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Objective: Lower respiratory tract infections are one of the diseases causing high morbidity and mortality worldwide. The aim of this study was to determine the pathogens isolated from lower respiratory tract specimens and their antibiotic resistance status. Methods: All lower respiratory tract samples sent to our laboratory between 2018 and 2022 were evaluated retrospectively. In addition to conventional methods, species identification and antibiotic susceptibility tests were performed with an automated system (BD Phoenix 100). Results: Over five years, a total of 2889 isolates were obtained from lower respiratory tract samples. Among these, 61,33% were from male patients. Most respiratory pathogens (74,59%) were isolated from intensive care units, followed by inpatient wards (15,75%) and outpatient clinics (9,66%). Of the 2889 strains, 85.12% were identified as gram-negative bacteria, 10.59% as gram-positive bacteria and 4.29% as Candida spp. Predominant microorganisms included Acinetobacter baumannii (32,12%), Pseudomonas aeruginosa (21,32%), Klebsiella pneumoniae (16,68%) and Staphylococcus aureus (9,10%). Acinetobacter baumannii showed high resistance to all tested antibiotics (81,20-97,53%). Pseudomonas aeruginosa exhibited 76,48% resistance to levofloxacin but 88,3% susceptibility to amikacin. Klebsiella pneumoniae displayed high resistance rates to tested antibiotics (55.94-92.33%). Staphylococcus aureus strains were 100% resistant to penicillin, with half showing resistance to oxacillin (49,61%) but high susceptibility to vancomycin (100%), linezolid (99,62%), and teicoplanin (98,86%). Conclusion: This study underscores the significant antimicrobial resistance observed among bacteria isolated from respiratory tract samples. Accurate identification of microorganisms causing lower respiratory tract infections and their antibiotic susceptibility patterns is crucial for selecting targeted and effective antibiotic therapy and preventing the emergence of resistant bacteria. [ABSTRACT FROM AUTHOR]

Background: This study aims to compare the colistin susceptibility detected by the BD Phoenix automated system among multidrug-resistant bacteria with the broth microdilution (BMD) method results. Materials and Methods: The study included 236 isolates (92 A. baumannii, 68 K. pneumoniae, 55 P. aeruginosa, and 21 E. coli) isolated from clinical samples submitted to the bacteriology laboratory of Dicle University Hospital. The BD Phoenix 100 automated system was evaluated using BMD as a reference method. Categorical agreement (CA, the number of concordant results), major errors (ME, results falsely categorized as resistant by the automated system despite being sensitive by the reference method), and very major errors (VME, results falsely categorized as sensitive by the automated system despite being resistant by the reference method) were calculated. Results: Of the 236 isolates, colistin resistance was found in 34 (14.4%) isolates, with A. baumannii, K. pneumoniae, P. aeruginosa, and E. coli exhibiting resistance rates of 15.2%, 20.6%, 1.8%, and 23.8%, respectively. A. baumannii showed 89.1% CA between the two methods, with 10.8% VME. K. pneumoniae had an 83.8% CA with a 14.7% VME rate. P. aeruginosa exhibited a 98.1% CA with a 1.8% VME rate, while E. coli showed an 80.9% CA with a 19% VME rate. The BD Phoenix 100 automated s ystems hada 10.5% VME rate compared to BMD. The automated system identified 9 out of 34 isolates as susceptible, contrary to the reference method which reported them as resistant. Conclusions: Categorical agreement rates of the automated system varied according to bacterial isolates. The high VME rate of the BD Phoenix 100 system demonstrated the need to confirm colistin results reported as susceptible to SMD. [ABSTRACT FROM AUTHOR]

Serratia marcescens idrar yolu enfeksiyonları, pnömoni, sepsis, menenjit gibi enfeksiyonlara ve hastane kaynaklı enfeksiyonlara da neden olabilen fırsatçı Gram negatif bir bakteridir. En sık çocuk yenidoğan ve yoğun bakım ünitelerinde görülmektedir. Birçok antibiyotiğe doğal dirençlidir.Bu çalışmada Göztepe Prof. Dr. Süleyman Yalçın Şehir Hastanesi Tıbbi Mikrobiyoloji Laboratuvarı’na 2016-2022 yılları arasında çeşitli kliniklerden gönderilmiş örneklerden izole edilen S. marcescens suşlarının piperasilin-tazobaktam, seftriakson, seftazidim, sefepim, meropenem, ertapenem, gentamisin, amikasin, siprofloksasin, ve trimetoprim-sülfametoksazol antibiyotiklerine direnç durumunun belirlenmesi ve direnç oranının yıllar içindeki değişiminin retrospektif olarak incelenmesi amaçlanmıştır.Çalışmaya hastaneye ayaktan başvuran ve hastanede yatan hastaların örneklerinden izole edilen S. marcescens suşları dahil edilmiştir. Örnekler rutin mikrobiyolojik standartlara göre işlenmiş, mikroorganizmaların tanımlanması ve antibiyotik duyarlılıkları otomatize sistemler kullanılarak yapılmıştır.S. marcescens olarak tanımlanan 416 izolatın 239’u servis ve yoğun bakım ünitelerinde yatan hastalardan (%58), 177’si (%42) poliklinik hastalarından gelen örneklerde saptanmıştır. S. marcescens %42 oranıyla en çok üriner sistem örneklerinde izlenmiş, %20’si trakeal aspirat örneklerinden, %13’ü kan kültüründen, %10’u apse aspiratı ve yara sürüntüsü örneklerinden, %5’i balgam, %4’ü doku, %3’ü kateter, %2’si bronkoalveolar lavaj ve %1’i diğer örneklerden izole edilmiştir.Çalışmamızda izole edilen S. marcescens suşlarının en duyarlı olduğu antibiyotiklerin aminoglikozit grubu antibiyotiklerden amikasin (>%98) ve gentamisin (>%96), karbapenemlerden meropenem (>%98) ve ertapenem (>%97) olduğu saptanmıştır. En dirençli olduğu antibiyotiğin ise %15.4 direnç oranıyla seftriakson olduğu bulunmuştur.Sonuç olarak ciddi enfeksiyonlara neden olabilen ve fırsatçı bir patojen olan S. marcescens’in antibiyotik direnç profilinin belirlenmesi tedavi yönetiminde yol gösterici olması ve yapılacak diğer çalışmalara ışık tutması açısından önemlidir.

