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SUMMARY Sorghum bicolor (sorghum) is a vital C4 monocotyledon crop cultivated in arid regions worldwide, valued for its significance in both human and animal nutrition. Despite its agricultural prominence, sorghum research has been hindered by low transformation frequency. In this study, we examined sorghum transformation using the pVS1‐VIR2 ternary vector system for Agrobacterium, combined with the morphogenic genes BABY BOOM and WUSCHEL2 and selection using G418. We optimized Agrobacterium‐mediated infection, targeting key parameters such as bacterial optical density, co‐cultivation time, and temperature. Additionally, an excision‐based transformation system enabled us to generate transgenic plants free of morphogenic regulators. The method yielded remarkable transformation frequencies, reaching up to 164.8% based on total isolated plantlets. The same combination of ternary vector, morphogenic genes and geneticin‐based selection also resulted in a marked increase in transformation efficiency of the Zea mays (maize) inbred line B104. The potential for genomic editing using this approach positions it as a valuable tool for the development of sorghum and maize varieties that comply with evolving European regulations. Our work marks a significant stride in sorghum biotechnology and holds promise for addressing global food security challenges in a changing climate. [ABSTRACT FROM AUTHOR]

Lucerne hay is undoubtedly one of the most important forage crops used in animal nutrition globally. This article sheds light on the intricacies of the South African lucerne hay sector by offering an industry overview. Lucerne hay quality is categorised into different grades: Supreme, Prime, Grade 1, Grade 2, and Grade 3, each exhibiting its distinct characteristics and attributes based on the nutritional value of dairy cattle. The producer price of lucerne hay is driven by supply and demand in the market. These fluctuations reflect the attractiveness of new producers entering or producers exiting the industry, indicating the sensitivity of market dynamics to external factors like weather and international demand. On average, South Africa exports 160 to 240 thousand tons annually, mainly to neighbouring countries and the Middle East. Over the last several years, the Chinese markets imported a growing volume of South African-produced lucerne hay. Weather conditions are one of the leading drivers of changes in the availability, quantity, and quality of lucerne hay in South Africa, impacting the producer price of lucerne hay in the local market. This article aims to provide insight into the dynamics of the South African lucerne hay industry. Additional research is required to understand the interactions of weather conditions (temperature variations, rainfall and occurrence of rainfall) on lucerne hay production and quality. Understanding international demand for South African lucerne hay will provide insight into the competitiveness of South African lucerne hay in the international market. [ABSTRACT FROM AUTHOR]

The re-integration of former foodstuffs, such as bakery products (BP), into pig feed reduces the feed-food competition and increases the sustainability of pig production. Nevertheless, there are concerns about the altered fatty acid profile in BP compared to standard diets and their effects on meat quality and the intestinal microbiome. The present study investigated the effects of replacing 30% cereal grains with BP in the diet on performance, microbiome composition and metabolites in feces of fattening pigs, economic aspects and meat quality characteristics. A total of 132 pigs (Large White × Piétrain; 11 weeks of age) were fed two diets containing 0 or 30% BP until reaching slaughter weight in three replicate batches. Fecal samples were collected for 16S rRNA amplicon sequencing after 6 weeks, whereas body fat samples were collected after 9 weeks on the diet. The results showed that BP can replace cereals in pig diets by up to 30% without impairing animal performance, while improving feed efficiency and reducing feed costs by 9% compared to the control. Additionally, pigs fed the BP diet had a 50%-higher n-3 fatty acid content in the neck and abdominal fat compared to the control. The improved feed efficiency in BP-fed pigs may be related to positive effects on the fecal microbiome, such as a higher alpha diversity and a reduction of fungi and yeasts. Our study provides valuable insights into the benefits of including BP in diets of fattening pigs, which should be investigated in future studies on other BP types. [ABSTRACT FROM AUTHOR]

Lactic acid bacteria (LAB) probiotics are health-promoting but their characteristics, safety profile and functional mechanisms are not fully understood. Hence, this study aimed to characterize some hypothetical proteins of the chicken-isolated Limosilactobacillus reuteri genome and unravel their IL-2 and IL-10-inducing potential to understand mechanisms of their immunological functionality for sustainable applications. The selected proteins were subjected to in silico analyses for transmembrane topology, sub-cellular localization, IL-2 and IL-10-inducing ability and IL-2 and IL-10 gene expression across various conditions. IL-2 and IL-10-inducing mutants were statistically analyzed using a one-way analysis of variance of a general linear model of SAS and statistical significance was set at p < 0.05. The analyzed proteins are stable under a wide temperature range. All the hypothetical proteins are IL-2 and IL-10-inducing but QHPv.2.12, QHPv.2.13 and QHPv.2.15 are non-immunogenic. The evaluated mutants are IL-2 and IL-10-inducers but QHPv.2.13 and QHPv.2.15 are not immunogenic. This study sheds light on understanding the functional mechanisms of chicken-isolated L. reuteri and suggests it or its proteins as potent candidates for feed additive and therapeutic purposes. [ABSTRACT FROM AUTHOR]

ABSTRACT Insects are emerging as a promising alternative source in animal nutrition, offering high protein content and a low environmental impression compared to traditional feed sources. This abstract explores the potential role of insects in reducing the environmental impact of livestock production. Insects such as black soldier fly larvae, mealworms, and crickets are rich in essential nutrients, including proteins, amino acids, fatty acids and so on. They can be efficiently farmed on organic waste streams, contributing to resource efficiency and waste lessening. Additionally, insect farming needs a small quantity of water, less land, and emits fewer greenhouse gases than conventional animal feed production. Despite these benefits, several challenges must be addressed to realize their potential, including regulatory approval, consumer acceptance, production scalability, and ensuring consistent nutritional quality. By overcoming these challenges through research, innovation, and policy support, insects can be integrated into mainstream animal feed systems, significantly reducing the environmental impact of livestock farming and contributing to a more sustainable agricultural future. [ABSTRACT FROM AUTHOR]

Following a request from the European Commission, EFSA was asked to deliver a scientific opinion on the safety and efficacy of l‐valine produced by fermentation with a genetically modified strain of Escherichia coli (CGMCC 22721) as a nutritional additive for all animal species. The production strain and its DNA were not detected in the final additive. Therefore, the final product does not give raise to any safety concern regarding the genetic modification of the production strain. The EFSA Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) concludes that l‐valine produced using E. coli CGMCC 22721 is safe for the target species when supplemented in appropriate amounts to the diet according to the nutritional needs of the target species. The FEEDAP Panel has concerns on the use of amino acids in water for drinking for hygienic reasons, and due to the risk of imbalances when administered simultaneously via feed. The use of l‐valine produced using E. coli CGMCC 22721 in animal nutrition is considered safe for the consumers and for the environment. The FEEDAP Panel cannot conclude on the potential of l‐valine produced using E. coli CGMCC 22721 to irritant to the skin or eyes, a dermal or respiratory sensitiser due to the lack of data. The endotoxin activity of the additive does not represent a hazard for users handling the additive when exposed by inhalation. The additive l‐valine produced by fermentation using E. coli CGMCC 22721 is regarded as an efficacious source of the essential amino acid l‐valine for non‐ruminant nutrition. For the supplemental l‐valine to be as efficacious in ruminants as in non‐ruminant species, it requires protection against degradation in the rumen. [ABSTRACT FROM AUTHOR]

Following a request from the European Commission, EFSA was asked to deliver a scientific opinion on the safety and efficacy of a Camellia oleifera C.Abel seed extract (Cosap®) as technological feed additive for all animal species except fin fish. In the absence of adequate tolerance studies in the target species or toxicological studies with the additive under assessment, the EFSA Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) could not conclude on the safety of C. oleifera extract for the target species. The use of the additive in animal nutrition is not expected to cause concern for consumer safety. The additive is considered as irritant to the eyes and mucous membranes. No conclusions can be reached on the potential of the additive to be irritant to the skin or to be a dermal sensitiser. The use of the additive under the proposed conditions of use is considered safe for the terrestrial compartment. However, no conclusion can be reached on the safety of the additive for the environment when used in feed of aquatic animals other than fin fish. The Panel concluded that the additive has the potential to be efficacious as an emulsifier when used according to proposed conditions of use. [ABSTRACT FROM AUTHOR]

