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171 results on '"Application des ultrasons à la thérapie ( LabTAU )"'

1. Transesophageal 2D ultrasound to 3D computed tomography registration for the guidance of a cardiac arrhythmia therapy

Author: Zulma Sandoval, Cyril Lafon, Javad Alirezaie, Miguel Castro, Jean-Louis Dillenseger, Francis Bessière, Laboratoire Traitement du Signal et de l'Image (LTSI), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Santé et de la Recherche Médicale (INSERM), Ryerson University [Toronto], Application des ultrasons à la thérapie (LabTAU), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre Léon Bérard [Lyon]-Institut National de la Santé et de la Recherche Médicale (INSERM), ANR-11-TECS-0004, French National Research Agency under the Health Technology Program (ANR TecSan), ANR-11-TECS-0004,CardioUSgHIFU,Traitement des arythmies cardiaques par ultrasons focalisés guidés par échographie.(2011), ANR-17-CE19-0017,CHORUS,Développement d'une sonde HIFU trans oesophagienne pour le traitement des arythmies cardiaques(2017), Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire Traitement du Signal et de l'Image ( LTSI ), Université de Rennes 1 ( UR1 ), Université de Rennes ( UNIV-RENNES ) -Université de Rennes ( UNIV-RENNES ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ), Ryerson University (Toronto), Application des ultrasons à la thérapie ( LabTAU ), Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale ( INSERM ), ANR-11-TECS-0004,CardioUSgHIFU,Traitement des arythmies cardiaques par ultrasons focalisés guidés par échographie. ( 2011 ), ANR-17-CE19-0017,CHORUS,Design of a transesophageal HIFU probe for the treatment of cardiac arrhythmias, Dillenseger, Jean-Louis, Technologie pour la santé et l’autonomie - Traitement des arythmies cardiaques par ultrasons focalisés guidés par échographie. - - CardioUSgHIFU2011 - ANR-11-TECS-0004 - TecSan - VALID, and Développement d'une sonde HIFU trans oesophagienne pour le traitement des arythmies cardiaques - - CHORUS2017 - ANR-17-CE19-0017 - AAPG2017 - VALID
Subjects: medicine.medical_specialty, [SDV.MHEP.CHI] Life Sciences [q-bio]/Human health and pathology/Surgery, medicine.medical_treatment, [INFO.INFO-IM] Computer Science [cs]/Medical Imaging, Computed tomography, 2d ultrasound, [ SPI.SIGNAL ] Engineering Sciences [physics]/Signal and Image processing, [SDV.MHEP.CHI]Life Sciences [q-bio]/Human health and pathology/Surgery, 01 natural sciences, Imaging phantom, 030218 nuclear medicine & medical imaging, 03 medical and health sciences, 0302 clinical medicine, Esophagus, Imaging, Three-Dimensional, [SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system, 0103 physical sciences, medicine, Image Processing, Computer-Assisted, [INFO.INFO-IM]Computer Science [cs]/Medical Imaging, Humans, Radiology, Nuclear Medicine and imaging, [ SDV.IB ] Life Sciences [q-bio]/Bioengineering, [ SDV.MHEP.CHI ] Life Sciences [q-bio]/Human health and pathology/Surgery, 010301 acoustics, [SPI.SIGNAL] Engineering Sciences [physics]/Signal and Image processing, [SDV.IB] Life Sciences [q-bio]/Bioengineering, Radiological and Ultrasound Technology, medicine.diagnostic_test, business.industry, Phantoms, Imaging, Cardiac arrhythmia, Atrial fibrillation, Arrhythmias, Cardiac, Heart, [ SDV.MHEP.CSC ] Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system, medicine.disease, High-intensity focused ultrasound, [SDV.MHEP.CSC] Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system, 3. Good health, High-Intensity Focused Ultrasound Ablation, [SDV.IB]Life Sciences [q-bio]/Bioengineering, Radiology, business, Tomography, X-Ray Computed, [SPI.SIGNAL]Engineering Sciences [physics]/Signal and Image processing
Abstract: International audience; The work aims to develop a new image-processing method to improve the guidance of transesophageal high intensity focused ultrasound (HIFU) atrial fibrillation therapy. Our proposal is a novel registration approach that aligns intraoperative 2D ultrasound with preoperative 3D-CT information. This approach takes advantage of the anatomical constraints imposed at the transesophageal HIFU probe to simplify the registration process. Our proposed method has been evaluated on a physical phantom and on real clinical data.
Published: 2018
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2. Fast Volumetric Ultrasound B-Mode and Doppler Imaging with a New High-Channels Density Platform for Advanced 4D Cardiac Imaging/Therapy

Author: William Apoutou N'Djin, Jean-Yves Chapelon, Francois Varray, Lorena Petrusca, Rémi Souchon, Herve Liebgott, Magalie Viallon, Adeline Bernard, RMN et optique : De la mesure au biomarqueur, Centre de Recherche en Acquisition et Traitement de l'Image pour la Santé (CREATIS), Université Jean Monnet [Saint-Étienne] (UJM)-Hospices Civils de Lyon (HCL)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Jean Monnet [Saint-Étienne] (UJM)-Hospices Civils de Lyon (HCL)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Imagerie Ultrasonore, Application des ultrasons à la thérapie (LabTAU), Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM), Plateforme d'Imagerie Multimodale LyonTech (PILoT), ANR-11-LABX-0063,PRIMES,Physique, Radiobiologie, Imagerie Médicale et Simulation(2011), 5 - RMN et optique : De la mesure aux biomarqueurs, Centre de Recherche en Acquisition et Traitement de l'Image pour la Santé ( CREATIS ), Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon ( INSA Lyon ), Université de Lyon-Institut National des Sciences Appliquées ( INSA ) -Institut National des Sciences Appliquées ( INSA ) -Hospices Civils de Lyon ( HCL ) -Université Jean Monnet [Saint-Étienne] ( UJM ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ) -Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Institut National des Sciences Appliquées ( INSA ) -Institut National des Sciences Appliquées ( INSA ) -Hospices Civils de Lyon ( HCL ) -Université Jean Monnet [Saint-Étienne] ( UJM ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), 3 - Imagerie Ultrasonore, Application des ultrasons à la thérapie ( LabTAU ), Université de Lyon-Université de Lyon-Centre Léon Bérard [Lyon]-Institut National de la Santé et de la Recherche Médicale ( INSERM ), Plateforme d'Imagerie Multimodale LyonTech ( PILoT ), 1 - Imagerie et modélisation Vasculaires, Thoraciques et Cérébrales ( MOTIVATE ), ANR-11-IDEX-0007-02/11-LABX-0063,PRIMES,Physique, Radiobiologie, Imagerie Médicale et Simulation ( 2011 ), Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Centre Léon Bérard [Lyon]-Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: Computer science, Image quality, cardiac, [SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/Imaging, ultrasound, 4-D, fast volumetric imaging, platform, advanced imaging, power doppler, 01 natural sciences, Doppler imaging, lcsh:Technology, Imaging phantom, 030218 nuclear medicine & medical imaging, lcsh:Chemistry, 03 medical and health sciences, symbols.namesake, 0302 clinical medicine, Contrast-to-noise ratio, 0103 physical sciences, General Materials Science, 010301 acoustics, Instrumentation, lcsh:QH301-705.5, Cardiac imaging, [ SDV.IB.IMA ] Life Sciences [q-bio]/Bioengineering/Imaging, Fluid Flow and Transfer Processes, [SPI.ACOU]Engineering Sciences [physics]/Acoustics [physics.class-ph], business.industry, lcsh:T, [ SPI.ACOU ] Engineering Sciences [physics]/Acoustics [physics.class-ph], Process Chemistry and Technology, General Engineering, lcsh:QC1-999, Computer Science Applications, Transmission (telecommunications), lcsh:Biology (General), lcsh:QD1-999, lcsh:TA1-2040, symbols, Contrast ratio, business, lcsh:Engineering (General). Civil engineering (General), Doppler effect, Computer hardware, lcsh:Physics
Abstract: International audience; A novel ultrasound (US) high-channels platform is a prerequisite to open new frontiers in diagnostic and/or therapy by experimental implementation of innovative advanced US techniques. To date, a few systems with more than 1000 transducers permit full and simultaneous control in both transmission and receiving of all single elements of arrays. A powerful US platform for implementing 4-D (real-time 3-D) advanced US strategies, offering full research access, is presented in this paper. It includes a 1024-elements array prototype designed for 4-D cardiac dual-mode US imaging/therapy and 4 synchronized Vantage systems. The physical addressing of each element was properly chosen for allowing various array downsampled combinations while minimizing the number of driving systems. Numerical simulations of US imaging were performed, and corresponding experimental data were acquired to compare full and downsampled array strategies, testing 4-D imaging sequences and reconstruction processes. The results indicate the degree of degradation of image quality when using full array or downsampled combinations, and the contrast ratio and the contrast to noise ratio vary from 7.71 dB to 2.02 dB and from 2.99 dB to −7.31 dB, respectively. Moreover, the feasibility of the 4-D US platform implementation was tested on a blood vessel mimicking phantom for preliminary Doppler applications. The acquired data with fast volumetric imaging with up to 2000 fps allowed assessing the validity of common 3-D power Doppler, opening in this way a large field of applications.
Published: 2018
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3. Interferential therapy for chronic constipation in adults: The CON‐COUR randomizedcontrolled trial

Author: Veronique Vitton, François Mion, Anne‐Marie Leroi, Charlène Brochard, Benoit Coffin, Frank Zerbib, Henri Damon, Chloé Melchior, Henri Duboc, Michel Queralto, Karine Baumstarck, Assistance Publique - Hôpitaux de Marseille (APHM), Hospices Civils de Lyon (HCL), Application des ultrasons à la thérapie (LabTAU), Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM), Nutrition, Inflammation et axe Microbiote-Intestin-Cerveau (ADEN), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Institute for Research and Innovation in Biomedicine (IRIB), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institute for Research and Innovation in Biomedicine (IRIB), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Normandie Université (NU), Service de physiologie digestive, urinaire, respiratoire et de l'exercice [CHU Rouen], Hôpital Charles Nicolle [Rouen], CHU Rouen, Normandie Université (NU)-Normandie Université (NU)-CHU Rouen, Normandie Université (NU)-Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Centre d'Investigation Clinique [CHU Rouen] (CIC Rouen), Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM), CHU Pontchaillou [Rennes], Centre d'Investigation Clinique [Rennes] (CIC), Université de Rennes (UR)-Hôpital Pontchaillou-Institut National de la Santé et de la Recherche Médicale (INSERM), CHU Saint-Antoine [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Hôpital Louis Mourier - AP-HP [Colombes], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Hôpital Haut-Lévêque [CHU Bordeaux], CHU Bordeaux [Bordeaux], Service d'Hépato-Gastroentérologie [CHU Rouen], and Service de Colo-proctologie, Clinique des Cèdres, Cornebarrieu, France
Subjects: Oncology, [SDV]Life Sciences [q-bio], Gastroenterology
Published: 2023
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4. Adenoma detection with blue-water infusion colonoscopy: a randomized trial

Author: Marc O’Brien, Olivia Scalone, D. Pere-Verge, Sandrine Baubet, Claire Billioud, Karine Stroeymeyt-Martin, Driffa Moussata, Christine Chambon-Augoyard, Guillermo Aguero Garcete, Stéphane Degeorges, Sandrine Touzet, Adriane Lesne, Thierry Ponchon, Jérôme Rivory, Audrey Pasquion, Claire Cruiziat, Sylvaine Chalumeau, Florence Léger-Nguyen, S. Poupon-Bourdy, Jean-Marc Phelip, Philippe Brulet, Benjamin Hamel, J. C. Souquet, Thierry Fontanges, Laurent Magaud, Fabien Petit-Laurent, Julien Scanzi, Laurent Poincloux, Angélique Denis, Marion Chauvenet, Mathieu Pioche, Olivier Rouquette, Gwenaelle Tourlonias, Elsa Thimonier, Emmanuelle Graillot, CHU Estaing [Clermont-Ferrand], Centre Hospitalier Lyon Sud [CHU - HCL] ( CHLS ), Hospices Civils de Lyon ( HCL ), Gastro-enterology department, CHU Clermont-Ferrand, Hepato-Gastroenterology, Hospices Civils de Lyon ( HCL ) -Hospices Civils de Lyon ( HCL ), Lipides - Nutrition - Cancer (U866) ( LNC ), Université de Bourgogne ( UB ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon ( ENSBANA ), Service d'hépato-gastro-entérologie, CH Pierre Oudot Bourgoin-Jallieu, Hôpital de la Croix-Rousse [CHU - HCL], Hôpital Villefranche sur Saône, Université Panthéon-Sorbonne ( UP1 ), Département de Gastroentérologie et hépatologie, Université Joseph Fourier - Grenoble 1 ( UJF ) -CHU Grenoble-Hôpital Michallon, Institut Pascal - Clermont Auvergne ( IP ), Sigma CLERMONT ( Sigma CLERMONT ) -Université Clermont Auvergne ( UCA ) -Centre National de la Recherche Scientifique ( CNRS ), Hôpital Edouard Herriot [CHU - HCL], Application des ultrasons à la thérapie ( LabTAU ), Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale ( INSERM ), Centre Hospitalier Lyon Sud [CHU - HCL] (CHLS), Hospices Civils de Lyon (HCL), Hospices Civils de Lyon (HCL)-Hospices Civils de Lyon (HCL), Université Paris 1 Panthéon-Sorbonne (UP1), Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble-Hôpital Michallon, Institut Pascal (IP), SIGMA Clermont (SIGMA Clermont)-Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Application des ultrasons à la thérapie (LabTAU), Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre Léon Bérard [Lyon]-Institut National de la Santé et de la Recherche Médicale (INSERM), and SIGMA Clermont (SIGMA Clermont)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS)
Subjects: Adenoma, Male, medicine.medical_specialty, medicine.medical_treatment, Operative Time, Color, Colonoscopy, Adenocarcinoma, Gastroenterology, law.invention, 03 medical and health sciences, 0302 clinical medicine, Randomized controlled trial, law, Internal medicine, medicine, Humans, Intubation, Cecum, Intubation, Gastrointestinal, ComputingMilieux_MISCELLANEOUS, Aged, medicine.diagnostic_test, business.industry, Fecal occult blood, Water, [SDV.MHEP.HEG]Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology, Odds ratio, Middle Aged, medicine.disease, Confidence interval, 3. Good health, Clinical trial, 030220 oncology & carcinogenesis, Female, 030211 gastroenterology & hepatology, [ SDV.MHEP.HEG ] Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology, Colorectal Neoplasms, business
Abstract: Background and aims Colonoscopy is currently the reference method to detect colorectal neoplasia, yet some adenomas remain undetected. The water infusion technique and dying with indigo carmine has shown interesting results for reducing this miss rate. The aim of this study was to compare the adenoma detection rate (adenoma and adenocarcinoma; ADR) and the mean number of adenomas per patient (MAP) for blue-water infusion colonoscopy (BWIC) versus standard colonoscopy. Methods We performed a multicenter, randomized controlled trial in eight units, including patients with a validated indication for colonoscopy (symptoms, familial or personal history, fecal occult blood test positive). Consenting patients were randomized 1:1 to BWIC or standard colonoscopy. All colonoscopies were performed by experienced colonoscopists. All colonoscopy quality indicators were prospectively recorded. Results Among the 1065 patients included, colonoscopies were performed completely for 983 patients (514 men; mean age 59.1). The ADR was not significantly different between the groups; 40.4 % in the BWIC group versus 37.5 % in the standard colonoscopy group (odds ratio [OR] 1.13; 95 % confidence interval [CI] 0.87 – 1.48; P = 0.35). MAP was significantly greater in the BWIC group (0.79) than in the standard colonoscopy group (0.64; P = 0.005). For advanced adenomas, the results were 50 (10.2 %) and 36 (7.3 %), respectively (P = 0.10). The cecal intubation rate was not different but the time to cecal intubation was significantly longer in BWIC group (9.9 versus 6.2 minutes; P Conclusion Despite the higher MAP with BWIC, the routine use of BWIC does not translate to a higher ADR. Whether increased detection ultimately results in a lower rate of interval carcinoma is not yet known.Clinical trials registration: EudraCT 2012-A00548 – 35; NCT01937429.
Published: 2017
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5. Farnesoid X receptor alpha ligands inhibit HDV in vitro replication and virion infectivity