Objective: This study aimed to determine the epidemiological characteristics of Helicobacter pylori (H. pylori) infection at the genotype level in patients presenting with dyspeptic complaints, and to show the distribution of virulence factors, importance of intrafamilial transmission, as well as the distribution of resistance to macrolide and quinolone antibiotics. Methods: The study comprised 110 patients with dyspeptic complaints who were admitted to our hospital between January 13, 2015, and December 31, 2016. Through histopathology, culture, and glmMpolymerase chain reaction (PCR) techniques, we detected H. pylori. The vacA, cagA, and cagE genes were determined in patients with positive PCR results were positive. Results: H. pylori strains and clinical results were not found to be significantly correlated in the study. Both genetic variants A2142G and A2143G were discovered to be present in the individuals. Eight patients had clarithromycin resistance (34.7%). All patients with a positive A2142G mutation and 55% of patients with a positive A2143G mutation were found to have clarithromycin resistance. Levofloxacin resistance was present in only one (4.3%) patient who could produce H. pylori in culture. Conclusion: Approximately 1/3 of the children with dyspeptic complaints were positive for H. pylori infection. The most common genotype was observed to be vacAs2. Even individuals with at least one of the genetic mutations A2142G and A2143G have the potential for antibiotic resistance. High resistance was found against clarithromycin in the standard triple therapy regimen used in children for treating H. pylori infection. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Anatolian Environmental & Animal Science is the property of Bulent Verep and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective: Urinary tract infections (UTIs) are the most common infections in childhood after upper respiratory tract infections. The aim of our study was to identify the most common microorganisms causing UTIs in children in our region and to determine the antibiotic resistance profiles of the identified uropathogens. Methods: The urine culture results and antibiotic resistance profiles of 4219 pediatric patients diagnosed with UTI between July 2019 and June 2022 were retrospectively evaluated. Bacteria isolated from urine cultures were identified and antibiotic resistance profiles were determined using the fully automated BD Phoenix-100™ system (Becton Dickinson Diagnostics, USA). The results were interpreted according to the European Committee on Antimicrobial Susceptibility Testing (EUCAST) guidelines. Results: The urine culture results showed a positivity rate of 12.9% (546/4219). It was found that 85.7% (468/546) of the isolated microorganisms were gram-negative bacteria. Of the 533 pediatric patients diagnosed with UTIs, 345 (64.7%) were female and 188 (35.3%) were male (p <0.05). The age group with the highest rate of identified uropathogens was 0-2 years old and accounted for 54.8% (n: 292) (p <0.05). The most frequently isolated uropathogens in urine samples were Escherichia coli (53.8%), Klebsiella pneumoniae (13.7%), Klebsiella oxytoca and Enterococcus faecalis (5.1%). The highest antibiotic resistance rates were observed for ampicillin (56.1%) and amoxicillin-clavulanate (42.9%) in Escherichia coli and for cefixime (44.0%) and amoxicillin-clavulanate (41.3%) in Klebsiella pneumoniae. Surveillance of regional antibiotic resistance profiles is important to guide empiric treatment. Conclusions: According to the results of our study, ampicillin, amoxicillin-clavulanate, trimethoprim-sulfamethoxazole and cefixime should not be favored for the empirical treatment of UTIs due to resistance rates above 20. Considering their oral availability and low resistance rates, we suggest that cefuroxime, fosfomycin and nitrofurantoin should be preferred for the empirical treatment of UTIs in our region. [ABSTRACT FROM AUTHOR]

