated. In a previous study, the thermodynamic data basis and EOS modeling necessary to simulate the deoxygenation of OLP was presented. This work addresses a new flowsheet, consisting of 03 absorber columns, 10 expansions valves, 10 flash drums, 08 heat exchanges, 01 pressure pump, and 02 make-ups of CO2 , aiming to improve the deacidification of OLP. The simulation was performed at 333 K, 140 bar, and (S/F) = 17; 350 K, 140 bar, and (S/F) = 38; 333 K, 140 bar, and (S/F) = 25. The simulation shows that 81.49% of OLP could be recovered and that the concentrations of hydrocarbons in the extracts of absorber-01 and absorber-02 were 96.95 and 92.78% (wt.) on a solvent-free basis, while the bottom stream of absorber-03 was enriched in oxygenated compounds with concentrations of up to 32.66% (wt.) on a solvent-free basis, showing that the organic liquid products (OLP) were deacidified and SC-CO2 was able to deacidify the OLP and obtain fractions with lower olefin contents. The best deacidifying condition was obtained at 333 K, 140 bar, and (S/F) = 17.- Published
- 2022
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12. Evaluation of Marrubium vulgare Growing Wild in Tunisia for Its Potential as a Dietary Supplement.
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Rezgui M, Basma M, Neng N, Nogueira JM, Bettaieb Ben-Kaab L, and Machado Araújo ME
- Abstract
Marrubium vulgare L., known as horehound, is a widespread and widely known plant that is used in beer breweries and also as a traditional remedy in Tunisia. In this study, methanolic extracts of plants harvested from five different locations were investigated for their antioxidant activities using three assays (ferric reducing power, radical scavenging activity, and β -carotene-linoleic acid bleaching assay) as well as the total phenolic content. The mineral composition of the plant was also investigated concerning the following elements: Fe, Mg, Ca, Cu, Zn, Mn, K, and three heavy metals, Ni, Pb, and Cd. Marrubiin, the major bioactive diterpenoid lactone, was quantified by NMR in the samples. The essential oils were obtained by hydrodistillation, and their radical scavenging activity was investigated. The toxicity of essential oils was evaluated against Artemia salina (the brine shrimp larva). The essential oil showed a weak radical scavenging activity and low toxicity. Data obtained from the five different locations showed that the antioxidant activity, as well as the total phenolic and marrubiin content, were strongly affected by the harvest sites. The metal content in the samples showed differences with the harvest location, but there was always a great abundance of calcium, magnesium, and potassium.
- Published
- 2021
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13. Simulation of Organic Liquid Products Deoxygenation by Multistage Countercurrent Absorber/Stripping Using CO 2 as Solvent with Aspen-HYSYS: Thermodynamic Data Basis and EOS Modeling.
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Costa EC, de Araújo Silva W, Menezes EGO, da Silva MP, Cunha VMB, de Andrade Mâncio A, Santos MC, da Mota SAP, Araújo ME, and Machado NT
- Abstract
In this work, the thermodynamic data basis and equation of state (EOS) modeling necessary to simulate the fractionation of organic liquid products (OLP), a liquid reaction product obtained by thermal catalytic cracking of palm oil at 450 °C, 1.0 atmosphere, with 10% (wt.) Na
2 CO3 as catalyst, in multistage countercurrent absorber/stripping columns using supercritical carbon dioxide (SC-CO2 ) as solvent, with Aspen-HYSYS was systematically investigated. The chemical composition of OLP was used to predict the density (ρ), boiling temperature (Tb ), critical temperature (Tc ), critical pressure (Pc ), critical volume (Vc ), and acentric factor (ω) of all the compounds present in OLP by applying the group contribution methods of Marrero-Gani, Han-Peng, Marrero-Pardillo, Constantinou-Gani, Joback and Reid, and Vetere. The RK-Aspen EOS used as thermodynamic fluid package, applied to correlate the experimental phase equilibrium data of binary systems OLP-i /CO2 available in the literature. The group contribution methods selected based on the lowest relative average deviation by computing Tb , Tc , Pc , Vc , and ω. For n -alkanes, the method of Marrero-Gani selected for the prediction of Tc , Pc and Vc , and that of Han-Peng for ω. For alkenes, the method of Marrero-Gani selected for the prediction of Tb and Tc , Marrero-Pardillo for Pc and Vc , and Han-Peng for ω. For unsubstituted cyclic hydrocarbons, the method of Constantinou-Gani selected for the prediction of Tb , Marrero-Gani for Tc , Joback for Pc and Vc , and the undirected method of Vetere for ω. For substituted cyclic hydrocarbons, the method of Constantinou-Gani selected for the prediction of Tb and Pc , Marrero-Gani for Tc and Vc , and the undirected method of Vetere for ω. For aromatic hydrocarbon, the method of Joback selected for the prediction of Tb , Constantinou-Gani for Tc and Vc , Marrero-Gani for Pc , and the undirected method of Vetere for ω. The regressions show that RK-Aspen EOS was able to describe the experimental phase equilibrium data for all the binary pairs undecane-CO2 , tetradecane-CO2 , pentadecane-CO2 , hexadecane-CO2 , octadecane-CO2 , palmitic acid-CO2 , and oleic acid-CO2 , showing average absolute deviation for the liquid phase (AADx) between 0.8% and 1.25% and average absolute deviation for the gaseous phase (AADy) between 0.01% to 0.66%.- Published
- 2021
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14. Sedentary behavior, unhealthy food consumption and dental caries in 12-year-old schoolchildren: a population-based study.
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Bomfim RA, Frias AC, Cascaes AM, Mazzilli LEN, Souza LB, Carrer FCA, and Araújo ME
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- Brazil epidemiology, Child, DMF Index, Fluoridation, Humans, Prevalence, Sedentary Behavior, Dental Caries epidemiology, Dental Caries etiology
- Abstract
This study analyzed the association between sedentary behavior (SB), unhealthy food consumption, and dental caries amongst 12-year-old schoolchildren. An epidemiological survey was carried out in the five largest cities (> 80,000 inhabitants) of the State of Mato Grosso do Sul, Brazil. Data were collected on decayed, missing and filled teeth index (DMFT), sociodemographic characteristics, SB, unhealthy food consumption, and water fluoridation status. The analysis was based on the theoretical framework established by J Sisson. Structural equation models were performed to test the association of dental caries experience with sociodemographic, contextual, and behavioral factors. The mean DMFT index in the five cities was 1.02 (95%CI: 0.39-1.66). Higher sedentary behavior (more than 2 hours/day) [standardized coefficient (SC) = 0.21 95%CI: 0.07-0.39] and higher unhealthy food consumption (more than 4 times/week) [SC = 0.23 (0.10-0.45)] were associated with higher DMFT index than their counterparts. Also, cities with fluoridated water were associated with lower DMFT index [SC = -0.85 (-1.20--0.50)]. Families who had a per capita income above the poverty line had a direct association with unhealthy food consumption [SC = -0.24 (-0.38--0.11)]. Unhealthy food consumption mediated the association of sedentary behavior on DMFT index [SC=0.07 (0.02-0.13)]. Sensitivity analysis confirmed the findings. Sedentary behavior mediated by unhealthy food consumption had a significant association with dental caries experience. Public policies must address transdisciplinary actions to reduce sedentary behavior and unhealthy food consumption and promote water fluoridation.
- Published
- 2021
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15. Household food insecurity, dental caries and oral-health-related quality of life in Brazilian Indigenous adults.
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Soares GH, Mota JMS, Mialhe FL, Biazevic MGH, Araújo ME, and Michel-Crosato E
- Subjects
- Adult, Brazil epidemiology, Cross-Sectional Studies, Food Insecurity, Food Supply, Humans, Socioeconomic Factors, Dental Caries epidemiology, Quality of Life
- Abstract
Food insecurity is a complex phenomenon that affects the health and wellbeing of vulnerable families. The aim of this study was to investigate the relationship between household food insecurity, dental caries, oral health-related quality of life, and social determinants of health among Indigenous adults. A cross-sectional study was conducted among Kaingang adults aged 35-44 years old from the Guarita Indigenous Land, Brazil. Food insecurity was assessed through the EBIA scale. Dental caries was assessed using the DMFT index. Participants answered the OHIP-14 questionnaire and a structured interview. Descriptive and multivariate analyzes using Poisson regression models were performed. The final sample included 107 adults from 97 households. Approximately 95% lived in food insecure families. Severe food insecurity was present in 58% of the households. The phenomenon was associated to the Bolsa Família benefit, household size, and greater perception of oral health impacts on quality of life. The high number of families affected by food insecurity reveals the social vulnerability of the Kaingang people. Food insecurity in Kaingangs adults is associated to oral health perception and social determinants of health.