Mycotoxins are a major threat to animal and human health, as well as to the global feed supply chain. Among them, aflatoxins, fumonisins, zearalenone, T-2 toxins, deoxynivalenol, and Alternaria toxins are the most common mycotoxins found in animal feed, with genotoxic, cytotoxic, carcinogenic, and mutagenic effects that concern the animal industry. The chronic negative effects of mycotoxins on animal health and production and the negative economic impact on the livestock industry make it crucial to develop and implement solutions to mitigate mycotoxins. In this review, we summarize the current knowledge of the mycotoxicosis effect in livestock animals as a result of their contaminated diet. In addition, we discuss the potential of five promising phytogenics (curcumin, silymarin, grape pomace, olive pomace, and orange peel extracts) with demonstrated positive effects on animal performance and health, to present them as potential anti-mycotoxin solutions. We describe the composition and the main promising characteristics of these bioactive compounds that can exert beneficial effects on animal health and performance, and how these phytogenic feed additives can help to alleviate mycotoxins' deleterious effects. [ABSTRACT FROM AUTHOR]

Animal feed production has recently received significant attention in the food and livestock sectors due to its high nutritional value and substantial environmental potential. Extensive studies have been conducted to explore the effects of solid fermented feeds on different growth stages of animals but also on the health status of animals, the quality of animal products, and the composition of intestinal micro-flora to replace non-fermented feed production. The purpose of this review is to provide up-to-date existing trends, recent developments, and prospects of solid-state fermentation (SSF) practices for the production of animal feeds. Studies on enhancing nutritional factors by increasing the crude protein content, enzymes, and antioxidant activity of feed using physical processing methods on agro-industrial waste such as rapeseed meal, cottonseed, wheat bran, soybean meal, and legumes by mainly SSF are reviewed and discussed thoroughly. [ABSTRACT FROM AUTHOR]

Simple Summary: Age at puberty is a main factor affecting reproductive efficiency and, thus, livestock production. Besides species and breed, nutrition during growth plays a crucial role in the attainment of puberty. Controversial results are reported about the critical period during which heifers are more sensitive to the effects of nutrition on pubertal acceleration. Numerous papers are available about such topics, but they are characterized by very different experimental conditions (such as genetics, feeds, planes of nutrition, season and pasture quality, time windows, and parameters considered), making difficult the comparison among inconstant results by various trials. Several indicators during growth have been proposed as predictors of age at puberty, such as body weight gains, body fat reserves, and linear body measurements. Blood concentrations of hormones strictly linked to growth and reproduction have also been considered: higher Insulin-like Growth Factor-1 levels are reported to be related to earlier puberty, while leptin is not. More uniform experimental conditions should be encouraged for future research. Puberty onset and age at first calving have a critical impact on livestock production for good reproductive efficiency of the herd and to reduce the duration of the non-productive stage of the growing heifer. Besides genetic factors, sexual maturation is also affected by environmental factors, such as nutrition, which can account for up to 20% of the observed variability. The rate of body weight gain during growth is considered the main variable influencing the age at puberty, dependent on planes of nutrition in growing animals during the prepubertal-to-pregnancy stage. This paper reviews current knowledge concerning nutrition management and attainment of puberty in heifers, considering the relevance of some indicators such as body measurements and hormones strictly linked to the growth and puberty process. Puberty onset is dependent on the acquisition of adequate subcutaneous adipose tissue mass, as it is the main source of the hormone leptin. Until a certain level, body condition score and age at puberty are negatively correlated, but beyond that, for fatter animals, such correlation is gradually lost. Age at puberty in heifers was reported to be negatively related to IGF-1. Future research should be planned considering the need to standardize the experimental animals and conditions. [ABSTRACT FROM AUTHOR]

Context: Proximate analysis is also called the Weende method. It has been the standard used for describing the chemical composition of feed ingredients for the past 150+ years. Understanding of the chemical composition of feeds has changed greatly in that time. Most of the current terms used for proximate analyses are unclear and misleading: (1) Crude protein , nitrogen × 6.25 describes true protein plus any other nitrogen-containing compounds including nucleotides and even phospholipids. (2) Crude fibre represents approximately half of the true fibre in most ingredients, but only approximately one-seventh of the fibre in important ingredients like soybean meal. (3) Ether Extract represents the neutral lipids in ingredients but little of the polar lipids like the lecithins. (4) The Nitrogen-free Extract is not an extract at all. It is supposed to represent starch in feed, but it contains large proportions of pectin, hemicellulose and even some cellulose. It reflects the error in Crude Fibre. Inadequacies of the Weende system have been known for a very long time. Animal producers desire to embrace modern technology and adapt more precise feeding techniques. Aims: This paper explains how 19th century chemistry relates to 21st century understandings of feed composition and proposes an updated method of feed ingredient analysis. Methods: A new method of describing feed composition based on 13 modern chemical categories was conceived and called the 'Armidale Method', to distinguish it from the Weende method. The new feed chemical category method was used to compile a preliminary database based on: moisture, sugars, oligosaccharides, starch, pectin, hemicellulose, cellulose, lignin, ash, neutral lipids, polar lipids, true protein, and nonprotein nitrogenous compounds. Key results: Composition values for 26 ingredients compiled from three sources averaged 1032.0 ± 49.5 g/kg, comparable to the theoretical 1000 g/kg. Conclusions: The Armidale Method of analyses could be the starting point for discussions of new standardised procedures for ingredient trade and feed formulation. Implications: It is time for producers, in conjunction with nutritionists and analytical chemists, to explore the best ways to represent the composition of feed ingredients for feeding value and trade. Since the 19th century, when little was known about chemical composition of feeds, gross feed analysis for trade and estimating energetic contributions has been based on the Weende method (moisture, crude protein, crude fibre, ether extract, ash and nitrogen-free extract). A new, Armidale Method, is proposed here. It consists of 13 chemical categories based on modern knowledge of feed chemistry (moisture, true protein, nonprotein nitrogenous compounds, neutral and polar lipids, sugars, oligosaccharides, starch, pectin, lignin, hemicellulose, cellulose, and ash). This paper belongs to Animal Science Reflections and Collection: Recent Advances in Animal Nutrition - Australia 2025. [ABSTRACT FROM AUTHOR]

The aim was to evaluate the influence of residual feed intake (RFI) on the semen characteristics of Dorper sheep after cryopreservation. Six Dorper sheep were used, classified from a population of 64 according to feed efficiency (groups of animals-positive RFI and negative RFI). The ejaculates were harvested three times a week, with a minimum interval of 48-h between collections, being seven collections per animal, totalling 42 ejaculates. Motility, plasma membrane integrity, acrosomal, mitochondrial activity and perivitelline membrane binding tests were performed. Negative RFI animals presented higher progressive motility (31.28%) than of RFI positive (25.37%). Negative and positive RFI animals present similar qualities in the assessment of plasma and acrosomal membrane integrity and mitochondrial activity, and negative RFI animals presented a higher number of membrane-bound spermatozoa than the positive RFI animals. Negative RFI animals displayed better rates of sperm motility after thawing, and are more efficient in the fertilisation in vitro. RFI did not influence the integrity of the plasma and acrosomal membrane, nor the mitochondrial activity of Dorper sheep spermatozoa after cryopreservation. [ABSTRACT FROM AUTHOR]

Balanced bacterial metabolism is essential for cell homeostasis and growth and can be impacted by various stress factors. In particular, bacteria exposed to metals, including the nanoparticle form, can significantly alter their metabolic processes. It is known that the extensive and intensive use of food and feed supplements, including zinc, in human and animal nutrition alters the intestinal microbiota and this may negatively impact the health of the host. This study examines the effects of zinc (zinc oxide and zinc oxide nanoparticles) on key metabolic pathways of Escherichia coli. Transcriptomic and proteomic analyses along with quantification of intermediates of tricarboxylic acid (TCA) were employed to monitor and study the bacterial responses. Multi-omics analysis revealed that extended zinc exposure induced mainly oxidative stress and elevated expression/production of enzymes of carbohydrate metabolism, especially enzymes for synthesis of trehalose. After the zinc withdrawal, E. coli metabolism returned to a baseline state. These findings shed light on the alteration of TCA and on importance of trehalose synthesis in metal-induced stress and its broader implications for bacterial metabolism and defense and consequently for the balance and health of the human and animal microbiome. [ABSTRACT FROM AUTHOR]

