17 results on '"Ann E. Green"'
Search Results
2. Building the DDI
- Author
-
Ann E. Green and Chuck Humphrey
- Subjects
Social Sciences - Abstract
Building the DDI
- Published
- 2014
- Full Text
- View/download PDF
3. CD-ROM and the Data Archive: Beyond Retrieval
- Author
-
Ann E. Green
- Subjects
Social Sciences - Abstract
CD-ROM and the Data Archive: Beyond Retrieval
- Published
- 1988
- Full Text
- View/download PDF
4. Organizing a Data Library
- Author
-
Ann E. Green
- Subjects
Social Sciences - Abstract
Organizing a Data Library
- Published
- 1985
- Full Text
- View/download PDF
5. Proteomic analysis of ovarian cancer cells reveals dynamic processes of protein secretion and shedding of extra-cellular domains.
- Author
-
Vitor M Faça, Aviva P Ventura, Mathew P Fitzgibbon, Sandra R Pereira-Faça, Sharon J Pitteri, Ann E Green, Renee C Ireton, Qing Zhang, Hong Wang, Kathy C O'Briant, Charles W Drescher, Michèl Schummer, Martin W McIntosh, Beatrice S Knudsen, and Samir M Hanash
- Subjects
Medicine ,Science - Abstract
BACKGROUND: Elucidation of the repertoire of secreted and cell surface proteins of tumor cells is relevant to molecular diagnostics, tumor imaging and targeted therapies. We have characterized the cell surface proteome and the proteins released into the extra-cellular milieu of three ovarian cancer cell lines, CaOV3, OVCAR3 and ES2 and of ovarian tumor cells enriched from ascites fluid. METHODOLOGY AND FINDINGS: To differentiate proteins released into the media from protein constituents of media utilized for culture, cells were grown in the presence of [(13)C]-labeled lysine. A biotinylation-based approach was used to capture cell surface associated proteins. Our general experimental strategy consisted of fractionation of proteins from individual compartments followed by proteolytic digestion and LC-MS/MS analysis. In total, some 6,400 proteins were identified with high confidence across all specimens and fractions. CONCLUSIONS AND SIGNIFICANCE: Protein profiles of the cell lines had substantial similarity to the profiles of human ovarian cancer cells from ascites fluid and included protein markers known to be associated with ovarian cancer. Proteomic analysis indicated extensive shedding from extra-cellular domains of proteins expressed on the cell surface, and remarkably high secretion rates for some proteins (nanograms per million cells per hour). Cell surface and secreted proteins identified by in-depth proteomic profiling of ovarian cancer cells may provide new targets for diagnosis and therapy.
- Published
- 2008
- Full Text
- View/download PDF
6. 'THE QUALITY OF LIGHT'
- Author
-
Ann E. Green
- Published
- 2017
7. Building the DDI
- Author
-
Chuck Humphrey and Ann E. Green
- Subjects
Metadata ,World Wide Web ,Data Documentation Initiative ,Engineering ,Process management ,Process (engineering) ,business.industry ,Context (language use) ,Set (psychology) ,business - Abstract
This paper describes the context, motivation, and requirements behind the design and development of the first version of the Data Documentation Initiative 2 (DDI) metadata community specification, with an emphasis upon the process of creating the initial element set for the “study level” of DDI version 1. We also offer a framework for understanding the infrastructural changes that contributed to the establishment of the DDI. By taking a close look at the confluence of influences on the earliest efforts to design and build the DDI, we can better understand what essential elements of metadata are necessary to support independent use of social science data over time.
