Search

Your search keyword '"Anne Katrine Steffensen"' showing total 4 results
Start Over Author "Anne Katrine Steffensen"
4 results on '"Anne Katrine Steffensen"'

1. Breakthrough infections with the omicron and delta variants of SARS-CoV-2 result in similar re-activation of vaccine-induced immunity

Author

Arne Søraas, Gunnveig Grødeland, Beathe Kiland Granerud, Thor Ueland, Andreas Lind, Børre Fevang, Sarah L. Murphy, Camilla Huse, Anders Benteson Nygaard, Anne Katrine Steffensen, Huda al-Baldawi, Mona Holberg-Petersen, Lise Lima Andresen, Camilla Ågnes, Trine Ranheim, Ylva Schanke, Mette Istre, John Arne Dahl, Adity Chopra, Susanne Dudman, Mari Kaarbø, Jan Terje Andersen, Eline Benno Vaage, Trung The Tran, John Torgils Vaage, Annika E. Michelsen, Fredrik Müller, Pål Aukrust, Bente Halvorsen, Tuva B. Dahl, Jan Cato Holter, and Fridtjof Lund-Johansen

Subjects
vaccine, SARS-CoV-2, human, antibody, Breakthrough infection, cellular immunity, Immunologic diseases. Allergy, RC581-607
Abstract

BackgroundResults showing that sera from double vaccinated individuals have minimal neutralizing activity against Omicron have been interpreted as indicating the need for a third vaccine dose for protection. However, there is little information about early immune responses to Omicron infection in double vaccinated individuals.MethodsWe measured inflammatory mediators, antibodies to the SARS-CoV-2 spike and nucleocapsid proteins, and spike peptide-induced release of interferon gamma in whole blood in 51 double-vaccinated individuals infected with Omicron, in 14 infected with Delta, and in 18 healthy controls. The median time points for the first and second samples were 7 and 14 days after symptom onset, respectively.FindingsInfection with Omicron or Delta led to a rapid and similar increase in antibodies to the receptor-binding domain (RBD) of Omicron protein and spike peptide-induced interferon gamma in whole blood. Both the Omicron- and the Delta-infected patients had a mild and transient increase in inflammatory parameters.InterpretationThe results suggest that two vaccine doses are sufficient to mount a rapid and potent immune response upon infection in healthy individuals of with the Omicron variant.FundingThe study was funded by the Oslo University Hospital, and by grants from The Coalition for Epidemic Preparedness Innovations, Research Council of Norway (no 312780, 324272), South-Eastern Norway Regional Health Authority (no 2019067, 2021071, 10357, 2021047, 33612, 2021087, 2017092), EU Horizon 2020 grant no 848099, a philantropic donation from Vivaldi Invest A/S, and The European Virus Archive Global.

Published
2022
Full Text
View/download PDF

2. Omicron Variant Generates a Higher and More Sustained Viral Load in Nasopharynx and Saliva Than the Delta Variant of SARS-CoV-2

Author

Beathe K. Granerud, Thor Ueland, Andreas Lind, Arne Søraas, Børre Fevang, Anne Katrine Steffensen, Huda Al-Baldawi, Fridtjof Lund-Johansen, Pål Aukrust, Bente Halvorsen, Tuva B. Dahl, Susanne Dudman, Fredrik Müller, and Jan Cato Holter

Subjects
viral load, Omicron variant, Delta variant, Norway, SARS-CoV-2, Microbiology, QR1-502
Abstract

The Omicron variant of SARS-CoV-2 spreads more easily than earlier variants, possibly as a result of a higher viral load in the upper respiratory tract and oral cavity. Hence, we investigated whether the Omicron variant generates a higher viral load than that of the Delta variant in saliva and nasopharynx. Both specimens were collected from 52 Omicron and 17 Delta cases at two time points one week apart and analyzed by qRT-PCR. Viral load was measured as 10 log RNA genome copies per 1000 human cells according to the WHO reference standard. We found that Omicron cases carried a higher viral load and had more sustained viral shedding compared to the Delta cases, especially in the nasopharynx.

