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The carbon footprint is an index used to assess the impact of an activity in terms of greenhouse gas emissions. Viticulture contributes to greenhouse gas emissions due to the use of fuels, fertilizers and pesticides, and the consequent soil erosion. Organic viticulture differs from conventional viticulture, mainly because of the absence of synthetic products, the soil tillage, and the level of organic carbon in the soil. The purpose of the study was to determine the actual differences between conventional and organic vineyard management in terms of greenhouse gas emissions, comparing multiannual data from 25 wineries in northern Italy. No statistically significant differences were found between the overall mean values of conventional and organic management. In organically farmed vineyards, a higher incidence of fuel consumption was observed, while in conventionally farmed vineyards higher emissions were observed, due to the use of such products as pesticides and fertilizers. No differences were found between the two management systems in terms of emissions resulting from direct fertilizing. Further assessment of the potential sequestration of organic fertilizer would be necessary.

Popillia japonica, a priority pest for the EU, was first detected in Northern Italy in 2014. Since its discovery, the outbreak extended over an area of more than 16,000 square kilometers in Northern Italy and Southern Switzerland. In this review, we summarize the state-of-the-art of research conducted in Italy on both the spreading capacity and control measures of P. japonica. Chemical, physical, and biological control measures deployed since its detection are presented, by highlighting their strengths and weaknesses. An in-depth study of the ecosystems invaded by P. japonica disclosed the presence and pathogenicity of natural strains of entomopathogenic fungi and nematodes, some of which have shown to be particularly aggressive towards the larvae of this pest under laboratory conditions. The Plant Health authorities of the Lombardy and Piedmont regions, with the support of several research institutions, played a crucial role in the initial eradication attempt and subsequently in containing the spread of P. japonica. Control measures were performed in the infested area to suppress adult populations of P. japonica by installing several traps (e.g., for mass trapping, for auto-dissemination of the fungus Metarhizium anisopliae, and “attract & kill”). For larval control, the infested fields were treated with commercial strains of the entomopathogenic fungus M. anisopliae and nematode Heterorhabditis bacteriophora. Future studies will aim at integrating phenological and spread models developed with the most effective control measures, within an ecologically sustainable approach.

Background: Recent studies indicated that frequent anthropogenic disturbances in tropical countries are primary drivers of the reduction in community diversity and local extinction of many insect taxa, including Lepidoptera. Biodiversity assessment provides information on the responses of different taxa to these anthropogenic changes, useful to develop evidence-based conservation strategies to reduce biodiversity loss. We assessed the impact of anthropogenic disturbances on lepidopteran assemblages across five different land uses (forest fragments, crop fields, Pasture land, rural settlement and undisturbed natural forest) in Choke Mountains, Ethiopia. Automatic light traps have been used in 16 sites, in 3 sites manual samplings have been performed. A total of 4,559 moths representing 14 families and 339 species were sampled. The highest diversity was obtained from the Natural Forest (269 species), followed by Forest Fragment (174), Pasture Land (148), Crop Fields (138), and Rural Settlement (136). The highest species-rich families were Geometridae (131 taxa), Erebidae (93 taxa), Tortricidae (40 taxa) and Sphingidae (17 taxa). Values calculated for the Shannon index were high, ranging between 4.78 for Natural Forests and 4.15 for Crop Fields. Simpson index indicated very high values of evenness, with all land use types above 0.97. The highest values of dominance were observed in Crop Field and Pasture Land, with the percentage of the dominant species around 10%. Estimated species richness by Chao-1 was highest within Natural Forest (366), followed by Pasture Land (256), Forest Fragment (241), Rural Settlement (233), and lowest in Crop Field (186), with the highest increase, if compared to the observed number of species, in Pasture Land (+ 73%) and Rural Settlement (+ 71%). Conclusions: The results of this study will be useful for guiding conservation management priorities for preventing irreversible biodiversity losses and maintaining ecosystem services provisioning that are essential conditions for the sustainable development of rural communities.

Financiado para publicación en acceso aberto: Universidade de Vigo/CISUG In this work we study from the mathematical and numerical point of view a problem arising in vector-borne plant diseases. The model is written as a nonlinear system composed of a parabolic partial differential equation for the vector abundance function and a first-order ordinary differential equation for the plant health function. An existence and uniqueness result is proved using backward finite differences, uniform estimates and passing to the limit. The regularity of the solution is also obtained. Then, using the finite element method and the implicit Euler scheme, fully discrete approximations are introduced. A discrete stability property and a main a priori error estimates result are proved using a discrete version of Gronwall’s lemma and some estimates on the different approaches. Finally, some numerical results, in one and two dimensions, are presented to demonstrate the accuracy of the approximation and the behaviour of the solution. Agencia Estatal de Investigación | Ref. PGC2018-096696-B-I00

