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29 results on '"Anupama, Atashi"'

1. The Genome of the Kinetoplastid Parasite, Leishmania major

Author

Ivens, Alasdair C., Peacock, Christopher S., Worthey, Elizabeth A., Murphy, Lee, Aggarwal, Gautam, Berriman, Matthew, Sisk, Ellen, Rajandream, Marie-Adele, Adlem, Ellen, Aert, Rita, Anupama, Atashi, Apostolou, Zina, Attipoe, Philip, Bason, Nathalie, Bauser, Christopher, Beck, Alfred, Beverley, Stephen M., Bianchettin, Gabriella, Borzym, Katja, Bothe, Gordana, Bruschi, Carlo V., Collins, Matt, Cadag, Eithon, Ciarloni, Laura, Clayton, Christine, Cronin, Ann, Cruz, Angela K., Davies, Robert M., De Gaudenzi, Javier, Dobson, Deborah E., Duesterhoeft, Andreas, Fazelina, Gholam, Fosker, Nigel, Frasch, Alberto Carlos, Fraser, Audrey, Fuchs, Monika, Gabel, Claudia, Goble, Arlette, Goffeau, André, Harris, David, Hertz-Fowler, Christiane, Hilbert, Helmut, Horn, David, Huang, Yiting, Klages, Sven, Knights, Andrew, Kube, Michael, Larke, Natasha, Litvin, Lyudmila, Lord, Angela, Louie, Tin, Marra, Marco, Masuy, David, Matthews, Keith, Michaeli, Shulamit, Mottram, Jeremy C., Müller-Auer, Silke, Munden, Heather, Nelson, Siri, Norbertczak, Halina, Oliver, Karen, O'Neil, Susan, Pentony, Martin, Pohl, Thomas M., Price, Claire, Purnelle, Bénédicte, Quail, Michael A., Rabbinowitsch, Ester, Reinhardt, Richard, Rieger, Michael, Rinta, Joel, Robben, Johan, Robertson, Laura, Ruiz, Jeronimo C., Rutter, Simon, Saunders, David, Schäfer, Melanie, Schein, Jacquie, Schwartz, David C., Seeger, Kathy, Seyler, Amber, Sharp, Sarah, Shin, Heesun, Sivam, Dhileep, Squares, Rob, Squares, Steve, Tosato, Valentina, Vogt, Christy, Volckaert, Guido, Wambutt, Rolf, Warren, Tim, Wedler, Holger, Woodward, John, Zhou, Shiguo, Zimmermann, Wolfgang, Smith, Deborah F., Blackwell, Jenefer M., Stuart, Kenneth D., Barrell, Bart, and Myler, Peter J.

Published
2005

2. Comparative Genomics of Trypanosomatid Parasitic Protozoa

Author

El-Sayed, Najib M., Myler, Peter J., Blandin, Gaëlle, Berriman, Matthew, Crabtree, Jonathan, Aggarwal, Gautam, Caler, Elisabet, Renauld, Hubert, Worthey, Elizabeth A., Hertz-Fowler, Christiane, Ghedin, Elodie, Peacock, Christopher, Bartholomeu, Daniella C., Haas, Brian J., Tran, Anh-Nhi, Wortman, Jennifer R., Angiuoli, Samuel, Anupama, Atashi, Badger, Jonathan, Bringaud, Frederic, Cadag, Eithon, Carlton, Jane M., Cerqueira, Gustavo C., Creasy, Todd, Delcher, Arthur L., Djikeng, Appolinaire, Embley, T. Martin, Hauser, Christopher, Ivens, Alasdair C., Kummerfeld, Sarah K., Pereira-Leal, Jose B., Nilsson, Daniel, Peterson, Jeremy, Salzberg, Steven L., Shallom, Joshua, Silva, Joana C., Sundaram, Jaideep, Westenberger, Scott, White, Owen, Melville, Sara E., Donelson, John E., Andersson, Björn, Stuart, Kenneth D., and Hall, Neil