A large-scale study was conducted to explore AFB1 contamination in feed samples using Thin Layer Chromatography, following an AOAC protocol. Samples were identified which were not compliant with the maximum limits for AFB1 as regulated in the United States and Pakistan. Of a total of 923 samples, 51 samples (5.5%) were not compliant according to Pakistan and 267 (28.9%) were not compliant with US-FDA standards. The overall prevalence of non-compliant samples of compound feed was 26.2% (n = 117) according to US-FDA standards, while none of the samples were non-compliant according to Pakistan standards. Among feed ingredients, the overall prevalence of non-compliant samples was 10.7% (n = 51) and 31.4% (n = 150) according to Pakistan and USFDA standards, respectively. Non-compliant feed with respect to AFB1 contamination was highly prevalent in Khyber Pakhtunkhwa, posing a serious threat to production performance and animals health. [ABSTRACT FROM AUTHOR]

The use of imported soybean/soybean meal is criticized in the context of the sustainability discussion in Germany. Imported soybean is often associated with deforestation and genetic modification, which is largely viewed critically by consumers. This study aims to forecast changes in future demand for imported soybean/soybean meal and its possible substitutes in the German livestock industry. The two-round Delphi method was used in the study by sending questionnaires to 28 experts from four groups: food retailers, livestock associations, animal nutrition manufacturers and research institutes. Our main result is that the total use of soybeans in German livestock farming will decrease from almost 4 million tons of soybean equivalents in 2018/19 to approximately 3.4 million tons by 2030/31. In contrast, the share of non-GM soybean is forecast to increase from 26 to 53%. Factors that influence the increased use of non-GM protein feeds most are "specifications from downstream processors" and "demand from the feed industry." Experts forecast that about 36% of imported soybean/soybean meal (from non-EU countries) for German livestock farming could be replaced by protein feed produced in the EU. Rapeseed was considered to have a particularly high potential for production in the EU, followed by soybean and sunflower. Experts considered the factors: "requirements by the food retailers," "reasonable price," "political regulation" and "better profitability for the producer" as the most influential for extended use of regional protein feeds. [ABSTRACT FROM AUTHOR]

[Objective] The effects of Spanish alfalfa replacing American alfalfa on performance, serum biochemical indexes and rumen microflora of East Friesian and Hu sheep crossbreed first-generation dairy sheep (DHF1) were studied. [Method] Forty-eight DHF1 sheep (second parity and second lactation month) were randomly divided into two groups, with 6 replicates in each group and 4 sheep in each replicate. After 15 days of pre-feeding, the formal experiment was conducted for 45 days. During the trial period, the two groups of sheep were fed on silage corn and pellet feed (alfalfa, oat and concentrate) with the same ingredients except for different alfalfa types.The feed intake and residual feed were recorded every day, and milk yields by machine at 10:00 and 18:00 every day were measured during the experimental period. On days 15, 30 and 45 of the trial period, small amounts of milk samples were taken to determine routine nutritional components (milk fat, milk protein, lactose and non-fat solids). On day 45 of the trial period, 10 mL blood was taken from jugular vein to determine serum biochemical indexes (total protein, albumin, globulin, total cholesterol, triglyceride, high-density lipoprotein, low-density lipoprotein, blood sugar, malondialdehyde, aspartate aminotransferase, alanine aminotransferase, superoxide dismutase, glutathione peroxidase activity and total antioxidant capacity). Rumen fluid of dairy sheep from each group was collected by rumen fluid collection tube on day 45 of the trial period, and rumen microflora was analyzed by 16S rDNA.Finally, economic benefits of the two experimental groups were analyzed. [Result] There was no significant difference in milk yield between the two groups on days 15, 30 and 45 (P>0.05). On day 30 of the trial period, the fat of dairy sheep in Spanish alfalfa group was significantly higher than that in American alfalfa group (P<0.05), and there was no significant difference among other indexes (P>0.05). The content of total protein in serum of Spanish alfalfa group was significantly higher than that of American alfalfa group (P<0.05). Chao1 index, Shannon index and Shannon/Simpson index of rumen microorganisms in Spanish alfalfa group were significantly higher than those in American alfalfa group (P<0.05), Simpson index was not significantly different from that in American alfalfa group (P>0.05), and coverage was significantly lower than that in American alfalfa group (P<0.05). The dominant bacteria in rumen of test sheep in the two groups were Firmicutes and Bacteroides, and the relative abunclance of Proteus in rumen of the American alfalfa group was significantly higher than that of the Spanish alfalfa group (P<0.01). At the genus level, there was no significant difference in the relative abundance of each genus in rumen between the two groups (P>0.05). The daily economic benefit of dairy sheep in Spanish alfalfa group was slightly higher than that in American alfalfa group. [Conclusion] Spanish alfalfa instead of American alfalfa has no negative effect on performance, serum biochemical indexes and rumen health of dairy sheep. [ABSTRACT FROM AUTHOR]

Simple Summary: The prickly pear (Opuntia ficus indica L.) is a hardy plant well-adapted to hot and arid environments, rich not only in fiber but also soluble carbohydrates, lipids, minerals, and other antioxidant compounds. It has recently attracted attention for its potential uses in human and animal nutrition, however its effects on rabbits are still poorly understood. Its incorporation into the rabbit diet as a source of fiber would reduce the use of alfalfa with several benefits for the sustainability of rabbit farming from the point of view of the circular economy, global warming, and meat production costs. This study investigated the use of prickly pear seed cake in the rabbit's diet with different inclusion percentages (10% and 20%). Various parameters were evaluated, including growth performance, nutrient digestibility, blood biochemical parameters, morphology of intestinal villi, and carcass characteristics. While productive parameters were similar, diets containing prickly pear seed cake led to improved digestive utilization of nutrients, as well as reduced blood lipid concentrations and fat content in the rabbit carcasses. The encouraging findings of our study suggest that the inclusion of prickly pear in rabbit feed is feasible, although further research is necessary to validate the current outcomes and to evaluate the economic convenience of its use. Prickly pear (Opuntia ficus indica L.) could be used in rabbit nutrition in compliance with circular economy principles, global warming issues, and reduction of production costs. This study aims to evaluate the effects of dietary incorporation of prickly pear seed cake (PPSC) on growth, physiological, and histometric parameters in rabbits. A total of 105 rabbits were divided into three experimental groups (n = 35) and fed different diets: a commercial feed (C group), the same feed with alfalfa replaced by PPSC at 10% (10PP group), and at 20% (20PP group). They were group-housed in cages with 5 animals per cage from weaning until slaughtering. While body weights and weight gains were similar in all groups, the coefficients of nutrient digestibility of dry matter, fibers, and ashes, as well as the characteristics of intestinal villi, were improved in the 10PP group compared to the others (p < 0.05). The 20PP group showed a reduction in perirenal and interscapular fat (p < 0.05), as well as lower plasma concentrations of triglycerides and cholesterol compared to the C group (p < 0.001). In conclusion, PPSC can be incorporated into the diets of growing rabbits up to 20% as a partial substitute for alfalfa without the impairment of growth performance. Additionally, the inclusion of PPSC enhanced nutrient digestibility and increased the intestinal absorption surface area. [ABSTRACT FROM AUTHOR]

