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Abstract Objective The purpose of the present study was to explore the effect of plateau duration in eliciting masseter vestibular evoked myogenic potentials (mVEMPs) in healthy individuals with hearing sensitivity ≤ 15dBHL. Method A within-subjects design was utilized in the study. Therefore, ipsilateral tone burst evoked mVEMPs were obtained from 30 healthy individuals using the zygomatic montage at 500 Hz tone bursts. Self-monitoring biofeedback was given during the procedure to confirm the tension of the masseter muscle. mVEMPs were recorded across three different plateau duration from 0 to 2 ms with rise/fall time of 2 ms at 95dBnHL. Hence the median and interquartile range were calculated for descriptive analysis of the data followed by non-parametric inferential statistics. Results Tone burst evoked mVEMPs were found to be 100% present across all three plateau durations for both ears at 95 dBnHL. P11 and N21 latencies increased with longer plateau durations in both ears. There were no significant differences in P11-N21 peak-to-peak amplitude or interaural amplitude ratio (IAAR) observed across different plateau durations. IAAR was lowest for a 2 ms plateau duration in the 2–2-2 cycle. No significant variations were found across the three plateau durations. Conclusion Significant differences in P11 and N21 latencies were observed across the three stimulus cycles, while no significant differences were found for P11N21 amplitude and IAAR. Yet, there was found to have the highest amplitude and lowest IAAR for 2–2-2 cycle than 2–0-2 and 2–1-2, suggesting it to be the optimal stimulus among the three of them.

Background: Of the 43 mpox cases reported by the WHO in South East Asia between January 2022 and March 2023, 24 (56%) were from India. Objectives: We describe the clinical and epidemiological profile of cases identified through India’s hospital case-based surveillance. Materials and Methods: We identified mpox cases as a positive result for mpox virus polymerase-chain-reaction assay, reported through surveillance from July 1, 2022 to January 7, 2023. Cases and clinicians were interviewed, and data were abstracted from the medical records. We conducted contact tracing among family, close social networks, and healthcare personnel staff for the first 17 cases. We collected the data on sociodemographics, clinical findings, and behavior, and described data using summary statistics. Results: We identified 24 laboratory-confirmed cases (42% females, median age 30 years, range 22–38), including one death (case fatality rate 4.2%). We collected clinical and behavioural data from 21 of 24 cases. All had rashes with vesicles and genital lesions; 7 (33%) reported genital lesions as the first symptom; and 3 (13%) reported complications. Among the 21 cases, all were sexually active, none self-identified as men having sex with men (MSM), and 6 (29%) reported multiple sex partners. We identified 51 contacts of the first 17 reported cases, none reported symptoms suggestive of mpox. Conclusion: The clinical and behavioral characteristics of mpox cases in India are consistent with the global 2022 outbreak, with the exception that no cases in India reported MSM. Most were sexually active young adult economic migrants and developed genital lesions.

Purpose – This paper analyzes the ethics of integrating Artificial Intelligence (AI), particularly regarding AI-generated educational content in academia. It attempts to explore how AI customization mimics human interaction and behavior in education, investigate ethical concerns in educational AI adoption, and assess ChatGPT’s ethical use for nurturing curiosity and maintaining academic integrity in education. Design/methodology/approach – Fictional tales may help us think critically and creatively to uncover hidden truths. The narratives are analyzed to determine the affordances and drawbacks of Artificial Intelligence in Education (AIEd). Findings – The study highlights the imperative for innovative, ethically grounded strategies in harnessing AI/GPT technology for education. AI can enhance learning, and human educators’ irreplaceable role is even more prominent, emphasizing the need to harmonize technology with pedagogical principles. However, ensuring the ethical integration of AI/GPT technology demands a delicate balance where the potential benefits of technology should not eclipse the essential role of human educators in the learning process. Originality/value – This paper presents futuristic academic scenarios to explore critical dimensions and their impact on 21st century learning. As AI assumes tasks once exclusive to human educators, it is essential to redefine the roles of both technology and human teachers, focusing on the future.

Abstract Direct ethanol fuel cells (DEFCs) have been widely considered as a feasible power conversion technology for portable and mobile applications. The economic feasibility of DEFCs relies on two conditions: a notable reduction in the expensive nature of precious metal electrocatalysts and a simultaneous remarkable improvement in the anode's long-term performance. Despite the considerable progress achieved in recent decades in Pt nanoengineering to reduce its loading in catalyst ink with enhanced mass activity, attempts to tackle these problems have yet to be successful. During the ethanol oxidation reaction (EOR) at the anode surface, Pt electrocatalysts lose their electrocatalytic activity rapidly due to poisoning by surface-adsorbed reaction intermediates like CO. This phenomenon leads to a significant loss in electrocatalytic performance within a relatively short time. This review provides an overview of the mechanistic approaches during the EOR of noble metal-based anode materials. Additionally, we emphasized the significance of many essential factors that govern the EOR activity of the electrode surface. Furthermore, we provided a comprehensive examination of the challenges and potential advancements in electrocatalytic EOR. Graphic abstract

Abstract The direct methanol fuel cell (DMFC) represents a highly promising alternative power source for small electronics and automobiles due to its low operating temperatures, high efficiency, and energy density. The methanol oxidation process (MOR) constitutes a fundamental chemical reaction occurring at the positive electrode of a DMFC. Pt-based materials serve as widely utilized MOR electrocatalysts in DMFCs. Nevertheless, various challenges, such as sluggish reaction rates, high production costs primarily attributed to the expensive Pt-based catalyst, and the adverse effects of CO poisoning on the Pt catalysts, hinder the commercialization of DMFCs. Consequently, endeavors to identify an alternative catalyst to Pt-based catalysts that mitigate these drawbacks represent a critical focal point of DMFC research. In pursuit of this objective, researchers have developed diverse classes of MOR electrocatalysts, encompassing those derived from noble and non-noble metals. This review paper delves into the fundamental concept of MOR and its operational mechanisms, as well as the latest advancements in electrocatalysts derived from noble and non-noble metals, such as single-atom and molecule catalysts. Moreover, a comprehensive analysis of the constraints and prospects of MOR electrocatalysts, encompassing those based on noble metals and those based on non-noble metals, has been undertaken.

This study delves into the analysis of recession characteristics of spring base flow, focusing on the Hill campus spring (elevation of approximately 2,150 m) and Fakua spring (elevation of approximately 1,850 m) in the Tehri Garhwal district of Uttarakhand, India. Spanning from January 1999 to December 2004, discharge data from G.B. Pant University of Agriculture and Technology's Hill campus are employed. The research employs an automated, objective-based method to generate master recession curves (MRCs) and categorize them into early, intermittent, and late recession segments. Statistical parameters and low-flow indices from flow duration curves (FDCs) are utilized for flow assessment, while base flow indices (BFIs) are analyzed using the Web-based Hydrograph Analysis Tool (WHAT). The findings highlight the importance of water storage strategies during the rainy season for the sustainable utilization of spring water. The study suggests that the techniques applied are equally applicable for analyzing river flow recession characteristics, emphasizing the broader implications for integrated water resources planning and management in mountainous regions. HIGHLIGHTS This study presents a novel automated and objective-driven method for generating master recession curves (MRCs).; It categorizes them into early, intermittent, and late recession segments.; This method employs statistical parameters and low-flow indices derived from flow duration curves (FDCs).;