Published
2005

3. The Genome Sequence of Trypanosoma cruzi, Etiologic Agent of Chagas Disease

Author

El-Sayed, Najib M., Myler, Peter J., Bartholomeu, Daniella C., Nilsson, Daniel, Aggarwal, Gautam, Tran, Anh-Nhi, Ghedin, Elodie, Worthey, Elizabeth A., Delcher, Arthur L., Blandin, Gaëlle, Westenberger, Scott J., Caler, Elisabet, Cerqueira, Gustavo C., Anupama, Atashi, Arner, Erik, Åslund, Lena, Attipoe, Philip, Bontempi, Esteban, Bringaud, Frédéric, Burton, Peter, Cadag, Eithon, Campbell, David A., Carrington, Mark, Crabtree, Jonathan, Darban, Hamid, da Silveira, Jose Franco, de Jong, Pieter, Edwards, Kimberly, Englund, Paul T., Fazelina, Gholam, Feldblyum, Tamara, Ferella, Marcela, Frasch, Alberto Carlos, Gull, Keith, Horn, David, Hou, Lihua, Huang, Yiting, Kindlund, Ellen, Klingbeil, Michele, Kluge, Sindy, Koo, Hean, Lacerda, Daniela, Levin, Mariano J., Lorenzi, Hernan, Louie, Tin, Machado, Carlos Renato, McCulloch, Richard, McKenna, Alan, Mizuno, Yumi, Mottram, Jeremy C., Nelson, Siri, Ochaya, Stephen, Osoegawa, Kazutoyo, Pai, Grace, Parsons, Marilyn, Pentony, Martin, Pettersson, Ulf, Pop, Mihai, Ramirez, Jose Luis, Rinta, Joel, Robertson, Laura, Salzberg, Steven L., Sanchez, Daniel O., Seyler, Amber, Sharma, Reuben, Shetty, Jyoti, Simpson, Anjana J., Sisk, Ellen, Tammi, Martti T., Tarleton, Rick, Teixeira, Santuza, Van Aken, Susan, Vogt, Christy, Ward, Pauline N., Wickstead, Bill, Wortman, Jennifer, White, Owen, Fraser, Claire M., Stuart, Kenneth D., and Andersson, Björn

Published
2005

4. The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas disease

Author

Sayed, Najib M. El-, Myler, Peter J., Bartholomeu, Daniela C., Nilsson, Daniel, Aggarwal, Gautam, Tran, Anh-Nhi, Ghedin, Elodie, Worthey, Elizabeth A., Delcher, Arthur L., Blandin, Gaelle, Westenberger, Scott J., Caler, Elisabet, Cerqueira, Gustavo C., Branche, Carole, Haas, Brian, Anupama, Atashi, Arner, Erik, Aslund, Lena, Attipoe, Philip, Bontempi, Esteban, Bringaud, Frederic, Burton, Peter, Cadag, Eithon, Campbell, David A., Carrington, Mark, Crabtree, Jonathan, Darban, Hamid, da Silveira, Jose Franco, de Jong, Pieter, Edwards, Kimberly, Englund, Paul T., Fazelina, Gholam, Feldblyum, Tamara, Ferella, Marcela, Frasch, Alberto Carlos, Gull, Keith, Horn, David, Hou, Lihua, Huang, Yiting, Kindlund, Ellen, Klingbeil, Michele, Kluge, Sindy, Koo, Hean, Lacerda, Daniela, Levin, Mariano J., Lorenzi, Hernan, Louie, Tin, Machado, Carlos Renato, McCulloch, Richard, McKenna, Alan, Mizuno, Yumi, Mottram, Jeremy C., Nelson, Siri, Ochaya, Stephen, Osoegawa, Kazutoyo, Pai, Grace, Parsons, Marilyn, Pentony, Martin, Pettersson, Ulf, Pop, Mihai, Ramirez, Jose Luis, Rinta, Joel, Robertson, Laura, Salzberg, Steven L., Sanchez, Daniel O., Seyler, Amber, Sharma, Reuben, Shetty, Jyoti, Simpson, Anjana J., Sisk, Ellen, Tammi, Martti T., Tarleton, Rick, Teixeira, Santuza, Van Aken, Susan, Vogt, Christy, Ward, Pauline N., Wickstead, Bill, Wortman, Jennifer, White, Owen, Fraser, Claire M., Stuart, Kenneth D., and Andersson, Bjorn

