1. Growth and differentiation in vitro of the accumulating Lyt-2-/L3T4- subset in lpr mice
- Author
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R C, Budd, H R, MacDonald, J W, Lowenthal, J L, Davignon, S, Izui, and J C, Cerottini
- Subjects
Immunology ,Receptors, Immunologic/metabolism ,chemical and pharmacologic phenomena ,Cell Cycle/drug effects ,ddc:616.07 ,Lymphocyte Activation ,Mice ,immune system diseases ,Concanavalin A/pharmacology ,Concanavalin A ,Antigens, Ly/genetics ,Antigens, Ly ,Immunology and Allergy ,Animals ,Antigens, Surface/genetics ,Receptors, Immunologic ,Cell Cycle ,Tetradecanoylphorbol Acetate/pharmacology ,Cell Differentiation ,hemic and immune systems ,Receptors, Interleukin-2 ,Lymphocyte Activation/drug effects ,Lymphocyte Function-Associated Antigen-1 ,T-Lymphocytes, Cytotoxic/ classification/cytology/immunology/metabolism ,Mice, Inbred C57BL ,Lymph Nodes/cytology ,Phenotype ,Cell Differentiation/drug effects ,Antigens, Surface ,Tetradecanoylphorbol Acetate ,Lymph Nodes ,Mice, Inbred C57BL/ genetics ,T-Lymphocytes, Cytotoxic - Abstract
Mice bearing the recessive gene lpr develop an autoimmune syndrome associated with a massive lymphadenopathy, both of which are age and thymus dependent. The predominant accumulating cells in lymphoid tissue of lpr/lpr mice are Thy-1+ but express neither of the mature T cell markers, Lyt-2 or L3T4. We have purified this Lyt-2-/L3T4- subset and examined its phenotype. These cells are not actively cycling, do not express interleukin-2 (IL 2) receptors nor significant levels of antigen receptor, but do express the B cell marker B220. In vitro growth conditions were examined for the lpr Lyt-2-/L3T4- subset. By using a combination of phorbol ester and IL 2, these cells acquired transient expression of IL 2 receptors and grew in an IL 2-dependent manner. Furthermore, these proliferating cells underwent differentiation to a more mature T cell phenotype, with loss of cell surface B220 and acquisition, by a portion, of antigen receptor and Lyt-2. The possible parallels with normal T cell maturation are discussed.
- Published
- 1985