33 results on '"Anisakid nematode"'
Search Results
2. The Mediterranean Mussel (Mytilus galloprovincialis) as Intermediate Host for the Anisakid Sulcascaris sulcata (Nematoda), a Pathogen Parasite of the Mediterranean Loggerhead Turtle (Caretta caretta)
- Author
-
Mario Santoro, Erica Marchiori, Marialetizia Palomba, Barbara Degli Uberti, Federica Marcer, and Simonetta Mattiucci
- Subjects
anisakid nematode ,mussel parasite ,mytilus galloprovincialis ,sulcascaris sulcata ,tyrrhenian sea ,Medicine - Abstract
Sulcascaris sulcata (Anisakidae), a pathogenic nematode of sea turtles, may cause ulcerous gastritis with different degrees of severity. Previous studies demonstrated a high prevalence of infection in the Mediterranean loggerhead turtle (Caretta caretta), although no data on the potential intermediate hosts of this nematode has been published thus far from the Mediterranean basin. Here, using molecular analyses, we demonstrated that the cross sections of nematode larvae observed histologically in Mediterranean mussels (Mytilus galloprovincialis) collected from a farm along the Tyrrhenian coast of southern Italy belong to S. sulcata. The BLAST analysis of sequences at the ITS2 region of rDNA and mtDNA cox2 gene loci here obtained from samples of two Mediterranean mussels containing nematode larvae showed 100% homology with those at the same gene loci from the adults of S. sulcata collected from the Mediterranean Sea and deposited in GenBank. To our knowledge, this study is the first to present data on a potential intermediate host of S. sulcata in the Mediterranean basin and to report a nematode parasite from the Mediterranean mussel.
- Published
- 2020
- Full Text
- View/download PDF
3. Anisakid Nematodes and Anisakiasis
- Author
-
Lymbery, A.J., Cheah, F.Y., Black, Samuel J., editor, Seed, J. Richard, editor, Murrell, K. Darwin, editor, and Fried, Bernard, editor
- Published
- 2007
- Full Text
- View/download PDF
4. Parasitic Anisakid Nematode Isolated from Stranded Fraser’s Dolphin (Lagenodelphis hosei Fraser, 1956) from Central Philippine Waters
- Author
-
Karl Marx A. Quiazon, Tomoyoshi Yoshinaga, and Mudjekeewis D. Santos
- Subjects
0303 health sciences ,03 medical and health sciences ,0302 clinical medicine ,Anisakid nematode ,Lagenodelphis hosei ,030231 tropical medicine ,Zoology ,Biology ,biology.organism_classification ,030308 mycology & parasitology - Abstract
Cetaceans, including dolphins, serve as definitive hosts of zoonotic anisakid nematodes, which are important etiological agents for human anisakiasis and allergy-associated health risks. With limited knowledge of these zoonotic parasites from the marine environment in the Philippine waters, the stranding of a Fraser’s dolphin (Lagenodelphis hosei Fraser, 1956) off the central Philippines made it possible to identify the worm species isolated from its gut. Parasitological examinations were carried out using morphological and molecular tools. Morphologically, the SEM and LM data revealed that the specimens belong to the genus Anisakis of the Type 1 group. Molecularly, PCR-RFLP results of the ITS region generated only a single fragment pattern on all worm samples corresponding to the reported molecular keys for A. typica. Further sequence and phylogenetic analyses of both ITS rDNA and mtDNA COX2 genes confirmed the anisakid nematodes’ identity as A. typica. The molecular data obtained in this study support previous findings on the possible existence of local variants of A. typica in this region.
- Published
- 2020
- Full Text
- View/download PDF
5. Pathology and Differential Diagnoses of Internationalized Zoonoses: Summarizing Discussion of the Organizers
- Author
-
Ishikura, Hajime, Aikawa, Masamichi, Itakura, Hideyo, Ishikura, Hajime, editor, Aikawa, Masamichi, editor, Itakura, Hideyo, editor, and Kikuchi, Kokichi, editor
- Published
- 1998
- Full Text
- View/download PDF
6. Prevalence of Larval Anisakid Nematodes in Fresh Fish from Coastal Waters of Hokkaido
- Author
-
Miyamoto, K., Ishikura, Hajime, editor, and Kikuchi, Kokichi, editor
- Published
- 1990
- Full Text
- View/download PDF
7. First Record of Contracaecum rudolphii Hartwich, 1964 in Carassius gibelio (Bloch, 1782) From Turkey
- Author
-
Mala Stavrescu-Bedivan, Ozlem Ozmen, Deniz Innal, and Mehmet Oğuz Öztürk
- Subjects
Science ,Fauna ,Zoology ,Ocean Engineering ,Aquatic Science ,medicine.disease_cause ,lcsh:Aquaculture. Fisheries. Angling ,anisakidae ,Infestation ,medicine ,Parasite hosting ,nematoda ,lcsh:QH301-705.5 ,Water Science and Technology ,lcsh:SH1-691 ,Prussian carp ,Fen ,biology ,biology.organism_classification ,Anisakid nematode ,lcsh:Biology (General) ,Nematoda,Anisakidae,first record,histopathology ,Contracaecum rudolphii ,Nematode larvae ,histopathology ,Carassius ,first record - Abstract
Thirty-eight individuals of Prussian carp, Carassius gibelio (Bloch, 1782) were collected from Karataş Lake, Burdur-Turkey and analyzed for parasite fauna. We found the nematode larvae of Contracaecum rudolphii Hartwich, 1964 in one sample (prevalence 2.63%, mean intensity of infestation 27 parasites per fish). The individual parasite was found around the pancreas, fibrous connective tissue and its mesentery. To our knowledge, this is the first record of anisakid nematode, Contracaecum rudolphii in Turkey. Therefore, a new locality has been added to the geographical distribution of the parasite species. Furthermore, slight to severe inflammatory cells were seen on the infected tissue. Granulomatous reaction characterized by mononuclear cells and fibrous tissue proliferations were also seen around the parasite located areas.