- Published
- 2014
8. ESRRA-C11orf20 is a recurrent gene fusion in serous ovarian carcinoma
- Author
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Julie S. Nielsen, Ann E. Green, Julia Salzman, Brad H. Nelson, Charles W. Drescher, Peter L. Wang, Patrick O. Brown, and Robert J. Marinelli
- Subjects
Oncogene Proteins, Fusion ,Carcinoma, Ovarian Epithelial ,Fusion gene ,Exon ,0302 clinical medicine ,Ovarian carcinoma ,Prevalence ,Copy-number variation ,Neoplasms, Glandular and Epithelial ,Biology (General) ,Genetics ,Ovarian Neoplasms ,0303 health sciences ,General Neuroscience ,Exons ,Genomics ,3. Good health ,Serous fluid ,Receptors, Estrogen ,Oncology ,030220 oncology & carcinogenesis ,Medicine ,Female ,General Agricultural and Biological Sciences ,Research Article ,Canada ,DNA Copy Number Variations ,QH301-705.5 ,Molecular Sequence Data ,Biology ,General Biochemistry, Genetics and Molecular Biology ,Deep sequencing ,03 medical and health sciences ,medicine ,Biomarkers, Tumor ,Humans ,Amino Acid Sequence ,RNA, Messenger ,030304 developmental biology ,Neoplasm Staging ,Chromosome Aberrations ,General Immunology and Microbiology ,Sequence Analysis, RNA ,Chromosomes, Human, Pair 11 ,Computational Biology ,Sequence Analysis, DNA ,medicine.disease ,United States ,Cystadenocarcinoma, Serous ,Alternative Splicing ,Fusion transcript ,Case-Control Studies ,Ovarian cancer - Abstract
Many ovarian cancers have a chromosomal rearrangement that fuses two neighboring genes, ESRRA and c11orf20. Similar rearrangements may be common, important features of cancer genomes that have largely escaped detection., Every year, ovarian cancer kills approximately 14,000 women in the United States and more than 140,000 women worldwide. Most of these deaths are caused by tumors of the serous histological type, which is rarely diagnosed before it has disseminated. By deep paired-end sequencing of mRNA from serous ovarian cancers, followed by deep sequencing of the corresponding genomic region, we identified a recurrent fusion transcript. The fusion transcript joins the 5′ exons of ESRRA, encoding a ligand-independent member of the nuclear-hormone receptor superfamily, to the 3′ exons of C11orf20, a conserved but uncharacterized gene located immediately upstream of ESRRA in the reference genome. To estimate the prevalence of the fusion, we tested 67 cases of serous ovarian cancer by RT-PCR and sequencing and confirmed its presence in 10 of these. Targeted resequencing of the corresponding genomic region from two fusion-positive tumor samples identified a nearly clonal chromosomal rearrangement positioning ESRRA upstream of C11orf20 in one tumor, and evidence of local copy number variation in the ESRRA locus in the second tumor. We hypothesize that the recurrent novel fusion transcript may play a role in pathogenesis of a substantial fraction of serous ovarian cancers and could provide a molecular marker for detection of the cancer. Gene fusions involving adjacent or nearby genes can readily escape detection but may play important roles in the development and progression of cancer., Author Summary Serous ovarian cancer, the most common form of ovarian cancer, is especially lethal because it is usually only detected at a late stage in its progression, after the cancer has spread to other tissues. We searched for molecular markers of this cancer that might provide a better way to detect tumors at a curable stage and that might provide targets for new treatments. Chromosomal rearrangements that fuse two genes to produce a recombinant gene that enhances growth or spread of the cancer are particularly specific biomarkers and have been found in many cancers. By “deep” sequencing of the RNA molecules that carry genetic information in serous ovarian cancers, we discovered a rearrangement that fuses the same two neighboring genes in at least 15% of these tumors. The two fused genes are ESRRA, which encodes a key regulator of gene expression, and an essentially uncharacterized gene, C11orf20, that is normally adjacent to the ESRRA gene. Chromosomal rearrangements that recombine parts of two nearby genes or even parts of a single gene may be a common, important feature of the cancer genome that eludes detection by most approaches to characterizing cancer genomes.