According to Chinese Medicine system, Chinese honeysuckle have a lot of valuable metabolites which have a various potential like anti-inflammatory action, antidiabetic, analgesics, antipyretic, eliminates pollutants etc. According to Chinese Medicinal System, the plant is mentioned to cure various diseases like peptic ulcer, diabetes, inflammation etc. It is frequently given for patients with colds that include fever, headaches, and sore throats, but it may also be used to help people who are overheated or under stress chill down. Total 72 articles were found out by the help of online database including Google Scholar, Scopus, PubMed, and Web of Science and 63 had selected. Different keywords like Chinese honeysuckle, Qusqualis indica phytochemistry, pharmacology and toxicology were used to searched the content. The purpose of this review is to summarize the previously reported phytochemicals, pharmacological status of the chosen Chinese plant species. Our findings indicate that Chinese honeysuckle contains a diverse array of bioactive compounds, including flavonoids and terpenoids. Moreover, previous studies have demonstrated that extracts and isolated constituents exhibit significant pharmacological activities and other therapeutic properties. Chinese honeysuckle is a rich source of bioactive compounds that can be incorporated into human diets in low doses and hold potential for treating a range of medical conditions, from minor to severe. This review aims to encourage further research on this plant species, particularly focusing on its toxicity and bioactivity.

Developing novel transition metal complexes with unique structures and properties has garnered significant attention in coordination chemistry. In this work, we prepared two Fe-macrocyclic complexes, A and B, with similar macrocyclic core but differing in the number of phenyl group substitutions via the template approach. The complexes were characterized using several analytical tools. The data were found to be in good agreement with the proposed structure of these complexes. Further electrochemical studies indicated a slight anodic shift in Fe(II) reduction, which can be attributed to the phenyl group effect. The theoretical investigations demonstrated that phenyl groups on the macrocyclic core of complex A significantly reduced the charge density on the Fe center due to extended conjugation, as compared to complex B, making the conversion of Fe(II) to Fe(I) easy. Additionally, the prepared complexes were studied for their antimicrobial activity against various pathogens, and it was found that complex A is more effective against E. coli compared to the standard drug Gentamycin.

Objectives: Wheat despite being an important staple food across the world has been linked to celiac disease in genetically susceptible individuals predominantly caused by gliadins. In this investigation, varietal differences with respect to celiac antigenicity and their relationship with gliadin content were identified. Methods: In this study, we analyzed 62 Indian wheat varieties released over six decades (1961–2020) for gliadin content using BSA and gliadin as calibration standards, and assessed their antigenicity using celiac disease-specific polyclonal antibody (pAb). Results: In addition, a set of 25 wheat varieties was scanned using monoclonal antibody (mAb) raised against gliadin. Large variations in gliadin content (2.75 to 6.98 g 100-1 g whole wheat flour) were observed with the lowest in PBW 34 and the highest in HS 420. Comparative analysis revealed that gliadin content measured using the standard gliadin calibrant was 1.5 times higher than using BSA as calibrant. A positive correlation was observed between total antigenic gliadin and total gliadin content using pAb (r2 = 0.5841; r = 0.763**) and mAb (r2 = 0.4923; r = 0.728**) indicating that total gliadin content is the major factor for eliciting celiac antigenicity. Conclusion: The study also exhibited that celiac causing potential of wheat is not different among the varieties developed across different periods. The findings contribute to our understanding of the relationship between gliadin content and antigenicity, and thus have potential implications in management of celiac disease.

Background and Objective Tobacco smoking is an established risk factor for chronic obstructive pulmonary disease (COPD). Current evidence suggests that non-tobacco-related risk factors vary geographically and are less understood than smoking. This study aims to compare the risk factors, symptoms, and clinical features of smoking (S-COPD) and non-smoking (NS-COPD) in a COPD population. Materials and Methods In this retrospective cross-sectional study, 489 COPD patients were screened. Data on socio-demographics, smoking and medical history, other risk factors, symptoms, and clinical characteristics including COPD Assessment Test (CAT) score, and Modified Medical Research Council (mMRC) Dyspnea Scale were examined. Results Of the total selected 416 COPD patients, 35.34% were NS-COPD while 64.66% were S-COPD. S-COPD was predominant in males, whereas NS-COPD was predominant in females (P < 0.0001). In NS-COPD, biomass fuel exposure was a major risk factor (P < 0.0001), and 61% of subjects had a biomass fuel exposure index of >60. In bivariate and multivariate analyses, no risk factors were correlated with forced expiratory volume in 1 second (FEV1)% predicted, while among clinical features, duration of illness (P = 0.001) was correlated with lower values of FEV1 in the multivariate table of S-COPD. In the multivariate analysis, biomass fuel exposure (P = 0.039) and CAT score (P < 0.0001) were correlated with FEV1(%) in NS-COPD. Conclusion Biomass fuel exposure is a substantial risk factor for NS-COPD and was correlated with FEV1(%) predicted. In addition, the CAT score correlated with disease severity in patients with NS-COPD. The development of COPD in non-smokers is being recognized as a separate phenotype and it should be managed according to risk factors.

Abstract Maternal mortality ratio (MMR) estimates have been studied over time for understanding its variation across the country. However, it is never sufficient without accounting for presence of variability across in terms of space, time, maternal and system level factors. The study endeavours to estimate and quantify the effect of exposures encompassing all maternal health indicators and system level indicators along with space–time effects influencing MMR in India. Using the most recent level of possible -factors of MMR, maternal health indicators from the National Family Health Survey (NFHS: 2019–21) and system level indicators from government reports a heatmap compared the relative performance of all 19 SRS states. Facet plots with a regression line was utilised for studying patterns of MMR for different states in one frame. Using Bayesian Spatio-temporal random effects, evidence for different MMR patterns and quantification of spatial risks among individual states was produced using estimates of MMR from SRS reports (2014–2020). India has witnessed a decline in MMR, and for the majority of the states, this drop is linear. Few states exhibit cyclical trend such as increasing trends for Haryana and West Bengal which was evident from the two analytical models i.e., facet plots and Bayesian spatio- temporal model. Period of major transition in MMR levels which was common to all states is identified as 2009–2013. Bihar and Assam have estimated posterior probabilities for spatial risk that are relatively greater than other SRS states and are classified as hot spots. More than the individual level factors, health system factors account for a greater reduction in MMR. For more robust findings district level reliable estimates are required. As evident from our study the two most strong health system influencers for reducing MMR in India are Institutional delivery and Skilled birth attendance.

Abstract In bread wheat, a literature search gave 228 QTLs for six traits, including resistance against spot blotch and the following five other related traits: (i) stay green; (ii) flag leaf senescence; (iii) green leaf area duration; (iv) green leaf area of the main stem; and (v) black point resistance. These QTLs were used for metaQTL (MQTL) analysis. For this purpose, a consensus map with 72,788 markers was prepared; 69 of the above 228 QTLs, which were suitable for MQTL analysis, were projected on the consensus map. This exercise resulted in the identification of 16 meta-QTLs (MQTLs) located on 11 chromosomes, with the PVE ranging from 5.4% (MQTL7) to 21.8% (MQTL5), and the confidence intervals ranging from 1.5 to 20.7 cM (except five MQTLs with a range of 36.1–57.8 cM). The number of QTLs associated with individual MQTLs ranged from a maximum of 17 in MQTL3 to 8 each in MQTL5 and MQTL8 and 5 each in MQTL7 and MQTL14. The 16 MQTLs, included 12 multi-trait MQTLs; one of the MQTL also overlapped a genomic region carrying the major spot blotch resistance gene Sb1. Of the total 16 MQTLs, 12 MQTLs were also validated through marker-trait associations that were available from earlier genome-wide association studies. The genomic regions associated with MQTLs were also used for the identification of candidate genes (CGs) and led to the identification of 516 CGs encoding 508 proteins; 411 of these proteins are known to be associated with resistance against several biotic stresses. In silico expression analysis of CGs using transcriptome data allowed the identification of 71 differentially expressed CGs, which were examined for further possible studies. The findings of the present study should facilitate fine-mapping and cloning of genes, enabling Marker Assisted Selection.