Background: Acute infectious enteritis is one of the most common causes of death among children in developing countries. In developed countries, 75% to 90% of childhood acute gastroenteritis is caused by viruses, and approximately 20% is caused by bacteria. It is known that parasitic agents are responsible for approximately 5-10% of acute infectious gastroenteritis. Metronidazole, a nitroimidazole, is restricted to Clostridium difficile-associated colitis, anaerobic bacteria, protozoans (such as Entamoeba histolytica, Giardia intestinalis, Trichomonas vaginalis) and microaerophiles (such as Helicobacter pylori). The use of metronidazole in patients with enteritis without a microbiological diagnosis may lead to increased antibiotic resistance in bacteria, unnecessary costs, adverse events and intestinal dysbiosis. Materials and Methods: In our hospital, all stool samples examined from patients under 18 years of age in 2020 and 2021 were tested using FilmArray GI Panel (Biomerioux, France), direct microscopy of stool, rapid antigen tests (Rotavirus, Adenovirus, G. intestinalis, Cryptosporidium spp, C. difficile toxin A/B ( CerTest Biotech, Spain)), E. histolytica microELISA (Adhesin Ag, Entamoeba CELISA Path, Australia) results and stool cultures were scanned retrospectively. Metronidazole usage status of the patients was obtained from the Hospital Information Management System. Results: With the diagnostic methods used in this study, stool samples of 2282 patients were evaluated over a two-year period and 782 factors were detected in 663 (29%) patients. The most frequently detected agent was Rotavirus with a rate of 11.6%. There were 564 (24.7%) patients with a single agent, 77 (3.3%) patients with two agents, and 22 (0.9%) patients with three agents. While 124 (18.7%) of the patients used metronidazole, no factor requiring the use of this drug was detected in 47 (7%) of them. Conclusions: As a result, detecting the microorganisms that cause gastroenteritis with appropriate diagnostic methods will contribute to patients receiving appropriate treatment and preventing unnecessary antibiotic use and antibiotic resistance. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Faculty of Pharmacy of Ankara University / Ankara Üniversitesi Eczacilik Fakültesi Dergisi is the property of Ankara University and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of ANKEM Antibiyotik & Kemoterapi Dergisi is the property of ANKEM Antibiyotik & Kemoterapi Dergisi and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Eurasian Journal of Veterinary Sciences is the property of Eurasian Journal of Veterinary Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Pediatric Infection / Çocuk Enfeksiyon Dergisi is the property of Journal of Pediatric Infection / Cocuk Enfeksiyon Dergisi and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Pediatric Infection / Çocuk Enfeksiyon Dergisi is the property of Journal of Pediatric Infection / Cocuk Enfeksiyon Dergisi and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Uludag University Medical Faculty / Uludağ Üniversitesi Tıp Fakültesi Dergisi is the property of Journal of Uludag University Medical Faculty and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Agriculture & Nature / Kahramanmaraş Sütçü İmam Üniversitesi Tarım & Doğa Dergisi is the property of Kahramanmaras Sutcu Imam Universitesi and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective: The aim of this study was to evaluate the change in methicillin resistance and antibiotic susceptibility of staphylococcal strains isolated from various clinical samples of patients hospitalized in intensive care units over the years. Materials and Methods: A total of 5144 staphylococcal strains isolated from various clinical specimens of inpatients in intensive care units in a university health practice and research hospital medical microbiology laboratory between January 2018 and December 2021 were (96.51%) included in the study. Bacteria identification and antibiotic susceptibility tests were performed using conventional methods and an automated systems. Results: Of the total 5144 staphylococci strains, 4503 were identified as coagulase-negative staphylococci (CNS) (87.54%) and 641 as Staphylococcus aureus (S. aureus) (12.46%). Of these strains, 1451 (28.21%) were isolated from anesthesiology and reanimation intensive care unit, and 4573 (88.90%) from blood samples. The most dramatic increase in both S. aureus and CNS in 2021 occurred in levofloxacin (S. aureus 100%; CNS 99.45%) and ciprofloxacin (S. aureus 100%; CNS 98.54%). While a high rate of resistance to fusidic acid (96.51%) was observed in CNSs, the resistance, which was over 65% in S. aureus strains, decreased significantly (5.40%) in 2021. The lowest antibiotic resistance was in daptomycin, linezolid, and teicoplanin in both bacterial groups. Methicillin resistance, which was 62.31% in 2018 in S. aureus strains, decreased to 33.10% in 2021, while it decreased from 86.68% to 75.06% in CNSs. The rate of resistance to all the examined antibiotics were found to be significantly higher in CNSs. Conclusion: To prevent the development of resistance, antimicrobial resistance profiles should be determined through effective surveillance studies, and each hospital should establish its own antibiotic use policy. Additionally, it is important to ensure the rational use of antibiotics and to take effective infection control measures. [ABSTRACT FROM AUTHOR]

Introduction: Inappropriately prescribed antibiotics accelerate the formation of antibiotic-resistant bacteria. This circumstance creates a problematic situation in terms of public health. This study aims to measure dentists' antibiotic/prophylaxis knowledge and awareness of antibiotic resistance in Turkey based on two case scenarios. Materials Methods: Dentists in Turkey are invited by open invitation posts on various social media applications for our twelve-question online survey. The scoring was shaped based on answers to measure antibiotic/prophylaxis knowledge. The collected data were statistically analyzed by SPSS Statistics 22. Results: 321 participants answered all questions included in the study. The statistical analyses showed a significant difference in the evaluations according to gender, title, graduation time, and workplace. Conclusion: Inappropriate antibiotic prescription by dentists is common in Turkey. Dentists should receive more undergraduate lessons, and taking reminder antibiotic courses at regular intervals after graduation can contribute to keeping their knowledge up to date.

Copyright of Journal of Literature Pharmacy Sciences is the property of Turkiye Klinikleri and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of ANKEM Antibiyotik & Kemoterapi Dergisi is the property of ANKEM Antibiyotik & Kemoterapi Dergisi and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Bosphorus Medical Journal / Boğaziçi Tıp Dergisi is the property of KARE Publishing and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective: Repeat breeder, which is one of the reproductive system disorders seen in cattle, is defined as the inability to conceive with three or more matings with true heat, is seen to be a breeding problem that causes serious economic losses all over the world with a decrease in reproductive efficiency and therefore a decrease in milk production. The aim of this study is to identify the aerobic microflora and antibiotic resistance profiles of dominant bacterial groups in the genital tract of repeat breeder cows that do not exhibit clinical signs of any genital system infections using conventional cultural methods. Methods: The study material comprised 32 repeat breeder cows of Holstein breed aged between four and nine years (with three or more inseminations). Specimens were collected by sterile swab. The collected swab samples were taken into tubes with Fluid Thioglycollate Medium and delivered to the laboratory under cold chain conditions. Specimens for bacteriological examination were incubated at 37 °C for 24 h. After the end of incubation, isolation and identification of colonies growing on EMB agar and blood agar medium were performed. Results: Bacteriological isolation and identification of genital canal samples revealed the isolation of 14 bacterial species mainly from the phylum Protobacteria, along with Firmicutes and Actinobacteria filaments. Among the isolated bacterial species, Escherichia coli (21.4%), Stenotrophomonas maltophilia (14.3%), Staphylococcus haemolyticus (11.9%), Staphylococcus sciuri (7.1%), Corynebacterium psuedodiphteriticum (7.1%), and Corynebacterium bovis (4.8%) were identified. In repeat breeder cows. Some of the isolated bacteria were associateted with subclinical endometritis leading to infertility in repeat breeder cows. Susceptibility of eight antibiotics against dominant bacterial groups and infertility-related bacteria were examined by antibiogram. Conclusion: It was determined that bacterial isolations such as C. bovis and C. psuedodiphteriticum from the Actinobacteria phylum and S. sciuri and S. haemolyticus from the Firmicutes phylum in samples taken from cows identified as repeat breeders are as significant as E. coli from the Protobacteria phylum. Ceftiofur demonstrated 100% effectiveness against the predominant pathogen E. coli in the vaginal microbiome. [ABSTRACT FROM AUTHOR]

