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This study is focused on the hyperbolic partial differential equations (HPDEs) to obtain their numerical solution. Here, the Vieta–Lucas Tau operational approach (VLTM) is proposed and utilized to solve the initial value problems related to HPDEs. In this approach, shifted Vieta–Lucas polynomials (SVLPs) are employed as the basis functions to assume the general solution. The integral and derivative operational matrices for SVLPs are built over a generalized domain. These operational matrices are used to convert this problem into a collection of algebraic equations. The examination of the convergence errors is well covered. The projected accuracy and efficacy of the method are confirmed when several cases are taken into account. Excellent agreement between exact and estimated findings is obtained, and in certain situations, estimated values are remarkably accurate. Finally, we can state that the VLTM method is dependable, efficient, precise, and easy to use.

Salmonella enterica is a foodborne pathogen associated with both typhoid and non-typhoid illness in humans and animals. This problem is further exacerbated by the emergence of antibiotic-resistant strains of Salmonella enterica. Therefore, to meet public health and safety, there is a need for an alternative strategy to tackle antibiotic-resistant bacteria. Bacteriophages or (bacterial viruses), due to their specificity, self-dosing, and antibiofilm activity, serve as a better approach to fighting against drug-resistant bacteria. In the current study, a broad-host range lytic phage phiSalP219 was isolated against multidrug-resistant Salmonella enterica serotypes Paratyphi from a pond water sample. Salmonella phage phiSalP219 was able to lyse 28/30 tested strains of Salmonella enterica. Salmonella phage phiSalP219 exhibits activity in acidic environments (pH3) and high temperatures (70°C). Electron microscopy and genome analysis revealed that phage phiSalP219 is a member of class Caudoviricetes. The genome of Salmonella phage phiSalP219 is 146Kb in size with 44.5% GC content. A total of 250 Coding Sequence (CDS) and 25 tRNAs were predicted in its genome. Predicted open reading frames (ORFs) were divided into five groups based on their annotation results: (1) nucleotide metabolism, (2) DNA replication and transcription, (3) structural proteins, (4) lysis protein, and (5) other proteins. The absence of lysogeny-related genes in their genome indicates that Salmonella phage phiSalP219 is lytic in nature. Phage phiSalP219 was also found to be microbiologically safe (due to the absence of toxin or virulence-related genes) in the control of Salmonella enterica serovar Typhimurium infections in the ready-to-eat meat and also able to eradicate biofilm formed by the same bacterium on the borosilicate glass surface.

In the present scenario, the challenge of emerging antimicrobial resistance is affecting human health globally. The increasing incidences of multidrug-resistant infections have become harder to treat, causing high morbidity, and mortality, and are posing extensive financial loss. Limited discovery of new antibiotic molecules has further complicated the situation and has forced researchers to think and explore alternatives to antibiotics. This has led to the resurgence of the bacteriophages as an effective alternative as they have a proven history in the Eastern world where lytic bacteriophages have been used since their first implementation over a century ago. To help researchers and clinicians towards strengthening bacteriophages as a more effective, safe, and economical therapeutic alternative, the present review provides an elaborate narrative about the important aspects of bacteriophages. It abridges the prerequisite essential requirements of phage therapy, the role of phage biobank, and the details of immune responses reported while using bacteriophages in the clinical trials/compassionate grounds by examining the up-to-date case reports and their effects on the human gut microbiome. This review also discusses the potential of bacteriophages as a biocontrol agent against food-borne diseases in the food industry and aquaculture, in addition to clinical therapy. It finishes with a discussion of the major challenges, as well as phage therapy and phage-mediated biocontrols future prospects.

Determination of the ploidy level of plants is utmost important for identification of species, selection of suitable parental lines and also to identify the relationships between species to determine the evolutionary patterns. In chrysanthemum species, chromosomes often have different degrees of differentiation, both between and within species. Total 30 genotypes of Chrysanthemum (Chrysanthemum morifolium Ramat.) were used to study the cytology in the Department of Floriculture and Landscaping and Department of Plant Breeding and Genetics, PAU, Ludhiana during 2019 with the objective to find out the genetic variation among the diverse genotypes of chrysanthemum. The ploidy level of 30 chrysanthemum genotypes was studied through meiotic study of pollen mother cells and for this study of meiotic chromosomes, stage and timings of bud collection is very much important. A range of ploidy level was encountered in chromosomal study of selected chrysanthemum germplasm and ploidy level was 2n= 52–116. This study indicated that polyploidy variation was found in all varieties of chrysanthemum selected for study. The elucidation of ploidy level of various crops and species provides foundation for understanding their genetic background, therefore, the results of present study will be further helpful for germplasm conservation of chrysanthemum flower crop.

Purpose Solid waste management has become a serious global problem. There is a strong need to recycle them as these wastes are rich in plant nutrients and soil conditioners. The different organic wastes can be efficiently degraded into nutrient-rich vermicompost by using earthworms. In this review, an attempt has been made to highlight the vermicomposting of different organic wastes by using different earthworm species. Methods An extensive literature search was done on Science Direct, Pubmed Central, Google Scholar, Springerlink by using various search strings, and appropriate studies of vermicomposting of different organic waste were selected. Results Any kind of organic waste can be converted into manure through vermicomposting. It was observed that for efficient vermicomposting, the waste should be mixed with another organic material. e.g., animal dung. It was observed that the vermireactors having 25 % to 30 % of waste mixed with about 70 % to 75 % of other organic rich material like cattle dung can be easily converted into a valuable product, but the high proportion of organic waste causes mortality in the earthworms. Conclusion This study indicated that vermicomposting is an effective and a better option for the recycling of different types of organic solid waste but these wastes cannot be directly degraded with the help of earthworms. The vermicompost so produced can be used to promote the growth of wide range of crops in the fields. The farmers should also be educated regarding the harmful effects of chemical fertilizers and pesticides and also get motivated to use vermicompost in their fields.

