61 results on '"Anthony J. Keller"'
Search Results
2. Re-examining blood donor deferral criteria relating to injecting drug use
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Alexander J. Thompson, Margaret Hellard, David Wilson, Lisa Maher, Annie Madden, Anthony J. Keller, Clive R. Seed, Joanne Pink, Brendan Quinn, Michael Farrell, Jennifer Williams, and Sharon Caris
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Drug ,medicine.medical_specialty ,Internationality ,Sexual Behavior ,media_common.quotation_subject ,Best practice ,Medicine (miscellaneous) ,Blood Donors ,Guidelines as Topic ,030204 cardiovascular system & hematology ,Expert committee ,Scientific evidence ,Drug Users ,03 medical and health sciences ,Risk-Taking ,0302 clinical medicine ,Epidemiology ,Prevalence ,Humans ,Medicine ,030212 general & internal medicine ,Substance Abuse, Intravenous ,Deferral ,media_common ,business.industry ,Health Policy ,Australia ,virus diseases ,Surgery ,Blood donor ,Family medicine ,Blood safety ,Guideline Adherence ,business - Abstract
Potential Australian blood donors are deferred indefinitely if they report a history of injecting drug use (IDU), or for 12 months if they report having engaged in sexual activity with someone who might have ever injected. Given incremental improvements in blood safety, this study sought to examine whether Australia's IDU-related eligibility criteria reflected current scientific evidence, were consistent with international best practice and, if current IDU-related policies were to be changed, how this should happen.An expert committee was formed to review relevant literature with a focus on issues including: the epidemiology of IDU in Australia and key transfusion-transmissible infections (TTIs) among Australian people who inject drugs (PWID); and, 'non-compliance' among PWID regarding IDU-related blood donation guidelines. International policies relating to blood donation and IDU were also reviewed. Modelling with available data estimated the risk of TTIs remaining undetected if the Blood Service's IDU-related guidelines were changed.Very few (1%) Australians engage in IDU, and IDU risk practices are reported by only a minority of PWID. However, the prevalence of HCV remains high among PWID, and IDU remains a key transmission route for various TTIs. Insufficient data were available to inform appropriate estimates of cessation and relapse among Australian PWID. Modelling findings indicated that the risk of not detecting HIV becomes greater than the reference group at a threshold of non-admission of being an active PWID of around 1.8% (0.5-5.1%). Excluding Japan, all Organisation for the Economic Co-operation and Development member countries permanently exclude individuals with a history of IDU from donating.Numerous research gaps meant that the study's expert Review Committee was unable to recommend altering Australia's current IDU-related blood donation guidelines. However, having identified critical knowledge gaps and future areas of research, the review made important steps toward changing the criteria.
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- 2017
3. Reconsideration of blood donation testing strategy for human T-cell lymphotropic virus in Australia
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Anthony J. Keller, Clive R. Seed, C. E. Styles, Silvana Gaudieri, and Veronica C. Hoad
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Test strategy ,medicine.medical_specialty ,Blood Safety ,Cost-Benefit Analysis ,T cell ,Blood Donors ,Disease ,030204 cardiovascular system & hematology ,Antibodies, Viral ,Risk Assessment ,Virus ,03 medical and health sciences ,Blood donations ,0302 clinical medicine ,Prevalence ,medicine ,Humans ,Blood Transfusion ,030212 general & internal medicine ,Intensive care medicine ,Immunoassay ,Human T-lymphotropic virus 1 ,Hematologic Tests ,Minimal risk ,business.industry ,Australia ,Hematology ,General Medicine ,HTLV-I Infections ,Virology ,medicine.anatomical_structure ,Blood donor ,Blood safety ,business - Abstract
Background and Objectives Universal testing of blood donations for human T-cell lymphotropic virus (HTLV) in Australia may no longer be appropriate given the low prevalence of HTLV infection and the mitigating effect of universal leucodepletion for cellular components. This study aimed to determine the most appropriate HTLV testing strategy using the Risk-Based Decision-Making Framework for Blood Safety. Materials and Methods The risk of HTLV transfusion–transmission using three testing strategies (universal, new-donor and no testing) and cost-effectiveness of the first two strategies were assessed using adaptations of published mathematical models. Results The overall prevalence for 2004–2014 was three HTLV-positives per million donations. It was estimated that annually, universal testing incurred a cost of approximately AUD $3 million and prevented 83 HTLV-positive cellular components from being issued, and new-donor testing cost approximately $225 000 and prevented 81 components. The number of cases of transfusion-transmitted HTLV and HTLV-associated disease prevented per year by universal and new-donor testing was essentially equivalent. According to preset risk thresholds, the risk of transfusion–transmission was negligible for universal and new-donor testing, and minimal without testing. Conclusion Transfusion–transmission of HTLV is a minimal risk in Australia even without testing. However, any revision of testing strategy must consider not only risk and cost-effectiveness, but also stakeholder, ethical and regulatory perspectives. Considering all relevant criteria, new-donor testing is judged the optimal strategy because it is able to achieve almost the same outcomes as universal testing, at a fraction of the cost.
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- 2017
4. Postdonation iron replacement for maintaining iron stores in female whole blood donors in routine donor practice: results of two feasibility studies in Australia
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Denese C. Marks, Hannah E. Salvin, Anthony J. Keller, Sant-Rayn Pasricha, Joanna Speedy, Joanne Pink, and Tania Brama
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medicine.medical_specialty ,Iron replacement ,Immunology ,Population ,Context (language use) ,030204 cardiovascular system & hematology ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Immunology and Allergy ,030212 general & internal medicine ,Adverse effect ,education ,Whole blood ,education.field_of_study ,biology ,business.industry ,Hematology ,Iron deficiency ,medicine.disease ,Surgery ,Ferritin ,Donation ,Emergency medicine ,biology.protein ,business - Abstract
BACKGROUND Iron deficiency represents a risk to donor health and the blood supply. Efficacy trials indicate that postdonation iron replacement improves iron stores but they do not account for complexities of implementation in the routine collection context. We therefore conducted two prospective feasibility studies in Australian donor centers. STUDY DESIGN AND METHODS In both studies we recruited female donors between 18 and 45 years who had made at least one donation in the previous 12 months. In READ (replacement advice), female donors were given a recommendation to self-procure postdonation iron. In DIRECT (donor iron replacement), donors were provided with a course of iron supplements. Donors could return to donate at their discretion and were surveyed after the recruitment visit and again toward the end of the 13-month follow-up. Donor uptake, adverse effects, effectiveness in maintaining iron stores, and workflow impact were assessed. RESULTS We recruited 1404 (70.9% of invited) donors to READ and 768 (53.2% of invited) to DIRECT. READ and DIRECT extended predonation interviews by 1 and 5 minutes, respectively. Among participants, 44 and 88% took iron in READ and DIRECT, respectively. Adverse effects were common but usually mild. READ failed to maintain iron stores in the population, but was effective in donors who consumed more than 75% of the recommended dose. DIRECT was effective in preventing declines in ferritin concentration. CONCLUSION Trade-offs between cost, complexity, uptake, and effectiveness must be considered in the implementation of postdonation iron supplementation.
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- 2017
5. International Forum regarding practices related to donor haemoglobin and iron
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Manish J. Gandhi, André Lebrun, Jed B. Gorlin, Hany Kamel, Harvey G. Klein, J. Faed, J. Chay, Anne F. Eder, Giovani Vandewalle, Johanna Castrén, Whitney R. Steele, Vered Yahalom, D. Teo, Andreas Holbro, Gilles Delage, Rachid Djoudi, Joanne Pink, Veronica Gendelman, Michael J. Germain, J. N. S. Leung, Laura Infanti, Anthony J. Keller, Anne-Marie Fillet, Miquel Lozano, C. C. Y. Chu, Andreas Buser, Gail Miflin, Karin Magnussen, Eilat Shinar, Ralph R. Vassallo, Geneviève Woimant, E. M. O'Neill, Cheuk-Kwong Lee, Kamille A. West, Veerle Compernolle, Hwee Huang Tan, K. van den Hurk, Mindy Goldman, Joanna Speedy, Pierre Robillard, J. M. Cardenas, and Public and occupational health
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education.field_of_study ,medicine.medical_specialty ,Anemia ,business.industry ,Population ,MEDLINE ,Hematology ,General Medicine ,030204 cardiovascular system & hematology ,medicine.disease ,Surgery ,03 medical and health sciences ,0302 clinical medicine ,Donation ,Family medicine ,medicine ,education ,business ,030215 immunology - Published
- 2016
6. Hepatitis E virus RNA in Australian blood donations
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David Warrilow, Jerry A. Holmberg, Clive R. Seed, Robert L. Flower, Ashish C. Shrestha, Anthony J. Keller, Veronica C. Hoad, Hiu Tat Chan, Helen M. Faddy, Robert Harley, and Judith A. Northill
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business.industry ,Transmission (medicine) ,viruses ,Immunology ,virus diseases ,Hematology ,Seroepidemiologic Studies ,030204 cardiovascular system & hematology ,Hepatitis E ,medicine.disease ,medicine.disease_cause ,Virology ,digestive system diseases ,Confidence interval ,03 medical and health sciences ,0302 clinical medicine ,Hepatitis E virus ,Genotype ,Immunology and Allergy ,Medicine ,Seroprevalence ,030212 general & internal medicine ,business ,Viral load - Abstract
BACKGROUND: Hepatitis E virus (HEV) poses a risk to transfusion safety. In Australia, locally acquired HEV is rare and cases are mainly reported in travelers returning from countries endemic for HEV. The risk posed by HEV to transfusion safety in Australia is unknown; therefore, we aimed to measure the rate of current HEV infection in Australian blood donations. STUDY DESIGN AND METHODS: A total of 14,799 blood donations were tested for HEV RNA by transcription-mediated amplification, with confirmatory testing by reverse transcription–polymerase chain reaction. Viral load quantification and phylogenetic analysis was performed on HEV RNA–positive samples. RESULTS: One (0.0068%; 95% confidence interval [CI], 0.0002%-0.0376%) sample was confirmed positive for HEV RNA, resulting in a risk of collecting a HEV-viremic donation of 1 in 14,799 (95% CI, 1 in 584,530 to 1 in 2,657). The viral load in this sample was approximately 15,000 IU/mL, and it was determined to be Genotype 3. DISCUSSION: Our finding of 1 in 14,799 Australian donations positive for HEV RNA is lower than that from many other developed countries; this is consistent with the relatively low seroprevalence in Australia. As this HEV RNA–positive sample was Genotype 3, it seems likely that this infection was acquired through zoonotic transmission, either within Australia or overseas in a developed nation. HEV has the potential to pose a risk to transfusion safety in Australia; however, additional, larger studies are required to quantify the magnitude of this risk.
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- 2016
7. The infectious disease blood safety risk of Australian hemochromatosis donations
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Hiu Tat Chan, Praveen Pathak, Barbara Bell, Peter Bentley, Anthony J. Keller, and Veronica C. Hoad
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medicine.medical_specialty ,business.industry ,Public health ,Immunology ,Retrospective cohort study ,Hematology ,030204 cardiovascular system & hematology ,medicine.disease ,03 medical and health sciences ,0302 clinical medicine ,Infectious disease (medical specialty) ,Internal medicine ,Hereditary hemochromatosis ,Epidemiology ,Immunology and Allergy ,Medicine ,030212 general & internal medicine ,business ,Hemochromatosis ,Whole blood ,Cohort study - Abstract
BACKGROUND It has been suggested that blood donors with hereditary hemochromatosis may pose an increased infectious disease risk and adversely affect recipient outcomes. This study compares the infectious disease risk of whole blood (WB) donors enrolled as therapeutic (T) donors to voluntary WB donors to evaluate the safety of blood products provided by the T donors. STUDY DESIGN AND METHODS This was a retrospective cohort study of all WB donations at the Australian Red Cross Blood Service who donated between January 1, 2011, and December 31, 2013, comparing a yearly mean of 11,789 T donors with 107,773 total donations and a yearly mean of 468,889 voluntary WB donors with 2,584,705 total donations. We compared postdonation notification of infectious illnesses, bacterial contamination screening results, and positive tests for blood borne viruses in T and WB donors. RESULTS Rates of transfusion-transmissible infections in donations destined for component manufacture were significantly lower in therapeutic donations compared to voluntary donations (8.4 vs. 21.6 per 100,000 donations). Bacterial contamination (43.0 vs. 45.9 per 100,000 donations) and postdonation illness reporting (136.2 vs. 110.8 per 100,000 donations) were similar in both cohorts. CONCLUSIONS The Australian therapeutic venisection program enables T donors to provide a safe and acceptable source of donated WB that has a low infectious disease risk profile.
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- 2016
8. Re-evaluating the residual risk of transfusion-transmitted Ross River virus infection
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Anthony J. Keller, Philip Kiely, Clive R. Seed, Joanne Pink, Veronica C. Hoad, and Helen M. Faddy
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Ross river virus infection ,viruses ,Population ,Blood Donors ,030204 cardiovascular system & hematology ,medicine.disease_cause ,Risk Assessment ,complex mixtures ,03 medical and health sciences ,Ross River virus ,0302 clinical medicine ,medicine ,Humans ,Blood Transfusion ,030212 general & internal medicine ,Chikungunya ,Alphavirus infection ,education ,education.field_of_study ,biology ,Alphavirus Infections ,Risk of infection ,Australia ,virus diseases ,Hematology ,General Medicine ,biology.organism_classification ,medicine.disease ,Virology ,Residual risk ,Risk assessment ,Demography - Abstract
Background and objectives Ross River virus (RRV) is an enveloped, RNA alphavirus in the same antigenic group as chikungunya virus. Australia records an annual average of 5000 laboratory-confirmed RRV infections. While RRV is currently geographically restricted to the Western Pacific, the capacity of arboviruses for rapid expansion is well established. The first case of RRV transfusion-transmission was recently described prompting a comprehensive risk assessment. Materials and methods To estimate the RRV residual risk, we applied laboratory-confirmed RRV notifications to two published models. This modelling generated point estimates for the risk of viraemia in the donor population, the risk of collecting a viraemic donation and the predicted number of infected components. Results The EUFRAT model estimated the risk of infection in donors as one in 95 039 (one in 311 328 to one in 32 399) to one in 14 943 (one in 48 593 to one in 5094). The point estimate for collecting a RRV viraemic donation varied from one in 166 486 (one in 659 078 to one in 49 158) (annualized national risk) to one in 26 117 (one in 103 628 to one in 7729) (area of high transmission). The modelling predicted 8–11 RRV-infected labile blood components issued in Australia during a 1-year period. Conclusion Considering the uncertainty in the modelled estimates, the unknown rate of RRV donor viraemia and the low severity of any recipient RRV infection, additional risk management for RRV in Australia will initially be restricted to strengthening the messaging to donors regarding prompt reporting of any postdonation illnesses.