Simple Summary: The integration of agro-industrial residues into animals' diet offers a major potential for advancing of a circular economy, enhancing both economic and environmental sustainability. Hempseed cake is widely utilized around the globe as a source of food and supplement ingredients. Hempseed cake has a pleasant nutty flavour and is a valuable source of essential fatty acids, minerals, vitamins, and fibres. It also contains essential amino acids within its highly digestible proteins. Feeding with a hempseed cake in rabbits, given at both tested concentrations, had no effect on weight gain per week and the total average weight gain compared to the control group. Hempseed cake addition did not decrease sperm concentration in ejaculate, sperm motility, and progressive motility. Selected haematological and biochemical indexes were examined. No negative effects of hempseed cake feeding on male rabbit reproduction and health status were found. Incorporating of agro-industrial co-products into animal nutrition could represent an opportunity to lessen the environmental impact of the food production chain. One such co-product is a hempseed cake originating from cold pressing hemp seeds to extract oil for human consumption. The aim of the present study was to evaluate the action of hempseed cake in the diet on male rabbit reproductive and some non-reproductive indexes. Male rabbits were fed either a standard diet (control group; C; n = 10) or a diet enriched with hempseed cake (experimental group E5 with 5% of a hempseed cake; n = 10, and experimental group E10 with 10% of a hempseed cake; n = 10) in 100 kg of the milled complete feed mixture. Rabbit weight gain, sperm concentration, motility, progressive motility, and sperm quality were evaluated using CASA and flow cytometry. Feeding with a hempseed cake, given at both tested concentrations, had no effect on weight gain per week and the total average weight gain compared to the control group (p > 0.05). Hempseed cake addition had no effect on sperm concentration in ejaculate, sperm motility, and progressive motility (p > 0.05). Selected haematological and biochemical indexes were examined. The E5 group showed positive tendencies in hepatic profile parameters, while in the E10 group the tendencies were opposite, though within the reference values. Based on our results, no negative effects of hempseed cake feeding on rabbit reproduction and health status were found, and we can recommend the use of hempseed cake at doses up to 10% in the nutrition and feeding of rabbits. Therefore, agro-industrial co-products can decrease the feeding cost. [ABSTRACT FROM AUTHOR]

We have studied the main directions of age-related changes and the parameters of some enzymes involved in energy metabolism in the mitochondria of organs under the influence of adverse factors and a protein-deficient diet. To elucidate the influence of physical factors and a protein-deficient diet on the activity of Krebs cycle enzymes in rats and growing rat pups. Experimental studies were performed on 293 white female and male rats for insemination of females, weighing 180-200 g. Animals were placed in cages for fertilization of females for 10-12 days. Females - during the period of fertilization, after childbirth and lactation, were affected by high temperature and a protein-deficient diet and at the end of the above periods, the rats were slaughtered by decapitation. Mitochondria were isolated from liver tissue using 0.25 M sucrose, 25 mM EDTA, 10 mM Tris HCl, pH 7.4 as the isolation medium. In conditions of high air temperature and protein deficiency in the diet, metabolic processes in the liver are disturbed almost in all periods of the study, which is expressed in a significant decrease in the activity of dehydrogenases, respiratory enzyme and ATPase. Under the influence of unfavorable environmental factors and protein deficiency in the diet of mother rats - during pregnancy, childbirth and lactation and during the growth period of rat pups, after suckling mother's breast milk and during the transition of young animals to independent nutrition, a decrease in the intensity of redox reactions was observed. [ABSTRACT FROM AUTHOR]

The objective of this study was to examine the effect of humic acid (HA) supplementation on the rumen fermentation and protozoal community in the rumen. For this purpose, four ruminally cannulated Simmental cows were randomly assigned in a replicated 4 × 4 Latin square design experiment to study the effect of HA dietary supplementation on feed utilization, rumen fermentation, and protozoal community for 84 days. The basal diet (BD) was composed of meadow hay (68.2% of dry matter [DM]), maize silage (17% of DM), and granulated feed mixture (14.9% of DM). There were four treatments, including the BD without additives (control diet, H0), the BD supplemented with 50 g HA/cow/day (H50 treatment), the BD supplemented with 100 g HA/cow/day (H100 treatment), and the BD supplemented with 200 g HA/cow/day (H200 treatment). HA supplementation did not affect the total or individual volatile fatty acid concentrations, the total protozoa, or the ruminal pH. However, HA at the dosage of 50 g/cow/day increased the NH3-N concentration and fecal nitrogen compared to the control (p < 0.001). HA supplementation also significantly impacted the abundance of individual protozoal genera in the rumen. The results of this study suggest that HA has potential in ruminants as a natural feed additive and may play a role in nitrogen metabolism and stabilizing the protozoal community without adverse effects on rumen fermentation. [ABSTRACT FROM AUTHOR]

Introduction: Despite progress in reducing antimicrobial use in the veterinary field, it is crucial to find alternatives to preserve effectiveness and limit antimicrobial resistance. In pig farming, pathogenic strains of E. coli are the main cause of gastrointestinal disorders and antibiotic use. In this field, algae represent an innovation in animal nutrition that aligns with livestock sustainability principles and provide a high content of functional molecules. Aim: The aim of this study was to evaluate the impact of an innovative dietary combination of Ascophyllum nodosum and Lithothamnium calcareum, on growth, duodenum gene expression, jejunum intestinal morphology, and serum oxidative status in F4+ Escherichia coli challenged piglets. Materials and methods: Forty-eight weaned pigs, aged 28 ± 2 days, were divided into two groups (n = 24 pigs/group): the control group was fed a commercial diet (CTRL), while the seaweeds group was fed a commercial diet supplemented with 1.5% A. nodosum and 0.5% L. calcareum for 27 days (ALGAE). After 13 days, 50% of animals in each group were challenged with a single dose of 108 CFU/dose of E. coli F4+, resulting in two infected groups (CTRL+ and ALGAE+, n = 12 pigs/group). Growth performance was assessed by measuring the individual body weight. At day 27, from six animals/group duodenum and jejunum sections were sampled for gene expression analysis via qRT-PCR and histological evaluation. Results and discussion: The results indicated a significantly higher body weight in the ALGAE+ group compared to CTRL+ after 7 days post-challenge (p < 0.0001). Jejunum morphology revealed lower villus height, villus width and villus height/crypt depth ratio in CTRL+ compared to ALGAE+ (p < 0.05) suggesting a protective effect of seaweeds on gut health. Conclusion: In conclusion, algae mixture exerted a protective effect against intestinal damage from E. coli F4+ infection proposing A. nodosum and L. calcareum supplementation as interesting strategy to support animal growth, enhance health and reduce antibiotic treatments in weaned piglets. [ABSTRACT FROM AUTHOR]

Simple Summary: Weaning is a critical period in the development of piglets. During the weaning period, piglets are prone to weight loss, diarrhea, and even death due to factors such as the environment, feeding management, feed type, and feeding method. Current research indicates that essential oils can positively impact animal nutrition and production due to their antimicrobial and antioxidant properties. However, essential oils are quickly metabolized in the upper gastro-intestinal tract, so that the concentrations remaining in the distal small intestine are far too low to improve the intestinal tract. Microencapsulation technology is a relatively new processing method for essential oils. However, there is a scarcity of studies investigating the effects of microencapsulated essential oils (MEO) on growth and intestinal structure in weaned piglets. Consequently, further research on the application of MEO in the diet of weaned piglets is warranted. In this study, we administered varying concentrations of MEO to assess its impact on ADG, ADFI, FCR, diarrhea score, intestinal structure, antioxidant capacity, and other factors in weaned piglets. The aim was to develop new feed additives to enhance intestinal health of weaned piglets. The study investigated the effects of microencapsulated essential oils (MEO) on the growth performance, diarrhea, and intestinal microenvironment of weaned piglets. The 120 thirty-day-old weaned piglets (Duroc × Landrace × Yorkshire, 8.15 ± 0.07 kg) were randomly divided into four groups and were fed with a basal diet (CON) or CON diet containing 300 (L-MEO), 500 (M-MEO), and 700 (H-MEO) mg/kg MEO, respectively, and data related to performance were measured. The results revealed that MEO supplementation increased the ADG and ADFI in weaned piglets (p < 0.05) compared with CON, and reduced diarrhea rates in nursery pigs (p < 0.05). MEO supplementation significantly increased the duodenum's V:C ratio and the jejunal villi height of weaned piglets (p < 0.05). The addition of MEO significantly increased the T-AOC activity in the jejunum of piglets (p < 0.05), but only L-MEO decreased the MDA concentration (p < 0.01). H-MEO group significantly increases the content of isobutyric acid (p < 0.05) in the piglet colon, but it does not affect the content of other acids. In addition, MEO supplementation improved appetite in the nursery and increased the diversity and abundance of beneficial bacteria in the intestinal microbiome. In conclusion, these findings indicated that MEO supplementation improves growth and intestinal health in weaned piglets. [ABSTRACT FROM AUTHOR]