Author: Legrand, Anne-Flore, Lucifora, Julie, Lacombe, Benoît, Ménard, Camille, Michelet, Maud, Foca, Adrien, Abrial, Pauline, Salvetti, Anna, Rivoire, Michel, Lotteau, Vincent, Durantel, David, André, Patrice, Ramière, Christophe, Centre International de Recherche en Infectiologie (CIRI), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre de Recherche en Cancérologie de Lyon (UNICANCER/CRCL), Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Application des ultrasons à la thérapie (LabTAU), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire de Virologie [HCL-Hôpital de la Croix Rousse], Hôpital de la Croix-Rousse [CHU - HCL], and Hospices Civils de Lyon (HCL)-Hospices Civils de Lyon (HCL)
Subjects: bile acids, FXR, Hepatology, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, HDV, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, antiviral activity, hepatocytes, [SDV.MHEP.HEG]Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology
Abstract: International audience; Background and Aims: HDV, a satellite of HBV, is responsible for the most severe form of human viral hepatitis, for which curative therapy is still awaited. Both HBV and HDV use the hepatic transporter of bile acids (ie, Na+-taurocholate cotransporting polypeptide) to enter hepatocytes. We have previously shown that ligands of the farnesoid-X-receptor alpha (FXR), a master regulator of bile acids metabolism, inhibit HBV replication. Here we asked whether FXR ligands can also control HDV infection.Approach and Results: In vitro HDV monoinfections or HDV/HBV coinfections and superinfections were performed in differentiated HepaRG cells (dHepaRG) and primary human hepatocytes. Following treatment with FXR ligands, HDV RNAs and antigens were analyzed by RT-qPCR, northern blot, immunofluorescence, and western blot. Virus secretion was studied by RNA quantification in supernatants, and the infectivity of secreted HDV particles was measured by reinfection of naive HuH7.5-Na+-taurocholate cotransporting polypeptide cells. In HDV/HBV superinfection models, a 10-day treatment with FXR ligand GW4064 decreased intracellular HDV RNAs by 60% and 40% in dHepaRG cells and primary human hepatocytes, respectively. Both HDV genomic and antigenomic RNAs were affected by treatment, which also reduced the amount of intracellular delta antigen. This antiviral effect was also observed in HDV monoinfected dHepaRG cells, abolished by FXR loss of function, and reproduced with other FXR ligands. In HBV/HDV coinfected dHepaRG cells, HDV secretion was decreased by 60% and virion-specific infectivity by >95%.Conclusions: FXR ligands both inhibit directly (ie, independently of anti-HBV activity) and indirectly (ie, dependently of anti-HBV activity) the replication, secretion, and infectivity of HDV. The overall anti-HDV activity was superior to that obtained with interferon-α, highlighting the therapeutic potential of FXR ligands in HDV-infected patients.
Published: 2023
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6. Transfer Learning for Prostate Cancer Mapping Based on Multicentric MR Imaging Databases

Author: Rahaf Aljundi, Carole Lartizien, Olivier Rouvière, Fabrice Prost-Boucle, Jérôme Lehaire, 2 - Images et Modèles, Centre de Recherche en Acquisition et Traitement de l'Image pour la Santé ( CREATIS ), Hospices Civils de Lyon ( HCL ) -Université Jean Monnet [Saint-Étienne] ( UJM ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ) -Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon ( INSA Lyon ), Université de Lyon-Institut National des Sciences Appliquées ( INSA ) -Institut National des Sciences Appliquées ( INSA ) -Hospices Civils de Lyon ( HCL ) -Université Jean Monnet [Saint-Étienne] ( UJM ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ) -Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Institut National des Sciences Appliquées ( INSA ) -Institut National des Sciences Appliquées ( INSA ), Application des ultrasons à la thérapie ( LabTAU ), Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Centre Léon Bérard [Lyon]-Institut National de la Santé et de la Recherche Médicale ( INSERM ), Images et Modèles, Centre de Recherche en Acquisition et Traitement de l'Image pour la Santé (CREATIS), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Hospices Civils de Lyon (HCL)-Université Jean Monnet [Saint-Étienne] (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Hospices Civils de Lyon (HCL)-Université Jean Monnet [Saint-Étienne] (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Application des ultrasons à la thérapie (LabTAU), Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: Database, [SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/Imaging, Computer science, business.industry, [SDV.CAN]Life Sciences [q-bio]/Cancer, Context (language use), computer.software_genre, Machine learning, medicine.disease, Field (computer science), Transfer learning, [ SDV.CAN ] Life Sciences [q-bio]/Cancer, Prostate cancer, Prostate cancer screening, ComputingMethodologies_PATTERNRECOGNITION, medicine, Data mining, Artificial intelligence, Computer-aided detection system, business, Transfer of learning, computer, Subspace topology, [ SDV.IB.IMA ] Life Sciences [q-bio]/Bioengineering/Imaging
Abstract: International audience; This paper addresses the issue of fusing datasets coming from different imaging protocols or scanners to boost the performance of computer aided diagnostic system. We present novel contributions in the field of subspace alignment methods that are part of domain adaptation framework. We first introduce a simple approach based on scaling the features of the different distribution and accounting for the class information. We also extend an unsupervised landmark based approach that has been recently developed to the supervised setting. These methods are evaluated in the context of prostate cancer screening based on two patient MRI databases acquired on different scanners. We demonstrate promising performance of the scaling based method when both databases contain similar number of annotated samples, and stable performance of the landmark based method even with unbalanced datasets.
Published: 2015
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7. Evaluation of Eventual Risks of Unseeded Inertial Cavitation for Enhancing the Delivery of Chemotherapies

Author: Lafond , Maxime, Mestas , Jean-Louis, Prieur , Fabrice, Chettab , Kamel, Clézardin , Philippe, Lafon , Cyril, Application des ultrasons à la thérapie (LabTAU), Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM), Caviskills SAS, Centre de Recherche en Cancérologie de Lyon (UNICANCER/CRCL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Physiopathologie, diagnostic et traitements des maladies osseuses / Pathophysiology, Diagnosis & Treatments of Bone Diseases (LYOS), Université Claude Bernard Lyon 1 (UCBL), C.L.A.R.A., Caviskills, the LabEx DEVweCAN and LabEx CeLya, Application des ultrasons à la thérapie ( LabTAU ), Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale ( INSERM ), Centre de Recherche en Cancérologie de Lyon ( CRCL ), Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Centre Léon Bérard [Lyon]-Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), Physiopathologie, diagnostic et traitements des maladies osseuses / Pathophysiology, Diagnosis & Treatments of Bone Diseases ( LYOS ), Duchange, Nathalie, and Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)
Subjects: [SDV.IB] Life Sciences [q-bio]/Bioengineering, Therapy monitoring, Ultrasound, Biophysics, [SDV.IB]Life Sciences [q-bio]/Bioengineering, [ SDV.IB ] Life Sciences [q-bio]/Bioengineering
Abstract: National audience; Acoustic cavitation is widely used to improve drug delivery in tumors. In this study, we evaluate the safety of using unseeded inertial cavitation (without external nucleation agents) for this application. We show that unseeded inertial cavitation (UIC) does not degrade doxorubicin molecules or alter its cytotoxicity. Moreover, histopathologic analysis shows only slight or reversible moderate effects on healthy tissues. Finally, UIC does not promote metastatic spreading. Thus, the particular conditions used in this study provide the possibility of safe UIC concerning these three points.
Published: 2015

8. Development of Targeted Microbubbles for Ultrasound Molecular Imaging of Breast Cancer

Author: Wischhusen, Jennifer, Delcros, Jean-Guy, Mehlen, Patrick, Padilla, Frédéric, Application des ultrasons à la thérapie (LabTAU), Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM), Développement Cancer et Thérapies Ciblées [Lyon] (LabEx DEVweCAN), Université de Lyon, Centre de Recherche en Cancérologie de Lyon (UNICANCER/CRCL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Congrès sous l’égide de la Société Française de Génie Biologique et Médical (SFGBM)., Frouin, Frédérique, Application des ultrasons à la thérapie ( LabTAU ), Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Centre Léon Bérard [Lyon]-Institut National de la Santé et de la Recherche Médicale ( INSERM ), Développement Cancer et Thérapies Ciblées ( LabEx DEVweCAN ), Université de Lyon-Centre de Recherche en Cancérologie, Centre de Recherche en Cancérologie de Lyon ( CRCL ), Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), and Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: [SDV.IB] Life Sciences [q-bio]/Bioengineering, animal structures, nervous system, embryonic structures, fungi, Ultrasound, [SDV.IB]Life Sciences [q-bio]/Bioengineering, [ SDV.IB ] Life Sciences [q-bio]/Bioengineering, Contrast agents, Molecular Imaging
Abstract: National audience; A subtype of metastatic breast cancer has recently been identified, in which netrin-1 is over-expressed. Netrin-1 binds to dependence receptors inducing survival, while its absence actively triggers apoptosis. An anti-netrin-1 therapy is under development , which disrupts ligand-receptor interaction and induces apoptosis. To identify potential responders for such a targeted therapy, netrin-1 expression has to be analyzed. Molecular imaging with ultrasound (US) is able to detect the expression of a specific protein when using targeted contrast agents. Thus, we aim to develop an US molecular imaging approach using microbubbles (MB) functionalized with an anti-netrin-1 antibody. Binding was tested on purified netrin-1 protein in static and dynamic conditions. Anti-netrin-1 MB were validated in in-vitro assays and will be further tested in pre-clinical studies using metastatic breast cancer animal models.
Published: 2015

9. Hepatitis D virus interferes with hepatitis B virus RNA production via interferon-dependent and -independent mechanisms

Author: Julie Lucifora, Dulce Alfaiate, Caroline Pons, Maud Michelet, Ricardo Ramirez, Floriane Fusil, Fouzia Amirache, Axel Rossi, Anne-Flore Legrand, Emilie Charles, Serena Vegna, Rayan Farhat, Michel Rivoire, Guillaume Passot, Nicolas Gadot, Barbara Testoni, Charlotte Bach, Thomas F. Baumert, Anastasia Hyrina, Rudolf K. Beran, Fabien Zoulim, Andre Boonstra, Hildegard Büning, Eloi R. Verrier, François-Loïc Cosset, Simon P. Fletcher, Anna Salvetti, David Durantel, Centre International de Recherche en Infectiologie (CIRI), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut des Agents Infectieux [Lyon] (IAI), Hospices Civils de Lyon (HCL), Centre de Recherche en Cancérologie de Lyon (UNICANCER/CRCL), Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Gilead Sciences, Inc. [Foster City, CA, USA], Virus enveloppés, vecteurs et immunothérapie – Enveloped viruses, Vectors and Immuno-therapy (EVIR), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Institute of Experimental Hematology [Hannover, Germany], Hannover Medical School [Hannover] (MHH), Application des ultrasons à la thérapie (LabTAU), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM), Service d'Oncologie Médicale [Centre hospitalier Lyon Sud - HCL], Centre Hospitalier Lyon Sud [CHU - HCL] (CHLS), Hospices Civils de Lyon (HCL)-Hospices Civils de Lyon (HCL), Centre pour l'innovation en cancérologie de Lyon (CICLY), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon, Centre Léon Bérard [Lyon], Institut de Recherche sur les Maladies Virales et Hépatiques (IVH), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Hôpital de la Croix-Rousse [CHU - HCL], Gastroenterology and Hepatology, Erasmus University Medical Center [Rotterdam] (Erasmus MC), German Center for Infection Research - partner site Hannover-Braunschweig (DZIF), and Gastroenterology & Hepatology
Subjects: Hepatology, SDG 3 - Good Health and Well-being, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, [SDV.MHEP.HEG]Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology, Sciences du Vivant [q-bio]/Médecine humaine et pathologie
Abstract: Background & Aims: Chronic coinfection with HBV and HDV leads to the most aggressive form of chronic viral hepatitis. Herein, we aimed to elucidate the molecular mechanisms underlying the widely reported observation that HDV interferes with HBV in most coinfected patients. Methods: Patient liver tissues, primary human hepatocytes, HepaRG cells and human liver chimeric mice were used to analyze the effect of HDV on HBV using virological and RNA-sequencing analyses, as well as RNA synthesis, stability and association assays. Results: Transcriptomic analyses in cell culture and mouse models of coinfection enabled us to define an HDV-induced signature, mainly composed of interferon (IFN)-stimulated genes (ISGs). We also provide evidence that ISGs are upregulated in chronically HDV/HBV-coinfected patients but not in cells that only express HDV antigen (HDAg). Inhibition of the hepatocyte IFN response partially rescued the levels of HBV parameters. We observed less HBV RNA synthesis upon HDV infection or HDV protein expression. Additionally, HDV infection or expression of HDAg alone specifically accelerated the decay of HBV RNA, and HDAg was associated with HBV RNAs. On the contrary, HDAg expression did not affect other viruses such as HCV or SARS-CoV-2. Conclusions: Our data indicate that HDV interferes with HBV through both IFN-dependent and IFN-independent mechanisms. Specifically, we uncover a new viral interference mechanism in which proteins of a satellite virus affect the RNA production of its helper virus. Exploiting these findings could pave the way to the development of new therapeutic strategies against HBV. Impact and implications: Although the molecular mechanisms remained unexplored, it has long been known that despite its dependency, HDV decreases HBV viremia in patients. Herein, using in vitro and in vivo models, we showed that HDV interferes with HBV through both IFN-dependent and IFN-independent mechanisms affecting HBV RNA metabolism, and we defined the HDV-induced modulation signature. The mechanisms we uncovered could pave the way for the development of new therapeutic strategies against HBV by mimicking and/or increasing the effect of HDAg on HBV RNA. Additionally, the HDV-induced modulation signature could potentially be correlated with responsiveness to IFN-α treatment, thereby helping to guide management of HBV/HDV-coinfected patients.
Published: 2023
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10. Computer-aided diagnostic system for prostate cancer detection and characterization combining learned dictionaries and supervised classification

Author: Rémi Flamary, Carole Lartizien, Jérôme Lehaire, Olivier Rouvière, 2 - Images et Modèles, Centre de Recherche en Acquisition et Traitement de l'Image pour la Santé ( CREATIS ), Hospices Civils de Lyon ( HCL ) -Université Jean Monnet [Saint-Étienne] ( UJM ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ) -Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon ( INSA Lyon ), Université de Lyon-Institut National des Sciences Appliquées ( INSA ) -Institut National des Sciences Appliquées ( INSA ) -Hospices Civils de Lyon ( HCL ) -Université Jean Monnet [Saint-Étienne] ( UJM ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ) -Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Institut National des Sciences Appliquées ( INSA ) -Institut National des Sciences Appliquées ( INSA ), Joseph Louis LAGRANGE ( LAGRANGE ), Université Nice Sophia Antipolis ( UNS ), Université Côte d'Azur ( UCA ) -Université Côte d'Azur ( UCA ) -Observatoire de la Côte d'Azur, Université Côte d'Azur ( UCA ) -Centre National de la Recherche Scientifique ( CNRS ), Application des ultrasons à la thérapie ( LabTAU ), Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Centre Léon Bérard [Lyon]-Institut National de la Santé et de la Recherche Médicale ( INSERM ), Images et Modèles, Centre de Recherche en Acquisition et Traitement de l'Image pour la Santé (CREATIS), Université Jean Monnet [Saint-Étienne] (UJM)-Hospices Civils de Lyon (HCL)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Jean Monnet [Saint-Étienne] (UJM)-Hospices Civils de Lyon (HCL)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Joseph Louis LAGRANGE (LAGRANGE), Université Côte d'Azur (UCA)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Observatoire de la Côte d'Azur, Université Côte d'Azur (UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Application des ultrasons à la thérapie (LabTAU), Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Centre Léon Bérard [Lyon]-Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: Ground truth, medicine.diagnostic_test, [ INFO.INFO-IM ] Computer Science [cs]/Medical Imaging, Computer science, business.industry, Feature extraction, Cancer, Pattern recognition, Magnetic resonance imaging, Sparse approximation, medicine.disease, computer.software_genre, Logistic regression, Machine learning, Support vector machine, Prostate cancer, Binary classification, Voxel, medicine, Computer-aided, [INFO.INFO-IM]Computer Science [cs]/Medical Imaging, Artificial intelligence, business, computer
Abstract: International audience; This paper aims at presenting results of a computer-aided diagnostic (CAD) system for voxel based detection and characterization of prostate cancer in the peripheral zone based on multiparametric magnetic resonance (mp-MR) imaging. We propose an original scheme with the combination of a feature extraction step based on a sparse dictionary learning (DL) method and a supervised classification in order to discriminate normal {N}, normal but suspect {NS} tissues as well as different classes of cancer tissue whose aggressiveness is characterized by the Gleason score ranging from 6 {GL6} to 9 {GL9}. We compare the classification performance of two supervised methods, the linear support vector machine (SVM) and the logistic regression (LR) classifiers in a binary classification task. Classification performances were evaluated over an mp-MR image database of 35 patients where each voxel was labeled, based on a ground truth, by an expert radiologist. Results show that the proposed method in addition to being explicable thanks to the sparse representation of the voxels compares well (AUC>0.8) with recent state-of-the-art performances. Preliminary visual analysis of example patient cancer probability maps indicate that cancer probabilities tend to increase as a function of the Gleason score.
Published: 2014
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11. SVM with feature selection and smooth prediction in images: Application to CAD of prostate cancer