The integration of multiple technical, economic, environmental, and social criteria establishes Multi-Criteria Decision Analysis (MCDA) as a dependable decision-making tool in the context of interdisciplinary research. This study employs a literature-based methodology to illustrate how MCDA, particularly utilizing the Analytical Hierarchy Process (AHP) and TOPSIS models, has been utilized to tackle intricate decision-making issues. It also highlights the noteworthy discoveries derived from real-world applications, drawing upon previous research and case studies. This study explores the methodologies employed in the commonly utilized AHP and TOPSIS approaches, highlighting their broad applicability across various industries from 2000 to 2023. Additionally, a comprehensive examination of the applications of MCDA has been organized into five distinct sectors, namely supply chain, healthcare, business, resource management, and engineering & manufacturing.

Optoelectronic devices such as solar panels, cameras, screens, and sensors frequently suffer reduced performance due to dust accumulation. Incorporating self-cleaning features not only sustains their efficiency but also minimizes maintenance expenses, especially for large or challenging-to-access setups. In this study, copper doped-zinc oxide (CZO) thin films with varying copper (Cu) concentrations were created using the reactive co-sputtering process. These films underwent a comprehensive analysis, including X-ray diffraction, scanning electron microscopy, and energy-dispersive X-ray spectroscopy to examine their structure, morphology, and elemental composition. Optical properties were investigated through UV–Vis spectroscopy, while hydrophobicity and electrical characteristics were assessed with contact angle and I–V measurements. Spectroscopic ellipsometry was employed to analyse surface attributes. The results confirmed that as Cu concentrations increased, the crystallite and grain size of ZnO in the films also increased. A red shift in the band gap was observed in UV–Vis spectroscopy, and Cu doping effectively reduced the electrical resistivity of the ZnO thin film. The CZO thin film, with a 60 nm thickness, low roughness (1.49 nm), high optical transmittance (around 80.20 %), a wide band gap (3.22 eV), a substantial contact angle (113°), and low electrical resistivity (5.9 × 105 Ω cm), holds great promise for use in transparent, conductive, and self-cleaning optoelectronic devices.

Double perovskites have garnered significant attention for use in renewable energy applications, particularly solar cells. The development of organic-inorganic hybrid halide perovskite solar cells has advanced rapidly over the last ten years. In this article, fundamental aspects of transport properties of Cs2AgGaCl6 have been illustrated using DFT-based simulation. Constantly is covered that the calculated lattice constant agreed with the available experimental data. With an indirect band gap, the electronic profile demonstrates a semiconducting nature. Reporting bandgap of 1.20 eV for Cs2AgGaCl6. This bandgap remains suitable for solar cell semiconductors. The computed values of effective masses reveal that effective masses of holes are greater than those of electrons. Further various optical parameters have also been examined. The absorption of light in the visible and ultraviolet spectrums is guaranteed by the absorption bands 2.7–4.2 eV and 3.5–4.5 eV. We believe that such insights offer a new starting point and a framework for future research. So, knowledge and drive gained from these studies will stimulate more theoretical investigation into novel perovskite-based materials with novel applications.

IntroductionBluetongue (BT) poses a significant threat to the livestock industry, affecting various animal species and resulting in substantial economic losses. The existence of numerous BT virus (BTV) serotypes has hindered control efforts, highlighting the need for broad-spectrum vaccines.MethodologyIn this study, we evaluated the conserved amino acid sequences within key non-structural (NS) proteins of BTV and identified numerous highly conserved murine- and bovine-specific MHC class I-restricted (MHC-I) CD8+ and MHC-II-restricted CD4+ epitopes. We then screened these conserved epitopes for antigenicity, allergenicity, toxicity, and solubility. Using these epitopes, we developed in silico-based broad-spectrum multiepitope vaccines with Toll-like receptor (TLR-4) agonists. The predicted proinflammatory cytokine response was assessed in silico using the C-IMMSIM server. Structural modeling and refinement were achieved using Robetta and GalaxyWEB servers. Finally, we assessed the stability of the docking complexes through extensive 100-nanosecond molecular dynamics simulations before considering the vaccines for codon optimization and in silico cloning.ResultsWe found many epitopes that meet these criteria within NS1 and NS2 proteins and developed in silico broad-spectrum vaccines. The immune simulation studies revealed that these vaccines induce high levels of IFN-γ and IL-2 in the vaccinated groups. Protein-protein docking analysis demonstrated promising epitopes with strong binding affinities to TLR-4. The docked complexes were stable, with minimal Root Mean Square Deviation and Root Mean Square Fluctuation values. Finally, the in silico-cloned plasmids have high % of GC content with > 0.8 codon adaptation index, suggesting they are suitable for expressing the protein vaccines in prokaryotic system.DiscussionThese next-generation vaccine designs are promising and warrant further investigation in wet lab experiments to assess their immunogenicity, safety, and efficacy for practical application in livestock. Our findings offer a robust framework for developing a comprehensive, broad-spectrum vaccine, potentially revolutionizing BT control and prevention strategies in the livestock industry.

Background: Poxviruses comprise a group of large double-stranded DNA viruses and are known to cause diseases in humans, livestock animals, and other animal species. The Mpox virus (MPXV; formerly Monkeypox), variola virus (VARV), and volepox virus (VPXV) are among the prevalent poxviruses of the Orthopoxviridae genera. The ongoing Mpox infectious disease pandemic caused by the Mpox virus has had a major impact on public health across the globe. To date, only limited repurposed antivirals and vaccines are available for the effective treatment of Mpox and other poxviruses that cause contagious diseases. Methods: The present study was conducted with the primary goal of formulating multi-epitope vaccines against three evolutionary closed poxviruses i.e., MPXV, VARV, and VPXV using an integrated immunoinformatics and molecular modeling approach. DNA-dependent RNA polymerase (DdRp), a potential vaccine target of poxviruses, has been used to determine immunodominant B and T-cell epitopes followed by interactions analysis with Toll-like receptor 2 at the atomic level. Results: Three multi-epitope vaccine constructs, namely DdRp_MPXV (V1), DdRp_VARV (V2), and DdRp_VPXV (V3) were designed. These vaccine constructs were found to be antigenic, non-allergenic, non-toxic, and soluble with desired physicochemical properties. Protein-protein docking and interaction profiling analysis depicts a strong binding pattern between the targeted immune receptor TLR2 and the structural models of the designed vaccine constructs, and manifested a number of biochemical bonds (hydrogen bonds, salt bridges, and non-bonded contacts). State-of-the-art all-atoms molecular dynamics simulations revealed highly stable interactions of vaccine constructs with TLR2 at the atomic level throughout the simulations on 300 nanoseconds. Additionally, the outcome of the immune simulation analysis suggested that designed vaccines have the potential to induce protective immunity against targeted poxviruses. Conclusions: Taken together, formulated next-generation polyvalent vaccines were found to have good efficacy against closely related poxviruses (MPXV, VARV, and VPXV) as demonstrated by our extensive immunoinformatics and molecular modeling evaluations; however, further experimental investigations are still needed.