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- 2016
9. Hepatitis E
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Juraj Petrik, Miguel Lozano, Clive R. Seed, Helen M. Faddy, Anthony J. Keller, Patricia Santos Prado Scuracchio, Silvano Wendel, Anton Andonov, Margaret Fearon, Gilles Delage, Jun Zhang, James Wai Kuo Shih, Pierre Gallian, Rachid Djoudi, Pierre Tiberghien, Jacques Izopet, Jens Dreier, Tanja Vollmer, Cornelius Knabbe, Rakesh Aggarwal, Amit Goel, Anna Rita Ciccaglione, Keiji Matsubayashi, Masahiro Satake, Kenji Tadokoro, Sook-Hyang Jeong, Hans L. Zaaijer, Eugene Zhiburt, Jason Chay, Diana Teo, Sze Sze Chua, Maria Piron, Silvia Sauleda, José-Manuel Echevarría, Harry Dalton, Susan L. Stramer, Scottish National Blood Transfusion Service, Department of Hemotherapy and Hemostasis, University Clinical Hospital, School of Environmental and Life Sciences, Newcastle University [Newcastle], Institute for Study of Earth, Oceans and Space, University of New Hampshire (UNH), Laboratory of Microbiology, Wageningen University and Research [Wageningen] (WUR), Etablissement Français du Sang - Alpes-Méditerranée (EFS - Alpes-Méditerranée), Etablissement Français du Sang, Emergence des Pathologies Virales (EPV), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Assistance Publique - Hôpitaux de Marseille (APHM)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Hospitalier Universitaire Méditerranée Infection (IHU Marseille), Interactions hôte-greffon-tumeur, ingénierie cellulaire et génique - UFC (UMR INSERM 1098) (RIGHT), Institut National de la Santé et de la Recherche Médicale (INSERM)-Etablissement français du sang [Bourgogne-Franche-Comté] (EFS BFC)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Department of Laboratory Medicine and Molecular Diagnostics, Robert-Bosch-Hospital, Centro Nacional de Microbiología, Instituto de Salud Carlos III [Madrid] (ISC), European Centre for Environment and Human Health, Royal Cornwall Hospital-Plymouth University, Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Etablissement français du sang [Bourgogne-Franche-Comté] (EFS [Bourgogne-Franche-Comté]), Royal Cornwall Hospital-Universities of Exeter and Plymouth, Landsteiner Laboratory, Medical Microbiology and Infection Prevention, AII - Infectious diseases, and AII - Amsterdam institute for Infection and Immunity
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[SDV]Life Sciences [q-bio] ,Hematology ,General Medicine ,ComputingMilieux_MISCELLANEOUS - Abstract
International audience
- Published
- 2015
10. The residual risk of transfusion-transmitted cytomegalovirus infection associated with leucodepleted blood components
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Joanne Pink, Janet Yuen Ha Wong, Helen M. Faddy, Anthony J. Keller, Mark N. Polizzotto, and Clive R. Seed
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Blood Platelets ,Risk ,Erythrocytes ,Congenital cytomegalovirus infection ,Cytomegalovirus ,Blood Component Transfusion ,Blood Donors ,Asymptomatic ,Serology ,Mice ,Leukocytes ,Animals ,Humans ,Medicine ,Red Cell ,biology ,business.industry ,Transmission (medicine) ,Hematology ,General Medicine ,medicine.disease ,Residual risk ,Risk Estimate ,Cytomegalovirus Infections ,Immunology ,biology.protein ,Antibody ,medicine.symptom ,business - Abstract
Background and Objectives Cytomegalovirus poses a risk to transfusion safety as its transmission to an immunocompromised recipient may lead to significant clinical sequelae. Once infection is established, it is lifelong and generally asymptomatic. Strategies to reduce the risk of transfusion-transmitted CMV (TT-CMV) include donor serological testing and blood component leucodepletion to deplete the transmissible reservoir. We estimate the residual risk for non-CMV antibody screened, leucodepleted (LD-only) fresh blood components. Materials and Methods We established an approach to estimate the risk of TT-CMV under various scenarios. We estimated the probability of an infectious component, for both red cells and platelets, as a function of the observed WBC filter failure rate and the probability that such a unit was also contaminated with infectious virus. Results Using this model, the estimated combined residual risk of LD-only red cell and platelet units was very low, 1 in 13 575 000 (95%CI:1 in 1 344 167 000–1 in 1 730 000) as was the individual residual risk estimate for LD-only red cells, 1 in 7 790 000 (95%CI: 1 in 771 307 000–1 in 993 000) and LD-only platelets, where a zero risk was estimated (95%CI: 0–1 in 1 074 000). Conclusion We describe a novel approach to assess the residual risk of LD-only components. This can be applied generally using local data. Our risk estimate for LD-only blood components in Australia is below the threshold of 1 in 1 million, generally considered negligible. This provides a useful indicator of the relative safety of LD-only components to assist clinical decisions when serologically screened inventory is unavailable.
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- 2015
11. Hepatitis E virus: do locally acquired infections in Australia necessitate laboratory testing in acute hepatitis patients with no overseas travel history?
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Robert L. Flower, Clive R. Seed, Ashish C. Shrestha, Helen M. Faddy, and Anthony J. Keller
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traveller ,medicine.medical_specialty ,Swine ,viruses ,Developing country ,Review ,hepatitis E virus ,Disease ,medicine.disease_cause ,Pathology and Forensic Medicine ,Hepatitis E virus ,Zoonoses ,Environmental health ,Animals ,Humans ,Seroprevalence ,Medicine ,Acute hepatitis ,zoonotic ,seroprevalence ,business.industry ,Transmission (medicine) ,Public health ,Australia ,virus diseases ,Outbreak ,Hepatitis E ,medicine.disease ,Virology ,laboratory testing ,chronic hepatitis ,business - Abstract
Summary Hepatitis E virus (HEV) is emerging as a global public health threat. Water-borne HEV outbreaks are common in developing countries and are associated with genotypes 1 and 2. In industrialised countries, sporadic cases of zoonotic transmission associated with genotypes 3 and 4 are increasingly being reported. Transfusion- and transplantation-transmitted HEV have been documented, although ingestion of contaminated food is thought to be the major transmission route. Severe disease is possible and chronic hepatitis infection occurs in solid-organ-transplant recipients and in patients with immunosuppressive disorders. In Australia, HEV cases are mainly travellers returning from disease endemic countries. Indeed, there are few reported cases of locally acquired HEV. Pigs in Australia have been shown to be infected with HEV, which indicates the possibility of zoonotic transmission. The extent of locally acquired infection is not known, however it may be greater than expected and may necessitate laboratory testing in patients reporting no overseas travel.
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- 2015
12. Bacterial testing of platelets - has it prevented transfusion-transmitted bacterial infections in Australia?
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George Kotsiou, J. Thyer, Peta M Dennington, Joanne Pink, Barbara Bell, Z. Perkowska‐Guse, Hiu Tat Chan, S. Ismay, and Anthony J. Keller
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Blood Platelets ,medicine.medical_specialty ,Propionibacterium ,Blood Safety ,Platelet Transfusion ,030204 cardiovascular system & hematology ,Bacterial growth ,Biology ,medicine.disease_cause ,Sepsis ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,Culture Techniques ,medicine ,Humans ,Platelet ,Incidence (epidemiology) ,Incidence ,Australia ,Pathogenic bacteria ,Hematology ,General Medicine ,Bacterial Infections ,biology.organism_classification ,medicine.disease ,Platelet transfusion ,Immunology ,Anaerobic exercise ,030215 immunology - Abstract
Background and Objectives Australia introduced bacterial contamination screening (BCS) for platelet components in April 2008. This study presents analysis performed to assess the efficacy of testing. Materials and Methods Seven-day aerobic and anaerobic culture is performed using the BacT/ALERT 3D system. Following an initial machine positive (IMP) flag, all associated components are recalled, and/or clinicians treating already transfused patients are notified. IMPs are categorized as ‘machine false positive’, ‘confirmed positive’ or ‘indeterminate’ depending on culture results of initial and repeat samples. Results Between 2010 and 2012, 1·1% of platelet donations tested IMP; since 2013, this rate has fallen to 0·6% through improved instrument management, reducing false-positive IMPs but maintaining sensitivity for cultures yielding bacterial growth. On average, 66% of confirmed positive and indeterminate platelet units had been transfused at the time of detection. The majority (95%) of these grew Propionibacterium sp., a slow-growing organism that rarely causes sepsis in the transfusion setting. The incidence of reported transfuion-transmitted bacterial infection (TTBI) has fallen since the introduction of BCS, with a 4·2-fold [0·5, 28·2] lower rate from platelets. Conclusion BCS has been successful in detecting platelet units containing pathogenic bacteria. The incidence of TTBI from platelets has fallen since the introduction of BCS, but the risk has not been eliminated due to rare false-negative results. In the absence of a pathogen inactivation system for red blood cells, BCS provides ‘surrogate’ testing of red blood cells from which platelets have been manufactured.
- Published
- 2017
13. Postdonation iron replacement for maintaining iron stores in female whole blood donors in routine donor practice: results of two feasibility studies in Australia
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Sant-Rayn, Pasricha, Denese C, Marks, Hannah, Salvin, Tania, Brama, Anthony J, Keller, Joanne, Pink, and Joanna, Speedy
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Adult ,Young Adult ,Adolescent ,Iron ,Surveys and Questionnaires ,Dietary Supplements ,Ferritins ,Australia ,Feasibility Studies ,Humans ,Blood Donors ,Middle Aged - Abstract
Iron deficiency represents a risk to donor health and the blood supply. Efficacy trials indicate that postdonation iron replacement improves iron stores but they do not account for complexities of implementation in the routine collection context. We therefore conducted two prospective feasibility studies in Australian donor centers.In both studies we recruited female donors between 18 and 45 years who had made at least one donation in the previous 12 months. In READ (replacement advice), female donors were given a recommendation to self-procure postdonation iron. In DIRECT (donor iron replacement), donors were provided with a course of iron supplements. Donors could return to donate at their discretion and were surveyed after the recruitment visit and again toward the end of the 13-month follow-up. Donor uptake, adverse effects, effectiveness in maintaining iron stores, and workflow impact were assessed.We recruited 1404 (70.9% of invited) donors to READ and 768 (53.2% of invited) to DIRECT. READ and DIRECT extended predonation interviews by 1 and 5 minutes, respectively. Among participants, 44 and 88% took iron in READ and DIRECT, respectively. Adverse effects were common but usually mild. READ failed to maintain iron stores in the population, but was effective in donors who consumed more than 75% of the recommended dose. DIRECT was effective in preventing declines in ferritin concentration.Trade-offs between cost, complexity, uptake, and effectiveness must be considered in the implementation of postdonation iron supplementation.
- Published
- 2017
14. A refined method for estimating the size of the potential blood donor pool in Australia
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Claire E. Styles, Tarana T.A. Lucky, Joanne Pink, David Wilson, Clive R. Seed, Anthony J. Keller, and June Lee
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education.field_of_study ,business.industry ,Immunology ,Population ,Hematology ,Total population ,3. Good health ,Blood donor ,Australian population ,Immunology and Allergy ,Medicine ,Potential donor ,Blood supply ,Epidemiologic data ,education ,business ,Deferral ,Demography - Abstract
Background To be eligible to donate blood, potential donors must meet certain eligibility criteria to ensure safety to the donor and to the blood supply. In Australia, there is no reliable estimate of the size of the donor-eligible population. This study uses a refinement to a published method to determine the population prevalence of donor-exclusion factors and subsequently estimates the size of the potential donor pool in Australia. Study Design and Methods A total of 70 donor-exclusion factors (in addition to age) were identified. The donor-eligible population was estimated by subtracting the prevalence of the exclusion factors from the total population. Prevalence of the donor-exclusion factors was adjusted for age, deferral period, and overlap of multiple conditions. Overlap was adjusted by extending a published random-probability model according to known association of epidemiologic data on overlapping conditions. Results The most prevalent (deferral period–adjusted) donor-exclusion factor among the 16- to 80-year-old Australian population was variant Creutzfeldt-Jakob disease–related travel risk (6.8%) followed by upper respiratory tract infections (6.4%). After exclusion of all factors, and accounting for overlapping factors, 62% of 16- to 80-year-olds or 47.3% of the total population were donor eligible in Australia. Conclusion We developed a refined method for estimating the size of the donor-eligible population. Applying this method to Australia, we estimate that approximately 10.7 million people (62% of the 16- to 80-year-olds) were eligible to donate blood in Australia in 2012.