Subjects
Trypanosoma cruzi -- Genetic aspects, Science and technology
Abstract

Whole-genome sequencing of the protozoan pathogen Trypanosoma cruzi revealed that the diploid genome contains a predicted 22,570 proteins encoded by genes, of which 12,570 represent allelic pairs. Over 50% of the genome consists of repeated sequences, such as retrotransposons and genes for large families of surface molecules, which include trans-sialidases, mucins, gp63s, and a large novel family (>1300 copies) of mucin-associated surface protein (MASP) genes. Analyses of the T. cruzi, T. brucei, and Leishmania major (Tritryp) genomes imply differences from other eukaryotes in DNA repair and initiation of replication and reflect their unusual mitochondrial DNA. Although the Tritryp lack several classes of signaling molecules, their kinomes contain a large and diverse set of protein kinases and phosphatases; their size and diversity imply previously unknown interactions and regulatory processes, which may be targets for intervention., Trypanosoma cruzi causes Chagas disease in humans. Acute infection can be lethal, but the disease usually evolves into a chronic stage, accompanied in 25 to 30% of cases by severe [...]

Published
2005

5. Comparative genomics of trypanosomatid parasitic protozoa

Author

Sayed, Najib M. El-, Myler, Peter J., Blandin, Gaelle, Berriman, Matthew, Crabtree, Jonathan, Aggarwal, Gautam, Caler, Elisabet, Renauld, Hubert, Worthey, Elizabeth A., Hertz-Fowler, Christiane, Ghedin, Elodie, Peacock, Christopher, Bartholomeu, Daniella C., Haas, Brian J., Tran, Anh-Nhi, Wortman, Jennifer R., Alsmark, U. Cecilia M., Angiuoli, Samuel, Anupama, Atashi, Badger, Jonathan, Bringaud, Frederic, Cadag, Eithon, Carlton, Jane M., Cerqueira, Gustavo C., Creasy, Todd, Delcher, Arthur L., Djikeng, Appolinaire, Embley, T. Martin, Hauser, Christopher, Ivens, Alasdair C., Kummerfeld, Sarah K., Pereira-Leal, Jose B., Nilsson, Daniel, Peterson, Jeremy, Salzberg, Steven L., Shallom, Joshua, Silva, Joana C., Sundaram, Jaideep, Westenberger, Scott, White, Owen, Melville, Sara E., Donelson, John E., Andersson, Bjorn, Stuart, Kenneth D., and Hall, Neil

Subjects
Leishmaniasis -- Genetic aspects, Trypanosoma cruzi -- Genetic aspects, Trypanosoma brucei -- Genetic aspects, Science and technology, Genetic aspects
Abstract

A comparison of gene content and genome architecture of Trypanosoma brucei, Trypanosoma cruzi, and Leishmania major, three related pathogens with different life cycles and disease pathology, revealed a conserved core proteome of about 6200 genes in large syntenic polycistronic gene clusters. Many species-specific genes, especially large surface antigen families, occur at nonsyntenic chromosome-internal and subtelomeric regions. Retroelements, structural RNAs, and gene family expansion are often associated with syntenic discontinuities that--along with gene divergence, acquisition and loss, and rearrangement within the syntenic regions--have shaped the genomes of each parasite. Contrary to recent reports, our analyses reveal no evidence that these species are descended from an ancestor that contained a photosynthetic endosymbiont., The protozoan pamogens Leishmania major, Trypanosoma cruzi, and Trypanosoma brucei (family Trypanosomatidae, order Kinetoplastida) collectively cause disease and death in millions of humans and countless infections in other mammals, primarily [...]

Published
2005

6. Whole blood transcriptome changes following controlled human malaria infection in malaria pre-exposed volunteers correlate with parasite prepatent period

Author

Rothen, Julian, primary, Murie, Carl, additional, Carnes, Jason, additional, Anupama, Atashi, additional, Abdulla, Salim, additional, Chemba, Mwajuma, additional, Mpina, Maxmillian, additional, Tanner, Marcel, additional, Lee Sim, B. Kim, additional, Hoffman, Stephen L., additional, Gottardo, Raphael, additional, Daubenberger, Claudia, additional, and Stuart, Ken, additional

Published
2018
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10. Genome and phylogenetic analyses of Trypanosoma evansi reveal extensive similarity to T. brucei and multiple independent origins for dyskinetoplasty