The fall armyworm, Spodoptera frugiperda (J.E. Smith), is an invasive pest threatening crop production and food security worldwide. High concerns are linked to the potential establishment of the species in Europe. The high migratory capacity of S. frugiperda cause concerns about the potential impacts of transient populations invading new areas from suitable hotspots. In the present work, we developed and used a physiologically-based demographic model to quantitatively assess the risks of S. frugiperda in Europe. The risks were assessed considering a best-, a median-, and a worst-case scenario. The Mediterranean coastal areas of Southern Europe resulted suitable for the establishment of the species, with suitable areas reaching even higher latitudes, in the worst-case scenario. In Europe, up to 4 generations per year were predicted. The predicted average yearly adult population was 5 (± 4), 17 (± 5), and 139 (± 22) in the best, median-, and worst-case assessment scenario, respectively. Model results showed that Southern and Central Europe might be exposed to the risk of transient populations, at least up to the 48th parallel north, in the worst-case scenario. Depending on the latitude and on the period of arrival of the propagule, transient populations might be able to originate 1 or more generations per year. The model can be used to define strategies reducing the risks of establishment of the pest at the country level. Predictions on the dynamics and phenology of the pest can also be used to support its management at the local level.

Food Frequency Questionnaires (FFQs) are valuable research tools in nutritional epidemiology. This study aimed to develop and validate a new semi-quantitative FFQ, specifically designed for the Italian population and best fitted for self-administration. During the development process, we adapted to Italian needs the validated FFQ proposed by the Fred Hutchinson Cancer Research Center, revising food items, food frequency scale, portion sizes, and time frame. To assess the validity of the proposed FFQ, we compared the estimated daily intake using FFQ with the mean of 3-day food diaries and one 24-hour recall (considered as reference method). The validation process was conducted among a cohort of 51 healthy subjects enrolled in a clinical trial. Four statistical tests were applied on 23 estimated nutrient intakes. Spearman's coefficients ranged from 0.223 (sodium) to 0.748 (alcohol) and were good (≥0.50) and acceptable (0.20-0.49) for 7 and 16 nutrients, respectively. Cross classification showed a good agreement (≥50% in the same tertile or ≤10% in the opposite tertile) for 7 nutrients. The weighted Cohen's kappa values indicated an acceptable outcome (0.20-0.60) for 13 nutrients. Bland Altman plots did not show heteroscedasticity in the error terms, despite the presence of a bias. Our study provided a new Italian semi-quantitative FFQ for self-administration with an acceptable validation level. Its definitive release requires additional refinements and efforts.

Information technology clearly influences the decision-making behaviour of individuals, groups, and organisations. In particular, social media and Web 2.0 technologies can affect the rationality and effectiveness of the policy cycle in the public sector. This growing influence deserves to be analysed. This work aims to understand how the influence of social media in the different phases of the policy cycle is perceived by mayors, the main decision-makers in local governments.

The fall armyworm,Spodoptera frugiperda(J.E. Smith), is an invasive pest threatening crop production and food security worldwide. High concerns are linked to the potential establishment of the species in Europe. The high migratory capacity ofS. frugiperdacauses concerns about the potential impacts of transient populations invading new areas from suitable hotspots. In the present work, we developed and used a physiologically-based demographic model to quantitatively assess the risks ofS. frugiperdain Europe. The risks were assessed considering a best-, a median-, and a worst-case scenario. The Mediterranean coastal areas of Southern Europe resulted particularly suitable for the establishment of the species, with suitable areas reaching even higher latitudes, in the worst-case scenario. In Europe, up to four generations per year were predicted. The predicted yearly average number of moths per trap per week (± standard deviation) was 5 (± 4), 17 (± 5), and 139 (± 22) in the best, median-, and worst-case assessment scenarios, respectively. Model results showed that Southern and Central Europe up to the 48th parallel north might be exposed to the risk of transient populations. Depending on the latitude and on the period of arrival of the propagule, 1–2 transient generations per year might be expected. The model can be used to define strategies for reducing the risks of establishment of the pest at the country level. Predictions on the dynamics and phenology of the pest can also be used to support its management at the local level.

This final report evaluates the training courses delivered under the contract NP/EFSA/SCER/2018/01. The courses are to explain the basics of how to conduct environmental risk assessments (ERA’s) with a particular focus on (1) living organisms, i.e. the deliberate commercial release into the environment of Genetically Modified Organisms (GMO’s), or the introduction and spread of invasive alien species that are harmful to plant health; and (2) substances used as pesticides or as feed additives (FA).A total of six training courses were organised, which were attended by in total 113 participants. The first four trainings were organised in 2019 and they took place at the European Food Safety Authority (EFSA) in Parma. The last two trainings were organised as webinar training courses.The targeted participants of the training courses were members of EFSA’s Scientific Committee/Panels and their working groups, preferably the newly designated ones. Members of the EFSA Networks as well as EFSA scientific staff could also participate in the training courses.The delivered training courses were perceived by the participants as good to very good.

