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Nowadays, the topic of sentiment analysis has attracted a wide range of artificial intelligence technologies suchas Natural Language Processing (NLP), neural network. Long Short-Term Memory (LSTM), a deep neural network, has proven the effiency in the task of sentiment classification. However, there is a shortage of semantic knowledge from such classification results. This paper introduces a sentiment analysis approach based on a knowledge model to discover hidden affected objects, which combines ONtology and SentiMent technology, named ONSEM. In order to confirm the efficiency of the ONSEM framework, an experiment on a sentiment analysis dataset was developed and then yielded promising experimental results.

The number of cells in tissues is controlled by cell division and cell death, and its misregulation could lead to pathological conditions such as cancer. To maintain the cell numbers, a cell-elimination process called apoptosis also stimulates the proliferation of neighboring cells. This mechanism, apoptosis-induced compensatory proliferation, was originally described more than 40 years ago. While only a limited number of the neighboring cells need to divide to compensate for the apoptotic cell loss, the mechanisms that select cells to divide have remained elusive. Here we found that spatial inhomogeneity in Yes-associated protein (YAP)-mediated mechanotransduction in neighboring tissues determines the inhomogeneity of compensatory proliferation in Madin-Darby Canine Kidney (MDCK) cells. Such inhomogeneity arises from the non-uniform distribution of nuclear size, and the non-uniform pattern of mechanical force applied to neighboring cells. Our findings from a mechanical perspective provide additional insight into how tissues precisely maintain homeostasis.

Developing organs are shaped, in part, by physical interaction with their environment in the embryo. In recent years, technical advances in live-cell imaging and material science have greatly expanded our understanding of the mechanical forces driving organ formation. Here, we provide a broad overview of the types of forces generated during embryonic development and then focus on a subset of organs underlying our senses: the eyes, inner ears, nose and skin. The epithelia in these organs emerge from a common origin: the ectoderm germ layer; yet, they arrive at unique and complex forms over developmental time. We discuss exciting recent animal studies that show a crucial role for mechanical forces in, for example, the thickening of sensory placodes, the coiling of the cochlea and the lengthening of hair. Finally, we discuss how microfabricated organoid systems can now provide unprecedented insights into the physical principles of human development.

Human skin uses millions of hairs and glands distributed across the body surface to function as an external barrier, thermoregulator and stimuli sensor. The large-scale generation of human skin with these appendages would be beneficial, but is challenging. Here, we describe a detailed protocol for generating hair-bearing skin tissue entirely from a homogeneous population of human pluripotent stem cells in a three-dimensional in vitro culture system. Defined culture conditions are used over a 2-week period to induce differentiation of pluripotent stem cells to surface ectoderm and cranial neural crest cells, which give rise to the epidermis and dermis, respectively, in each organoid unit. After 60 d of incubation, the skin organoids produce hair follicles. By day similar to 130, the skin organoids reach full complexity and contain stratified skin layers, pigmented hair follicles, sebaceous glands, Merkel cells and sensory neurons, recapitulating the cell composition and architecture of fetal skin tissue at week 18 of gestation. Skin organoids can be maintained in culture using this protocol for up to 150 d, enabling the organoids to be used to investigate basic skin biology, model disease and, further, reconstruct or regenerate skin tissue.