This study addresses the need for sustainable methodologies in antimicrobial resistance (AMR) surveillance, particularly in wastewater treatment, to ensure efficient disinfection and control of AMR. The use of photocatalysis (PC) has gained attention as a scalable and suitable approach for research and development. This study evaluates the effect of UV-vis driven sub-lethal photocatalytic oxidation on conjugative gene transfer between two E. coli strains using a pilot plant reactor system. Photocatalysts composed of graphene-oxide-Ti- CuFe2O4 nanocomposites were synthesized through a green approach and used to enhance bacteria inactivation rates, resulting in hindered frequency and absolute abundance of trans-conjugants in the recipient strains. Experiments plan was built with the intent to determine the contribution of photocatalyst type, mode of operation on the conjugation mechanism and also distinguish between the scenarios where individual or simultaneous exposure to PC oxidation of donor and recipient strains occur. Simultaneous photocatalytic treatment of both donor and recipient strains resulted in the removal of ~3 LOG of both bacteria and eligible conditions were obtained for controlling trans-conjugants formation compared to no treatment conditions. The photocatalyst surface, reactive oxygen species, and bacterial cells' interaction played a determining role in controlling ARG transfer. The impact of photocatalytic oxidation mechanisms on the vitality of recipient cells was evident during the continuous mode of operation, where conjugative transfer of ARGs was mitigated, and the number of trans-conjugants decreased to below 102 CFU mL-1. This study demonstrates the potential of PC for efficient disinfection and control of AMR in wastewater treatment. [ABSTRACT FROM AUTHOR]

Aim: In this study, it was aimed to establish an infection model with Klebsiella pneumoniae on A549 Lung cancer cell line and to evaluate the effect of additional vitamins on the antibacterial effect of ampicillin sulbactam in the treatment. Cell culture and pathology results were determined in parallel with microbial analysis. Methods: Minimal inhibitory concentration (MIC), fractional inhibitory concentration (FIC), biofilm optical density of ampicillin sulbactam, and vitamins E, K1, and P on Klebsiella pneumoniae ATCC 700603 strain were determined. Cytotoxic activity on A549 cancer cell line in parallel with microbial analysis and pathology results were determined. Results: Ampicillin Sulbactam MIC dose range used in the treatment of Klebsiella pneumoniae infection is 16 mg/L. Looking at the FIC dose ranges, Vitamin K1+Ampicillin sulbactam, Vitamin P+Ampicillin sulbactam 2.5mg/ml+0.5µg/ml, Vitamin E+Ampicillin sulbactam 5mg/ml+0.5µg/ml, K1+P+E+Ampicillin sulbactam 2,5mg/ml+2.5mg/ml+5mg/ml+0.5µg/ml it was determined that it had a synergistic effect in combination with vitamins K1 and P antibiotics. In addition, in the presence of biofilm below 570 nm, the OD values of K1, P, E, and K1+P+E+ antibiotic combinations were found as 1.006, 0.969, 1.096, and 1.015, respectively. As a result of cell culture studies and evaluation of pathology results, it was determined that increasing the dose of Ampicillin sulbactam to 8 µg/ml increased the antibacterial effect, and the vitamin combination was more effective. Conclusions: The antibiotic and vitamin combination was found more effective in Klebsiella pneumoniae infection in our study. This situation contributes to the search for alternative antimicrobials. [ABSTRACT FROM AUTHOR]

Background: The causative agents and antibiotic resistances causing neonatal sepsis can vary according to the units. In this study, we aimed to analyze infants diagnosed with culture-confirmed late-onset neonatal sepsis in terms of frequency, etiologic factors, causative microorganisms and antibiotic resistance. Materials and Methods: The data of the patients monitored in the Neonatal Intensive Care Unit of Şanlıurfa Training and Research Hospital between 2018 and 2023 were obtained from infection control committee records, patient files, and the hospital data system. Among 1641 hospitalized cases during the specified period, 218 cases diagnosed with culture-confirmed late-onset neonatal sepsis were included in the study. Results: 218 cases that were detected late-onset neonatal sepsis out of 1641 blood cultures examined for sepsis suspicion were included during the study period. 76.1 % of the cases were receiving invasive mechanical ventilator support. 23.9 % had picc lines, 55.5% had umbilical catheters, and 68.8 % had total parenteral nutrition. Among the isolates obtained from blood cultures taken after the third day of the study, 67 % were gram-negative agents. Among the gram negative agents, 43.1% were Klebsiella pneumoniae. Mortality rates were significantly higher in female cases and those receiving central venous catheters and invasive mechanical ventilator support. When the causative agents of sepsis were analyzed, mortality rates were significantly higher in cases with gram-negative agents (p < 0.05). For Klebsiella pneumoniae and Escherichia coli, the resistance rates were found to be, respectively: 96.6% and 40% for Ampicillin, 83.1% and 55% for Gentamicin, 72.9% and 75% for Ceftazidime, 25.4% and 10% for Amikacin, 16.9% and 10% for Imipenem/Meropenem, 6.8% and 0% for Colistin, and 5.1% and 15.8% for Levofloxacin. Conclusions: The microorganisms detected in neonatal intensive care units and antibiotic resistance of these microorganisms may be variable. It is known that sepsis-related mortality rates can be reduced with appropriate empirical treatment, in early recognized late-onset neonatal sepsis cases. Therefore, each unit is recommended to formulate its treatment strategy as the sepsis agents and antibiotic resistances may differ among neonatal units. [ABSTRACT FROM AUTHOR]

