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263 results on '"Anuar, Nurina"'

3. Utilization of Bioreactor Operation Mode in Producing Influenza Vaccine Syariah-Compliant

Author

Ros, F. N. Aliya Mohamad, Rahman, Norliza Abd., Ali, Jarinah Mohd, Anuar, Nurina, Abdullah, Siti Rozaimah Bt. Sheikh, Jusoh, Amir Fazlim Bin, Md Shariff, Nur Nafhatun, editor, Lateh, Najahudin, editor, Zarmani, Nur Farhani, editor, Hamidi, Zety Sharizat, editor, Abdulrazzak Aghwan, Zeiad Amjad, editor, Binti Dato Haji Mahalle, Norliza, editor, Haji Yaacob, Hakimah, editor, and Hisao, Tomizawa, editor

Published
2021
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9. A Comparative Analysis between Direct and Indirect Measurement of Year I Integrated Project

Author

Abdullah, Siti Rozaimah Sheikh, Mohamad, Abu Bakar, Anuar, Nurina, Markom, Masturah, Ismail, Manal, Rosli, Masli Irwan, and Hasan, Hassimi Abu

Abstract

The Integrated Project (IP) has been practised in the Department of Chemical and Process Engineering (JKKP) since the 2006/2007 session. Initially, the IP is only implemented for the Year II students for both Chemical (KK) and Biochemical Engineering (KB) programmes. Previously, the Year 1 curriculum was only based on the common faculty courses. Starting in the 2010/2011 session, a new curriculum was introduced for Year I with some reshuffling of the courses offered, in which the programme syllabuses are tailored to the offered departmental offerings starting in Year I. Based on the new curriculum, the students registered under the KK and KB programmes have undergone the IP since Semester I of their first-year studies. This implementation project encourages and gives the opportunity to students to take part in the open-ended problem-solving project. The students are trained to obtain information independently through lifelong learning, communication skills in terms of written and oral communications and identify the current issues and involve team work. The training can reduce the phenomenon of student dependence on lecturers or "spoon-feeding" to obtain information in order to understand a theory of learning. The exposure could also transform the learning scenario from lecture-centred to student-centred learning, which requires students to participate more actively in the learning process. The student achievement of the programme outcomes (POs), which has been set in terms of lecturer assessment (direct measurement) and student perception through questionnaires (indirect measurement), was compared at the end of the implementation of the IP in Semester II of the 2011/2012 session. From the comparison, it was found that both measurement methods gave very similar results for all the outlined POs with insignificant differences detected between the POs. This proves that the students gain benefits through the implementation of the IP which are in line with the lecturers' expectations.

Published
2013

18. An investigation of the nutrient gradients in aggregates of animal cells in culture

Author

Anuar, Nurina

Subjects
572.8, Monoclonal antibody development
Abstract

The investigations of nutrient gradients in animal cell aggregates were conducted on three types of cell; HeLa S3, genetically engineered BHK and D36 hybridoma cells for the production of poliovirus, alkaline phosphatase enzyme and monoclonal antibody, respectively as their biological products. Agitation speed in stirred flasks controlled the size of HeLa S3 and BHK aggregate formation and had adverse an effect on hybridoma cells by reducing their viability. The three types of cells showed a preference to grow in roller bottles, where cell aggregation was promoted and cell growth was unaffected. Addition of calcium alone up to 750μM into the culture media, which already contained 423μM calcium ions, induced the formation of HeLa S3 cell aggregates without affecting cell growth. However, the cell viability was reduced when calcium was added at higher than 750μM. Relatively larger aggregates and more extensive cell growth were obtained when collagen solution was added in combination with calcium into the culture media. Because of its potential in promoting cell aggregation, a desiccated type of collagen made from calf skin, selected from the commercially available ranges via collagen fast tests, was used in these investigations. Various concentrations with or without calcium were added into the cell culture media to grow the three types of cell. Different types of cell growth were observed, through manipulation of the cell growth parameters accordingly to the aggregate sizes which enabled the indirect determination of nutrient gradients in HeLa S3 and BHK aggregates. Positive gradients in HeLa S3 and BHK aggregates showed that the cell viability and metabolic activity were increased with the enlargement of the aggregates. By contrast, efforts to achieve cell aggregation were unsuccessful with hybridoma cells where calcium and collagen additions gave adverse effects on growth and productivity. Optimum concentrations of both calcium and collagen were sought for HeLa S3 and BHK cells, for inducing the largest aggregate formation and maximum cell growth. Combinations of 750pM calcium and 0. 0025% collagen for HeLa S3 cells and 500μM calcium and 0. 0075% collagen for BHK cells were applied for the formation of the largest aggregates and maximum growth. These aggregates were used for direct determination of nutrient gradient within the aggregates by cell staining and confocal microscopy technique. No cell viability gradient was found within the aggregates due to the loosely packed viable HeLa S3 cells, while the dead cells were released from the intact aggregated cells. Meanwhile, the insertion of an O2 microelectrode into BHK aggregates depicted the pO2 level within the aggregates. The morphology of aggregates determined the pattern of pO2 profiles where spherical shaped aggregates exhibited zero and low pO2 areas towards the centre of aggregates. Addition of calcium and collagen improved cell growth where the low and zero pO2 area was reduced even though the larger aggregates were >1000um in diameter. The formation of larger aggregates from the optimum concentration of calcium and collagen addition also increased the biological production of HeLa S3 and BHK cells. HeLa S3 aggregates produced almost twice the amount of poliovirus titre over the slightly aggregated control cultures. Similarly, larger BHK aggregates produced twice to four times higher alkaline phosphatase enzyme compared with the control untreated BHK aggregate culture.

Published
1998

36. Analysis of Integrated Project Effectiveness in the Implementation of Generic Skills

Author

Abdullah, Siti Rozaimah Sheikh, Takriff, Mohd Sobri, Ismail, Manal, Kalil, Mohd Sahaid, Daud, Wan Ramli Wan, Mohammad, Abu Bakar, Rahman, Rakmi Abd., Mohammad, Abdul Wahab, Anuar, Nurina, Abdullah, Normah, Harun, Shuhaida, Kamarudin, Siti Kartom, Tasirin, Siti Masrinda, Yaakob, Zahira, Jahim, Jamaliah, Kofli, Noor Hisham Tan, Markom, Masturah, Rahman, Norliza Abd., Rahim, Rosiah Rohani Reehan Adne Ab., and Rahaman, Muhammad Syukri Abd.

Published
2012
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