- Published
- 2011
9. Activation of the MEK-S6 pathway in high-grade ovarian cancers
- Author
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Nicole Urban, Ann E. Green, Charles W. Drescher, Beatrice S. Knudsen, Aviva P. Ventura, Kathy O'Briant, Sunitha K. Rajaram, April Allen, Michèl Schummer, Sabarinath Radhakrishnan, Beth Y. Karlan, and Muneesh Tewari
- Subjects
MAPK/ERK pathway ,endocrine system ,Histology ,Blotting, Western ,Antineoplastic Agents ,Biology ,Article ,Pathology and Forensic Medicine ,Phosphatidylinositol 3-Kinases ,Predictive Value of Tests ,Cell Line, Tumor ,medicine ,Biomarkers, Tumor ,Humans ,Phosphorylation ,Protein kinase B ,Protein Kinase Inhibitors ,PI3K/AKT/mTOR pathway ,Phosphoinositide-3 Kinase Inhibitors ,Ovarian Neoplasms ,Kinase ,Ribosomal Protein S6 Kinases ,Ovary ,Ascites ,medicine.disease ,MAP Kinase Kinase Kinases ,Immunohistochemistry ,Up-Regulation ,Medical Laboratory Technology ,Cancer cell ,Cancer research ,Female ,Ovarian cancer ,Proto-Oncogene Proteins c-akt ,Signal Transduction - Abstract
The primary objective of this study is to demonstrate the activation and analyze the regulation of the MEK- S6 kinase pathway in high-grade ovarian cancer. Phospho-ERK (pERK), a direct substrate of MEK and two phosphorylation sites on the ribosomal protein, S6, Ser235/236 and Ser240/244, which are both targeted by the MEK and PI3-kinase/AKT pathways, were analyzed in 13 cell lines, 28 primary cancers and 8 cases of cancer cells from ascites. In primary cancers, ERK and S6 phosphorylation was measured by immunohistochemistry (IHC). pERK, pS6, pAKT and p4EBP1 were also measured by Western blotting (WB). The regulation of S6 phosphorylation by the MEK and PI3-kinase pathways was determined in ovarian cancer cell lines. We observed frequent pERK expression in primary ovarian cancers (100 % by WB, 75% by IHC) but not in ovarian cancer cells from ascites (25% of cases by WB). The activation of the AKT pathway, measured by pAKT expression occurred in 7 cases of primary ovarian cancer by WB, but in none of the ascites samples. In ovarian cancer cell lines, the MEK pathway had a greater effect on S6 phosphorylation in cells without hyperactive AKT signaling. Our data suggest that MEK is a potential drug target in high-grade ovarian cancer, however cancer cells with hyperactive AKT and cancer cells in ascites may be less responsive to MEK inhibition. The phosphorylation of S6 as a specific biomarker for either MEK or PI3-kinase pathway activation should be used with caution.
- Published
- 2010
10. Comparison of Breast Cancer to Healthy Control Tissue Discovers Novel Markers with Potential for Prognosis and Early Detection
- Author
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Martin W. McIntosh, Michèl Schummer, Nicole Urban, Jenny Gross, J. David Beatty, Beth Y. Karlan, Scott Karlan, Ann E. Green, Sean Thornton, and Borgmann, Kerstin
- Subjects
Oncology ,Pathology ,medicine.medical_treatment ,lcsh:Medicine ,law.invention ,0302 clinical medicine ,law ,80 and over ,2.1 Biological and endogenous factors ,Cluster Analysis ,Breast ,Aetiology ,Young adult ,lcsh:Science ,Polymerase chain reaction ,Cancer ,Oligonucleotide Array Sequence Analysis ,Aged, 80 and over ,screening and diagnosis ,0303 health sciences ,Tumor ,Multidisciplinary ,Reverse Transcriptase Polymerase Chain Reaction ,Genetics and Genomics/Gene Expression ,Middle Aged ,Prognosis ,3. Good health ,Detection ,Real-time polymerase chain reaction ,030220 oncology & carcinogenesis ,Mammaplasty ,Genetics and Genomics/Gene Discovery ,Female ,Biotechnology ,Research Article ,Adult ,medicine.medical_specialty ,General Science & Technology ,Breast Neoplasms ,Biology ,03 medical and health sciences ,Young Adult ,Rare Diseases ,Breast cancer ,Internal medicine ,Breast Cancer ,Genetics ,medicine ,Biomarkers, Tumor ,Humans ,Genetic Predisposition to Disease ,Genetics and Genomics/Cancer Genetics ,Aged ,030304 developmental biology ,Prevention ,Gene Expression Profiling ,lcsh:R ,Histology ,medicine.disease ,4.1 Discovery and preclinical testing of markers and technologies ,Gene expression profiling ,Early Diagnosis ,lcsh:Q ,Biomarkers - Abstract
