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Continuous, non-invasive, and objective methods to detect flock-level responses to stressors, including intestinal health deviations, are currently lacking in poultry. This proof of principle study investigated the potential of manure odor profiling in monitoring stress responses in Lohmann Brown Classic, Lohmann LSL Classic and Lohmann LSL Lite layer pullets. Stressors were represented by a Salmonella vaccination given to the pullets at wk 3 of age (Dataset 1 and Dataset 2) and wk 16 of age (Dataset 4) and a viral/bacterial vaccine cocktail given at wk 12 of age (Dataset 3). Fresh manure was sampled daily, from 2 days before vaccination to 4 days after vaccination, with 4 pooled manure samples per day per dataset. Manure volatiles were concentrated into stainless steel sorbent tubes and analyzed by a thermal desorption system coupled with gas chromatography-mass spectrometry. Dataset, breed and sample location did not affect the manure volatile profiles before vaccination. Age did affect the manure volatile profiles, with beta-camphor, (Z)-6-Tridecene and serinol peak intensities elevated in young pullets and carbonyl sulfide and trimethylamine elevated in older pullets. Salmonella vaccination at wk 3 of age led to the most consistent changes in volatile peak intensities. (Z)-6-Tridecene was decreased in Dataset 1, Dataset 3, and Dataset 4 after either the Salmonella vaccination or the viral/bacterial vaccine cocktail, despite differences in hen age and house. The injected viral/bacterial vaccine cocktail created a more clear time-dependent shift in the manure volatile profile than the orally-provided Salmonella vaccination in older pullets at resp. wk 12 and 16 of age. No overlapping trends in upregulated or downregulated volatiles were found between all datasets. To conclude, volatile profiles of rearing hen manure are affected by vaccinations as a proxy for stressors, and the magnitude and direction of the response depends on the age of the pullets at vaccination, the vaccination method, and the pathogenic properties of the vaccine. The reduced peak intensities of volatiles after vaccination in 3 out of 4 flocks suggests the potential of manure odor profiling in monitoring stress responses in layer pullets.

ABSTRACT: Daily farm management practices play an essential role in determining and steering health, welfare and productivity of laying hen flocks. Optimal management requires expertise of farmers and coworkers, especially when hens are kept in complex, large-scale aviary systems. Relatively little sensor-based support is available to farmers, even though numerous research groups are working on developing technologies to continuously detect deviations in layer health and welfare. A survey with laying hen farmers, poultry veterinarians and poultry experts from Western Europe and Canada was conducted to identify and prioritize indicators of optimal and suboptimal laying hen health and welfare in commercial farms. The status-quo of sensor technology and the advantages, wishes, and concerns regarding sensors were additionally assessed to contribute to the future development of a predictive monitoring tool that continuously monitors laying hen health and welfare. A total of 45 stakeholders were interviewed, of which 41 filled in an online questionnaire. Although the prioritization of indicators differed between stakeholders, the majority identified the use of feed and water intake, egg production and quality, sound, activity, and movement of hens as important indicators to assess health and welfare. Currently collected (sensor) data were not used to their full potential, and stakeholders missed the integration and storage of data into one monitoring system with easy visualization tools. Most interest was observed in the use of cameras and microphones to detect deviations in health and welfare at an early stage, to reduce subjectivity of the assessment and to gain more knowledge on layer behavior. It can be concluded that these results could steer research efforts towards the development of continuous monitoring techniques, and enhance their adaptability and acceptability by stakeholders.

Contact dermatitis, both on the foot pads and hocks, is a well-known health issue in broilers. Less is known about contact dermatitis in broiler breeders, however, although they have many risk factors for developing leg health problems in common with broilers. This study aimed to describe the prevalence and severity of contact dermatitis during the production cycle in 5 lines of broiler breeders, investigate possible causes of contact dermatitis, and study its relationship with gait, egg production, and floor egg percentage. Five commercially available genetic lines of broiler breeders were housed in 21 pens of 550 females and 50 males from 20 to 60 wk of age. Every 10 wk litter quality, leg health measurements (foot pad dermatitis, hock burn, and gait) and body weight were assessed of 50 random hens per pen. Total number of eggs, number of eggs laid outside the nest (floor eggs), and mortality were recorded daily per pen. Prevalence of foot pad dermatitis, hock burn, and gait problems increased with age. Litter quality started to decrease at 50 wk of age. Prevalence of foot pad dermatitis was affected by litter quality, whereas genetic line had little effect. One genetic line was more prone to developing hock burns, though generally the prevalence of hock burn (13%) was much lower than that of foot pad dermatitis (74%). The percentage of broiler breeders with gait problems increased up to 24% with age, but this was not related to the prevalence of contact dermatitis. The lines differed in body weight from 32 wk of age onwards, and a higher body weight was related to lower egg production and higher cumulative mortality. The percentage of floor eggs was not related to leg health parameters or genetic line. Broiler breeders thus have similar leg health problems as broilers, but these problems are not related to the percentage of floor eggs, suggesting that other factors are involved in the undesirable behavior of floor laying.