Author: Alain Rakotomamonjy, Rémi Flamary, Emilie Niaf, Carole Lartizien, Olivier Rouvière, Centre de Recherche en Acquisition et Traitement de l'Image pour la Santé ( CREATIS ), Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon ( INSA Lyon ), Université de Lyon-Institut National des Sciences Appliquées ( INSA ) -Institut National des Sciences Appliquées ( INSA ) -Hospices Civils de Lyon ( HCL ) -Université Jean Monnet [Saint-Étienne] ( UJM ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), Application des ultrasons à la thérapie ( LabTAU ), Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale ( INSERM ), A*Star-Nus, Clinical Imaging Research Centre, Joseph Louis LAGRANGE ( LAGRANGE ), Université Nice Sophia Antipolis ( UNS ), Université Côte d'Azur ( UCA ) -Université Côte d'Azur ( UCA ) -Observatoire de la Côte d'Azur, Université Côte d'Azur ( UCA ) -Centre National de la Recherche Scientifique ( CNRS ), Laboratoire d'Informatique, de Traitement de l'Information et des Systèmes ( LITIS ), Université Le Havre Normandie ( ULH ), Normandie Université ( NU ) -Normandie Université ( NU ) -Université de Rouen Normandie ( UNIROUEN ), Normandie Université ( NU ) -Institut national des sciences appliquées Rouen Normandie ( INSA Rouen Normandie ), Normandie Université ( NU ), 2 - Images et Modèles, Université de Lyon-Institut National des Sciences Appliquées ( INSA ) -Institut National des Sciences Appliquées ( INSA ) -Hospices Civils de Lyon ( HCL ) -Université Jean Monnet [Saint-Étienne] ( UJM ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ) -Université Claude Bernard Lyon 1 ( UCBL ), Centre de Recherche en Acquisition et Traitement de l'Image pour la Santé (CREATIS), Université Jean Monnet [Saint-Étienne] (UJM)-Hospices Civils de Lyon (HCL)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Application des ultrasons à la thérapie (LabTAU), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre Léon Bérard [Lyon]-Institut National de la Santé et de la Recherche Médicale (INSERM), Joseph Louis LAGRANGE (LAGRANGE), Université Côte d'Azur (UCA)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Observatoire de la Côte d'Azur, Université Côte d'Azur (UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'Informatique, de Traitement de l'Information et des Systèmes (LITIS), Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Université Le Havre Normandie (ULH), Normandie Université (NU), Images et Modèles, and Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Jean Monnet [Saint-Étienne] (UJM)-Hospices Civils de Lyon (HCL)-Institut National des Sciences Appliquées de Lyon (INSA Lyon)
Subjects: Optimization problem, Computer science, [SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/Imaging, ComputingMethodologies_IMAGEPROCESSINGANDCOMPUTERVISION, Feature selection, [ SPI.SIGNAL ] Engineering Sciences [physics]/Signal and Image processing, computer.software_genre, Machine learning, Malignancy, Regularization (mathematics), Prostate cancer, Voxel, medicine, [ SDV.IB.IMA ] Life Sciences [q-bio]/Bioengineering/Imaging, medicine.diagnostic_test, Structured support vector machine, business.industry, Magnetic resonance imaging, Pattern recognition, medicine.disease, Linear discriminant analysis, Support vector machine, ComputingMethodologies_PATTERNRECOGNITION, Norm (mathematics), Artificial intelligence, business, computer, Classifier (UML), [SPI.SIGNAL]Engineering Sciences [physics]/Signal and Image processing
Abstract: International audience; We propose a new computer-aided detection scheme for prostate cancer screening on multiparametric magnetic resonance (mp-MR) images. Based on an annotated training database of mp-MR images from thirty patients, we train a novel support vector machine (SVM)-inspired classifier which simultaneously learns an optimal linear discriminant and a subset of predictor variables (or features) that are most relevant to the classification task, while promoting spatial smoothness of the malignancy prediction maps. The approach uses a ℓ1-norm in the regularization term of the optimization problem that rewards sparsity. Spatial smoothness is promoted via an additional cost term that encodes the spatial neighborhood of the voxels, to avoid noisy prediction maps. Experimental comparisons of the proposed ℓ1-Smooth SVM scheme to the regular ℓ2-SVM scheme demonstrate a clear visual and numerical gain on our clinical dataset.
Published: 2014
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12. Prostate Focal Peripheral Zone Lesions: Characterization at Multiparametric MR Imaging--Influence of a Computer-aided Diagnosis System

Author: Emilie Niaf, Florence Mège-Lechevallier, Muriel Rabilloud, Flavie Bratan, Carole Lartizien, Olivier Rouvière, Laurent Roche, Centre de Recherche en Acquisition et Traitement de l'Image pour la Santé ( CREATIS ), Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon ( INSA Lyon ), Université de Lyon-Institut National des Sciences Appliquées ( INSA ) -Institut National des Sciences Appliquées ( INSA ) -Hospices Civils de Lyon ( HCL ) -Université Jean Monnet [Saint-Étienne] ( UJM ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), Application des ultrasons à la thérapie ( LabTAU ), Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale ( INSERM ), 2 - Images et Modèles, Université de Lyon-Institut National des Sciences Appliquées ( INSA ) -Institut National des Sciences Appliquées ( INSA ) -Hospices Civils de Lyon ( HCL ) -Université Jean Monnet [Saint-Étienne] ( UJM ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ) -Université Claude Bernard Lyon 1 ( UCBL ), Département de médecine nucléaire, Hospices Civils de Lyon ( HCL ), Laboratoire de Biométrie et Biologie Evolutive ( LBBE ), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique ( Inria ) -Centre National de la Recherche Scientifique ( CNRS ), Department of Urinary and Vascular Radiology, Centre de Recherche en Acquisition et Traitement de l'Image pour la Santé (CREATIS), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Hospices Civils de Lyon (HCL)-Université Jean Monnet [Saint-Étienne] (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Application des ultrasons à la thérapie (LabTAU), Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM), Images et Modèles, Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Hospices Civils de Lyon (HCL)-Université Jean Monnet [Saint-Étienne] (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Hospices Civils de Lyon (HCL), Laboratoire de Biométrie et Biologie Evolutive - UMR 5558 (LBBE), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS), Biostatistiques santé, Département biostatistiques et modélisation pour la santé et l'environnement [LBBE], Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Laboratoire de Biométrie et Biologie Evolutive - UMR 5558 (LBBE), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), and Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL)
Subjects: Male, medicine.medical_specialty, medicine.medical_treatment, Contrast Media, [ SPI.SIGNAL ] Engineering Sciences [physics]/Signal and Image processing, Diagnosis, Differential, Meglumine, Prostate, Area under curve, Organometallic Compounds, Humans, Medicine, Radiology, Nuclear Medicine and imaging, Diagnosis, Computer-Assisted, Prospective Studies, Aged, Observer Variation, Prostatectomy, business.industry, Prostatic Neoplasms, Middle Aged, Prostate-Specific Antigen, Magnetic Resonance Imaging, Mr imaging, Peripheral zone, Diffusion Magnetic Resonance Imaging, medicine.anatomical_structure, ROC Curve, Computer-aided diagnosis, Area Under Curve, Preoperative Period, Radiology, Observer variation, business, [SPI.SIGNAL]Engineering Sciences [physics]/Signal and Image processing
Abstract: International audience; Purpose To assess the impact of a computer-aided diagnosis (CAD) system in the characterization of focal prostate lesions at multiparametric magnetic resonance (MR) imaging. Materials and Methods Formal institutional review board approval was not required. Thirty consecutive 1.5-T multiparametric MR imaging studies (with T2-weighted, diffusion-weighted, and dynamic contrast material-enhanced imaging) obtained before radical prostatectomy in patients between September 2008 and February 2010 were reviewed. Twelve readers assessed the likelihood of malignancy of 88 predefined peripheral zone lesions by using a five-level (level, 0-4) subjective score (SS) in reading session 1. This was repeated 5 weeks later in reading session 2. The CAD results were then disclosed, and in reading session 3, the readers could amend the scores assigned during reading session 2. Diagnostic accuracy was assessed by using a receiver operating characteristic (ROC) regression model and was quantified with the area under the ROC curve (AUC). Results Mean AUCs were significantly lower for less experienced (
Published: 2014
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13. Correction: Endoscopic treatment of large gastric leaks after gastrectomy using the combination of double pigtail drains crossing a covered stent

Author: Clara Yzet, Sami Hakim, Mathieu Pioche, Jean-Phillippe Le Mouel, Constance Deschepper, Pierre Lafeuille, Richard Delcenserie, Thierry Yzet, Eric Nguyen-Khac, Mathurin Fumery, Franck Brazier, CHU Amiens-Picardie, Simplification des soins chez les patients complexes - UR UPJV 7518 (SSPC), Université de Picardie Jules Verne (UPJV), Service d'Hépato Gastroenterologie [CHU Amiens-Picardie], Application des ultrasons à la thérapie (LabTAU), Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pascal (IP), Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne (UCA)-Institut national polytechnique Clermont Auvergne (INP Clermont Auvergne), Université Clermont Auvergne (UCA)-Université Clermont Auvergne (UCA), Groupe de Recherche sur l'alcool et les pharmacodépendances - UMR INSERM_S 1247 (GRAP), Université de Picardie Jules Verne (UPJV)-Institut National de la Santé et de la Recherche Médicale (INSERM), Périnatalité et Risques Toxiques - UMR INERIS_I 1 (PERITOX), and Institut National de l'Environnement Industriel et des Risques (INERIS)-Université de Picardie Jules Verne (UPJV)-CHU Amiens-Picardie
Subjects: Surgery, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Published: 2022
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14. Effect of oral antimicrobial prophylaxis on surgical site infection after elective colorectal surgery:multicentre, randomised, double blind, placebo controlled trial

Author: Emmanuel Futier, Samir Jaber, Matthias Garot, Marie Vignaud, Yves Panis, Karem Slim, Jean-Christophe Lucet, Gilles Lebuffe, Alexandre Ouattara, Younes El Amine, Philippe Couderc, Aurélien Dupré, Audrey De Jong, Sigismond Lasocki, Marc Leone, Julien Pottecher, Bruno Pereira, Catherine Paugam-Burtz, Université de Lille, CHU Lille, Centre Hospitalier Régional Universitaire [Lille] [CHRU Lille], Hôpital Claude Huriez [Lille], Groupe de Recherche sur les formes Injectables et les Technologies Associées (GRITA) - ULR 7365, Génétique, Reproduction et Développement (GReD), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne (UCA), Physiologie & médecine expérimentale du Cœur et des Muscles [U 1046] (PhyMedExp), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM), Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), Infection, Anti-microbiens, Modélisation, Evolution (IAME (UMR_S_1137 / U1137)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité)-Université Sorbonne Paris Nord, Biologie des maladies cardiovasculaires = Biology of Cardiovascular Diseases, Université de Bordeaux (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Application des ultrasons à la thérapie (LabTAU), Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: Adult, Male, [SDV.MHEP.HEG]Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology, General Medicine, [SDV.MHEP.CHI]Life Sciences [q-bio]/Human health and pathology/Surgery, Middle Aged, Antibiotic Prophylaxis, Anti-Bacterial Agents, Ornidazole, Anti-Infective Agents, Double-Blind Method, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, [SDV.SP.PHARMA]Life Sciences [q-bio]/Pharmaceutical sciences/Pharmacology, Humans, Surgical Wound Infection, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, Female, Colorectal Surgery
Abstract: Objective To investigate whether oral antimicrobial prophylaxis as an adjunct to intravenous antibiotic prophylaxis reduces surgical site infections after elective colorectal surgery. Design Multicentre, randomised, double blind, placebo controlled trial. Setting 11 university and non-university hospitals in France between 25 May 2016 and 8 August 2019. Participants 926 adults scheduled for elective colorectal surgery. Intervention Patients were randomised to receive either a single 1 g dose of ornidazole (n=463) or placebo (n=463) orally 12 hours before surgery, in addition to intravenous antimicrobial prophylaxis before surgical incision. Main outcome measures The primary outcome was the proportion of patients with surgical site infection within 30 days after surgery. Secondary outcomes included individual types of surgical site infections and major postoperative complications (Clavien-Dindo classification grade 3 or higher) within 30 days after surgery. Results Of the 960 patients who were enrolled, 926 (96%) were included in the analysis. The mean age of participants was 63 years and 554 (60%) were men. Surgical site infection within 30 days after surgery occurred in 60 of 463 patients (13%) in the oral prophylaxis group and 100 of 463 (22%) in the placebo group (absolute difference −8.6%, 95% confidence interval −13.5% to −3.8%; relative risk 0.60, 95% confidence interval 0.45 to 0.80). The proportion of patients with deep infections was 4.8% in the oral prophylaxis group and 8.0% in the placebo group (absolute difference −3.2%, 95% confidence interval −6.4% to −0.1%). The proportion of patients with organ space infections was 5.0% in the oral prophylaxis group and 8.4% in the placebo group (absolute difference −3.4%, −6.7% to −0.2%). Major postoperative complications occurred in 9.1% patients in the oral prophylaxis group and 13.6% in the placebo group (absolute difference −4.5%, −8.6% to −0.5%). Conclusion Among adults undergoing elective colorectal surgery, the addition of a single 1 g dose of ornidazole compared with placebo before surgery significantly reduced surgical site infections. Trial registration ClinicalTrials.gov NCT02618720 .
Published: 2022
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15. Evaluation of a 3D MR/MR elastic registration method for prostate cancer imaging

Author: Lehaire, J., Lartizien, C., Niaf, E., Baumann, M., Souchon, R., Bratan, F., Rouvière, O., 2 - Images et Modèles, Centre de Recherche en Acquisition et Traitement de l'Image pour la Santé ( CREATIS ), Hospices Civils de Lyon ( HCL ) -Université Jean Monnet [Saint-Étienne] ( UJM ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ) -Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon ( INSA Lyon ), Université de Lyon-Institut National des Sciences Appliquées ( INSA ) -Institut National des Sciences Appliquées ( INSA ) -Hospices Civils de Lyon ( HCL ) -Université Jean Monnet [Saint-Étienne] ( UJM ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ) -Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Institut National des Sciences Appliquées ( INSA ) -Institut National des Sciences Appliquées ( INSA ), Application des ultrasons à la thérapie ( LabTAU ), Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Centre Léon Bérard [Lyon]-Institut National de la Santé et de la Recherche Médicale ( INSERM ), Société KOELIS, entreprise, Department of Urinary and Vascular Radiology, Hospices Civils de Lyon ( HCL ), Images et Modèles, Centre de Recherche en Acquisition et Traitement de l'Image pour la Santé (CREATIS), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Hospices Civils de Lyon (HCL)-Université Jean Monnet [Saint-Étienne] (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Hospices Civils de Lyon (HCL)-Université Jean Monnet [Saint-Étienne] (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Application des ultrasons à la thérapie (LabTAU), Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM), and Hospices Civils de Lyon (HCL)
Subjects: [SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/Imaging, [ SPI.SIGNAL ] Engineering Sciences [physics]/Signal and Image processing, [SPI.SIGNAL]Engineering Sciences [physics]/Signal and Image processing, [ SDV.IB.IMA ] Life Sciences [q-bio]/Bioengineering/Imaging
Abstract: International audience; Early detection and localization of prostate cancer is crucial for appropriate patient management. Recent studies suggest that computer-assisted diagnosis systems combining different imaging modalities (MR/MR, MR/US) can improve prostate cancer detection1 and open the wayto targeted biopsies and focal therapy2. Such fusion processes are limited by prostate motion and deformation, particularly at the prostate-rectum interface. A non-rigid multimodal image registration process based on shape statistics has been developed to coregister T2-weighted MR and 3D transrectal ultrasound acquisitions3. Based on the good precision achieved4, this method was adapted to coregister T2-weighted, diffusion-weighted (DW) and dynamic contrast-enhanced (DCE) images and evaluated on 28 prostate cancer patients.
Published: 2013

16. Evaluation clinique d'un système d'aide au diagnostic pour la caractérisation des zones suspectes en IRM multi-paramétrique de prostate

Author: Niaf , E., Lartizien , C., Bratan , F., Roche , L., Rabilloud , M., Mège-Lechevallier , F., Rouvière , O., 2 - Images et Modèles, Centre de Recherche en Acquisition et Traitement de l'Image pour la Santé ( CREATIS ), Hospices Civils de Lyon ( HCL ) -Université Jean Monnet [Saint-Étienne] ( UJM ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ) -Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon ( INSA Lyon ), Université de Lyon-Institut National des Sciences Appliquées ( INSA ) -Institut National des Sciences Appliquées ( INSA ) -Hospices Civils de Lyon ( HCL ) -Université Jean Monnet [Saint-Étienne] ( UJM ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ) -Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Institut National des Sciences Appliquées ( INSA ) -Institut National des Sciences Appliquées ( INSA ), Application des ultrasons à la thérapie ( LabTAU ), Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Centre Léon Bérard [Lyon]-Institut National de la Santé et de la Recherche Médicale ( INSERM ), Département de médecine nucléaire, Hospices Civils de Lyon ( HCL ), Images et Modèles, Centre de Recherche en Acquisition et Traitement de l'Image pour la Santé (CREATIS), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Hospices Civils de Lyon (HCL)-Université Jean Monnet [Saint-Étienne] (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Hospices Civils de Lyon (HCL)-Université Jean Monnet [Saint-Étienne] (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Application des ultrasons à la thérapie (LabTAU), Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM), and Hospices Civils de Lyon (HCL)
Subjects: [ SPI.SIGNAL ] Engineering Sciences [physics]/Signal and Image processing, [SPI.SIGNAL]Engineering Sciences [physics]/Signal and Image processing, ComputingMilieux_MISCELLANEOUS
Abstract: National audience
Published: 2012