Vineyard is a multifunctional ecosystem associated to a multitude of environmental benefits and ecosystem services. Despite the increased research efforts on the analysis of biodiversity patterns and ecosystem services in vineyard, the lack of approaches to multifunctionality suggests to develop integrated approaches allowing to manage the complexity of vineyard landscape. The present study introduces an innovative methodology aimed at developing a unitary framework able to describe vineyard ecosystems biodiversity considering both local (morphological characteristics, internal ecological infrastructure, and management) and landscape (land-use) components. The case of Franciacorta wine-growing area is described considering a total of 112 ha located in three wine companies. Four informative layers related to different components of Franciacorta territory were defined: i) morphology; ii) internal ecological infrastructure; iii) landscape composition; iv) management. This study provides a useful instrument to increase knowledge about vineyard system biodiversity and to protect ecosystem services provision in Franciacorta territory. The methodology applied can easily be extended to the whole wine-growing compartment.

We deve loped a mechanistic, stage-structured model simulating the phenology of Popillia japonica. The model simulates the influence of soil temperature on the larval diapause termination and on the development rate function of post-overwintering larvae and pupae. Model parameters are estimated based on literature evidence for pupae development and on a parameterisation process that allows estimating parameters for larval diapause termination and for the development rate function (and the related uncertainty) of post-overwintering larvae. Data used for model parameterisation and validation refer to time-series adult trap catches collected during the P. japonica monitoring programme performed by the Phytosanitary Service of Lombardy Region within the infested area in Lombardy (Italy) from 2015 to 2019. A total of 12 randomly selected locations are used to estimate biologically realistic model parameters (parameterisation dataset). We applied a Jackknife nonparametric resampling procedure on the parameterisation dataset to quantify uncertainty associated with parameters’ estimates. Parameterised model is then validated on time-series adult trap catches data referring to a different set of 12 randomly selected locations (validation dataset) surveyed in Lombardy. The model successfully predicted the beginning of adult emergence and the overall curve of adult emergence in the validation dataset. The model presented can support the definition of the best timing for the implementation of monitoring and control activities for the local and the area-wide management of P. japonica.

Honey bees play an important role in the maintenance of both, biodiversity and food security through pollination services and also represent an important source of income for rural areas. Despite several studies and monitoring projects gathering a large amount of data on the main factors/stressors influencing honey bee colonies, there is a lack of holistic and multidimensional statistical tools integrating different aspects which define honey bee colony health. Such tools are important to support the sustainable management of honey bees. In this study, we designed and tested a methodological framework based on Structural Equation Models for the development of a honey bee Health Status Index. The index accounts for the main abiotic (e.g. pesticide contamination, landscape characteristics) and biotic (e.g. parasitosis, virosis) factors influencing honey bee health, including the beekeepers role in managing the colony. The proposed methodology was validated against a dataset representing seven scenarios generated through Expert Knowledge Elicitation. The validation procedure showed that the Health Status Index integrates different sources of data and it can quantify the health status of a honey bee colony based on the characteristics of each scenario. Furthermore, it allows the investigation of the influence of different drivers/stressors on the health of the honey bee colony. The Health Status Index provides flexibility in the selection of variables, making it a valuable tool for holistic and harmonised assessment of honey bee health. Once validated, the index can support the evaluation at different spatial (from local- to area-wide management) and temporal (medium- to long-term management) scales to support stakeholders’ (e.g. beekeepers, risk assessors, risk managers) decision-making.

The meadow spittlebug Philaenus spumarius plays the major role in the spread of X. fastidiosa subsp. pauca ST53 on olives in the Apulia region (Italy). Despite the vector’s movement is a key component of X. fastidiosa epidemiology, little information is available. Dispersal capabilities of the meadow spittlebug were hence investigated by mark-release-recapture (MRR) experiments carried out from May to October in olive grove and meadow, in the Apulia and the Piedmont regions (Italy), respectively. Field-collected adults of P. spumarius were marked with an aqueous solution of albumin (10%) and released at a single point in the centre of the experimental area. A total of ≈6500 and ≈3000 spittlebugs were released during Apulia and Piedmont experiments, respectively. Spittlebugs were recaptured with sweep net (10–250 m distance from release point) every two or three days up to 17 days after the release. Marked insects were identified via an indirect ELISA. The dispersal capacity of P. spumarius adults was described by estimating the dispersal kernel, i.e. the probability density function describing the distribution of the recapture locations of insects relative to the release point. Spittlebugs were recaptured up to 155 meters and 200 m in Apulia and Piedmont, with 50% of total recaptures within 30 m and 40 m from the release points, respectively. Results showed a high variability in the estimated median distance from the release point, ranging from 26 m (Apulia) to 35 m (Piedmont) per day, and from 374 m (Apulia) to 507 m (Piedmont) for adult life-long dispersion. Estimated spread parameters of P. spumarius are fundamental to model X. fastidiosa spread in Apulia and other European foci. Further research is needed to investigate the influence of insect physiological status and environmental factors on movement and directionality of P. spumarius., IT; PPT; nicola.bodino@ipsp.cnr.it