TÓM TẮT Đối với ung thư vú (UTV) xạ trị là điều trị bổ túc cần thiết giúp giảm tái phát tại chỗ tại vùng, gia tăng sống còn. Tuy nhiên, xạ trị có tác dụng phụ lên tim mạch nhất là với UTV trái. Nhiều nghiên cứu đã chứng minh biến chứng và tử vong do tim mạch tăng tỉ lệ thuận với liều trung bình lên tim. Các kỹ thuật xạ trị mới trong xạ trị ung thư vú trái giúp tối ưu hóa liều vào thể tích xạ và bảo vệ tốt hơn cơ quan lành, càng đòi hỏi độ chính xác cao khi đặt bệnh. Các sai số do thiết bị hoặc đặt bệnh sẽ dẫn đến nguy cơ quá liều dung nạp hoặc thiếu liều và có nguy cơ tái phát. Vì vậy, xạ trị đòi hỏi sự chính xác cao trong suốt quá trình từ mô phỏng đến lập kế hoạch và tiến hành xạ trị. Xạ trị ung thư vú trái phối hợp hít sâu nín thở (DIBH - Deep Inspiration Breath - hold) và hệ thống quản lý bề mặt quang học (OSMS - Optical Surface Management System) làm cho tim di chuyển ra xa hơn khỏi vú, thành ngực trong quá trình xạ trị, giúp giảm liều tim một cách rõ rệt, vẫn đảm bảo được liều xạ lên thể tích đích. Ưu điểm của OSMS ngoài áp dụng cho đặt bệnh nhanh không cần xăm dấu trên da còn giám sát thời gian thực trong toàn bộ quá trình điều trị. Khi nhịp thở của bệnh nhân vượt quá một ngưỡng nhất định (0,3cm) chùm tia bức xạ sẽ bị tắt để ngăn ngừa độc tính. Do đó, hiểu và nắm rõ lợi ích quy trình đặt bệnh giúp đạt mục tiêu điều trị đồng thời giảm thời gian đặt bệnh để người bệnh có tâm lý thoải mái hơn, giảm áp lực về số lượng bệnh, nhất là đối với các trung tâm xạ trị lớn. ABSTRACT BENEFITS OF LEFT BREAST CANCER RADIATION COMBINATION OF DEEP INSPIRATION BREATH - HOLD AND OPTICAL SURFACE MANAGEMENT SYSTEM OF TRUEBEAM MACHINE AT CANCER HOSPITAL HO CHI MINH CITY Background: For breast cancer, radiation therapy is an essential adjuvant treatment to help reduce local recurrence and increase survival. However, radiation therapy has adverse effects on the cardiovascular systemespecially for left breast cancer. Several studies have demonstrated that cardiovascular morbidity and mortality increase in proportion to the mean cardiac dose. New radiotherapy techniques in radiation therapy for left breast cancer help optimize dose to radiation volume and better protect healthy organs, which requires high accuracy when patient set - up. Errors due to equipment or patient set - up will lead to the risk of overdosage or underdosage and risk of relapse. Therefore, radiation therapy requires high precision throughout the process from simulation to planning and conducting radiation therapy. Radiation therapy for left breast cancer combined with Deep Inspiration Breath - hold (DIBH) and Optical Surface Management System (OSMS)causes the heart to move further away from the breast and chest wall during radiation therapy, helping to reduce the cardiac dose significantly, still ensuring the radiation dose to the target volume. The advantage of OSMS is that in addition to being applied for fastpatient set - up, without tattooing on the skin, it also provides real - time monitoring during the entire treatment process. When the patient’s breathing rate exceeds a certain threshold (0,3cm) the radiation beam is turned off to prevent toxicity. Therefore, understanding and understanding the benefits of patient set - up helps to achieve treatment goals, while reducing patient set - up time for more comfort, reducing pressure on the number of patients, especially for patients large radiotherapy center. Keyword: Radiation therapy for left breast cancer, Deep Inspiration Breath - hold, Optical Surface Management System, cardiovascular risk.

Studies of electric effects in biological systems, from the historical experiments of Galvani 1 and the ground-breaking work on action potential2 to studies on limb regeneration3 or wound healing4, share the common feature of being concerned with transitory behavior and not addressing the question of homeostasis. Here using a novel microfluidic device, we study how the homeostasis of confluent epithelial tissues is modified when a trans-epithelial electric potential (TEPD) different from the natural one is imposed on an epithelial layer. We show that epithelial fate is dependent on TEPD of few Volts/cm similar to the endogenous one. When the field direction matches the natural one, we can restore a perfect confluence in an epithelial layer turned defective either by E-cadherin knock-out or by weakening cell-substrate adhesion; additionally, the tissue pushes on the substrate with kilo-Pascals stress, inducing active cell response such as death and differentiation. When the field is opposite, homeostasis is destroyed by the perturbation of junctional actin and cell shapes, and the formation of dynamical mounds5, while the tissue pulls with similar strengths. Most of these observations can be quantitatively explained by an electro-hydrodynamic theory involving local electro-osmotic flows. We expect this work to motivate further studies on long time effects of electromechanical pathways with important tissue engineering applications.

The number of cells in tissues is tightly controlled by cell division and cell death, and misregulation of cell numbers could lead to pathological conditions such as cancer. To maintain cell numbers in a tissue, a cell elimination process named programmed cell death or apoptosis, stimulates the proliferation of neighboring cells. This mechanism is called apoptosis-induced compensatory proliferation, which was originally reported more than 40 years ago. While only a limited number of the neigboring cells need to divide to compensate for apoptotic cell loss, the mechanisms that select cells for undergoing division remain an open question. Here we found that the spatial inhomogeneity in mechanotransduction through a growth-promoting transcription co-activator Yes-associated protein (YAP) in the neighboring tissue, accounts for the inhomogeneity of compensatory proliferation. Such inhomogeneous mechanotransduction arises from the combination of the non-uniform distribution of nuclear size, which is inherent in tissues, and the non-uniform pattern of mechanical force applied to the neighboring cells upon apoptosis. Our findings from a mechanical perspective complement the current biochemical understanding of compensatory growth and provide additional insights into cellular functions of how tissue precisely maintains its homeostasis.