Simple Summary: The European Union is working towards a sustainable future by implementing strategies to guide the agricultural transition towards fairer, healthier, and more environmentally friendly food systems, increasing their profitability and sustainability. As a result, food producers are looking for different and stronger products. (Cannabis sativa L.), due to its distinctive nutritional profile, can be considered an interesting and promising alternative resource for agriculture in human and animal nutrition. In this study, the proximate composition, fatty acid profile, antioxidant activity, total phenolic and N-trans-Caffeoyltyramine content were studied on three hemp varieties (Carmaenecta, Enectaliana, and Enectarol) grown in Central Italy. Enectarol had the highest total lipid content and the best antioxidant activity; Carmaenecta showed the best fatty acid profile and nutritional indices, and Enectaliana showed the highest crude protein and dietary fiber content. All the varieties revealed a high content of essential fatty acids (linoleic acid and α-linolenic acid) and oleic acid. In conclusion, the results highlight that hemp seeds can be used in the food industry as a source of oil and protein and as a supplement in feed mixtures for the valuable antioxidant activity and fatty acid profile, promoting better health in farm animals. The present research aimed to study the proximate composition, fatty acid profile, antiox-idant activity, total phenolic and N-trans-Caffeoyltyramine content of three distinct varieties of hemp seeds (Carmaenecta, Enectaliana and Enectarol, grown in a Mediterranean area (Central Italy), as feed in the diet of farm animals. Proximate composition was determined using the official methods of analyses; the fatty acid profile was determined by gas chromatography, total phenolic content (TPC) and the scavenging activity (DPPH• and ABTS•+) by the colorimetric method, and N-trans-Caffeoyltyramine content by HPLC analysis. The hemp seed Enectarol showed the highest total lipid content and the best antioxidant activity with the highest TPC, N-trans-Caffeoyltyramine content, and ABTS•+, and the lowest peroxidation index and DPPH•; Carmaenecta showed the best fatty acid profile and nutritional indices (atherogenic and thrombogenic indices and hypocholesterolemic/hypercholesterolemic ratio), and Enectaliana showed the highest crude protein and dietary fiber content. The differences observed in the chemical composition, fatty acid profile and antioxidant activity are because of the varieties, considering that all other growing conditions were the same. The results obtained suggest that hemp seed can be used as a source of lipid and protein in animal diets due to their valuable antioxidant activity and as a rich source of essential fatty acids. [ABSTRACT FROM AUTHOR]

Soybean is an economically important crop for animal and human nutrition. Currently, there is a lack of information on the effects of Trichoderma harzianum and Purpureocillum lilacinum on INTACTA RR PRO transgenic soybean plants. The present study evaluated the application of T. harzianum and P. lilacinum under field conditions. The results revealed a significant increase in soybean yield at 423 kg ha−1 in response to the application of P. lilacinum compared with the control treatment. In addition, the application of P. lilacinum promoted a significant increase in phosphorus levels in the plant leaves, and there were significant correlations between the increase in taxon abundance for the genus Erwinia and productivity and the average phosphorus and nitrogen content for the plant leaves, for the taxon Bacillus and nitrogen content and productivity, and for the taxon Sphingomonas and nitrogen content. The Bradyrhizobium taxon was identified in the P. lilacinum treatment as a taxon linking two different networks of taxa and is an important taxon in the microbiota. The results show that the application of the fungus P. lilacinum can increase the productivity of soybean INTACTA RR PRO and that this increase in productivity may be a function of the modulation of the microbiota composition of the plant leaves by the P. lilacinum effect. [ABSTRACT FROM AUTHOR]

This research was conducted at the experimental farm "Andil" of the State University of the South of Manabí, Cantón Jipijapa, Manabí, Ecuador, with the aim of evaluating the ethological behavior of the apparent ileal digestibility of dry matter (DM) and crude protein (CP) of Cobb 500 chickens in individual cages. 36 male chickens were used which were distributed in four treatments with different inclusion levels of sacha Inchi cake (SIC): T1 = control, without SIC, T2 = 10% SIC, T3 = 20% SIC and T4 = 30% SIC. A Completely Randomized Design (DCA) was used for the statistical analysis, where each treatment had 12 repetitions, locating one animal per experimental unit. The results determined that chickens had a good response in quickly adapting to the cages, in addition to not reporting mortality and accepting food. The apparent ileal digestibility of DM did not show a difference between the treatments, while the N differed between the control group and the inclusion of T4 with 30% (SIC), which was higher for the latter (85.20 vs 90.23%). The results allow to conclude that the ethological behavior of the animals is not affected and good indices of ileal digestibility of DM and N are obtained when adding SIC in the diet of broilers. [ABSTRACT FROM AUTHOR]

Objective. This systematic review examines the effects of essential oils used as phytonutrients in the diet of dairy animals, considering parameters such as feed consumption, total apparent digestibility, methane emissions and milk production, based on studies published between 2018 and 2023. Material and methods. Bibliographic research electronic method resulted in a careful selection of articles, which were reviewed and tabulated for evaluation. Results. The results show that 51.35% of the articles did not report significant effects of phytonutrients on milk production. However, 66.67% of the studies observed an increase in feed efficiency and 50% reported the presence of effects on apparent digestibility. Furthermore, there was a reduction in methane emissions in animals treated with phytonutrients. Conclusions. Sharing these results with dairy farming professionals can promote more sustainable and effective practices, as well as support future research and innovations in animal nutrition, aiming for both productive efficiency and environmental sustainability. [ABSTRACT FROM AUTHOR]

The increase in pineapple production has generated a large amount of waste, which, when incorporated into the soil, can lead to contamination and environmental degradation. In this context, this study highlights the innovative combination of pineapple waste and Leucaena hay, offering benefits for animal nutrition while reducing input costs. The objective of this study was to determine the nutritional value of silage from 'Pérola' pineapple waste (PS) with different levels of Leucaena hay. A completely randomized design was adopted with six treatments (pre-wilted PS and PS with 0%, 10%, 20%, 30%, and 40% inclusion of Leucaena hay (LH)). The dry matter content of the silage, crude protein, lignin, and pH increased linearly (p < 0.05) as a function of the inclusion of Leucaena hay, while buffering capacity, soluble carbohydrate content, and effluent losses showed an inverse relationship. As LH levels increased, ruminal DM disappearance decreased. Wilting the pineapple plant did not alter the in vitro gas production, in vitro digestibility of organic matter, partition factor (PF), and methane production when compared to the silages added with Leucaena hay. It is recommended to add 20% Leucaena hay in PS silage. [ABSTRACT FROM AUTHOR]

In prawn farming, the main protein source used in aquafeed formulations is fishmeal. Nevertheless, one estimates that in the coming years, this protein source will no longer be able to meet the demand for the activity. The search for new ingredients is important to meet the increasing demand and minimize environmental impacts, such as the reduction in fish stocks and the eutrophication of aquatic systems. The objective of this study was to determine the apparent digestibility coefficients of dry matter (DM), crude protein (CP), gross energy (GE), and ether extract (EE) of fishmeal, poultry co-products (viscera and hydrolysed feather), and insect meal (Gromphadorhina portentosa) for giant river prawn (Macrobrachium rosenbergii) juveniles. To determine the apparent digestibility coefficients (ADCs), 90 prawns (average weight, 15 g) were randomly distributed among three experimental units. The reference feed was formulated according to the requirements of the giant river prawn, with 35% crude protein and a gross energy of 3600 kcal kg−1. The test diets comprised 70% of the reference diet and 30% of the respective test ingredients. Prawns were fed three times a day until apparent satiety. Faeces were collected using the indirect siphoning method, twice a day at the same feeding site (at 7:30 a.m. and 6:30 p.m.). The water parameters were temperature (27 °C), dissolved oxygen (6.65 mg L−1) and pH (7.76). The ACDs of DM, CP, EE, and GE were, respectively, 61.48; 88.28; 99.89 and 88.25 for fishmeal; 76.48; 81.55; 97.29 and 85.13 for poultry viscera meal; 73.82; 75.21; 73.17 and 76.42 for hydrolysed feather meal; and 52.35; 59,48; 87.95 and 67.64 for G. portentosa meal. The values of protein (%) and digestible energy (kcal kg−1) were 55.20 and 3711 for fishmeal; 47.27 and 4285 for poultry viscera's meal; 65.03 and 4145 for hydrolysed feather meal; and 47.72 and 3616 for G. portentosa meal. These results showed the potential use of insect meals and poultry co-products as ingredients for the diets of M. rosenbergii juveniles, as they present digestible values close to those found for fishmeal, the main raw material used in aquaculture diets. [ABSTRACT FROM AUTHOR]