The introduction of the Xylella fastidiosa Wells bacterium into Apulia (South Italy) has caused the massive dieback of olive trees, and is threatening olive production throughout the Mediterranean Region. The key vector of X. fastidiosa in Europe is the spittlebug Philaenus spumarius L. The dispersal capabilities of P. spumarius are poorly known, despite being a key parameter for the prediction of the spread of the bacterium. In this study, we have examined the dispersal of P. spumarius adults in two different agroecosystems in Italy: an olive grove in Apulia (Southern Italy) and a meadow in Piedmont (Northern Italy). Insects were marked with albumin and released during seven independent trials over 2 yr. The recapture data were pooled separately for each agroecosystem and used to estimate the dispersal kernels of P. spumarius in the olive grove and in the meadow. The diffusion coefficient estimate for P. spumarius was higher in the meadow than in the olive grove. The median distance from the release point for 1 d of dispersal was 26 m in the olive grove and 35 m in the meadow. On the basis of our model, we estimated that 50% of the spittlebug population remained within 200 m (98% within 400 m) during the 2 mo period of high abundance of the vector on olives in Apulia. The dispersal of P. spumarius is thus limited to some hundreds of meters throughout the whole year, although it can be influenced to a great extent by the structure of the agroecosystem.

Simple Summary Spittlebugs are the most abundant and widespread xylem-sap feeder insects in Europe. They are also the only proven vectors of the notorious bacterium Xylella fastidiosa (Xf) in the Old World. Xf inhabits the xylem of hundreds of plant species and is the causal agent of severe diseases to several crop plants, including grapevines. Since the spread of Xf depends on insect transmission, the study of vector abundance and ecology in the vineyard is of key importance in assessing the risk of disease spread to grapevines. The aim of this work was to gain information on Xf vector ecology and abundance in the vineyard agroecosystem. Herbaceous cover of inter-rows and headlands was colonized in spring by nymphs and in late summer/autumn by adults for oviposition, while woody hosts (grapevines and trees in the surroundings) represented a refuge during summer. Three spittlebug species were collected: Philaenus spumarius, Neophilaenus campestris, and Aphrophora alni, but the latter two species were very rare on the grapevine canopy. The presence of P. spumarius for an extended period on the grapevine canopy, together with its known ability to transmit Xf to grapevine, underlines the importance of preventing the introduction of Xf in Xf-free areas and of monitoring health conditions of grapevines in the Xf-infected areas of Europe. Abstract Spittlebugs (Hemiptera: Aphrophoridae) are the vectors of the bacterium Xylella fastidiosa (Xf) in Europe. Xf may cause severe epidemics in cultivated plants, including grapevines. To assess the threat represented by the bacterium to grapevines, detailed information on the vectors’ phenology, density, and ecology in vineyards is needed. The aim of the present work was to describe spittlebug diversity, phenology, and host-plant association in the vineyard agroecosystem. Two separate field surveys of nymphal and adult spittlebug populations, i.e., a two-year survey of a single site and a one-year survey of three sites, were performed in vineyards of northwestern Italy in three consecutive years. Philaenus spumarius was the most common species, reaching average nymph densities on herbaceous cover up to 60–130 nymphs/m2. Adults were sampled on grapevines from May to September, with a peak in June (up to 0.43 insects/sweep). Herbaceous cover was colonized after egg hatching and in late summer for oviposition, while wild woody hosts represented a refuge during summer. The results show that spittlebugs can reach high population levels in vineyards, at least in the areas where the ground is covered by herbaceous plants for the whole season and the use of insecticides is moderate. The extended presence of P. spumarius adults on grapevines represents a serious risk factor for the spread of Xf. The scenarios of Xf establishment in vineyards in northwestern Italy and Europe are discussed in relation to the abundance, phenology, and plant association of spittlebugs.