Abstract Synthetic polymers have shown a great impact on every aspect of our life and attained an exponential rise in their production and utilization in the past decades due to their durability, flexibility, moldability, and inexpensive nature. However, the use of natural polymers or development of safe and environment-friendly synthetic bio-based polymers is continuously undergoing for a sustainable future owing to the exhaustion of petroleum-based resources or fossil-based materials, disposal and economical concerns, including government guidelines. In this regard, the development of new sustainable polymers or materials will step up and build a genuinely circular economy by decreasing manufacture or utilization of fossil-based materials as limited reserves.

Menthol mint (Mentha arvensis L.) is an aromatic and medicinal plant worldwide cultivated for high-value essential oil. It comes under angiospermic plant and belongs to family Lamiaceae commonly known as Japanese mint. The high-value essential oil is obtained from the stem and leaf of plant by the process of hydro-distillation through which major ingredient L-menthol is obtained that is used in aroma and pharmaceutical industries. This investigation aimed to reduce water requirements which can enhance its productivity for sustaining menthol mint cultivation in India. The present investigation has been undertaken as treatment combination which includes two varieties viz. i) Kosi and CIM-Kranti; three depths of soil moisture viz. i) 3 cm, ii) 6 cm and iii) 9 cm along with three moisture regimes, i.e., i) 20±5%, ii) 40±5%, and 60±5% available soil moisture (ASM) were tested in split-split plot design during 2018 and 2019 (from February to June). Results from the present study revealed that the maximum oil yield was recorded 116.78 kg ha-1in cv. Kosi and 107.23 kg ha-1 in cv. for CIM-Kranti, whereas menthol yield was recorded 89.23 kg ha-1in cv. Kosi and 85.13 kg ha-1in cv. CIM-Kranti under 6 cm depth of irrigation when applied irrigation at 40±5% available soil moisture (ASM) in menthol mint. However, the lowest water requirement was recorded 118 mm in cv. Kosi and 148 mm in cv. CIM-Kranti. The highest water use efficiency was recorded 0.61 in cv. Kosi and 0.51 kg oil ha-1 mm-1 in cv. CIM- Kranti under 3 cm depth of irrigation and when irrigations were applied at 20±5 % ASM. The highest net returns of $ 1140.91 ha-1 and $ 989.70 ha-1 have been recorded in Kosi and CIM-Kranti, respectively were computed at 6 cm depth of irrigation and when irrigations were applied at 40±5% available soil moisture as compared with other treatments. The irrigation depth (6 cm) applied at 40±5% ASM was found to be a perfect combination for obtaining maximum oil yield, water use efficiency, net returns, and benefit-cost ratio.

Abstract Background The urgent demand for innovative theranostic strategies to combat bacterial resistance to antibiotics is evident, with substantial implications for global health. Rapid diagnosis of life-threatening infections can expedite treatment, improving patient outcomes. Leveraging diagnostic modalities i.e., positron emission tomography (PET) and single photon emission computed tomography (SPECT) for detecting focal infections has yielded promising results. Augmenting the sensitivity of current PET and SPECT tracers could enable effective imaging of pathogenic bacteria, including drug-resistant strains.UBI (29–41), an antimicrobial peptide (AMP) fragment recognizes the S. aureus membrane through electrostatic binding. Radiolabeled UBI (29–41) is a promising SPECT and PET-based tracer for detecting focal infections. 2-APBA (2-acetyl-phenyl-boronic acid), a non-natural amino acid, specifically targets lysyl-phosphatidyl-glycerol (lysyl-PG) on the S. aureus membranes, particularly in AMP-resistant strains. We propose that combining UBI with 2-APBA could enhance the diagnostic potential of radiolabeled UBI. Results Present work aimed to compare the diagnostic potential of two radiolabeled peptides, namely UBI (29–41) and 2-APBA modified UBI (29–41), referred to as UBI and UBI-APBA. APBA modification imparted antibacterial activity to the initially non-bactericidal UBI against S. aureus by inducing a loss of membrane potential. The antibacterial activity demonstrated by UBI-APBA can be ascribed to the synergistic interaction of both UBI and UBI-APBA on the bacterial membrane. To enable PET imaging, we attached the chelator 1,4,7-triazacyclononane 1-glutaric acid 4,7-acetic acid (NODAGA) to the peptides for complexation with the positron emitter Gallium-68 (68Ga). Both NODAGA conjugates were radiolabeled with 68Ga with high radiochemical purity. The resultant 68Ga complexes were stable in phosphate-buffered saline and human serum. Uptake of these complexes was observed in S. aureus but not in mice splenocytes, indicating the selective nature of their interaction. Additionally, the APBA conjugate exhibited superior uptake in S. aureus while preserving the selectivity of the parent peptide. Furthermore, [68Ga]Ga-UBI-APBA demonstrated accumulation at the site of infection in rats, with an improved target-to-non-target ratio, as evidenced by ex-vivo biodistribution and PET imaging. Conclusions Our findings suggest that linking UBI, as well as AMPs in general, with APBA shows promise as a strategy to augment the theranostic potential of these molecules.

In this report, we have investigated the CsMgCl3 compound with the help of the WIEN2K software package. The structural and electronic properties are performed using the full potential augmented plane wave (FP-LAPW) method with the generalised gradient approximation (GGA) approximation as exchange correlation potentials. We used the Birch-Murnaghan equation (BME) to find the structural properties of the material. These include the lattice parameter, the bulk modulus, the first derivative of the bulk modulus, the minimum energy, and the volume. The structural properties match up with the experimental data. Electronic properties in terms of the band structure (BS) and total and partial density of state (T-DOS and P-DOS) profiles of CsMgCl3 using GGA potentials exhibit an indirect wide energy band gap of 5.35 eV. All these properties show that the CsMgCl3 compound is used as a perovskite in solar cells.