Simple Summary: Adding the phytoactive mixture to the dairy cows' diet positively affected animal nutrition and health, improving Jersey production efficiency. The intake of phytogenics improved milk quality, reduced somatic cell counts, and increased unsaturated fatty acids. The intake of phytogenics per cow protected the mammary gland from bacteria Streptococcus spp. The phytoactive mixture directly affected the digestibility and modulation of the volatile fatty acid profile. The phytoactive mixture positively affected immune and antioxidant responses and anti-inflammatory action. This study's objective was to evaluate whether adding a combination of phytoactive (microencapsulated essential oils, minerals, turmeric extract, tannin, prebiotic, and probiotic) to the feed of lactating Jersey cows positively affects the production, composition, and quality of milk, rumen environment, and animal health. Fourteen Jersey cows were divided into two groups (control and phytogenic) for an experiment with two lactation phases of 45 days each (early lactation and mid-lactation). During the experiment, milk production was higher at various times in cows that consumed phytoactive, and these animals had the best feed efficiency. In mid-lactation, phytoactive intake increased nutrient digestibility. The number of lymphocytes in the blood is reduced when cows consume phytoactive substances. Globulin levels increased in these cows fed with the additive, which may be related to a higher concentration of immunoglobulins, especially IgA. Cows fed phytoactives had lower ceruloplasmin and haptoglobin concentrations. Lower serum lipid peroxidation, associated with greater glutathione S-transferase activity, is a good health indicator in cows that consume phytoactive substances. The higher concentration of volatile fatty acids was due to the higher proportion of acetic acid in the ruminal fluid combined with lower butyric acid. Somatic cell counts in milk were lower in cows that consumed phytoactives during mid-lactation, as well as the effect of the treatment on Streptococcus spp. (lower in cows that consumed the additive). We conclude that consuming the additive benefits cows' health modulates rumen fermentation and nutrient digestibility, and positively affects milk production and quality. [ABSTRACT FROM AUTHOR]

Context: The planting and use of leucaena (Leucaena leucocephala) to fatten cattle is both practical and profitable for smallholder farmers in West Nusa Tenggara, Indonesia. Currently smallholder farmers feed leucaena as the primary component of a cattle fattening diet. However, the high protein content in leucaena could be more effectively used if combined with a fermentable energy source, such as cassava (Manihot utilissima). Aim: An experiment was conducted to determine the ratio of leucaena to cassava corresponding to largest average daily gain and most efficient feed conversion for gain. Methods: Thirty growing male Bali cattle (Bos javanicus) ~18 months of age with an initial live weight of 164 ± 1.8 kg (mean ± s.e.) were allocated to one of six experimental treatments in a randomised block design. Bulls were held in individual stalls and had access to experimental diets and water ad libitum. The diets were 20% rice straw + 80% leucaena hay (A), 20% rice straw + 65% leucaena hay + 15% cassava meal (B), 20% rice straw + 50% leucaena hay + 30% cassava meal (C), 20% rice straw + 35% leucaena hay + 45% cassava meal (D), 20% rice straw + 20% leucaena hay + 60% cassava meal (E) and 20% rice straw + 5% leucaena hay + 75% cassava meal (F). A mineral mix was provided at 1% DM of total diet and urea was added to the cassava meal at 2% DM. In consideration of animal welfare concerns, the trial was concluded on Day 77 due to observations indicating that bulls receiving the highest level of cassava (F) were experiencing significant weight loss. Key results: The optimum level of inclusion of cassava was 29.5% (based on the quadratic response curve) but there was little difference in average daily gain up to 45% inclusion. Feeding a high level of cassava meal (more than 45% of diet) reduced feed intake, average daily gain and income over feed cost. Conclusion: Cassava meal can be successfully and profitably incorporated into leucaena-based rations of Bali bulls for fattening. Implications: Formulating rations with cassava meal and leucaena can be economically beneficial in cattle fattening systems. Supplementing Bali cattle rations in leucaena-based smallholdings with locally available cassava as an energy source, provides an opportunity to increase cattle live weight gain, extend the utilisation of leucaena and increase farmer profitability. Weight gain and profitability were maximised up to 45% cassava, but higher levels reduced feed intake, average daily gain and income over feed costs. Feeding cassava meal to leucaena-based diets is an important strategy to preserve leucaena as well as to increase profitability and meat production nationally. [ABSTRACT FROM AUTHOR]

Simple Summary: Vitamins A and E are crucial for animal health, contributing to key functions such as vision, immune response, growth, reproduction, and neurological development. A deficiency in these micronutrients can lead to serious health issues. Supplementation, especially with innovative liquid formulations, is important for addressing these deficiencies and enhancing animal health and productivity. Administering these vitamins through drinking water offers a convenient method, especially during times of stress or increased nutritional demands. Injectable forms can enhance reproductive health, growth, and immune function in livestock, while liquid drops provide a precise and easy way to dose companion animals. Future research may focus on optimizing these formulations, developing specialized treatments, and leveraging nutrigenomics to customize animal nutrition, leading to advancements in veterinary care and livestock management. Vitamins A and E are vital fat-soluble micronutrients with distinct yet intertwined roles in various biological processes. This review delves into their functions, nutritional requirements across different animal species, the consequences of deficiencies, and the impact of liquid formulations on veterinary medicine and livestock production. Vitamin A exists in multiple forms, essential for vision, immunity, and growth, while vitamin E acts primarily as an antioxidant, safeguarding cell membranes from oxidative damage. Hypovitaminosis in these vitamins can lead to severe health consequences, affecting vision, immunity, growth, reproduction, and neurological functions. Hence, supplementation, particularly through innovative liquid formulations, becomes pivotal in addressing deficiencies and enhancing overall animal health and productivity. Injectable forms of vitamins A and E show promise in enhancing reproductive performance, growth, and immune function in livestock. Administering these vitamins through drinking water offers a convenient way to enhance livestock health and productivity, particularly during times of stress or increased nutritional needs. Liquid vitamin A and E drops offer a flexible and effective solution in veterinary practice, allowing precise dosing and easy administration, particularly for companion animals. Future research may aim to optimize formulations and explore targeted therapies and precision feeding via nutrigenomics, promising advancements in veterinary medicine and livestock production. [ABSTRACT FROM AUTHOR]

Recent attention has been given to animal feeding and its impact on human nutrition. Animal feeding is essential for meeting human dietary needs, making it a subject of significant interest and investigation. This review seeks to outline the current understanding of this disciplinary area, with a focus on key research areas and their potential implications. The initial part of the paper discusses the importance of animal feed resources and recognizes their crucial role in guaranteeing sufficient nutrition for both humans and animals. Furthermore, we analyzed the categorization of animal feeds based on the guidelines established by the National Research Council. This approach offers a valuable structure for comprehending and classifying diverse types of animal feed. Through an examination of this classification, we gain an understanding of the composition and nutritional content of various feedstuffs. We discuss the major categories of metabolites found in animal feed and their impact on animal nutrition, as well as their potential health advantages for humans. Flavonoids, polyphenols, tannins, terpenoids, vitamins, antioxidants, alkaloids, and essential oils are the primary focus of the examination. Moreover, we analyzed their possible transference into animal products, and later we observed their occurrence in foods from animal sources. Finally, we discuss their potential to promote human health. This review offers an understanding of the connections among the major metabolites found in feedstuffs, their occurrence in animal products, and their possible impact on the health of both animals and humans. [ABSTRACT FROM AUTHOR]

Successful sheep farming requires hands-on management and a thorough understanding of the factors that influence it. This necessitates a practical, well-organized approach to ensure the flock's health, productivity, and profitability. Various factors, such as climate, available resources, and management strategies, play a crucial role in determining the success of sheep farming. This study, conducted in the Batna region of Algeria, aimed to assess sheep breeding practices and production performance through a questionnaire distributed to farmers and veterinarians. The study encompassed 14,124 sheep, including 9,435 ewes, from 33 Batna region farms to evaluate production and reproduction performance. The findings revealed that the Ouled Djellal breed is the most prevalent, comprising 61.02% of the sheep population. The results indicated that sheep farming in the region largely relies on traditional extensive grazing systems. It is worth noting that breeding practices have not consistently followed recommended guidelines, as evidenced by a suboptimal sex ratio of 36.28. Additionally, only 21.94% of breeders had employed heat synchronization methods, and artificial insemination was not utilized. The low adoption of artificial insemination is primarily attributed to factors, including a lack of knowledge regarding reproductive management, along with challenges related to illiteracy and limited access to essential resources. The analysis further demonstrated that all categories of sheep were profitable; however, profitability was influenced by factors, such as environmental conditions, feed availability, and the age of the animals. The study underscored significant findings, including the prevalence of the Ouled Djellal breed and the limited use of advanced breeding practices, such as artificial insemination, in the Batna region. The value of the current study lies in its comprehensive examination of traditional sheep farming practices and its recommendations for enhancing productivity. These include improving management practices, increasing access to resources, and promoting genetic improvement by adopting advanced breeding technologies. [ABSTRACT FROM AUTHOR]