Published
2022
Full Text
View/download PDF

3. Multicenter Evaluation of Rapid BACpro® II for the Accurate Identification of Microorganisms Directly from Blood Cultures Using MALDI-TOF MS

Author

Gilbert Greub, Anne Katrine Steffensen, Belén Rodríguez-Sánchez, David Rodriguez-Temporal, André Ingebretsen, Germán Bou, Lidia Quiroga, Antony Croxatto, Marina Oviaño, and Guy Prod'hom

Subjects
MALDI-TOF, Medicine (General), Gram-negative bacteria, Chromatography, biology, medicine.diagnostic_test, Chemistry, Microorganism, Gram-positive bacteria, Clinical Biochemistry, Gold standard (test), rapid BACpro® II kit, mass spectrometry, blood culture, rapid identification, biology.organism_classification, Matrix-assisted laser desorption/ionization, R5-920, Species level, medicine, Blood culture, Gram
Abstract

The identification of microorganisms directly from blood cultures using MALDI-TOF MS has been shown to be the most impacting application of this methodology. In this study, a novel commercial method was evaluated in four clinical microbiology laboratories. Positive blood culture samples (n = 801) were processed using a rapid BACpro® II kit and then compared with the routine gold standard. A subset of monomicrobial BCs (n = 560) were analyzed in parallel with a Sepsityper® Kit (Bruker Daltonics, Bremen, Germany) and compared with the rapid BACpro® II kit. In addition, this kit was also compared with two different in-house methods. Overall, 80.0% of the monomicrobial isolates (609/761; 95% CI 71.5–88.5) were correctly identified by the rapid BACpro® II kit at the species level (92.3% of the Gram negative and 72.4% of the Gram positive bacteria). The comparison with the Sepsityper® Kit showed that the rapid BACpro® II kit generated higher rates of correct species-level identification for all categories (p > 0.0001), except for yeasts identified with score values > 1.7. It also proved superior to the ammonium chloride method (p > 0.0001), but the differential centrifugation method allowed for higher rates of correct identification for Gram negative bacteria (p > 0.1). The percentage of accurate species-level identification of Gram positive bacteria was particularly noteworthy in comparison with other commercial and in-house methods.

Published
2021
Full Text
View/download PDF

4. Evaluation of the rapidBACpro® II kit for the rapid identification of microorganisms directly from blood cultures using MALDI-TOF MS

Author

Lidia Quiroga, Germán Bou, Belén Rodríguez-Sánchez, André Ingebretsen, Guy Prod'hom, Anne Katrine Steffensen, Marina Oviaño, Antony Croxatto, and Gilbert Greub

Subjects
Gram-negative bacteria, Chromatography, biology, Chemistry, Gram-positive bacteria, Microorganism, Gold standard (test), biology.organism_classification, medicine.disease, Rapid identification, Matrix-assisted laser desorption/ionization, Bacteremia, medicine, Gram
Abstract

ObjectivesIdentification of microorganisms directly from blood cultures (BCs) using MALDI-TOF MS has shown to be the application with most impact in this methodology. In this study, a novel commercial method, the rapidBACpro® II, was evaluated in four clinical microbiology laboratories.MethodsPositive blood culture samples (n=801) were processed using the rapidBACpro® II kit and then compared with routine gold standard. A subset of monomicrobial BCs (n=560) were analyzed in parallel with the Sepsityper® kit (Bruker Daltonics, Bremen, Germany) and compared with the rapidBACpro® II kit. In addition, the rapidBACpro® II kit was also compared with two different in-house methods.ResultsOverall, 80.0% of the monomicrobial isolates (609/761) were correctly identified by the rapidBACpro® II kit at the species level (92.3% of the Gram negative and 72.4% of the Gram positive bacteria). The comparison with the Sepsityper® kit yielded higher rates of correct species-level identification provided by the rapidBACpro® II kit for all categories (p>0.0001) except for yeasts identified with score values >1.7. It also proved superior to the ammonium chloride method (p>0.0001) but the differential centrifugation method allowed higher rates of correct identification for Gram negative bacteria (p>0.1).ConclusionsThe rapidBACpro® II kit allowed a high rate of microorganisms correctly identified. The percentage of accurate species-level identification of Gram positive bacteria was particularly noteworthy in comparison with other commercial and in-house methods. This fact was especially interesting in the case of Staphylococcus sp. and Streptococcus sp. in order to elucidate their clinical impact, for example in device-associated bacteremia.

Published
2021
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results