Abstract Background Birth preparedness and complication readiness (BPCR) is an essential component of safe motherhood programs. This study aims to systematically identify and synthesize available evidence on birth preparedness and complication readiness among pregnant and recently delivered women in India. Methods The study followed PRISMA guidelines and used databases such as PubMed, Cochrane Library, and ProQuest. Joanna Briggs Institute [JBI] Tool was used for critical appraisal of studies. The meta-analysis was conducted using Comprehensive Meta-Analysis [CMA] tool and R studio software. Statistical heterogeneity was evaluated using visual inspection of the forest plot, Cochran’s Q test, and the I2 statistic results. Funnel plot and Egger’s tests were applied to explore the possibility of the publication bias in the studies [PROSPERO: CRD42023396109]. Result Thirty-five cross-sectional studies reported knowledge on one or more components of birth preparedness [BP], whilst knowledge on complication readiness [CR] or danger signs was reported in 34 included studies. Utilizing the random effect model, the pooled result showed that only about half of the women [49%; 95% CI: 44%, 53%] were aware on BPCR components. This result ranged between 15% [95% CI: 12%, 19%] to 79% [95% CI: 72%, 84%] in Maharashtra and Karnataka respectively [I2 = 94%, p = 90%] is observed across all components [p

This paper reviews optimization models in the context of water resources management and security. The article is instituted on four fundamental pillars: (a) an understanding of the quantum of key optimization techniques adopted by the researchers over the past few decades in managing water resources, (b) an enumeration of these techniques, both in terms of their brief mathematical structures and with reference to their representative applications in managing water resources so as to conform to one of the four perspectives of water security, viz. welfare, equity, sustainability, and risk, (c) an evaluation of major challenges associated with these conventional equation-based optimization techniques, including the perceptive account of the distinction between the gradient-based local optimization and non-gradient global optimization, and finally, (d) an assessment of context-sensitive appropriateness of simulation-based bottom-up modeling schemes, with special reference to evolutionary algorithms. The review emphasizes that the ontology of conventional equation-based models lies in an aggregate manifestation of social behavior and, as a result, it fails to capture individuals’ behaviors juxtaposed with ecological and hydrological systems while modeling complex water resources. On the contrary, the expediency of the domain of operational research in responding to societal problems ensuing from a scarce natural resource like water lies in bottom-up optimization schemes, which are more obliging in the sense that they can incarcerate such social explanations in the modeling frame based on local values.

Abstract Oral iron therapy is often the most common way of treating anaemia; however intravenous iron is considered effective due to rapid iron replenishment. We have dearth of evidence on clinical outcomes post treatment of anaemia. We have searched studies published in English in PubMed, Cochrane, Scopus, ProQuest, and Google Scholar. Our study analysed the clinical outcomes amongst neonates and mother and the adverse events post treatment and assessed the mean change in maternal haemoglobin concentration in both the groups. Forest plots for the clinical outcomes are presented. From a total of 370 studies, 34 Randomized and quasi experimental studies comparing clinical outcomes post-treatment of anaemia in pregnancy were included for quantitative evidence synthesis. Pooled results of maternal clinical outcomes using random effect model [OR: 0.79 (95% CI 0.66; 0.95); 10 outcomes; 17 studies] showed statistically significant difference among both the groups [Moderate quality evidence]; however no significant difference [OR: 0.99 (95% CI 0.86; 1.14); 7 outcomes; 8 studies] have been observed for neonatal complications [Low quality evidence]. The study found that pregnant women receiving IV iron were significantly less likely to experience adverse events as compared with those receiving oral iron [OR 0.39; (95% CI 0.26–0.60)]; 34 studies; 13,909 women; [Low quality evidence]. Findings from meta-regression analysis showed that IV iron is more likely to reduce maternal complications by 21% compared to oral iron. Increase in odds of adverse maternal outcomes was observed due to increase in gestational age and publication year but no effect for the type of drug used. IV iron increases Hb more and at a higher pace than oral iron. Intravenous iron is more likely to avert adverse maternal outcomes and adverse reactions. However, there is no conclusive evidence on its effectiveness on individual maternal outcome or neonatal outcome/s. Protocol registered with PROSPERO CRD42022368346).

Purpose – Tourism has positive and negative sides. Due to ecological disturbance, cultural diffusion, and poor perceptions, tourism can sometimes be seen as a burden to locals and the area. If these perceptions are not addressed appropriately, it can lead to a chain of conflicts. This study explores people's negative perceptions towards tourism in their locality. Methodology/Design/Approach – Data was collected using a structured questionnaire. Respondents were asked to rate their views on a 5-point Likert scale. Both primary and secondary data were used to achieve the objective of the study. Findings – The results indicate that local people are happy to receive tourists in their villages. However, they have certain concerns that need to be addressed for sustainable tourism growth. Anti-social activities such as drugs, alcoholism, prostitution and lack of measures to protect the ethnic group from outside influences are some of the key areas that require immediate attention. Originality of the research – Similar studies have been conducted before, but in the study area (Arunachal Pradesh, India), which is ecologically sensitive and geographically remote, a detailed study on negative perceptions alone was not conducted. Various dimensions were also added to understand healthy and relative perceptions.

Purpose – The level of tourism development in any destination is directly related to the cooperation of the local community, and this is especially true in rural areas in many parts of the world. Many governments initiate development programs related to tourism in rural areas to provide new opportunities for local communities. However, most local communities are unaware of these opportunities. This study therefore examined how tourism forums influence awareness and perceptions of tourism development. Design/Methodology – Data were collected from local tourism entrepreneurs using a structured questionnaire. Tourism entrepreneurs in this study include host families, hotels, gift stores, and transportation entrepreneurs from the rural region. Findings – The results indicate that there are significant differences in perceptions of tourism development between those who attend the forum(s) and those who do not. Furthermore, these differences vary between different types of tourism service providers. Perceptions of tourism service providers are influenced by their knowledge of tourism programs and their participation in public forums. Originality – All respondents in this study were first generation entrepreneurs, which in itself makes this study unique. The fact that tourism forum(s) act as a platform to eliminate misunderstandings between authorities and local communities has not been highlighted and distinguished in previous studies, which makes this study an original contribution.

Abstract Seventh pandemic Vibrio choleare O1 El Tor strain is responsible for the on-going pandemic outbreak of cholera globally. This strain evolved from non-pathogenic V. cholerae by acquiring seventh pandemic gene (VC 2346), pandemic Islands (VSP1 and VSP2), pathogenicity islands (VP1 and VP2) and CTX prophage region. The cholera toxin production is mainly attributed to the presence of ctx gene in these strains. However, several variants of this strain emerged as hybrid strains or atypical strains. The present study aimed to assess the aquatic environment of Cochin, India, over a period of 5 years for the emergence of multidrug resistant V. cholerae and its similarity with seventh pandemic strain. The continuous surveillance and monitoring resulted in the isolation of ctx negative, O1 positive V. cholerae isolate (VC6) from coastal water, Cochin, Kerala. The isolate possessed the biotype specific O1 El Tor tcpA gene and lacked other biotype specific ctx, zot, ace and rst genes. Whole genome analysis revealed the isolate belongs to pandemic sequence type (ST) 69 with the possession of pandemic VC2346 gene, pathogenic island VPI1, VPI2, and pandemic island VSP1 and VSP2. The isolate possessed several insertion sequences and the SXT/R391 family related Integrative Conjugative Elements (ICEs). In addition to this, the isolate genome carried virulence genes such as VgrG, mshA, ompT, toxR, ompU, rtxA, als, VasX, makA, and hlyA and antimicrobial resistance genes such as gyrA, dfrA1, strB, parE, sul2, parC, strA, VC1786ICE9-floR, and catB9. Moreover, the phylogenetic analysis suggests that the isolate genome is more closely related to seventh pandemic V. cholerae O1 N16961 strain. This study reports the first incidence of environmental ctx negative seventh pandemic V. choleare O1 El Tor isolate, globally and its presence in the aquatic system likely to induce toxicity in terms of public health point of view. The presence of this isolate in the aquatic environment warns the strict implementation of the epidemiological surveillance on the occurrence of emerging strains and the execution of flagship program for the judicious use of antibiotics in the aquatic ecosystem.

Following the work on non-stationary fractal interpolation (Mathematics 7, 666 (2019)), we study non-stationary or statistically self-similar fractal interpolation on the Sierpiński gasket (SG). This article provides an upper bound of box dimension of the proposed interpolants in certain spaces under suitable assumption on the corresponding Iterated Function System. Along the way, we also prove that the proposed non-stationary fractal interpolation functions have finite energy.