- Published
- 2019
- Full Text
- View/download PDF
8. IDENTIFICATION OF ANISAKID NEMATODE L3 LARVAE INFECTION ON SKIPJACK TUNA (KATSUWONUS PELAMIS L.) FROM KUPANG WATERS, EAST NUSA TENGGARA OF INDONESIA
- Author
-
E. Suprayitno, A.M. Hariati, M. Amin, A. Aulanni’am, and L. Haryadi
- Subjects
Skipjack tuna ,Larva ,biology ,dolphin ,Whale ,fungi ,General Medicine ,Cetacean ,whale ,biology.organism_classification ,lcsh:S1-972 ,Fishery ,savu ,Anisakid nematode ,biology.animal ,Identification (biology) ,molecular ,lcsh:Agriculture (General) - Abstract
The larvae of Anisakis were living parasites and made marine mammals, birds and reptiles the definitive host. Identification of Anisakis larvae using morphological observation methods will be difficult, especially if there are only a few samples that can be identified. PCR is an identification method using DNA from a small sample quantity and can provide DNA sequence samples. This study aimed to determine the type and infection level of Anisakis sp. at skipjack tuna (Katsuwonus pelamis) from the Oeba Fish Auction (TPI) Kupang City morphologically and molecularly. Morphological analysis results of 30 Anisakis larvae showed the body parts of Anisakis larvae, namely the head, digestive tract, and tail. The infection of Anisakis nematodes in skipjack tuna found five individual nematodes in muscle tissue, 59 individuals in stomach tissue, and 1991 individuals in internal organs. Alignment results between isolates At1 and At2 against isolates A. typica comparing (outgroup), isolate At1 and At2 have high homologs. Based on the results of the study concluded that the type I Anisakis isolated from skipjack tuna (Savu Sea) was Anisakis typica.
- Published
- 2019
- Full Text
- View/download PDF
9. Intrinsic properties of anisakid nematode larvae as a potential tool for the detection in fish products
- Author
-
Horst Karl, Regina Klapper, and Ute Ostermeyer
- Subjects
Identification methods ,Biology ,Anisakiasis ,Microbiology ,Fluorescence ,Host-Parasite Interactions ,03 medical and health sciences ,Human health ,Fish Products ,Animals ,Humans ,Food-Processing Industry ,Fish processing ,030304 developmental biology ,0303 health sciences ,030306 microbiology ,Consumer health ,General Medicine ,Fish products ,Pseudoterranova decipiens ,Anisakis ,Anisakid nematode ,Larva ,Food Microbiology ,Identification (biology) ,Biochemical engineering ,Food Science - Abstract
Anisakid nematode larvae (NL) in fish products comprise a risk to human health and, if visible, lead to the rejection of these products by consumers. Therefore, great efforts are being made for the identification of these anisakid larvae to estimate the potential consumer health risk as well as to develop effective detection methods in order to prevent the introduction of heavily infected fish products into the market. The tasks of national reference laboratories include the improvement of detection methods and to promote their further development. As a prerequisite for improved detection, it is important to understand the structural properties of anisakid NL and compounds produced during host-parasite interactions. This review provides an overview of the intrinsic properties of anisakid NL and reports the latest detection methods in published literature. First, in order to define the potentially interesting intrinsic properties of anisakid nematodes for their detection, anatomy and compounds involved in host-parasite interactions are summarised. These can be used for various detection approaches, such as in the medical field or for allergen detection in fish products. In addition, fluorescence characteristics and their use as both established and promising candidates for detection methods, especially in the field of optical sensing technologies, are presented. Finally, different detection and identification methods applied by the fish processing industries and by control laboratories are listed. The review intends to highlight trends and provide suggestions for the development of improved detection and identification methods of anisakid NL in fish products.
- Published
- 2020
10. The Mediterranean Mussel (Mytilus galloprovincialis) as Intermediate Host for the Anisakid Sulcascaris sulcata (Nematoda), a Pathogen Parasite of the Mediterranean Loggerhead Turtle (Caretta caretta)
- Author
-
Simonetta Mattiucci, Federica Marcer, Mario Santoro, Marialetizia Palomba, Barbara Degli Uberti, and Erica Marchiori
- Subjects
Microbiology (medical) ,Mediterranean climate ,Mediterranean mussel ,animal structures ,Zoology ,lcsh:Medicine ,mytilus galloprovincialis ,Biology ,Mediterranean Basin ,anisakid nematode ,Mediterranean sea ,mussel parasite ,sulcascaris sulcata ,tyrrhenian sea ,Immunology and Allergy ,Molecular Biology ,General Immunology and Microbiology ,fungi ,lcsh:R ,Intermediate host ,biology.organism_classification ,Mytilus ,Anisakidae ,Infectious Diseases ,Nematode - Abstract
Sulcascaris sulcata (Anisakidae), a pathogenic nematode of sea turtles, may cause ulcerous gastritis with different degrees of severity. Previous studies demonstrated a high prevalence of infection in the Mediterranean loggerhead turtle (Caretta caretta), although no data on the potential intermediate hosts of this nematode has been published thus far from the Mediterranean basin. Here, using molecular analyses, we demonstrated that the cross sections of nematode larvae observed histologically in Mediterranean mussels (Mytilus galloprovincialis) collected from a farm along the Tyrrhenian coast of southern Italy belong to S. sulcata. The BLAST analysis of sequences at the ITS2 region of rDNA and mtDNA cox2 gene loci here obtained from samples of two Mediterranean mussels containing nematode larvae showed 100% homology with those at the same gene loci from the adults of S. sulcata collected from the Mediterranean Sea and deposited in GenBank. To our knowledge, this study is the first to present data on a potential intermediate host of S. sulcata in the Mediterranean basin and to report a nematode parasite from the Mediterranean mussel.
