1. Topical anti-inflammatory potential of Physalin E from Physalis angulata on experimental dermatitis in mice
- Author
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Cecília Rocha da Silva, Talita Cavalcante Morais, Natália Bitu Pinto, Otília Deusdênia L. Pessoa, Flávia A. Santos, Gerly Anne de Castro Brito, Vietla Satyanarayana Rao, Geanne Matos de Andrade, Karine Maria Martins Bezerra Carvalho, and Maria Leopoldina Veras
- Subjects
Male ,Physalis ,Anti-Inflammatory Agents ,Pharmaceutical Science ,Dermatite ,Physalis angulata ,Inflammation ,Pharmacology ,Dermatitis, Contact ,Oxazolone ,Mice ,chemistry.chemical_compound ,Drug Discovery ,Animals ,Secosteroids ,Medicine ,Antígeno Polipeptídico Tecidual ,Dexamethasone ,Ratos ,biology ,business.industry ,Antagonist ,Histology ,Mifepristone ,biology.organism_classification ,Immunohistochemistry ,Complementary and alternative medicine ,chemistry ,Myeloperoxidase ,Immunology ,biology.protein ,Tetradecanoylphorbol Acetate ,Molecular Medicine ,medicine.symptom ,business ,medicine.drug - Abstract
The anti-inflammatory effect of physalin E, a seco-steroid isolated from Physalis angulata L. was evaluated on acute and chronic models of dermatitis induced by 12-O-tetradecanoyl-phorbol-13-acetate (TPA) and oxazolone, respectively, in mouse ear. The changes in ear edema/thickness, production of pro-inflammatory cytokines (TNF-alpha and IFN-gamma), myeloperoxidase (MPO) activity, and histological and immunohistochemical findings were analysed, as indicators of dermal inflammation. Similar to dexamethasone, topically applied Physalin E (0.125; 0.25 and 0.5 mg/ear) potently inhibited the TPA and oxazolone-induced dermatitis, leading to substantial reductions in ear edema/thickness, pro-inflammatory cytokines, and MPO activity. These effects were reversed by mifepristone, a steroid antagonist and confirmed by immunohistochemical and histopathological analysis. The data suggest that physalin E may be a potent and topically effective anti-inflammatory agent useful to treat the acute and chronic skin inflammatory conditions.
- Published
- 2010
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