This study was initiated to identify biomarkers with potential value for the early detection of poor-outcome breast cancer. Two sets of well-characterized tissues were utilized: one from breast cancer patients with favorable vs. poor outcome and the other from healthy women undergoing reduction mammaplasty. Over 46 differentially expressed genes were identified from a large list of potential targets by a) mining publicly available expression data (identifying 134 genes for quantitative PCR) and b) utilizing a commercial PCR array. Three genes show elevated expression in cancers with poor outcome and low expression in all other tissues, warranting further investigation as potential blood markers for early detection of cancers with poor outcome. Twelve genes showed lower expression in cancers with poor outcome than in cancers with favorable outcome but no differential expression between aggressive cancers and most healthy controls. These genes are more likely to be useful as prognostic tissue markers than as serum markers for early detection of aggressive disease. As a secondary finding was that, when histologically normal breast tissue was removed from a distant site in a breast with cancer, 7 of 38 specimens displayed a cancer-like expression profile, while the remaining 31 were genetically similar to the reduction mammaplasty control group. This finding suggests that some regions of ipsilateral histologically 'normal' breast tissue are predisposed to becoming malignant and that normal-appearing tissue with malignant signature might warrant treatment to prevent new primary tumors. © 2010 Schummer et al.
- Published
- 2010
11. Proteomic Analysis of Ovarian Cancer Cells Reveals Dynamic Processes of Protein Secretion and Shedding of Extra-Cellular Domains
- Author
-
Samir M. Hanash, Mathew P. Fitzgibbon, Qing Zhang, Hong Wang, Beatrice S. Knudsen, Martin W. McIntosh, Reneé C. Ireton, Vitor M. Faça, Kathy O'Briant, Michèl Schummer, Sandra R. Pereira-Faca, Sharon J. Pitteri, Charles W. Drescher, Ann E. Green, and Aviva P. Ventura
- Subjects
Proteomics ,Cell ,lcsh:Medicine ,Biology ,Ovarian tumor ,Tandem Mass Spectrometry ,Cell Line, Tumor ,medicine ,Humans ,Secretion ,lcsh:Science ,Ovarian Neoplasms ,Multidisciplinary ,Proteomic Profiling ,lcsh:R ,Proteolytic enzymes ,medicine.disease ,Molecular biology ,Cell biology ,Neoplasm Proteins ,medicine.anatomical_structure ,Proteome ,Oncology/Gynecological Cancers ,lcsh:Q ,Biotechnology/Protein Chemistry and Proteomics ,Female ,Ovarian cancer ,Extracellular Space ,Research Article ,Chromatography, Liquid - Abstract
Background Elucidation of the repertoire of secreted and cell surface proteins of tumor cells is relevant to molecular diagnostics, tumor imaging and targeted therapies. We have characterized the cell surface proteome and the proteins released into the extra-cellular milieu of three ovarian cancer cell lines, CaOV3, OVCAR3 and ES2 and of ovarian tumor cells enriched from ascites fluid. Methodology and Findings To differentiate proteins released into the media from protein constituents of media utilized for culture, cells were grown in the presence of [13C]-labeled lysine. A biotinylation-based approach was used to capture cell surface associated proteins. Our general experimental strategy consisted of fractionation of proteins from individual compartments followed by proteolytic digestion and LC-MS/MS analysis. In total, some 6,400 proteins were identified with high confidence across all specimens and fractions. Conclusions and Significance Protein profiles of the cell lines had substantial similarity to the profiles of human ovarian cancer cells from ascites fluid and included protein markers known to be associated with ovarian cancer. Proteomic analysis indicated extensive shedding from extra-cellular domains of proteins expressed on the cell surface, and remarkably high secretion rates for some proteins (nanograms per million cells per hour). Cell surface and secreted proteins identified by in-depth proteomic profiling of ovarian cancer cells may provide new targets for diagnosis and therapy.