Hepatitis B virus (HBV) infection remains a significant global health challenge, leading to chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma (HCC). Covalently closed circular DNA (cccDNA) and integrated HBV DNA are pivotal in maintaining viral persistence. Recent advances in CRISPR/Cas technology offer innovative strategies to inhibit HBV by directly targeting both cccDNA and integrated HBV DNA or indirectly by degrading HBV RNAs or targeting host proteins. This review provides a comprehensive overview of the latest advancements in using CRISPR/Cas to inhibit HBV, with a special highlight on newer non-double-strand (non-DSB) break approaches. Beyond the canonical use of CRISPR/Cas for target inhibition, we discuss additional applications, including HBV diagnosis and developing models to understand cccDNA biology, highlighting the diverse use of this technology in the HBV field.

An experiment was conducted to evaluate the impact of diet supplementation of enzymes during hot weather conditions on milk production and nutrient utilization in Murrah buffaloes. Twenty four Murrah buffaloes were distributed randomly into four equal groups (T1, T2, T3 and T4) with six buffaloes in each, on the basis of their body weight, lactation length and test day milk yield. Feeding trial was planned into four treatment groups, viz. T1 (Basal diet without enzyme supplementation), T2 (Basal diet with supplementation of Xylanase @800000 IU), T3 (Basal diet with supplementation of Cellulase @240000 IU) and T4 (Basal diet with supplementation of Xylanase @800000 IU and Cellulase@240000 IU). The results revealed that the average milk yield (5.93 L/day) was higher in T4 as compared to other treatment groups, i.e. 5.47, 5.59 and 5.88 L/day in T1, T2 and T3 group, respectively. Statistically the difference in milk yield was significantly higher in cellulase supplemented groups. The milk fat (%) was significantly higher in enzyme supplemented over control group. The results were shown an increment in all other major milk constituents in enzyme supplemented group in comparison to control group, while statistically the difference was found non-significant. The average DM intake (kg/d, kg/100 kg body wt. and g/kg W0.75) remained static in all treatment groups while the CP intake (g/d, g/100 kg BW and g/kg W0.75) was significantly higher in cellulase supplemented buffaloes. The TDN intake (kg/d, kg/100kg BW and g/kg W0.75) was significantly higher in enzyme supplemented groups. Similarly the digestibility coefficient of CF, NDF and ADF were higher in buffaloes of T3 and T4 over control (T1) group. So, it is concluded from the present study that the milk production and milk composition improves in the combine use of xylanase and cellulase enzyme supplemented groups of buffaloes due to enhanced protein and energy intake and fibre digestibility.

This paper is an attempt to assess the impact of climate on wheat yield in the Indo-Gangetic Plain (IGP) region of India by using panel data analysis. Five IGP states namely Punjab, Haryana, Uttar Pradesh, Bihar, and West Bengal have been considered to frame a panel. The study used the data of climatic and non-climatic variables from 1990 to 2022 to achieve the objective of the study. The Im-Pesaran-Shin unit-root test was applied to check the stationarity of data. The results of the panel least square dummy variable model indicated that all the climatic variables had non significant influence. Among non-climatic variables that help increase wheat yield, fertilizer consumption and mechanization in agriculture were found to have a significant positive impact on wheat yield in the IGP region of India.

The release of organic pollutants and dyes into the environment by industries has had profound and harmful effects on both humans and ecosystems. Graphene oxide (GO) and its reduced form have been investigated for their effectiveness in removing pollutant dyes. GO nano-powder was synthesized using an improved version of Hummer's method and subsequently thermally reduced at various temperatures, including 125, 150, 175, and 200 °C, under vacuum conditions. In the X-ray diffraction spectra, an intense (001) diffraction peak was initially observed at 9.136° (2θ) for pristine GO. This peak gradually shifted towards higher angles as the reduction process took place and eventually disappeared when the GO was reduced at 200 °C. The intensity ratio of the D and G bands (ID/IG ratio) for GO nano-powder in the Raman spectra decreased from 0.94 to 0.76 due to the reduction process. The FTIR spectra of GO and reduced graphene oxide (rGO) also illustrated the reduction process. The bandgap of pristine GO significantly decreased from 2.31 to 0.73 eV, as determined by ultraviolet–visible (UV–Vis) diffuse reflectance spectrophotometry during the reduction process. The surface area and pore volume of both pristine GO and rGO-150 were determined using the BET (Brunauer-Emmett-Teller) and BJH (Barrett-Joyner-Halenda) methods. The results indicated an increase in the BET surface area from 6.61 to 7.86 m2/g and a corresponding enhancement in pore volume from 0.118 to 0.128 cc/g after reduction. The adsorption and photocatalytic degradation behavior of pristine GO and reduced graphene oxides (rGOs) were examined using methylene blue dye. The pristine GO demonstrated impressive adsorption capability, effectively removing the dye by 85.78 % within just 15 min and achieving nearly 97 % removal after 4 h. In contrast, the highest photocatalytic degradation of methylene blue, about 47.58 %, was attained for the rGO sample reduced at 150 °C under the illumination of visible light.

This study aimed to develop and characterize the chitosan bionanoconjugates (BNCs) loaded with zinc (Zn) and salicylic acid (SA) and test their efficacy on wheat seed exposed to chilling stress. BNCs developed were spherical (480 ± 6.0 nm), porous, and positively charged (+25.2 ± 2.4 mV) with regulated nutrient release properties. They possessed complexation efficiency of 78.4 and 58.9 % for Zn, and SA respectively. BET analysis further confirmed a surface area of 12.04 m2/g. Release kinetics substantiated the release rates of Zn and SA, as 0.579 and 0.559 % per hour, along with a half-life of 119.7 and 124.0 h, respectively. BNCs positively affected the germination potential of wheat seeds under chilling stress as observed by significantly (p

The current research introduces a novel dual ultra-wideband rectenna that has been specifically devised for the S and C bands. The rectenna configuration being presented demonstrates the potential to efficiently capture energy from 1.5 GHz to 4.65 GHz, and 5.55 GHz–7.85 GHz. It is noteworthy to mention that the bandwidths (BW) corresponding to these frequency ranges are 3.15 GHz and 2.3 GHz, correspondingly. The envisioned rectenna is designed to cater to a selection of five distinct commercial bands. The frequencies under consideration include 1.8 GHz, UTMS 2.1 GHz, 5G 3.5 GHz, 4.5 GHz, and 5.8 GHz, which fall within the mid band range for 5G technology. Notably, the extended S-band and C band are also included within this range, catering specifically to 5G applications. For the purpose of rectification, a solitary HSMS 2850 Schottky diode has been selected. The maximum recorded Conversion Efficiency (CE) achieved at 1.8 GHz, 2.1 GHz, 3.5 GHz, 4.5 GHz, and 5.8 GHz corresponds to CE values of 67 %, 65 %, 77 %, 71 %, and 71 %, respectively. These CE measurements were obtained under varying input power levels (IPL) of −6 dBm, 4 dBm, 4 dBm, 2 dBm, and 9 dBm, respectively. The rectenna under consideration is designed to operate across five distinct frequency bands. Notably, the CE exceeds 65 % across all of these frequency bands. Consequently, the rectenna exhibits a higher output power. As a result, this modification makes it appropriate for supplying energy to low-power electronic equipment.