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- 2014
15. Understanding noncompliance with selective donor deferral criteria for high-risk behaviors in Australian blood donors
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Clive R. Seed, June F. Lee, Tarana T.A. Lucky, Ann McDonald, Daniel Waller, Handan Wand, Glen Shuttleworth, Anthony J. Keller, David Wilson, Stephen Wroth, and Joanne Pink
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medicine.medical_specialty ,education.field_of_study ,business.industry ,Immunology ,Population ,Risk behavior ,Hematology ,Odds ratio ,Donor deferral ,Confidence interval ,Surgery ,Residual risk ,Screening questionnaire ,medicine ,Immunology and Allergy ,business ,education ,Demography ,Sex work - Abstract
Background Using a predonation screening questionnaire, potential blood donors are screened for medical or behavioral factors associated with an increased risk for transfusion-transmissible infection. After disclosure of these risks, potential donors are deferred from donating. Understanding the degree of failure to disclose full and truthful information (termed noncompliance) is important to determine and minimize residual risk. This study estimates the prevalence of, and likely reasons for, noncompliance among Australian donors with the deferrals for injecting drug use, sex with an injecting drug user, male-to-male sex, sex worker activity or contact, and sex with a partner from a high-HIV-prevalence country. Study Design and Methods An anonymous, online survey of a nationally representative sample of Australian blood donors was conducted. Prevalence of noncompliance with deferrable risk categories was estimated. Factors associated with noncompliance were determined using unadjusted and adjusted odds ratios. Results Of 98,044 invited donors, 30,790 donors completed the survey. The estimated prevalence of overall noncompliance (i.e., to at least one screening question) was 1.65% (95% confidence interval CI, 1.51%-1.8%). Noncompliance with individual deferrals ranged from 0.05% (sex work) to 0.54% (sex with an injecting drug user). The prevalences of the disclosed exclusionary risk behaviors were three to 14 times lower than their estimated prevalence in the general population. Conclusion The prevalence of noncompliance is relatively low but our estimate is likely to be a lower bound. The selected high-risk behaviors were substantially less common in blood donors compared to the general population suggesting that self-deferral is effective. Nevertheless, a focus on further minimization should improve the blood safety.
- Published
- 2014
16. An 8-week course of 45 mg of carbonyl iron daily reduces iron deficiency in female whole blood donors aged 18 to 45 years: results of a prospective randomized controlled trial
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Tania Brama, Anthony J. Keller, Denese C. Marks, Phillip Mondy, Kathryn Robinson, Joanna Speedy, and Hugh Capper
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medicine.medical_specialty ,biology ,business.industry ,Immunology ,Placebo-controlled study ,Hematology ,Iron deficiency ,Direct reduced iron ,Placebo ,medicine.disease ,Gastroenterology ,Surgery ,Ferritin ,Carbonyl iron ,Internal medicine ,biology.protein ,Immunology and Allergy ,Medicine ,Hemoglobin ,business ,Whole blood - Abstract
Background Blood donation is known to contribute to iron deficiency in regular blood donors. This study investigated the safety and efficacy of postdonation iron replacement to mitigate iron deficiency in blood donors. Study Design and Methods A total of 282 female whole blood donors aged 18 to 45 were prospectively randomized in a double-blinded placebo controlled trial to receive an 8-week postdonation course of carbonyl iron (45 mg daily) or placebo. The primary endpoint was prevalence of iron deficiency (ferritin < 15 ng/mL) at 12 weeks postdonation. Secondary endpoints were eligibility to donate based on capillary hemoglobin (Hb) and incidence of gastrointestinal (GI) complaints. Results Ferritin levels at Week 12 were significantly higher in donors receiving carbonyl iron (17.0 ± 10.9 ng/mL) compared with those receiving placebo (10.6 ± 8.4 ng/mL; p
- Published
- 2013
17. Trends in transfusion-transmissible infections among Australian blood donors from 2005 to 2010
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S. Ismay, Anthony J. Keller, Handan Wand, Clive R. Seed, Ann McDonald, Tarana T.A. Lucky, June Lee, and David Wilson
- Subjects
education.field_of_study ,medicine.medical_specialty ,Multivariate analysis ,business.industry ,Donor selection ,Incidence (epidemiology) ,Immunology ,Population ,Hematology ,Rate ratio ,medicine.disease ,Surgery ,symbols.namesake ,Environmental health ,symbols ,Immunology and Allergy ,Medicine ,Syphilis ,Poisson regression ,Risk factor ,business ,education - Abstract
Background Routine monitoring of trends in transfusion-transmissible infections (TTIs) is essential to maintaining and improving transfusion safety. Although periodic studies have been published there is no comprehensive trend analysis for TTIs in Australian donors. This study determined recent trends in TTIs for which testing is conducted in Australia and described key attributes of infected blood donors. Study Design and Methods This is a retrospective analysis using data on donation testing for TTIs (2005-2010) from the national blood service donor database and data on postdonation interviews with TTI-positive donors (2008-2010) from a risk factor database incorporating responses to standardized interview questions. The study measured the prevalence and incidence of TTIs in Australia and assessed their time trends. Multivariate analysis of time trends was conducted using Poisson regression models. Results Overall, the prevalence and incidence of TTIs in 2005 to 2010 remained low and steady. The prevalence of hepatitis C virus decreased (rate ratio [RR], 0.93; 95% confidence interval [CI], 0.89-0.97) and the prevalence of active syphilis increased (RR, 1.51; 95% CI, 1.15-1.99) significantly during the study period. Prevalence of TTIs among Australian blood donors was substantially lower than that in the general population and no unique risk factors were identified in test-positive blood donors when compared with the general population. Conclusion Both the prevalence and the incidence of TTIs in Australian blood donors remained low, with a steady or declining trend for most infections except active syphilis. The lower prevalence of TTIs in blood donors compared with the general population reflects the effectiveness of donor education and donor selection measures in Australia.
- Published
- 2013
18. Refining the risk estimate for transfusion-transmission of occult hepatitis B virus
- Author
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Veronica C. Hoad, Philip Kiely, Anthony J. Keller, and Clive R. Seed
- Subjects
Risk ,medicine.medical_specialty ,Hepatitis B virus ,Population ,Blood Donors ,Transfusion transmission ,030204 cardiovascular system & hematology ,medicine.disease_cause ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,Epidemiology ,medicine ,Humans ,Blood Transfusion ,030212 general & internal medicine ,Hepatitis B Antibodies ,education ,Oligonucleotide Array Sequence Analysis ,education.field_of_study ,business.industry ,Hematology ,General Medicine ,Models, Theoretical ,Hepatitis B ,Occult ,Hepatitis B Core Antigens ,Surgery ,Residual risk ,Risk Estimate ,DNA, Viral ,business ,Risk assessment - Abstract
Background and Objectives We previously published a model to estimate the residual risk (RR) for occult hepatitis B infection (OBI) in the absence of universal anti-HBc testing. To incorporate new information on the epidemiology of OBI, we describe model refinements and estimate a more accurate HBV RR due to OBI in Australia. Materials and Methods In our original model, the OBI risk, p(OBI), was defined by the rate of ‘non-detection’ by the HBV DNA screening test in use, p(NAT non-detection), and the average infectivity of blood components from OBI donors, p(transmission). We revised the model by integrating three refinements: that donations with anti-HBs levels of >10 IU/l, or donations solely for manufactured plasma products, be excluded from the risk calculation, and an updated estimate of p(transmission). Results Refining our OBI RR model resulted in a more than 10-fold reduction in the reported RR risk to recipients from OBI in our donor population. Based on the use of a common data set, the mean OBI RR risk decreased from 1 in 374 354 donations (95% CI: 1 in 191 940–1 072 681) to 1 in 3 984 033 (95% CI: 1 in 1 146 188–65 268 257) for the refined model. Conclusion Our model refinements provide a more realistic measure of the HBV RR in the donor population. Unlike the previous model, the new model demonstrates that the risk of HBV due to OBI in the Australian blood donor population is negligible, and further potentially cost-ineffective risk management strategies are not currently warranted.
- Published
- 2016
19. Hepatitis E virus RNA in Australian blood donations
- Author
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Ashish C, Shrestha, Robert L P, Flower, Clive R, Seed, Anthony J, Keller, Robert, Harley, Hiu-Tat, Chan, Veronica, Hoad, David, Warrilow, Judith, Northill, Jerry A, Holmberg, and Helen M, Faddy
- Subjects
Seroepidemiologic Studies ,Blood Safety ,Australia ,Hepatitis E virus ,Humans ,RNA, Viral ,Transfusion Reaction ,Blood Donors ,Viral Load ,Polymerase Chain Reaction ,Phylogeny ,Hepatitis E - Abstract
Hepatitis E virus (HEV) poses a risk to transfusion safety. In Australia, locally acquired HEV is rare and cases are mainly reported in travelers returning from countries endemic for HEV. The risk posed by HEV to transfusion safety in Australia is unknown; therefore, we aimed to measure the rate of current HEV infection in Australian blood donations.A total of 14,799 blood donations were tested for HEV RNA by transcription-mediated amplification, with confirmatory testing by reverse transcription-polymerase chain reaction. Viral load quantification and phylogenetic analysis was performed on HEV RNA-positive samples.One (0.0068%; 95% confidence interval [CI], 0.0002%-0.0376%) sample was confirmed positive for HEV RNA, resulting in a risk of collecting a HEV-viremic donation of 1 in 14,799 (95% CI, 1 in 584,530 to 1 in 2,657). The viral load in this sample was approximately 15,000 IU/mL, and it was determined to be Genotype 3.Our finding of 1 in 14,799 Australian donations positive for HEV RNA is lower than that from many other developed countries; this is consistent with the relatively low seroprevalence in Australia. As this HEV RNA-positive sample was Genotype 3, it seems likely that this infection was acquired through zoonotic transmission, either within Australia or overseas in a developed nation. HEV has the potential to pose a risk to transfusion safety in Australia; however, additional, larger studies are required to quantify the magnitude of this risk.
- Published
- 2016
20. Measures to prevent transfusion-related acute lung injury (TRALI)
- Author
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Miodrag Palfi, K. Qian, L. McLaughlin, Héctor Baptista-González, Anthony J. Keller, A. Guerra-Márquez, Kevin J Land, C. K. Lin, Joanne Pink, E. Massey, C. Chapman, P. Tiberghien, P. Turek, N. Greppi, J. Lee, K. Maslanka, Simon Panzer, S. A. Sánchez-Guerrero, Anna Green, J. Y. Muller, L. Williamson, E. Lachert, G. Semana, Daniel R. Ambruso, C. Martínez-Murillo, Peter Tomasulo, N. Win, Laura Porretti, Steve Kleinman, Mindy Goldman, Anneke Brand, R. Holdsworth, Henk W. Reesink, Angelika Reil, Philip J. Norris, P. Dunn, Jürgen Bux, Tom Krusius, D. Legrand, Rutger A. Middelburg, Beat M. Frey, Christopher C. Silliman, D. Dinesh, H. Daly, Richard J. Benjamin, Y. Meng, H. Okazaki, Eduardo Muñiz-Diaz, Tanya Petraszko, Neil Blumberg, E. B. Zhiburt, E. Juvonen, Gösta Berlin, Peta M Dennington, J. Sun, J. Dagger, Johanna C. Wiersum-Osselton, E. McSweeney, L. Puig Rovira, Małgorzata Uhrynowska, V. Rehacek, Magdalena Letowska, C. van Tilburg, Ewa Brojer, O. C. Illoh, H. Schennach, R. J. Davey, H. Rodriguez-Moyado, E. Castro, D. J. Chaffin, Anne F. Eder, and Paolo Rebulla
- Subjects
medicine.medical_specialty ,Psychoanalysis ,Philosophy ,medicine ,Hematology ,General Medicine ,medicine.disease ,Intensive care medicine ,Transfusion-related acute lung injury - Abstract
H. W. Reesink, J. Lee, A. Keller, P. Dennington, J. Pink, R. Holdsworth, H. Schennach, M. Goldman, T. Petraszko, J. Sun, Y. Meng, K. Qian, V. Rehacek, P. Turek, T. Krusius, E. Juvonen, P. Tiberghien, D. Legrand, G. Semana, J. Y. Muller, J. Bux, A. Reil, C. K. Lin, H. Daly, E. McSweeney, L. Porretti, N. Greppi, P. Rebulla, H. Okazaki, S. A. Sanchez-Guerrero, H. A. Baptista-Gonzalez, C. Martinez-Murillo, A. Guerra-Marquez, H. Rodriguez-Moyado, R. A. Middelburg, J. C. Wiersum-Osselton, A. Brand, C. van Tilburg, D. Dinesh, J. Dagger, P. Dunn, E. Brojer, M. Letowska, K. Maslanka, E. Lachert, M. Uhrynowska, E. Zhiburt, M. Palfi, G. Berlin, B. M. Frey, L. Puig Rovira, E. Muniz-Diaz, E. Castro, C. Chapman, A. Green, E. Massey, N. Win, L. Williamson, C. C. Silliman, D. J. Chaffin, D. R. Ambruso, N. Blumberg, P. Tomasulo, K. J. Land, P. J. Norris, O. C. Illoh, R. J. Davey, R. J. Benjamin, A. F. Eder, L. McLaughlin, S. Kleinman & S. Panzer
- Published
- 2012
21. A prospective trial assessing the safety and efficacy of collecting up to 840 mL of plasma in conjunction with saline infusion during plasmapheresis
- Author
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Samantha Reid, Caro‐Anne Badcock, Mark Buzza, Denese C. Marks, June Lee, Elisa Cassin, Tanja Hartkopf-Theis, M. Kwok, Hugh Capper, Hung Yang, Anthony J. Keller, and Carolyn Corrigan
- Subjects
biology ,business.industry ,medicine.medical_treatment ,Saline infusion ,Immunology ,Hematology ,Crossover study ,Blood proteins ,Anesthesia ,medicine ,biology.protein ,Immunology and Allergy ,Plasmapheresis ,Antibody ,Adverse effect ,Prospective cohort study ,business ,Saline - Abstract
BACKGROUND: The demand for plasma for manufacturing intravenous immunoglobulin and other plasma derivatives is increasing. A prospective study was conducted to determine whether up to 840 mL of plasma could be safely and effectively collected in conjunction with saline infusion during plasmapheresis. STUDY DESIGN AND METHODS: Ninety-one plasma donors were enrolled in a modified 3 × 3 crossover study to assess the equivalence of three plasma collection methods: 750 mL of plasma with no saline (control, Method 1), 840 mL of plasma with a 250-mL saline infusion during and at the end of the donation (Method 2), and 800 mL of plasma with a 500-mL saline infusion at the end of the donation (Method 3). The primary efficacy endpoint was the total protein concentration of the collected plasma. Secondary efficacy endpoints were immunoglobulin (Ig)G and Factor (F)VIII plasma concentration and donors' acceptance of the new procedures. Safety was determined from the adverse event (AE) rate. RESULTS: The total protein, IgG, and FVIII concentrations in plasma collected under Methods 2 and 3 were significantly lower than those in plasma collected under Method 1 (p
- Published
- 2012
22. The challenges of managing donor haemoglobin
- Author
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Anthony J. Keller, M. Bower, Denese C. Marks, Sandra Minck, and Joanna Speedy
- Subjects
medicine.medical_specialty ,education.field_of_study ,biology ,business.industry ,Population ,Confounding ,Iron deficiency ,medicine.disease ,Ferritin ,Clinical research ,Donation ,biology.protein ,Medicine ,Operations management ,business ,Intensive care medicine ,education ,Finger prick ,Point of care - Abstract
Haemoglobin screening has long provided blood services with a means to determine the eligibility of prospective donors. Screening aims to protect donors from donating whilst anaemic, minimise the risk of anaemia developing as a result of donation and ensure an adequate red cell component for the transfusion recipient. Addressing the limitations, risks and benefits of haemoglobin screening to both donor and recipients requires continuous surveillance and an evidence-based response to evolving clinical research and technological innovations. Challenges include determination of appropriate thresholds in specific populations, management of donors with low haemoglobin, realising the limitations of haemoglobin as a predictor of iron status, and the assessment and selection of alternative screening devices. Current haemoglobin thresholds are now in doubt, with recent large US population studies re-defining normal ranges for haemoglobin. A further confounding factor is local and international research highlighting the significant prevalence of iron deficiency in eligible donor populations. This reinforces the limitations of haemoglobin as a stand-alone point of care screening test. Current practice at the Australian Red Cross Blood Service (Blood Service) to assess donor haemoglobin involves a pre-donation capillary finger prick sample analysed on the HemoCue® 201+. Minimum thresholds for whole blood donation are 120 and 130 g/l for females and males respectively. Donors with low haemoglobin are deferred, offered ferritin testing, and referred to their GP for management if subsequently identified as anaemic and/or iron deficient. The management of low haemoglobin deferrals is currently under review. These account for 16% of all deferrals. Routine ferritin testing of low haemoglobin-related deferrals indicates 75% are associated with iron deficiency, with approximately 60% in females aged ≤50 years. Local research indicates a substantial loss of donations due to delayed or non-return following a low haemoglobin deferral. Additional local research suggests that screening with haemoglobin and iron indices enables prediction of donors at risk of subsequent anaemia and who would most benefit from prevention strategies. Technological advances may allow additional means by which to improve donor experience and safety. These include the use of a more palatable non-invasive haemoglobin analyser, and point of care ferritin testing, both of which are to be assessed by the Blood Service for accuracy and feasibility of implementation. The greatest challenge is to manage the balance of donor health and well-being with the potential supply loss from healthy donors. To optimise donor iron status and subsequently improve efficiency and supply through reduced deferrals and improved return rates, the Blood Service is considering a comprehensive targeted strategy. Options include improving informed decision-making for donors, education, ferritin screening, donation frequency modification and/or iron supplementation. Our recently completed randomised, double blind trial of carbonyl iron supplementation in premenopausal female blood donors will be a valuable resource in the development of this strategy. Initiatives need to be responsive to the needs and expectations of donors, community and health professionals, whilst considering the immediate and long-term impacts on inventory and logistics of implementation. The Blood Service has established a cross-functional Iron Taskforce to scope and develop such strategies.