- Published
- 2021
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16. Diversity patterns of reef fish along the Brazilian tropical coast.
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Araújo ME, Mattos FMG, Melo FPL, Chaves LCT, Feitosa CV, Lippi DL, Félix Hackradt FC, Hackradt CW, Nunes JLS, Leão ZMAN, Kikuchi RKP, Ferreira Junior AV, Pereira PHC, Macedo CHR, Sampaio CLS, and Feitosa JLL
- Subjects
- Animals, Brazil, Biodiversity, Coral Reefs, Fishes
- Abstract
Causal mechanisms for broad-scale reef fish diversity patterns are poorly understood and current knowledge is limited to trends of species richness. This work compared the effects of ecological drivers on components of fish diversity across reefs spanning over 2.000 km of the tropical Brazilian coastline. A quarter of communities' diversity is accountable to common and dominant species, while remaining species are rare. Low-latitude sites were more diverse in rare species. Communities along the coast share common and dominant species, which display high densities across all reefs, but differ in rare species that show abundance peaks in particular reef morphotypes. The disproportionate distribution of rare species reveals a higher vulnerability of these communities to impacts and stochastic density fluctuations. Uneven conservation efforts directed to these morphotypes pose a threat to the maintenance of a paramount component of the reef fish diversity represented by rare species., (Copyright © 2020 Elsevier Ltd. All rights reserved.)
- Published
- 2020
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17. Primary Pediatric Keratoplasty: Etiology, Graft Survival, and Visual Outcome.
- Author
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Xavier Dos Santos Araújo ME, Santos NC, Souza LB, Sato EH, and de Freitas D
- Subjects
- Case-Control Studies, Child, Child, Preschool, Corneal Opacity congenital, Corneal Opacity physiopathology, Female, Humans, Infant, Kaplan-Meier Estimate, Male, Retrospective Studies, Visual Acuity physiology, Corneal Opacity surgery, Graft Survival physiology, Keratoplasty, Penetrating
- Abstract
Purpose: Interventional study to evaluate the etiology, visual outcome and survival of corneal transplantation in children and to identify the risk factors associated with graft failure., Design: Retrospective, interventional consecutive case series., Methods: Medical records of every child 7 years of age or younger who underwent primary penetrating keratoplasty at Department of Ophthalmology, Federal University of São Paulo were reviewed. The parameters evaluated were indications for keratoplasty, graft survival, and postoperative visual acuity (VA) improvement. Children underwent ophthalmologic examination before and after corneal graft, including VA, assessed by the preferential looking test and visual evoked potential. The analysis of transplant survival was performed using the Kaplan-Meier method., Results: Fifty-six penetrating transplants were performed in 51 eyes of 43 children. The patients were divided into 2 groups: congenital (72.5%) and acquired (17.5%) corneal opacity. The main indication was congenital glaucoma (29.4%). The overall Kaplan-Meier graft survival rates were 64.7% in the postoperative average follow-up period of 24 months. There was no significant difference in graft survival between the congenital and acquired groups (Mantel-Cox P = .1031). There was significant improvement in VA in both groups (P = .0022 for congenital and P < .0001 for acquired). Rejection and diagnosis of congenital glaucoma were risk factors for graft failure., Conclusions: Congenital glaucoma was the main indication for corneal transplantation and despite the difficulties, prolonged survival and improved VA can be achieved in pediatric transplant. Complications such as rejection and early glaucoma were significantly associated with graft failure., (Copyright © 2020 Elsevier Inc. All rights reserved.)
- Published
- 2020
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18. Artisanal fishers, consumers and the environment: immediate consequences of the oil spill in Pernambuco, Northeast Brazil.
- Author
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Araújo ME, Ramalho CWN, and Melo PW
- Subjects
- Animals, Brazil, Commerce, Disasters, Environment, Humans, Ecosystem, Fisheries, Petroleum poisoning, Petroleum Pollution, Water Pollutants, Chemical poisoning
- Published
- 2020
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19. In vitro digestion, antioxidant and antiacetylcholinesterase activities of two species of Ruta: Ruta chalepensis and Ruta montana.
- Author
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Khadhri A, Bouali I, Belkhir S, Mokded R, Smiti S, Falé P, Araújo ME, and Serralheiro ML
- Subjects
- Antioxidants isolation & purification, Biphenyl Compounds chemistry, Cholinesterase Inhibitors isolation & purification, Chromatography, High Pressure Liquid, Drug Stability, Gastric Juice chemistry, Gastrointestinal Agents isolation & purification, Iron chemistry, Oxidation-Reduction, Pancreatic Juice chemistry, Phytotherapy, Picrates chemistry, Plant Extracts isolation & purification, Plant Leaves, Plants, Medicinal, Ruta classification, Antioxidants pharmacology, Cholinesterase Inhibitors pharmacology, Digestion, Gastrointestinal Agents pharmacology, Oxidative Stress drug effects, Plant Extracts pharmacology, Ruta chemistry
- Abstract
Context: Ruta genus (Rutaceae) is abundantly used and described in the most ancient systematic records of medical practice of the Mediterranean world. In Tunisia, this genus is represented by two medicinal and aromatic shrubs: Ruta chalepensis L. and Ruta montana L., Objective: This study investigates the antioxidant and acetylcholinesterase inhibition (AChE) activities before and after in vitro gastrointestinal metabolism of leaf decoction of R. chalepensis and R. montana., Materials and Methods: We study, in vitro, the effect of the gastrointestinal juices gastric (1.75 mL) or pancreatic (2.5 mL) juices, on the biological activity by the measurement of the antioxidant activity and AChE inhibition during 4 h of decoction extract obtained from the leaves of the two species of Ruta., Results: The results showed that the ability to inhibit the AChE enzyme was similar; being the greatest inhibitory activity exhibited by the ethanol extract (IC
50 =- Published
- 2017
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20. Stability of beta-titanium T-loop springs preactivated by gradual curvature.
- Author
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Caldas SGFR, Martins RP, Araújo ME, Galvão MR, Silva Júnior RSD, and Martins LP
- Subjects
- Biomechanical Phenomena, Materials Testing, Random Allocation, Statistics, Nonparametric, Stress, Mechanical, Time Factors, Dental Alloys chemistry, Dental Stress Analysis, Orthodontic Appliance Design, Orthodontic Space Closure instrumentation, Orthodontic Wires, Titanium chemistry
- Abstract
Objective: Evaluate changes in the force system of T-Loop Springs (TLS) preactivated by curvature, due to stress relaxation., Methods: Ninety TLSs measuring 6 x 10 mm, produced out with 0.017 x 0.025-in TMA® wire and preactived by gradual curvature, were randomly distributed into nine groups according to time point of evaluation. Group 1 was tested immediately after spring preactivation and stress relief, by trial activation. The other eight groups were tested after 24, 48 and 72 hours, 1, 2, 4, 8 and 12 weeks, respectively. Using a moment transducer coupled to a digital extensometer indicator adapted to a universal testing machine, the amount of horizontal force, moment and moment-to-force ratios were recorded at every 0.5 mm of deactivation from 5 mm of the initial activation, in an interbracket distance of 23 mm., Results: The horizontal forces decreased gradually among the groups (p< 0.001) and the moments showed a significant and slow decrease over time among the groups (p< 0.001). All groups produced similar M/F ratios (p= 0.532), with no influence of time., Conclusions: The TLSs preactivated by curvature suffered a gradual deformation over time, which affected the force system, specifically the moments, which affected the horizontal forces produced.
- Published
- 2017
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21. Biocatalytic Synthesis of Flavonoid Esters by Lipases and Their Biological Benefits.