ABSTRACTMicroorganisms resist fluoride toxicity using fluoride export proteins from one of several different molecular families. Cariogenic species Streptococcus mutans and Candida albicans extrude intracellular fluoride using a CLCF F-/H+ antiporter and FEX fluoride channel, respectively, whereas oral commensal eubacteria, such as Streptococcus gordonii, export fluoride using a Fluc fluoride channel. In this work, we examine how genetic knockout of fluoride export impacts pathogen fitness in single-species and three-species dental biofilm models. For biofilms generated using S. mutans with the genetic knockout of the CLCF transporter, exposure to low fluoride concentrations decreased S. mutans counts, synergistically reduced the populations of C. albicans, increased the relative proportion of oral commensal S. gordonii, and reduced properties associated with biofilm pathogenicity, including acid production and hydroxyapatite dissolution. Biofilms prepared with C. albicans with genetic knockout of the FEX channel also exhibited reduced fitness in the presence of fluoride but to a lesser degree. Imaging studies indicate that S. mutans is highly sensitive to fluoride, with the knockout strain undergoing complete lysis when exposed to low fluoride for a moderate amount of time. Biochemical purification of the S. mutans CLCF transporter and functional reconstitution establishes that the functional protein is a dimer encoded by a single gene. Together, these findings suggest that fluoride export by oral pathogens can be targeted by specific inhibitors to restore biofilm symbiosis in dental biofilms and that S. mutans is especially susceptible to fluoride toxicity.IMPORTANCEDental caries is a globally prevalent condition that occurs when pathogenic species, including Streptococcus mutans and Candida albicans, outcompete beneficial species, such as Streptococcus gordonii, in the dental biofilm. Fluoride is routinely used in oral hygiene to prevent dental caries. Fluoride also has antimicrobial properties, although most microbes possess fluoride exporters to resist its toxicity. This work shows that sensitization of cariogenic species S. mutans and C. albicans to fluoride by genetic knockout of fluoride exporters alters the microbial composition and pathogenic properties of dental biofilms. These results suggest that the development of drugs that inhibit fluoride exporters could potentiate the anticaries effect of fluoride in over-the-counter products like toothpaste and mouth rinses. This is a novel strategy to treat dental caries.

Industrial Internet of Things (IIoT) is systems aim to facilitate human monitoring and the direction of efficient production of goods in industrial settings by linking a wide variety of intelligent devices such as sensors, actuators, and controllers. This is achieved by utilizing Internet of Things (IoT) to diagnose a problem with a specific IIoT part is to employ a basic diagnostic technique that's based on models and data. Physical models, signal patterns, and machine-learning strategies must be adequately built to account for system challenges. Another factor that could lead to an exponential rise in complexity is the ever-increasing interconnections between different electronic hardware. The knowledge-based defect diagnosis methods boost interoperability in the operation. Users don't need to be experts in the field to benefit from the system's high-level thinking and response to their queries. So, in advanced IIoT systems, a knowledge-based fault diagnostic approach is favored over traditional model-based and data-driven diagnosis methods. The goal of this study is to evaluate recent improvements in the design of knowledge-based defect detection in the context of IIoT systems, deductive and inductive reasoning, and many other forms of logical reasoning. IIoT-based systems have revolutionized industrial settings by connecting intelligent devices such as sensors, actuators, and controllers to enable efficient production and human monitoring. In this survey paper, we explore machine learning-based sensor fusion techniques within the realm of Industrial Internet of Things (IIoT), addressing critical challenges in fault detection and diagnosis.

Abstract Tuning the electronic properties of transition metals using pyrophosphate (P2O7) ligand moieties can be a promising approach to improving the electrochemical performance of water electrolyzers and supercapacitors, although such a material’s configuration is rarely exposed. Herein, we grow NiP2O7, CoP2O7, and FeP2O7 nanoparticles on conductive Ni-foam using a hydrothermal procedure. The results indicated that, among all the prepared samples, FeP2O7 exhibited outstanding oxygen evolution reaction and hydrogen evolution reaction with the least overpotential of 220 and 241 mV to draw a current density of 10 mA/cm2. Theoretical studies indicate that the optimal electronic coupling of the Fe site with pyrophosphate enhances the overall electronic properties of FeP2O7, thereby enhancing its electrochemical performance in water splitting. Further investigation of these materials found that NiP2O7 had the highest specific capacitance and remarkable cycle stability due to its high crystallinity as compared to FeP2O7, having a higher percentage composition of Ni on the Ni-foam, which allows more Ni to convert into its oxidation states and come back to its original oxidation state during supercapacitor testing. This work shows how to use pyrophosphate moieties to fabricate non-noble metal-based electrode materials to achieve good performance in electrocatalytic splitting water and supercapacitors.

Aluminium alloys are indispensable in all manufacturing industries, particularly mechanical engineering. The objective of this study is to enhance the mechanical, wear, and corrosion properties of aluminium alloy AA5083 by incorporating nanoparticles as reinforcements, thereby creating hybrid aluminium nanocomposites. The base material utilised in this study was AA5083, with the reinforcement nanoparticles selected as Carbon nanotubes (CNTs) and Molybdenum disulfide (MoS2) at concentrations of 5 % and 3 % respectively. Nanocomposites were fabricated using the Powder Metallurgy (PM) technique, employing specific contrasting parameters including Ball mill speed (280, 320, 360, and 400 rpm), Mixing time (20, 30, 40, and 50 min), Compaction pressure (300, 350, 400, and 450 MPa), and Sintering time (2, 3, 4, and 5 h). The operating parameters of powder metallurgy were assessed using the Design of Experiments (DOE) L16 Orthogonal Array, and their corresponding outcomes were analyzed. The results revealed that there is a significant correlation between the mixing time and microhardness of the nanocomposites. The wear and corrosion reduction rates had a significant impact on the sintering time parameter. The present study determined that the highest recorded microhardness value was 136 VHN, while the lowest observed rates of wear and corrosion were 0.192 mm3/m and 0.00081 mm/year, respectively. Scanning electron microscopy (SEM) images clearly depicted the presence of various structural defects, such as pits, cracks, delamination, voids, and cusps, in the wear and corrosion samples that exhibited reduced strength.