Background: Intensive care units are the most frequently affected areas by infections caused by Acinetobacter baumannii. Due to its rapid development of antimicrobial resistance, causes serious therapeutic problems. The aim of this study is to determine antibiotic susceptibilities of A. baumannii strains isolated from intensive care units over a four-year period. Materials and Methods: A. baumannii strains isolated from various samples sent from intensive care units to the medical microbiology laboratory between January 2018 and December 2021 were included in the study. Bacterial identification and antibiotic susceptibility tests were performed using standard microbiological methods and automated systems. Antibiotic resistance data from 2018 to 2021 were compared using the Chi-square and Fisher's Exact test, and p<0.05 was considered statistically significant. Results: A total of 1900 A. baumannii strains were included in the study. These strains were most commonly isolated from tracheal aspirate culture samples (1011; 53%). Among the intensive care units, it was determined that it was most frequently isolated from the Anesthesiology and Reanimation intensive care unit (697; 37%). When comparing the years 2018 and 2021, an increase in antibiotic resistance was observed in A. baumannii. The most significant resistance increase was found in amikacin, trimethoprim/sulfamethoxazole, and levofloxacin (p<0.001). Conclusions: Our study revealed high antibiotic resistance among A. baumannii strains. Regular monitoring and reporting of antibiotic susceptibility results will enable physicians to establish antibiotic usage policies and take infection control measures. [ABSTRACT FROM AUTHOR]

Background: Intensive care units (ICU) are multidisciplinary departments where patients with life-threatening diseases, major surgical interventions, respiratory failure, coma condition, hemodynamic insufficiency, and ≥1 organ failure are admitted for relevant diagnoses and treatment. The present study sought to investigate pathogens causing infections in patients admitted to our respiratory ICU and their antibiotic resistance patterns. Materials and Methods: The antibiogram results and clinical data of all patient samples submitted between January 1, 2008, and December 31, 2010, were retrospectively reviewed. Ethics committee approval was obtained from Dicle University Faculty of Medicine. Results: In total, 248 patients with 561 culture results were included in the study. Microbial growth was detected in the following samples: blood, 336 (59.9%); deep tracheal aspirate, 104 (18.6%); urine, 89 (15.9%); wound drain, 12 (2.1%); central venous catheter liquid, 7 (1.3%); phlegm, 10 (1.8%); Foley tip liquid, 1 (0.2%); and pleural effusion, and 1 (0.2%). Rapid growth was most frequently noted in the cultures of coagulase-negative staphylococci (25.3%), Acinetobacter spp. (23.1%), and Escherichia coli (12.6%). Conclusions: the present study revealed microorganisms' resistance profiles similar to those of other relevant studies. The study provides important insights into the selection of empiric antibiotic therapy for patients admitted in intensive care unit. [ABSTRACT FROM AUTHOR]

The variety of microorganisms contained in raw milk and their resistance profile potentially pose a danger to human health and the food industry. This study was aimed to investigate pathogen content of milk samples obtained from healthy animals and the prevalence of Streptococcus, a common contaminant of milk, and antibiotic resistance profiles. In total, 358 isolates, including Staphylococcus spp., Streptococcus spp., Lactococcus spp, Escherichia coli, Klebsiella oxytoca, Enterococcus faecalis and Macrococcus caseolyticus, were obtained from 249 milk samples. The prevalence of streptococci was found 24.58%. Streptococcal isolates were found most resistant to the aminoglycoside class, followed by quinolone, tetracycline, macrolide, beta lactam, nitrofuran and phenicol resistance respectively. This situation has shown that the consumption of raw milk can be a potential risk for foodborne infection. Hence, it was thought that consumers should avoid unpasteurized milk and dairy products, monitor raw milk more in terms of antibiotic resistance, and take the necessary precautions. [ABSTRACT FROM AUTHOR]

Objective: Streptococcus agalactiae is a serious invasive infectious agent that can be colonized in gastrointestinal and genitourinary system in healthy individuals, and may cause high mortality in newborns, pregnant women and those with underlying disease. In this study, it was aimed to investigate the growth rates of S. agalactiae isolated from urine cultures and to determine their antibiotic susceptibility. Material and Method: S. agalactiae isolates recovered from urine cultures between June 2016 and April 2019 were investigated retrospectively. Conventional methods were used for the identification. Antibiotic susceptibility tests were performed and evaluated according to Clinical and Laboratory Standards Institute recommendations. Results: S. agalactiae was detected as a single agent in 163 (0,30%) of 54.009 urine cultures. In total, S. agalactiae was isolated in 29 (0,14%) of 19.794 male patients 134 (0,39%) of 34.215 female patients. Among female patients, 18 (0,62%) of 2.883 were pregnant. It was determined that 95% of the patients were outpatients and 35% were from the Obstetrics and Gynecology Unit. The isolates were 100% sensitive to penicillin, ampicillin, cefotaxime and vancomycin; while 10,6% of the strains were resistant to erythromycin and 24,3% of the strains were resistant to to ofloxacin. Conclusion: S. agalactiae is less frequently isolated from urinary tract infections. Prophylaxis and treatment are recommended especially for risk group. In our study, the increased resistance rates of erythromycin and ofloxacin is notable. Routinely follow-up of S. agalactiae isolation from clinical samples, especially from urine cultures, evaluation of the clinical symptoms of patients (particularly in pregnant and/or people with underlying diseases) and monitor antibiotic resistance for early and appropriate treatment are important. [ABSTRACT FROM AUTHOR]