The importance of soil biodiversity and soil-based ecosystem services in the context of viticulture has recently been emphasized. Over 85% of soil fauna species richness is represented by edaphic arthropod communities. Edaphic arthropod responses to soil characteristics and management practices can be considered as good bioindicators of soil quality. Here, 168 soil samples that were collected from 2014 to 2019 in several vineyards of different Italian wine-growing areas were analyzed to explore how arthropod communities respond to several factors that are characteristic of vineyard ecosystems. The analysis of the combined effects of the primary abiotic variables (the chemical and physical characteristics of soil) and management practices (organic vs. conventional, soil inter-row management) on soil biological quality (assessed by QBS-ar index) identified soil temperature and soil texture as the abiotic factors exerting the most significant effect on the QBS-ar values. Organic vineyards exhibited higher QBS-ar values compared to those of conventionally managed vineyards, and subsoiling negatively influenced the soil biological quality.

Soil represents an important pool of biodiversity, hosting about a quarter of the living species on our planet. This soil richness has led to increasing interest in the structural and functional characteristics of its biodiversity. Studies of arthropod responses, in terms of abundance and taxon richness, have increased in relation to their ecological value as bioindicators of environmental change. This research was carried out over the 2014&ndash, 2018 period with the aim to better understand arthropod taxa responses in vineyard soils in Franciacorta (Lombardy, Italy). To determine the biological composition in terms of arthropod taxa presence, one hundred soil samples were analysed. Environmental characteristics, such as chemical composition, soil moisture and temperature, and soil management were characterized for each soil sample. A total of 19 taxa were identified, the NMDS model analysis and the cluster analysis divided them into five groups according to their co-occurrence patterns. Each group was related to certain abiotic conditions, of these, soil moisture, temperature and organic matter were shown to be significant. A decision tree analysis showed that a longer period since conversion from conventional to organic farming lead to a higher arthropod biodiversity defined as a higher number of taxa.

The concept of the water-energy-food (W-E-F) nexus has quickly ascended to become a global framing for resource management policies. Critical studies, however, are questioning its value for assessing the sustainability of local livelihoods. These critiques flow in part from the perception that the majority of influential nexus analyses begin from a large-scale, implicitly top-down perspective on resource dynamics. This can lead to efficiency narratives that reinforce existing power dynamics without adequate consideration of local priorities. Here, we present a community-scale perspective on large W-E-F oriented infrastructure. In doing so, we link the current debate on the nexus with alternative approaches to embrace questions of water distribution, political scales, and resource management. The data for this paper come from a survey of 549 households conducted around two large-scale irrigation and hydropower dams in the Upper Blue Nile basin of Ethiopia. The data analysis involved descriptive statistics, logistic analysis, and multinomial logistic analysis. The two case studies presented show that the impact of dams and the perception thereof is socially diverse. Hydropower dams and irrigation schemes tend to enhance social differences and may therefore lead to social transformation and disintegration. This becomes critical when it leads to higher vulnerability of some groups. To take these social factors/conditions into consideration, one needs to acknowledge the science-policy interface and make the nexus approach more political. The paper concludes that if the nexus approach is to live up to its promise of addressing sustainable development goals by protecting the livelihoods of vulnerable populations, it has to be applied in a manner that addresses the underlying causes that produce winners and losers in large-scale water infrastructure developments.

Spittlebugs are the vectors of the bacterium Xylella fastidiosa Wells in Europe, the causal agent of olive dieback epidemic in Apulia, Italy. Selection and distribution of different spittlebug species on host-plants were investigated during field surveys in 2016&ndash, 2018 in four olive orchards of Apulia and Liguria Regions of Italy. The nymphal population in the herbaceous cover was estimated using quadrat samplings. Adults were collected by sweeping net on three different vegetational components: herbaceous cover, olive canopy, and wild woody plants. Three species of spittlebugs were collected: Philaenus spumarius L., Neophilaenus campestris (Fall&eacute, n), and Aphrophora alni (L.) (Hemiptera: Aphrophoridae). Philaenus spumarius was the predominant species both in Apulia and Liguria olive groves. Nymphal stages are highly polyphagous, selecting preferentially Asteraceae Fabaceae plant families, in particular some genera, e.g., Picris, Crepis, Sonchus, Bellis, Cichorium, and Medicago. Host-plant preference of nymphs varies according to the Region and through time and nymphal instar. In the monitored sites, adults peak on olive trees earlier in Apulia (i.e., during inflorescence emergence) than in Liguria (i.e., during flowering and beginning of fruit development). Principal alternative woody hosts are Quercus spp. and Pistacia spp. Knowledge concerning plant selection and ecological traits of spittlebugs in different Mediterranean olive production areas is needed to design effective and precise control strategies against X. fastidiosa vectors in olive groves, such as ground cover modifications to reduce populations of spittlebug vectors.