17. Computer-aided diagnosis of prostate cancer in the peripheral zone using multiparametric MRI

Author: Olivier Rouvière, Emilie Niaf, Florence Mège-Lechevallier, Flavie Bratan, Carole Lartizien, Application des ultrasons à la thérapie (LabTAU), Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM), Images et Modèles, Centre de Recherche en Acquisition et Traitement de l'Image pour la Santé (CREATIS), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Hospices Civils de Lyon (HCL)-Université Jean Monnet [Saint-Étienne] (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Hospices Civils de Lyon (HCL)-Université Jean Monnet [Saint-Étienne] (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Department of Urinary and Vascular Radiology, Hospices Civils de Lyon (HCL), Université de Lyon, Application des ultrasons à la thérapie ( LabTAU ), Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Centre Léon Bérard [Lyon]-Institut National de la Santé et de la Recherche Médicale ( INSERM ), 2 - Images et Modèles, Centre de Recherche en Acquisition et Traitement de l'Image pour la Santé ( CREATIS ), Hospices Civils de Lyon ( HCL ) -Université Jean Monnet [Saint-Étienne] ( UJM ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ) -Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon ( INSA Lyon ), Université de Lyon-Institut National des Sciences Appliquées ( INSA ) -Institut National des Sciences Appliquées ( INSA ) -Hospices Civils de Lyon ( HCL ) -Université Jean Monnet [Saint-Étienne] ( UJM ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ) -Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Institut National des Sciences Appliquées ( INSA ) -Institut National des Sciences Appliquées ( INSA ), and Hospices Civils de Lyon ( HCL )
Subjects: Male, Computer science, medicine.medical_treatment, Feature selection, [ SPI.SIGNAL ] Engineering Sciences [physics]/Signal and Image processing, Naive Bayes classifier, Prostate cancer, Prostate, Image Interpretation, Computer-Assisted, medicine, Humans, Radiology, Nuclear Medicine and imaging, Diagnosis, Computer-Assisted, ComputingMilieux_MISCELLANEOUS, Aged, Radiological and Ultrasound Technology, medicine.diagnostic_test, Receiver operating characteristic, Prostatectomy, business.industry, Multiparametric MRI, Cancer, Discriminant Analysis, Prostatic Neoplasms, Pattern recognition, Magnetic resonance imaging, Mutual information, Middle Aged, Linear discriminant analysis, medicine.disease, Magnetic Resonance Imaging, Support vector machine, Radiography, medicine.anatomical_structure, ROC Curve, Computer-aided diagnosis, Artificial intelligence, business, [SPI.SIGNAL]Engineering Sciences [physics]/Signal and Image processing
Abstract: This study evaluated a computer-assisted diagnosis (CADx) system for determining a likelihood measure of prostate cancer presence in the peripheral zone (PZ) based on multiparametric magnetic resonance (MR) imaging, including T2-weighted, diffusion-weighted and dynamic contrast-enhanced MRI at 1.5 T. Based on a feature set derived from grey-level images, including first-order statistics, Haralick features, gradient features, semi-quantitative and quantitative (pharmacokinetic modelling) dynamic parameters, four kinds of classifiers were trained and compared: nonlinear support vector machine (SVM), linear discriminant analysis, k-nearest neighbours and naive Bayes classifiers. A set of feature selection methods based on t-test, mutual information and minimum-redundancy-maximum-relevancy criteria were also compared. The aim was to discriminate between the relevant features as well as to create an efficient classifier using these features. The diagnostic performances of these different CADx schemes were evaluated based on a receiver operating characteristic (ROC) curve analysis. The evaluation database consisted of 30 sets of multiparametric MR images acquired from radical prostatectomy patients. Using histologic sections as the gold standard, both cancer and nonmalignant (but suspicious) tissues were annotated in consensus on all MR images by two radiologists, a histopathologist and a researcher. Benign tissue regions of interest (ROIs) were also delineated in the remaining prostate PZ. This resulted in a series of 42 cancer ROIs, 49 benign but suspicious ROIs and 124 nonsuspicious benign ROIs. From the outputs of all evaluated feature selection methods on the test bench, a restrictive set of about 15 highly informative features coming from all MR sequences was discriminated, thus confirming the validity of the multiparametric approach. Quantitative evaluation of the diagnostic performance yielded a maximal area under the ROC curve (AUC) of 0.89 (0.81-0.94) for the discrimination of the malignant versus nonmalignant tissues and 0.82 (0.73-0.90) for the discrimination of the malignant versus suspicious tissues when combining the t-test feature selection approach with a SVM classifier. A preliminary comparison showed that the optimal CADx scheme mimicked, in terms of AUC, the human experts in differentiating malignant from suspicious tissues, thus demonstrating its potential for assisting cancer identification in the PZ.
Published: 2012
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18. Spatially broad opening of the blood-brain barrier with an unfocused ultrasound transducer in rabbits

Author: Beccaria, Kevin, CANNEY, Michael, GOLDWIRT, Lauriane, FERNANDEZ, Christine, ADAM, Clovis, AUTRET, Gwennhael, Clément, Olivier, MENASCHE, Philippe, Lafon, Cyril, Chapelon, Jean-Yves, Carpentier, Alexandre, Paris-Centre de Recherche Cardiovasculaire (PARCC - UMR-S U970), Université Paris Descartes - Paris 5 (UPD5)-Hôpital Européen Georges Pompidou [APHP] (HEGP), Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Service de radiologie cardio-vasculaire [CHU HEGP], Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Hôpital Européen Georges Pompidou [APHP] (HEGP), Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO), Therapie Cellulaire en Pathologie Cardio-Vasculaire (UMR_S 633), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Descartes - Paris 5 (UPD5), Service de chirurgie cardiaque et vasculaire [CHU HEGP], Application des ultrasons à la thérapie (LabTAU), Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM), Société Française d'Acoustique, Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Hôpital Européen Georges Pompidou [APHP] (HEGP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Européen Georges Pompidou [APHP] (HEGP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO), System, HAL, Paris-Centre de Recherche Cardiovasculaire ( PARCC - U970 ), Hôpital Européen Georges Pompidou [APHP] ( HEGP ) -Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ), Assistance publique - Hôpitaux de Paris (AP-HP)-Hôpital Européen Georges Pompidou [APHP] ( HEGP ), Therapie Cellulaire en Pathologie Cardio-Vasculaire ( UMR_S 633 ), Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Université Paris Descartes - Paris 5 ( UPD5 ), Application des ultrasons à la thérapie ( LabTAU ), Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale ( INSERM ), Hôpital Européen Georges Pompidou [APHP] (HEGP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Centre Léon Bérard [Lyon]-Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: [SPI.ACOU]Engineering Sciences [physics]/Acoustics [physics.class-ph], [SPI.ACOU] Engineering Sciences [physics]/Acoustics [physics.class-ph], Contrast agent, [ SPI.ACOU ] Engineering Sciences [physics]/Acoustics [physics.class-ph], Blood-Brain Barrier, Unfocused ultrasound
Abstract: International audience; The aim of this work was to study the opening of the blood-brain barrier (BBB) over a large volume using an unfocused ultrasound device in the presence of ultrasound contrast agents in rabbits. A mono-element planar 1MHz ultrasound transducer was used to perform burst sonications in 24 healthy New-Zealand white rabbits after craniectomy and during intravenous injection of Sonovue®. The transducer was operated with a pulse repetition frequency of 1Hz, and a range of pulses lengths and in situ acoustic pressures (10-35ms and 0.3-1MPa respectively). Opening of the BBB was observed in contrast-enhanced images in a 4.7T MRI, through blue dye extravasation and with confocal microscopy. Adverse effects were analyzed on histology. A significant BBB opening limited spatially to the extent of the ultrasound field was observed. BBB opening appeared during the sonication and lasted for several hours. Monitoring was possible on MRI sequences as a significant gadolinium contrast enhancement (p
Published: 2012

19. In-vitro and in-vivo siRNA transfection by an ultrasonic confocal device

Author: MESTAS , Jean-Louis, Grandjean , Noémie, Reslan , Lina, Béra , Jean-Christophe, Dumontet , Charles, Lafon , Cyril, System, HAL, Société Française d'Acoustique, Applications des ultrasons à la thérapie, Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale ( INSERM ), Université de Lyon, Centre de Recherche en Cancérologie de Lyon ( CRCL ), Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), Application des ultrasons à la thérapie ( LabTAU ), Université de Lyon-Université de Lyon-Centre Léon Bérard [Lyon]-Institut National de la Santé et de la Recherche Médicale ( INSERM ), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre de Recherche en Cancérologie de Lyon (UNICANCER/CRCL), Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), and Application des ultrasons à la thérapie (LabTAU)
Subjects: [SPI.ACOU]Engineering Sciences [physics]/Acoustics [physics.class-ph], [SPI.ACOU] Engineering Sciences [physics]/Acoustics [physics.class-ph], cavitation, transfection, [ SPI.ACOU ] Engineering Sciences [physics]/Acoustics [physics.class-ph], ultrasound, sonoporation
Abstract: International audience; The internalization of genetic materials, molecules or tracer particles in cells aims at understanding the intracellular mechanisms or can be considered for new therapies. The sonoporation is more and more used for such objective. The goal of this work is to assess the transfection potential of an ultrasonic device which combined 2 focused transducers (1.1MHz; Diameter and radius of curvature: 50mm) placed confocally. In this study in-vitro transfection tests were first carried out. 2ml tubes (Eppendorf) containing 0.5ml of cellular medium (follicular lymphoma RL; RPMI1640+10%SVF; 2x106cells/ml) and 45µl/ml of siRNA (20nMol; Qiagen; Allstar Neg Control) were placed at the device focal point. Transfection and mortality rates were evaluated according to the duration of irradiation (8s to 60s), the pulse repetition rate (≤500Hz) and the duty cycle (0.12 to 1%) for the peak intensity Isppa=10.8kW/cm2. Maximal transfection rates of 60% associated with very low mortality (3) implanted subcutaneously in mouse CB17-SCID were performed. SiRNA (7.5 µg/ml) were injected into the tumor before ultrasonic irradiation (PRF=250Hz; DC=1%; Scanning rate 1mm/s; Isppa=10.8kW/cm2). The transfection rate was determined by flow cytometry after tumor removal and fragmentation. In-vivo transfection rate reached 16% of the studied samples.
Published: 2012

20. The interacting acoustic bubble pair: coupled oscillation, radiation force and streaming

Author: Inserra, C, Regnaut, G, Doinikov, A, Mauger, Cyril, Blanc-Benon, Philippe, Application des ultrasons à la thérapie (LabTAU), Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire de Mecanique des Fluides et d'Acoustique (LMFA), École Centrale de Lyon (ECL), Université de Lyon-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS), and Mauger, Cyril
Subjects: [SPI]Engineering Sciences [physics], [SPI] Engineering Sciences [physics]
Abstract: International audience
Published: 2022

21. Caractérisation ex vivo du changement de la rétrodiffusion ultrasonore lors d'un traitement par ultrasons focalisés

Author: Rohfritsch, Adrien, Barrère, Victor, Franceschini, Émilie, Melodelima, David, Application des ultrasons à la thérapie (LabTAU), Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire de Mécanique et d'Acoustique [Marseille] (LMA ), Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Centre National de la Recherche Scientifique (CNRS), Société Française d'Acoustique, and Laboratoire de Mécanique et d'Acoustique
Subjects: [PHYS.MECA.ACOU]Physics [physics]/Mechanics [physics]/Acoustics [physics.class-ph]
Abstract: Les ultrasons focalisés de haute intensité (\textit{High Intensity Focused Ultrasound}, HIFU)) permettent le traitement de nombreuses pathologies, de manière non-invasive et sans radiation. Le guidage de l'effet thermique induit par HIFU nécessite un suivi temps réel quantitatif. Le développement d'une telle technique reste un enjeu majeur à l'heure actuelle. Dans ce contexte, la technique quantitative ultrasonore (QUS) basée sur la mesure du coefficient de rétrodiffusion (\textit{Back-Scattering Coefficient} , BSC) semble prometteuse pour faire le suivi des changements de microstructure lors de l'ablation thermique HIFU. Le travail présenté ici a pour objectif de quantifier l'évolution des paramètres d'atténuation, de BSC et de microstructure avant et après le traitement thermique d'échantillons hépatiques par HIFU ou bain marie. \\ Des lésions thermiques sur des foies de porc ex vivo ont été réalisées par HIFU avec un transducteur monoélément fonctionnant à 3 MHz, ou par bain marie en immergeant les tissus durant 10 min dans une solution saline chauffée. Une trentaine d'échantillons ont été traités à des températures comprises entre 37 et 80°C. L'élévation en température a été mesurée grâce à un thermocouple, et le BSC calculé par la technique de fantôme de référence, puis comparé aux prédictions de modèles analytiques basés sur un modèle gaussien ou un modèle de facteur de structure. \\ Dans le cas de lésions créées par HIFU, une relation quasi linéaire de +8 dB entre la température et le BSC jusqu'à environ 75°C est mise en évidence. Pour les échantillons chauffés au bain marie, les mesures ultrasonores jusqu'à 80 MHz permettent d'estimer les caractéristiques (taille et concentration) des sources acoustiques à l'origine des changements de rétrodiffusion observés, et sont corrélés aux microstructures observées sur les coupes histologiques. L'ensemble des résultats présentés permet une meilleure compréhension de l'évolution de la rétrodiffusion ultrasonore des tissus hépatiques lors des traitements HIFU, ouvrant la voie au guidage par imagerie QUS.
Published: 2022

22. Fast and Selective Ablation of Liver Tumors by High-Intensity Focused Ultrasound Using a Toroidal Transducer Guided by Ultrasound Imaging: The Results of Animal Experiments

Author: W. Apoutou N'Djin, Amélie Battais, Victor Barrere, M. Rivoire, Aurélien Dupré, David Melodelima, Application des ultrasons à la thérapie (LabTAU), Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM), and CCSD, Accord Elsevier
Subjects: Time Factors, Liver tumor, Acoustics and Ultrasonics, [SDV]Life Sciences [q-bio], medicine.medical_treatment, Biophysics, 030218 nuclear medicine & medical imaging, Metastasis, Gross examination, 03 medical and health sciences, 0302 clinical medicine, medicine, Animals, Radiology, Nuclear Medicine and imaging, Ultrasonography, Interventional, Radiological and Ultrasound Technology, business.industry, Liver Neoplasms, Ultrasound, medicine.disease, Ablation, High-intensity focused ultrasound, 3. Good health, [SDV] Life Sciences [q-bio], Disease Models, Animal, Transducer, Liver Lobe, 030220 oncology & carcinogenesis, High-Intensity Focused Ultrasound Ablation, Rabbits, Nuclear medicine, business
Abstract: This study demonstrated that high-intensity focused ultrasound (HIFU) produced with an intra-operative toroidal-shaped transducer causes fast, selective liver tumor ablations in an animal model. The HIFU device is composed of 256 emitters working at 3 MHz. A 7.5 MHz ultrasound imaging probe centered on the HIFU transducer guided treatment. VX2 tumor segments (25 mg) were implanted into the right lateral liver lobes of 45 New Zealand rabbits. The animals were evenly divided into groups 1 (toroidal HIFU ablation), 2 (surgical resection) and 3 (untreated control). Therapeutic responses were evaluated with gross pathology and histology 11 d post-treatment. Toroidal transducer-produced HIFU ablation (average ablation rate 10.5 cc/min) allowed fast and homogeneous tumor treatment. Sonograms showed all ablations. VX2 tumors were completely coagulated and surrounded by safety margins without surrounding-organ secondary HIFU lesions. HIFU group tumor volumes at autopsy (39 mm3) were significantly lower than control group volumes (2610 mm3, p < 0.0001). HIFU group tumor metastasis (27%) was lower than resected (33%) and control (67%) group metastasis. Ultrasound imaging, gross pathology and histology results supported these outcomes. HIFU procedures had no complications. Rabbit liver tumor ablation using a toroidal HIFU transducer under ultrasound imaging guidance might therefore be an effective intra-operative treatment for localized liver metastases.
Published: 2020
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23. Evaluation of the Uncertainty of Passive Cavitation Measurements for Blood–Brain Barrier Disruption Monitoring

Author: Cyril Lafon, Nicolas Asquier, Jean-Yves Chapelon, Application des ultrasons à la thérapie (LabTAU), Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM), and CCSD, Accord Elsevier
Subjects: Materials science, Acoustics and Ultrasonics, Swine, Ultrasonography, Doppler, Transcranial, [SDV]Life Sciences [q-bio], Biophysics, In Vitro Techniques, 01 natural sciences, Signal, 030218 nuclear medicine & medical imaging, 03 medical and health sciences, 0302 clinical medicine, 0103 physical sciences, Animals, Radiology, Nuclear Medicine and imaging, 010301 acoustics, Radiological and Ultrasound Technology, business.industry, Attenuation, Detector, Ultrasound, Uncertainty, [SDV] Life Sciences [q-bio], Amplitude, Blood-Brain Barrier, Cavitation, Microbubbles, Blood-brain barrier disruption, business, Biomedical engineering
Abstract: Exposure to ultrasound combined with intravenous injection of microbubbles is a technique that can be used to temporarily disrupt the blood-brain barrier. Transcranial monitoring of cavitation can be done with one or more passive cavitation detectors (PCDs). However, the positioning of the PCDs relative to the cavitation site and the attenuation of these signals by the skull are two sources of error in the quantification of cavitation activity. The aim of this study was to evaluate in vitro the amplitude variation of cavitation signals that can be expected for an excised porcine skull model. The variation caused by the relative positioning of the PCD with respect to the cavitation site was quantified. A position-based correction of the signal amplitude was evaluated. Pig skull samples were used to assess variation in signal amplitude caused by bone. The overall coefficient of variation of the signals owing to these measurement biases was estimated at 30.8%.
Published: 2020
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24. Measles Virus-Imposed Remodeling of the Autophagy Machinery Determines the Outcome of Bacterial Coinfection