Copyright of Hamidiye Medical Journal is the property of Galenos Yayinevi Tic. LTD. STI and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Pediatric Infection / Çocuk Enfeksiyon Dergisi is the property of Journal of Pediatric Infection / Cocuk Enfeksiyon Dergisi and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Kocatepe Veterinary Journal / Kocatepe Veteriner Dergisi is the property of Afyon Kocatepe University, Faculty of Veterinary Medicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Ahi Evran Medical Journal is the property of Ahi Evran University and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Aim: The aim of this study was to compare the resistance to asymptomatic bacteriuria (ABU) between term and preterm pregnancies. Material and Methods: Pregnant women aged 17-41 years who were followed up in the obstetrics department of our hospital and whose urine cultures were sent to the laboratory for analysis and reported as ABU were included in the study. Patients were divided into two groups according to gestational weeks as preterm (

Amaç: Bu çalışmada, 2020 yılı içerisinde hastanemizin yoğun bakım üniteleri ve yataklı servislerinden mikrobiyoloji laboratuvarına gönderilen çeşitli kültür örneklerinden izole edilen gram negatif bakteriler ve antibiyotik dirençlerinin retrospektif olarak değerlendirilmesi amaçlanmıştır. Metod: Ocak 2020-Aralık 2020 tarihleri arasında yoğun bakım üniteleri ve yataklı servislerden mikrobiyoloji laboratuvarına gönderilen çeşitli hasta örneklerinden sık izole edilen Gram negatif bakteriler çalışmaya dahil edilmiştir. Bakteri tanımlanması ve antibiyotik duyarlılık testleri konvansiyonel yöntemler ve otomatize sistemler kullanılarak yapılmıştır Bulgular: Bir yıllık süreçte yoğun bakım ünitelerindeki hastalardan sırasıyla %49.7’u endotrakeal aspirat kültürlerinden izole edilen 761 Acinetobacter baumanii, %51.7’si idrar kültüründen izole edilen 478 E. coli, %31.2’si endotrakeal aspirat kültürlerinden izole edilen 417 Klebsiella pneumoniae, %51.5’i endotrakeal aspirat kültürlerinden izole edilen Pseudomonas aeruginosa üremesi olmuştur. Yataklı servislerde ise %47.2’si idrar kültüründen izole edilen 316 Escherichia coli, %43.4’ü idrar kültürlerinden elde edilen 136 Klebsiella pneumoniae, %57.7’si yara kültürlerinden izole edilen 78 Pseudomonas aeruginosa, %60.3’ü yara kültürlerinden izole edilen 68 Acinetobacter baumanii üremesi olmuştur. Yoğun bakımlarda üreyen Gram negatif bakteriler servislerde üreyenlerden anlamlı derecede yüksek bulunmuştur (P lt;0.005). En fazla Acinetobacter baumanii izolatlarında hem yoğun bakımlarda hem de yataklı servislerde çoğu antibiyotiğe %100’ yakın direnç görülmüştür. Sonuç: Yoğun bakım ünitelerinde Acinetobacter baumanii en çok izole edilen ve hem yoğun bakımlarda hem de yataklı servislerde yatan hastalarda yüksek oranda antibiyotiğe direnç gösteren bakteri olmuştur. YBÜ başta olmak üzere hastanelerde Gram negatif bakterilerin dağılım özellikleri ve ilaç direnç oranları hakkındaki bilgileri kavramak, antibiyotiklerin doğru ve etkin kullanımının yanı sıra ilaç direnç oranlarının da azaltılabilmesi için oldukça önemlidir.