- Author
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de Araújo ME, Franco YE, Messias MC, Longato GB, Pamphile JA, and Carvalho PO
- Subjects
- Acylation, Anti-Infective Agents, Anti-Inflammatory Agents, Antioxidants metabolism, Biocatalysis, Cosmetics, Fatty Acids metabolism, Solubility, Esters metabolism, Flavonoids metabolism, Lipase metabolism, Plants chemistry
- Abstract
Several studies have described important biological activities of flavonoids such as coronary heart disease prevention, hepatoprotective, anti-inflammatory and anticancer activities, enzyme inhibition activity, and antibacterial, antifungal, and antiviral activities. Flavonoids show promising activity as natural plant-based antioxidants due to their antioxidant and free radical scavenging properties. However, their primary applications as antioxidants in the pharmaceutical, cosmetic, and food industries are limited because of their moderately hydrophilic nature. Enzymatic acylation of natural polyphenols with fatty acids or other acyl donors has been suggested for improving the lipophilic nature of the glycosylated flavonoids. This approach increases flavonoid solubility and stability in lipophilic systems. Acylation of flavonoids with different acyl donors may also introduce beneficial properties to the molecule, such as penetration through the cell membrane and improved antioxidant, antimicrobial, anti-inflammatory, antiproliferative, cytogenetic, and enzyme inhibition activities. Chemical methods for the synthesis of flavonoid esters lead to the formation of side products and the simultaneous decomposition of the flavonoids due to harsh reaction conditions. In contrast, biocatalytic acylation of flavonoids by lipases offers advantages associated to the wide availability of these enzymes, their low cost, chemo-, regio-, and enantioselectivity, mild condition processing and non-requirement of cofactors. This article is focused on the recent development of lipase-catalyzed synthesis of flavonoid esters and the impact of the acylation reaction on their biological activities., (Georg Thieme Verlag KG Stuttgart · New York.)
- Published
- 2017
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22. Synthesis of structured triacylglycerols enriched in n-3 fatty acids by immobilized microbial lipase.
- Author
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Araújo ME, Campos PR, Alberto TG, Contesini FJ, and Carvalho PO
- Subjects
- Chromatography, Gas, Enzyme Stability, Spectrometry, Mass, Electrospray Ionization, Carboxylic Ester Hydrolases chemistry, Enzymes, Immobilized, Fatty Acids, Omega-3 chemical synthesis, Triglycerides chemistry
- Abstract
The search for new biocatalysts has aroused great interest due to the variety of micro-organisms and their role as enzyme producers. Native lipases from Aspergillus niger and Rhizopus javanicus were used to enrich the n-3 long-chain polyunsaturated fatty acids content in the triacylglycerols of soybean oil by acidolysis with free fatty acids from sardine oil in solvent-free media. For the immobilization process, the best lipase/support ratios were 1:3 (w/w) for Aspergillus niger lipase and 1:5 (w/w) for Rhizopus javanicus lipase using Amberlite MB-1. Both lipases maintained constant activity for 6 months at 4°C. Reaction time, sardine-free fatty acids:soybean oil mole ratio and initial water content of the lipase were investigated to determine their effects on n-3 long-chain polyunsaturated fatty acids incorporation into soybean oil. Structured triacylglycerols with 11.7 and 7.2% of eicosapentaenoic acid+docosahexaenoic acid were obtained using Aspergillus niger lipase and Rhizopus javanicus lipase, decreasing the n-6/n-3 fatty acids ratio of soybean oil (11:1 to 3.5:1 and 4.7:1, respectively). The best reaction conditions were: initial water content of lipase of 0.86% (w/w), sardine-free faty acids:soybean oil mole ratio of 3:1 and reaction time of 36h, at 40°C. The significant factors for the acidolysis reaction were the sardine-free fatty acids:soybean oil mole ratio and reaction time. The characterization of structured triacylglycerols was obtained using easy ambient sonic-spray ionization mass spectrometry. The enzymatic reaction led to the formation of many structured triacylglycerols containing eicosapentaenoic acid, docosahexaenoic acid or both polyunsaturated fatty acids., (Copyright © 2016 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda. All rights reserved.)
- Published
- 2016
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23. Review of anticancer mechanisms of isoquercitin.
- Author
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Orfali Gd, Duarte AC, Bonadio V, Martinez NP, de Araújo ME, Priviero FB, Carvalho PO, and Priolli DG
- Abstract
This review was based on a literature search of PubMed and Scielo databases using the keywords "quercetin, rutin, isoquercitrin, isoquercitin (IQ), quercetin-3-glucoside, bioavailability, flavonols and favonoids, and cancer" and combinations of all the words. We collected relevant scientific publications from 1990 to 2015 about the absorption, bioavailability, chemoprevention activity, and treatment effects as well as the underlying anticancer mechanisms of isoquercitin. Flavonoids are a group of polyphenolic compounds widely distributed throughout the plant kingdom. The subclass of flavonols receives special attention owing to their health benefits. The main components of this class are quercetin, rutin, and IQ, which is a flavonoid and although mostly found as a glycoside, is an aglycone (lacks a glycoside side chain). This compound presents similar therapeutic profiles to quercetin but with superior bioavailability, resulting in increased efficacy compared to the aglycone form. IQ has therapeutic applications owing to its wide range of pharmacological effects including antioxidant, antiproliferative, anti-inflammatory, anti-hypertensive, and anti-diabetic. The protective effects of IQ in cancer may be due to actions on lipid peroxidation. In addition, the antitumor effect of IQ and its underlying mechanism are related to interactions with Wnt signaling pathway, mixed-lineage protein kinase 3, mitogen-activated protein kinase, apoptotic pathways, as well proinflammatory protein signaling. This review contributed to clarifying the mechanisms of absorption, metabolism, and actions of IQ and isoquercitrin in cancer.
- Published
- 2016
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24. Leprosy in Brazilian counties bordering Paraguay: Mato Grosso do Sul State, 2001-2011.
- Author
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Ajalla ME, Oliveira De Andrade SM, Tamaki EM, Waissmann W, Deittrich SH, and Aragão Do Nascimento V
- Subjects
- Adolescent, Brazil epidemiology, Female, Humans, Male, Paraguay epidemiology, Prevalence, Time Factors, Leprosy diagnosis, Leprosy epidemiology
- Abstract
Background: In Mato Grosso do Sul state, Brazil, the dry border shared with Paraguay is a territory marked by facilities in the flow of goods, services and people, bringing difficulties for surveillance of communicable diseases., Purpose: The purpose of this study is to characterise leprosy epidemiologically in dry border municipalities of Mato Grosso do Sul in Brazil with contiguous urban areas with neighbouring Paraguayan counties, in the period 2001-2011., Methods: This is an exploratory descriptive investigation that includes the four dry border municipalities of Mato Grosso do Sul (Coronel Sapucaia, Paranhos, Ponta Porã, and Sete Quedas) in Brazil whose urban areas are contiguous with Paraguay. Data comprised the period 2001-2011., Results: The rates of leprosy detection and prevalence oscillated along the study period, increasing in the last 2 years investigated. The detection rate was 3.3/10,000 in 2011, up from 1.7/10,000 in 2009. Prevalence was 5.3/10,000 in 2011, up from 2.5/10,000 in 2009. The Virchowian disease form was predominant in 8 of the 11 years investigated. Most patients were male, with limited formal education (44.2% with less than 4 years of study)., Conclusion: In the border of Brazil, most (greater than 70%) of the cases detected were classified as multibacillary. The higher coefficient found in Brazilian municipalities was the Virchowian clinical form, which can influence the operational classification in multibacillary. The predominance of the Virchowian clinical form, larger number of patients in rural areas and children under 15 years of age provides new information on the manifestations of the disease in the border territories. The study revealed that municipalities with contiguous cross-border urban areas with Paraguay have unique epidemiological features that need to be addressed by policies focusing leprosy as a public health priority.
- Published
- 2016
25. Characterization of the antioxidant activity of aglycone and glycosylated derivatives of hesperetin: an in vitro and in vivo study.
- Author
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de Souza VT, de Franco ÉP, de Araújo ME, Messias MC, Priviero FB, Frankland Sawaya AC, and de Oliveira Carvalho P
- Subjects
- Animals, Antioxidants chemistry, Antioxidants pharmacology, Gene Expression Regulation, Enzymologic drug effects, Hesperidin chemistry, Hesperidin pharmacology, In Vitro Techniques, Lipids blood, Liver drug effects, Liver enzymology, Male, Rats, Rats, Wistar, Superoxide Dismutase metabolism, Antioxidants administration & dosage, Hesperidin administration & dosage, Xanthine Oxidase antagonists & inhibitors
- Abstract
The flavonoids are mainly present in Citrus fruits as their glycosyl derivatives. This study was conducted comparing in vitro xanthine oxidase inhibitory activity of the aglycone hesperetin and its glycosylated forms (hesperidin and G-hesperidin) and their effects on the plasma lipid profile and the oxidative-antioxidative system (TBARS and antioxidant enzymes) in rats. The concentrations of the major conjugated metabolites in rat plasma after oral administration of these compounds were also determined. Wistar male rats were randomly assigned to three groups (n=6) supplemented for 30 days with 1 mmol/kg body mass of hesperetin, hesperidin or G-hesperidin. Hesperetin was a stronger xanthine oxidase inhibitor (IC50=53 μM and Ki=17.3 μM) than the glycosylate derivatives. Supplementation with the three compounds led to a lower (more favorable) atherogenic index, and an antioxidant preventive effect from the increase of hepatic superoxide dismutase was observed associated to HT supplementation, possibly because of the higher level of hesperetin-glucuronide in rat plasma., (Copyright © 2015 John Wiley & Sons, Ltd.)
