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Internationally representative data of middle-aged adults 45 - 65 years old [n(United States) = 2,150; n(Japan) = 2,318; n(South Korea) = 2,800] from the 2012 Program for International Assessment of Adult Competencies were analyzed to examine the roles of education and literacy in relation to the digital divide. Results from survey-weighted binary logistic regressions showed that both educational attainment and literacy were positively associated with all four measures of information and communication technology (use of the computer, email, online information and transaction) use in all three countries. The middle-aged adults in the United States benefited more from the educational attainment than those in Japan, in terms of email and online information use. The middle-aged adults with lower education and basic skills (i.e., literacy) may benefit from the educational intervention and additional information and communication technology training, and in turn, improve the digital divide in later life, regardless of differences in culture and economy.

THE CORONATION OF King Charles III made for great television: horsemen in breastplates and plumes; a bejeweled aristocracy and the emissaries of empire; a whiff of scandal over the royals [...]

Intrinsically disordered proteins and protein regions (IDPs) are prevalent in all proteomes and are essential to cellular function. Unlike folded proteins, IDPs exist in an ensemble of dissimilar conformations. Despite this structural plasticity, intramolecular interactions create sequence-specific structural biases that determine an IDP ensemble's three-dimensional shape. Such structural biases can be key to IDP function and are often measured in vitro, but whether those biases are preserved inside the cell is unclear. Here we show that structural biases in IDP ensembles found in vitro are recapitulated inside human-derived cells. We further reveal that structural biases can change in a sequence-dependent manner due to changes in the intracellular milieu, subcellular localization, and intramolecular interactions with tethered well-folded domains. We propose that the structural sensitivity of IDP ensembles can be leveraged for biological function, can be the underlying cause of IDP-driven pathology or can be used to design disorder-based biosensors and actuators.

Abstract: The trend of global cooling across the Cenozoic transformed the North American landscape from closed forest to more open grasslands, resulting in dietary adaptations in herbivores in response to shifting resources. In contrast, the material properties of the predator food source (muscle, skin, and bone) have remained constant over this transition, suggesting a corresponding lack of change in predator dietary adaptations. We investigated the North American mammalian predator fossil record using a tooth-shape metric and body mass, predicting that the former would exhibit stability. Instead, we found that mean molar morphology became more blade-like, with our tooth-shape metric sharply increasing in the late Eocene and remaining high from the Oligocene onward. Subsequent tests in extant carnivorans reveal taxa with more bladelike teeth are prevalent in more open environments. Our results reveal an unexpected functional shift among North American predators in response to large-scale environmental changes across the Cenozoic.

The first discovery of any dinosaur remains in a US National Park Service unit in Alaska occurred in 2001 in Aniakchak National Monument and Preserve. The record consisted of the track of a pes impression, or track made by the foot of a hadrosaur (duck-billed dinosaur) and an associated manus impression, or track made by a hand. Subsequent work has shown the original track discovery was not unique, and that the coastal exposures of the Cretaceous Chignik Formation in Aniakchak National Monument and Preserve contain a remarkable number of tracks. Further, because of the limited faulting, the several hundred meters of section found along this coastal set of exposures provide a remarkably complete look at an ancient high-latitude dinosaurian ecosystem and are of outstanding universal value.

Intensive disease surveillance in an endangered population of Ethiopian wolves provided evidence of concurrent outbreaks of rabies and canine distemper viruses in 2019, including co-infection in an individual animal. Disease surveillance and intensive monitoring of wolf packs in Ethiopia were essential in detecting the concurrent outbreaks and enabled accurate assessment of disease from both pathogens. The study highlights the risk posed to endangered populations that are susceptible to, or live in areas with, reservoir hosts for canine distemper and rabies viruses. Instances of concurrent distemper and rabies outbreaks appear unusual in the existing literature; modeling for one disease might underestimate the risk for extinction. Concurrent outbreaks may have a larger effect than single-disease outbreaks, even in a population that has partial vaccination coverage. Researchers studying wildlife populations from a conservation perspective should be aware that both diseases can strike at once where susceptible populations exist.