Background. To review currently available evidence on the effect of cow-milk proteins supplementation (CPS) on health in the elderly. Methods. Five electronic databases (Pubmed, Web of Science, Embase, Cochrane Library, ClinicalTrials.gov) were searched for studies about CPS among older people. All types of publications were included, with the exception of systematic reviews, meta-analyses, opinion letters, editorials, case reports, conference abstracts and comments. An additional search in Google Scholar and a manual review of the reference lists were performed. Results. Overall, 103 studies were included. Several studies explored the role of CPS in the preservation or improvement of muscle mass among healthy subjects (40 studies) and pre-frail, frail or sarcopenic patients (14), with evidence of beneficial effects. Other studies assessed the effect of CPS on bones (12), cardiovascular disease (8), inflamm-aging (7), chronic pulmonary disease (4), neurocognitive function (4), and vaccines (2), with weak evidence of positive effects. Seven studies in the field of protein metabolism investigated the role of CPS as an important contributor to nutritional needs. Other investigational areas are considered in the last five studies. Conclusions. The beneficial effects of CPS in achieving aged-related nutritional goals, in preserving muscle mass and in recovering after hospitalization may be particularly relevant in the elderly.

Aims: The aim of this study was to assess the effects of leaf removal and bunch shading on the analytical composition of Pinot noir and Chardonnay (Vitis vinifera L.) grapes suitable for making premium sparkling wine.Method and results: Total bunch defoliation (TD) and different treatments using shading nets (TD1L, TD2L and ND1L) were evaluated in comparison with a test with no defoliation and shading (ND) over three seasons in the southern part of Franciacorta, one of the most famous Italian sparkling wine regions. Micrometeorological variables, yield components, musts and grapes chemical composition were evaluated. Shading practices lead to a delay in ripening and they improve the acidic content of must, thus resulting in a potential improvement in the quality of juice suitable for producing sparkling wines. Furthermore, this particular type of vine canopy management leads to changes in the phenolic content of grapes.Conclusions: From the results obtained it was possible to underline the positive effect - delaying ripening, preserving acid concentration and reducing flavonol content - of shading on the composition of Pinot noir and Chardonnay grapes suitable for making premium sparkling wine.Significance of the study: This study shows the importance of shading, because it delays grape ripening and thereby preserves the acidic content of musts and, specifically, deals with the problem of early ripening related to the climate change now underway.

Purpose The purpose of this paper is to study the effects of mandatory inter-municipal cooperation (IMC) in small Italian municipalities. Data from 280 small Italian municipalities on the effects of IMC in terms of higher efficiency, better effectiveness of local public services, and greater institutional legitimacy of the small municipalities participating in IMC have been investigated against four variables: size, geographical area, type of inter-municipal integration, and IMC membership (the presence in the IMC of a bigger municipality, the so-called big brother). Design/methodology/approach Data were gathered from a mail survey that was sent to a random sample of 1,360 chief financial officers acting in municipalities of under 5,000 inhabitants, stratified by size (0-1,000 and 1,001-5,000) and geographic area (North, Center, and South) criteria. To analyze the dependency relationships between the three potential effects of participating in IMC and possible explanatory variables, the authors used a logistic regression model as the benefits were binarily categorized (presence or absence of benefits). Findings The findings show that in more than two-thirds of the municipalities participating in IMC, there were benefits in terms of costs reduction and better public services, whereas greater institutional legitimacy was detected in about half of the cases. The statistical analysis with logistic regression highlighted that IMC type is particularly critical for explaining successful IMC. In particular, the positive effects of IMC were mainly detected in those small municipalities that promoted a service delivery organization rather than participating in service delivery agreements or opting for mixed arrangements of joint public services delivery. Originality/value The paper focuses on small municipalities where studies are usually scant. The analysis highlighted that the organizational setting is particularly critical for explaining a successful IMC.

Honey bee colonies are fundamental for the provision of goods and ecosystem services. Honey bees are highly influenced by environmental conditions and quality, beekeepers’ management practices, socio-economic conditions and policies adopted for cropping and land use. We propose a modelling framework aiming at assessing the bee health status and forecast colony outputs. Two modelling tools are here presented: (i) a Health Status Index (HSI) exploring the consequences of abiotic, biotic drivers and beekeeping actions on bee health; and (ii) predictive models for the estimation of honey production and the provision of pollination service considering abiotic, biotic drivers and HSI. The models proposed represent useful tools for science-based decision support for beekeepers, risk managers and policymakers.