- Published
- 2008
12. Difficult Stories: Service-Learning, Race, Class, and Whiteness
- Author
-
Ann E. Green
- Subjects
Critical consciousness ,Class (computer programming) ,Literature and Literary Theory ,Service-learning ,Subject (philosophy) ,Human sexuality ,Pragmatics ,Language and Linguistics ,Education ,Epistemology ,Scholarship ,Pedagogy ,Sociology ,Composition (language) - Abstract
AL s service-learning scholarship enters its second generation, the writing on service-learningl must begin to reflect our own-and our institutions'complex relationship to "doing good." Since service-learning is a widely accepted part of many college curriculums, those who write about servicelearning must go beyond the pragmatics of when and how to integrate service into composition courses and begin to theorize who participates in servicelearning programs and why they do so. I hope, as Cynthia Rosenberger writes, that service-learning can create a "more just and humane society," and believe that in order to do this service-learning must "generate a thoughtful and critical consciousness in all stakeholders" (39). We must begin theorizing how service-learning is experienced differently by those from different groups and look closely at the gaps between our theories of service-learning and our theories of subject position(s), of race, class, gender, sexuality, and writing. Recent work
- Published
- 2003
13. Learning to Tell Stories: Social Class, Narratives, and Pedagogy
- Author
-
Ann E. Green
- Subjects
Political science ,Pedagogy ,Ocean Engineering ,Gender studies ,Narrative ,Social class ,Social pedagogy - Published
- 2003
14. Off the Radar Screen: Gender, Adjuncting, and Teaching Institutions
- Author
-
Ann E. Green, Cecilia Ready, and Susan Naomi Bernstein
- Subjects
White (horse) ,Literature and Literary Theory ,Multimedia ,media_common.quotation_subject ,Field (Bourdieu) ,Subject (philosophy) ,Gender studies ,computer.software_genre ,Language and Linguistics ,Education ,Scholarship ,Argument ,Rhetoric ,Sociology ,Discipline ,computer ,Composition (language) ,media_common - Abstract
When we read Michael Murphy's article, "New Faculty for a New University," we were surprised and troubled by the story about adjuncts that Murphy purports to tell. Murphy's argument, that a teaching substructure in rhetoric and composition exists but remains invisible and that to recognize such a substructure would cost universities "very little," is based on a notion of universities that has not existed in most places for a very long time, if ever. Murphy's idea of a "teaching track" that supports full-time faculty research is based on a conception of a university at a handful of research institutions. Schools that train graduate students, produce the bulk of scholarship in the field of rhetoric and composition, and grant PhDs are about 7 percent of the total number of universities in this country (Phelan 76). To make an argument about adjunct work and adjunct labor without considering the other 93 percent of us seems to us to be thoughtless, at best, and unethical, at worst. The other aspect of adjuncting that Murphy leaves out is, of course, gender. As Theresa Enos writes, "When a field has been feminized and when a disproportionate number of its workers are female, that field is devalued and is subject to both disciplinary and gender bias" (43). As the latest report on Women in the Profession indicates, women are still "more likely than white men ... to obtain jobs in lower-paying institutions .., and they tend to linger
- Published
- 2001
15. 'But You Aren't White:' Racial Perceptions and Service-Learning
- Author
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Ann E. Green, Ann E. Green, Ann E. Green, and Ann E. Green
- Abstract
Michigan Journal of Community Service Learning: vol. 8, no. 1, (dlps) 3239521.0008.102, http://hdl.handle.net/2027/spo.3239521.0008.102, This work is protected by copyright and may be linked to without seeking permission. Permission must be received for subsequent distribution in print or electronically. Please contact mpub-help@umich.edu for more information.