Abstract Extreme short-duration rainfall is intensifying with climate warming, and growing evidence suggests that subhourly rainfall extremes are increasing faster than more widely studied durations at hourly and daily timescales. In this case study, we used 55 years (1968–2022) of 5-min precipitation data from Mahantango Creek, a long-term experimental agricultural watershed in east-central Pennsylvania, United States, to examine annual and seasonal changes in subhourly (15-min), hourly, and daily rainfall extremes. Specifically, we evaluated temporal trends in the magnitude and frequency of subhourly, hourly, and daily rainfall extremes. We then estimated apparent scaling rates between rainfall extremes and dew point temperature (Td) and compared these rates to the Clausius-Clapeyron (CC) rate (∼ 7% per °C). We also determined the coincidence of extreme rainfall trends with indicators of atmospheric instability and convective-type precipitation. Overall, we found the most significant changes in rainfall extremes at 15-min durations during the spring, with magnitudes of these subhourly extremes increasing by 0.6 to 0.9% per year, and frequencies rising by 3.4% per year. Apparent scaling rates in the spring showed that 15-min rainfall extremes transitioned from sub-CC scaling to greater than 2CC scaling when Td reached 11° C, implying a possible shift from stratiform rains to more intense convective rains above this Td threshold. Notably, trends in maximum hourly convective available potential energy (CAPE) increased during spring, as did the ratio of 15-min rainfall extremes to their corresponding daily rainfall totals. Findings indicate that convective-type precipitation may be playing an increasing role in the intensification of springtime 15-min rainfall extremes in Mahantango Creek.

Over the past two decades, it has become increasingly apparent that early Mars may once have been warmer, wetter and more habitable for microbial life than it is today, which has spurred discussions about potential biosignatures that may be preserved in Martian sediments. An impediment to this line of research is the pervasive oxidation of Mars’ surface due to photochemical oxidants that have likely destroyed remnants of organic matter. Here, we investigate whether nitrogen (N) transferred from biomass to phyllosilicate minerals during diagenesis can be preserved in oxidized mudrocks. We investigate two sequences of terrestrial Proterozoic red beds, namely the Sibley Group (1.4 Ga) in Canada and the Stoer Group (1.2 Ga) in Scotland, and we find enrichments in authigenic N in the range of several tens of ppm in both units. The highest concentrations (ca. 100 ppm on average) are found in the most desiccated red beds of the Stoer Group, concurrent with enrichments in potassium (K). We discuss similarities and differences between the two sets of rocks with regards to salinity, pH, biological productivity and K-metasomatism, and we conclude that the ideal mechanism for the preservation of biogenic N in red beds may be in-situ release of ammonium from microbial mats into the clay substrate, possibly facilitated by early diagenetic, biologically induced illitization. Illite and smectite have been observed on Mars, and experiments suggest that Martian waters contained moderate amounts of dissolved K. Hence, it is conceivable that a similar K and N enrichment process could have occurred as to what we document for the Proterozoic, preserving evidence of life that may have survived to the modern day.

Background: Total elbow arthroplasty (TEA) is an effective surgical intervention that can be used in the treatment of elbow pathologies including osteoarthritis (OA), complex distal humerus fractures, and rheumatoid arthritis (RA). However, there is a paucity of literature assessing trends in the utilization of TEA. The purpose of this study was to identify trends in TEA utilization in the United States (U.S.) from 2010 to 2018. Methods: A query of the IBM Watson Health MarketScan Database was performed to identify patients that underwent TEA from 2010 to 2018 using Current Procedural Terminology and International Classification of Disease coding. Patients were stratified based on surgical indication into the following groups: distal humerus fracture/post-traumatic sequelae, RA, OA, and other. Population estimates from the U.S. Census Bureau were used to estimate the annual incidence and procedural trends of primary TEA for each surgical indication. Further stratification evaluated TEA trends based on sex, age, and geographic region. Results: A total of 6522 primary TEAs were performed between 2010 and 2018. The total annual volume of TEAs performed from the start to the end of this time period decreased by 33%, from 694 to 466 cases. Overall, the majority (53.9%, n = 3514) of all TEAs from 2010 to 2018 were performed to treat distal humerus fractures/post-traumatic sequelae, while 22.3% (n = 1457) were performed for RA, 10.8% (n = 702) for OA, and 13.0% (n = 849) for other. Volume and incidence of TEA decreased over time from 2010 to 2018 regardless of surgical indication, sex, and age. The greatest decreases in volume and incidence in TEAs during the study period was observed for RA (58% and 60%, respectively). The smallest change in volume and incidence of TEA was observed for OA, with a 9% decrease in volume from 57 to 52 cases and 14% decrease in incidence from 0.19 to 0.16 per 1,000,000 people. Conclusions: The incidence and volume of primary TEAs in the U.S. decreased from 2010 to 2018, regardless of surgical indication, sex, and age. The most common indication for TEA is distal humerus fractures and traumatic sequelae, and RA is now a much less common indication than it was in the past. Understanding the national trends of TEA utilization allows us to visualize changes in practice over time to highlight preferences in the treatment of various elbow pathologies.