Author: Mathieu Claviere, Aude Lavedrine, Guénaëlle Lamiral, Mariette Bonnet, Pauline Verlhac, Denitsa S. Petkova, Lucile Espert, Rémi Duclaux-Loras, Julie Lucifora, Michel Rivoire, Gilles Boschetti, Stéphane Nancey, Aurore Rozières, Christophe Viret, Mathias Faure, Centre International de Recherche en Infectiologie (CIRI), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut de Recherche en Infectiologie de Montpellier (IRIM), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM), Hospices Civils de Lyon (HCL), Application des ultrasons à la thérapie (LabTAU), Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre International de Recherche en Infectiologie - UMR (CIRI), École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Viret, Christophe, Oncogenèse rétrovirale – Retroviral Oncogenesis (OR), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Autophagie infection et immunité - Autophagy Infection Immunity (APY), Département cancer environnement (Centre Léon Bérard - Lyon), Centre Léon Bérard [Lyon], and Centre Hospitalier Lyon Sud [CHU - HCL] (CHLS)
Subjects: co-infection, [SDV]Life Sciences [q-bio], [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], Autophagy, Cell Biology, virus, [SDV.BC.IC] Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], bacteria, syncytia, Molecular Biology
Abstract: Although it is admitted that secondary infection can complicate viral diseases, the consequences of viral infection on cell susceptibility to other infections remain underexplored at the cellular level. We though to examine whether the sustained macroautophagy/autophagy associated with measles virus (MeV) infection could help cells oppose invasion by Salmonella Typhimurium, a bacterium sensitive to autophagic restriction. We report here the unexpected finding that Salmonella markedly replicated in MeV-infected cultures due to selective growth within multinucleated cells. Hyper-replicating Salmonella localized outside of LAMP1-positive compartments to an extent that equaled that of the predominantly cytosolic sifA mutant Salmonella. Bacteria were subjected to effective ubiquitination but failed to be targeted by LC3 despite an ongoing productive autophagy. Such a phenotype could not be further aggravated upon silencing of the selective autophagy regulator TBK1 or core autophagy factors ATG5 or ATG7. MeV infection also conditioned primary human epithelial cells for augmented Salmonella replication. The analysis of selective autophagy receptors able to target Salmonella revealed that a lowered expression level of SQSTM1/p62 and TAX1BP1/T6BP autophagy receptors prevented effective anti-Salmonella autophagy in MeV-induced syncytia. Conversely, as SQSTM1/p62 is promoting the cytosolic growth of Shigella flexneri, MeV infection led to reduced Shigella replication. The results indicate that the rarefaction of dedicated autophagy receptors associated with MeV infection differentially affects the outcome of bacterial coinfection depending on the nature of the functional relationship between bacteria and such receptors. Thus, virus-imposed reconfiguration of the autophagy machinery can be instrumental in determining the fate of bacterial coinfection.Abbreviations: ACTB/β-ACTIN: actin beta; ATG: autophagy related; BAFA1: bafilomycin A1; CFU: colony-forming units; CALCOCO2/NDP52: calcium binding and coiled-coil domain 2; FIP: fusion inhibitory peptide; GFP: green fluorescent protein; LAMP1: lysosomal associated membrane protein 1; LIR: MAP1LC3/LC3-interacting region; MAP1LC3/LC3: microtubule associated protein 1 light chain 3; MeV: measles virus; MOI: multiplicity of infection; OPTN: optineurin; PHH: primary human hepatocyte; SCV: Salmonella-containing vacuoles; SQSTM1/p62: sequestosome 1; S. flexneri: Shigella flexneri; S. Typhimurium: Salmonella enterica serovar Typhimurium; TAX1BP1/T6BP: Tax1 binding protein 1; TBK1: TANK binding kinase 1
Published: 2022
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25. Endoscopic Submucosal Dissection of High-Grade Dysplasia Recurrence in Ulcerative Colitis Using a Multitraction Technique

Author: Thomas Lambin, Clara Yzet, Joanna Pofelski, Eddy Cotte, Mathurin Fumery, Pierre Lafeuille, Mathieu Pioche, Service d'Hépato Gastroenterologie [CHU Amiens-Picardie], CHU Amiens-Picardie, Hospices Civils de Lyon (HCL), Institut Pascal (IP), Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne (UCA)-Institut national polytechnique Clermont Auvergne (INP Clermont Auvergne), Université Clermont Auvergne (UCA)-Université Clermont Auvergne (UCA), Périnatalité et Risques Toxiques - UMR INERIS_I 1 (PERITOX), Institut National de l'Environnement Industriel et des Risques (INERIS)-Université de Picardie Jules Verne (UPJV)-CHU Amiens-Picardie, Département de Gastroentérologie et hépatologie, Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble-Hôpital Michallon, Centre Hospitalier Lyon Sud [CHU - HCL] (CHLS), Université de Lyon, Laboratoire de Biométrie et Biologie Evolutive - UMR 5558 (LBBE), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS), Application des ultrasons à la thérapie (LabTAU), Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM), and Société Française d'Endoscopie Digestive
Subjects: medicine.medical_specialty, Endoscopic Mucosal Resection/methods, Hyperplasia, Endoscopic Mucosal Resection, Colonoscopy/methods, business.industry, High grade dysplasia, Colitis, Gastroenterology, Endoscopic submucosal dissection, Colonoscopy, medicine.disease, Ulcerative colitis, Treatment Outcome, Hyperplasia/pathology, Ulcerative/pathology/surgery, Medicine, Humans, Colitis, Ulcerative, Radiology, Intestinal Mucosa, business, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology, Intestinal Mucosa/diagnostic imaging/pathology/surgery
Abstract: International audience
Published: 2022
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26. Ultrasound to CT rigid image registration using CNN for the HIFU treatment of heart arrhythmias

Author: Batoul Dahman, francis bessier, Jean-Louis Dillenseger, Dillenseger, Jean-Louis, Développement d'une sonde HIFU trans oesophagienne pour le traitement des arythmies cardiaques - - CHORUS2017 - ANR-17-CE19-0017 - AAPG2017 - VALID, Laboratoire Traitement du Signal et de l'Image (LTSI), Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM), Application des ultrasons à la thérapie (LabTAU), Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM), and ANR-17-CE19-0017,CHORUS,Développement d'une sonde HIFU trans oesophagienne pour le traitement des arythmies cardiaques(2017)
Subjects: [SDV.IB] Life Sciences [q-bio]/Bioengineering, Multimodal image fusion, Ultrasound Imaging-cardiac, [INFO.INFO-IM] Computer Science [cs]/Medical Imaging, [INFO.INFO-IM]Computer Science [cs]/Medical Imaging, [SDV.IB]Life Sciences [q-bio]/Bioengineering, Rigid-body image registration, [SPI.SIGNAL]Engineering Sciences [physics]/Signal and Image processing, [SPI.SIGNAL] Engineering Sciences [physics]/Signal and Image processing
Abstract: International audience; Image-guided thermal ablations have become an important therapeutic option for patient with cardiac arrhythmia, it is minimally-invasive and provides better and faster patient recovery. However, to enhance the ablation guidance, the therapist needs to link by image registration the intraoperative images to the high-resolution anatomical preoperative imaging, in which the ablation path has been defined. In this work, we present a convolutional neural networks (CNNs) framework for transesophageal ultrasound/computed tomography image registration to solve the problem of high computation time of the classical iterative methods, which is not suitable for a real-time application. We propose the following process: we first pass the input moving and fixed image pairs through a siamese architecture consisting of convolutional layers, thus extracting features of moving and fixed maps analogous to dense local descriptors, then matching the feature maps, and finally pass this correspondence feature map into a registration network, which directly outputs the registration parameters set of the rigid registration. Accuracy of the registration is quantified based on the Target Registration Error (TRE) for specific anatomical landmarks. Results of the registration process show a median TRE of 2.2 mm for all the fiducial points, and the registration computation time was around 3 ms comparing to the classic iterative methods which takes around 70 seconds for one image pair. In our future work we are going to perform our approach on 2D/3D learning-based registration to refine the estimation of the transesophageal probe pose in the 3D preoperative volume.
Published: 2022
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27. Ultrasound scattering from cell-pellet biophantoms and ex vivo tumors provides insight into the cellular structure involved in scattering

Author: Muleki Seya, Pauline, Muleki-Seya, Pauline, O'Brien, William, Laboratoire de Mécanique et d'Acoustique [Marseille] (LMA ), Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Centre National de la Recherche Scientifique (CNRS), Centre de Recherche en Acquisition et Traitement de l'Image pour la Santé (CREATIS), Université Jean Monnet [Saint-Étienne] (UJM)-Hospices Civils de Lyon (HCL)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Application des ultrasons à la thérapie (LabTAU), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre Léon Bérard [Lyon]-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Langevin - Ondes et Images (UMR7587) (IL), Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP), Bioacoustics Research Laboratory, Bioacoustics Research Laboratory, Dept Elect. and Comp. Engineering, Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Hospices Civils de Lyon (HCL)-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA), Imagerie Ultrasonore, Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Hospices Civils de Lyon (HCL)-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), University of Illinois at Urbana-Champaign [Urbana], University of Illinois System, and MULEKI SEYA, PAULINE
Subjects: Materials science, Acoustics and Ultrasonics, Quantitative Biology::Tissues and Organs, Physics::Medical Physics, Normal Distribution, Article, Quantitative Biology::Cell Behavior, symbols.namesake, Nuclear magnetic resonance, Neoplasms, medicine, Scattering parameters, Humans, Electrical and Electronic Engineering, Instrumentation, ComputingMilieux_MISCELLANEOUS, Ultrasonography, Scattering, Histological Techniques, Cellular Structures, [PHYS.MECA.ACOU]Physics [physics]/Mechanics [physics]/Acoustics [physics.class-ph], Cell Pellet, medicine.anatomical_structure, Volume fraction, symbols, [PHYS.MECA.ACOU] Physics [physics]/Mechanics [physics]/Acoustics [physics.class-ph], Structure factor, Nucleus, Gaussian network model, Ex vivo
Abstract: International audience; The histologically identifiable cellular structure(s) involved in ultrasonic scattering is(are) yet to be uniquely identified. The study quantifies six possible cellular scattering parameters, namely, cell and nucleus radii and their respective cell and nucleus volume fractions as well as a combination of cell and nucleus radii and their volume fraction. The six cellular parameters are each derived from four cell lines (4T1, JC, LMTK, and MAT) and two tissue types (cell-pellet biophantom and ex vivo tumor). Optical histology and quantitative ultrasound (QUS), both independent approaches, are used to yield these cellular parameters. QUS scatterer parameters are experimentally determined using two ultrasonic scattering models: the spherical Gaussian model (GM) and the structure factor model (SFM) to yield insight about scattering from nuclei only and cells only. GM is a classical ultrasonic scattering model to evaluate QUS parameters and is well adapted for diluted media. SFM is adapted for dense media to estimate reasonably well scatterer parameters of cellular structures from ex vivo tissue. Nucleus and cell radii and volume fractions are measured optically from histology. They were used as inputs to calculate BSC for scattering from cells, nuclei, and both cells and nuclei. The QUS-derived scatterers (radii and volume fractions) distributions were then compared to the optical histology scatterer parameters derived from these calculated BSCs. The results suggest scattering from cells only (LMTK and MAT) or both cells and nuclei (4T1 and JC) for cell-pellet biophantoms and scattering from nuclei only for tumors.
Published: 2021
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28. Development of a Numerical Model of High-Intensity Focused Ultrasound Treatment in Mobile and Elastic Organs: Application to a Beating Heart

Author: Elodie Cao, Paul Greillier, Raphaël Loyet, Françoise Chavrier, Jade Robert, Francis Bessière, Jean-Louis Dillenseger, Cyril Lafon, Centre Léon Bérard [Lyon], Application des ultrasons à la thérapie (LabTAU), Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM), Hospices Civils de Lyon (HCL), Laboratoire Traitement du Signal et de l'Image (LTSI), Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Rennes 1 (UR1), and Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: Cardiac applications, Mobile organs, Acoustics and Ultrasonics, Radiological and Ultrasound Technology, Bioheat transfer equation, Biophysics, Temperature, Finite-volume method, Sonication, High-intensity focused ultrasound, Curvilinear grid, cardiovascular system, High-Intensity Focused Ultrasound Ablation, Radiology, Nuclear Medicine and imaging, [SDV.IB]Life Sciences [q-bio]/Bioengineering, Computer Simulation
Abstract: International audience; High-intensity focused ultrasound (HIFU) is a promising method used to treat cardiac arrhythmias, as it can induce lesions at a distance throughout myocardium thickness. Numerical modeling is commonly used for ultrasound probe development and optimization of HIFU treatment strategies. This study was aimed at describing a numerical method to simulate HIFU thermal ablation in elastic and mobile heart models. The ultrasound pressure field is computed on a 3-D orthonormal grid using the Rayleigh integral method, and the attenuation is calculated step by step between cells. The temperature distribution is obtained by resolution of the bioheat transfer equation on a 3-D non-orthogonally structured curvilinear grid using the finite-volume method. The simulation method is applied on two regions of the heart (atrioventricular node and ventricular apex) to compare the thermal effects of HIFU ablation depending on deformation, motion type and amplitude. The atrioventricular node requires longer sonication than the ventricular apex to reach the same lesion volume. Motion considerably influences treatment duration, lesion shape and distribution in cardiac HIFU treatment. These results emphasize the importance of considering local motion and deformation in numerical studies to define efficient and accurate treatment strategies.
Published: 2021
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29. Effectiveness of Non-Pharmacological Interventions for Irritable Bowel Syndrome: A Systematic Review

Author: François Mion, Xavier Armoiry, Florent Amsallem, Stéphane Sanchez, Application des ultrasons à la thérapie (LabTAU), Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre Hospitalier de Troyes, Matériaux, ingénierie et science [Villeurbanne] (MATEIS), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS), Hôpital Edouard Herriot [CHU - HCL], Hospices Civils de Lyon (HCL), and University of Warwick [Coventry]
Subjects: medicine.medical_specialty, Abdominal pain, MEDLINE, Review Article, Cochrane Library, law.invention, [SPI.MAT]Engineering Sciences [physics]/Materials, 03 medical and health sciences, Other systems of medicine, 0302 clinical medicine, Randomized controlled trial, law, medicine, Acupuncture, 030212 general & internal medicine, Intensive care medicine, Irritable bowel syndrome, business.industry, medicine.disease, Osteopathic medicine in the United States, 3. Good health, Complementary and alternative medicine, Supportive psychotherapy, 030211 gastroenterology & hepatology, medicine.symptom, business, RZ201-999, RC
Abstract: International audience; Introduction: Given the complexity of the therapeutic management of irritable bowel syndrome (IBS), alternative non-pharmacological therapies are frequently offered to patients. The aim of this study was to conduct a systematic review in order to establish the current evidence base for non-pharmacological interventions (body-directed and mind-body therapies) in the management of IBS.Materials and methods: The literature was searched in several electronic databases (PubMed (including Medline), Web of Science (Clarivate Analytics), Scopus (Elsevier), ScienceDirect (Elsevier), Cochrane Library (Wiley), and Wiley Online Library (Wiley)) for randomized controlled trials (RCTs) published in the English language from 1990 to 2020. Effectiveness outcomes were examined through the change in overall IBS symptoms or abdominal pain up to 12 months after treatment.Results: 11 studies (parallel-group RCTs) were identified that enrolled 1590 participants in total. Body-directed therapies (acupuncture and osteopathic medicine) showed a beneficial effect compared with standard medical treatment for overall IBS symptoms at 6 months follow-up, while no study found any difference between body-directed and sham therapies for abdominal pain or overall IBS symptoms. It was not possible to conclude whether hypnotherapy was superior to standard medical treatment or supportive therapy for overall IBS symptoms or abdominal pain due to discordant results.Conclusions: Although body-directed therapies such as acupuncture and osteopathic medicine may be beneficial for overall IBS symptoms, higher-quality RCTs are needed to establish the clinical benefit of non-pharmacological interventions for IBS. An important challenge will be the definition of the optimal control groups to be used in non-pharmacological trials.
Published: 2021
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30. Indications of Lefort osteotomies for facial deformities induced by craniosynostosis