- Published
- 2011
23. Deferral of males who had sex with other males
- Author
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S. Wendel, Clive R. Seed, Yanyan Zhu, L. S. McLaughlin, B. Danic, R. Kullaste, Aleksandra Rosiek, Huan Yang, Richard J. Benjamin, Claudio Velati, Josiane Pillonel, P. Turek, F. M. Moftah, Ju Whei Lee, C. K. Lin, Simon Panzer, R. N. Makroo, Giuliano Grazzini, Erhard Seifried, E. Zhiburt, V. T. Giner, Peter Flanagan, Ryszard Pogłód, A. D’Artote González, Dana V. Devine, Bengt Ekermo, Mindy Goldman, Joseph Murray, Anthony J. Keller, R. W. Olaussen, Simonetta Pupella, P. Rozman, Markus M. Mueller, G Folléa, Polonca Mali, S. Biagini, Anne F. Eder, F. Bigey, A. Bravo Lindoro, H. W. Reesink, E. Castro Izaguirre, Celso Bianco, S. Gulube, Magdalena Letowska, S. M. Barnes, and Kenji Tadokoro
- Subjects
education.field_of_study ,business.industry ,Donor selection ,Status quo ,media_common.quotation_subject ,Risk of infection ,Population ,Hematology ,General Medicine ,medicine.disease ,Acquired immunodeficiency syndrome (AIDS) ,Immunology ,Medicine ,Homosexuality ,Seroconversion ,business ,Deferral ,education ,Demography ,media_common - Abstract
Donor history questionnaires for the determination of blood donor eligibility are a critical layer of blood safety. Early in the course of the AIDS epidemic in North America homosexual men with multiple partners were identified as one of the segments of the population with the highest risk of infection. Voluntary deferral of this group from blood donation led to a dramatic decrease in transfusion-transmitted HIV even before testing was introduced. In the early 1980s blood donors were deferred in England, the US and other nations, if they were ‘homosexual males with multiple partners’. After the implementation of HIV testing in 1985, the majority of the HIV-positive donors identified revealed ‘men having sex with men’ (MSM) behavior, leading the US Food and Drug Administration (FDA) to recommend indefinite deferral of all men who ‘have had sex with men, even once since 1977’; many other regulators and jurisdictions have enacted similar criteria. Three decades later, despite the recognition of other modes of transmission, MSM donors are still among the population segments with the highest prevalence and incidence of HIV in countries around the world. No other donor eligibility criterion has generated as much controversy or public discourse [1,2]. Proponents for change point out that in many countries other key components of blood safety such as donor testing and blood center process control have improved vastly, reducing the contribution of donor questioning to safety. Gay advocates in particular argue that donor selection policies based on MSM are discriminatory against gay and bisexual men in that they amount to a de facto permanent exclusion on the grounds of sexual preference, and are unfair, as other groups with similar risks of HIV infection are allowed to donate blood after shorter time-period deferrals designed to cover the seroconversion window. On the opposite side of the discussion, recipient advocacy groups and regulators are understandably adverse to any change that is not centered on improving safety. Recipient groups argue that they have suffered greatly due to transmission of HIV and HCV by transfusion, and they will be the bearers of any increase in risk that may result from policy changes. Because both MSM and recipients are vulnerable groups that have suffered in the past, the debate over possible changes in criteria has ethical, societal, and emotional dimensions not seen in discussions concerning other donor selection criteria. Of particular concern to blood operators is the prospect that young eligible donors may be dissuaded from donating blood to institutions that are perceived to act in a discriminatory and unfair fashion. This International Forum seeks to describe approaches to this issue and challenges to the status quo, in a snapshot in time. Since it is extremely difficult to obtaindatatoevaluatethepossibleimpactofpolicy changes made to address concerns expressed by advocacy groups, comparison of international practice is particularly valuable, since we may learn from approaches implemented in other jurisdictions. We received responses from 24 respondents representing countries on six continents. In most, but not all, the MSMpolicy isdetermined atthe national level. The following questions were asked of the respondents
- Published
- 2011
24. Serum hepcidin as a diagnostic test of iron deficiency in premenopausal female blood donors
- Author
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Anthony J. Keller, Zoe McQuilten, Erica M. Wood, Elizabeta Nemeth, Sant-Rayn Pasricha, Mark Westerman, and Tomas Ganz
- Subjects
Adult ,medicine.medical_specialty ,Population ,Blood Donors ,Reference range ,Sensitivity and Specificity ,Hepcidins ,Reference Values ,Hepcidin ,Internal medicine ,medicine ,Humans ,education ,Soluble transferrin receptor ,Whole blood ,education.field_of_study ,biology ,Chemistry ,Original Articles ,Iron Deficiencies ,Hematology ,Iron deficiency ,Metabolism ,medicine.disease ,Ferritin ,Endocrinology ,Premenopause ,Immunology ,biology.protein ,Female ,Deficiency Diseases ,Blood Chemical Analysis ,Antimicrobial Cationic Peptides - Abstract
Background Currently used indicators of iron status have limitations. Hepcidin, a key regulator of iron metabolism, is reduced in iron deficiency. We sought to determine the properties of hepcidin as a diagnostic test of iron deficiency.Design and Methods Sera from female, non-anemic, whole blood donors were analyzed for hepcidin (enzyme-linked immunosorbent assay), ferritin, soluble transferrin receptor and C-reactive protein. Iron deficiency was defined as (i) serum ferritin less than 15 ng/mL or (ii) soluble transferrin receptor /log(ferritin) index greater than 3.2 if the C-reactive protein concentration was less than 10 mg/L, or greater than 2.2 if the C-reactive protein concentration was greater than 10 mg/L). Receiver operating characteristic curves were plotted to determine the overall utility and identify optimal cut-points of hepcidin as a test of iron deficiency.Results In 261 blood donors the prevalence of iron deficiency defined by ferritin concentration was 59/261 [22.6% (17.5, 27.7)], whereas defined by soluble transferrin receptor/log(ferritin) index it was 53/261 [20.4% (15.4, 25.2)]. The 95% reference range of hepcidin concentration in the iron-replete population was 8.2–199.7 ng/mL. The area under the receiver operating characteristic curve for hepcidin compared with ferritin concentration less than 15 ng/mL was 0.87 (0.82, 0.92), while that compared with the soluble transferrin receptor /log(ferritin) index was 0.89 (95% CI 0.84, 0.93). For a diagnosis of iron deficiency defined by the soluble transferrin receptor/log(ferritin) index, hepcidin less than 8 ng/mL had a sensitivity of 41.5% and a specificity of 97.6%, while hepcidin less than 18 ng/mL had a sensitivity of 79.2% and a specificity of 85.6%.Conclusions Serum hepcidin concentration may be a useful indicator of deficient iron stores. Further studies are required to evaluate the role of hepcidin in the diagnosis of iron deficiency in other groups of patients.
- Published
- 2011
25. Hepatitis E Virus and Implications for Blood Supply Safety, Australia
- Author
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Clive R. Seed, Ashish C. Shrestha, Robert Harley, Helen M. Faddy, Jerry A. Holmberg, Robert L. Flower, Hiu Tat Chan, Kelly Rooks, and Anthony J. Keller
- Subjects
Microbiology (medical) ,safety ,Blood transfusion ,Letter ,Epidemiology ,viruses ,medicine.medical_treatment ,Blood Safety ,lcsh:Medicine ,Blood Donors ,medicine.disease_cause ,lcsh:Infectious and parasitic diseases ,Hepatitis E virus ,Seroepidemiologic Studies ,Environmental health ,antibody ,medicine ,Seroprevalence ,Humans ,lcsh:RC109-216 ,Risk factor ,blood donor ,Letters to the Editor ,Hepatitis E Virus and Implications for Blood Supply Safety, Australia ,Hepatitis ,seroprevalence ,Donor selection ,business.industry ,lcsh:R ,transmission ,Australia ,virus diseases ,medicine.disease ,Hepatitis E ,blood supply ,digestive system diseases ,Infectious Diseases ,risk factor ,Immunology ,business ,Malaria - Abstract
To the Editor: Hepatitis E virus (HEV) is an emerging public health concern for industrialized countries (1). Although HEV infection has been associated with travel to countries where the virus is endemic, cases of autochthonous HEV are increasing (2). Detection of HEV RNA in blood donations in the United Kingdom, Germany, the Netherlands, Japan, and China and accumulating reports of HEV transmitted through blood transfusion highlight the potential risk this virus poses to blood supply safety (1–4). In Australia, where most HEV infections are associated with travel (5), an average of 25 HEV cases occurred each year during 1999–2013 (http://www9.health.gov.au/cda/source/rpt_3.cfm). HEV infection is nationally notifiable in Australia, but the presence of subclinical infections and the lack of recent seroprevalence studies have prevented the accurate estimation of HEV incidence and population exposure. Thus, we examined HEV seroprevalence in a cohort of Australian blood donors, assessed risk factors for exposure, and used the data to examine the effectiveness of current blood safety strategies for the management of HEV in Australia. Plasma samples (n = 3,237) were collected from donors during August–September 2013. Information on age, sex, state of residence, new/repeat donor status, and overseas travel disclosure was obtained. Details of any relevant blood donation deferral (malaria, diarrhea) applied on previous donation attempts were also collected. Application of a specific malaria deferral code is routine for donors disclosing travel to a malaria-endemic country, and a diarrhea deferral applies when a donor reports having had diarrhea (of viral or unknown cause) 1 week before any attempted donation. All samples were tested for HEV IgG by using the Wantai HEV-IgG ELISA (Beijing Wantai Biologic Pharmacy Enterprise Co., Ltd, Beijing, China). Positive samples were tested for HEV IgM by using the Wantai HEV-IgM ELISA and for HEV RNA by using a prototype transcription-mediated amplification assay (Hologic Inc., San Diego, CA, USA). Of 3,237 samples, 194 (5.99%) were positive for HEV IgG (95% CI 5.18–6.81). Compared with estimates from previous studies that used the Wantai ELISA (6–9), our estimate is comparable to those reported from Scotland (4.7%) and New Zealand (4.2%) but lower than those from the United States (18.8%) and southwestern France (52.5%). Considerable debate exists regarding the sensitivity and specificity of HEV detection methods (2,10); however, on the basis of studies in France and the United Kingdom (9,10), we believe that the measured seroprevalence in our study is accurate. Our findings showed an increased seroprevalence of HEV associated with previous malaria deferral, diarrhea deferral, and age (multivariate logistic regression) (Table), the latter of which is consistent with previous findings (9). IgG seropositivity was also higher (7.73%) in donors who had traveled to a malaria-endemic country. HEV is often endemic to malaria-endemic countries (http://wwwnc.cdc.gov/travel/yellowbook/2014); however, the HEV exposure status of travelers is unknown before departure, so the exact place of exposure cannot be determined. Furthermore, 3.37% of donors in our study had evidence of previous HEV exposure; these donors had not reported travel outside Australia, so they may have acquired HEV locally. Because subclinical HEV infection is possible, persons infected locally may not be identified by the current donor screening questionnaire and thus pose a potential risk to blood supply safety. Table HEV (IgG) seroprevalence, and risk factors for exposure, in Australian blood donors* Detection of HEV IgM in 4 (2.06%) of the 194 samples from IgG-positive donors indicates the donors had been recently exposed to HEV (95% CI 0.06–4.06). All 4 donors had traveled overseas; 3 reported travel to malaria-endemic countries. HEV RNA was not detected in any of the HEV IgG–positive samples. Although it is encouraging that HEV nucleic acid was not detected, the sample size is insufficient to accurately determine the true rate of HEV RNA carriage among donors in this study; a larger study is planned. Management strategies to safeguard the Australian blood supply against transfusion-transmitted HEV are based on donor selection guidelines. To identify donors with possible bacteremia/viremia, including HEV, blood donation staff members ask donors several medical, behavioral, and travel-based questions before donation. These include questions relating to general wellness, sex practices, gastric upset, diarrhea, abdominal pain, and vomiting within the previous week. In addition, for 4 months after a donor’s return from travel to a malaria-endemic country, donations are restricted to plasma for fractionation. Some protection against blood donations from HEV-infected persons may occur because HEV and malaria are co-endemic to many countries. Our findings showed a higher HEV seroprevalence among donors with prior malaria or diarrhea deferrals; thus, malaria- and diarrhea-related screening questions may reduce contributions from donors with travel-associated HEV infection. Our findings showed HEV exposure in travelers and nontravelers, suggesting the possibility of imported and locally acquired HEV in Australia. Prior HEV exposure was higher in donors who were temporarily excluded from donating blood on previous donation attempts, suggesting the current management strategy in Australia is partially effective in minimizing any risk of HEV transmission through blood transfusion. However, the presence of HEV IgG in donors who reported no overseas travel and/or no prior related deferrals, coupled with the knowledge that asymptomatic infection is possible, suggests that additional safety precautions may be warranted.