- Published
- 2020
11. The Mediterranean Mussel (
- Author
-
Mario, Santoro, Erica, Marchiori, Marialetizia, Palomba, Barbara Degli, Uberti, Federica, Marcer, and Simonetta, Mattiucci
- Subjects
Tyrrhenian Sea ,animal structures ,mussel parasite ,Mytilus galloprovincialis ,Sulcascaris sulcata ,fungi ,anisakid nematode ,Article - Abstract
Sulcascaris sulcata (Anisakidae), a pathogenic nematode of sea turtles, may cause ulcerous gastritis with different degrees of severity. Previous studies demonstrated a high prevalence of infection in the Mediterranean loggerhead turtle (Caretta caretta), although no data on the potential intermediate hosts of this nematode has been published thus far from the Mediterranean basin. Here, using molecular analyses, we demonstrated that the cross sections of nematode larvae observed histologically in Mediterranean mussels (Mytilus galloprovincialis) collected from a farm along the Tyrrhenian coast of southern Italy belong to S. sulcata. The BLAST analysis of sequences at the ITS2 region of rDNA and mtDNA cox2 gene loci here obtained from samples of two Mediterranean mussels containing nematode larvae showed 100% homology with those at the same gene loci from the adults of S. sulcata collected from the Mediterranean Sea and deposited in GenBank. To our knowledge, this study is the first to present data on a potential intermediate host of S. sulcata in the Mediterranean basin and to report a nematode parasite from the Mediterranean mussel.
- Published
- 2020
12. Anisakid infection levels in fresh and canned cod liver: Significant reduction through liver surface layer removal
- Author
-
Kurt Buchmann, Miguel González-Muñoz, Horst Karl, Noelia Carballeda-Sangiao, Regina Klapper, Thomas Kuhn, and Hannah Malene Jensen
- Subjects
0301 basic medicine ,fungi ,Anisakis simplex ,food and beverages ,030108 mycology & parasitology ,Biology ,Contamination ,biology.organism_classification ,Pseudoterranova decipiens ,03 medical and health sciences ,030104 developmental biology ,Anisakid nematode ,Nematode ,Baltic sea ,Nematode larvae ,Gadus ,Food science ,Food Science ,Biotechnology - Abstract
Canned liver is a highly valuable and healthy product from the important commercial fish species cod Gadus morhua. Due to high levels of contaminants such as dioxins and dioxin-like PCBs (polychlorinated biphenyls) in cod liver products from the Baltic Sea, the grounds for supply of cod liver were relocated to areas around Iceland in the Irminger Sea with lower levels of contaminants. In the present study, 62 canned samples, as well as 132 fresh cod livers from Greenland and Iceland, were analysed in order to assess the infection levels with anisakid nematode species. High prevalence and abundance of the potentially zoonotic nematode species Anisakis simplex s.s. and Pseudoterranova decipiens s.s. were found in the fresh livers. Lower infection levels in canned livers compared to fresh livers might indicate successful industrial processing measures in order to reduce nematode larvae in the final products. However, two heat stable allergens, Ani s 1 and Ani s 4, were verified from isolated nematode larvae from canned products. The effectiveness of methods to further reduce the number of nematode larvae during industrial processing of cans with cod liver is discussed.
- Published
- 2018
- Full Text
- View/download PDF
13. Identification of anisakid nematodes with zoonotic potential from Europe and China by single-strand conformation polymorphism analysis of nuclear ribosomal DNA.
- Author
-
Zhu, X. Q., Podolska, M., Liu, J. S., Yu, H. Q., Chen, H. H., Lin, Z. X., Luo, C. B., Song, H. Q., and Lin, R. Q.