- Published
- 2016
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26. Isolation of Macrophage Early and Late Endosomes by Latex Bead Internalization and Density Gradient Centrifugation.
- Author
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Lamberti G, de Araújo ME, and Huber LA
- Subjects
- Animals, Humans, Cell Fractionation methods, Centrifugation, Density Gradient methods, Endosomes chemistry, Macrophages chemistry, Microspheres
- Abstract
Immortalized macrophage lines and primary macrophages display the ability to internalize small latex beads through the endocytic pathway. This protocol describes a simple and robust method for separating endocytic organelles from macrophages on a sucrose gradient, taking advantage of the significantly lower density of the organelles containing latex beads compared with other intracellular organelles. The latex beads are retained in the endosomes as they mature; therefore, harvesting cells at different time points after internalization permits the purification of different organelle fractions, particularly early and late endosomes., (© 2015 Cold Spring Harbor Laboratory Press.)
- Published
- 2015
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27. Purification of Early and Late Endosomes.
- Author
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de Araújo ME, Lamberti G, and Huber LA
- Subjects
- Animals, Humans, Cell Fractionation methods, Endosomes chemistry, Proteome analysis, Proteome isolation & purification
- Abstract
Proteomic analysis of early and late endosomes has been constrained by the limited purity of the endosomal fractions that can be achieved by biochemical methods. Here we briefly review endocytic pathways, and then introduce fractionation strategies that have been used to improve the purity of isolated endosomes. In addition, we describe innovative proteomics analysis methods that have been shown to partially circumvent the limitations found in the enrichment steps., (© 2015 Cold Spring Harbor Laboratory Press.)
- Published
- 2015
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28. Homogenization of Mammalian Cells.
- Author
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de Araújo ME, Lamberti G, and Huber LA
- Subjects
- Animals, Cell Fractionation instrumentation, Cells, Cultured, Humans, Mammals, Reproducibility of Results, Syringes, Cell Fractionation methods
- Abstract
Homogenization is the name given to the methodological steps necessary for releasing organelles and other cellular constituents as a free suspension of intact individual components. Most homogenization procedures used for mammalian cells (e.g., cavitation pump and Dounce homogenizer) rely on mechanical force to break the plasma membrane and may be supplemented with osmotic or temperature alterations to facilitate membrane disruption. In this protocol, we describe a syringe-based homogenization method that does not require specialized equipment, is easy to handle, and gives reproducible results. The method may be adapted for cells that require hypotonic shock before homogenization. We routinely use it as part of our workflow to isolate endocytic organelles from mammalian cells., (© 2015 Cold Spring Harbor Laboratory Press.)
- Published
- 2015
- Full Text
- View/download PDF
29. Isolation of Early and Late Endosomes by Density Gradient Centrifugation.
- Author
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de Araújo ME, Lamberti G, and Huber LA
- Subjects
- Animals, Cells, Cultured, Humans, Mammals, Cell Fractionation methods, Centrifugation, Density Gradient methods, Endosomes
- Abstract
Density gradient centrifugation is a common method for separating intracellular organelles. During centrifugation, organelles float or sediment until they reach their isopycnic position within the gradient. The density of an organelle depends on its content, size, shape, and the lipid:protein ratio. The degree of separation between different organelles will therefore be highly dependent on how different their isopycnic points are in a given buffer. Separation will also depend on the medium used to prepare the gradient, whether it is sucrose (the most common) or an alternative. Here we describe the use of both continuous and discontinuous (step) gradients to isolate endocytic organelles., (© 2015 Cold Spring Harbor Laboratory Press.)
- Published
- 2015
- Full Text
- View/download PDF
30. New In Vitro Studies on the Bioprofile of Genista tenera Antihyperglycemic Extract.
- Author
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Batista D, Falé PL, Serralheiro ML, Araújo ME, Madeira PJ, Borges C, Torgal I, Goulart M, Justino J, Martins A, and Rauter AP
- Abstract
The inhibition of α-glucosidase and glucose-6-phosphatase, two enzymes involved in the carbohydrate metabolism, is an important target to control glycaemia on individuals with type 2 diabetes. In this work we report for the first time the inhibition of both enzymes by the antihyperglycemic n-butanol extract from Genista tenera (Fabaceae). This extract decreased α-glucosidase and glucose-6-phosphatase activities to 0.97 and 80.25 %, respectively, being more effective than acarbose, and phlorizin, the positive controls, which reduced enzymes activities only to 17.39 and 96.06 %. Once inflammation and oxidative stress are related to diabetic impairments, the anti-inflammatory activity of the extract was also evaluated, through its inhibitory activity over COX-1 enzyme (47.5 % inhibition). Moreover, after induction of oxidative stress by UV radiation, the viability of irradiated rat liver hepatoma cells exposed to the extract was significantly higher (67.82 %) than that promoted by ascorbic acid, the positive control (45.05 %). In addition, the stability of the extract under gastrointestinal conditions was evaluated by HPLC-DAD-ESI-MS/MS. Flavonoid diglycosides were identified as the main constituents of the extract, and no alterations in the chemical composition nor in the antioxidant activity were observed after in vitro digestion with artificial gastric and pancreatic juices.
- Published
- 2015
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- View/download PDF
31. Correlations Between Allergen-Specific IgE Serum Levels in Patients With Ocular Allergy.
- Author
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Polido JG, Cabral T, Perini Pde R, Fernandes Mde F, de Freitas D, dos Santos Araújo ME, and Serracarbassa PD
- Subjects
- Acaridae immunology, Adolescent, Adult, Animals, Antigens, Dermatophagoides immunology, Blood Cell Count, Child, Child, Preschool, Cockroaches immunology, Eosinophils immunology, Female, Follow-Up Studies, Humans, Insect Proteins immunology, Leukocyte Count, Male, Middle Aged, Allergens immunology, Conjunctivitis, Allergic immunology, Immunoglobulin E blood
- Abstract
Purpose: To evaluate ocular allergies in patients at the Hospital do Servidor Publico Estadual de Sao Paulo (HSPE) and the correlations with serum allergen-specific immunoglobulin E levels., Methods: We performed a longitudinal study of patients with ocular allergies who were treated at the Cornea and Immunology and Allergy Department. Patients underwent an ophthalmologic examination to identify their primary presenting signs and symptoms. The allergy types were divided into 4 groups. We conducted the following laboratory tests and measurements: blood count, eosinophil count, total serum IgE, and specific IgE., Results: Among 61 patients, 16 (26.2%) had a clinical diagnosis of seasonal allergic conjunctivitis, 23 (37.7%) had perennial allergic conjunctivitis, 19 (31.1%) had vernal keratoconjunctivitis, and 3 (4.9%) had atopic keratoconjunctivitis. Mixed dust mites were positive in 94.9% of patients. Dermatophagoides pteronyssinus (dp) and Dermatophagoides farinae (df) antigens were positive in 93.2% of patients followed by Blattella germanica, Blomia tropicalis, and mixed animal epithelia (81%, 75.9%, and 25.8%, respectively)., Conclusions: Perennial allergic conjunctivitis was the most prevalent disorder and demonstrated higher positivity in class V/VI for specific antigens (mixed dust mites, dp, and df), indicating high antigenicity. Dust mites, D. pteronyssinus, D. farinae, B. germanica, and B. tropicalis were the primary triggers of the studied ocular allergies.
- Published
- 2015
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- View/download PDF
32. The influence of fire-coral colony size and agonistic behaviour of territorial damselfish on associated coral reef fish communities.