Simple Summary: Microencapsulated hot chili pepper (MHCP) has properties that affect the metabolism of bovines, so the objective of this research was to study the effects of MHCP on milk production and the reproduction of dairy cows. Twenty-four animals were separated into two groups. In the first group, each cow consumed 1 g of microencapsulated hot chili per day. The second group served as a control group for 42 days. Daily milk production and weekly milk composition were monitored for 42 days. These animals were subjected to a hormonal synchronization protocol, tests to monitor the size and vascularization of the ovarian structures, as well as the evaluation of serum progesterone. Ultimately, the supplemented group presented differences in milk production and milk components. On the other hand, a trend was found in which the ovarian follicle was mostly irrigated in the group that consumed the MHCP. We conclude that MHCP provides benefits in dairy production. The present study evaluates the effect of including microencapsulated hot chili pepper (MHCP) in the diet of crossbred dairy cows on the volume and quality of milk and on ovarian morphofunctionality. Twenty-four crossbred females in their lactating period were used. The cows were divided into two experimental groups, a control (CT) and an MHCP -supplemented group (CP) given 1 g a day per animal of microencapsulated hot chili in concentrate for 42 days. Over seven weeks of daily milk production was measured, and sample milk was collected weekly for composition analysis. Animals were subject to an ovulation synchronization protocol on day 0 (D0), and an intravaginal progesterone (P4) implant, estradiol benzoate, and prostaglandin (PGF2α) were administered. On D8, the P4 implant was removed and PGF2α, equine chorionic gonadotropin, and estradiol cypionate were administered to the animals. The ovarian dynamics were evaluated in B mode and color Doppler. There were significant differences (p < 0.05) in the group X time interaction, the volume of milk produced, and the amount in kg/day of milk components. There was a higher percentage of vascularization in the preovulatory follicle in the CP group (p ≥ 0.10). The findings show that the inclusion of MHCP in the diet of dairy cows does influence their milk production and reproduction. [ABSTRACT FROM AUTHOR]

Unconventional protein feeds, characterized by low nutritional value, high variability, and poor palatability, have limited their application in swine production. Fermentation technology holds the key to addressing these shortcomings. Given the ban on antibiotics in China, the inferior quality of imported pig breeds, and long-term dependence on imported soybean, the prospects for fermented unconventional protein feeds are promising. This paper delves into the common types of fermented unconventional protein feeds, factors influencing the fermentation process, the mechanisms by which they enhance swine health, and the challenges and prospects of fermented feeds, offering theoretical insights for the future development of the feed industry. [ABSTRACT FROM AUTHOR]

Wheat bran separated in the standard milling process as a by-product contains many substances of importance in livestock and human nutrition. In the Czech Republic, as in other Central European countries, a significant part of the bran is not traditionally used as a raw material for feed production and is used as a heating fuel. This means that many interesting and health-promoting components of fiber, phenolic compounds, vitamins, proteins, and minerals are lost. The bran is made up of particles of the grain outer coating and sub-coating layers, particularly the pericarp, testa, and aleurone layer. Their composition varies, but while the pericarp in particular is largely composed of cellulose and lignin, the testa and aleurone layer contain many valuable non-starch polysaccharides (hemicelluloses), as well as the macro- and micronutrients mentioned above. Wholemeal flours contain all the anatomical parts of the grain mentioned above, which brings both technological problems in terms of their bakery processing and a not always acceptable sensory impact on the products. This paper summarizes selected physical and physicochemical methods that can be used to remove those components that may cause technological and sensory problems and retain those that, on the other hand, represent a significant nutritional benefit. [ABSTRACT FROM AUTHOR]

There has been a growing interest in the use of hemp as an animal feed ingredient considering its economic value and nutritional properties. However, there is a paucity of research regarding the safety of hemp-based animal feed currently. Thus, this raises safety concerns on the potential transfer of cannabinoids from hemp-based animal feed to animal products intended for human consumption and its health effects. As such, the detection and quantification of cannabinoids in meat and animal feeds would be desirable for monitoring purposes. In this study, a simple, rapid and sensitive method for the simultaneous quantification of four major cannabinoids (delta-9-tetrahydrocannabinol, cannabidiol, cannabinol and tetrahydrocannabinolic acid) in meat and animal feeds by liquid chromatography–tandem mass spectrometry (LC-MS/MS) was successfully developed and validated. The method was selective and sensitive, achieving limits of detection and quantification for the four cannabinoids from 5 to 7 µg/kg and 15 to 21 µg/kg, respectively. The overall recovery with matrix-matched calibration curves for the cannabinoids ranged from 87–115%. The coefficients of variation were between 2.17–13.38% for intraday precision and 3.67–12.14% for inter-day precision. The method was subsequently applied to monitor cannabinoids in 120 meat and 24 animal feed samples. No cannabinoid was detected, suggesting no imminent food safety concerns arising from the potential incorporation of hemp and by-products in animal feed and nutrition under the promotion of sustainable agricultural practices. [ABSTRACT FROM AUTHOR]

Energy feed can provide animals with balanced nutrition, thereby enhancing their growth performance. This study aimed to evaluate the effects of dietary energy levels on the growth performance, serum metabolites, and meat quality of Jersey cattle–yaks. A total of 24 male Jersey cattle–yaks were randomly divided into three groups. Each group was fed diets with metabolizable energy levels of 8.21 MJ/kg (LE), 9.50 MJ/kg (ME), and 10.65 MJ/kg (HE), respectively. The HE and ME groups showed significantly higher final body weight, average daily gain (ADG), and feed efficiency compared to the LE group (p < 0.05). The glucose (GLU) and total cholesterol (TC) concentrations were significantly increased in the serum of the ME and HE groups (p < 0.05). The low-density lipoprotein cholesterol (LDL-C) and alanine aminotransferase (ALT) levels were significantly higher in the serum of the HE group than in the ME group (p < 0.05). Blood urea nitrogen (BUN) levels exhibited a significant decrease with increasing metabolizable energy levels in the diet (p < 0.05). Increasing dietary energy levels enhances the eye muscle area and intramuscular fat content of Jersey cattle–yaks (p < 0.05), with no effect on pH45 min, pH24 h, and shear force. In the HE group, the levels of heneicosanoic acid (C21:0), palmitoleic acid (C16:1), elaidic acid (C18:1n9t), and eicosadienoic acid (C20:2n6) were notably elevated (p < 0.05) when compared to the LE group. We concluded that a higher dietary energy level enhanced the growth performance and meat quality traits of male Jersey cattle–yaks. [ABSTRACT FROM AUTHOR]

Forage maize is a versatile crop extensively utilized for animal nutrition in agriculture and holds promise as a valuable resource for the production of fermentable sugars in the biorefinery sector. Within this context, the carbohydrate fraction of the lignocellulosic biomass undergoes deconstruction during ruminal digestion and the saccharification process. However, the cell wall's natural resistance towards enzymatic degradation poses a significant challenge during both processes. This so-called biomass recalcitrance is primarily attributed to the presence of lignin and ferulates in the cell walls. Consequently, maize varieties with a reduced lignin or ferulate content or an altered lignin composition can have important beneficial effects on cell wall digestibility. Considerable efforts in genetic improvement have been dedicated towards enhancing cell wall digestibility, benefiting agriculture, the biorefinery sector and the environment. In part I of this paper, we review conventional and advanced breeding methods used in the genetic improvement of maize germplasm. In part II, we zoom in on maize mutants with altered lignin for improved digestibility and biomass processing. [ABSTRACT FROM AUTHOR]