Objective: A less-invasive left atrial assist device (LAADx) is a novel and implantable, extracardiac blood pump concept, intended for the treatment of diastolic heart failure, represented by heart failure with preserved ejection fraction. Methods: A mixed-flow pump was used as the working LAADx model. Its performance was evaluated at 3 speeds, using an in vitro pulsatile mock circulatory loop, with a pneumatic pump that can simulate diastolic heart failure conditions by adjusting the diastolic drive pressure. The LAADx model was implanted in 4 healthy calves. The pump's inflow and outflow cannulas were inserted into the left atrium (LA) and left ventricle (LV), respectively, without cardiopulmonary bypass. The LAADx was operated at 3 speeds, and diastolic heart failure-like conditions were induced by inflating a balloon, inserted into the LV. Results: With the in vitro study, diastolic heart failure-like conditions were successfully induced, exhibiting decreased cardiac output and aortic pressure as well as increased mean LA pressure both mitigated with the LAADx support. With regard to the in vivo study, simulated diastolic heart failure conditions showed a decrease in aortic pressure and an increase in LA pressure and LV end-diastolic pressure, which were again mitigated by the LAADx. Echocardiography showed good positioning of the outflow cannula and neither cardiac dysfunction nor mitral interference was observed. Conclusions: Initial in vitro and in vivo results confirmed that the LAADx model, a device concept driven by creating an extracardiac route from the LA to LV, has the potential to mitigate high LA pressure and improve LV filling of heart failure with preserved ejection fraction pathology.

Abstract PIK3CA-related overgrowth spectrum (PROS) disorders are caused by somatic mosaic variants that result in constitutive activation of the phosphatidylinositol-3-kinase/AKT/mTOR pathway. Promising responses to molecularly targeted therapy have been reported, although identification of an appropriate agent can be hampered by the mosaic nature and corresponding low variant allele frequency of the causal variant. Moreover, our understanding of the molecular consequences of these variants—for example how they affect gene expression profiles—remains limited. Here we describe in vitro expansion of a human capillary malformation followed by molecular characterization using exome sequencing, single cell gene expression, and targeted long-read single cell RNA-sequencing in a patient with clinical features consistent with Megalencephaly-Capillary Malformation Syndrome (MCAP, a PROS condition). These approaches identified a targetable PIK3CA variant with expression restricted to PAX3+ fibroblast and undifferentiated keratinocyte populations. This study highlights the innovative combination of next-generation single cell sequencing methods to better understand unique transcriptomic profiles and cell types associated with MCAP, revealing molecular intricacies of this genetic syndrome.

Abstract Forests harbor extensive biodiversity and act as a strong global carbon and nitrogen sink. Although enhancing tree diversity has been shown to mitigate climate change by sequestering more carbon and nitrogen in biomass and soils in manipulative experiments, it is still unknown how varying environmental gradients, such as gradients in resource availability, mediate the effects of tree diversity on carbon and nitrogen accrual in natural forests. Here, we use Canada’s National Forest Inventory data to explore how the relationships between tree diversity and the accumulation of carbon and nitrogen in tree biomass and soils vary with resource availability and environmental stressors in natural forests. We find that the positive relationship between tree functional diversity (rather than species richness) and the accumulation of carbon in tree biomass strengthens with increasing light and soil nutrient availability. Moreover, the positive relationship between tree functional diversity and the accumulation of carbon and nitrogen in both organic and mineral soil horizons is more pronounced at sites with greater water and nutrient availabilities. Our results highlight that conserving and promoting functionally diverse forests in resource-rich environments could play a greater role than in resource-poor environments in enhancing carbon and nitrogen sequestration in Canada’s forests.