Spittlebugs (Hemiptera: Aphrophoridae) are the dominant xylem-sap feeders in the Mediterranean area and the only proven vectors of Xylella fastidiosa ST53, the causal agent of the olive dieback epidemic in Apulia, Italy. We have investigated the structured population phenology, abundance and seasonal movement between crops and wild plant species of both the nymphal and adult stages of different spittlebug species in olive groves. Field surveys were conducted during the 2016–2018 period in four olive orchards located in coastal and inland areas in the Apulia and Liguria regions in Italy. The nymphal population in the herbaceous cover was estimated using quadrat samplings. Adults were collected through sweep nets on three different vegetational components: herbaceous cover, olive canopy and wild woody plants. Philaenus spumarius was the most abundant species; its nymphs were collected from early March and reached a peak around mid-April, when the 4th instar was prevalent. Spittlebug adults were collected from late April until late autumn. P. spumarius adults were abundant on the herbaceous cover and olive trees in late spring, and they then dispersed to wild woody hosts during the summer and returned to the olive groves in autumn when searching for oviposition sites in the herbaceous cover. A relatively high abundance of P. spumarius was observed on olive trees during summer in the Liguria Region. The present work provides a large amount of data on the life cycle of spittlebugs within an olive agroecosystem that can be used to design effective control programmes against these vectors in infected areas and to assess the risk of the establishment and spread of X. fastidiosa to Xylella-free areas.

Biodiversity is an increasingly important aspect of wine production. The assessment of agro-ecosystem biodiversity is highly complex due to the heterogeneity of the elements involved in the evaluation. For this reason, wine companies have expressed a need for a decision support system (DSS) capable of dealing with this complexity, integrating assessments referring to the whole production system within a single tool. In this study a DSS developed for wine sector biodiversity management assessment is introduced. The DSS, called BIOPASS&reg, is made up of different sections relating to three compartments in the winemaking process (the soil, the vine and wine). Assessment of the physical, chemical and biological components of soil is a key element of the DSS. We investigate the relationship between biological soil quality (represented by the QBS-ar index), environmental conditions and the type of farming (organic or conventional). 70 soil samples were analysed in different Italian viticultural contexts. The model highlighted the relationships between QBS-ar and meteorological variables (air temperature and precipitation) as well as a positive relationship with organic farming systems. These results provide useful information for understanding agroecosystem biodiversity and will be integrated within the DSS for assessment of soil quality.

The beekeeper plays a key role in maintaining the health status of managed honey bee colonies and ensuring their productivity. However, a clear overview on the main actions carried out by beekeepers and their role for the successful management of honey bees is only partially addressed. In this study, we aim at providing: i) a generalized conceptual framework for the characterization of the Beekeeping Management Practices carried out by the European beekeepers and ii) the definition of their influence on the overall status of the honey bee colony. Six Beekeeping Management Practices were selected in this study: chemical control; replacement of combs with brood; replacement of combs with feed sources; supplementary feeding; change in the number of workers; beekeeper category and experience. Each Beekeeping Management Practice was characterized in relation to: i) the elements guiding their application, ii) the potential impacts on a honey bee colony and iii) the scenario-based variables that might influence their timing, frequency and/or efficiency. We performed an extensive literature review and an Expert Knowledge Elicitation procedure in order to estimate the uncertainty linked to some major parameters. In this paper, we successfully developed and applied a conceptual framework defining the actions carried out by European beekeepers and quantifying their impacts to a honey bee colony. The conceptual framework might support the definition of realistic scenarios of Beekeeping Management Practices in Europe for the assessment and management of the risks linked to honey bee colonies considering the potential role of the beekeeper.

The rational control of harmful organisms for plants (pests) forms the basis of the integrated pest management (IPM), and is fundamental for ensuring agricultural productivity while maintaining economic and environmental sustainability. The high level of complexity of the decision processes linked to IPM requires careful evaluations, both economic and environmental, considering benefits and costs associated with a management action. Plant protection models and other decision tools (DTs) have assumed a key role in supporting decision-making process in pest management. The advantages of using DTs in IPM are linked to their capacity to process and analyze complex information and to provide outputs supporting the decision-making process. Nowadays, several DTs have been developed, tackling different issues, and have been applied in different climatic conditions and agricultural contexts. However, their use in crop management is restricted to only certain areas and/or to a limited group of users. In this paper, we review the current state-of-the-art related to DTs for IPM, investigate the main modelling approaches used, and the different fields of application. We also identify key drivers influencing their adoption and provide a set of critical success factors to guide the development and facilitate the adoption of DTs in crop protection.

This paper focuses on the measurement of human capital, specifically on the growth of cognitive skills (CS) during the higher education at university. CS have already been evaluated in a macroeconomic perspective inside the neoclassical growth models, but not still in a micro perspective. However the measure of educational quality and learning process is still an issue not fully addressed. The micro approach allows the researcher to focus on the evaluation of CS acquisition process. Based on these measurements, different types of CS accumulation can be identified. We will investigate CS through nonlinear latent growth modeling, and we will apply this methodology to administrative data of an Italian university.