Extensive and multiple drug resistance in P. aeruginosa combined with the formation of biofilms is responsible for its high persistence in nosocomial infections. A sequential method to devise a suitable phage cocktail with a broad host range and high lytic efficiency against a biofilm forming XDR P. aeruginosa strain is presented here. Out of a total thirteen phages isolated against P. aeruginosa, five were selected on the basis of their high lytic spectra assessed using spot assay and productivity by efficiency of plating assay. Phages, after selection, were tested individually and in combinations of two-, three-, four-, and five-phage cocktails using liquid infection model. Out of total 22 combinations tested, the cocktail comprising four phages viz. φPA170, φPA172, φPA177, and φPA180 significantly inhibited the bacterial growth in liquid infection model (p < 0.0001). The minimal inhibitory dose of each phage in a cocktail was effectively reduced to >10 times than the individual dose in the inhibition of XDR P. aeruginosa host. Field emission-scanning electron microscopy was used to visualize phage cocktail mediated eradication of 4-day-old multi-layers of XDR P. aeruginosa biofilms from urinary catheters and glass cover slips, and was confirmed by absence of any viable cells. Differential bacterial inhibition was observed with different phage combinations where multiple phages were found to enhance the cocktail’s lytic range, but the addition of too many phages reduced the overall inhibition. This study elaborates an effective and sequential method for the preparation of a phage cocktail and evaluates its antimicrobial potential against biofilm forming XDR strains of P. aeruginosa.

In this paper, a novel feature descriptor, local texton XOR patterns (LTxXORP) is proposed for content-based image retrieval. The proposed method collects the texton XOR pattern which gives the structure of the query image or database image. First, the RGB (red, green, blue) color image is converted into HSV (hue, saturation and value) color space. Second, the V color space is divided into overlapping subblocks of size 2 × 2 and textons are collected based on the shape of the textons. Then, exclusive OR (XOR) operation is performed on the texton image between the center pixel and its surrounding neighbors. Finally, the feature vector is constructed based on the LTxXORPs and HSV histograms. The performance of the proposed method is evaluated by testing on benchmark database, Corel-1K, Corel-5K and Corel-10K in terms of precision, recall, average retrieval precision (ARP) and average retrieval rate (ARR). The results after investigation show a significant improvement as compared to the state-of-the-art features for image retrieval.

Functional and sensory properties of cookies prepared by supplementing different proportions of cassava flour (CF) and water chestnut flour (WCF) blends (0–100%) to wheat flour (WF) were studied. Seven formulations of cookies were prepared from (a) Control (100% WF), (b) 30% WF, 35% WCF and 35% CF, (c) 27% WF, 37.5% WCF and 37.5% CF, (d) 20% WF, 40% WCF and 40% CF, (e) 15% WF, 42.5% WCF and 42.5% CF, (f) 10% WF, 45% WCF and 45% CF, and (g) 0% WF, 50% WCF and 50% CF. Cookies were subjected to physical analysis (cookie diameter, cookie thickness, spread ratio, bulk volume, bulk density, breaking strength, and color analysis) and evaluated for consumer acceptance by descriptive sensory analysis. Cookies prepared from water chestnut and cassava flour had low moisture content (5.63%), low fat (24.87%), higher spread ratio (8.148), decreased L, a and b values (dark color), and low breaking strength than control ones. Sensory evaluation established that cookies prepared from 50% WCF and 50% CF were more acceptable than cookies prepared from other formulations.

Salmonella enterica is a foodborne pathogen associated with both typhoid and non-typhoid illness in humans and animals. This problem is further exacerbated by the emergence of antibiotic-resistant strains of Salmonella enterica. Therefore, to meet public health and safety, there is a need for an alternative strategy to tackle antibiotic-resistant bacteria. Bacteriophages or (bacterial viruses), due to their specificity, self-dosing, and antibiofilm activity, serve as a better approach to fighting against drug-resistant bacteria. In the current study, a broad-host range lytic phage phiSalP219 was isolated against multidrugresistant Salmonella enterica serotypes Paratyphi from a pond water sample. Salmonella phage phiSalP219 was able to lyse 28/30 tested strains of Salmonella enterica. Salmonella phage phiSalP219 exhibits activity in acidic environments (pH3) and high temperatures (70°C). Electron microscopy and genome analysis revealed that phage phiSalP219 is a member of class Caudoviricetes. The genome of Salmonella phage phiSalP219 is 146Kb in size with 44.5% GC content. A total of 250 Coding Sequence (CDS) and 25 tRNAs were predicted in its genome. Predicted open reading frames (ORFs) were divided into five groups based on their annotation results: (1) nucleotide metabolism, (2) DNA replication and transcription, (3) structural proteins, (4) lysis protein, and (5) other proteins. The absence of lysogeny-related genes in their genome indicates that Salmonella phage phiSalP219 is lytic in nature. Phage phiSalP219 was also found to be microbiologically safe (due to the absence of toxin or virulence-related genes) in the control of Salmonella enterica serovar Typhimurium infections in the ready-to-eat meat and also able to eradicate biofilm formed by the same bacterium on the borosilicate glass surface. [ABSTRACT FROM AUTHOR]

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