Human skin uses millions of hairs and glands distributed across the body surface to function as an external barrier, thermoregulator and stimuli sensor. The large-scale generation of human skin with these appendages would be beneficial, but is challenging. Here, we describe a detailed protocol for generating hair-bearing skin tissue entirely from a homogeneous population of human pluripotent stem cells in a three-dimensional in vitro culture system. Defined culture conditions are used over a 2-week period to induce differentiation of pluripotent stem cells to surface ectoderm and cranial neural crest cells, which give rise to the epidermis and dermis, respectively, in each organoid unit. After 60 d of incubation, the skin organoids produce hair follicles. By day ~130, the skin organoids reach full complexity and contain stratified skin layers, pigmented hair follicles, sebaceous glands, Merkel cells and sensory neurons, recapitulating the cell composition and architecture of fetal skin tissue at week 18 of gestation. Skin organoids can be maintained in culture using this protocol for up to 150 d, enabling the organoids to be used to investigate basic skin biology, model disease and, further, reconstruct or regenerate skin tissue.

International audience; The control of tissue growth, which is a key to maintain the protective barrier function of the epithelium, depends on the balance between cell division and cell extrusion rates [1, 2]. Cells within confluent epithelial layers undergo cell extrusion, which relies on cell-cell interactions [3] and actomyosin contractility [4, 5]. Although it has been reported that cell extrusion is also dependent on cell density [6, 7], the contribution of tissue mechanics, which is tightly regulated by cell density [8-12], to cell extrusion is still poorly understood. By measuring the multicellular dynamics and traction forces, we show that changes in epithelial packing density lead to the emergence of distinct modes of cell extrusion. In confluent epithelia with low cell density, cell extrusion is mainly driven by the lamellipodia-based crawling mechanism in the neighbor non-dying cells in connection with large-scale collective movements. As cell density increases, cell motion is shown to slow down, and the role of a supracellular actomyosin cable formation and its contraction in the neighboring cells becomes the preponderant mechanism to locally promote cell extrusion. We propose that these two distinct mechanisms complement each other to ensure proper cell extrusion depending on the cellular environment. Our study provides a quantitative and robust framework to explain how cell density can influence tissue mechanics and in turn regulate cell extrusion mechanisms.

This paper will aim at discovering how the higher education institutions use the social media and what are some of the factors affect the adoption or use of social media like Facebook by higher education institutions in Vietnam. To evaluate the efficiency, the research used the model Data Envelopment Analysis (DEA). The purpose of using DEA is because the method is able to apply more parameters of input and output to analysis of evaluate which university (s) (units) under investigation is the most effective in using Facebook to promote their brand, and to compare other universities for it. The proposed model by the researcher is applied to assess the Facebook pages of top 10 universities in Vietnam. The result of the evaluation shows that all the investigated universities focused on improving their brand by interacting with as many people as possible. The number of Likes and Comments in the university's Facebook pages indicated that students and parents use Facebook to learn more about the courses and facilities offered by the learning institutions. Therefore, the DEA model is an effective tool that helps to evaluate the efficiency of the business in the social network like Facebook in promoting their brands because of its uses multiple variables, and also it does not necessarily require the relationship between the inputs and outputs.

The shaping of a multicellular body and repair of adult tissues require fine-tuning of cell adhesion, cell mechanics and intercellular transmission of mechanical load. Adherens junctions (AJs) are the major intercellular junctions by which cells sense and exert mechanical force on each other. However, how AJs adapt to mechanical stress and how this adaptation contributes to cell-cell cohesion and eventually to tissue-scale dynamics and mechanics remains largely unknown. Here, by analyzing the tension-dependent recruitment of vinculin, α-catenin and F-actin as a function of stiffness, as well as the dynamics of GFP-tagged wild-type and mutated α-catenins, altered for their binding capability to vinculin, we demonstrate that the force-dependent binding of vinculin stabilizes α-catenin and is responsible for AJ adaptation to force. Challenging cadherin complexes mechanical coupling with magnetic tweezers, and cell-cell cohesion during collective cell movements, further highlight that tension-dependent adaptation of AJs regulates cell-cell contact dynamics and coordinated collective cell migration. Altogether, these data demonstrate that the force-dependent α-catenin/vinculin interaction, manipulated here by mutagenesis and mechanical control, is a core regulator of AJ mechanics and long-range cell-cell interactions.Summary statementCombining cell biology and biomechanical analysis, we show here that the coupling between cadherin complexes and actin trough tension-dependent α-catenin/vinculin association is regulating AJ stability and dynamics as well as tissue-scale mechanics.