16. Drug metabolism interactions with anticancer agents in mice
- Author
-
Andreas Gescher and Ann E. Green
- Subjects
Male ,Dacarbazine ,Antineoplastic Agents ,Mice, Inbred Strains ,Pharmacology ,Biology ,Procarbazine ,Biochemistry ,Mixed Function Oxygenases ,Mice ,chemistry.chemical_compound ,In vivo ,Chlorozotocin ,medicine ,Animals ,Drug Interactions ,Nitroanisole O-Demethylase ,Carmustine ,Body Weight ,Organ Size ,Lomustine ,Kinetics ,Liver ,Pharmaceutical Preparations ,chemistry ,Uncompetitive inhibitor ,Drug metabolism ,medicine.drug - Abstract
The effects of seven antineoplastic agents (dacarbazine, procarbazine. hexamethylmelamine. carmustine, lomustine, chlorozotocin, p -carbmethoxyphenyldimethyltriazene) on oxidative drug metabolism were studied. With the exception of dacarbazine and chlorozotocin they inhibited p -nitroanisol O -demethylase in vitro in liver 9000 g supernatant of CBALac mice. The inhibition mode was mixed for procarbazine and hexamethylmelamine, apparent K i values for these drugs were 1.9 and O.8mM. Carmustine and p -carbmethoxyphenyldimethyltriazene (CMPDT) showed uncompetitive inhibition. Procarbazine inhibition is mediated by a metabonate and the inhibition by CMPDT is reduced by non-specific binding. The administration of these agents at tumour curative doses in vivo lead only to weak to negligible depression of O - and N -demethylase activities 10 min, l day and 3 days after cessation of drug administration.
- Published
- 1980
17. ESRRA-C11orf20 is a recurrent gene fusion in serous ovarian carcinoma.
- Author
-
Julia Salzman, Robert J Marinelli, Peter L Wang, Ann E Green, Julie S Nielsen, Brad H Nelson, Charles W Drescher, and Patrick O Brown
- Subjects
Biology (General) ,QH301-705.5 - Abstract
Every year, ovarian cancer kills approximately 14,000 women in the United States and more than 140,000 women worldwide. Most of these deaths are caused by tumors of the serous histological type, which is rarely diagnosed before it has disseminated. By deep paired-end sequencing of mRNA from serous ovarian cancers, followed by deep sequencing of the corresponding genomic region, we identified a recurrent fusion transcript. The fusion transcript joins the 5' exons of ESRRA, encoding a ligand-independent member of the nuclear-hormone receptor superfamily, to the 3' exons of C11orf20, a conserved but uncharacterized gene located immediately upstream of ESRRA in the reference genome. To estimate the prevalence of the fusion, we tested 67 cases of serous ovarian cancer by RT-PCR and sequencing and confirmed its presence in 10 of these. Targeted resequencing of the corresponding genomic region from two fusion-positive tumor samples identified a nearly clonal chromosomal rearrangement positioning ESRRA upstream of C11orf20 in one tumor, and evidence of local copy number variation in the ESRRA locus in the second tumor. We hypothesize that the recurrent novel fusion transcript may play a role in pathogenesis of a substantial fraction of serous ovarian cancers and could provide a molecular marker for detection of the cancer. Gene fusions involving adjacent or nearby genes can readily escape detection but may play important roles in the development and progression of cancer.
- Published
- 2011
- Full Text
- View/download PDF
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