- Author
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Leal IC, de Araújo ME, da Cunha SR, and Pereira PH
- Subjects
- Animals, Biodiversity, Population Density, Agonistic Behavior physiology, Anthozoa physiology, Behavior, Animal, Coral Reefs, Fishes physiology
- Abstract
Branching hydrocorals from the genus Millepora play an important ecological role in South Atlantic reefs, where branching scleractinian corals are absent. Previous studies have shown a high proportion of reef fish species using branching fire-coral colonies as shelter, breeding, and feeding sites. However, the effects of Millepora spp. colony size and how the agonistic behaviour of a competitive damselfish affect the associated reef fish community are still unknown. The present study examined how fire-coral colony volume and the presence of a highly territorial and aggressive damselfish (Brazilian endemic Stegastes fuscus) affects the reef fish community associated with the fire-coral Millepora alcicornis. M. alcicornis colonies were surveyed from September 2012 to April 2013 at Tamandaré Reefs off Northeast Brazil. Our results show that the abundance and richness of coral associated fish was positively correlated with M. alcicornis coral colony volume. Additionally, behaviour of S. fuscus, the most abundant reef fish species found associated with fire-coral colonies (almost 57% of the fish community), was also influenced by fire-coral colony volume. There was a clear trend of increased agonistic behaviour and feeding on coral polyps as colony volume increased. This trend was reversed for the non-occupational swimming category, which decreased as M. alcicornis colony volume increased. Behavioural ontogenetic changes were also detected for S. fuscus individuals. Juveniles mainly showed two distinct behaviours: sheltered on coral branches and feeding on coral polyps. In contrast, adults presented greater equitability among the behavioural categories, mostly non-occupational swimming around coral colonies and agonistic behaviour. Lastly, S. fuscus individuals actively defended fire-coral colonies from intruders. A large number of agonistic interactions occurred against potential food competitors, which were mainly roving herbivores, omnivores, and sessile invertebrate feeders. To our knowledge, the present study provides the first evidence that through habitat competition, the presence of S. fuscus may affect reef fish communities associated with M. alcicornis coral colonies. Our findings also indicate that S. fuscus uses M. alcicornis coral colonies as part of their territory for shelter and foraging. In conclusion, M. alcicornis fire-coral colonies are extremely important habitats for reef fishes and the size and presence of a territorial damselfish are relevant variables for associated reef fish community., (Copyright © 2015 Elsevier Ltd. All rights reserved.)
- Published
- 2015
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33. SLC38A9 is a component of the lysosomal amino acid sensing machinery that controls mTORC1.
- Author
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Rebsamen M, Pochini L, Stasyk T, de Araújo ME, Galluccio M, Kandasamy RK, Snijder B, Fauster A, Rudashevskaya EL, Bruckner M, Scorzoni S, Filipek PA, Huber KV, Bigenzahn JW, Heinz LX, Kraft C, Bennett KL, Indiveri C, Huber LA, and Superti-Furga G
- Subjects
- Animals, Cell Line, Humans, Mechanistic Target of Rapamycin Complex 1, Mice, Monomeric GTP-Binding Proteins metabolism, Nucleotides metabolism, Amino Acid Transport Systems metabolism, Amino Acids metabolism, Lysosomes metabolism, Multiprotein Complexes metabolism, TOR Serine-Threonine Kinases metabolism
- Abstract
Cell growth and proliferation are tightly linked to nutrient availability. The mechanistic target of rapamycin complex 1 (mTORC1) integrates the presence of growth factors, energy levels, glucose and amino acids to modulate metabolic status and cellular responses. mTORC1 is activated at the surface of lysosomes by the RAG GTPases and the Ragulator complex through a not fully understood mechanism monitoring amino acid availability in the lysosomal lumen and involving the vacuolar H(+)-ATPase. Here we describe the uncharacterized human member 9 of the solute carrier family 38 (SLC38A9) as a lysosomal membrane-resident protein competent in amino acid transport. Extensive functional proteomic analysis established SLC38A9 as an integral part of the Ragulator-RAG GTPases machinery. Gain of SLC38A9 function rendered cells resistant to amino acid withdrawal, whereas loss of SLC38A9 expression impaired amino-acid-induced mTORC1 activation. Thus SLC38A9 is a physical and functional component of the amino acid sensing machinery that controls the activation of mTOR.
- Published
- 2015
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- View/download PDF
34. Nuss procedure for pectus excavatum repair: critical appraisal of the evidence.
- Author
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Araújo ME, Penha Ada P, Westphal FL, Silva MT, and Galvão TF
- Subjects
- Humans, Orthopedic Procedures methods, Treatment Outcome, Funnel Chest surgery
- Abstract
Objective: To evaluate the effectiveness and safety of correction of pectus excavatum by the Nuss technique based on the available scientific evidence., Methods: We conducted an evidence synthesis following systematic processes of search, selection, extraction and critical appraisal. Outcomes were classified by importance and had their quality assessed by the Grading of Recommendations Assessment, Development and Evaluation (GRADE)., Results: The process of selection of items led to the inclusion of only one systematic review, which synthesized the results of nine observational studies comparing the Nuss and Ravitch procedures. The evidence found was rated as poor and very poor quality. The Nuss procedure has increased the incidence of hemothorax (RR = 5.15; 95% CI: 1.07; 24.89), pneumothorax (RR = 5.26; 95% CI: 1.55; 17.92) and the need for reintervention (RR = 4.88; 95% CI: 2.41; 9.88) when compared to the Ravitch. There was no statistical difference between the two procedures in outcomes: general complications, blood transfusion, hospital stay and time to ambulation. The Nuss operation was faster than the Ravitch (mean difference [MD] = -69.94 minutes, 95% CI: -139.04, -0.83)., Conclusion: In the absence of well-designed prospective studies to clarify the evidence, especially in terms of aesthetics and quality of life, surgical indication should be individualized and the choice of the technique based on patient preference and experience of the team.
- Published
- 2014
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35. Multidrug resistant Mycobacterium tuberculosis: a retrospective katG and rpoB mutation profile analysis in isolates from a reference center in Brazil.
- Author
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de Freitas FA, Bernardo V, Gomgnimbou MK, Sola C, Siqueira HR, Pereira MA, Fandinho FC, Gomes HM, Araújo ME, Suffys PN, Marques EA, and Albano RM
- Subjects
- Adult, Bacterial Typing Techniques, Brazil epidemiology, Cluster Analysis, DNA-Directed RNA Polymerases, Demography, Female, Humans, Incidence, Male, Mycobacterium tuberculosis classification, Phylogeny, Polymorphism, Restriction Fragment Length, Retrospective Studies, Tuberculosis, Multidrug-Resistant epidemiology, Bacterial Proteins genetics, Catalase genetics, Drug Resistance, Multiple, Bacterial genetics, Mutation genetics, Mycobacterium tuberculosis genetics, Mycobacterium tuberculosis isolation & purification, Tuberculosis, Multidrug-Resistant genetics
- Abstract
Background: Multidrug resistance is a critical factor in tuberculosis control. To gain better understanding of multidrug resistant tuberculosis in Brazil, a retrospective study was performed to compare genotypic diversity and drug resistance associated mutations in Mycobacterium tuberculosis isolates from a national reference center., Methods and Findings: Ninety-nine multidrug resistant isolates from 12 Brazilian states were studied. Drug-resistance patterns were determined and the rpoB and katG genes were screened for mutations. Genotypic diversity was investigated by IS6110-RFLP and Luminex 47 spoligotyping. Mutations in rpoB and katG were seen in 91% and 93% of the isolates, respectively. Codon 315 katG mutations occurred in 82.8% of the isolates with a predominance of the Ser315Thr substitution. Twenty-five isolates were clustered in 11 groups with identical IS6110-RFLP patterns while 74 showed unique patterns with no association between mutation frequencies or susceptibility profiles. The most prevalent spoligotyping lineages were LAM (47%), T (17%) and Haarlen (12%). The Haarlen lineage showed a higher frequency of codon 516 rpoB mutations while codon 531 mutations prevailed in the other isolates., Conclusions: Our data suggest that there were no major multidrug resistant M. tuberculosis strains transmitted among patients referred to the reference center, indicating an independent acquisition of resistance. In addition, drug resistance associated mutation profiles were well established among the main spoligotyping lineages found in these Brazilian multidrug resistant isolates, providing useful data for patient management and treatment.
- Published
- 2014
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36. Toxicity of ionic liquids prepared from biomaterials.