Foreign direct investment (FDI) and corporate social responsibility (CSR) spending are one of the major factors in improving sustainable economic development of a country. Therefore, this study focuses on the multi criteria application of FDI and sustainability factors (CSR spending) in various developing countries to explore its impact and decision making for sustainable economic growth. The study uses a case study methodology whereby FDI, exchange rate, and CSR expenditure data from 20 countries were used to assess the efficiency in sustainable economic growth. Data were collected from the World Bank for 20 Asian and African developing countries during 2012&ndash, 2017 and analyzed using GM (1,1), mean absolute percentage error (MAPE), Malmquist productivity index (MPI)-data envelopment analysis (DEA), and the slacks-based measure of efficiency (SBM) model. Correlation analysis is used to find the relationship for FDI, CSR, exchange rate, gross domestic product (GDP), and GDP per capita (GDPPC). The results of the Malmquist productivity index and the frontier effect clearly highlight that a few countries have witnessed a great improvement in terms of productivity and technological progression. Therefore, the decision makers must adopt the model of those countries with respect to sustainable development of the nation. This study helps developing nations as well as researchers to benchmark efficient countries and follow their strategies to develop a new one for utilizing FDI and CSR spending in sustainable economic development. The study also helps policy makers in multi criterion application of FDI and CSR for decision making in economic development.

International market selection for conducting exports is based on various trade and economic factors. It is very important to understand how to efficiently trade with another country and to maintain consistency. However, such a consistency is not possible in the case of economics. Therefore, using an efficiency measure helps us to explore and benchmark a country’s exportation businesses. The main goal of this study is to develop an integrated data envelopment analysis (DEA) model to explore the most productive manner through which Vietnam exports goods to other countries. Exploring the most productive export business will help us achieve another goal of this study, which is the selection of international market efficiencies. The variables used for this analysis include the exports, total exports, import tariff, dollar exchange, and the ease of doing business. Based on the data collected from an international organization, on the 15 leading export markets in Vietnam, this study applied integrated DEA, which combines the super slack-based measure (Super SBM) and Malmquist Productivity Index (MPI) analysis to evaluate the export market efficiency. The findings show that for the selection of the export market, the three countries that were the most consistently efficient during 2014–2017 were Malaysia, Singapore, and the United States. This study indicates that the selection of international markets for developing countries should comply with low tariff rates, low exchange rates, and a higher ease of doing business in order to improve the gross domestic product (GDP) and economy of the country.

A nanocomposite of multiwalled carbon nanotubes (CNTs) and poly(3-hexylthiophene) (P3HT) was prepared by grafting P3HT uniformly on the surface of CNTs (P3HT/P3HT-g-CNT) via a "grafting from" method with the coating thicknesses controlled. It was found that as the coating thickness decreased, the crystallinity of the P3HT decreased, along with significant red-shifting of Raman that signified alterations of chain conformation. Furthermore, although the photoluminescence (PL) peak remained unchanged when grafted on CNTs, modifications of P3HT energy gap was observed, indicating variations of vibronic levels arising from the grafting. Moreover, broadening of the PL emission took place that suggested decreasing of lifetimes of the photo-generated species when grafted on CNTs. Bilayer photovoltaic devices with the (6,6)-phenyl C61-butyric acid methyl ester as the electron acceptor have shown that the nanocomposite P3HT/P3HT-g-CNT performed much more efficiently as the electron donor, in both photocurrent density and power conversion efficiency, compared with the neat P3HT.

Firms gain competitive advantage by engaging customers and value network partners in co-creation activities. Business models that integrate value co-creation in a network are recognized as an essen...

Collective migration of cells is of fundamental importance for a number of biological functions such as tissue development and regeneration, wound healing and cancer metastasis. The movement of cell groups consisting of multiple cells connected by cell-cell junctions depends on both extracellular and intercellular contacts. Epithelial cell assemblies are thus regulated by a cross-talk between cell-substrate and cell-cell interactions. Here, we investigated the onset of collective migration in groups of cells as they expand from a few cells into large colonies as a function of extracellular matrix (ECM) protein coating. By varying the amount of ECM presented to the cells, we observe that the mode of colony expansion, as well as their overall geometry, is strongly dependent on substrate adhesiveness. On high ECM protein coated surfaces, cells at the edges of the colonies are well spread exhibiting large outward-pointing protrusive activity, whereas cellular colonies display more circular and convex shapes on less adhesive surfaces. Actin structures at the edge of the colonies also show different organizations with the formation of lamellipodial structures on highly adhesive surfaces and a pluricellular actin cable on less adhesive ones. The analysis of traction forces and cell velocities within the cellular assemblies confirm these results. By increasing ECM protein density, cells exert higher traction forces together with a higher outward motility at the edges. Furthermore, tuning cell-cell adhesion of epithelial cells modified the mode of expansion of the colonies. Finally, we used a recently developed computational model to recapitulate the emergent experimental behaviors of expanding cell colonies and extract that the main effect of the different cell-substrate interactions is on the ability of edge cells to form outward lamellipodia-driven motility. Overall, our data suggest that switching behaviors of epithelial cell assemblies result in a tug-of-war between friction forces at the cell-substrate interface and cell-cell interactions.