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Background: To evaluate the dissociation effect of optical fluorescence platelet counting of BC-6800 hematology analyzer on ethylene diamine tetraacetic acid-dependent pseudo thrombocytopenia (EDTA- PTCP) samples. Methods: Twenty-three finally identified EDTA-PTCP samples were recruited in this study using criteria as follow: (I) impedance platelet counts lower than 100×10 9 /L with instrument “platelet aggregation” flag; (II) existence of platelet clumps in the blood smear; (III) obviously higher platelet counts without “platelet aggregation” flag and no platelet clumps in blood smear after repeating phlebotomy using citrate anticoagulated tubes. The BC-6800 hematology analyzer and the XE-2100 hematology analyzer were used to test 23 EDTA-PTCP samples and 30 controls on both the impedance channel and the reticulocyte channel. The dissociation rate was defined as optical fluorescence platelet counts in the EDTA tubes/impedance platelet counts in citrate tubes ×100%. Results: BC-6800 analyzer’s optical fluorescence platelet counts of EDTA-PTCP samples were significantly higher than impedance platelet counts (t=4.33, P=0.00) and comparable with the platelet counts of re-collected samples in tubes containing citrate anticoagulant. On BC-6800 hematology analyzer, 22 of 23 EDTA-PTCP samples showed a dissociation rate greater than 80%, and the average dissociation rate was 93%. On the XE-2100 hematology analyzer, 1 of the 17 EDTA-PTCP samples showed a dissociation rate greater than 80%, and the average dissociation rate was 56%. Conclusions: Optical fluorescence platelet counting of BC-6800 Hematology Analyzer is effective for the correction of spurious low platelet counts in EDTA-PTCP patients, and its dissociation effect on EDTA- PTCP samples was independent of fluorescent dye staining.

Clinical studies have shown that oral vitamin C supplementation can reduce serum uric acid levels in multiple populations and may also improve acute mountain sickness. However, it is unclear whether this protocol can improve high-altitude hyperuricemia. Therefore, we aimed to evaluate the role of vitamin C supplementation on high-altitude hyperuricemia.A preliminary prospective control study was performed in 2015. Young male army recruits (n = 66), who had recently arrived on the Tibetan Plateau for the first time, were recruited for study I. Subjects were assigned to either the vitamin C group, who took an oral daily dose of 500 mg vitamin C for 1 month, or the blank control group, who had no intervention. The levels of serum uric acid, serum creatinine, and blood urea nitrogen were monitored at baseline and at the end of 1 month. In a second study II in 2016 (n = 120), the effect of 500 mg/d vitamin C on high-altitude hyperuricemia was compared with 75 IU/d of vitamin E.In study I, the level of serum uric acid at 1 month was significantly higher than at baseline (436.1 ± 79.3 μmol/L vs. 358.0 ± 79.8 μmol/L, p 0.001) and the prevalence of hyperuricemia was also significantly higher (63.6% [95% confidence interval, CI: 52.0%-75.2%] vs. 19.7% [95% CI: 10.1%-29.3%], p 0.001). Both the level of serum uric acid (411.5 ± 74.2 μmol/L vs. 460.8 ± 54.8 μmol/L, p = 0.003) and the prevalence of hyperuricemia (48.5% [95% CI: 31.4%-65.6%] vs. 78.8% [95% CI: 64.9%-92.7%], p = 0.020) were significantly lower in the vitamin C group than in the blank control group. In study II, the levels of serum uric acid and the frequency of hyperuricemia also increased over 1 month and were similar in the vitamin C and the vitamin E groups at both baseline and 1 month (p 0.05). The change in serum uric acid was positively correlated with both the changes in serum creatinine (r = 0.599, p 0.001) and blood urea nitrogen (r = 0.207, p = 0.005).These findings indicate that healthy young men develop an increase in serum uric acid within a month of moving from low to high altitude. Oral vitamin C supplementation can safely reduce this increase at a low cost.

We report here one-pot green synthetic method for preparing popcorn-like Au@Polyluminol nanoflowers by using the HAuCl4 to directly oxidize luminol at a specific controlled mass ratio of HAuCl4: luminol in hydrothermal conditions for the first time. The Au@Polyluminol nanoflowers were attached to the surface of a GCE electrode to form a highly solid-state electrochemiluminescent (ECL) sensor, which exhibited strong and stable ECL intensity, showed excellent ECL behavior. Further, the as-prepared Au@Polyluminol nanoflowers could also be used for the immobilization of uricase on an electrode surface to fabricate a sensitive solid-state Uric Acid ECL biosensor, which indicate that the Au@Polyluminol nanoflowers is a promising candidate for constructing various sensitive solid-state ECL sensors and promoting the application in electroanalytical and biochemical analysis.

SDS-PAGE was applied to determine trypsin activity and inhibition. After the hydrolysis by trypsin to substrate bovine serum albulnin (BSA) under different temperatures and pH, the hydrolysis degree of BSA was conducted using SDS-PAGE. From the quantitative analysis to the electrophoresis bands of BSA and its hydrolysis products in SDS-PAGE pattern, the change of trypsin activity was determined, and then the optimum temperature at 40°C and the optimum pH at pH 8.5 - 8.7 for trypsin activity were obtained. All the target bonds in BSA molecule could be hydrolyzed at the same time by trypsin. The inhibition was due to the binding of inhibitor to trypsin, which made it impossible for trypsin to touch the substrate protein. SDS-PAGE was demonstrated to be also an effect method for assaying the characteristics of trypsin activity and its inhibition.

Emotion is the mental activity brought by peoples' physiology needs. Feeling is the mental activity brought by human social needs. Emotion is the outward expression of feeling, while feeling is the essence of emotion. The two are like the double helix structure of DNA that go together and are inseparable. ② The music work created by the composer stimulates people's senses first to stimulate the physiological changes. Therefore, to produce an emotional reaction in people, bring emotional experience and achieve the aim of experiencing the affections expressed through the music. ③ Feelings have a social source, therefore, the basis of music works and the creative standing point of the composer must root in the social reality. Only in this way can the music works have a richer expression of affections.

Over the past few years, with rapidly growing popularity of the Internet and the world wide web, the performance of microprocessors and capacity of switching model has been growing exponentially and this growth can be expected to continue in the foreseeable future.[1]