- Author
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Gouveia W, Jorge TF, Martins S, Meireles M, Carolino M, Cruz C, Almeida TV, and Araújo ME
- Subjects
- Amino Acids chemistry, Animals, Anions chemistry, Anions toxicity, Artemia drug effects, Bacillus subtilis drug effects, Biocompatible Materials chemistry, Bromides chemistry, Cations chemistry, Cations toxicity, Escherichia coli drug effects, HeLa Cells, Humans, Ionic Liquids chemistry, Amino Acids toxicity, Biocompatible Materials toxicity, Bromides toxicity, Ionic Liquids toxicity
- Abstract
In search of environmentally-friendly ionic liquids (ILs), 14 were prepared based on the imidazolium, pyridinium and choline cations, with bromide and several amino acids as anions. Good yields were obtained in the synthesis of pyridinium ILs and those prepared from choline and amino acids. Four of the ILs synthesized from choline and the amino acids arginine, glutamine, glutamic acid and cystine are described here for the first time. The toxicity of the synthesized ILs was checked against organisms of various levels of organization: the crustacean Artemia salina; Human cell HeLa (cervical carcinoma); and bacteria with different types of cell wall, Bacillus subtilis and Escherichia coli. The toxicity was observed to depend on both the cation and anion. Choline-amino acid ILs showed a remarkable low toxicity to A. salina and HeLa cell culture, ten times less than imidazolium and pyridinium ILs. None of ionic liquids exhibited marked toxicity to bacteria, and the effect was 2-3 orders of magnitude smaller than that of the antibiotic chloramphenicol., (Copyright © 2013 Elsevier Ltd. All rights reserved.)
- Published
- 2014
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37. Enzymatic de-glycosylation of rutin improves its antioxidant and antiproliferative activities.
- Author
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de Araújo ME, Moreira Franco YE, Alberto TG, Sobreiro MA, Conrado MA, Priolli DG, Frankland Sawaya AC, Ruiz AL, de Carvalho JE, and de Oliveira Carvalho P
- Subjects
- Cell Line, Tumor, Glycosylation, Humans, Hydrolysis, Molecular Structure, Antioxidants chemistry, Antioxidants pharmacology, Cell Proliferation drug effects, Fungal Proteins chemistry, Glycoside Hydrolases chemistry, Multienzyme Complexes chemistry, Penicillium enzymology, Rutin chemistry, Rutin pharmacology, beta-Glucosidase chemistry
- Abstract
Bioavailability and biological properties of flavonoid glycosides can be improved after the enzymatic hydrolysis of specific glycosyl groups. In this study, we evaluate the antioxidant and antiproliferative potential of rutin after enzymatic hydrolysis performed by α-l-rhamnosidases (hesperidinase from Penicillium sp. and naringinase from Penicillium decumbens) previously heated at 70°C for 30 min to inactivate the undesirable β-d-glucosidase activity. The highest in vitro antioxidant activity determined by DPPH radical scavenging was achieved with rutin hydrolyzed by hesperidinase. Rutin was predominantly bioconverted into quercetin-3-glucoside. There was no statistical difference between xanthine oxidase inhibition by rutin before and after hydrolysis. However, in vitro inhibitory activity against ten human tumor cell lines showed that hydrolyzed rutin exerted a more potent antiproliferative effect than quercetin and rutin on various cancer cell lines, specially glioma, and ovarian and breast adenocarcinomas. These results indicate that quercetin-3-glucoside could be a promising functional derivative obtained by rutin hydrolysis., (Copyright © 2013 Elsevier Ltd. All rights reserved.)
- Published
- 2013
- Full Text
- View/download PDF
38. Stability of the endosomal scaffold protein LAMTOR3 depends on heterodimer assembly and proteasomal degradation.
- Author
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de Araújo ME, Stasyk T, Taub N, Ebner HL, Fürst B, Filipek P, Weys SR, Hess MW, Lindner H, Kremser L, and Huber LA
- Subjects
- Adaptor Proteins, Signal Transducing genetics, Animals, Blotting, Western, Cells, Cultured, Embryo, Mammalian cytology, Endosomes metabolism, Fibroblasts cytology, Fibroblasts metabolism, HEK293 Cells, HeLa Cells, Humans, Mice, Mice, Knockout, Microscopy, Confocal, Protein Stability, Proteins genetics, Proteins metabolism, Proteolysis, Reverse Transcriptase Polymerase Chain Reaction, Ubiquitin metabolism, Adaptor Proteins, Signal Transducing chemistry, Adaptor Proteins, Signal Transducing metabolism, Proteasome Endopeptidase Complex metabolism, Protein Multimerization
- Abstract
LAMTOR3 (MP1) and LAMTOR2 (p14) form a heterodimer as part of the larger Ragulator complex that is required for MAPK and mTOR1 signaling from late endosomes/lysosomes. Here, we show that loss of LAMTOR2 (p14) results in an unstable cytosolic monomeric pool of LAMTOR3 (MP1). Monomeric cytoplasmic LAMTOR3 is rapidly degraded in a proteasome-dependent but lysosome-independent manner. Mutational analyses indicated that the turnover of the protein is dependent on ubiquitination of several lysine residues. Similarly, other Ragulator subunits, LAMTOR1 (p18), LAMTOR4 (c7orf59), and LAMTOR5 (HBXIP), are degraded as well upon the loss of LAMTOR2. Thus the assembly of the Ragulator complex is monitored by cellular quality control systems, most likely to prevent aberrant signaling at the convergence of mTOR and MAPK caused by a defective Ragulator complex.
- Published
- 2013
- Full Text
- View/download PDF
39. [Congenital corneal anesthesia: case reports].
- Author
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Lana FP, Oliveira RC, Vieira AC, and Araújo ME
- Subjects
- Adolescent, Child, Child, Preschool, Corneal Diseases therapy, Humans, Infant, Treatment Outcome, Corneal Diseases congenital, Pain Insensitivity, Congenital therapy
- Abstract
Case series of nine patients with congenital corneal anesthesia, six of them showed systemic changes in association with the ocular status. Three patients were submitted to electromyography, two showed isolated bilateral ophthalmic ramus alteration. Two patients had initial visual acuity better than 20/60 and six had final best corrected visual acuity better than 20/60 at the last visit. All of them were treated surgically and developed cornea opacities of variable sizes. Treatment of corneal congenital anesthesia must be performed as soon as possible to avoid corneal opacification. Systemic investigation, close follow-up and preparing the family for longterm and multidisciplinary approach are crucial to maintain the ocular health.
- Published
- 2012
- Full Text
- View/download PDF
40. The effectiveness of the Pilates method: reducing the degree of non-structural scoliosis, and improving flexibility and pain in female college students.
- Author
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Alves de Araújo ME, Bezerra da Silva E, Bragade Mello D, Cader SA, Shiguemi Inoue Salgado A, and Dantas EH
- Subjects
- Adolescent, Adult, Back Pain physiopathology, Female, Humans, Lumbar Vertebrae physiology, Muscle Stretching Exercises methods, Range of Motion, Articular physiology, Scoliosis physiopathology, Students, Young Adult, Back Pain therapy, Exercise Movement Techniques methods, Physical Therapy Modalities, Scoliosis therapy
- Abstract
Objective: To evaluate the effectiveness of Pilates with regard to the degree of scoliosis, flexibility and pain., Method: The study included 31 female students divided into two groups: a control group (CG = 11), which had no therapeutic intervention, and an experimental group (EG = 20), which underwent Pilates-based therapy. We used radiological goniometry measurements to assess the degree of scoliosis, standard goniometry measurements to determine the degree of flexibility and the scale of perceived pain using the Borg CR 10 to quantify the level of pain., Results: The independent t test of the Cobb angle (t = - 2.317, p = 0.028), range of motion of trunk flexion (t = 3.088, p = 0.004) and pain (t = -2.478, p = 0.019) showed significant differences between the groups, with best values in the Pilates group. The dependent t test detected a significant decrease in the Cobb angle (Δ% = 38%, t = 6.115, p = 0.0001), a significant increase in trunk flexion (Δ% = 80%, t = -7.977, p = 0.0001) and a significant reduction in pain (Δ% = 60%, t = 7.102, p = 0.0001) in the EG. No significant difference in Cobb angle (t = 0.430, p = 0.676), trunk flexion, (t = 0.938p = 0.371) or pain (t = 0.896, p = 0.391) was found for the CG., Conclusion: The Pilates group was better than control group. The Pilates method showed a reduction in the degree of non-structural scoliosis, increased flexibility and decreased pain., (Copyright © 2011 Elsevier Ltd. All rights reserved.)