- Published
- 2007
- Full Text
- View/download PDF
14. Inventorying biodiversity of anisakid nematodes from the Austral Region: a hotspot of genetic diversity?
- Author
-
Paolo Cipriani, Michela Paoletti, Stephen C. Webb, Giuseppe Nascetti, Simonetta Mattiucci, Juan T. Timi, Klimpel, Sven, Kuhn, Thomas, and Melhorn, Heinz
- Subjects
0301 basic medicine ,Biogeography ,030231 tropical medicine ,Biodiversity ,Biology ,Spatial distribution ,ANISAKIDAE ,Ciencias Biológicas ,03 medical and health sciences ,0302 clinical medicine ,host-parasite association ,Hotspot (geology) ,marine trophic webs ,Marine ecosystem ,anisakid nematodes ,austral region ,genetic diversity ,SOUTHERN OCEAN ,Genetic diversity ,Ecology ,Ecología ,030108 mycology & parasitology ,Anisakid nematode ,Taxon ,BIODIVERSITY ,CIENCIAS NATURALES Y EXACTAS - Abstract
Inventorying of anisakid nematode biodiversity is the discovering, surveying, quantifying and mapping of species, populations and their genetic diversity and variability. This aim, however, is compromised if discrimination of anisakid taxa relies solely on morphological features. Therefore, the accurate detection and delimitation of cryptic anisakid species requires molecular-based assessments. This, in turn, permits elucidation of patterns and process in their evolution and ecology, including biogeography, host-parasite association and co-evolution. In addition, a true picture of anisakids and their genetic diversity facilitates understanding of their temporal and spatial distribution also related to their hosts demographic changes and marine ecosystem food webs. This review represents an inventorying of the biodiversity, at species and gene level, of those anisakid species so far discovered belonging to the genera Anisakis, Pseudoterranova and Contracaecum, from the Austral Region, including: (1) taxa recognized as “biological species” based on the application of different molecular genetic markers; (2) current molecular/genetic approaches to identify them at any life-history stage; (3) ecological data relating to the geographical distribution, definitive host-association and host-preferences; (4) estimates of genetic variability values inferred from nuclear and mitochondrial genes, as a possible indicator of the integrity of marine food webs; (5) data so far known concerning the possible zoonotic role of those anisakid species to humans. Fil: Mattiucci, Simonetta. Università degli studi di Roma "La Sapienza"; Italia Fil: Paoletti, Michela. Universita Di Tuscia; Italia Fil: Cipriani, Paolo. Università degli studi di Roma "La Sapienza"; Italia. Universita Di Tuscia; Italia Fil: Webb, Stephen C.. Cawthron Institute; Nueva Zelanda Fil: Timi, Juan Tomas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina Fil: Nascetti, Giuseppe. Universita Di Tuscia; Italia
- Published
- 2017
15. Anisakid Parasites in Commercial Hake Ceviche in Southern Chile
- Author
-
Patricio Torres and Pablo Torres-Frenzel
- Subjects
Veterinary medicine ,Larva ,Muscles ,fungi ,Food Contamination ,Biology ,Microbiology ,Anisakis ,Fishery ,Gadiformes ,Anisakid nematode ,Seafood ,Hake ,Southern hake ,Merluccius australis ,Animals ,%22">Fish ,Chile ,Food Science - Abstract
The objective of this research was to determine the occurrence of anisakid nematode larvae in hake ceviche sold in restaurants in Valdivia (39°48'S, 73°14'W) and Niebla (39°49'S, 73°22'W), Chile. Between August and November 2012, 78 portions of ceviche were collected (6 from each of the 13 restaurants that sell this product). Each portion was weighed and divided into approximately 30-g samples, which were placed in petri dishes with 0.15 M NaCl. All samples were manually shredded and then examined with a stereomicroscope. Muscles of 41 southern hake (Merluccius australis), a fish sold fresh in Valdivia, also were examined by candling to determine the presence of anisakid larvae. The presence of Pseudoterranova larvae in ceviche sold in Chile was identified for the first time. The pH of ceviche ranges from 4.1 to 4.8, which favors the presence of viable anisakid larvae that tolerate the acid pH similar to that found in the stomach of their mammalian host. No significant differences (P0.05) in the prevalence, mean abundance, and mean density of anisakid larvae in ceviche were found between localities. Larvae were detected in ceviche from 3 of 6 restaurants in Valdivia and 4 of 7 restaurants in Niebla. Of the 78 examined portions of ceviche, 21.8% had larvae. The prevalence of viable larvae was 16.7 and 7.1% in the examined portions from Valdivia and Niebla, respectively. In the 41 hake muscle samples from Valdivia, the prevalence (4.9%), mean abundance (0.1), and mean density (0.03) was the same for Pseudoterranova and Anisakis larvae. No inspection processes or pretreatments are currently in place for raw fish to achieve safe conditions for ceviche in restaurants from Valdivia and Niebla.
- Published
- 2014
- Full Text
- View/download PDF
16. The Mediterranean Mussel (Mytilus galloprovincialis) as Intermediate Host for the Anisakid Sulcascaris sulcata (Nematoda), a Pathogen Parasite of the Mediterranean Loggerhead Turtle (Caretta caretta).
- Author
-
Santoro, Mario, Marchiori, Erica, Palomba, Marialetizia, Degli Uberti, Barbara, Marcer, Federica, and Mattiucci, Simonetta
- Subjects
LOGGERHEAD turtle ,MYTILUS galloprovincialis ,NEMATODES ,MUSSELS ,TREMATODA ,PARASITES ,SEA turtles ,MITOCHONDRIAL DNA - Abstract
Sulcascaris sulcata (Anisakidae), a pathogenic nematode of sea turtles, may cause ulcerous gastritis with different degrees of severity. Previous studies demonstrated a high prevalence of infection in the Mediterranean loggerhead turtle (Caretta caretta), although no data on the potential intermediate hosts of this nematode has been published thus far from the Mediterranean basin. Here, using molecular analyses, we demonstrated that the cross sections of nematode larvae observed histologically in Mediterranean mussels (Mytilus galloprovincialis) collected from a farm along the Tyrrhenian coast of southern Italy belong to S. sulcata. The BLAST analysis of sequences at the ITS2 region of rDNA and mtDNA cox2 gene loci here obtained from samples of two Mediterranean mussels containing nematode larvae showed 100% homology with those at the same gene loci from the adults of S. sulcata collected from the Mediterranean Sea and deposited in GenBank. To our knowledge, this study is the first to present data on a potential intermediate host of S. sulcata in the Mediterranean basin and to report a nematode parasite from the Mediterranean mussel. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
17. Anisakiasis
- Author
-
Bier, Jeffrey W., Balows, A., editor, Hausler, W. J., Jr., editor, Ohashi, M., editor, Turano, A., editor, and Lennete, E. H., editor
- Published
- 1988
- Full Text
- View/download PDF
18. Current status of anisakid nematode larvae infection in marine fishes caught from the coastal area of Korea between 2010 and 2012
- Author
-
Chan-Hyeok Jeon, Wi-Sik Kim, Do-Hyung Kim, Myung-Joo Oh, and Jeong-Ho Kim
- Subjects
Fishery ,Larva ,Veterinary medicine ,Anisakid nematode ,biology ,Raphidascaris lophii ,Internal transcribed spacer ,biology.organism_classification ,Anisakis ,Ribosomal DNA ,Molecular identification ,Anisakis pegreffii - Abstract
** A survey was conducted to investigate infection of anisakid nematode larvae in 243 wild marine fishes caught from the southern coastal area of Korea between 2010 and 2012. The samples comprised fishes from 9 orders, 30 families and 50 species. Total infection rate of anisakid nematode larvae was 10.7% (26/243 fish), which comprised from Yeosu, 7.4% (7/95) in 2010 and 22.7% (5/22) in 2011; from Jeju, 8.2% (5/61) in 2011; from Wando, 40.9% (9/22) in 2012. Anisakid nematode larvae were not detected in Tongyoung and Wando samples in 2011. Molecular identification of the 89 worms from 26 fish was conducted by PCR-RFLP and/or sequence analysis of internal transcribed spacer (ITS) region of ribosomal DNA. From the results, 6 kinds of anisakis species were identified: Anisakis pegreffii (infection rate: 53.9%, 48/89 worms), Hysterothylacium aduncum (38.2%, 34/89), H. fabri (3.4%, 3/89), hybird (A. simplex X A. pegreffii) (2.4%, 2/89), A. simplex (1.1%, 1/89) and Raphidascaris lophii (1.1%, 1/89). The rate of single infection was 80.8% (21/26 infected fish), while 19.2% (5/26) showed mixed infection with 2 to 3 different anisakis species.