- Published
- 2014
26. The risk of dengue transmission by blood during a 2004 outbreak in Cairns, Australia
- Author
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Clive R. Seed, Philip Kiely, Anthony J. Keller, and Catherine A. Hyland
- Subjects
viruses ,Immunology ,Population ,Biology ,Dengue virus ,medicine.disease_cause ,Risk Assessment ,Arbovirus ,Dengue fever ,Dengue ,Environmental health ,medicine ,Humans ,Immunology and Allergy ,Risk factor ,education ,education.field_of_study ,Australia ,Transfusion Reaction ,virus diseases ,Outbreak ,Hematology ,Models, Theoretical ,medicine.disease ,biology.organism_classification ,Virology ,Flavivirus ,Risk assessment - Abstract
BACKGROUND: Dengue virus (DENV) is a Flavivirus transmitted by the Aedes mosquito. The related arbovirus, West Nile virus, has been shown to be transfusion transmitted, which, added to the four recorded dengue transfusion-associated cases, indicates that DENV is also transfusion transmitted. The purpose of this study was to assess the risk of transfusion-transmitted DENV during a 2004 outbreak in the Australian city of Cairns. STUDY DESIGN AND METHODS: A mathematical model was constructed to estimate the risk of transfusion-transmitted dengue. The model's central premise is that the transmission risk is proportional to the frequency of dengue-viremic donations and correlates with the incidence of asymptomatic dengue viremia among the population at large. RESULTS: The modeling predicted that the total number of DENV infections (clinical plus subclinical) among the population at large during the entire outbreak ranged from 156 to 569 with the epidemic peak occurring between February 8 and March 6, 2004. The overall transmission risk during the entire outbreak was estimated as 1 in 19,759 (range, 1 in 3404 to 75,486) peaking at 1 in 5968 (range 1 in 1028 to 22,800). CONCLUSION: By use of the most conservative estimates for key variables, the risk of collecting a viremic donation could have been as high as 1 in 1028 during the peak of the 2004 outbreak. The model can be used to determine transfusion transmission risk levels during DENV outbreaks and inform decisions as to when fresh component restriction measures are required to minimize transfusion transmission risk.
- Published
- 2009
27. Estimating the risk of blood donation associated with HIV risk behaviours
- Author
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Anthony J. Keller, John M. Kaldor, J. A. Musto, Clive R. Seed, and Matthew Law
- Subjects
Male ,medicine.medical_specialty ,Blood transfusion ,medicine.medical_treatment ,Human immunodeficiency virus (HIV) ,Blood Donors ,HIV Infections ,Window period ,Hiv risk ,medicine.disease_cause ,Risk Assessment ,Men who have sex with men ,Risk-Taking ,Risk Factors ,medicine ,Humans ,Gynecology ,business.industry ,Australia ,Absolute risk reduction ,HIV ,Hematology ,Models, Theoretical ,Blood donor ,Donation ,Female ,business ,Demography - Abstract
summary A blood donor questionnaire and declaration, with deferral of potential donors at a higher risk of blood-borne infections, was introduced in Australia in the mid-1980s to reduce the risk of donation of HIV-infected blood. However, the absolute risk of HIV transmission through blood donation from high-HIV-risk donors has not been estimated. This study presents a new method of assessing the risk posed to the blood supply by selected HIV risk behaviours. A model was developed to estimate the probability of blood donation during the window period for HIV infection. Five scenarios for blood donors were considered: (1) men who have sex with men (MSM), (2) men who have sex with women in Australia, (3) women who have sex with partners from countries with a high HIV prevalence, (4) men who have sex with commercial sex workers in Australia and (5) people injecting drugs used once in a year. Those estimated to be at highest risk of becoming infected and donating in the window period were MSM. Women who have sex with men from countries of high HIV prevalence are at greater risk than men who have sex with female sex workers from Australia. These three groups under current Australian guidelines are deferred from donating blood for 12 months. In Australia, a single episode of injecting drug use is associated with very low risk of HIV transmission. The model presented in this study can be used to assess the impact of selected individual risk behaviours on the safety of the blood supply.
- Published
- 2008
28. Detection of bacterial contamination of platelet concentrates
- Author
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Erica M. Wood, C. P. McDonald, J. T. Wilson, Roslyn Yomtovian, Mindy Goldman, D. de Korte, Ruby N.I. Pietersz, A. Assal, William G. Murphy, W. R. Mayr, P. Herve, M. Foley, G. Andeu, E. Cadden, Sandra Ramirez-Arcos, Mark E. Brecher, Jens Kjeldsen-Kragh, Michael Schmidt, A. Salami, James P. AuBuchon, Anthony J. Keller, P. Coakley, Pascal Morel, Masahiro Satake, G. Henn, Jørgen Georgsen, C. Doherty, C. P. Engelfriet, Erhard Seifried, S. Winzar, V. Bosnes, and Henk W. Reesink
- Subjects
business.industry ,Immunology ,Medicine ,Platelet ,Hematology ,General Medicine ,Contamination ,business ,Microbiology - Published
- 2007
29. International Forum: 1
- Author
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Tom Krusius, Angelo R. Margaritis, Silvia Sauleda, Clive R. Seed, J. Pineau, C. P. Engelfriet, Anthony J. Keller, Catherine A. Hyland, Claudio Velati, C. L. Van Der Poel, H. W. Reesink, O. Flesland, A. Zanetti, Michael P. Busch, Miguel A. Vesga, S. Levičnik‐Stezina, C. Politis, L. Muylle, H. T. M. Cuijpers, M. Strong, D. Sondag‐Thull, W.K. Roth, Stuart M. Brown, C. Neiderhauser, J. M. Hernandez, Syria Laperche, Hans Erik Heier, Joliette Coste, S. Pastila, G. Levy, Indira Hewlett, Eleftherios C. Vamvakas, Jay S. Epstein, R. Elgin, Susan L. Stramer, G. Henn, Bengt Ekermo, Josiane Pillonel, and C. K. Lin
- Subjects
biology ,business.industry ,Hepacivirus ,Environmental health ,Nucleic acid ,Medicine ,Hematology ,General Medicine ,Computational biology ,business ,biology.organism_classification ,Donor screening - Published
- 2005
30. Assessing the accuracy of three viral risk models in predicting the outcome of implementing HIV and HCV NAT donor screening in Australia and the implications for future HBV NAT
- Author
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Anthony J. Keller, T. J. Cobain, Philip Kiely, Clive R. Seed, Anthea Cheng, Bolton Wv, and S. Ismay
- Subjects
Adult ,Oncology ,Hepatitis B virus ,medicine.medical_specialty ,HBsAg ,Immunology ,Population ,Blood Donors ,HIV Infections ,Hepacivirus ,medicine.disease_cause ,Risk Assessment ,Internal medicine ,HIV Seropositivity ,Prevalence ,medicine ,Humans ,Mass Screening ,Immunology and Allergy ,Viremia ,education ,Screening procedures ,Probability ,Retrospective Studies ,Immunoassay ,education.field_of_study ,biology ,business.industry ,Australia ,HIV ,Transfusion Reaction ,virus diseases ,Hematology ,Models, Theoretical ,Hepatitis B ,biology.organism_classification ,Hepatitis C ,Residual risk ,Outcome and Process Assessment, Health Care ,Nat ,Lentivirus ,RNA, Viral ,Viral disease ,business - Abstract
BACKGROUND : Risk modeling is now the most practical method of estimating the residual risk of viral transmission in developed countries. One method of assessing the accuracy of a risk model is to measure the observed against the predicted outcome after implementing a new screening method. The primary objective of this paper is to assess the accuracy of three published models in predicting the impact of implementing HIV and HCV NAT in Australia. STUDY DESIGN AND METHODS : Viral screening data on Australian donors for 2000 and 2001 were retrospectively analyzed. The data were applied to the three models to estimate the risk of transmission and predicted NAT yield for HIV, HCV, and HBV. RESULTS : The median risk estimates for the three models were 1 in 3,415,000 for HIV NAT, 1 in 911,000 for HCV NAT, and 1 in 483,000 for HBsAg. The predicted NAT yield for the three models ranged from 0.17 to 0.30 per million donations for HIV, 1.20 to 5.55 for HCV, and 0.47 to 1.01 for HBV. The observed NAT yield was not significantly different from the expected yield with any of the three models for either HIV or HCV. CONCLUSIONS : First, the residual risk in Australian donors is small in comparison with other transfusion complications and comparable to or lower than the risk in US and European nonremunerated donors. Second, mathematical risk modeling has sufficient precision to be used as a predictive tool for risk-benefit assessments of novel screening procedures. Finally, in relation to the case for implementing HBV NAT and/or anti-HBc in Australia, we conclude that at present, there is inadequate information about our donor population to perform an evidence-based risk-benefit analysis.
- Published
- 2002
31. First reported case of transfusion-transmitted Ross River virus infection
- Author
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Joanne Pink, Anthony J. Keller, Gary K Dowse, Veronica C. Hoad, David J. Speers, Clive R. Seed, Michael D. A. Lindsay, and Helen M. Faddy
- Subjects
Ross river virus infection ,business.industry ,Alphavirus Infections ,Myelodysplastic Syndromes ,Australia ,Ross River virus ,Medicine ,Humans ,Blood Donors ,General Medicine ,business ,Erythrocyte Transfusion ,Virology - Published
- 2014
32. Infectivity of blood components from donors with occult hepatitis B infection - results from an Australian lookback programme
- Author
-
Clive R. Seed, Barbara Bell, Joanne Pink, Philip Kiely, Anthony J. Keller, and R. Maloney
- Subjects
medicine.medical_specialty ,Hepatitis B virus ,Blood transfusion ,medicine.medical_treatment ,Blood Donors ,medicine.disease_cause ,Antigen ,Internal medicine ,medicine ,Prevalence ,Humans ,Hepatitis B Antibodies ,Infectivity ,Hepatitis B Surface Antigens ,biology ,Transmission (medicine) ,business.industry ,Australia ,Transfusion Reaction ,Hematology ,General Medicine ,Hepatitis B ,Middle Aged ,medicine.disease ,Virology ,Cryoprecipitate ,DNA, Viral ,biology.protein ,Antibody ,business - Abstract
Background and objectives Previous studies have demonstrated that transfusedblood components from donors with occult hepatitis B virus infection (OBI) arepotentially infectious. This study reports the results of an Australian lookback pro-gramme for the period subsequent to the commencement of individual donationHBV NAT in July 2010 and estimates the HBV transmission rate for componentsfrom two categories of donors, confirmed OBI and HBV inconclusive (anti-HBcreactive with non-discriminated NAT result).Materials and methods Using the results of lookback investigations, weestimated HBV transmission rates by donor category and type of componenttransfused based on the prevalence of antibodies to HBV core antigen (anti-HBc)in recipients adjusted for the estimated prevalence in the general population.Results After subtracting the background anti-HBc rate, we derived an adjustedtransmission rate (all components) with lower and upper bounds as follows:0 85% (0 00–2 35%) for OBI donors, 2 83% (1 23–4 33%) for inconclusive donorsand 1 81% (0 21–3 31%) for total (OBI and inconclusive) donors. The medianadjusted transmission rate for total donors was higher (but not statistically) forplasma (3 01%) than RCCs (2 86%), but there was no evidence of transmissionfor cryoprecipitate or platelets (0% for both components).Conclusion Our lookback study suggests a low (0 2–3 3%) but measurable rateof HBV transmission in Australia associated with donors with OBI and sup-ports published evidence that at least some blood component types from OBIdonors, including a proportion undetectable by ID-NAT can transmit HBV bytransfusion.Keywords: Hepatitis B virus, Lookback, NAT testing, transfusion transmission.