- Published
- 2012
- Full Text
- View/download PDF
41. [Strategies and results of the oral cancer prevention campaign among the elderly in São Paulo, Brazil, 2001 to 2009].
- Author
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Martins JS, Abreu SC, Araújo ME, Bourget MM, Campos FL, Grigoletto MV, and Almeida FC
- Subjects
- Adult, Age Distribution, Aged, Aged, 80 and over, Brazil epidemiology, Early Detection of Cancer, Health Promotion methods, Humans, Male, Middle Aged, Mouth Neoplasms diagnosis, Mouth Neoplasms epidemiology, Oral Health, Health Promotion organization & administration, Mouth Neoplasms prevention & control
- Abstract
Objective: To describe the strategies and results obtained by the early diagnosis and prevention of an oral cancer campaign targeting the population aged 60 years or older developed since 2001 in the state of São Paulo., Methods: The main strategies used to develop the campaign were described based on the review of documents issued by the Health Ministry, National Cancer Institute, São Paulo State Health Department, Oncocentro Foundation of São Paulo, São Paulo City Health Department, School of Public Health at the University of São Paulo (USP), and Santa Marcelina Health Care Center. The impact of the campaign on the incidence of new cases of oral cancer in the target population was evaluated., Results: In 2001, 90,886 elderly were examined vs. 629,613 in 2009. The following strategies were identified: training of professionals, development of printed materials to guide municipal governments in developing the campaign and using standardized codes and criteria, guidelines for data consolidation, establishment of patient referral flows, practical training with a specialist at the basic health care unit after the follow-up examination of individuals presenting changes in soft tissues, and increase in the number of oral diagnosis services. Between 2005 and 2009, there was a significant reduction in the rate of confirmed cases of oral cancer per 100,000 individuals examined, from 20.89 to 11.12 (P = 0.00003)., Conclusions: The campaign was beneficial to the oral health of the elderly and could be extended to include other age groups and regions of the country. It may also provide a basis for the development of oral cancer prevention actions in other countries, as long as local characteristics are taken into account.
- Published
- 2012
- Full Text
- View/download PDF
42. Facile synthesis of oxo-/thioxopyrimidines and tetrazoles C-C linked to sugars as novel non-toxic antioxidant acetylcholinesterase inhibitors.
- Author
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Figueiredo JA, Ismael MI, Pinheiro JM, Silva AM, Justino J, Silva FV, Goulart M, Mira D, Araújo ME, Campoy R, and Rauter AP
- Subjects
- Acetylcholinesterase metabolism, Animals, Antioxidants chemistry, Antioxidants pharmacology, Antioxidants toxicity, Carbohydrates chemistry, Carbohydrates toxicity, Cattle, Cell Line, Cell Survival drug effects, Cholinesterase Inhibitors chemistry, Cholinesterase Inhibitors pharmacology, Cholinesterase Inhibitors toxicity, Humans, Linoleic Acid chemistry, beta Carotene chemistry, Antioxidants chemical synthesis, Carbohydrates chemical synthesis, Carbohydrates pharmacology, Chemistry Techniques, Synthetic methods, Cholinesterase Inhibitors chemical synthesis, Pyrimidines chemistry, Tetrazoles chemistry
- Abstract
Microwave-assisted synthesis of oxo-/thioxopyrimidines and tetrazoles linked to furanoses with D-xylo and D-ribo configuration, and to a D-galacto pyranose is reported and compared to conventional methods. Reaction of dialdofuranoses and dialdopyranoses with a β-keto ester and urea or thiourea under microwave irradiation at 300 W gave in 10 min the target molecules containing the 2-oxo- or 2-thioxo-pyrimidine ring in high yield. The tetrazole-derived compounds were obtained in two steps by reaction of the formyl group with hydroxylamine hydrochloride, copper sulfate, triethylamine and dicyclohexylcarbodiimide to give an intermediate nitrile, which was then treated with sodium azide. The use of microwave irradiation in the latter step also resulted in a considerably shorter reaction time (10 min) compared to hours under conventional heating to obtain a complete starting materials conversion. Acetylcholinesterase inhibition ranged from 20% to 80% for compounds concentration of 100 μg/mL, demonstrating the potential of this family of compounds for the control of Alzheimer's disease symptoms. Most of the compounds showed antioxidant activity in the β-carotene/linoleic acid assay, some of them exhibiting IC(50) values in the same order of magnitude as those of gallic acid. The bioactive compounds did not show cytotoxic effects to human lymphocytes using the MTT method adapted for non-adherent cells, nor genotoxicity determined by the short-term in vitro chromosomal aberration assay., (Copyright © 2011 Elsevier Ltd. All rights reserved.)
- Published
- 2012
- Full Text
- View/download PDF
43. Reorganization of secondary and tertiary health care levels: impact on the outcomes of oral cancer screening in the São Paulo State, Brazil.
- Author
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Almeida FC, Cazal C, Pucca Júnior GA, Silva DP, Frias AC, and Araújo ME
- Subjects
- Aged, Aged, 80 and over, Brazil epidemiology, Female, Health Promotion, Humans, Longitudinal Studies, Male, Middle Aged, Mouth Neoplasms epidemiology, Retrospective Studies, Delivery of Health Care trends, Early Detection of Cancer, Mouth Neoplasms diagnosis
- Abstract
This study presents the strategies for prevention and early detection of oral cancer by means of screening in the elderly population of São Paulo, the richest and the most populous state of Brazil. This research was a retrospective longitudinal study based on the analysis of secondary data. The variables - number of participating cities, coverage of screening, and number of suspicious and confirmed cases of oral cancer - were divided into two periods: 2001-2004 and 2005-2008. Data were analyzed statistically by the chi-square test at 5% significance level. The implementation of a nationwide public oral health policy in 2004 and the reorganization of the secondary and tertiary health care were evaluated as mediator factors able to interfere in the achieved outcomes. From 2001 to 2008, 2,229,273 oral examinations were performed. There was an addition of 205 participating cities by the end of the studied period (p<0.0001). The coverage of oral cancer screening increased from 4.1% to 16% (p<0.0001). There was a decrease in the number of suspicious lesions (from 9% in 2005 to 5% in 2008) (p<0.0001) and in the rate of confirmed oral cancer cases per 100,000 examinations (from 20.89 in 2001 to 10.40 in 2008) (p<0.0001). After 8 years of screening, there was a decrease in the number of suspicious lesions and confirmed cases of oral cancer in the population. The reorganization of secondary and tertiary health care levels of oral care seems to have contributed to modify these numbers, having a positive impact on the outcomes of oral cancer screening in the São Paulo State.
- Published
- 2012
- Full Text
- View/download PDF
44. Optimized enzymatic synthesis of hesperidin fatty acid esters in a two-phase system containing ionic liquid.
- Author
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de Araújo ME, Contesini FJ, Franco YE, Sawaya AC, Alberto TG, Dalfré N, and Carvalho Pde O
- Subjects
- Analysis of Variance, Candida enzymology, Esters, Fungal Proteins, Hesperidin chemical synthesis, Imidazoles chemistry, Kinetics, Biocatalysis, Decanoic Acids chemical synthesis, Enzymes, Immobilized chemistry, Hesperidin analogs & derivatives, Ionic Liquids chemistry, Lipase chemistry
- Abstract
Response surface methodology (RSM) based on a five-level, three-variable central composite design (CCD) was employed for modeling and optimizing the conversion yield of the enzymatic acylation of hesperidin with decanoic acid using immobilized Candida antarctica lipase B (CALB) in a two-phase system containing [bmim]BF(4). The three variables studied (molar ratio of hesperidin to decanoic acid, [bmim]BF(4)/acetone ratio and lipase concentration) significantly affected the conversion yield of acylated hesperidin derivative. Verification experiments confirmed the validity of the predicted model. The lipase showed higher conversion degree in a two-phase system using [bmim]BF(4) and acetone compared to that in pure acetone. Under the optimal reaction conditions carried out in a single-step biocatalytic process when the water content was kept lower than 200 ppm, the maximum acylation yield was 53.6%.