- Published
- 2012
- Full Text
- View/download PDF
19. Viability Test Device for anisakid nematodes
- Author
-
Horst Karl, Michael Kroeger, Bernhard Simmler, and Peter Singer
- Subjects
0301 basic medicine ,animal structures ,genetic structures ,030106 microbiology ,Biophysics ,Anisakis ,Article ,Uncertainty factor ,03 medical and health sciences ,parasitic diseases ,14. Life underwater ,lcsh:Social sciences (General) ,lcsh:Science (General) ,Multidisciplinary ,biology ,Inner pressure ,Wavelength range ,fungi ,biology.organism_classification ,030104 developmental biology ,Anisakid nematode ,Nematode larvae ,lcsh:H1-99 ,Statistical error ,Visual observation ,Biological system ,lcsh:Q1-390 - Abstract
Up to now the visual inspection of mobility of isolated anisakid larvae serves as a measure of viability and possible risk of infection. This paper presents a new method to rule out unreliability – caused by the temporary immobility of the larvae and by the human uncertainty factor of visual observation. By means of a Near infrared (NIR) imaging method, elastic curvature energies and geometric shape parameters were determined from contours, and used as a measure of viability. It was based on the modelling of larvae as a cylindrical membrane system. The interaction between curvatures, contraction of the longitudinal muscles, and inner pressure enabled the derivation of viability from stationary form data. From series of spectrally signed images within a narrow wavelength range, curvature data of the larvae were determined. Possible mobility of larvae was taken into account in statistical error variables. Experiments on individual living larvae, long-term observations of Anisakis larvae, and comparative studies of the staining method and the VTD measurements of larvae from the tissue of products confirmed the effectiveness of this method. The VTD differentiated clearly between live and dead nematode larvae isolated from marinated, deep-frozen and salted products. The VTD has been proven as excellent method to detect living anisakid nematode larvae in fishery products and is seen as useful tool for fish processing industry and control authorities.
- Published
- 2018
- Full Text
- View/download PDF
20. Third-Stage Larvae of Anisakis simplex (Rudolphi, 1809) in the Red Sea Fishes, Yemen Coast
- Author
-
Ali B. Al-Zubaidy
- Subjects
Fish market ,Third stage larvae ,Anisakis simplex ,Aquatic Science ,Biology ,Oceanography ,Fishery ,Anisakid nematode ,Distribution pattern ,Nematode larvae ,Environmental Chemistry ,Helminths ,%22">Fish ,Water Science and Technology - Abstract
There is much less information on the occurrence of parasitic nematodes in the Red Sea fish, Yemeni Coast, therefore, the aim of this study was to assess the anisakid nematode distribution pattern in the fish collected from fish market (Al-Mehwat) in the Hodeidah City, during the period between January 2006 - March 2007.