Author

Carnes, Jason, Anupama, Atashi, Balmer, Olivier, Jackson, Andrew, Lewis, Michael, Brown, Rob, Cestari, Igor, Desquesnes, Marc, Gendrin, Claire, Hertz-Fowler, Christiane, Imamura, Hideo, Ivens, Alasdair, Koreny, Ludek, Lai, De-Hua, MacLeod, Annette, McDermott, Suzanne M., Merritt, Chris, Monnerat, Severine, Moon, Wonjong, Myler, Peter, Phan, Isabelle, Ramasamy, Gowthaman, Sivam, Dhileep, Lun, Zhao-Rong, Lukes, Julius, Stuart, Ken, Schnaufer, Achim, Carnes, Jason, Anupama, Atashi, Balmer, Olivier, Jackson, Andrew, Lewis, Michael, Brown, Rob, Cestari, Igor, Desquesnes, Marc, Gendrin, Claire, Hertz-Fowler, Christiane, Imamura, Hideo, Ivens, Alasdair, Koreny, Ludek, Lai, De-Hua, MacLeod, Annette, McDermott, Suzanne M., Merritt, Chris, Monnerat, Severine, Moon, Wonjong, Myler, Peter, Phan, Isabelle, Ramasamy, Gowthaman, Sivam, Dhileep, Lun, Zhao-Rong, Lukes, Julius, Stuart, Ken, and Schnaufer, Achim

Abstract

Two key biological features distinguish Trypanosoma evansi from the T. brucei group: independence from the tsetse fly as obligatory vector, and independence from the need for functional mitochondrial DNA (kinetoplast or kDNA). In an effort to better understand the molecular causes and consequences of these differences, we sequenced the genome of an akinetoplastic T. evansi strain from China and compared it to the T. b. brucei reference strain. The annotated T. evansi genome shows extensive similarity to the reference, with 94.9% of the predicted T. b. brucei coding sequences (CDS) having an ortholog in T. evansi, and 94.6% of the non-repetitive orthologs having a nucleotide identity of 95% or greater. Interestingly, several procyclin-associated genes (PAGs) were disrupted or not found in this T. evansi strain, suggesting a selective loss of function in the absence of the insect life-cycle stage. Surprisingly, orthologous sequences were found in T. evansi for all 978 nuclear CDS predicted to represent the mitochondrial proteome in T. brucei, although a small number of these may have lost functionality. Consistent with previous results, the F1FO-ATP synthase c subunit was found to have an A281 deletion, which is involved in generation of a mitochondrial membrane potential in the absence of kDNA. Candidates for CDS that are absent from the reference genome were identified in supplementary de novo assemblies of T. evansi reads. Phylogenetic analyses show that the sequenced strain belongs to a dominant group of clonal T. evansi strains with worldwide distribution that also includes isolates classified as T. equiperdum. At least three other types of T. evansi or T. equiperdum have emerged independently. Overall, the elucidation of the T. evansi genome sequence reveals extensive similarity of T. brucei and supports the contention that T. evansi should be classified as a subspecies of T. brucei.

Published
2015

12. Iron superoxide dismutases in eukaryotic pathogens: new insights from Apicomplexa andTrypanosomastructures

Author

Phan, Isabelle Q. H., primary, Davies, Douglas R., additional, Moretti, Nilmar Silvio, additional, Shanmugam, Dhanasekaran, additional, Cestari, Igor, additional, Anupama, Atashi, additional, Fairman, James W., additional, Edwards, Thomas E., additional, Stuart, Kenneth, additional, Schenkman, Sergio, additional, and Myler, Peter J., additional

Published
2015
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13. Genome and Phylogenetic Analyses of Trypanosoma evansi Reveal Extensive Similarity to T. brucei and Multiple Independent Origins for Dyskinetoplasty

Author

Carnes, Jason, primary, Anupama, Atashi, additional, Balmer, Oliver, additional, Jackson, Andrew, additional, Lewis, Michael, additional, Brown, Rob, additional, Cestari, Igor, additional, Desquesnes, Marc, additional, Gendrin, Claire, additional, Hertz-Fowler, Christiane, additional, Imamura, Hideo, additional, Ivens, Alasdair, additional, Kořený, Luděk, additional, Lai, De-Hua, additional, MacLeod, Annette, additional, McDermott, Suzanne M., additional, Merritt, Chris, additional, Monnerat, Severine, additional, Moon, Wonjong, additional, Myler, Peter, additional, Phan, Isabelle, additional, Ramasamy, Gowthaman, additional, Sivam, Dhileep, additional, Lun, Zhao-Rong, additional, Lukeš, Julius, additional, Stuart, Ken, additional, and Schnaufer, Achim, additional