- Published
- 2011
- Full Text
- View/download PDF
45. Latex beads internalization and quantitative proteomics join forces to decipher the endosomal proteome.
- Author
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Guimarães de Araújo ME, Huber LA, and Stasyk T
- Abstract
The proteome analysis of endocytic compartments has been constrained by the limited purity of the organelle fractions obtained by current biochemical methods. Duclos and coworkers have developed a novel method to isolate highly purified endosomal organelles based on small latex beads internalization followed by gradient centrifugation and successfully combined it with a redundant peptide counting method to compare the relative abundance of proteins in organelles. The presence of bona fide markers in their respective subcellular organelles and the identification of several new endosomal-associated proteins, attested the applicability of their combinatory approach. Future applications of this strategy may deliver a comprehensive endosomal proteome chart: from the identification of the key players to the determination of time and signaling-dependent proteome changes. As a long-term perspective, such an approach may unveil new clues to the molecular mechanisms underlining human diseases associated with endosomal biogenesis defects.
- Published
- 2011
- Full Text
- View/download PDF
46. [Application of corrective formula for intraocular pressure changes in patients that underwent LASIK].
- Author
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Silva TG, Polido JG, Pinheiro MV, Silva AL, Goldbach L, Mascaro VL, Serracarbassa PD, and Araújo ME
- Subjects
- Adult, Algorithms, Astigmatism surgery, Cornea pathology, Corneal Topography, Female, Humans, Male, Middle Aged, Myopia surgery, Organ Size, Postoperative Period, Prospective Studies, Tonometry, Ocular, Treatment Outcome, Young Adult, Intraocular Pressure physiology, Keratomileusis, Laser In Situ
- Abstract
Purpose: To compare the intraocular pressure (IOP) pre and post LASIK, correlating it to changes in central corneal thickness (CCT) and average simulated keratometry (K), as well as verifying the results of a corrective formula previously proposed., Methods: Longitudinal prospective study conducted in outpatients that underwent to LASIK. Patients underwent complete ophthalmic examination, previously and 2 months after the surgery. Intraocular pressure was evaluated with Goldmann applanation tonometer between 9 am and 11 am, average simulated keratometry was evaluated using corneal topography and central corneal thickness was measured with ultrasound pachymetry, been considered the average of three measurements. Two patients were excluded due to surgery or eye disease, and previous use of topical steroids over the past three months. The surgeries were performed according to standard procedures. The formula [real IOP = IOP measured + (540 - ECC)/71 + (43 - K)/2.7 + 0.75 mmHg] proposed for correcting intraocular pressure was used., Results: Fifteen eyes of eight patients were evaluated, age ranged from 24 to 46 years (mean: 31.37 ± 7.27). There was a statistically significant difference between the measurements of intraocular pressure, central corneal thickness and average simulated keratometry pre and post-LASIK. (p=0.0001). It was observed that each 1D corrected underestimated the IOP 1.06 ± 0.59 mmHg (0.11 a 1.89 mmHg). The use of the corrective formula lead to 80% of eyes within 2.50 mmHg of preoperative intraocular pressure. Although, the two sets of data are statistically different (p=0.0266)., Conclusions: Post LASIK eyes presented lower intraocular pressure than preoperatively. Intraocular pressure was moderately correlated to central corneal thickness and weakly correlated to average simulated keratometry. With the use of the corrective formula, we were able to determine that 80% were within 2.50 mmHg of the preoperative intraocular pressure.
- Published
- 2011
- Full Text
- View/download PDF
47. [Popularization of oral self-examination: an example of non formal education--Part II].
- Author
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de Almeida FC, da Silva DP, Amoroso MA, Dias Rde B, Crivello O Jr, and de Araújo ME
- Subjects
- Brazil, Humans, Health Education methods, Mouth, Oral Health, Self-Examination
- Abstract
One of the largest challenges of the universities, especially the public ones, is to transpose the scientific knowledge produced into their walls for the general population. The non-formal education is an important tool, and yet very little used by researchers and professors to approximate the scientific knowledge. The oral cancer reaches more than 11.000 Brazilians a year. In spite of the high incidence, this pathology is still little known by the general population and part of the medical and dental class. Basing on the epidemic data in researches and scientific papers, the oral cancer was the elect theme for the action in education and communication of the first national campaign with no government character of prevention in oral cancer, being a great example of as that can be made. This paper intends to describe the methodology used in communication and the results obtained in this successful experience.
- Published
- 2011
- Full Text
- View/download PDF
48. Bioactivity studies and chemical profile of the antidiabetic plant Genista tenera.
- Author
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Rauter AP, Martins A, Lopes R, Ferreira J, Serralheiro LM, Araújo ME, Borges C, Justino J, Silva FV, Goulart M, Thomas-Oates J, Rodrigues JA, Edwards E, Noronha JP, Pinto R, and Mota-Filipe H
- Subjects
- Animals, Antioxidants pharmacology, Blood Glucose, Cholinesterase Inhibitors pharmacology, Flavonoids pharmacology, Hypoglycemic Agents adverse effects, Hypoglycemic Agents pharmacology, Plant Extracts adverse effects, Plant Extracts chemistry, Plant Extracts pharmacology, Rats, Diabetes Mellitus, Experimental drug therapy, Flavonoids therapeutic use, Genista, Hypoglycemic Agents therapeutic use, Phytotherapy, Plant Extracts therapeutic use
- Abstract
Aim of the Study: Genista tenera is a plant endemic to the island of Madeira and is used in folk medicine to control diabetes. In the present work we evaluate the antihyperglycaemic activity of its n-butanol extract and determine its chromatographic profile. In addition, this extract, the ethyl acetate and diethyl ether plant extracts were studied in order to assess the plant antioxidant and acetylcholinesterase inhibitory activities, as well as its cyto- and genotoxicities., Materials and Methods: HPLC-DAD-ESI-MS was used to analyze the flavonoid profile of the n-butanol extract. The antihyperglycaemic activity of this extract was performed over streptozotocin induced diabetic Wistar rats (200 mg/kg, bw/day), for 15 days. Antioxidant activity (DPPH assay) and acetylcholinesterase inhibitory effect (Ellman method) were also performed. Acute cytotoxicity and genotoxicity were assessed by proliferative index quantification and the short-term chromosomal aberration technique, after exposure of lymphocytes to the extracts., Results and Conclusions: The n-butanol extract, where 21 monoglycosyl and 12 diglycosyl flavonoids were detected, significantly lowered blood glucose levels, bringing them to normal values after 15 days of treatment. The best radical scavenging activity was observed for the ethyl acetate extract (48.7% at 139.1 microg/mL), which was also the most effective one at the minimal concentration tested. The highest acetylcholinesterase inhibitory activity (77.0% at 70.0 microg/mL) was also obtained with the ethyl acetate extract. In vitro toxicity studies showed no evidence for acute cytotoxicity or genotoxicity. This is the first report on antidiabetic activity of genus Genista.
- Published
- 2009
- Full Text
- View/download PDF
49. Sexuality of women attended in family health program: a social poetic production.
- Author
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Gomes ME, Silveira LC, Petit SH, Brasileiro GM, and Almeida AN
- Subjects
- Adult, Brazil, Female, Humans, Family Health, Health Knowledge, Attitudes, Practice, Health Promotion, Sexual Behavior
- Abstract
The concept of promotion of health is one of the main axes of current health policies in Brazil. The adoption of this concept implies a change in the care model involving valuing a greater autonomy of subjects to work improving their conditions of life. From this perspective, several elements of health practices have to be reviewed, sexuality among them. Thus, we aimed at producing sexuality concepts as of the knowledge of women cared for in a health unity. This is a qualitative study adopting a social poetic approach. The results pointed out several affections that involve the concept of sexuality going beyond the biological aspect. We understand that this study may help professionals caring for women in a health unity because it broadens the concept of sexuality allowing for reflection on the nursing practice in collective health.
- Published
- 2008
- Full Text
- View/download PDF
50. Subcellular fractionation.
- Author
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de Araújo ME and Huber LA
- Subjects
- Animals, Centrifugation, Humans, Proteins isolation & purification, Reproducibility of Results, Subcellular Fractions chemistry, Subcellular Fractions metabolism, Cell Fractionation methods, Organelles chemistry, Proteins analysis
- Abstract
The successful combination of highly sensitive mass spectrometry and pre-fractionation techniques has provided a powerful tool to detect dynamic changes in low abundant regulatory proteins at the organelle level. Subcellular fractionation, being flexible, adjustable (both in cell and tissues), and allowing the analysis of proteins in their physiologic/intracellular context, has become the most commonly used preparative/enrichment method. This chapter introduces state-of-the-art subcellular fractionation protocols and briefly discuss their suitability, advantages, and limitations.
- Published
- 2007
- Full Text
- View/download PDF
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