- Published
- 2010
- Full Text
- View/download PDF
21. Human Infections with Pseudoterranova cattani Nematodes, Chile
- Author
-
Hiromu Sugiyama, Cristian Ramirez, Thomas Weitzel, Reinaldo Rosas, Rubén Mercado, and Hiroshi Yamasaki
- Subjects
Pseudoterranovosis Anisakid nematode ,Microbiology (medical) ,Adult ,Male ,Subfamily ,Letter ,Epidemiology ,Linguatula serrata ,lcsh:Medicine ,Zoology ,Biology ,parasites ,Anisakis ,lcsh:Infectious and parasitic diseases ,Pseudoterranovosis ,Fish Diseases ,Sensu ,Paratenic ,Ascaridoidea ,Fish Products ,Parasite hosting ,Animals ,Humans ,lcsh:RC109-216 ,Chile ,Letters to the Editor ,Pseudoterranova cattani ,fish ,lcsh:R ,Anisakis simplex ,foodborne illness ,Anatomy ,Middle Aged ,Human Infections with Pseudoterranova cattani Nematodes, Chile ,human infections ,biology.organism_classification ,Pseudoterranova decipiens ,Parasitic diseases ,zoonoses ,Ascaridida Infections ,Anisakid nematode ,Infectious Diseases ,Larva ,Female - Abstract
To the Editor: Anisakidosis is an emerging foodborne zoonosis caused by nematode larvae of the Anisakinae subfamily, which includes the genera Anisakis, Pseudoterranova, and Contracecum (1). In natural cycles, anisakid larvae are transmitted to marine mammals or piscivorous birds when they eat raw saltwater fish or squid. In the human incidental host, larvae attach to the mucosa of the gastrointestinal tract, causing clinical features ranging from asymptomatic carriage to severe abdominal pain with complications, such as gastric perforation (2). Microscopical diagnosis is hampered by the lack of distinguishing morphologic characteristics in larval stages (1). Recently, molecular genetic techniques have shown that the main species, Anisakis simplex and Pseudoterranova decipiens, are in fact species groups with distinct geographic and biologic characteristics (3,4). The P. decipiens complex consists of at least 6 sibling species (Technical Appendix Table). We report 4 human infections with P. cattani diagnosed during 2012–2014. The case-patients were adults 22–59 years of age; 2 were female, and all lived in Santiago, Chile. Additional anamnestic and clinical data were available for 3 patients: all spontaneously regurgitated the parasites without having other gastrointestinal complaints. All 3 reported eating ceviche, a dish made of raw marine fish marinated in lemon juice. One patient reported a tingling sensation and coughs before the expulsion of a highly motile larva (Video). This patient was awaiting oral surgery after a bicycle accident and had eaten the last raw fish dish 2 weeks previously. Initially, parasites were identified by morphologic criteria. Larvae were 20 mm long, were of whitish to reddish color, and had 3 anterior lips (Technical Appendix Figure 1). Because of the presence of an anteriorly directed cecum (Technical Appendix Figure 2), they were assigned to Pseudoterranova species. Video Actively mobile larva of Pseudoterranova cattani after oral expulsion. For further molecular identification, DNA samples were extracted by using a DNeasy Blood and Tissue Kit (QIAGEN K.K., Tokyo, Japan). The rRNA gene containing 2 internal transcribed spacer (ITS) regions was amplified by PCR using primers NC5 and NC2, as previously described (5). PCR products were sequenced by using a BigDye Terminator Cycle Sequencing Kit (Applied Biosystems Inc., Foster City, CA, USA) on an automated sequencer (ABI3100, Applied Biosystems). Sequence similarities were determined by a BLAST search of DDBJ (http://blast.ddbj.nig.ac.jp/top-j.html). The GENETYX-WIN program version 7.0 (Software Development Co., Tokyo, Japan) facilitated sequence alignment and comparison. Within the 4 ITS sequences of amplicons obtained, all were 100% identical, and alignment with the other P. cattani sequence differed only in 1 nt. ITS sequences of 2 isolates are available in GenBank (accession nos. {"type":"entrez-nucleotide","attrs":{"text":"KF781284","term_id":"576106588","term_text":"KF781284"}}KF781284 and {"type":"entrez-nucleotide","attrs":{"text":"KF781285","term_id":"576106589","term_text":"KF781285"}}KF781285). All P. cattani sequences showed a previously described deletion of ≈14 bases (Table), which is not observed in other members of the P. decipiens species complex (5). Table Alignment (comparison) of nucleotide sequences of the ITS1 gene of Pseudoterranova cattani and the Chilean specimen and P. decipiens* This study identified P. cattani as a parasite capable of infecting humans. The definitive natural host of this parasite is the South American sea lion, Otaria byronia. At least 4 species of coastal fish were described as intermediate or paratenic hosts, including popular Chilean food fish species, such as Merluccius gayi, Genypterus maculatus, and Cilus gilberti (6). The spectrum of species causing human pseudoterranovosis is uncertain because most cases were reported as P. decipiens (sensu lato) or Pseudoterranova sp. Only recently, 1 case of P. azarasi infection has been documented in a patient from Japan (7). Although comparative studies are lacking, Pseudoterranova larvae seem to be less invasive and cause milder symptoms than Anisakis larvae (2,8). In the cases reported here, larvae were spontaneously expelled without further symptoms, except in 1 patient who reported the typical feature of noninvasive pseudoterranovosis, also described as “tingling throat syndrome” (8), a foreign body sensation accompanied by cough and retching. In Chile, ≈30 human cases have been reported, all diagnosed as P. decipiens or Pseudoterranova sp. by morphologic criteria (9,10). Most patients described mild oropharyngeal complaints and cough. More severe manifestations similar to parasitic pharyngitis caused by Fasciola hepatica or Linguatula serrata seem to be absent, although 1 patient had symptoms of asphyxia (9). The extent to which these cases in Chile were caused by P. cattani is uncertain because molecular diagnosis was not performed. The length of stay and location within the human gastrointestinal tract of Pseudoterranova larvae are unknown, but as indicated by 1 case in our report, lack of symptoms for up to 2 weeks is possible. These cases demonstrate that P. cattani is an incidental human parasite causing oropharygeal pseudoterranovosis. To better understand the epidemiology and clinical relevance of these emerging fishborne zoonotic infections, molecular diagnostic techniques need to be more widely applied, especially in regions where raw fish is part of the regular diet, such as in many parts of South America.
- Published
- 2015
22. Highlights of the symposium on nematode parasites held at AFSSA, Maisons-Alfort, France, the 26th of November 2004
- Author
-
Le Guerhier Franck, Boireau Pascal, Vallée Isabelle, Unité mixte de recherche biologie moléculaire et immunologie parasitaires et fongiques, Institut National de la Recherche Agronomique (INRA)-École nationale vétérinaire d'Alfort (ENVA)-Agence Française de Sécurité Sanitaire des Aliments (AFSSA)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Agence Française de Sécurité Sanitaire des Aliments (AFSSA)-École nationale vétérinaire - Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), and Institut National de la Recherche Agronomique (INRA)-École nationale vétérinaire - Alfort (ENVA)-Agence Française de Sécurité Sanitaire des Aliments (AFSSA)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)
- Subjects
Microbiology (medical) ,biology ,[SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE] ,Zoology ,Physiology ,TRICHINELLA SPECIES ,BENZIMIDAZOLE ,biology.organism_classification ,Microbiology ,Infectious Diseases ,Nematode ,Genetics ,Molecular Biology ,ComputingMilieux_MISCELLANEOUS ,Ecology, Evolution, Behavior and Systematics ,ANISAKID NEMATODE ,RELATION HOTE-PARASITE - Abstract
International audience
- Published
- 2006
- Full Text
- View/download PDF
23. Comparison of a Manual and an Automated Method to Estimate the Number of Uterine Eggs in Anisakid Nematodes: To Coulter or Not to Coulter. Is That the Question?