Published
2015
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14. Inositol polyphosphate multikinase regulation of Trypanosoma bruceilife stage development

Author

Cestari, Igor, Anupama, Atashi, and Stuart, Kenneth

Abstract

The regulation of Trypanosoma bruceilife stage development remains unclear. Inositol polyphosphate multikinase regulates the development of mammalian bloodforms to insect stages that normally develop in flies. Specific inositol phosphates, perhaps as second messengers, interact with proteins of the regulatory network that controls development.

Published
2018
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20. Trypanosoma brucei Mitochondrial Ribosomes

Author

Ziáková, Alena, primary, Panigrahi, Aswini K., additional, Dalley, Rachel A., additional, Acestor, Nathalie, additional, Anupama, Atashi, additional, Ogata, Yuko, additional, Myler, Peter J., additional, and Stuart, Kenneth, additional

Published
2008
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21. Mitochondrial Complexes in Trypanosoma brucei

Author

Panigrahi, Aswini K., primary, Zíková, Alena, additional, Dalley, Rachel A., additional, Acestor, Nathalie, additional, Ogata, Yuko, additional, Anupama, Atashi, additional, Myler, Peter J., additional, and Stuart, Kenneth D., additional

Published
2008
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22. The Genome of the Kinetoplastid Parasite,Leishmania major

Author

Ivens, Alasdair C., primary, Peacock, Christopher S., additional, Worthey, Elizabeth A., additional, Murphy, Lee, additional, Aggarwal, Gautam, additional, Berriman, Matthew, additional, Sisk, Ellen, additional, Rajandream, Marie-Adele, additional, Adlem, Ellen, additional, Aert, Rita, additional, Anupama, Atashi, additional, Apostolou, Zina, additional, Attipoe, Philip, additional, Bason, Nathalie, additional, Bauser, Christopher, additional, Beck, Alfred, additional, Beverley, Stephen M., additional, Bianchettin, Gabriella, additional, Borzym, Katja, additional, Bothe, Gordana, additional, Bruschi, Carlo V., additional, Collins, Matt, additional, Cadag, Eithon, additional, Ciarloni, Laura, additional, Clayton, Christine, additional, Coulson, Richard M. R., additional, Cronin, Ann, additional, Cruz, Angela K., additional, Davies, Robert M., additional, De Gaudenzi, Javier, additional, Dobson, Deborah E., additional, Duesterhoeft, Andreas, additional, Fazelina, Gholam, additional, Fosker, Nigel, additional, Frasch, Alberto Carlos, additional, Fraser, Audrey, additional, Fuchs, Monika, additional, Gabel, Claudia, additional, Goble, Arlette, additional, Goffeau, André, additional, Harris, David, additional, Hertz-Fowler, Christiane, additional, Hilbert, Helmut, additional, Horn, David, additional, Huang, Yiting, additional, Klages, Sven, additional, Knights, Andrew, additional, Kube, Michael, additional, Larke, Natasha, additional, Litvin, Lyudmila, additional, Lord, Angela, additional, Louie, Tin, additional, Marra, Marco, additional, Masuy, David, additional, Matthews, Keith, additional, Michaeli, Shulamit, additional, Mottram, Jeremy C., additional, Müller-Auer, Silke, additional, Munden, Heather, additional, Nelson, Siri, additional, Norbertczak, Halina, additional, Oliver, Karen, additional, O'Neil, Susan, additional, Pentony, Martin, additional, Pohl, Thomas M., additional, Price, Claire, additional, Purnelle, Bénédicte, additional, Quail, Michael A., additional, Rabbinowitsch, Ester, additional, Reinhardt, Richard, additional, Rieger, Michael, additional, Rinta, Joel, additional, Robben, Johan, additional, Robertson, Laura, additional, Ruiz, Jeronimo C., additional, Rutter, Simon, additional, Saunders, David, additional, Schäfer, Melanie, additional, Schein, Jacquie, additional, Schwartz, David C., additional, Seeger, Kathy, additional, Seyler, Amber, additional, Sharp, Sarah, additional, Shin, Heesun, additional, Sivam, Dhileep, additional, Squares, Rob, additional, Squares, Steve, additional, Tosato, Valentina, additional, Vogt, Christy, additional, Volckaert, Guido, additional, Wambutt, Rolf, additional, Warren, Tim, additional, Wedler, Holger, additional, Woodward, John, additional, Zhou, Shiguo, additional, Zimmermann, Wolfgang, additional, Smith, Deborah F., additional, Blackwell, Jenefer M., additional, Stuart, Kenneth D., additional, Barrell, Bart, additional, and Myler, Peter J., additional