Copyright of Selcuk Dental Journal is the property of Selcuk Dental Journal and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective: This study, it was aimed to investigate the antibiotic susceptibility of Escherichia coli (E. coli) isolates isolated from different samples sent to the microbiology laboratory of our hospital in five years retrospectively. Material and Method: 3422 E. coli strains isolated from different samples sent to Malatya Turgut Özal University Faculty of Medicine Malatya Training and Research Hospital Microbiology Laboratory between January 2015 and December 2019 was evaluated retrospectively within the scope of this study. Identification and antimicrobial susceptibility tests (ADT) of all isolates were performed with the VITEK 2° automated system. Antimicrobial susceptibility tests and interpretation of results were made in accordance with the recommendations of The European Committee on (EUCAST). Antimicrobial Susceptibility Testing Results: According to the ADT results of 3422 E. coli strains included in the study, the antibiotics with the highest resistance rates were respectively; ampicillin (67.1%), cefuroxime axetil (44.9%) amoxicillin/clavulanic acid (42.5%), trimethoprim/sulfamethoxazole (41.7%), ceftazidime (39.6%) and ciprofloxacin (39.7%) were determined. The antibiotics with the lowest resistance rates, respectively; carbapenems (imipenem 0.6%, meropenem 0.6%, ertapenem 2.7%), fosfomycin (2.5%), nitrofurantoin (2.5%) and amikacin (6.3%). Conclusion: The resistance rates detected in this study were found to be similar to those in our country. We believe that these data will guide the treatment of E. coli infections and contribute to the success of treatment. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Faculty of Pharmacy of Ankara University / Ankara Üniversitesi Eczacilik Fakültesi Dergisi is the property of Ankara University and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Purpose: The aim of this study was to evaluate the clinical and demographic characteristics and resistance patterns to antistaphylococcal antibiotics of Staphylococcus aureus (S. aureus) isolates, which is a leading cause of invasive infections in children. Materials and Methods: Patients who were under 18 years of age and who had S. aureus growth in abscess, wound, blood, cerebrospinal fluid, joint fluid and any other sterile body fluid cultures, who were followed up at Children’s Health and Diseases Clinic in between July 2018 and July 2020 were included in the study. Results: A total of 135 patients and cultures were included in the study. Community-acquired S. aureus (CA-SA) infection was present in 105 (77.7%) patients and hospital-acquired S. aureus (HA-SA) infections in 30 (22.3%). S. aureus was most commonly detected in skin and soft tissue infections. Skin and soft tissue infections were more common in patients with community-acquired disease, whereas bacteremia was more common in patients with nosocomial infection. Methicillin-resistant S. aureus (MRSA) isolates was encountered in 53.3% of all patients; Clindamycin resistance was found in 20% of all staphylococcal isolates and the ratio of mupirocin resistance was 14.4%.55.8% of all CA-SA and 46.7% of all HA-SA isolates were MRSA. Penicillin, mupirocin, erythromycin, and tetracycline resistance were significantly higher in MRSA isolates as compares to non-MRSA isolates. Mupirocin resistance was significantly higher in CA-SA isolates. The median length of hospital stay was 12 days. Length of hospital stay, duration of intravenous antibiotics use, mortality, and clindamycin resistance were significantly higher in patients with HA-MRSA infections. While overall mortality was 2.2% in patients with S. aureus infections, when evaluated separately, it was 10% in patients with HA-SA infections. Conclusion: Our CA-MRSA rates are quite high as compared to other reports, and clindamycin and mupirocin resistance seems to be an important problem in our region. Taking appropriate cultures at the right time is important in determining resistance patterns and guiding empirical treatment regimens.

Aim: The aim of this study is to examine the effect of the changing microbiota structure during the pandemic period on the bacterial aerobic culture profile of the wound taken from patients operated for acute appendicitis, together with clinical variables. Materials and Methods: Our study included 125 patients who underwent an appendectomy in the General Surgery Clinic between 01.03.2019-01.02.2021 and whose wound culture was taken during an appendectomy. The patients were divided into two groups; group1 (pre-pandemic) and group2 (during a pandemic). Both groups were compared in terms of age, gender, clinical, laboratory and wound culture data. Results: There was no significant difference between the groups in terms of age, clinical symptoms, surgical method, laboratory and radiological data (p>0.05). In the pre-pandemic group, there was growth of E. coli in 35 (53%) patients, K. pneumoniae in 3 (4.5%) patients, and P. aeruginosae in 2 (3) patients from Gram-negative bacteria while Strep. anginosus and Strep. constellatus growth were most common from Gram-positive bacteria. In the pandemic group, there was the growth of E. coli in 29 (49.2%) patients, P. aeruginosae in 5 (8.5%) patients, K. pneumoniae in 2 (3.4%) patients from Gram-negative bacteria, Citrobacterfreundii and Streptococcusanginosus growth were the most common from Gram-positive bacteria. Escherichiacoli is the most common bacteria in both groups. Conclusion: Although E. coli was found to be the most frequently identified microorganism in patients with acute appendicitis, an increase in the density and resistance of Pseudomonas group bacteria were detected, possibly due to the effect of the COVID-19 pandemic.

Copyright of Online Turkish Journal of Health Sciences (OTJHS) / Online Türk Sağlık Bilimleri Dergisi is the property of Oguz KARABAY and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Faculty of Pharmacy of Ankara University / Ankara Üniversitesi Eczacilik Fakültesi Dergisi is the property of Ankara University and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of ANKEM Antibiyotik & Kemoterapi Dergisi is the property of ANKEM Antibiyotik & Kemoterapi Dergisi and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Health Sciences of Kocaeli University / Kocaeli Üniversitesi Sağlık Bilimleri Dergisi is the property of Institute of Health Sciences of Kocaeli University and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Health Sciences of Kocaeli University / Kocaeli Üniversitesi Sağlık Bilimleri Dergisi is the property of Institute of Health Sciences of Kocaeli University and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Mersin Üniversitesi sağlık Bilimleri Dergisi is the property of Mersin University and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective: Incidence of ventilator-associated pneumonia, caused by gram-negative multidrug resistant bacteria, is on the increase and early mortality rate is high. We aimed to investigate the effects of aging, comorbidities, high Charlson comobidity index score, high Acute Physiology and Chronic Health Evaluation II score, leukocytosis, high C-reactive protein level, inappropriate empirical antibiotic therapy and antibiotic resistance on mortality in ventilator-associated pneumonia caused by gram-negative multidrug-resistant bacteria. Methods: The study was planned as a retrospective cohort study. Patients aged 18 years and older who were hospitalized between January 01, 2015, and January 01, 2020, diagnosed with ventilator-associated pneumonia, and had Gram-negative multidrug resistant pathogen was detected in blood and/or bronchoalveolar lavage fluid specimen or quantitative endotracheal aspirate cultures were included in the study. Results: A total of 370 patients were included in the study. Median age of the patients was 74 (19-95) years. Most frequent bacteria was Acinetobacter baumannii (52.4%). Resistance to ceftriaxone, meropenem, and colistin was 99%, 68%, and 4%, respectively. 7-day and 21-day mortality rates were 38.3% (n=142) and 85.1% (n=315). In multivariate analysis, 7-day mortality was associated with a Charlson comorbidity index score of =4, and risk factors for 7-day mortality were septic shock, amikacin resistance, and white blood cell count =15000/mm3. Advanced age was found to be a risk factor for 21-day mortality, and a high Acute Physiology and Chronic Health Evaluation II score and a high Charlson comorbidity index score were associated with 21-day mortality. It was found that the risk of 7-day mortality in patients with tracheostomy was lower than in patients without tracheostomy. Conclusion:Consideration of clinical scoring systems, closer monitoring of elderly patients, following-up with tracheostomy, may provide a decrease in mortality of ventilator-associated pneumonia, caused by multidrug resistant pathogens. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Agriculture & Nature / Kahramanmaraş Sütçü İmam Üniversitesi Tarım & Doğa Dergisi is the property of Kahramanmaras Sutcu Imam Universitesi and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Avian pathogenic Escherichia coli (APEC) causes systemic or localized infections with different clinical courses such as septicemia, air sac disease, polyserositis and coligranuloma. Colibacillosis causes significant economic losses in the poultry industry due to the need for the control of the disease by causing carcass contamination, decreased feed conversion rate, mortality in poultry. In this study, 99 E. coli strains were isolated from different production units in the same integration. Antimicrobial susceptibility tests of all strains were performed and thirty strains with multidrug resistance (MDR) in the different production units were serotyped. The highest resistance was observed against oxytetracycline, erythromycin amoxicillin and doxycycline. Multidrug resistance was observed at a rate of 81.81% (81/99). Among the 30 strains that could be serotyped, O78 and O125 were determined as the most prevalent serogroups with 43.3% (13/30) and 16.6% (5/30) rates, respectively, while O1, O8, O18, O142, O143, O157, O158, O164 and O169 were found as rare serogroups. O78 was determined as a high antibiotic resistant strain in isolated E. coli strains and a dominant serotype in the selected strains and this study demonstrated that a correlation can be between breeders and their progeny. [ABSTRACT FROM AUTHOR]