- Published
- 2014
33. Should DEHP be eliminated in blood bags?
- Author
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Michael J. Germain, Jørgen Georgsen, S. Biagini, S. Ismay, Patricia Scuracchio, D. Dernis, Veerle Compernolle, S. Bégué, Janet Yuen Ha Wong, Simon Panzer, Ralph R. Vassallo, S. Villa, D. de Korte, S. Wendel, Paolo Rebulla, H. Gulliksson, Joanne Pink, Joan Cid, Rebecca Cardigan, Anthony J. Keller, C. Buchta, Naomi L.C. Luban, Mark K. Fung, C. Tooke, Miquel Lozano, Louis Thibault, Richard J. Benjamin, P. F. van der Meer, H. W. Reesink, Minoko Takanashi, and E. Raspollini
- Subjects
Male ,endocrine system ,medicine.medical_specialty ,Adolescent ,Metabolite ,Population ,Urine ,Pharmacology ,chemistry.chemical_compound ,Plasticizers ,Internal medicine ,Diethylhexyl Phthalate ,medicine ,Humans ,education ,Whole blood ,education.field_of_study ,Blood Specimen Collection ,Anogenital distance ,Phthalate ,Hematology ,General Medicine ,Haemolysis ,Endocrinology ,chemistry ,Toxicity ,Female - Abstract
Whole blood for transfusion was initially collected in glass bottles, but these are fragile, heavy to transport and prone to bacterial contamination. After the Second World War, Carl Walter experimented with plastics for collection and storage of blood, and found that di-ethylhexyl phthalate (DEHP) as plasticizer for polyvinyl chloride (PVC) had the most favourable properties [1, 2]. The breakage rate of frozen plasma in DEHP PVC is low, and the better storage properties of red cell concentrates in DEHP PVC are explained by leaching of plasticizer into the lipid bilayer of these cells, thereby improving membrane stability resulting in lower haemolysis rates [3]. However, ever since the 1970s, scientific and public concern has been raised on the toxicity of DEHP for transfused patients [4, 5]. DEHP and its metabolite mono ethylhexyl phthalate (MEHP) are associated with impairment of reproduction in animal models, resulting in testicular dysgenesis syndrome in (male) rodents, as reviewed in depth elsewhere [6], and particularly, neonates are susceptible to the risks of high exposure to DEHP. Due to the omnipresence of phthalates (not only DEHP), and some important differences in DEHP metabolism between animal models and humans [6], it is difficult to estimate the effect of DEHP toxicity in humans. A study of 234 young men showed no effect of MEHP in urine on their reproductive function [7], but others showed that DNA damage in sperm was associated with the MEHP concentration (after adjustment for other DEHP oxidative metabolites) [8]. In vitro human testis explants incubated with DEHP or MEHP showed that both significantly inhibited testosterone production [9], and a study of 881 men showed a 9% lower free androgen index between the lowest and the highest quartile of the proportion DEHP excreted as MEHP (P = 0.02) [10]. Also, an association was found between urinary MEHP concentrations and lower free T4 and lower total T3 thyroid hormones in adult men [11]. In addition to these effects on hormonal level in adult males, DEHP and its metabolites can have an effect on fetal development, with a shorter anogenital distance associated with higher metabolite concentrations in urine samples collected during pregnancy [12]. Others found no difference in T4 and testosterone levels, as well as no effect on phallic length in 13 male adolescents 14– 16 years of age that had underwent ECMO treatment in their neonatal period, as compared to ageand sexmatched controls [13]. Also, DEHP/MEHP exposure (as detected in cord blood) was significantly associated with a shorter pregnancy duration [14]. Despite these associations, they should be interpreted with care. DEHP is not the only phthalate that may cause these effects; studies that showed no association may not have been published; DEHP administered through the intestinal tract is converted to MEHP in a higher degree as by intravenously [15]; and because phthalates are everywhere in our environment, comparisons are less versus more, rather than absent versus present. DEHP-plasticized PVC has long been used as standard material for a wide range of applications, like packing foil (including food), building material, toys and plastic devices. DEHP is considered to be a ubiquitous environmental contaminant and is present in air, dust, water, soil and food. The latter is considered to be the main source of DEHP intake for the general population. The current daily intake is estimated to be between 2 and 5 lg/kg for adults and between 4 to 8 lg/kg for children [16, 17]. Various measures to reduce the use of DEHP-plasticized PVC have indeed resulted in a 2-times lower exposure to DEHP in the general population over the last two decades [18]. DEHP is applied in many medical devices, and depending on the procedure, patients can be exposed to high (peak) concentrations of DEHP, in the order of 1 mg per kg of bodyweight. One of the sources of DEHP exposure is the leaching from the blood bags into the content of the container, particularly into lipophilic solutions such as plasma [19], but also red cells. Therefore some patient groups, including pregnant or nursing women and children, are considered to be most at risk of possible harmful effects attributable to DEHP exposure by medical treatments. DEHP is broken down into various metabolites. First, it is hydrolysed to MEHP and then further oxidized to mono-(2-ethyl-5-hydroxyhexyl) phthalate (5OH-MEHP)
- Published
- 2014
34. Variant Creutzfeldt-Jakob disease in Australian blood donors: estimation of risk and the impact of deferral strategies
- Author
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Elizabeth M. Dax, Anthony J. Keller, Patty Correll, Anthony Gust, Clive R. Seed, John M. Kaldor, Matthew Law, and Buring Ml
- Subjects
Hepatitis B virus ,business.industry ,Transmission (medicine) ,Hematology ,General Medicine ,Window period ,Hepatitis C ,Hepatitis B ,medicine.disease_cause ,medicine.disease ,Immunology ,Medicine ,Viral disease ,Risk factor ,business ,Deferral ,Demography - Abstract
Background and Objectives In Australia, a policy of deferring donors who have lived in the UK for longer than 6 months between 1980 and 1996 has been instituted to reduce the theoretical risk of transmitting variant Creutzfeldt-Jakob disease (vCJD) through the blood supply. The objective of this report was to refine estimates of the possible risks and benefits of donor-deferral strategies that are aimed at avoiding transmission of vCJD. Materials and Methods Estimates of the effect of donor deferral on the blood supply in Australia were based on a 1998 survey of blood donors. The number of donations from donors potentially infected with vCJD and excluded by donor deferral was estimated based on published estimates of the size of the vCJD epidemic in the UK and assuming that the risk of vCJD in Australian blood donors was proportional to the time lived in the UK between 1980 and 1996. The possible increased number of blood donations that were infected with human immunodeficiency virus (HIV), hepatitis C virus (HCV) or hepatitis B virus (HBV) and made during a window period (as a result of increased donations from first-time donors) was estimated using published methods. Results A strategy of deferring donations in Australia from people who have lived in the UK for 6 months or longer, between 1980 and 1996, was estimated to result in exclusion of 5·3% of all blood donations, corresponding to 50 100 donations in 1998. It was estimated that the annual number of blood donations made by donors potentially infected with vCJD is 1·15 (range 0·02–31·1, based on the uncertainty in the UK prevalence estimate). Donor deferral was estimated to remove 0·92 (range 0·02–25·1) of these donations. Replacement of 33%, 50% and 100% of excluded donations by donations from first-time donors, was estimated to result in an increase of 0·0010, 0·0019 and 0·0044, respectively, of HIV-infected donations per year donated during the window period; in an increase of 0·021, 0·038 and 0·089, respectively, of HCV-infected donations per year; and in an increase of 0·18, 0·33 and 0·76, respectively, of HBV-infected donations per year. Conclusions The large uncertainties involved in these analyses mean that estimates must be interpreted cautiously, but the data does suggest that donor deferral may exclude more donations from donors potentially infected with vCJD than the corresponding increase, caused by donor replacement, of window-period donations possibly infected with HIV, HCV or HBV.
- Published
- 2001
35. A refined method for estimating the size of the potential blood donor pool in Australia
- Author
-
Tarana T A, Lucky, Anthony J, Keller, Clive R, Seed, June, Lee, Claire, Styles, Joanne, Pink, and David P, Wilson
- Subjects
Adult ,Aged, 80 and over ,Male ,Young Adult ,Adolescent ,Australia ,Humans ,Blood Donors ,Female ,Middle Aged ,Aged ,Donor Selection - Abstract
To be eligible to donate blood, potential donors must meet certain eligibility criteria to ensure safety to the donor and to the blood supply. In Australia, there is no reliable estimate of the size of the donor-eligible population. This study uses a refinement to a published method to determine the population prevalence of donor-exclusion factors and subsequently estimates the size of the potential donor pool in Australia.A total of 70 donor-exclusion factors (in addition to age) were identified. The donor-eligible population was estimated by subtracting the prevalence of the exclusion factors from the total population. Prevalence of the donor-exclusion factors was adjusted for age, deferral period, and overlap of multiple conditions. Overlap was adjusted by extending a published random-probability model according to known association of epidemiologic data on overlapping conditions.The most prevalent (deferral period-adjusted) donor-exclusion factor among the 16- to 80-year-old Australian population was variant Creutzfeldt-Jakob disease-related travel risk (6.8%) followed by upper respiratory tract infections (6.4%). After exclusion of all factors, and accounting for overlapping factors, 62% of 16- to 80-year-olds or 47.3% of the total population were donor eligible in Australia.We developed a refined method for estimating the size of the donor-eligible population. Applying this method to Australia, we estimate that approximately 10.7 million people (62% of the 16- to 80-year-olds) were eligible to donate blood in Australia in 2012.
- Published
- 2013
36. Understanding noncompliance with selective donor deferral criteria for high-risk behaviors in Australian blood donors
- Author
-
Tarana T A, Lucky, Clive R, Seed, Daniel, Waller, June F, Lee, Ann, McDonald, Handan, Wand, Stephen, Wroth, Glen, Shuttleworth, Anthony J, Keller, Joanne, Pink, and David P, Wilson
- Subjects
Adult ,Male ,Sex Workers ,Australia ,Blood Donors ,HIV Infections ,Middle Aged ,Truth Disclosure ,Donor Selection ,Drug Users ,Young Adult ,Risk-Taking ,Risk Factors ,Surveys and Questionnaires ,Humans ,Female ,Guideline Adherence ,Homosexuality, Male ,Aged - Abstract
Using a predonation screening questionnaire, potential blood donors are screened for medical or behavioral factors associated with an increased risk for transfusion-transmissible infection. After disclosure of these risks, potential donors are deferred from donating. Understanding the degree of failure to disclose full and truthful information (termed noncompliance) is important to determine and minimize residual risk. This study estimates the prevalence of, and likely reasons for, noncompliance among Australian donors with the deferrals for injecting drug use, sex with an injecting drug user, male-to-male sex, sex worker activity or contact, and sex with a partner from a high-HIV-prevalence country.An anonymous, online survey of a nationally representative sample of Australian blood donors was conducted. Prevalence of noncompliance with deferrable risk categories was estimated. Factors associated with noncompliance were determined using unadjusted and adjusted odds ratios.Of 98,044 invited donors, 30,790 donors completed the survey. The estimated prevalence of overall noncompliance (i.e., to at least one screening question) was 1.65% (95% confidence interval CI, 1.51%-1.8%). Noncompliance with individual deferrals ranged from 0.05% (sex work) to 0.54% (sex with an injecting drug user). The prevalences of the disclosed exclusionary risk behaviors were three to 14 times lower than their estimated prevalence in the general population.The prevalence of noncompliance is relatively low but our estimate is likely to be a lower bound. The selected high-risk behaviors were substantially less common in blood donors compared to the general population suggesting that self-deferral is effective. Nevertheless, a focus on further minimization should improve the blood safety.
- Published
- 2013
37. Compliance with the current 12-month deferral for male-to-male sex in Australia
- Author
-
Clive R. Seed, Tarana T.A. Lucky, Handan Wand, June Lee, Ann McDonald, Daniel Waller, David Wilson, Joanne Pink, Anthony J. Keller, and S. Wroth
- Subjects
Adult ,Male ,Risk ,medicine.medical_specialty ,Time Factors ,Adolescent ,Blood Donors ,HIV Infections ,Compliance (psychology) ,Young Adult ,Multiple sexual partners ,Surveys and Questionnaires ,Epidemiology ,medicine ,Humans ,Homosexuality, Male ,Deferral ,Sexual Abstinence ,Motivation ,business.industry ,Australia ,Transfusion Reaction ,Hematology ,General Medicine ,Middle Aged ,Hepatitis B ,Surgery ,Donation ,Female ,Guideline Adherence ,business ,Sexual contact ,Demography - Abstract
Background and Objectives In Australia since 2000, donors are deferred for12 months since last male-to-male sexual contact. There is no estimate of theprevalence of non-compliance (i.e. failure to disclose a risk during the predona-tion interview which would lead to deferral) with the policy in Australia; how-ever, published studies elsewhere indicate a range of 0 8–11%. We investigatedthe rate of, timing and motivation for non-compliance.Materials and Methods A nationally representative sample of donors who hadmade a recent donation negative for transfusion-transmissible infection testingwas surveyed using an anonymous, online instrument. Non-compliance was con-sidered as a ‘yes’ response to the current screening question. Non-compliers wererequested to define the timing of the last sexual contact relevant to their mostrecent donation. Univariate and multivariate regression analyses were used todefine factors associated with non-compliance.Results Of 14 476 responses from male donors, 34 (0 23%, 95% CI: 0 16–0 33%)were non-compliant of whom 24 (0 17%, 95% CI: 0 11–0 25%) had contactwithin 6 months of donation. Factors significantly associated with non-compli-ance included: multiple sexual partners, history of injecting drug use, perceptionof a lack of privacy during interview and preference for a computer-based ques-tionnaire.Conclusion Our study confirms high compliance (>99 7%) to the 12-month defer-ral for male-to-male sex in Australia providing reassuring evidence for the effi-cacy of the screening question. Issues of ‘privacy’ and ‘discomfort’ associated withdisclosure suggest the use of validated audio computer-assisted structured inter-view as a possible option for improving compliance with the donor questionnaire.Key words: donors, epidemiology, transfusion-transmissible infection.