Published
2005
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24. Trypanosoma bruceiTb927.2.6100 Is an Essential Protein Associated with Kinetoplast DNA

Author

Beck, Kirsten, Acestor, Nathalie, Schulfer, Anjelique, Anupama, Atashi, Carnes, Jason, Panigrahi, Aswini K., and Stuart, Ken

Abstract

ABSTRACTThe mitochondrial DNA of trypanosomatid protozoa consists of a complex, intercatenated network of tens of maxicircles and thousands of minicircles. This structure, called kinetoplast DNA (kDNA), requires numerous proteins and multiprotein complexes for replication, segregation, and transcription. In this study, we used a proteomic approach to identify proteins that are associated with the kDNA network. We identified a novel protein encoded by Tb927.2.6100 that was present in a fraction enriched for kDNA and colocalized the protein with kDNA by fluorescence microscopy. RNA interference (RNAi) knockdown of its expression resulted in a growth defect and changes in the proportion of kinetoplasts and nuclei in the cell population. RNAi also resulted in shrinkage and loss of the kinetoplasts, loss of maxicircle and minicircle components of kDNA at similar rates, and (perhaps secondarily) loss of edited and pre-edited mRNA. These results indicate that the Tb927.2.6100 protein is essential for the maintenance of kDNA.

Published
2013
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25. Trypanosoma brucei Mitochondrial Ribosomes: Affinity Purification and Component Identification by Mass Spectrometry

Author

Zíková, Alena, Panigrahi, Aswini K., Dalley, Rachel A., Acestor, Nathalie, Anupama, Atashi, Ogata, Yuko, Myler, Peter J., and Stuart, Kenneth

Abstract

Although eukaryotic mitochondrial (mt) ribosomes evolved from a putative prokaryotic ancestor their compositions vary considerably among organisms. We determined the protein composition of tandem affinity-purified Trypanosoma brucei mt ribosomes by mass spectrometry and identified 133 proteins of which 77 were associated with the large subunit and 56 were associated with the small subunit. Comparisons with bacterial and mammalian mt ribosomal proteins identified T. brucei mt homologs of L2–4, L7/12, L9, L11, L13–17, L20–24, L27–30, L33, L38, L43, L46, L47, L49, L52, S5, S6, S8, S9, S11, S15–18, S29, and S34, although the degree of conservation varied widely. Sequence characteristics of some of the component proteins indicated apparent functions in rRNA modification and processing, protein assembly, and mitochondrial metabolism implying possible additional roles for these proteins. Nevertheless most of the identified proteins have no homology outside Kinetoplastida implying very low conservation and/or a divergent function in kinetoplastid mitochondria.

Published
2008

26. Trypanosoma bruceiMitochondrial Ribosomes

Author

Ziáková, Alena, Panigrahi, Aswini K., Dalley, Rachel A., Acestor, Nathalie, Anupama, Atashi, Ogata, Yuko, Myler, Peter J., and Stuart, Kenneth

Abstract

Although eukaryotic mitochondrial (mt) ribosomes evolved from a putative prokaryotic ancestor their compositions vary considerably among organisms. We determined the protein composition of tandem affinity-purified Trypanosoma bruceimt ribosomes by mass spectrometry and identified 133 proteins of which 77 were associated with the large subunit and 56 were associated with the small subunit. Comparisons with bacterial and mammalian mt ribosomal proteins identified T. bruceimt homologs of L2–4, L7/12, L9, L11, L13–17, L20–24, L27–30, L33, L38, L43, L46, L47, L49, L52, S5, S6, S8, S9, S11, S15–18, S29, and S34, although the degree of conservation varied widely. Sequence characteristics of some of the component proteins indicated apparent functions in rRNA modification and processing, protein assembly, and mitochondrial metabolism implying possible additional roles for these proteins. Nevertheless most of the identified proteins have no homology outside Kinetoplastida implying very low conservation and/or a divergent function in kinetoplastid mitochondria.