- Author
-
J. Antonio Raga, Juan Antonio Balbuena, Francisco E. Montero, David J. Marcogliese, and M. Victoria Herreras
- Subjects
Veterinary medicine ,Seals, Earless ,Biology ,Coulter counter ,Ascaridoidea ,Phocoena ,Animals ,Parasite Egg Count ,Ecology, Evolution, Behavior and Systematics ,Analysis of Variance ,Ecology ,Contracaecum osculatum ,Uterus ,Anisakis simplex ,Reproducibility of Results ,biology.organism_classification ,Pseudoterranova decipiens ,Anisakis ,Ascaridida Infections ,Fertility ,Nematode ,Anisakid nematode ,Vagina ,Female ,Parasitology ,Automated method - Abstract
Studies reporting numbers of eggs in vagina and utero in nematodes often give little information of the technique used for the estimations. This situation hampers comparison among studies, because, so far, differences in estimations provided by different techniques have not been assessed. This note examines whether a manual method based on visual counts in aliquots and an automated method using a Coulter counter yield equivalent estimations of egg numbers in vagina and utero of 3 anisakid nematode species (Anisakis simplex, Pseudoterranova decipiens, and Contracaecum osculatum). The number of eggs from 50 females per nematode species was estimated using both techniques. The automated and manual methods yielded similar egg counts (correlation coefficients >0.9 in the 3 species), but the methods were not always statistically equivalent. The automated method was more precise and seemed less dependent on egg density, whereas the manual method was less time-consuming (contrary to previous perceptions) and less expensive. Despite the higher precision of automated counts, the manual technique seemed to produce similar estimates; thus, it may be particularly useful in developing countries where nematode parasitism is prevalent in humans and domestic animals, but scientific resources are limited.
- Published
- 2007
- Full Text
- View/download PDF
24. Anisakid (Nematoda) Infestations in Icelandic Grey Seals (Halichoerus grypusFabr.)
- Author
-
E Hauksson and D Ólafsdóttir
- Subjects
Phocascaris cystophorae ,Ecology ,Contracaecum osculatum ,Anisakis simplex ,Zoology ,Biology ,Oceanography ,biology.organism_classification ,Pseudoterranova decipiens ,Anisakid nematode ,Nematode ,Abundance (ecology) ,Seasonal breeder - Abstract
Anisakid nematode infestations in stomachs of 196 grey seals from Icleandic waters were examined during the period 1989–93, and Pseudoterranova decipiens s . l . , Contracaecum osculatum s.l., Phocascaris cystophorae and Anisakis simplex s.l. were found in seals from all areas and seasons. No difference in nematode abundance was observed between the sexes of grey seal but the number of worms increased with the weight of the seals. Abundance of P. decipiens and C. osculatum showed geographical and seasonal differences. Seals from the Breidafjord and Faxafloi areas had greatest abundance of P. decipiens with an increasing trend from spring to autumn, reaching the highest levels during period of reduced feeding in the breeding season in October to December. Abundance of C. osculatum was greatest in seals from the Breidafjord area and decreased from spring to autumn. The abundance was low in seals from the Faxafloi area and remained unchanged from spring to autumn. Most samples from the south coast were collected from August to December and showed low and declining abundance of all anisakid species during the breeding season. No indications of changes in P. decipiens abundance were observed in grey seals from West Iceland between 1979–82 and 1989–93. Proportions of adult P. decipiens and C. osculatum showed a decreasing trend when abundance of each species in the seal stomachs was increasing.
- Published
- 1997
- Full Text
- View/download PDF
25. Anisakid Nematodes and Anisakiasis
- Author
-
F.Y. Cheah and Alan J. Lymbery
- Subjects
Anisakidae ,Larva ,Anisakid nematode ,biology ,Ecology ,Raphidascarididae ,Paratenic ,Anisakis simplex ,%22">Fish ,biology.organism_classification ,Invertebrate - Abstract
Anisakiasis (anisakidosis) refers to infection of people with larval stages of ascaridoid nematodes belonging to the family Anisakidae (and possibly also Raphidascarididae). These worms, commonly called anisakids, utilize aquatic mammals, piscivorous birds, aquatic reptiles, or fish as definitive hosts, and aquatic invertebrates and fish as intermediate or paratenic hosts.
- Published
- 2007
- Full Text
- View/download PDF
26. Biological observation on a dwarf minke whale (Balaenoptera accutorostrata), caught in southern Brazilian waters, with a new record of prey for the species
- Author
-
Alexandre N. Zerbini, Eduardo R. Secchi, Lauro Barcellos, and Luciano Dalla Rosa
- Subjects
Bycatch ,Immature male ,Fishery ,geography ,Euphausia similis ,geography.geographical_feature_category ,Anisakid nematode ,Balaenoptera ,biology ,Continental shelf ,Minke whale ,biology.organism_classification ,Predation - Abstract
In the late austral spring, a 3.43m long immature male dwarf minke whale ( Balaenoptera accutorostrata ) was accidentally caught in a gillnet set in waters 143m deep, close to the continental slope off southern Brazil. A brief description of the specimen, including external measurements, colour patterns, reproductive status and skull measurements, is presented. The stomach was full of the euphausiid, Euphausia similis , and was heavily infested by anisakid nematode parasites. Euphausia similis is reported for the first time as a prey species for dwarf minke whales.