Author: Arnaud Gleizal, F. Di Rocco, J. Chauvel-Picard, Application des ultrasons à la thérapie (LabTAU), Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Centre Léon Bérard [Lyon]-Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: Adolescent, [SDV]Life Sciences [q-bio], medicine.medical_treatment, Treatment outcome, Osteotomy, Craniosynostosis, Craniosynostoses, 03 medical and health sciences, 0302 clinical medicine, medicine, Humans, Osteotomy, Le Fort, Child, Orthodontics, business.industry, Craniofacial Dysostosis, medicine.disease, 3. Good health, Treatment Outcome, Face, 030220 oncology & carcinogenesis, Female, Surgery, Neurology (clinical), business, 030217 neurology & neurosurgery
Abstract: Neurochirurgie - Sous presse. Epreuves corrigees par l'auteur. Disponible en ligne depuis le dimanche 20 octobre 2019
Published: 2019
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31. A causal study of the phenomenon of ultrasound neurostimulation applied to an in vivo invertebrate nervous model

Author: Jean-Louis Mestas, W. Apoutou N'Djin, Alexandre Carpentier, Jérémy Vion-Bailly, Ivan Suarez Castellanos, Jean-Yves Chapelon, Centre Léon Bérard [Lyon], Application des ultrasons à la thérapie (LabTAU), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre Léon Bérard [Lyon]-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Lyon, Service de Neurochirurgie [CHU Pitié-Salpêtrière], CHU Pitié-Salpêtrière [AP-HP], and Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)
Subjects: medicine.medical_treatment, lcsh:Medicine, Stimulation, Ultrasound stimulation, Neural circuits, Article, Afferent Neurons, Focused ultrasound, 030218 nuclear medicine & medical imaging, 03 medical and health sciences, 0302 clinical medicine, In vivo, medicine, Animals, Neurons, Afferent, Oligochaeta, lcsh:Science, Neurostimulation, Ultrasonography, Multidisciplinary, business.industry, Ultrasound, lcsh:R, Acoustics, Invertebrates, Axons, Electric Stimulation, Ultrasonic Waves, Early results, Models, Animal, [PHYS.PHYS.PHYS-MED-PH]Physics [physics]/Physics [physics]/Medical Physics [physics.med-ph], [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], lcsh:Q, business, Biomedical engineering, Neuroscience, 030217 neurology & neurosurgery
Abstract: Focused ultrasound are considered to be a promising tool for the treatment of neurological conditions, overcoming the limitations of current neurostimulation techniques in terms of spatial resolution and invasiveness. Much evidence to support the feasibility of ultrasound activation of neurons at the systemic level has already been provided, but to this day, the biophysical mechanisms underlying ultrasound neurostimulation are still widely unknown. In order to be able to establish a clear and robust causality between acoustic parameters of the excitation and neurobiological characteristics of the response, it is necessary to work at the cellular level, or alternatively on very simple animal models. The study reported here responds to three objectives. Firstly, to propose a simple nervous model for the study of the ultrasound neurostimulation phenomenon, associated with a clear and simple experimental protocol. Secondly, to compare the characteristics of this model’s nervous response to ultrasound neurostimulation with its nervous response to mechanical and electrical stimulation. Thirdly, to study the role played by certain acoustic parameters in the success rate of the phenomenon of ultrasound stimulation. The feasibility of generating action potentials (APs) in the giant axons of an earthworm’s ventral nerve cord, using pulsed ultrasound stimuli (f = 1.1 MHz, Ncycles = 175–1150, PRF = 25–125 Hz, Npulses = 20, PA = 2.5–7.3 MPa), was demonstrated. The time of generation (TOG) of APs associated with ultrasound stimulation was found to be significantly shorter and more stable than the TOG associated with mechanical stimulation (p p p
Published: 2019
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32. High-Intensity Focused Ultrasound Using a Toroidal Transducer as an Adjuvant Treatment for Placenta Accreta: A Preliminary Ex Vivo Study

Author: S. Cambronero, J. Caloone, David Melodelima, C. Huissoud, M. Sanchez, Victor Barrere, Application des ultrasons à la thérapie (LabTAU), Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut cellule souche et cerveau (U846 Inserm - UCBL1), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique (INRA), Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon
Subjects: Placenta accreta, [SDV]Life Sciences [q-bio], medicine.medical_treatment, 0206 medical engineering, Biomedical Engineering, Biophysics, 02 engineering and technology, 030218 nuclear medicine & medical imaging, Gross examination, Lesion, 03 medical and health sciences, 0302 clinical medicine, Placenta, medicine, business.industry, Ultrasound, Myometrium, medicine.disease, 020601 biomedical engineering, High-intensity focused ultrasound, 3. Good health, medicine.anatomical_structure, medicine.symptom, business, Nuclear medicine, Ex vivo
Abstract: Background Placenta accreta is an abnormal invasion of the placenta within the myometrium, which has increasing rate and could result in maternal mortality or morbidity. The objective of this study was to evaluate the feasibility of treating placenta accreta with high-intensity focused ultrasound (HIFU) using an ex vivo model. Material and methods A toroidal HIFU transducer working at 2.5 MHz with an integrated ultrasound-imaging probe was used. Ex vivo experiments were performed in eight human placentae (from 39 to 40 weeks) within a configuration that mimicked clinical conditions as closely as possible. The fetal side of the placenta was placed in contact with the sterile envelope that covered the HIFU device to simulate the uterine serosa. HIFU lesions were studied on sonograms and macroscopically. Results In total, 19 single HIFU lesions were created with the same exposure parameters for all of the lesions. The exposure time was 75 s. The acoustic power was 90 W. HIFU lesions were created at different distances (2, 6, 7 and 8 mm) from the uterine serosa by adjusting the thickness of the coupling water and thus, the distance between the HIFU emitters and placental tissues. All of the lesions were homogeneous and approximately spherical in shape. The two diameters of the HIFU lesions were on average 14.6 ± 2.1 mm and 14.1 ± 2.3 mm . Lesions were immediately visible on sonograms after each HIFU exposure as a hyperechoic region. The distance between the surface of the placenta and HIFU lesion as measured on ultrasound images was closely correlated with the same distance that was measured on gross pathology ( r = 0.89 , p 0.05 ). Conclusions This study demonstrates that HIFU may be an interesting option for adjuvant treatment during a cesarean of placenta accreta. These results must be validated under real clinical conditions.
Published: 2019
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33. Ultrasound-Targeted Microbubble Destruction (UTMD) for Localized Drug Delivery into Tumor Tissue

Author: F. Padilla, Jennifer Wischhusen, Application des ultrasons à la thérapie (LabTAU), Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre de Recherche en Cancérologie de Lyon (UNICANCER/CRCL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'Imagerie Paramétrique (LIP), and Université Pierre et Marie Curie - Paris 6 (UPMC)-IFR58-Centre National de la Recherche Scientifique (CNRS)
Subjects: business.industry, [SDV]Life Sciences [q-bio], 0206 medical engineering, Ultrasound, Biomedical Engineering, Biophysics, 02 engineering and technology, 020601 biomedical engineering, Tumor tissue, 030218 nuclear medicine & medical imaging, 3. Good health, 03 medical and health sciences, 0302 clinical medicine, In vivo, Cavitation, Drug delivery, Microbubbles, Medicine, Nanomedicine, business, Drug carrier, Biomedical engineering
Abstract: Background Ultrasound-targeted microbubble destruction (UTMD) is a type of ultrasound therapy, in which low frequency moderate power ultrasound is combined with microbubbles to trigger cavitation. Cavitation is the process of oscillation of gas bubbles causing biophysical effects such as pushing and pulling or shock waves that permeabilize biological barriers. In vivo, cavitation results in tissue permeabilization and is used to enable local delivery of nanomedicine. While cavitation can occur in biological liquids when high pressure ultrasound is applied, the use of microbubbles as cavitation nuclei in UTMD largely facilitates the induction of cavitation. UTMD is intensively studied for drug delivery into tumor tissue, but also for the activation of anti-tumor immune responses. The first clinical studies of UTMD-mediated chemotherapy delivery confirmed safety and efficacy of this approach. Aim The present review summarizes ultrasound settings, cavitation approaches, biophysical mechanisms of drug delivery, drug carriers, and pre-clinical and clinical applications of UTMD for drug delivery into tumors.
Published: 2019
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34. Acoustic Su-Schrieffer-Heeger lattice: Direct mapping of acoustic waveguides to the Su-Schrieffer-Heeger model

Author: Antonin Coutant, Georgios Theocharis, Vincent Pagneux, Li-Yang Zheng, Vassos Achilleos, Olivier Richoux, Audrey Sivadon, Laboratoire d'Acoustique de l'Université du Mans (LAUM), Le Mans Université (UM)-Centre National de la Recherche Scientifique (CNRS), Institut de Mathématiques de Bourgogne [Dijon] (IMB), Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université de Bourgogne (UB), Application des ultrasons à la thérapie (LabTAU), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre Léon Bérard [Lyon]-Institut National de la Santé et de la Recherche Médicale (INSERM), Universidad Carlos III de Madrid [Madrid] (UC3M), European Commission843152Region Pays de la LoireNoHENA project under the program Etoiles Montantes of the Region Pays de la Loire, Centre National de la Recherche Scientifique (CNRS)-Le Mans Université (UM), Université de Bourgogne (UB)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre National de la Recherche Scientifique (CNRS), Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM), and université de Bourgogne, IMB
Subjects: FOS: Physical sciences, 02 engineering and technology, Physics - Classical Physics, Edge (geometry), [SPI.MAT] Engineering Sciences [physics]/Materials, 01 natural sciences, [PHYS] Physics [physics], [SPI.MAT]Engineering Sciences [physics]/Materials, [SPI]Engineering Sciences [physics], Simple (abstract algebra), Robustness (computer science), 0103 physical sciences, Mesoscale and Nanoscale Physics (cond-mat.mes-hall), Boundary value problem, 010306 general physics, Electronic band structure, Physics, [PHYS]Physics [physics], Condensed Matter - Mesoscale and Nanoscale Physics, Classical Physics (physics.class-ph), 021001 nanoscience & nanotechnology, Wave equation, states, Lattice (module), Classical mechanics, Homogeneous space, 0210 nano-technology
Abstract: Topological physics strongly relies on prototypical lattice model with particular symmetries. We report here on a theoretical and experimental work on acoustic waveguides that is directly mapped to the one-dimensional Su-Schrieffer-Heeger chiral model. Starting from the continuous two dimensional wave equation we use a combination of monomadal approximation and the condition of equal length tube segments to arrive at the wanted discrete equations. It is shown that open or closed boundary conditions topological leads automatically to the existence of edge modes. We illustrate by graphical construction how the edge modes appear naturally owing to a quarter-wavelength condition and the conservation of flux. Furthermore, the transparent chirality of our system, which is ensured by the geometrical constraints allows us to study chiral disorder numerically and experimentally. Our experimental results in the audible regime demonstrate the predicted robustness of the topological edge modes., 10 pages, 11 figures. Slight modification of title, minor additions to the text
Published: 2021
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35. Low FODMAPs diet or usual dietary advice for the treatment of refractory gastroesophageal reflux disease: An open‐labeled randomized trial

Author: Emiliane Rolland, S Sacher-Huvelin, François Mion, Stanislas Bruley des Varannes, Pauline Rivière, Chloé Melchior, Guillaume Gourcerol, Frank Zerbib, Sabine Roman, Blandine Vauquelin, Hôpital Haut-Lévêque [CHU Bordeaux], CHU Bordeaux [Bordeaux], CIC Bordeaux, Université Bordeaux Segalen - Bordeaux 2-Institut National de la Santé et de la Recherche Médicale (INSERM), Nutrition, inflammation et dysfonctionnement de l'axe intestin-cerveau (ADEN), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institute for Research and Innovation in Biomedicine (IRIB), Normandie Université (NU)-Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Service d'Hépato-Gastroentérologie [CHU Rouen], Hôpital Charles Nicolle [Rouen]-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-CHU Rouen, Normandie Université (NU), Centre d'Investigation Clinique [CHU Rouen] (CIC Rouen), Hôpital Charles Nicolle [Rouen]-CHU Rouen, Normandie Université (NU)-Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM), UNIROUEN - UFR Santé (UNIROUEN UFR Santé), Normandie Université (NU)-Normandie Université (NU), Hôpital Edouard Herriot [CHU - HCL], Hospices Civils de Lyon (HCL), Application des ultrasons à la thérapie (LabTAU), Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre hospitalier universitaire de Nantes (CHU Nantes), Centre d’Investigation Clinique de Nantes (CIC Nantes), Université de Nantes (UN)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre hospitalier universitaire de Nantes (CHU Nantes), Service de physiologie digestive, urinaire, respiratoire et de l'exercice [CHU Rouen], CHU Rouen, and Normandie Université (NU)-Hôpital Charles Nicolle [Rouen]
Subjects: Adult, Male, medicine.medical_specialty, Physiology, medicine.drug_class, Proton-pump inhibitor, Disease, Gastroenterology, law.invention, Diet, Carbohydrate-Restricted, 03 medical and health sciences, 0302 clinical medicine, Randomized controlled trial, Refractory, law, Internal medicine, medicine, Clinical endpoint, Humans, ComputingMilieux_MISCELLANEOUS, Endocrine and Autonomic Systems, business.industry, Reflux, [SDV.MHEP.HEG]Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology, Middle Aged, medicine.disease, 3. Good health, Clinical trial, 030220 oncology & carcinogenesis, Gastroesophageal Reflux, GERD, Female, 030211 gastroenterology & hepatology, Fermented Foods, business
Abstract: BACKGROUND The low FODMAPs (fermentable oligo-, di-, monosaccharides, and polyols) diet improves lower gastrointestinal symptoms. Patients suffering from proton pump inhibitor (PPI) refractory gastroesophageal reflux disease (GERD) have limited treatment options. We investigated the efficacy of a low FODMAPs diet in patients with PPI refractory GERD. METHODS This multicenter, randomized, open-label study compared the efficacy of a 4-week low FODMAPs diet and usual dietary advice (ie, low-fat diet and head of bed elevation) in patients with symptomatic PPI refractory GERD, defined by a Reflux Disease Questionnaire (RDQ) score >3 and abnormal pH-impedance monitoring on PPIs. The primary endpoint was the percentage of responders (RDQ ≤3) at the end of the diet. RESULTS Thirty-one patients (55% women, median age 45 years) were included, 16 randomized in the low FODMAPs diet group and 15 in the usual dietary advice group. Adherence to the assigned diet was good, with a significant difference in the FODMAPs intake per day between the low FODMAPs diet (2.5 g) and the usual dietary advice group (13 g) (p
Published: 2021
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36. Experimental observation of micrometric bubble pair oscillations and induced effects

Author: Regnault, Gabriel, Mauger, Cyril, Doinikov, A. A., Blanc-Benon, Philippe, Inserra, Claude, Laboratoire de Mecanique des Fluides et d'Acoustique (LMFA), École Centrale de Lyon (ECL), Université de Lyon-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS), Application des ultrasons à la thérapie (LabTAU), Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM), and Mauger, Cyril
Subjects: Physics::Fluid Dynamics, [SPI]Engineering Sciences [physics], [SPI] Engineering Sciences [physics], microstreaming, shape modes, Bubble pair oscillations
Abstract: International audience; The study of an acoustic bubble pair is a first step in the understanding of mechanisms ruling cavitation clouds and groups of contrast agents. Such bubbles interact through acoustic coupling but can also be influenced by the fluid flows that they generate. Here, this fluid interaction is evidenced for a micro-metric bubble pair. Conditions on shape instabilities needed to obtain such interactions are shown using experimental observations of free bubble induced fluid flows.
Published: 2021

37. Endoscopic characterization of colorectal neoplasia with different published classifications: comparative study involving CONECCT classification

Author: Bonniaud, Paul, Jacques, Jérémie, Lambin, Thomas, Gonzalez, Jean-Michel, Dray, Xavier, Coron, Emmanuel, Leblanc, Sarah, Chevaux, Jean-Baptiste, Léger-Nguyen, Florence, Hamel, Benjamin, Lienhart, Isabelle, Rivory, Jérôme, Ponchon, Thierry, Saurin, Jean-Christophe, Monzy, Frédéric, Legros, Romain, Lépilliez, Vincent, Subtil, Fabien, Barret, Maximilien, Pioche, Mathieu, HAL-SU, Gestionnaire, Hospices Civils de Lyon (HCL), Hôpital Dupuytren [CHU Limoges], Société Française d'Endoscopie Digestive, Service de chirurgie générale et digestive [CHU Saint-Antoine], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Saint-Antoine [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU), Centre hospitalier universitaire de Nantes (CHU Nantes), Clinique Jean-Mermoz, Hôpital privé Jean Mermoz, Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy), Clinique du Val d'Ouest, Hôpital Villefranche sur Saône, Centre Hospitalier d'Annecy, Centre hospitalier d'Annecy, Hôpital-Clinique Claude-Bernard [Metz], Hôpital Cochin [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Laboratoire de Biométrie et Biologie Evolutive - UMR 5558 (LBBE), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS), Application des ultrasons à la thérapie (LabTAU), Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire de Biomécanique Appliquée (LBA UMR T24), Aix Marseille Université (AMU)-Université Gustave Eiffel, CHU Saint-Antoine [AP-HP], and Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)
Subjects: Original article, [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, Pharmacology (medical), RC799-869, Diseases of the digestive system. Gastroenterology, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract: Background and study aims The aim of this study was to validate the COlorectal NEoplasia Classification to Choose the Treatment (CONECCT) classification that groups all published criteria (including covert signs of carcinoma) in a single table. Patients and methods For this multicenter comparative study an expert endoscopist created an image library (n = 206 lesions; from hyperplastic to deep invasive cancers) with at least white light Imaging and chromoendoscopy images (virtual ± dye based). Lesions were resected/biopsied to assess histology. Participants characterized lesions using the Paris, Laterally Spreading Tumours, Kudo, Sano, NBI International Colorectal Endoscopic Classification (NICE), Workgroup serrAted polypS and Polyposis (WASP), and CONECCT classifications, and assessed the quality of images on a web-based platform. Krippendorff alpha and Cohen’s Kappa were used to assess interobserver and intra-observer agreement, respectively. Answers were cross-referenced with histology. Results Eleven experts, 19 non-experts, and 10 gastroenterology fellows participated. The CONECCT classification had a higher interobserver agreement (Krippendorff alpha = 0.738) than for all the other classifications and increased with expertise and with quality of pictures. CONECCT classification had a higher intra-observer agreement than all other existing classifications except WASP (only describing Sessile Serrated Adenoma Polyp). Specificity of CONECCT IIA (89.2, 95 % CI [80.4;94.9]) to diagnose adenomas was higher than the NICE2 category (71.1, 95 % CI [60.1;80.5]). The sensitivity of Kudo Vi, Sano IIIa, NICE 2 and CONECCT IIC to detect adenocarcinoma were statistically different (P Conclusions The CONECCT classification currently offers the best interobserver and intra-observer agreement, including between experts and non-experts. CONECCT IIA is the best classification for excluding presence of adenocarcinoma in a colorectal lesion and CONECCT IIC offers the better compromise for diagnosing superficial adenocarcinoma.
Published: 2021
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38. Spatio-Temporal Characterization Of Causal Electrophysiological Activity Stimulated By Single Pulse Focused Ultrasound: An Ex Vivo Study On Hippocampal Brain Slices