Published
2008
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27. Genome and phylogenetic analyses of Trypanosoma evansi reveal extensive similarity to T. brucei and multiple independent origins for dyskinetoplasty

Author

Carnes, Jason, Anupama, Atashi, Balmer, Oliver, Jackson, Andrew, Lewis, Michael, Brown, Rob, Cestari, Igor, Desquesnes, Marc, Gendrin, Claire, Hertz-Fowler, Christiane, Imamura, Hideo, Ivens, Alasdair, Kořený, Luděk, Lai, De-Hua, MacLeod, Annette, McDermott, Suzanne M., Merritt, Chris, Monnerat, Severine, Moon, Wonjong, Myler, Peter, Phan, Isabelle, Ramasamy, Gowthaman, Sivam, Dhileep, Lun, Zhao-Rong, Lukeš, Julius, Stuart, Ken, and Schnaufer, Achim

Subjects
3. Good health

28. Trypanosoma bruceiMitochondrial Respiratome: Composition and Organization in Procyclic Form*

Author

Acestor, Nathalie, Zíková, Alena, Dalley, Rachel A., Anupama, Atashi, Panigrahi, Aswini K., and Stuart, Kenneth D.

Abstract

The mitochondrial respiratory chain is comprised of four different protein complexes (I–IV), which are responsible for electron transport and generation of proton gradient in the mitochondrial intermembrane space. This proton gradient is then used by FoF1-ATP synthase (complex V) to produce ATP by oxidative phosphorylation. In this study, the respiratory complexes I, II, and III were affinity purified from Trypanosoma bruceiprocyclic form cells and their composition was determined by mass spectrometry. The results along with those that we previously reported for complexes IV and V showed that the respiratome of Trypanosomais divergent because many of its proteins are unique to this group of organisms. The studies also identified two mitochondrial subunit proteins of respiratory complex IV that are encoded by edited RNAs. Proteomics data from analyses of complexes purified using numerous tagged component proteins in each of the five complexes were used to generate the first predicted protein-protein interaction network of the Trypanosoma bruceirespiratory chain. These results provide the first comprehensive insight into the unique composition of the respiratory complexes in Trypanosoma brucei, an early diverged eukaryotic pathogen.

Published
2011
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29. Inositol polyphosphate multikinase regulation of Trypanosoma brucei life stage development.

Author

Cestari I, Anupama A, and Stuart K

Subjects
Animals, Energy Metabolism, Inositol Phosphates metabolism, Life Cycle Stages physiology, Parasites metabolism, Phosphorylation, Signal Transduction, Trypanosoma brucei brucei genetics, Trypanosoma brucei brucei growth & development, Phosphotransferases (Alcohol Group Acceptor) metabolism, Phosphotransferases (Alcohol Group Acceptor) physiology, Trypanosoma brucei brucei metabolism
Abstract

Many cellular processes change during the Trypanosoma brucei life cycle as this parasite alternates between the mammalian host and tsetse fly vector. We show that the inositol phosphate pathway helps regulate these developmental changes. Knockdown of inositol polyphosphate multikinase (IPMK), which phosphorylates Ins(1,4,5)P3 and Ins(1,3,4,5)P4, resulted in changes in bloodstream forms that are characteristic of insect stage procyclic forms. These changes include expression of the procyclic surface coat, up-regulation of RNA-binding proteins that we show to regulate stage-specific transcripts, and activation of oxidative phosphorylation with increased ATP production in bloodstream forms. These changes were accompanied by development of procyclic morphology, which also occurred by the expression of a catalytically inactive IPMK, implying that regulation of these processes entails IPMK activity. Proteins involved in signaling, protein synthesis and turnover, and metabolism were affinity-enriched with the IPMK substrate or product. Developmental changes associated with IPMK knockdown or catalytic inactivation reflected processes that are enriched with inositol phosphates, and chemical and genetic perturbation of these processes affected T. brucei development. Hence, IPMK helps regulate T. brucei development, perhaps by affecting inositol phosphate interactions with proteins of the regulatory network that controls energy metabolism and development.

Published
2018
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