Objective: Nosocomial infections are among the important causes of mortality and morbidity in hospitalized patients. Infections seen in intensive care units (ICUs) constitute 25% of nosocomial infections, and respiratory system infections are the most common among them. The pathogens isolated from these infections and antibiotic resistance of them may differ amonghospitals, as well as within the same unit over time. Endotracheal aspirate (ETA) cultures is the most widely used microbiological diagnosis method in respiratory tract sampling all over the world. In this study, it was aimed to retrospectively investigate bacterial agents and antibiotic resistance rates isolated from ETA samples of patients in the ICU of Süleyman Demirel University. Material and Method: Bacterial agentsisolated from ETA samples of ICU patients between January 2018 and January 2021 and antibiotic susceptibility test (ADT) results of these agents were obtained retrospectively from the hospital database. Sensitivity results were evaluated in accordance with the recommendations of the European Committee on Antimicrobial Susceptibility Testing (EUCAST) and, when needed, the Clinical & Laboratory Standards Institute (CLSI). Repeated growths from the cultures of the same patient were not evaluated. Results: During the evaluated period, a total of 458 (44.4%) bacteria were isolated from 1031 ETA samples, of which 413(90.2%) were gram negative and 45(9.8%) were gram positive. Acinetobacter baumannii (43.9%) was the most common gram-negative bacterium and Staphylococcus aureus (5.9%) was the most common gram-positive bacterium. It was determined that the isolated A. baumannii strains showed high resistance to many antibiotics in all years, and the highest resistance was detected against the carbapenem group with a rate of 96.6%. Extended-spectrum betalactamase (ESBL) positivity was detected in 48.1% of Escherichia coli strains and 70.8% of Klebsiella pneumoniae strains. In our study, Methicillin-resistant Staphylococcus aureus (MRSA) was detected at a rate of 20.7%, and methicillin-resistant Coagulasenegative Staphylococci (CNS) was detected at a rate of 76.9%. Conclusion: The specific feature of infections occuring in patients hospitalized in the ICU is that antibiotic resistant pathogens are often the cause. Identification of the causative agent of the infection, determination of the antibiotic resistance profile will guide the antibiotic selection and will contribute to the prevention of inappropriate antibiotic use in the ICU and to reduce the resistance rates. [ABSTRACT FROM AUTHOR]

Objective: Determining colistin susceptibility according to the reference method is a significant problem for clinical microbiology laboratories. There is a need for a fast, easy-to-apply, and accurate method for laboratories with limited opportunities. In our study, we aimed to evaluate the applicability of the colistin broth disk elution (CBDE) test. Methods: A total of 193 Gram-negative bacteria, which were isolated from various clinical specimens in our laboratory, were included in the study. Colistin susceptibility was determined by broth microdilution as the reference method. The CBDE test was performed as previously described. For each bacterial isolate, 0, ½, 1, 2, and 4 colistin discs (10 µg; BD) were added to 5 tubes containing cation-adjusted Mueller-Hinton broth (MHB). The final colistin concentrations in the tubes were 0 (growth control), 0.5, 1, 2, and 4 µg/ml, respectively. The minimum inhibitory concentration values were determined by visual evaluation 16-20 hours after the bacterial inoculation. Results: In the Enterobacterales group, the categorical agreement was 94%, the major error was 5%, and the very major error was 0%. In the non-fermentative bacteria group, the categorical agreement was found to be 94%, the major error 1%, and the very major error 33%, respectively. Conclusion: The CBDE test is a good alternative to broth microdilution in routine microbiology laboratories for the Enterobacterales group since it provides easy-to-apply, cost-effective testing for colistin susceptibility and has a high categorical agreement compared to the reference microdilution method. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Ankara University Faculty of Medicine / Ankara Üniversitesi Tip Fakültesi Mecmuasi is the property of Galenos Yayinevi Tic. LTD. STI and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)