Statistical downscaling (SD) is preferable to dynamic downscaling to derive local-scale climate change information from large-scale datasets. Many statistical downscaling models are available these days, but comparison of their performance is still inadequately addressed for choosing a reliable SD model. Thus, it is desirable to compare the performance of SD models to ensure their adaptability in future climate studies. In this study, a statistical downscaling model (SDSM) or multi-linear regression and the Least Square Support Vector Machine (LS-SVM) were used to do downscaling and compare the results with those obtained from general circulation model (GCM) for identifying the best SD model for the Indira Sagar Canal Command area located in Madhya Pradesh, India. The GCM, Hadley Centre Coupled Model version 3 (HadCM3), was utilized to extract and downscale precipitation, maximum temperature (Tmax), and minimum temperature (Tmin) for 1961–2001 and then for 2001–2099. Before future projections, both SD models were initially calibrated (1961–1990) and validated (1991–2001) to evaluate their performance for precipitation and temperature variables at all gauge stations, namely Barwani, East Nimar, and West Nimar. Results showed that the precipitation trend was under-predicted owing to large errors in downscaling, while temperature was over-predicted by SD models. HIGHLIGHTS Precipitation values are under-predicted, while temperature values are over-predicted by statistical downscaling models.; Large errors in downscaling precipitation are observed, since downscaling of precipitation is more problematic than temperature.; Statistical measures (R2, RMSE, SSE, NSE, and MAE) showed good agreement between observed and downscaled climate variables for SDSM and LS-SVM.;

Abstract The present research shows the application of Taguchi's design of experiment approach to optimize the process parameters for the removal of phenol onto surface of Saccharum officinarum biomass activated carbon (SBAC) from an aqueous solution to maximize adsorption capacity of SBAC. The effect of adsorption parameters viz. adsorbent dose (m), temperature (T), initial concentration (C0) and mixing time (t) on response characteristics i.e., adsorption capacity (qt) has been studied at three levels by using L9 orthogonal array (OA) which further analyzed by variance analysis (ANOVA) for adsorption data and signal/noise (S/N) ratio data by using ‘larger the better’ characteristics. Using ANOVA, the optimum parameters are found to be m = 2 g/L, C0 = 150 mg/L, T = 313 K and t = 90 min, resulting in a maximum adsorption capacity of 64.59 mg/g. Adopting ANOVA, the percentage contribution of each process parameter in descending order of sequence is adsorbent dose 59.97% > initial phenol concentration 31.70% > contact time 4.28% > temperature 4.04%. The phenol adsorption onto SBAC was best fitted with the pseudo-second-order kinetic model and follows the Radke-Prausnitz isotherm model. Thermodynamic parameters suggested a spontaneous, exothermic nature and the adsorption process approaches physisorption followed by chemisorption. Hence the application of Taguchi orthogonal array design is a cost-effective and time-efficient approach for carrying out experiments and optimizing procedures for adsorption of phenol and improve the adsorption capacity of SBAC.

Abstract In a special class of almost contact metric manifolds known as cosymplectic manifolds, the current study aims to establish the existence result and a few inequalities for sequential warped product submanifolds. These results and inequalities represent fruitful connections between the primary intrinsic and extrinsic invariants. Furthermore, findings related to Dirichlet energy have been addressed. Finally, some exceptional cases resulting in several inequities are examined.

New forms of interaction made possible by developments in special educational technologies can now help students with dyscalculia. Artificial intelligence (AI) has emerged as a promising tool in recent decades, particularly between 2001 and 2010, offering avenues to enhance the quality of education for individuals with dyscalculia. Therefore, the implementation of AI becomes crucial in addressing the needs of students with dyscalculia. Content analysis techniques were used to examine the literature covering the influence of AI on dyscalculia and its potential to assist instructors in promoting education for individuals with dyscalculia. The study sought to create a foundation for a more inclusive dyscalculia education in the future through in-depth studies. AI integration has had a big impact on educational institutions as well as people who struggle with dyscalculia. This paper highlights the importance of AI in improving the educational outcomes of students affected by dyscalculia.

Rural transformation is one of the key social and economic changes that lead to the development of rural communities and the overall growth of any country. A country like India has diversity in terms of the availability of natural resources, work culture, race, and religion for each geographical state unit with its own pace of growth and development. Gujarat is one of the high-growth states in the country, with Gross State Domestic Product (GSDP) estimates of USD 253.64 billion in FY22 with a 7% YoY increase (India Brand Equity Foundation, n.d.). However, the rural population in Gujarat fell to 57.4% in 2011 from 62.6% in the 2001 census. This paper tries to assess the rural transformation in Gujarat with multidimensional indices like agriculture, consumption, employment, education, and urbanization that focus on rural transformation with the help of district-level microdata in the broader context of the high economic growth of Gujarat.

Abstract The challenge of endergonic reduction of NADP+ using NADH is overcome by ferredoxin-dependent transhydrogenases that employ electron bifurcation for electron carrier adjustments in the ancient Wood-Ljungdahl pathway. Recently, an electron-bifurcating transhydrogenase with subunit compositions distinct from the well-characterized Nfn-type transhydrogenase was described: the Stn complex. Here, we present the single-particle cryo-EM structure of the Stn family transhydrogenase from the acetogenic bacterium Sporomusa ovata and functionally dissect its electron transfer pathway. Stn forms a tetramer consisting of functional heterotrimeric StnABC complexes. Our findings demonstrate that the StnAB subunits assume the structural and functional role of a bifurcating module, homologous to the HydBC core of the electron-bifurcating HydABC complex. Moreover, StnC contains a NuoG-like domain and a GltD-like NADPH binding domain that resembles the NfnB subunit of the NfnAB complex. However, in contrast to NfnB, StnC lost the ability to bifurcate electrons. Structural comparison allows us to describe how the same fold on one hand evolved bifurcation activity on its own while on the other hand combined with an associated bifurcating module, exemplifying modular evolution in anaerobic metabolism to produce activities critical for survival at the thermodynamic limit of life.

Abstract Although porphyry systems like metallo-phthalocynine are recognized as promising molecular models for electrocatalytic oxygen reduction reaction (ORR), their poor durability and methanol tolerance are still challenges and need improvement before being considered for practical applications. Herein, we successfully designed and constructed a Fe-phthalocyanine-derived highly conjugated 2D covalent organic framework (2D FePc-COF), using octa-amino-Fe-phthalocyanine (OA-FePc) and cyclohexanone as precursors. The prepared 2D FePc-COF was characterized via multiple analytic techniques. The electrochemical studies indicated that prepared 2D FePc-COF was far more superior to OA-FePc and 20% Pt/C, displaying anodic shift of 100 and 50 mV (vs RHE) in formal potential, respectively. Moreover, this catalyst also demonstrated excellent methanol tolerance and durability (over 10,000 CV cycles). Theoretical investigations revealed that due to extended conjugation and elimination of electron donating groups (-NH2), the shifting of dz2-orbital (Fe) energy took nearer to π*-orbital (O2), allowing optimum coupling of both the orbitals, thereby enhancing 4e− ORR. This work demonstrates the art of molecular design, aiming at improving catalytic activity of macrocyclic molecular systems towards ORR.

Chronic hepatitis B virus (HBV) infection remains a global health problem due to the lack of treatments that prevent viral rebound from HBV covalently closed circular (ccc)DNA. In addition, HBV DNA integrates in the human genome, serving as a source of hepatitis B surface antigen (HBsAg) expression, which impairs anti-HBV immune responses. Cytosine base editors (CBEs) enable precise conversion of a cytosine into a thymine within DNA. In this study, CBEs were used to introduce stop codons in HBV genes, HBs and Precore. Transfection with mRNA encoding a CBE and a combination of two guide RNAs led to robust cccDNA editing and sustained reduction of the viral markers in HBV-infected HepG2-NTCP cells and primary human hepatocytes. Furthermore, base editing efficiently reduced HBsAg expression from HBV sequences integrated within the genome of the PLC/PRF/5 and HepG2.2.15 cell lines. Finally, in the HBV minicircle mouse model, using lipid nanoparticulate delivery, we demonstrated antiviral efficacy of the base editing approach with a >3log10 reduction in serum HBV DNA and >2log10 reduction in HBsAg, and 4/5 mice showing HBsAg loss. Combined, these data indicate that base editing can introduce mutations in both cccDNA and integrated HBV DNA, abrogating HBV replication and silencing viral protein expression.