- Published
- 2013
38. An 8-week course of 45 mg of carbonyl iron daily reduces iron deficiency in female whole blood donors aged 18 to 45 years: results of a prospective randomized controlled trial
- Author
-
Denese C, Marks, Joanna, Speedy, Kathryn L, Robinson, Tania, Brama, Hugh R, Capper, Phillip, Mondy, and Anthony J, Keller
- Subjects
Adult ,Young Adult ,Adolescent ,Anemia, Iron-Deficiency ,Iron ,Double Bind Interaction ,Ferritins ,Humans ,Female ,Prospective Studies ,Middle Aged ,Drug Administration Schedule ,Iron Compounds - Abstract
Blood donation is known to contribute to iron deficiency in regular blood donors. This study investigated the safety and efficacy of postdonation iron replacement to mitigate iron deficiency in blood donors.A total of 282 female whole blood donors aged 18 to 45 were prospectively randomized in a double-blinded placebo controlled trial to receive an 8-week postdonation course of carbonyl iron (45 mg daily) or placebo. The primary endpoint was prevalence of iron deficiency (ferritin15 ng/mL) at 12 weeks postdonation. Secondary endpoints were eligibility to donate based on capillary hemoglobin (Hb) and incidence of gastrointestinal (GI) complaints.Ferritin levels at Week 12 were significantly higher in donors receiving carbonyl iron (17.0 ± 10.9 ng/mL) compared with those receiving placebo (10.6 ± 8.4 ng/mL; p 0.001). The proportion of iron-deficient donors was significantly lower in the carbonyl iron group (51.9%) compared to the placebo (80.5%; p 0.001). The mean Hb level in the carbonyl iron group (134.6 ± 8.7 g/L) was significantly higher than in the placebo arm (130.0 ± 9.9 g/L; p 0.001), significantly improving eligibility to donate at Week 12. Significantly more donors receiving carbonyl iron had at least one GI side effect (p 0.001). Importantly, 86.7% of donors receiving carbonyl iron indicated that they would take iron on an ongoing basis.An 8-week postdonation course of 45 mg of carbonyl iron significantly reduced iron deficiency and was well tolerated in female whole blood donors. Postdonation iron replacement may have a role in a broader strategy to optimize donor iron status.
- Published
- 2013
39. Post‐transfusion hepatitis revisited
- Author
-
Sally Thomas, Anthony J. Keller, Kenneth G Kenrick, Susan L Ismay, Gordon T Archer, Yvonne E. Cossart, Annette Fellows, and Brenton R Wylie
- Subjects
medicine.medical_specialty ,Hepatitis C virus ,Blood Donors ,Hepacivirus ,medicine.disease_cause ,Internal medicine ,medicine ,Humans ,Hepatitis Antibodies ,Prospective Studies ,Cardiac Surgical Procedures ,Risk factor ,Retrospective Studies ,Hepatitis ,biology ,business.industry ,Transfusion Reaction ,Alanine Transaminase ,Western Australia ,General Medicine ,Hepatitis C ,Hepatitis C Antibodies ,Hepatitis B ,medicine.disease ,Immunology ,biology.protein ,Viral disease ,New South Wales ,Antibody ,business ,Viral hepatitis - Abstract
OBJECTIVE To evaluate the risk of post-transfusion and postoperative non-A non-B hepatitis in Australia immediately before the introduction of screening for hepatitis C. DESIGN Retrospective testing of blood samples from a prospective study of cardiac surgery patients. Samples were taken from transfusion recipients and non-transfused controls at regular intervals for 12 months after surgery during 1987-1989. For all donor, recipient and control samples, alanine aminotransferase (ALT) levels were measured and tests for antibody to hepatitis B (anti-HBc, anti-HBs) and, when available, to hepatitis C (anti-HCV) were performed. SETTING Cardiac surgery units. PARTICIPANTS Participants were included if they lived in the metropolitan area, and had not had a transfusion in the past year. MAIN OUTCOME MEASURES Post-transfusion hepatitis (two consecutive samples showing raised ALT levels, > 90 IU/L with no other known cause); hepatitis C infection and carriage (antibody to hepatitis C). RESULTS Post-transfusion hepatitis occurred in 1.1% of 736 recipients of blood not screened for hepatitis C (i.e., two cases per 1000 unscreened units given). No hepatitis occurred in 514 controls. Seven of the eight patients with post-transfusion hepatitis seroconverted to hepatitis C virus infection. Seven of the 26 anti-HCV-positive donations transmitted hepatitis C, six of these were positive by recombinant immunoblot assay (RIBA) (one by second generation testing only) and one was RIBA indeterminate. Nineteen were RIBA non-reactive; one transmitted hepatitis but the recipient did not develop anti-HCV, although hepatitis C RNA was detected in the donation. Serum ALT was raised in four of the six infective donations. CONCLUSIONS Hepatitis C virus infection accounted for almost all cases of non-A non-B post-transfusion hepatitis. First generation anti-HCV tests detected about 85% of infective donations. Surrogate testing of donations by ALT or anti-HBc offers no additional advantage.
- Published
- 1995
40. Geographical distribution of hepatitis C virus genotypes in blood donors: an international collaborative survey
- Author
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F. McOmish, Anthony J. Keller, Clive R. Seed, E. A. C. Follett, P L Yap, T Krusius, R Naukkarinen, T. J. Cobain, E. Kolho, and B C Dow
- Subjects
Microbiology (medical) ,Genotype ,International Cooperation ,Hepatitis C virus ,Molecular Sequence Data ,Blood Donors ,Hepacivirus ,medicine.disease_cause ,Virus ,Serology ,Flaviviridae ,medicine ,Humans ,Base Sequence ,biology ,Asia, Eastern ,Genetic Variation ,Transfusion Reaction ,Hepatitis C ,biology.organism_classification ,medicine.disease ,Virology ,Europe ,Eastern european ,Immunology ,RNA, Viral ,Egypt ,Restriction fragment length polymorphism ,Polymorphism, Restriction Fragment Length ,Research Article - Abstract
The frequency of infection with the six classified major genotypes of hepatitis C virus (HCV) was investigated in 447 infected volunteer blood donors from the following nine countries: Scotland, Finland, The Netherlands, Hungary, Australia, Egypt, Japan, Hong Kong, and Taiwan. Viral sequences in plasma from blood donors infected with HCV were amplified in the 5'-noncoding region and were typed by restriction fragment length polymorphism analysis. Electrophoresis of DNA fragments produced by cleavage with HaeIII-RsaI and ScrFI-HinfI allowed HCV types 1 (or 5), 2, 3, 4, and 6 to be identified. Further analysis with MvaI-HinfI allowed sequences of the type 5 genotype to be distinguished from sequences of the type 1 genotype. Types 1, 2, and 3 accounted for almost all infections in donors from Scotland, Finland, The Netherlands, and Australia. Types 2 and 3 were not found in the eastern European country (Hungary), where all but one of the donors were infected with type 1. Donors from Japan and Taiwan were infected only with type 1 or 2, while types 1, 2, and 6 were found in those from Hong Kong. HCV infection among Egyptians was almost always by type 4. Donors infected with HCV type 1 showed broad serological reactivity with all four antigens of the second generation Chiron RIBA-2 assay (Chiron Corporation, Emeryville, Calif.), while infection with divergent HCV genotypes elicited antibodies mainly reactive to c22-3 and c33c. Reactivities with antibodies 5-1-1 and c100-3 were infrequent and were generally weak, irrespective of the geographical origin of the donor. Because the envelope region of HCV is even more variable than the NS-4 region, it is likely that vaccines based on these proteins need to be multivalent and perhaps specifically adapted for different geographical regions.
- Published
- 1994
41. Hemoglobin and iron indices in nonanemic premenopausal blood donors predict future deferral from whole blood donation
- Author
-
Sant-Rayn, Pasricha, Zoe K, McQuilten, Anthony J, Keller, and Erica M, Wood
- Subjects
Time Factors ,Anemia, Iron-Deficiency ,Databases, Factual ,Iron ,Blood Donors ,Middle Aged ,Hemoglobins ,Hepcidins ,Premenopause ,Receptors, Transferrin ,Humans ,Female ,Prospective Studies ,Antimicrobial Cationic Peptides ,Follow-Up Studies - Abstract
Iron deficiency anemia is an important reason for blood donor deferral. We prospectively determined whether screening donors with hemoglobin (Hb) and iron indices before donation can predict subsequent deferral due to anemia.We recruited premenopausal, eligible (nonanemic) female donors. Hb, ferritin, soluble transferrin receptor (sTfR), and hepcidin were measured, and the sTfR/(log)ferritin (sTfR-F) index was calculated. After 6 months, the donor database was reviewed and whether donors had returned and undergone successful donation was recorded.Of donors, 59 of 261(22.6%) were iron depleted (ferritin15 ng/mL). Iron-depleted donors had donated more often in the previous year, were younger, and had lower Hb. After a minimum of 6 months, 145 eligible donors had returned; of these 10 (6.9%) were deferred for anemia. Donors who developed anemia had significantly lower Hb, ferritin, and hepcidin and higher sTfR and sTfR-F at baseline. The area under the receiver operating characteristic curve for Hb as a predictor of deferral was 0.86, and for ferritin was 0.88. Hb of less than 130 g/L and ferritin of less than 10 ng/mL combined had sensitivity 80% and specificity 96% in predicting deferral.Screening with Hb and iron indices enables prediction of donors at risk of subsequent anemia and who would most benefit from prevention strategies.
- Published
- 2011
42. No evidence of a significantly increased risk of transfusion-transmitted human immunodeficiency virus infection in Australia subsequent to implementing a 12-month deferral for men who have had sex with men
- Author
-
Clive R, Seed, Philip, Kiely, Mathew, Law, and Anthony J, Keller
- Subjects
Male ,Time Factors ,Australia ,Health Plan Implementation ,Transfusion Reaction ,Blood Donors ,HIV Infections ,Donor Selection ,Sex Factors ,Risk Factors ,HIV-1 ,Prevalence ,Humans ,Blood Transfusion ,Preventive Medicine ,Homosexuality, Male ,Program Evaluation ,Retrospective Studies - Abstract
Male-to-male sex is the predominant route of human immunodeficiency virus (HIV) transmission in Australia and since the early 1980s blood services in Australia have deferred donors for this practice for at least 5 years. This retrospective analysis assesses the impact on HIV prevalence of implementing an abridged 12-month deferral for male-to-male sex.The prevalence of HIV among blood donors for 5-year periods before (Period 1) and after (Period 2) implementing the revised 12-month deferral was compared. Using deidentified data from postdonation interviews with HIV-positive donors the proportion disclosing male-to-male sex as a risk factor was compared for the two periods.Twenty-four HIV-positive donations were identified among 4,025,571 donations in Period 1 compared with 24 among 4,964,628 donations in Period 2 (p=0.468). The proportion of HIV-positive donors with male-to-male sex as a risk factor in Period 1 was 2 in 15 (13.3%), which was not significantly different from the proportion in Period 2, 5 in 16 (31.25%; p=0.22). All five men who have sex with men risk HIV infections during Period 2 were from donors whose risk was within the 12-month criterion for acceptability, who would have been deferred had they provided a complete history.We found no evidence that the implementation of the 12-month deferral for male-to-male sex resulted in an increased recipient risk for HIV in Australia. The risk of noncompliance to the revised deferral rather than its duration appears to be the most important modifier of overall risk.
- Published
- 2010
43. Can blood tranfusion transmit cancer? A literature review
- Author
-
June Lee, Clive R. Seed, Anthony J. Keller, and Hung Yang
- Subjects
medicine.medical_specialty ,Blood transfusion ,business.industry ,medicine.medical_treatment ,Biochemistry (medical) ,Clinical Biochemistry ,MEDLINE ,Cancer ,Transfusion Reaction ,Blood Donors ,Hematology ,Malignancy ,medicine.disease ,Malignant disease ,Surgery ,Transfusion reaction ,Neoplasms ,medicine ,Humans ,Cancer risk ,Intensive care medicine ,business ,Precancerous Conditions ,Allogeneic transfusion - Abstract
Blood services around the world face increasing challenges in recruiting voluntary blood donors. With increasing donor restrictions and ageing populations, it is essential to look for any existing restrictions that may be relaxed in the light of currently available evidence. We propose that one such restriction is the exclusion of blood donors with a history of a malignancy. Most blood services apart from the United States and Australia continue the historical precaution of permanently excluding donors with a history of cancer, despite the absence of any convincing reports of cancer transmission among the millions of allogeneic blood transfusions performed since the advent of blood banking. In 2007, workers in Scandinavia published convincing data from the SCANDAT (Scandinavian Donations and Transfusions) database that showed no increase in cancer risk among recipients of blood from "precancerous" donors (ie, donors who were later diagnosed with cancer within 5 years of donating) vs recipients of blood from other donors. This review aims to reconcile this finding with other data available in the published literature that is pertinent to the risk of transmitting cancer via blood transfusion, with a view to establishing that there is now sufficient evidence to support the acceptance of carefully selected blood donors with a history of malignant disease.