Author: Lea Salette, Gilles Huberfeld, Alexandre Carpentier, William Apoutou N'Djin, Elena Dossi, Jean-Yves Chapelon, Ivan Suarez-Castellanos, Jérémy Vion-Bailly, Application des ultrasons à la thérapie (LabTAU), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre Léon Bérard [Lyon]-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre interdisciplinaire de recherche en biologie (CIRB), Labex MemoLife, École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Collège de France (CdF (institution))-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Collège de France (CdF (institution))-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Service de Neurochirurgie [CHU Pitié-Salpêtrière], CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Centre d'investigation clinique Neurosciences [CHU Pitié Salpêtrière] (CIC Neurosciences), Suarez Castellanos, Ivan, Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM), École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Collège de France (CdF (institution))-Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Centre d'investigation clinique pluridisciplinaire [CHU Pitié Salpêtrière] (CIC-P 1421), Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], and Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP]
Subjects: Local Field Potential (LFP), medicine.medical_treatment, 0206 medical engineering, Biomedical Engineering, FOS: Physical sciences, Hippocampus, Stimulation, 02 engineering and technology, Local field potential, Physics - Classical Physics, Hippocampal formation, Biology, 03 medical and health sciences, Cellular and Molecular Neuroscience, 0302 clinical medicine, medicine, Single Pulse, Premovement neuronal activity, Focused Ultrasound (FUS), Neurostimulation, Ex-Vivo Mouse Hippocampus, MicroElectrode Arrays (MEA), [SDV.IB] Life Sciences [q-bio]/Bioengineering, [SCCO.NEUR]Cognitive science/Neuroscience, [SCCO.NEUR] Cognitive science/Neuroscience, Classical Physics (physics.class-ph), 020601 biomedical engineering, [PHYS.MECA.ACOU]Physics [physics]/Mechanics [physics]/Acoustics [physics.class-ph], Electrophysiology, medicine.anatomical_structure, [SDV.IB]Life Sciences [q-bio]/Bioengineering, Neuron, [PHYS.MECA.ACOU] Physics [physics]/Mechanics [physics]/Acoustics [physics.class-ph], Neuroscience, 030217 neurology & neurosurgery
Abstract: Objective. The brain operates via generation, transmission and integration of neuronal signals and most neurological disorders are related to perturbation of these processes. Neurostimulation by focused ultrasound (FUS) is a promising technology with potential to rival other clinically used techniques for the investigation of brain function and treatment of numerous neurological diseases. The purpose of this study was to characterize spatial and temporal aspects of causal electrophysiological signals directly stimulated by short, single pulses of FUS on ex vivo mouse hippocampal brain slices. Approach. Microelectrode arrays (MEAs) are used to study the spatio-temporal dynamics of extracellular neuronal activities both at the single neuron and neural networks scales. Hence, MEAs provide an excellent platform for characterization of electrical activity generated, modulated and transmitted in response to FUS exposure. In this study, a novel mixed FUS/MEA platform was designed for the spatio-temporal description of the causal responses generated by single 1.78 MHz FUS pulses in ex vivo mouse hippocampal brain slices. Main results. Our results show that FUS pulses can generate local field potentials (LFPs), sustained by synchronized neuronal post-synaptic potentials, and reproducing network activities. LFPs induced by FUS stimulation were found to be repeatable to consecutive FUS pulses though exhibiting a wide range of amplitudes (50–600 μV), durations (20–200 ms), and response delays (10–60 ms). Moreover, LFPs were spread across the hippocampal slice following single FUS pulses thus demonstrating that FUS may be capable of stimulating different neural structures within the hippocampus. Significance. Current knowledge on neurostimulation by ultrasound describes neuronal activity generated by trains of repetitive ultrasound pulses. This novel study details the causal neural responses produced by single-pulse FUS neurostimulation while illustrating the distribution and propagation properties of this neural activity along major neural pathways of the hippocampus.
Published: 2021
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39. Digital Holography-based Quantitative Elastography using Noise-Correlation

Author: Agathe Marmin, Simon Chatelin, Manuel Flury, Sybille Facca, Stefan Catheline, Amir Nahas, univOAK, Archive ouverte, Laboratoire des sciences de l'ingénieur, de l'informatique et de l'imagerie (ICube), Institut National des Sciences Appliquées - Strasbourg (INSA Strasbourg), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-École Nationale du Génie de l'Eau et de l'Environnement de Strasbourg (ENGEES)-Réseau nanophotonique et optique, Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Matériaux et nanosciences d'Alsace (FMNGE), Institut de Chimie du CNRS (INC)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Hopitaux Universitaires de Strasbourg - Département de Chirurgie de la main (SOS main - HUS), Application des ultrasons à la thérapie (LabTAU), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre Léon Bérard [Lyon]-Institut National de la Santé et de la Recherche Médicale (INSERM), École Nationale du Génie de l'Eau et de l'Environnement de Strasbourg (ENGEES)-Université de Strasbourg (UNISTRA)-Institut National des Sciences Appliquées - Strasbourg (INSA Strasbourg), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de Recherche en Informatique et en Automatique (Inria)-Les Hôpitaux Universitaires de Strasbourg (HUS)-Centre National de la Recherche Scientifique (CNRS)-Matériaux et Nanosciences Grand-Est (MNGE), Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Réseau nanophotonique et optique, Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: [SPI.AUTO] Engineering Sciences [physics]/Automatic, Physics::Medical Physics, [PHYS.MECA]Physics [physics]/Mechanics [physics], [PHYS.MECA] Physics [physics]/Mechanics [physics], [PHYS.MECA.BIOM]Physics [physics]/Mechanics [physics]/Biomechanics [physics.med-ph], [SPI.AUTO]Engineering Sciences [physics]/Automatic
Abstract: International audience; Quantitative elastography is performed using noise-correlation on full-field images acquired using digital holography. Experimental results isotropic and anisotropic polymer samples are presented as well as stiffness images on biological tissues.
Published: 2021

40. Assessment of irradiated socket healing in the rabbit's mandible: Experimental study

Author: Desoutter Aline, Béra Jean-Christophe, Barrot Laura, Chaux Anne-Gaëlle, Langonnet Stephan, Application des ultrasons à la thérapie (LabTAU), Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre Léon Bérard [Lyon], Epidémiologie des conduites addictives, Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon, and CCSD, Accord Elsevier
Subjects: Microscanner (microCT), medicine.medical_treatment, [SDV]Life Sciences [q-bio], Pilot Projects, Mandible, Bone healing, External Radiation Therapy, 03 medical and health sciences, 0302 clinical medicine, Bone Density, Region of interest, medicine, Animals, Bone mineral, Wound Healing, Radiotherapy, General Veterinary, business.industry, External radiotherapy, Irradiated bone, Rabbit (nuclear engineering), X-Ray Microtomography, 030206 dentistry, 3. Good health, [SDV] Life Sciences [q-bio], Radiation therapy, 030220 oncology & carcinogenesis, Female, Rabbits, Bone Diseases, Nuclear medicine, business
Abstract: International audience; As bone healing is altered after external radiation therapy, its evaluation is mandatory and lacks in clinical practice. The aim of the pilot study was to validate micro computed tomography (microCT) as a simple and reliable technique for assessing irradiated bone healing in the rabbit's mandible and compare with histologic findings. Nineteen rabbits (female New Zealand white) were used. The radiation protocol consisted of 5 sessions delivering 8.5 Gy each. MicroCT was performed at D0, D7, D14, D28 and D42 for the control group and D0, D28 and D42 for the irradiated group. A modified Perry's score was determined on histologic samples, and comparison between microCT and histological findings was performed. The main differences between irradiated and non-irradiated rabbits were observed at Day 28 and 42. There was a strong correlation between imaging and histologic findings. Radiation decreases bone quality and bone mineral density. As the correlation was strong between microCT and histologic findings, micro imaging could be considered as a simple and reliable technique to assess bone healing after radiation therapy and allows an easy comparison between samples, without invasive procedures. Great attention should be kept on the parameters and on the region of interest. The development of in-vivo microCT enlarges the perspectives of microCT use in experimental studies, avoiding invasive procedures, and preserving animal lives and well-being, and furthermore lead to clinical applications.
Published: 2020
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41. Pisarenko Class Beamformer Applied to Passive Acoustic Mapping of Ultrasound Cavitation

Author: Sivadon, Audrey, Polichetti, Maxime, Béra, Jean-Christophe, Varray, François, Nicolas, Barbara, Gilles, Bruno, Application des ultrasons à la thérapie (LabTAU), Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM), Imagerie Ultrasonore, Centre de Recherche en Acquisition et Traitement de l'Image pour la Santé (CREATIS), Université Jean Monnet [Saint-Étienne] (UJM)-Hospices Civils de Lyon (HCL)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Jean Monnet [Saint-Étienne] (UJM)-Hospices Civils de Lyon (HCL)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Inserra, Claude, Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Hospices Civils de Lyon (HCL)-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Hospices Civils de Lyon (HCL)-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Adaptive Beamforming, [PHYS.MECA.VIBR]Physics [physics]/Mechanics [physics]/Vibrations [physics.class-ph], [PHYS.MECA.VIBR] Physics [physics]/Mechanics [physics]/Vibrations [physics.class-ph], Therapeutic Ultrasound, [PHYS.MECA.ACOU] Physics [physics]/Mechanics [physics]/Acoustics [physics.class-ph], Passive Cavitation Imaging, [PHYS.MECA.ACOU]Physics [physics]/Mechanics [physics]/Acoustics [physics.class-ph]
Abstract: International audience; Cavitation induced by High Intensity Focused Ultrasound (HIFU) used in therapeutic ultrasound can be imaged passively using linear arrays and beamforming algorithms. A challenging purpose is to image both in spatial and temporal domain cavitation sources that are strongly involved in the tissue-ultrasound interaction, with a long-term objective of high image quality as well as real-time application. Recently, a formalism in the Fourier Domain using the Cross-Spectral Matrix (CSM), i.e. the spatial covariance matrix of the Fourier transform of received data, has been proposed to transpose adaptive algorithms currently used in array processing to this context. The strength of this formalism lies in combining the advantage of adaptive approaches in terms of image quality and enhancement of source localization, with a significant decrease of the numerical cost thanks to the use of Fourier Domain approaches. The present study focuses on an adaptive beamformer making use of the Pisarenko class methods, based on the p-th root compression of the CSM matrix. This method is compared to current state of the art in the context of passive cavitation imaging: The non-adaptive Frequency Domain Passive Cavitation Imaging (FD-PCI) and the adaptive Time Domain Robust Capon Beamformer (TD-RCB). The influence of the parameter p has been investigated on the resolution and the contrast of cavitation maps, and the parameter- free midway approach proposed by Stoica et al. is also considered. The performances of the method are illustrated using the simple case of single point-source simulations. The results show that it approaches the image quality of TD-RCB method, but with a computation time equivalent to FD-PCI. The Pisarenko class beamformer proves to be a promising tool combining good image quality characteristic of the adaptive approach with a significant reduction of the numerical computation time associated to the Fourier Domain implementation.
Published: 2020
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42. Simulation of High-Intensity Focused Ultrasound (HIFU) thermal ablation in Mobile and Elastic Organs

Author: Cao, Elodie, Greillier, Paul, Robert, Jade, Bessière, Francis, Loyet, Raphaël, Chavrier, Françoise, Dillenseger, Jean-Louis, Lafon, Cyril, Application des ultrasons à la thérapie (LabTAU), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre Léon Bérard [Lyon]-Institut National de la Santé et de la Recherche Médicale (INSERM), Hôpital Louis Pradel [CHU - HCL], Hospices Civils de Lyon (HCL), Laboratoire d'InfoRmatique en Image et Systèmes d'information (LIRIS), Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-École Centrale de Lyon (ECL), Université de Lyon-Université Lumière - Lyon 2 (UL2), Applications des ultrasons à la thérapie, Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire Traitement du Signal et de l'Image (LTSI), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 (UCBL), Université Lumière - Lyon 2 (UL2)-École Centrale de Lyon (ECL), Université de Lyon-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS), and Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: [PHYS.MECA.VIBR]Physics [physics]/Mechanics [physics]/Vibrations [physics.class-ph], Bio-Heat Transfer Equation, numerical simulation, High-Intensity Focused Ultrasound, cardiac motion, [PHYS.MECA.ACOU]Physics [physics]/Mechanics [physics]/Acoustics [physics.class-ph]
Abstract: International audience; Context: Numerical modeling is performed to optimize therapies that use HIFU. In cardiac procedures, heart movements and deformations make the treatment particularly challenging. This study aims to demonstrate with a new numerical model of thermal ablations that accurate modeling of temperature and thermal dose in elastic and mobile organs requires considering deformations and motions. Methods: Ultrasound pressure field is calculated using the Rayleigh integral method and a point-by-point estimation of the attenuation in the tissues. Temperature map is obtained by the discretization with finite volume method of the Bio Heat Transfer Equation (BHTE) on a collocated, smooth and non-orthogonal tridimensional mesh with hexahedral cells. The equation is solved on a curvilinear coordinate system fitting the grid. Simulated HIFU beam is generated by a 3-cm2 truncated spherical transducer with 40-mm natural focus and operating at 3 MHz. Tests are performed on two configurations with 6s-long sonication at an acoustic power of 32 W: 1) on a homogeneous matrix undergoing compression, dilatation, rotation or translation movements and 2) on the atrioventricular node (AVN) of a healthy beating heart model reconstructed from 4D CT-scan. Results/Discussion: In homogeneous volume (1), for static configuration, the maximum temperature reaches 69�C and the thermal lesion volume is 4.91 mm3. Depending on applied deformations, the maximum temperature varies from 55 to 70�C, and the lesion volume fluctuates from 0.05 to 5.51 mm3. In AVN (2), the maximum temperature reaches 83�C in static heart and the lesion volume is 18.74 mm3. Considering heart deformations and motions, temperature only reaches 46�C and no lesion is visible. Movements and deformations affect significantly simulation results in simple and more complex models. Neglecting heart movements and deformations induce overestimation of HIFU treatment efficacy. Thus, considering deformations and movements in modeling studies is required to provide accurate treatment strategies.
Published: 2020
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43. Development of a hifu treatment using a toroidal transducer for pancreatic adenocarcinoma: Preliminary in vivo study

Author: Cilleros, Celia, Dupré, Aurélien, Vincenot, Jérémy, Melodelima, David, Service d'Oncologie Chirurgicale [Lyon], Centre Léon Bérard [Lyon], Application des ultrasons à la thérapie (LabTAU), Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: [PHYS.MECA.VIBR]Physics [physics]/Mechanics [physics]/Vibrations [physics.class-ph], in vivo, Doppler, HIFU, pancreas, [PHYS.MECA.ACOU]Physics [physics]/Mechanics [physics]/Acoustics [physics.class-ph]
Abstract: International audience; Pancreatic adenocarcinoma is one of the most aggressive cancer. Regardless of the treatment used, the survival rate after 5 years is lower than 6%. Toroidal transducers can treat up to 60 cm3 of tissues in 370 seconds without the need to displace the HIFU probe. In this study, which included fourteen pigs, we evaluated in vivo the use of this device for treating the pancreas and peripancreatic vessels. The device was used intra-operatively. The transducer, working at 2.5MHz, has a toroidal shape with a radius of curvature of 70 mm focusing on a circle of 30 mm. An ultrasound-imaging probe working at 7.5 MHz was placed in the center of the HIFU transducer. Ablations were created in 440 seconds using an acoustic power of 85 or 100 watts. In total eight pigs were included in this study. Eight lesions were created in the pancreas and around peri-pancreatic vessels and observed immediately after treatment. Homogenous ablations were obtained in all cases and were confirmed histologically. The average diameter of the pancreatic ablations was 33.1 � 5.0 mm. These ablations were also homogeneous all around the peripancreatic artery and without occlusion as confirmed by Doppler examination. Using this toroidal HIFU transducer it is possible to treat the most challenging region of the pancreas and its surrounding vessels without any occlusion. This may allow to treat locally advanced pancreatic tumors which are the main contra-indication to curative resection.
Published: 2020
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44. High frequency elastic wave propagation in micro-elastography