In this study, it was aimed to provide information about silage types, silage usage, feeding method of silage to dairy cows and solutions by organizing a survey in Karacabey district of Bursa city. A survey was applied to a total of 60 daily cow breeders, including large, medium, and small-scale enterprises. When asked “which type of silo do you use?”, 82% of the animal enterprises declared that they use bulk-type silage, while 10% declared that they use bank-type silage and 8% declared that they use bale-type silage. When the the animal enterprises were asked what they use as a silage additive, 70% of the breeders stated that they did not use additives in silage making, while 20% stated that they used bacterial inoculants plus enzymes in silage making, and %5 stated that they used yeast and the rest stated that they used enzymes. When the breeders were asked about the method of feeding the silage to dairy cows, 73.0% reported that they gave silage to their cows mixed with roughage and concentrate feed, 25.0% reported that they gave silage to their cows mixed silage with dry roughage and 2.0% reported that they gave only silage to their cows. As a result, it is seen that most of the breeders are dependent on maize for silage production and prefer the bulk-type silage. On the other hand, it has been determined that basic information about silage production and animal nutrition of small-scale livestock enterprises is insufficient. In this context, it can be recommended the small-scale enterprises to participate in training of organization which will increase their knowledges on subjects such as silage making and animal feeding. It may be suggested that they use other forage crops (food pulp residues and heat-resistant fodder crops) as a source of silage, and can also be fed the their animals daily by using a large bank type silage collectively. [ABSTRACT FROM AUTHOR]

Understanding biological mechanisms is fundamental for improving animal production and health to meet the growing demand for high-quality protein. As an emerging biotechnology, single-cell transcriptomics has been gradually applied in diverse aspects of animal research, offering an effective method to study the gene expression of high-throughput single cells of different tissues/organs in animals. In an unprecedented manner, researchers have identified cell types/subtypes and their marker genes, inferred cellular fate trajectories, and revealed cell‒cell interactions in animals using single-cell transcriptomics. In this paper, we introduce the development of single-cell technology and review the processes, advancements, and applications of single-cell transcriptomics in animal research. We summarize recent efforts using single-cell transcriptomics to obtain a more profound understanding of animal nutrition and health, reproductive performance, genetics, and disease models in different livestock species. Moreover, the practical experience accumulated based on a large number of cases is highlighted to provide a reference for determining key factors (e.g., sample size, cell clustering, and cell type annotation) in single-cell transcriptomics analysis. We also discuss the limitations and outlook of single-cell transcriptomics in the current stage. This paper describes the comprehensive progress of single-cell transcriptomics in animal research, offering novel insights and sustainable advancements in agricultural productivity and animal health. [ABSTRACT FROM AUTHOR]

Simple Summary: The use of black soldier fly (Hermetia illucens) and house fly (Musca domestica) as an alternative for farm animal feeding has very important implications for sustainability since it does not require the use of resources such as water and land for production, in addition to contributing to food security, especially in locations with a reduced supply of animal feed. Thus, the use of these insects as a source of nutrients can reduce the need for supplementation and the use of grains and forages. Dietary alternatives using insect-based products as an alternative for farm animal nutrition have been the object of study due to the high nutritional value of these feeds and the costs related to both their production and consequently their commercialization. Thus, the use of flies, especially larvae, has a high content of proteins and lipids (fat), as well as minerals and essential nutrients for development and growth, directly impacting the production of these animals, whether meat or milk. Therefore, the objective of this study was to compile data from the literature on the nutritional value of adults and larvae of Black soldier (Hermetia illucens) and housefly (Musca domestica) as a dietary alternative for animal feed. The Prisma checklist was used. After reviewing the data found in the literature, following the systematic review, it was noted that studies emerge that larvae of black soldier flies and domestic flies of the order Diptera obtain essential sources in the nutrition of ruminants, in addition to obtaining rapid digestibility, thus adhering to reproduction with high nutritional content, due to incident levels of protein, lipids, and minerals in M. domestica and Lucilia sericata, making it a target for inclusion in the diet of farm animals. In addition, it is concluded that both species are studied for their sustainable potential as well as for offering greater economic and nutritional viability when compared to ingredients present in production animal feed. [ABSTRACT FROM AUTHOR]

Simple Summary: A dynamic model was created to determine the nutritional requirements of lambs, aiming to simulate and describe their growth, nutrient requirements, and body composition. The model inputs include body mass, standard final mass, age, and dietary energy concentration. The results showed that the model accurately predicts the final body mass based on the inputs, with a coefficient of determination of 0.89 and a mean bias of 0.656 kg. The utility of this model extends to agricultural practitioners and livestock managers, providing valuable insights into forecasting final body mass and estimating the duration required to attain specific weights. Moreover, it serves as an educational tool elucidating the intricate interplay between nutrition, growth dynamics, and body composition in lambs. Thus, its implementation stands to enhance decision-making processes within feedlot lamb production, contributing to more informed and efficient management practices. A dynamic model has been developed to simulate aspects of feedlot lamb growth and body composition, including energy and protein requirements, growth rate, composition of gain, and body mass. Model inputs include initial body mass (kg), standard final mass (kg), age (days), and dietary energy concentration (Mcal·kg−1). The model was assessed as a decision support tool using a dataset of 564 individual measures of final body mass and diet energy. The simulations provide graphical and numerical descriptions of nutrient requirements, composition of gain, and estimates of animal performance over time. The model is accurate and precise, with a root mean squared error of 7.79% of the observed final body mass and a coefficient of determination of 0.89 when simulating the same variable. The model can be used as a reliable decision support tool to estimate final body mass and the days on feed required to reach a certain final mass with precision and accuracy. Moreover, the dynamic model can also serve as a learning tool to illustrate practical principles of animal nutrition, nutrient requirement relationships, and body composition changes. This model holds the potential to enhance livestock management practices and assist producers in making informed decisions about feedlot lamb production. [ABSTRACT FROM AUTHOR]

SUMMARY: Anthocyanins are natural pigments and dietary antioxidants that play multiple biological roles in plants and are important in animal and human nutrition. Low temperature (LT) promotes anthocyanin biosynthesis in many species including blood orange. A retrotransposon in the promoter of Ruby1, which encodes an R2R3 MYB transcription factor, controls cold‐induced anthocyanin accumulation in blood orange flesh. However, the specific mechanism remains unclear. In this study, we characterized two LT‐induced ETHYLENE RESPONSE FACTORS (CsERF054 and CsERF061). Both CsERF054 and CsERF061 can activate the expression of CsRuby1 by directly binding to a DRE/CRT cis‐element within the retrotransposon in the promoter of CsRuby1, thereby positively regulating anthocyanin biosynthesis. Further investigation indicated that CsERF061 also forms a protein complex with CsRuby1 to co‐activate the expression of anthocyanin biosynthetic genes, providing a dual mechanism for the upregulation of the anthocyanin pathway. These results provide insights into how LT mediates anthocyanin biosynthesis and increase the understanding of the regulatory network of anthocyanin biosynthesis in blood orange. Significance Statement: Anthocyanins are natural pigments and dietary antioxidants that play multiple biological roles in plants and are important in human nutrition. Low temperature is a necessary condition for anthocyanin accumulation in blood orange and the retrotransposon promoter of Ruby1 controls this process. Our results provide upstream insights into its cold response and enrich the understanding of the regulatory network of anthocyanin biosynthesis in blood orange. These results are of fundamental importance and great practical significance. [ABSTRACT FROM AUTHOR]

Microalgae are booming as a sustainable protein source for human nutrition and animal feed. Nevertheless, certain strains were reported to have robust cell walls limiting protein digestibility. There are several disruption approaches to break down the cell integrity and increase digestive enzyme accessibility. This review's intent is to discuss the digestibility of microalgae proteins in intact cells and after their disruption. In intact single cells, the extent of protein digestibility is chiefly related to cell wall structural properties (differing among strains) as well as digestion method and when added to food or feed protein digestibility changes depending on the matrix's composition. The degree of effectiveness of the disruption method varies among studies, and it is complicated to compare them due to variabilities in digestibility models, strains, disruption method/conditions and their consequent impact on the microalgae cell structure. More exhaustive studies are still required to fill knowledge gaps on the structure of microalgal cell walls and to find efficient and cost-effective disruption technologies to increase proteins availability without hindering their quality. [ABSTRACT FROM AUTHOR]