Smoking in pregnancy increases asthma risk in offspring and possibly in grand-offspring. Few studies have examined the effect of paternal smoking on offspring9s health although smoking in male adolescence may cause transmittable epigenetic changes in sperm precursor cells. Furthermore, the statistical evaluation of such transgenerational effects is challenging. We assessed the effect of smoking in different susceptibility periods (mother/grandmother9s pregnancies, father9s adolescence) on asthma risk in offspring by a three-generation analysis. At the ECRHS III clinical interview (2010-2013), 2140 mothers and 1866 fathers (level 2 units) from 26 centres (cluster variable) reported whether their offspring (aged ≤51 years; level 1 units) had Asthma and whether it coexisted with allergic Rhinitis (A+R) or not (A–R). These parents also provided information on their parents9 and their own asthma/schooling/smoking history. Two-level 2→2→1 mediation models with cluster-robust standard errors (outcome: offspring9s A+R/A–R; mediator: parent9s ever asthma) were fitted separately within the maternal (4451 offspring) and paternal (3984 offspring) lines. Grandmother9s smoking in pregnancy was associated with mother9s ever asthma (OR=1.51; 95%CI: 1.13-2.02) and with grand-offspring9s A+R within the maternal line (relative risk ratio, RRR=1.36; 95%CI: 1.12-1.65). Mother9s smoking in pregnancy (RRR=1.26; 95%CI: 1.01-1.56) and father9s smoking in adolescence (RRR=1.48; 95%CI: 1.02-2.13) were associated with offspring9s A–R. Parental and grandparental smoking in different susceptibility periods may increase asthma risk across generations. This analysis is part of the ALEC Study (EU Horizon 2020 Grant N° 633212).

PURPOSE To analyze the results of hyperopic patients treated with a peripheral presbyLASIK algorithm for the correction of presbyopia. METHODS The study included 44 eyes of 22 hyperopic patients treated with a peripheral presbyLASIK technique using a Technolas 217z excimer laser. Mean patient age was 56 years (range: 47 to 72 years), mean preoperative spherical equivalent refraction was +1.21±0.77 diopters (D) (range: +0.50 to +4.00 D), and mean spectacle near addition was 1.76±0.42 D (range: +1.00 to +2.75 D). The Peripheral Multifocal LASIK (PML) ablation pattern creates a multifocal corneal profile over a 6.5-mm diameter, performing the distance correction first in a 6-mm optical zone and then near correction in a 6.5-mm zone. Main outcome measures were uncorrected visual acuity (UCVA) and best spectacle-corrected visual acuity (BSCVA) for near and distance, spherical equivalent refraction, contrast sensitivity, and corneal aberrations. RESULTS Six months postoperatively, mean binocular UCVA was 1.06±0.13 for distance and 0.84±0.14 for near. Mean postoperative spherical equivalent refraction was –0.42 D (range: –1.12 to +0.87 D). Two (4.5%) eyes lost 1 line of BSCVA for distance and near vision, and 20 (45%) eyes gained 1 line of distance BSCVA. Contrast sensitivity decreased for 3, 6, 12, and 18 cycles/degree. Corneal aberration analysis showed a slight increase in coma and decrease in spherical aberration. CONCLUSIONS The peripheral presbyLASIK technique used in this study is a safe and efficient treatment that may improve functional near vision in presbyopic patients with low and moderate hyperopia (from +0.50 to +3.00 D). [J Refract Surg. 2008;24:494-500.] ABOUT THE AUTHORS From the Istituto Laser Microchirurgia Oculare, Brescia, Italy (Pinelli, Simonetto, Bacchi); the Research and Development Department, Vissum-Instituto Oftalmológico de Alicante, Alicante, Spain (Ortiz, Sala, Alió); and the Ophthalmology Department, Miguel Hernández University, Alicante, Spain (Alió). This study was supported in part by a grant of the Spanish Ministry of Health, Instituto Carlos III, Red Temática de Investigación en Oftalmologia, Subproyecto de Cirugia Refractiva y Calidad Visual (C03/13) and by a grant from the Spanish Generalitat Valenciana, ref: Grupos05/036 Grants and Support for scientific research and technological development in the Comunidad Valenciana for the year 2005. Dr Pinelli has a proprietary interest in the PML software. The remaining authors have no proprietary interest in the materials presented herein. Correspondence: Jorge L. Alió, MD, PhD, Research and Investigation Department, Instituto Oftalmológico Alicante, Vissum Corporation, Avda Denia s/n, Edificio Vissum, 03016 Alicante, Spain. Tel: 34 902 333 344; Fax: 34 965 160 468; E-mail: jlalio@vissum.com Received: September 13, 2006 Accepted: March 16, 2007 Posted online: October 31, 2007