- Published
- 2003
- Full Text
- View/download PDF
27. Pathology and Differential Diagnoses of Internationalized Zoonoses: Summarizing Discussion of the Organizers
- Author
-
Hideyo Itakura, Hajime Ishikura, and Masamichi Aikawa
- Subjects
Pathology ,medicine.medical_specialty ,Anisakid nematode ,Hysterothylacium aduncum ,business.industry ,medicine ,Medical diagnosis ,business ,humanities - Abstract
This chapter presents a summary of eight lectures of Symposium 6 of the 86th Annual Meeting of the Japanese Society of Pathology, held in Sapporo, Japan, June 4–6, 1997. Pathological aspects of parasitic diseases and related topics were discussed in the symposium, including host- to-parasite relationships and imported parasitic disorders. Pathological, diagnostic, and preventive topics of imported zoonoses were vigorously discussed. The discussions concerning these important problems were very much appreciated by the members of the Japanese Society of Pathology.
- Published
- 1998
- Full Text
- View/download PDF
28. Prevalence of Larval Anisakid Nematodes in Fresh Fish from Coastal Waters of Hokkaido
- Author
-
K. Miyamoto
- Subjects
Fishery ,Larva ,Anisakid nematode ,Fresh fish ,Anisakis simplex ,Marine fisheries ,%22">Fish ,Biology - Abstract
Five hundred and forty eight fish consisting of 19 species were purchased from the Kyokuichi (commercial marine fishery) in Asahikawa city, Hokkaido. They were dissected and examined for anisakid larvae in the body muscle and in the viscera.
- Published
- 1990
- Full Text
- View/download PDF
29. Human Infections with Pseudoterranova cattani Nematodes, Chile.
- Author
-
Weitzel T, Sugiyama H, Yamasaki H, Ramirez C, Rosas R, and Mercado R
- Subjects
- Adult, Animals, Ascaridida Infections epidemiology, Ascaridida Infections transmission, Ascaridoidea pathogenicity, Chile epidemiology, Female, Fish Diseases epidemiology, Fish Diseases transmission, Fish Products parasitology, Humans, Larva pathogenicity, Male, Middle Aged, Ascaridida Infections parasitology, Ascaridoidea genetics
- Published
- 2015
- Full Text
- View/download PDF
30. PHOCANEMA, A NEW GENUS FOR THE ANISAKID NEMATODE OF SEALS
- Author
-
Betty J. Myers
- Subjects
Anisakid nematode ,Ecology ,Genus ,Ascaris ,Helminths ,Zoology ,Animal Science and Zoology ,Taxonomy (biology) ,Biology ,biology.organism_classification ,Ecology, Evolution, Behavior and Systematics - Abstract
The anisakid nematode originally called Ascaris decipiens Krabbe, 1878, the adults of which occur in seals in the Atlantic off the eastern coast of Canada, is removed from the genera Porrocaecum and Terranova in which it has been variously placed and is made the type and only species of the new genus Phocanema. The hosts of the genus Porrocaecum are birds and the hosts of the genus Terranova are elasmobranchs.
- Published
- 1959
- Full Text
- View/download PDF
31. The occurrence of anisakid nematode larvae in Baltic cod (Gadus morhua callarias L.) and the dynamics of their invasion
- Author
-
Jadwiga Grabda
- Subjects
Larva ,Anisakid nematode ,fungi ,Gadus ,Zoology ,Aquatic Science ,Biology ,biology.organism_classification ,humanities - Abstract
Larvae of two nematode species: Anisakis simplex (Rud.) and Contracaecum aduncum (Rud.) were found to occur in Baltic cod (Gadus morhua callarias L.). Their occurrence in various fish organs was analysed; the investigations covered also the invasion incidence fish size relationship, the annual variability of infestation and its occurrence in different regions of the Southern Baltic.
- Published
- 1976
32. The histopathology of Scomber japonicus infection by Nematobothrium scombri (Trematoda:Didymozoidae) and of larval anisakid nematode infections in the liver of Pagrus pagrus
- Author
-
A. Arandas Rego and J. C. Eiras
- Subjects
Microbiology (medical) ,Scomber ,medicine.medical_specialty ,Larva ,biology ,Liver Diseases, Parasitic ,Zoology ,Pagrus ,biology.organism_classification ,Fishery ,Fish Diseases ,Nematode ,Anisakid nematode ,medicine ,Animals ,Histopathology ,Trematoda ,Nematode Infections ,Didymozoidae - Abstract
The histopathology induced by Nematobothrium scombri (Trematoda) in Scomber japonicus and of larval anisakid nematodes in Pagrus pagrus is described. Nematodes larvae occurred within the liver capsule and N. scombri occurred within nodules in the opercula. The fishes were collected off the coast of Rio de Janeiro State.
- Published
- 1987
33. An anisakid nematode larva with aberrant appendix
- Author
-
Bjørn Berland
- Subjects
Fishery ,Larva ,Anisakid nematode ,medicine.anatomical_structure ,Ecology ,Phocascaris ,parasitic diseases ,fungi ,medicine ,Aquatic Science ,Biology ,Appendix ,Theragra chalcogramma - Abstract
An anatomical anomaly involving reversed (anterior) direction of the oesophageal appendix in an anisakid (Contracaecum or Phocascaris sp.) larva, collected from Theragra chalcogramma in British Columbia, Canada, coastal waters, is described and illustrated.
- Published
- 1981
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.