- Published
- 2010
44. No evidence of a significantly increased risk of transfusion-transmitted human immunodeficiency virus infection in Australia subsequent to implementing a 12-month deferral for men who have had sex with men (CME): ASSESSING THE IMPACT OF 12-MONTH DEFERRAL FOR MSM
- Author
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Clive R. Seed, Mathew Law, Anthony J. Keller, and Philip Kiely
- Subjects
medicine.medical_specialty ,Pediatrics ,biology ,Donor selection ,business.industry ,Immunology ,Hematology ,medicine.disease ,biology.organism_classification ,Acquired immunodeficiency syndrome (AIDS) ,Donation ,Epidemiology ,medicine ,Immunology and Allergy ,Viral disease ,Risk factor ,business ,Deferral ,Sida - Abstract
BACKGROUND: Male-to-male sex is the predominant route of human immunodeficiency virus (HIV) transmission in Australia and since the early 1980s blood services in Australia have deferred donors for this practice for at least 5 years. This retrospective analysis assesses the impact on HIV prevalence of implementing an abridged 12-month deferral for male-to-male sex. STUDY DESIGN AND METHODS: The prevalence of HIV among blood donors for 5-year periods before (Period 1) and after (Period 2) implementing the revised 12-month deferral was compared. Using deidentified data from postdonation interviews with HIV-positive donors the proportion disclosing male-to-male sex as a risk factor was compared for the two periods. RESULTS: Twenty-four HIV-positive donations were identified among 4,025,571 donations in Period 1 compared with 24 among 4,964,628 donations in Period 2 (p = 0.468). The proportion of HIV-positive donors with male-to-male sex as a risk factor in Period 1 was 2 in 15 (13.3%), which was not significantly different from the proportion in Period 2, 5 in 16 (31.25%; p = 0.22). All five men who have sex with men risk HIV infections during Period 2 were from donors whose risk was within the 12-month criterion for acceptability, who would have been deferred had they provided a complete history. CONCLUSIONS: We found no evidence that the implementation of the 12-month deferral for male-to-male sex resulted in an increased recipient risk for HIV in Australia. The risk of noncompliance to the revised deferral rather than its duration appears to be the most important modifier of overall risk.
- Published
- 2010
45. Relapsing vivax malaria despite chemoprophylaxis in two blood donors who had travelled to Papua New Guinea
- Author
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Anne M Pickworth, Anthony J. Keller, Robert Harley, Jacqueline T Coughlin, and Clive R. Seed
- Subjects
Adult ,Male ,Pediatrics ,medicine.medical_specialty ,Blood transfusion ,medicine.medical_treatment ,Plasmodium vivax ,Antibodies, Protozoan ,Blood Donors ,Enzyme-Linked Immunosorbent Assay ,Chemoprevention ,Antimalarials ,Papua New Guinea ,Pharmacotherapy ,parasitic diseases ,medicine ,Malaria, Vivax ,Humans ,Blood Transfusion ,Travel ,biology ,business.industry ,Australia ,New guinea ,General Medicine ,Middle Aged ,medicine.disease ,biology.organism_classification ,Donation ,Chemoprophylaxis ,Immunology ,Vivax malaria ,business ,Malaria - Abstract
Two Australian blood donors were diagnosed with relapsing Plasmodium vivax malaria 5 and 15 months, respectively, after their most recent travel to a malaria-endemic country. Common features included travel to Papua New Guinea (specifically, the Kokoda Trail); full compliance with recommended malaria chemoprophylaxis; and negative results on malaria antibody testing at the time of donation. Although all fresh blood components from the two donors issued on the basis of these negative results were recalled before transfusion, these cases underscore the increased potential for relapse of P. vivax in donors returning from malaria-endemic countries, as well as the inability to identify the potential for relapse using current malarial screening tests.
- Published
- 2009
46. Prediction of hepatitis C virus infectivity in seropositive Australian blood donors by supplemental immunoassays and detection of viral RNA
- Author
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Gregory J. Cooper, Anthony J. Keller, David S. Vallari, Jean-Pierre Allain, Mary C. Kuhns, Patrick J. Coghlan, Stephen R. Delaney, Kenneth Whitson., and Kenneth G Kenrick
- Subjects
Infectivity ,medicine.diagnostic_test ,Hepatitis C virus ,Immunology ,virus diseases ,Cell Biology ,Hematology ,Biology ,medicine.disease_cause ,Biochemistry ,Virology ,digestive system diseases ,Virus ,law.invention ,Antigen ,law ,Immunoassay ,medicine ,Supplemental Testing ,Viral disease ,Polymerase chain reaction - Abstract
The prevalence of anti-hepatitis C virus (HCV) enzyme immunoassay (EIA)- positive in 167,511 Australian volunteer blood donors from Adelaide, Melbourne, Perth, and Sydney was 0.78%. One thousand two-hundred and eighteen EIA-positive serum samples were assessed by supplemental tests including a blocking EIA and two peptide EIAs corresponding to major epitopes of the HCV C-100–3 antigen. Seven hundred and eighteen samples (59%) were negative by supplemental testing; no evidence of reactivity with other HCV gene products or HCV RNA detected by cDNA polymerase chain reaction was found in selected samples from this group. In contrast, of 43 samples randomly selected from 400 samples (32.8%) positive by supplemental testing, 88% were reactive with HCV 33-C or core antigens and 52% contained HCV RNA, suggesting contact with HCV and infectivity of most donors in this group. Most samples equivocal by supplemental testing reacted only with C-100 and not with other HCV antigens when tested by dot immunoblot assay. Only 21% had detectable HCV RNA. The battery of assays used in this study indicated that approximately 32% of HCV EIA repeatably reactive serum samples were serologically related to HCV, corresponding to a 0.25% prevalence of potentially infectious donors.
- Published
- 1991
47. Biobanks of blood from donors and recipients of blood products
- Author
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Silvia Giovanelli, I. Thomas, Christopher Prowse, Paolo Rebulla, L. Amorim, Steve Kleinman, Celso Bianco, R. Norda, B. C. Dow, Jean-Claude Osselaer, Jørgen Georgsen, M. Letowska, Anthony J. Keller, Susan L. Stramer, Lucilla Lecchi, F. Mozzi, Wolfgang R. Mayr, G. Andreu, Lisa Jarvis, Roger Y. Dodd, F. Davidson, Patrick Coghlan, Jaap-Jan Zwaginga, B. Tait, J. A. van Hilten, S. Wendel, Olof Åkerblom, M. Beaten, Michael P. Busch, Peter Flanagan, G. Henn, Micheline Lambermont, T. Mäki, Barbara Butti, Ewa Brojer, O. Flesland, Jean-Jacques Lefrère, Catherine A. Hyland, Tom Krusius, C. P. Engelfriet, H. W. Reesink, M. Wsolak, Pascal Morel, Y. Qiu, Gastroenterology and Hepatology, Other departments, and Landsteiner Laboratory
- Subjects
medicine.medical_specialty ,Internationality ,business.industry ,Blood Donors ,Hematology ,General Medicine ,Biobank ,Blood Preservation ,medicine ,Blood Banks ,Humans ,Safety ,Intensive care medicine ,business - Abstract
Udgivelsesdato: 2008-Apr
- Published
- 2008
48. Reducing the risk of transfusion-transmissible viral infection through blood donor selection: the Australian experience 2000 through 2006
- Author
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Helen Ingham, Erica M. Wood, Mark N. Polizzotto, and Anthony J. Keller
- Subjects
medicine.medical_specialty ,Immunology ,Blood Donors ,Disclosure ,Viral infection ,Donor Selection ,Interviews as Topic ,Risk Factors ,Internal medicine ,medicine ,Disease Transmission, Infectious ,Prevalence ,Immunology and Allergy ,Humans ,Risk factor ,Disease Notification ,Selection (genetic algorithm) ,business.industry ,Donor selection ,Australia ,Transfusion Reaction ,Hematology ,Hepatitis C ,Hepatitis B ,medicine.disease ,Residual risk ,Virus Diseases ,Viral disease ,business - Abstract
BACKGROUND: Selection of voluntary donors who are at low risk of transfusion-transmissible viral infection (TTVI) is central in maintaining the safety of the blood supply. Evaluation of its effectiveness and the dynamics of the process may offer opportunities to further improve transfusion safety. STUDY DESIGN AND METHODS: The impact of donor selection on prevalence of TTVI was analyzed in all allogeneic donations in Australia between July 2000 and June 2006 by interviewing donors found to have a TTVI. The presence and disclosure of infective risks was reassessed. RESULTS: A total of 6.3 million donations were tested; of these, 1449 (0.02%) were repeat-reactive for a TTVI and were discarded. This comprised 605 (42%) positive for the presence of hepatitis B, 818 (56%) positive for the presence of hepatitis C, 18 (1%) positive for the presence of human immunodeficiency virus, and 20 (1%) positive for the presence of human T-cell lymphotropic virus-I and/or -II (HTLV-I/II). This prevalence was 50 to 350 times lower than in the Australian population. In 1158 cases (80%), an infective risk was identified; 509 donors (44%) had more than one. The most common identified were country of birth and parental ethnicity (n = 682, 26% of risks), tattoos and/or piercings (n = 448, 18%), and intravenous drug use (n = 302, 12%). In 302 cases (21%) disclosure at predonation screening would have resulted in deferral. Factors influencing nondisclosure included temporal remoteness and perceptions that laboratory testing rendered disclosure unnecessary. CONCLUSION: These findings affirm the effectiveness of current stringent donor selection criteria in reducing the residual risk of TTVI. Ongoing donor education regarding the importance of risk disclosure is required. T he freedom of the blood supply from transfusion-transmissible infection (TTI) is protected by a combination of measures, including the use of voluntary, nonremunerated donors; donor education and careful donor selection; and sensitive laboratory screening of donated blood. 1 Together, these measures have resulted in an extremely low residual risk of transfusion-transmitted viral infections (TTVIs). 2
- Published
- 2007
49. Comparison of two automated nucleic acid testing systems for simultaneous detection of human immunodeficiency virus and hepatitis C virus RNA and hepatitis B virus DNA
- Author
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Anthony J. Keller, Philip Kiely, Clive R. Seed, Angelo R. Margaritis, Bruno D'Agostino, and Stewart M. Brown
- Subjects
Hepatitis B virus ,Hepatitis C virus ,Hepacivirus ,Immunology ,Human immunodeficiency virus (HIV) ,medicine.disease_cause ,Virus ,Flaviviridae ,Automation ,medicine ,Immunology and Allergy ,Humans ,Multiplex ,Blood Transfusion ,biology ,Australia ,virus diseases ,HIV ,Hematology ,biology.organism_classification ,Virology ,Molecular biology ,Nat ,Lentivirus ,DNA, Viral ,Hong Kong ,RNA, Viral - Abstract
BACKGROUND: Recently developed nucleic acid testing (NAT) assays incorporating simultaneous detection of human immunodeficiency virus (HIV), hepatitis C virus (HCV), and hepatitis B virus (HBV) have made HBV NAT screening more feasible for blood services. This study compared the performance of two “multiplex” NAT assays and their automated testing platforms. STUDY DESIGN AND METHODS: The HBV NAT yield rate was estimated by testing 10,397 Hong Kong (HK) donor samples concurrently on the PROCLEIX ULTRIO (Ultrio) assay as individual donor samples with the TIGRIS and on the cobas TaqScreen multiplex (cobas MPX) test in pools of 6 with the cobas s 201. Analytical sensitivity was assessed by probit analysis of diluted international standards and operational performance was compared. RESULTS: Each system detected two different HBV NAT yield samples for a combined rate of 0.04 percent. One additional sample was reactive on the cobas MPX test but remained unresolved. The 95 percent detection limits for HIV-1, HBV, and HCV were 42.2, 12.2, and 2.0 IU per mL, respectively, for Ultrio and 50.5, 8.4, and 6.0 IU per mL for the cobas MPX. The invalid test and failed run rates were 0.05 and 2.92 percent, respectively, for the TIGRIS and 2.39 and 5.53 percent for the cobas s 201. CONCLUSION: Clinical sensitivity for HBV in HK blood donors was equivalent, as was the analytical sensitivity for HIV-1 and HBV; however, the Ultrio assay had a higher analytical sensitivity for HCV. Despite a shorter downtime and mean time of repair for the cobas s 201, the TIGRIS demonstrated better overall operational performance.
- Published
- 2007
50. The efficacy of a malarial antibody enzyme immunoassay for establishing the reinstatement status of blood donors potentially exposed to malaria
- Author
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Anthony J. Keller, Anthea Cheng, T. J. Cobain, Timothy M. E. Davis, Clive R. Seed, A. D. Kitchen, and Bolton Wv
- Subjects
Time Factors ,Recombinant antigen ,Plasmodium falciparum ,Antibodies, Protozoan ,Blood Donors ,Risk Assessment ,Sensitivity and Specificity ,Immunoenzyme Techniques ,Medicine ,Animals ,Humans ,Mass Screening ,chemistry.chemical_classification ,medicine.diagnostic_test ,biology ,business.industry ,Incidence (epidemiology) ,Incidence ,Hematology ,General Medicine ,medicine.disease ,biology.organism_classification ,Malaria ,Enzyme ,Blood donor ,chemistry ,Immunoassay ,Immunology ,biology.protein ,Antibody ,business ,Algorithms - Abstract
Background and Objectives The two key objectives of the study were, first, to evaluate the sensitivity and specificity of a recombinant antigen-based malarial enzyme-linked immunoassay (EIA) and, second, to estimate the risk associated with implementing this test with a shortened cellular component restriction period (6 months rather than the standard 12–36 months) for blood donors with a malarial risk exposure. Materials and Methods Blood donors were recruited into four distinct groups [non-exposed (control), malarial area ‘visitors’, ‘residents’ and ‘previous infection’) and screened by using the Newmarket malarial antibody EIA. Assay specificity was evaluated in unexposed blood donors, and sensitivity was determined in acute clinical samples. Results No parasitaemic donors were detected amongst 337 malarial ‘visitors’ who had returned from a malaria-endemic area less than 6 months previously, or for 402 ‘visitors’ or ‘residents’ who had returned from a malaria-endemic area more than 6 months previously. The incidence of malarial antibodies within the exposed blood donor groups was 1·33% (10/751). In acute clinical non-donor samples, the Newmarket EIA detected 106/108 (98·1; 93·5–99·5%) ‘film’ positive Plasmodium falciparum infections and 12/12 (100, 75·7–100·0%) P. vivax infections. The estimated additional risk exposure of the proposed new strategy was one infectious P. falciparum donation per 175 years or 1 per 4·2 years for P. vivax. Conclusions The study findings support the efficacy and safety of a targeted screening strategy combining antibody screening with a 6-month cellular component restriction period for donors with a declared malarial risk.
- Published
- 2005
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