Author: Laloy-Borgna, Gabrielle, Zorgani, Ali, Giammarinaro, Bruno, Catheline, Stefan, Application des ultrasons à la thérapie (LabTAU), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre Léon Bérard [Lyon]-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Jean le Rond d'Alembert (DALEMBERT), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS), Institut des Sciences de la Terre (ISTerre), and Institut national des sciences de l'Univers (INSU - CNRS)-Institut de recherche pour le développement [IRD] : UR219-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National de la Recherche Scientifique (CNRS)-Université Gustave Eiffel-Université Grenoble Alpes (UGA)
Subjects: [PHYS.MECA.VIBR]Physics [physics]/Mechanics [physics]/Vibrations [physics.class-ph], [PHYS.MECA.ACOU]Physics [physics]/Mechanics [physics]/Acoustics [physics.class-ph]
Abstract: International audience; We recently proposed an alternative elasticity measurement technique based on elastic wave propagation within a single cell. At this scale, waves at a frequency never reached in the field of elastography (15kHz), are captured using an ultrafast camera and a microscope. This technique is based on the local measurement of the speed of a shear wave, a type of elastic wave. By assuming an infinite and homogeneous elastic medium with respect to the wavelength, the shear modulus μ (elasticity) is estimated. These latter assumptions are discussed through experiments conducted in controlled elastic solids at micro-scale. The conclusion is that wave guide effects as well as viscosity are crucial for quantitative mapping of elasticity.
Published: 2020
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45. Développement d’un transducteur torique extracorporel et du principe de focalisation multi-torique en vue du traitement des adénocarcinomes mammaires par ultrasons focalisés de haute intensité

Author: Sanchez, Marine, Application des ultrasons à la thérapie (LabTAU), Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Lyon, and David Melodelima
Subjects: HIFU transducer, Toroidal transducer, Breast cancer, Transducteur HIFU, Traitement non invasif, Cancers du sein, Eletronical focusing, Non-invasive treatment, Transducteur torique, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology, Focalisation électronique
Abstract: High Intensity Focused Ultrasound (HIFU) is a technique commonly used in the medical field for the treatment of solid tumors. This therapeutic strategy allows for the localized destruction of biological tissue by temperature elevation. This manuscript describes the design and utilization of a therapeutic system and a novel, patented focalization technique for non-invasive treatment of mammary adenocarcinomas. In a first instance, an existing device that has been previously used for intraoperative treatment of hepatic metastases, and one that is currently undergoing clinical trials, was used. An ex vivo study on human mammary samples demonstrated the possibility of applying pressures on deep tissue layers in a non-invasive manner. Based on this study, the development of a new focalization modality was developed to provide larger treated volumes while yielding treatment times under a minute and eliminating the need for mechanical steering of the device. This new modality provided treated volumes 30% larger than those produced by the existing device with no changes to treatment time. Given the limitations of the existing device, in addition to the need of integrating the new focalization strategy, a new device was designed and specifically fabricated to meet the demands for treating mammary adenocarcinomas. The natural focalization of this novel transducer allows for depositing pressure deep in tissue. When the new focalization modality is used, a larger pressure field produces increased treated volumes. The performance provided by the novel probe was first characterized through modeling studies. These results were then validated through preliminary in vitro studies on hepatic tissues as well as in ex vivo studies on human mammary tissue samples. This work represents the first stage of development of a HIFU treatment for mammary adenocarcinomas using a novel, non-invasive and image-guided HIFU device that can be used manually; Les ultrasons focalisés de haute intensité (HIFU) sont reconnus et couramment utilisés dans le domaine médical pour le traitement des tumeurs solides. Cette technique permet une destruction localisée des tissus biologiques par élévation de la température. Ce manuscrit décrit la conception et l’utilisation d’un système thérapeutique et d’une nouvelle solution de focalisation brevetée, ayant pour objectif principal le traitement non invasif des adénocarcinomes mammaires. Dans un premier temps, un dispositif existant destiné au traitement surfacique des métastases hépatiques et en cours d’évaluation clinique a été utilisé. Une étude ex vivo sur échantillons humains de tissu mammaire a permis de montrer la possibilité de déposer de la pression en profondeur de façon non invasive. Basé sur cette étude, le développement d’une nouvelle modalité de focalisation a été entrepris dans le but d’élargir le volume traité sans impliquer des temps de traitement supérieur à la minute et des déplacements mécaniques du dispositif. Cette modalité a permis d’augmenter de 30 % le volume traité par le dispositif existant sans augmenter le temps de traitement. Compte tenu des limites du dispositif existant et de la nouvelle solution à intégrer, un nouveau dispositif a été conçu et fabriqué dans le but de répondre aux besoins des traitements des adénocarcinomes mammaires. La focalisation naturelle de ce transducteur permet de déposer de la pression en profondeur. Si la nouvelle modalité de focalisation électronique est utilisée, un champ de pression élargi est émis, permettant ainsi d’augmenter le volume de traitement. Les performances de cette sonde de traitement ont été évaluées numériquement puis validées lors d’expérimentations préliminaires in vitro sur des tissus hépatiques et lors d’une étude ex vivo sur des échantillons humains de tissu mammaire. Ces travaux représentent la première étape de l’élaboration d’un traitement HIFU pour traiter les adénocarcinomes mammaires avec un dispositif HIFU non-invasif, guidé par échographie et utilisable manuellement
Published: 2020

46. Secondary radiation force between two closely spaced acoustic bubbles

Author: Claude Inserra, Cyril Mauger, Philippe Blanc-Benon, Gabriel Regnault, Laboratoire de Mecanique des Fluides et d'Acoustique (LMFA), École Centrale de Lyon (ECL), Université de Lyon-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS), Application des ultrasons à la thérapie (LabTAU), Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Centre Léon Bérard [Lyon]-Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: Surface (mathematics), Physics, Scattering, Attenuation, Mechanics, 01 natural sciences, [PHYS.MECA.ACOU]Physics [physics]/Mechanics [physics]/Acoustics [physics.class-ph], 010305 fluids & plasmas, Physics::Fluid Dynamics, 0103 physical sciences, Secondary radiation, Microbubbles, Levitation, Liquid flow, [PHYS.MECA.MEFL]Physics [physics]/Mechanics [physics]/Fluid mechanics [physics.class-ph], 010306 general physics, ComputingMilieux_MISCELLANEOUS
Abstract: Two acoustic bubbles may attract or repel due to the secondary radiation force acting on them. We use here a dual-frequency levitation chamber in order to trap two oscillating microbubbles at close, fixed distance, and to perform measurements of the interaction force. We successfully compare our measurements to a commonly used theoretical model that assumes linear spherical oscillations, and disregards attenuation and multiple scattering between bubbles. The deviation from the model arises when nonspherical surface oscillations are triggered, leading to an additional hydrodynamic force induced by second-order liquid flow.
Published: 2020
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47. Using Sparse Array for 3D Passive Cavitation Imaging

Author: Audrey Sivadon, Jean-Christophe Béra, Bruno Gilles, Francois Varray, Barbara Nicolas, Application des ultrasons à la thérapie (LabTAU), Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM), Imagerie Ultrasonore, Centre de Recherche en Acquisition et Traitement de l'Image pour la Santé (CREATIS), Université Jean Monnet [Saint-Étienne] (UJM)-Hospices Civils de Lyon (HCL)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Jean Monnet [Saint-Étienne] (UJM)-Hospices Civils de Lyon (HCL)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Nicolas, Barbara, Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Hospices Civils de Lyon (HCL)-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Hospices Civils de Lyon (HCL)-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Materials science, [INFO.INFO-TS] Computer Science [cs]/Signal and Image Processing, business.industry, Acoustics, Sparse Array, Ultrasound, 3D Imaging, 01 natural sciences, 3. Good health, 030218 nuclear medicine & medical imaging, 03 medical and health sciences, 0302 clinical medicine, Sparse array, [INFO.INFO-TS]Computer Science [cs]/Signal and Image Processing, Cavitation, 0103 physical sciences, Cavitation Imaging, business, 010301 acoustics, ComputingMilieux_MISCELLANEOUS
Abstract: Cavitation is involved in a number of therapeutic applications of ultrasound (US) [1] [2] [3]. Monitoring these treatments requires real-time imaging of the cavitation activity during treatment. For this reason, Passive Acoustic Mapping (PAM) of cavitation has drawn an increasing attention during the past years [4] [5] [6] [7] [8].
Published: 2020
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48. Microstreaming induced by an acoustically excited gas bubble - experiments and comparison to theory

Author: Cleve, Sarah, Regnault, Gabriel, Doinikov, Alexander, Mauger, Cyril, Blanc-Benon, Philippe, Inserra, Claude, Laboratoire de Mecanique des Fluides et d'Acoustique (LMFA), École Centrale de Lyon (ECL), Université de Lyon-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS), Application des ultrasons à la thérapie (LabTAU), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre Léon Bérard [Lyon]-Institut National de la Santé et de la Recherche Médicale (INSERM), Mauger, Cyril, Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: [SPI.ACOU]Engineering Sciences [physics]/Acoustics [physics.class-ph], [SPI.ACOU] Engineering Sciences [physics]/Acoustics [physics.class-ph], [SPI]Engineering Sciences [physics], [SPI] Engineering Sciences [physics], ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2020

49. Full-length 5'RACE identifies all major HBV transcripts in HBV-infected hepatocytes and patient serum

Author: Michel Rivoire, Fleur Chapus, Audrey Diederichs, Antoine Alam, Laurent Fraisse, Kara Carter, Barbara Testoni, Bernd Stadelmayer, Fabien Zoulim, Gregory Neveu, Centre de Recherche en Cancérologie de Lyon (UNICANCER/CRCL), Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon, Application des ultrasons à la thérapie (LabTAU), and Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: 0301 basic medicine, Hepatitis B virus, viruses, [SDV]Life Sciences [q-bio], Biology, medicine.disease_cause, Genome, 03 medical and health sciences, 0302 clinical medicine, Rapid amplification of cDNA ends, Transcription (biology), medicine, Humans, Hepatology, Gene Expression Profiling, RNA, cccDNA, Hepatitis B, medicine.disease, Virology, digestive system diseases, 3. Good health, HBx, 030104 developmental biology, DNA, Viral, Hepatocytes, 030211 gastroenterology & hepatology, Transcriptome, Nucleic Acid Amplification Techniques
Abstract: Background & Aims Covalently closed circular DNA (cccDNA) is the episomal form of the HBV genome that stably resides in the nucleus of infected hepatocytes. cccDNA is the template for the transcription of 6 major viral RNAs, i.e. preC, pg, preS1/2, S and HBx RNA. All viral transcripts share the same 3' end and are all to various degrees subsets of each other. Especially under infection conditions, it has been difficult to study in depth the transcription of the different viral transcripts. We thus wanted to develop a method with which we could easily detect the full spectrum of viral RNAs in any lab. Methods We set up an HBV full-length 5'RACE (rapid amplification of cDNA ends) method with which we measured and characterized the full spectrum of viral RNAs in cell culture and in chronically infected patients. Results In addition to canonical HBx transcripts coding for full-length X, we identified shorter HBx transcripts potentially coding for short X proteins. We showed that interferon-β treatment leads to a strong reduction of preC and pgRNAs but has only a moderate effect on the other viral transcripts. We found pgRNA, 1 spliced pgRNA variant and a variety of HBx transcripts associated with viral particles generated by HepAD38 cells. The different HBx RNAs are both capped and uncapped. Lastly, we identified 3 major categories of circulating RNA species in patients with chronic HBV infection: pgRNA, spliced pgRNA variants and HBx. Conclusions This HBV full-length 5'RACE method should significantly contribute to the understanding of HBV transcription during the course of infection and therapy and may guide the development of novel therapies aimed at targeting cccDNA. Lay summary Especially under infection conditions, it has been difficult to study the different hepatitis B virus transcripts in depth. This study introduces a new method that can be used in any standard lab to discriminate all hepatitis B viral transcripts in cell culture and in the serum of patients.
Published: 2020
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50. Safety of endoscopic retrograde cholangiopancreatography in the pediatric population: a multicenter study

Author: Stéphane Koch, Nicolas Williet, Clémence Mercier, Pierre Henri Deprez, Marion Schaefer, Isabelle Scheers, Jean-Phillippe Le Mouel, Frédéric Prat, Marc Barthet, Aline Derosiere, Jean-Baptiste Chevaux, Julien Branche, Jean-Christophe Valats, Phillipe Grandval, Thierry Ponchon, Isabelle Boytchev, Geoffroy Vanbiervliet, Caroline Chabot, Clement Fortier Beaulieu, Nicolas Musquer, Emmanuel Coron, Mathieu Pioche, Timothée Wallenhorst, Franz Rudler, Aurélie Comte, Jean-Michel Gonzalez, Arthur Belle, Eliane Albuisson, Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy), Application des ultrasons à la thérapie (LabTAU), Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM), Hôpital Edouard Herriot [CHU - HCL], Hospices Civils de Lyon (HCL), Service d'hépatologie et de gastroentérologie [Hôpital Saint-Joseph - Marseille], Aix Marseille Université (AMU)-Hôpital Saint-Joseph [Marseille], Service d'Hépato-gastro-entérologie [APHP Kremlin-Bicêtre], AP-HP Hôpital Bicêtre (Le Kremlin-Bicêtre), Centre Hospitalier Universitaire de Nice (CHU Nice), Hôpital Cochin [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Service de Gastro-entérologie [CHU Cochin], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Cochin [AP-HP], Hôpital Claude Huriez [Lille], CHU Lille, Centre de Recherche en Cancérologie de Marseille (CRCM), Aix Marseille Université (AMU)-Institut Paoli-Calmettes, Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), Université de Montpellier (UM), Hôpital Sud [CHU Rennes], CHU Pontchaillou [Rennes], Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon), Service de chirurgie pédiatrique [CHU Besançon], Centre Hospitalier Universitaire de Saint-Etienne [CHU Saint-Etienne] (CHU ST-E), Centre hospitalier universitaire de Nantes (CHU Nantes), Service d'Hépato Gastroenterologie [CHU Amiens-Picardie], CHU Amiens-Picardie, and Cliniques Universitaires Saint-Luc [Bruxelles]
Subjects: medicine.medical_specialty, medicine.medical_treatment, Perforation (oil well), Liver transplantation, MESH: Catheterization, Catheterization, MESH: Child, medicine, Humans, Neonatal cholestasis, Child, Retrospective Studies, Cholangiopancreatography, Endoscopic Retrograde, Univariate analysis, MESH: Humans, Endoscopic retrograde cholangiopancreatography, medicine.diagnostic_test, business.industry, MESH: Infant, Newborn, Gastroenterology, Infant, Newborn, MESH: Retrospective Studies, [SDV.MHEP.HEG]Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology, Odds ratio, medicine.disease, Surgery, MESH: Pancreatitis, Choledocholithiasis, Pancreatitis, MESH: Cholangiopancreatography, Endoscopic Retrograde, MESH: Choledocholithiasis, business, Complication
Abstract: Introduction The aims of this retrospective multicenter study were to assess the technical success and adverse events of endoscopic retrograde cholangiopancreatography (ERCP) procedures in children in French and Belgian centers. Methods All children aged 1 day to 17 years who underwent ERCP between January 2008 and March 2019 in 15 tertiary care hospitals were retrospectively included. Results 271 children underwent 470 ERCP procedures. Clinical long-term follow-up was available for 72 % of our patients (340/470 procedures). The median age at intervention was 10.9 years. ERCP was therapeutic in 90 % (423/470) and diagnostic in cases of neonatal cholestasis in 10 % of the patients. The most common biliary indication was choledocholithiasis; the most common pancreatic indication was chronic pancreatitis. Biliary cannulation was successful in 92 % of cases (270/294); pancreatic cannulation in 96 % of cases (169/176); and planned therapeutic procedures in 92 % of cases (388/423). The overall complication rate was 19 % (65/340). The most common complication was post-ERCP pancreatitis (PEP) in 12 % of cases (40/340) and sepsis in 5 % (18/340). On univariate analyses, pancreatic stent removal was protective against PEP (odds ratio [OR] 0.1, 95 % confidence interval [CI] 0.01 – 0.75; P = 0.03), and sepsis was associated with history of liver transplantation (OR 7.27, 95 %CI 1.7 – 31.05; P = 0.01). Five patients had post-ERCP hemorrhage and two had intestinal perforation. All complications were managed with supportive medical care. There was no procedure-related mortality. Conclusion Our cohort demonstrates that ERCP can be performed safely with high success rates in many pancreaticobiliary diseases of children. The rate of adverse events was similar to that in previous reports.
Published: 2020
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